197 results about "Carbofuran" patented technology

The present invention discloses an insecticide-fertilizer particulate agent and a preparation method thereof. The insecticide-fertilizer particulate agent comprises an insecticide, a bactericide, a fertilizer and an auxiliary agent. The insecticide is composed of one or more components selected from diazinon, thiamethoxam, imidacloprid, clothianidin, chlopyrifos, phoxim, carbofuran and carbosulfan. The bactericide is composed of one or more components selected from azoxystrobin, pyraclostrobin, fludioxonil, metalaxyl, methylimidazole bacteria aniline, thifluzamide and mancozeb. The fertilizer is composed of one or more components selected from urea, ammonium sulfate, potassium sulfate, potassium chloride, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium polyphosphate, potassium nitrate, zinc sulfate, manganese sulfate, magnesium sulfate, borax, boric acid, ferrous sulfate, copper sulfate, ammonium molybdate, ethylene diamine tetraacetic acid, calcium nitrate, potassium humate, organic matter, amino acid and soybean meal. The insecticide-fertilizer particulate agent is applied during the crop sowing period and can be used for preventing and controlling crop underground pests and diseases at the rootstocks of crops.

The present invention relates to a method for analyzing residual agricultural chemicals which comprises the steps of acting a reduced glutathione as a reactive substrate and a glutathione transferase serving as a catalyst for the reaction on a carbofuran derivative or a methomyl derivative as a carbamate type agricultural chemical of a new series to thus derivatize the agricultural chemical into a substance having a high choline esterase-inhibitory activity; reacting the substances formed through the derivatization reaction with a choline esterase; and then detecting the presence of the agricultural chemical as the new series of carbamate type one included in a sample to be examined on the basis of the changes in the choline esterase activity thus detected. The method of the present invention may serve as a powerful tool for the detection of the residual agricultural chemicals in grains such as rice and the detection of the content of agricultural chemicals remaining in agricultural products such as vegetables and fruits.

A nano colloidal golf linked immunoassary for detecting carbofuran includes such steps as coupling the immunized semi-antigen of carbofuran with BSA, synthesizing artificial immunoantigen compound, immunizing animal to prepare specific high-valence BFNH antibody, separating and purifying, linking the nano-class colloidal golf to said specific antibody, solidifying said linked compound, carbofurancoupled by egg albumin and sheep's mouse IgG antibody on a carrier, and for semi-quantitative analysis. Its advantage is high sensitivity and speed.

The invention relates to an acetylcholinesterase biosensor and an application thereof, and belongs to the technical field of electrochemical detection. The biosensor is produced through the following steps: 1, preparing a MnO2-nanoflakes composite nano-material and a Pd-Pt@MnO2-nanoflakes composite nano-material from KMnO4, CTAB, PdCl2, PEG400, H2PtCl6, sodium citrate and sodium borohydride used as initial raw materials through a water bath technology; and 2, immobilizing acetylcholinesterase by using chitosan (CS), and immobilizing acetylcholinesterase (AChE) on the surface of a Pd-Pt@MnO2 modified glassy carbon electrode through adopting physical adsorption to construct the electrochemical biosensor. The biosensor adopting an electrochemistry and enzyme sensing combination technology realizes high-sensitivity detection of methyl parathion and carbofuran pesticides, has the advantages of small amount of a required sample, short detection time, high sensitivity and low cost, and is suitable for analyzing and detecting pesticide residual.

The invention provides an ELISA (enzyme linked immunosorbent assay) kit for detecting carbofuran. The ELISA kit comprises an ELISA plate coated with a carbofuran conjugated antigen, a carbofuran monoclonal antibody, an enzyme labeled antibody, a carbofuran standard solution, a substrate color development solution, a stop buffer, a cleaning solution and a re-dissolution solution. The invention further discloses a method for applying the ELISA kit to detection of the carbofuran. The method comprises the following steps: a sample is pretreated firstly, then the kit is used for detection, and a detection result is analyzed finally. The ELISA kit can be used for detecting the content of the carbofuran in vegetables and tea leaves, the operation is simple and convenient, the cost is low, the sensitivity is high, on-site supervision can be realized, and the kit is suitable for screening of a large quantity of samples.

