56 results about "Dextranase activity" patented technology

The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal β-1,4-xylosidic linkages or endo-β-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

This invention relates to detergent compositions comprising bacterial alkaline enzymes exhibiting endo-beta-1,4-glucanase activity (E.C. 3.2.1.4) and an ethoxylated polymer selected from the group consisting of polyethylene glycol/vinyl acetate graft copolymer; a ethoxylated (and optionally propoxylated) polyethyleneimine; a zwitterionic and ethoxylated polyamidoamine; ethoxylated (and optionally propoxylated) comb polycarboxylate; and mixtures thereof.

The invention discloses an acid-resistant pseudomonas koreensis strain CLP-7 which is capable of preventing diseases and promoting growth, and can be used for biocontrol. The acid-resistant pseudomonas koreensis strain CLP-7 is preserved at China General Microbiological Culture Collection Center, on 27th, October, 2016, and the preservation number is CGMCC No.13204. The antibacterial spectrum of the acid-resistant pseudomonas koreensis strain CLP-7 is relatively large; the acid-resistant pseudomonas koreensis strain CLP-7 is capable of inhibiting growth of Phytophthora parasitica var. nicotianae, Ralstonia solanacearum, and Alternaria alternata Keissler; the antagonistic activity under acidic conditions is high, the acid-resistant pseudomonas koreensis strain CLP-7 is capable of producing siderophore, possesses protease and glucanase activity, and potassium releasing capacity. The results of biocontrol pot experiment show that the acid-resistant pseudomonas koreensis strain CLP-7 is capable of preventing under acidic soil conditions, and promoting growth and chlorophyll synthesis of tobacco seedlings in acidic soil, so that the acid-resistant pseudomonas koreensis strain CLP-7 and microorganism bacterium agents of the acid-resistant pseudomonas koreensis strain CLP-7 can be used for preventing tobacco fungi and bacterial root and stem diseases under continuous cropping or acidic soil conditions effectively, and is high in application value.

The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.

A process using a multicomponent enzyme preparation to treat screened once refined pulps and reduces the specific energy consumption and/or increasing production while maintaining or increasing handsheet physical properties. The enzyme preparation has a major endoglucanase activity, a significant mannanase activity and a relatively small cellobiohydrolase activity. This enzyme mixture is prepared from a genetically modified strain of Trichoderma reseii.

This invention relates to laundry detergent compositions comprising bacterial alkaline enzymes exhibiting endo-beta-1,4-glucanase activity (E.C. 3.2.1.4) and modified cellulose derivatives.

The invention discloses a detection method for disease resistance of cotton verticillium wilt. The detection method comprises the following steps: inoculating a verticillium wilt bacterium into a cotton seedling, detecting the activity of chitinase and/or activity of dextranase in the inoculated cotton seedling; and judging the disease resistance of cotton verticillium wilt according to the activity of chitinase and/or activity of dextranase in the inoculated cotton seedling. According to the detection method, the disease resistance of cotton verticillium wilt is judged by inoculating the verticillium wilt bacterium into the cotton seedling and detecting the activity of chitinase and/or the activity of dextranase in leaves of the inoculated cotton seedling. The method can be used for quickly and accurately acquiring the disease resistance of cotton verticillium wilt in the seedling growing stage without waiting for the cotton seedling to disease. A plant with good disease resistance or tolerance to diseases can be transplanted according to the detection result of the method. Through verification and screening in field morbidity, the anti-disease cotton plants and lines are obtained. The detection method is capable of conveniently obtaining single strains and materials with good disease resistance or tolerance to diseases from the seedling growing stage for direct high yield and quality breeding so as to accelerate the variety breeding process, so that the detection method is suitable for being popularized and applied.

The invention discloses a beta-1, 3-1, 4-dextranase and an encoding gene thereof. The beta-1, 3-1, 4-dextranase provided by the invention is protein in 1 or 2 or 3 as follows: 1. the protein which is formed from an amino acid sequence shown in 19th-314th bit of a sequence 2 in a sequence table; 2. the protein which is formed from the amino acid sequence shown in the sequence 2 in the sequence table; and 3. the protein which is formed from an amino acid residue sequence of the sequence 2 in the sequence table through the substitution and/or deletion and/or addition of one or more amino acid residues, has the activity of the beta-1, 3-1, 4-dextranase and is derived from 1. A recombined pichia formed by leading the encoding gene of the protein into pichia also belongs to the protecting range of the invention. Experiments prove that the highest enzyme activity of a recombined pichia fermented liquid supernatant can be 55,300U/mL, and crude protein is 9.1g/L. The optimum reaction temperature of the beta-1, 3-1, 4-dextranase of the invention is 70 DEG C, and the optimum pH value is 7.0. The beta-1, 3-1, 4-dextranase has great generalization and application potential in production.

