12 results about "Procymidone" patented technology

The invention discloses a method for rapidly detecting pesticide residues in blueberries, and relates to the technical field of blueberry pesticide detection.The method comprises the steps that 10 g of a fully-ground blueberry sample is placed in a 100 ml centrifuge tube with a plug, 10 ml of a 0.1 mol / L hydrochloric acid aqueous solution and 10 ml of dichloromethane are added, 100 microliters of a mixed internal standard solution is added, after vortex oscillation is conducted for about 30 min, centrifugation is conducted for 5 min at the speed of 5000 r / min, 2 ml of a lower layer organic phase is taken, and the concentration of the lower layer organic phase is 10 ml; and after blow-drying by a nitrogen blowing instrument, adding 2ml of methanol for redissolving, and then adding an LCMS instrument for detection. The detection method disclosed by the invention can be used for simultaneously detecting 11 types of blueberry bactericides in national food standards and three types of bactericides such as mancozeb, metalaxyl and procymidone which are widely used in blueberry production at present. The result of the analysis method shows that the linearity of most components is good in the range of 5-100 g / L, the recovery rate of 14 pesticides is 94.9%-101.3% in the range of 5-50 [mu] g / kg, and the detection limit and the quantification limit of the method are 0.001 [mu] g / g-0. 03 [mu] g / g and 0.005 [mu] g / g-0. 010 [mu] g / g respectively. The method is simple, convenient and rapid to operate, and the sensitivity, accuracy and precision can meet the technical requirements of detection of various pesticide residues.

The invention belongs to the field of pesticide detection, and discloses an aptamer test strip for HCR and click chemistry combined signal amplification, which comprises a test strip assembly and an aptamer signal enrichment probe, a nucleic acid skeleton is self-assembled through HCR, and a high-density signal enrichment probe is formed by inducing cross-linking of gold azide nanoparticles through click chemistry reaction. According to the method, the detection limit of procymidone reaches 0.054 ng / mL, the color development response time is greatly shortened to be within 2 seconds, and the detection efficiency and sensitivity are remarkably improved. In actual vegetable sample detection, the recovery rate of the method is 93.44%-104.00%, and a specificity experiment shows that the method only responds to procymidone and is excellent in anti-interference capability. The detection capability of trace procymidone pesticide residues is remarkably improved, and a new idea of dual signal amplification is provided for the aptamer lateral flow chromatography technology.

The application relates to the field of biological medicines, in particular to application of dyclonine hydrochloride in preventing damage of a hypothalamus-pituitary-ovary axis of a female mouse. 2+ The application discloses a potential mechanism of combined procymidone damaging a hypothalamus-pituitary-ovary axis of a mouse, and provides a small-molecule drug, dyclonine hydrochloride, for preventing damage based on the mechanism. The configuration method of the small-molecule drug is as follows: dissolving dyclonine hydrochloride in a phosphate buffer, and then adjusting the pH value to about 7.4; the concentration for intraperitoneal injection is 10 mg / kg.bw / d. The small-molecule drug preparation method is simple, can effectively inhibit an RA signal path and an NF-kB inflammation path, is relatively safe, and can effectively protect the hypothalamus-pituitary-ovary axis of the female mouse.

The invention relates to the technical field of microorganisms, and particularly discloses lysinibacillus fusiformis KBD-5 for antagonizing botrytis cinerea and application of the lysinibacillus fusiformis KBD-5. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) Whole genome (accession number PRJNA1335659) sequencing reveals that the strain has a triple synergistic antagonistic mechanism for the first time: the strain contains a fengycin-like lipopeptide synthetic gene cluster to destroy pathogenic bacteria cell membranes, and contains GH18 chitinase and GH5 cellulase genes to degrade pathogenic bacteria cell walls. The inhibition rate of the strain on botrytis cinerea reaches 76.42%; the tomato fruit test morbidity is reduced to 25.9%, and the rotting index is reduced by 75.0%; the control effect of greenhouse potting reaches 72.35%, and the control effect is not obviously different from that of procymidone. Biological safety assessment shows that no toxicity factor exists, and no drug-resistant gene horizontal transfer risk exists. The invention further provides a microbial preparation containing the KBD-5 and application of the microbial preparation.

The invention discloses a preparation method and application of an antigen for detecting impurities such as etoxazole in vegetables and fruits. The obtained antibody is prepared into an enzyme linked immunosorbent assay kit, and when the enzyme linked immunosorbent assay kit is used for detecting etoxazole contained in vegetables and fruits, the detection limit is 10 [mu] g / L. The detection result is high in accuracy, especially in an etoxazole antibody specificity experiment, obvious specificity to etoxazole is shown, and the detection result is not interfered by drugs such as carbendazim, triazophos, chlorpyrifos, cyfluthrin, metalaxyl, acetamiprid, difenoconazole, procymidone, chlorfenapyr, profenofos and omethoate.

The application discloses a nucleic acid aptamer PCM-A09 which specifically recognizes procymidone, wherein the nucleotide sequence of the nucleic acid aptamer is shown as SEQ ID NO:1. The nucleic acid aptamer can specifically bind with procymidone. The nucleic acid aptamer PCM-A09 is single-stranded DNA (ssDNA) which is composed of 88 nucleotides. The secondary structure of the nucleic acid aptamer has outstanding stems and loops. The Gibbs free energy DG of the nucleic acid aptamer PCM-A09 is -16.14. The specificity and affinity of the nucleic acid aptamer are evaluated based on colloidal gold spectrophotometry. The experimental results show that the nucleic acid aptamer PCM-A09 can specifically bind with procymidone. The nucleic acid aptamer has the characteristics of high specificity and high affinity. The nucleic acid aptamer can be prepared into a PCM reagent, a detection kit or a sensor and applied to the detection of PCM in various agricultural products.