The invention relates to a seed coating agent which comprises benfuracarb of insecticide as an active componet and high polymers like polythene alcohol or polypropylene acidamide as film-builders. With additives said seed coating agent can be processed into water-emulsion, hung-emulsion and dry powered type; the seed coating agent can be fixed to be insecticide and bactericide combined seed coating agent with additional bactericides as thiram, tebuconazole, N-butyl pertluorooctane sulfonamide, metalaxyl, carbendazol, prochloraze or seedvax as second active component. Said seed coating agent is mainly used to prevent aphid of corn, soja, paddy or cotton and a plurality of underground pest; and the combined type can treat black silk disease, kernel smut, stem rot in seedling stage and anthrax.

The invention discloses a closed prevention and control method for longhorn beetles. The method comprises the following steps of identifying a trunk damaged by longhorn beetles, pushing aside the soil 5 to 10cm below the root neck to find a channel of the longhorn beetle, penetrating the channel by using an iron wire, injecting organic phosphorous insecticides into the channel, coating carbofuran on and around the root neck of a seedling or a tree, and binding up the root neck by using a plastic film. By adopting the method, the effect of killing larvae of the longhorn beetle reaches 100 percent, the attack of longhorn beetles can be continuously controlled for three years, and the huge economic loss, caused by the attack of longhorn beetles, of seedlings and trees can be avoided. The method is easy to implement, and the medicines are readily available and easy to prepare and have obvious effect.

The invention provides a high-yield cassava cultivation technology. The high-yield cassava cultivation technology is characterized in that firstly, the planting land is a dry land; secondly, deep ploughing and soil preparation are conducted, and base fertilizer is applied sufficiently; thirdly, good varieties are selected and used; fourthly, thick seed stems are selected and used as cuttage seeds, wherein the seed stems should have vitality and are cut off to be 10-15 centimeters long, and the cut seed stems are soaked in ambam or lime water for disinfection; fifthly, planting is conducted at proper time, wherein when the daily average temperature is above 15 DEG C, planting can be conducted, and manure is applied and buried between every two seed stems; sixthly, growing period management includes filling of the gaps with seedlings, thinning, weeding and after-manuring; seventhly, pest control is conducted, wherein bisultap or carbofuran and fine soil are mixed and applied, or methamidophos is used for trapping and killing pests, and application of the fertilizer is stopped two months before harvesting; eighthly, harvesting is carried out at proper time in autumn, and the root yield and starch content will be lowered if harvesting is conducted too early or too late.

The invention provides sphingosine monoucleosis WHSC-8T having the efficient degrading effect on cypermethrin pesticide residues and an application of the sphingosine monoucleosis. A bacterial strain is preserved in the typical culture collection center of China on November 18, 2014, and the collection serial number is CCTCC NO: M2014575. The sphingosine monoucleosis has the remarkable degrading effect on the pesticide residues of cypermethrin, phoxim, chlorpyrifos, isoprocarb, carbofuran and the like, and the rate of degrading the cypermethrin is more than 91.3%. The pesticide residue degrading bactericide has the advantages of being low in production and use cost, convenient to use, good in removing effect and suitable for being used and popularized in grain, oil and vegetable production export bases across the country or areas with pollution-free food brand marks in a large-area mode.

This invention discloses one enzyme immune agent base to test carbofuran, which comprises base body and enzyme label board, which is characterized by the following: anti-carbofuran antigen, horse radish hyperoxide enzyme label antigen, carbofuran standard liquid, bottom liquid, bottom buffer liquid and terminal liquid, wherein, the said enzyme standard board covers anti-carbofuran antigen abnormal second antigen. This invention enzyme immune agent case uses second antigen covering enzyme standard board.

The invention discloses yield-increasing fertilizer used for wheat planting. The yield-increasing fertilizer used for the wheat plant is prepared from the following raw materials in parts by weight: 60-120 parts of livestock manure, 30-50 parts of plant ash, 5-15 parts of cassava residue, 2-8 parts of soybean meal, 1-5 parts of potassium mineral, 2-4 parts of wheat straw, 3-6 parts of castor cake, 1-5 parts of furfural residue, 1-3 parts of nitrogenous fertilizer, 2-4 parts of boric acid, 4-8 parts of ammonium nitrate, 2-9 parts of urea, 4-6 parts of zinc sulfate, 2-6 parts of magnesium-potassium sulfate, 3-4 parts of calcium superphosphate, 1-5 parts of paclobutrazol, 2-5 parts of carbofuran, 4-5 parts of metalaxyl and 2-6 parts of a soil conditioner. The yield-increasing fertilizer used for wheat planting can be used for effectively increasing the yield and quality of wheat.

