The invention belongs to the field of feed, and particularly relates to chicken feed and a preparation method and application thereof. The chicken feed comprises the following materials and additives of rice bran, corn flour, flower meal, soybean meal, calcium hydrophosphate, mountain flour, edible salt, premix, fish bone dust, plant extracts, cottonseed meal, sweet potato flour, wheat middling, microelements, vegetable oil, complex enzyme preparations, ferrous sulfate, zinc sulfate, pine needle meal, silkworm chrysalis meal, papaya powder and groundnut meal. The invention further provides the preparation method for the chicken feed and the fertilization application of the chicken feed in the growing period of dendrobium officinale. According to the chicken feed and the preparation method and application thereof, natural elements are sufficiently used, beneficial components are extracted and allocated evenly, raw materials in the used formula are wide in sources and high in nutritive value, the disease resistant ability of chickens is improved, the egg laying rate of the chickens is increased, the death rate of the chickens is reduced, and feeding cost is reduced; and after the chickens are eaten by people, the health care effects of disease preventing, disease resistance and human body immunity improving are achieved.