The present invention discloses an efficient, selective and economical method for producing a cereal dispersion of a non-dairy ready-to-use milk substitute with intact beta-glucans, proteins and natural sugars while maintaining natural Aroma and flavor of the grain. The method comprises treating a cereal substrate suspension with an enzyme preparation comprising at least one hydrolase capable of hydrolyzing α-glucosidic bonds and being free of glucanase activity and protease activity. The hydrolytic enzyme may be selected from alpha-amylase, beta-amylase, amyloglucosidase and pullulanase, provided that when the enzyme preparation contains alpha-amylase or beta-amylase, it is always combined with at least one A mixture of other α-glycoside hydrolases. When beta-amylases and alpha-amylases are selected, they are used as a mixture, ie introduced at the same time, to provide accelerated enzymatic hydrolysis, thereby reducing the amount of enzyme required compared to the use of these two enzymes alone. In addition to the above-mentioned hydrolase, the enzyme preparation of the present invention further includes isomerase, such as glucose isomerase.

The present invention relates to compositions that can be used in hydrolyzing biomass such as compositions comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity, methods for hydrolyzing biomass material, and methods for reducing viscosity of biomass mixture using a composition comprising a polypeptide having glycosyl hydrolase family 61/endoglucanase activity.

A process prepares a beverage or beverage component. The process includes an enzymatic treatment of brewer's spent grain including addition of one or a combination of enzymes with alpha-amylase, gluco-amylase, cellulase, xylanase, protease and/or beta-glucanase activity and fermentation by a strain of lactic acid bacteria. The combination of enzymes and enzymatic treatment conditions is such that the lactic acid bacteria produce 4.5 g/L lactic acid and metabolise sugar such that the resulting fermented broth contains less than 2.5% w/w and preferably less than 0.5% w/w residual sugar or the lactic acid bacteria produce 4.5 g/L lactic acid and metabolise sugar such that the resulting fermented broth contains at least 2.5% w/w residual sugar.

A method, composition and system for controlling termites wherein single carbohydrate-based compounds are used as both cellulase inhibitors and feeding stimulants. Di-saccharides, cellobioimidazole (CBI), fluoro-methyl cellobiose (FMCB), and mono-saccharides, fluoro-methyl glucose (FMG) and analogs thereof inhibit termite cellulose digestion, which leads to starvation or stimulates termite feeding to cause mortality. CBI, FMCB and FMG were tested against enzyme fractions that represented endogenous (foregut/salivary gland/midgut) and symbiotic (hindgut) termite cellulases in vitro and in vivo. Feeding stimulation by di-saccharides results in greater cellulase inhibitor intake throughout midrange concentrations (1 mM-10 mM), which is associated with significant termite mortality. In contrast, the monosaccharide inhibitor, FMG did not stimulate feeding, but did inhibit feeding at concentrations above 1 mM, causing mortality. With modification to create longer β-glycosidic chain lengths, the cellulase inhibitors identified herein can also be targeted to endoglucanase activity for increased efficacy and use as novel termite control compositions.

The invention relates to a process for the production of wort, comprising the enzymatic treatment of gist in up to 100% unmalted (grain) form, for further processing into high quality beverage products. By the addition of a combination of exogenous enzymes (α-amylase, isoamylase/pullulanase, FAN generating activity (proteases) and beta-glucanase activity) to the mash and by the simultaneously thermal activation of the maltose-generating endogenous β-amylase, it is possible to obtain a wort based on up to even 100% barley. The invention further relates to a process for the production of a high quality beer or beer product and to the high quality beer produced according to the process.

The invention relates to a method for prevention and treatment of diseases of longan fruits, wherein the method comprises the steps: (1) before picking, from the beginning of a longan young fruit stage, spraying a disease prevention and treatment liquid on longan; (2) when the maturity of the longan fruits is 80-90%, picking the longan fruits; and (3) soaking the longan fruits after picking in slightly acidic electrolyzed water, soaking for 10-15 min, then taking out, draining water, then carrying out fresh-keeping packaging on the longan fruits after draining, placing the packaged longan fruits under conditions of the temperature of 25-30 DEG C and the relative humidity of 85%-90%, and storing. The method for prevention and treatment of diseases of the longan fruits can improve the longan peel chitinase activity, beta-1, 3-glucanase activity, and the activity of phenylalanine ammonia lyase and other decrease-resistant related enzymes, and delays the decrease of the total phenolic content, so as to effectively control the occurrence of the diseases of postharvest longan fruits.

A method for suppressing a reduction in an endoglucanase activity in the presence of a surfactant, characterized by modifying a protein having the endoglucanase activity in which the N-terminus is an amino acid other than pyroglutamic acid, to a protein having the N-terminus of pyroglutamic acid, is disclosed. Further, a modified protein having an endoglucanase activity wherein the N-terminal amino acid is converted into pyroglutamic acid by an amino acid modification, a polynucleotide encoding the protein, an expression vector comprising the polynucleotide, a host cell transformed with the expression vector, and a process for producing the protein by cultivating the host cell, are disclosed.