Enzyme immunoassay method for detecting Kebaiwei includes (1) synthesizing half antigen and artificial antigen; (2) preparing polyclonal antibody, monoclonal antibody, or gene engineering antibody of possessing specificity to Kebaiwei; (3) preparing enzyme labeled antigen; (4) using second antibody to coat enzyme labeled plate and close the plate to save Kebaiwei; reaction is carried out between anti Kebaiwei antibody and second antibody and fixed on the enzyme labeled plate; (5) washing out free object, adding sample to be tested, and enzyme labeled antigen or Kebaiwei labeled sample, and enzyme labeled antigen; (6) washing out free object, adding substrate of enzyme and developer. Intensity of enzyme promoting color reaction is proportional to quantity of enzyme labeled antigen bound on antibody, and is inversely proportional to content of Kebaiwei in sample. Features are: long term storage, increasing sensitivity, accuracy, simple and quick test operation.

The invention discloses a liquid chromatography-tandem mass spectrometry method for measuring 18 semi-volatile organic pollutants in water. According to the method, a clean water sample such as surface water, drinking water, and the like is simply filtered by a 0.22 [mu]m micro-porous filter membrane, and then a liquid chromatography-mass spectrometry instrument is used to separate and detect the following 18 compounds in water: aniline, benzidine, acrylamide, picric acid, carbaryl, deltamethrin, microcystic toxin-LR, dibutyl phthalate, bis(2-ethylhexyl) phthalate, dichlorvos, trichlorphon, parathion, methyl parathion, malathion, dimethoate, demeton, atrazine, and carbofuran. The provided method can detect many target substances. The detection range of the method is 0.001 to 0.49 [mu]g / L, the relative standard deviation is 0.7 to 15.4%; and the analysis method has the advantages of high sensitivity, quick analysis speed, little pollution, and simple and efficient pretreatment, is especially suitable for standard analysis of drinking water source monitoring, and solves the problems of bad conformability, large labor strength, and low analysis efficiency of the conventional standard method.

The invention provides a carbofuran and isoprocarb double joint inspection immune fluorescent test paper strip, a kit and application thereof. The test paper strip comprises a bottom plate, a sample pad, a nitrocellulose membrane and a water-absorbing pad; the sample pad, the nitrocellulose membrane and the water-absorbing pad are adhered to the bottom plate sequentially; a first detection line bound with a carbofuran hapten-vector protein conjugate, a second detection line bound with an isoprocarb hapten-vector protein conjugate and a quality control line bound with a goat anti-mouse polyclonal antibody are arranged on the nitrocellulose membrane sequentially from front to back according to the sample flowing direction; and the kit comprises the test paper strip and a reaction cup filled with a carbofuran fluorescent microsphere marked antibody and an isoprocarb fluorescent microsphere marked antibody. The carbofuran and isoprocarb double joint inspection immune fluorescent kit can realize detection of carbofuran and isoprocarb residues simultaneously, has high detection accuracy, has the characteristics of high sensitivity and high specificity, can realize simple and rapid detection, and is simple and convenient to operate and suitable for large-scale screening.

The invention relates to a composite drug, which comprises carbofuran and high-effect cypermethrin at 1:2-20:1. The invention can effectively kill pests.

The invention discloses an organic capsule fertilizer dedicated for basil and a preparation method thereof. The organic capsule fertilizer dedicated for basil comprises the following ingredients in parts by weight: 15-20 parts of coal ash, 5-7 parts of borax, 4-7 parts of rare earth element, 30-35 parts of rape seed cake, 15-20 parts of decoction dregs, 23-26 parts of plant straw, 5-7 parts of corn powder, 5-8 parts of cotton seed hull, 2-4 parts of compound fungicide, 0.04-0.06 part of diethyl aminoethyl hexanoate, 6-9 parts of common superphosphate, 6-10 parts of potassium sulfate, 8.6-10.5 parts of urine, 0.6-1.0 part of carbofuran and 0.5-0.7 part of Taghigaren. According to the organic capsule fertilizer dedicated for basil, the use ratio of the fertilizer is improved, the basil is obviously accelerated to grow, the basil potassium is improved, the flowering phase is prolonged, and the organic capsule fertilizer has better economic and social benefit.

The invention discloses a preparation method of carbofuran molecularly imprinted microspheres, comprising the following steps of: dissolving template molecules and a functional monomer into a pore-forming agent solution containing a cross-linking agent and an initiator so as to obtain a reaction mixture, carrying out ultrasonic degassing, sparging nitrogen for deoxygenation, carrying out polymerization in a sealed state, carrying out centrifugation after complete reaction so as to obtain a white powdery polymer, carrying out Soxhlet extraction respectively by the adoption of a methanol acetate mixed liquor and methanol as extraction solvents, drying under vacuum, and removing the solvents so as to obtain the carbofuran molecularly imprinted microspheres. The invention also discloses the carbofuran molecularly imprinted microspheres prepared by the preparation method and an application thereof. The carbofuran molecularly imprinted microspheres obtained in the invention has specific selectivity for carbofuran in agricultural products, can be used to detect residual pesticides on agricultural products rapidly, sensitively, accurately and efficiently, and also can be used as a solid-phase extraction filler.

The invention aims at providing a corn high-yield planting method. The corn high-yield planting method includes the following steps: (1) selecting loose and good-transmitting soil, and adding organic fertilizer into the soil to enable the soil to serve as nutrient soil; (2) selecting organic corn seeds, airing the organic corn seeds for 3-5 hours, then soaking the organic corn seeds with a nutrient solution for 12-15 hours, and conducting accelerating germination for 1-2 days; (3) sowing the organic corn seeds obtained after accelerating germination, wherein in each sowing line, three holes serve as one planting set; in each planting set, one plant is planted into one hole; 3-5 sowing lines serve as one planting unit; the sowing lines in each planting unit are arranged in a disordered mode; in each planting unit, the first planting sets in all lines are arranged in a disordered mode in the manner that the distances between the first planting sets and a planting line are greatly increased by 3 cm-5 cm, and 5 g-10 g of carbofuran is applied each square meter after sowing is carried out; (4) field management is carried out on the sowed organic corn; (5) the organic corn is harvested and threshed. By means of the planting method, defects in the prior art are overcome, and the corn high-yield planting method has the advantages of preventing lodging, promoting nutrient absorption and increasing the corn yield.

Disclosed is a biochemical long-acting soybean seed-coating agent which comprises the following constituents (by weight portions): auxiliary agent 10%, carbofuran 8%, carbendazol 12%, thiram 15%, water 54%, pix 0.115%, Paclobutrazol 0.015%, op emulsifying agent 0.5%, phosphoric acid 0.5%, phosphor and potassium solubilization bacteria 108 units / ml.

The invention relates to the technical field of chemical pesticide test, and particularly relates to a method for testing carbofuran in carbamic acid ester pesticides, in particular to a method for testing trace carbofuran. With flour, zinc meso-tetraphenylporphine solution and phosphate buffer with the pH value of 6.5 to 7.25 as materials, the method respectively prepares zinc meso-tetraphenylporphine-plant esterase compound solution and sample mixed solution, then respectively tests the absorption spectrum curves of the zinc meso-tetraphenylporphine-plant esterase compound solution and the sample mixed solution on light waves with the wavelength of 300nm to 750nm, and compares the two tested spectrum curves; if the two spectrum curves are identical, then N, N-dimethylformamide cleaner sample to be tested does not contain carbofuran, or the content of carbofuran exceeds the test limit of the method; and if the two spectrum curves are not identical, then the sample to be tested contains carbofuran. The test cost of the method is low, the method is applicable to a wide range of regions, the precision, repeatability and stability of the test result are high, field tests can be carried out as well, the method is easy to operate, and moreover, the affection of the environment on the test result is little.

The invention discloses a planting method capable of improving sugariness of Kupla sweet corn. The planting method comprises the following steps of selecting land which is flat in terrain, fertile in earthiness, good in permeability and good in water retention, and has irrigation and drainage conditions; selecting planting geographic climatic conditions, wherein the mean air temperature throughout the year is 17-22 DEG C, the average temperature from June to September is between 19-23 DEG C, the mean air temperature of January is 8 DEG C or above, the frost-free period lasts for 250 days or above, the annual sunshine duration is 1800-2100 hours, the annual rainfall is 1000-1800mm, and the relative humidity of air is 75-90%; and performing soil preparation, classifying seeds, performing sowing, performing field management, and performing harvesting so as to obtain the Kupla sweet corn. According to the planting method disclosed by the invention, an appropriate compounding ratio of a fertilizer is adopted, so that nutrients required for the growth of the sweet corn are met; a classified sowing manner is adopted, so that the sweet corn is harvested by stages, and the harvested sweet corn appears on the market in a balanced manner; promotion of the growth of the sweet corn is facilitated, and resource waste is reduced; the sugariness of the sweet corn is improved; and corn stalk borer is controlled through carbofuran granules or BT emulsion, diseases and insect pests can be well controlled, and the growth of the sweet corn is not influenced neither.

The invention discloses compound seed coating agent and the preparation method thereof. The compound seed coating agent is manufactured with 5 to 10 kg of carbosulfan, 10 to 15kg of fernasan, 0.4 to 1kg of tebuconazole, 5 to 10kg of film former, 1 to 5kg of bentonite, 1 to 5kg of thickener, 1 to 5kg of emulsifier, 0.2 to 1kg of rhodamine, 1 to 5kg of antifreeze and 40 to 80kg of water. The invention has the characteristics of high efficiency and low toxicity. The toxicity of the invention is 20 to 30 times lower than that of the seed coating agent made from the carbofuran and thimet etc.; due to the mixture of the fernasan and tebuconazole, the sterilizing effect of the seed coating agent on the head smut in maize is 8 times higher than that of the single bactericide.

The invention discloses a single-purpose fertilizer of rice dry seedling, which is characterized by the following: allocating mass percent with 30-50% nitrogen fertilizer, 15-45% phosphate fertilizer, 10-32% potash fertilizer, 6-12% sulfur, 0. 01-0. 02% carbofuran and 0. 1-0. 2% multiple-effect azoles; choosing the nitrogen fertilizer as one or mixture from ammonia sulfate or ammonii chloridum; choosing the phosphate fertilizer as one or mixture from monoammonium phosphate, diammonium phosphate and super phosphate; choosing the potash fertilizer as potassium sulfate; setting the effective total nutrient at 25-50%; setting nitrogen (N) at 10-22%, phosphorus pentoxide (P2O5) at 7-20% and potassium oxide (K2O) at 5-16%. This invention possesses good soil adjusting acid function, which can prevent and cure major insect damage effectively.

The invention discloses a flue-cured tobacco slow release fertilizer and an application method thereof and solves problems that slow / controlled release fertilizers in prior art are high in production cost and complicated in technological process and membrane materials remained in the soil after fertilizer nutrients are released cause secondary pollution on soil environments. The the flue-cured tobacco slow release fertilizer is mainly composed of following components of, by weight, 8.48-9.78g of urea, 10.58-12.50g of monopotassium phosphate, 20.00-23.08g of potassium nitrate, 12.40-14.26g of potassium sulphate, 0.52-1.10g of cane sugar and 0.20-0.30g of carbofuran. According to the flue-cured tobacco slow release fertilizer and the application method thereof, the number of fertilizer applying can be reduced, labor intensity for applying the fertilizer is reduced, utilization efficiencies of the fertilizer are improved, environmental pollution is reduced and the flue-cured tobacco slow release fertilizer is low in production cost, simple in process, convenient to use and easy to popularize.

The invention discloses a novel maize seed dressing pesticide and fertilizer substrate and prescription and a preparation method thereof. The substrate comprises the components and contents according to weight: 80-95 portions of acetose attapulgite, 1-6 portions of farm-type neutral polyacrylic acid sodium salt, 2-8 portions of turves, 1-6 portions of multi-effect zinc fertilizer; each 100 portions of substrate is added with 0.05-0.5 portions of farm chemical carbofuran, 0.05-0.5 portions of carbendazim, 0.05-0.5 portions of thiram and / or 0.008-0.05 portions of paclobutrazol. The preparation method is that: the substrate materials are super-finely crushed into fine powder of more than 120 mesh, mixed according to ratio and prepared into the substrate; the farm chemical components are dissolved by the substrate into mother powder with concentration of 1-5 percent, the mother powder is incorporated into the substrate according to ratio, put into a double spiral mixer to be evenly mixed and stirred to get the finished products. The seed dressing pesticide and fertilizer produced through the substrate can be applied to maize seedling, form water retention and slowly releasing pesticide and fertilizer mud balls when the invention is used around the seeds and seedling roots and have a plurality of functions of keeping crop seedling from drought, strong seedling, ensuring strong root and seedling, disease and insects prevention, saving work and cost and increasing output and income, etc.

Provided is a Carbofuran monoclonal antibody hybridoma cell strain YH1 and an application thereof, which belongs to the field of food safety immunological detection. The Carbofuran monoclonal antibody hybridoma cell strain YH1 has been preserved in China General Microbiological Culture Collection Center, CGMCC for short, and the preservation number is CGMCC No. 12029. The monoclonal antibody secreted by the cell strain has better specificity and detection sensitivity on carbofuran, and IC50 value is 0.3 ng / mL, thus achieving detections of residual amounts of carbofuran in water, fruits and vegetables and cereals, providing conditions for immunodetection of residual carbofuran in food and having practical application value.

The invention provides a method for transplanting and rejuvenating hundred-year-old deciduous fruit trees. The method includes: selecting a hundred-year-old deciduous fruit tree growing up from a cutting or a seedling, cutting off every branch, excavating out soil covering a root portion along with the root portion for standby, spraying a thiophanate methyl solution, a transplantone solution and a brassins solution on the root portion, loosening soil at the transplant position, piling up a soil pile with loose soil, mixing carbofuran in the soil pile, placing a root of the hundred-year-old deciduous fruit tree on the soil pile, sealing the tree root with the soil, watering the root portion, and sinking the tree root to enable the soil pile to cover the tree root. The transplanted and rejuvenated hundred-year-old deciduous fruit trees are not only used for getting fruits, but also have viewing value and culture, are good scenery trees, and express old and vicissitude past and historical history charms; after being meticulously trimmed and formed, diverse shapes can be expressed, and since the rejuvenated old branches are provided with leaves, flowers and fruits, the transplanted and rejuvenated hundred-year-old deciduous fruit trees are delightful due to flowers in spring and fruits in autumn, and the effect for bringing out the crucial point is realized visually and in aspects of air purification and environment beautification.

The invention discloses a planting method of cyclocarya paliurus. The planting method comprises steps as follows: (1), land preparation, plowing and furrowing are performed from February to April, deep holes are dug in furrows, carbofuran granules are scattered, straw is laid and an organic fertilizer is applied to the holes sequentially, and then soil covering is performed; (2), shallow holes are dug at the deep holes one month after soil covering, tree seedlings are planted in the shallow holes, and roots of the tree seedlings are covered with pond mud before transplanting; (3), dichlorvos is sprayed 30-40 days after planting, plant ash is applied to the roots after spraying, dipterex is sprayed 2-3 months after planting, dichlorvos and dipterex are applied alternately every other 2-3 months, and a special fertilizer for the cyclocarya paliurus is applied 2-3 months after planting and applied once every six months. Lasting fertilizer efficiency is provided for quick growth of the tree seedlings in the manner of retaining fertilizer in the deep holes and planting the tree seedlings in the shallow holes, fertilization application and pest control measures taken after planting are scientific and reasonable, the tree seedlings grow fast, and a large quantity of leaves can be picked up next year.

The present invention relates to pesticide technology, and is recombinant acetylcholinesterase and its preparation process and usage in detecting pesticide residue. Recombinant acetylcholinesterase may be dissolved in buffering sodium phosphate solution and after being maintained at 4 deg.c for 22 days, it has 75 % of its initial enzyme activity maintained. It has inhibiting constant on DDVP, omethoate, paraoxon, methyl paraoxon, aldicarb, carbaryl, carbofuran and aphox of 4.4E6, 2.2E4, 1.1E6, 4.2E5, 4.8E4, 5.9E5, 5.1E6 and 2.3E5 separately. It is prepared through selecting seed, shaking cultivation, centrifuging, filtering, depositing, leaching, stirring, dialyzing and freeze drying, and is maintained at -20 deg.c. The detection process includes preparing reagent, treating sample, detecting sample, blank testing, testing sample, and final calculating. The recombinant acetylcholinesterase has obvious sensitivity on organophosphorus pesticide and carbamate chemicals and may be used in detecting pesticide residue.

The invention discloses a kind of enzyme linked immuno-sorbent assay reagent box applying to the analysis of the Carboruran residue, and it includes the box body, the 96-hole / 40-hole enzyme marker board placed in the box body and the reagent placed in the box body, and its characteristic lies in that in each hole of the enzyme marker board is the envelope antigen enveloped by the envelope liquid and able to combined and react with the anti-Carboruran antibody specioficity and sealed with 1.0-3.0% nonfat dried milk, and that the reagent in the box includes cleaning mixture, substrate-matter diluents, Carboruran standard solution, anti-CArboruran antibody, horseradish peroxide enzyme marker sheep anti-rabbit antibody, substrate matter, color-displaying matter and reaction-termiinated liquor.