This invention discloses a strain of *Arthrobacter* (…). Pseudarthrobacter The strain F13, with accession number CCTCC NO:M 20241866 at the China Center for Type Culture Collection, was used in this invention to degrade procymidone. The results showed that the *Pseudomonas* F13 strain of this invention can efficiently degrade procymidone. This invention enriches the germplasm resources and diversity of microbial degrading strains and provides a new approach for the control of pesticide residue pollution.

The invention belongs to the technical field of prevention and control of bulbus fritillariae cirrhosae gray mold by algin, and particularly relates to a bulbus fritillariae cirrhosae gray mold ecological regulation and control pesticide decrement prevention and control method, in the early stage of bulbus fritillariae cirrhosae gray mold prevention and control, a trehalose chain vaccine organic water-soluble fertilizer diluent is uniformly sprayed to the whole field, and the trehalose chain vaccine organic water-soluble fertilizer contains more than or equal to 60g / L of organic matters and more than or equal to 60g / L of inorganic matters. The content of multi-element seaweed oligosaccharide is greater than or equal to 6.5%; according to the growth period of bulbus fritillariae cirrhosae and the occurrence condition of gray mold, biopesticide or chemical pesticide is jointly applied in stages, specifically, a bacillus subtilis preparation is applied in spring or autumn, and the content of effective components of the bacillus subtilis preparation is 10 billion spores / g; when the morbidity of the field gray mold is larger than or equal to 10%, chemical pesticide is applied, and the chemical pesticide is selected from one or two of 50% procymidone WP and 50% boscalid WDG to be alternately used.

The invention discloses a composite biological regulator based on a chitin derivative and a preparation method of the composite biological regulator. The composite biological regulator comprises the following active components: the chitin derivative, procymidone and valerian bacteria amine, the coating also comprises an auxiliary agent and water. Wherein the mass ratio content of the active ingredients in the composite biological regulator is 5.5-15%; the mass ratio content of the auxiliary agent in the composite biological regulator is 0.8-2.5%; the rest part of the composite biological regulator is the water; the chitin derivative is chitosan and / or chitosan oligosaccharide; according to the present invention, the chitin derivative is adopted as the main active component, and the component is derived from the natural plant, such that the good biodegradability is provided so as to reduce the environmental pollution caused by the chemical regulator. Meanwhile, procymidone and valerian bacteria amine are used as auxiliary active components and have a synergistic effect with the chitin derivative, so that the disease prevention and pest control effects of the regulator are enhanced, a relatively low dosage is kept, and potential risks to the environment and human bodies are reduced.

The invention belongs to the field of pesticide detection, and discloses a CHA and CRISPR combined mediated signal transduction and amplification aptamer test strip, which comprises the steps of test strip assembly and signal probe preparation, release of an initiation sequence after a target is combined with an aptamer to trigger CHA reaction to generate a pre-amplification signal, activation of a CRISPR / Cas12a system to cut a reporter molecule, and detection of the CHA and CRISPR combined mediated signal transduction and amplification. Therefore, transduction from an adapter signal to an immune signal and secondary amplification are realized, and Turn on type signal output is realized. In a specificity test, the method has no cross reaction on the structure and functional analogues of procymidone; and after being stored at room temperature for 28 days, the signal stability is kept at 84.61% or above. In actual vegetable sample detection, the recovery rate is 94.00%-104.20%. The trace detection capability of the research is obviously improved, and the Turn on type signal expression form is beneficial to visual interpretation of a detection result, so that a new strategy and reference are provided for development of an aptamer test strip of a small molecule target.

The invention discloses a pH tolerance photo-thermal composite material probe for detecting procymidone as well as a preparation method and application of the pH tolerance photo-thermal composite material probe. The pH tolerance photo-thermal composite material probe is obtained by coupling a quantum dot, prussian blue and covalent organic framework photo-thermal composite material with a procymidone nano antibody, the probe utilizes an ultraviolet laser to excite a material to generate a stable photo-thermal signal as a detection index for detection, has excellent pH tolerance, stability and photo-thermal performance, and can be used for qualitative and quantitative detection of procymidone. When the procymidone immunochromatography established based on the photothermal composite material probe is used for detecting procymidone, matrix interference in a sample can be eliminated by directly diluting the procymidone for 10-20 times after the procymidone is extracted by acetonitrile, so that the pretreatment step of the sample is greatly simplified; in actual vegetable sample detection, the recovery rate is 87.3%-111.0%, the detection result has high accuracy and repeatability, the procymidone recognition capability is high, the specificity is high, and the method has wide application prospects in the aspect of rapid detection of procymidone in complex samples.

The present application belongs to the technical field of pesticide detection, and particularly relates to a multi-in-one pesticide residue quantitative detection test paper and a detection method. The test paper is provided with gold nanoparticle labeled antibodies of at least two kinds of pesticides on a colloidal gold combination pad; a chromatography membrane is provided with a quality control line and at least two detection lines, each detection line is coated with an antigen of one kind of pesticide; the pesticides are selected from chlorpyrifos, phorate, dimethoate, acetamiprid, carbendazim, procymidone, fenpropathrin, carbofuran, isoprocarb, chlorothalonil, chlorfenapyr, parathion, tetramethylenedisulfide, triadimefon, isocarbophos or parathion. Through optimization of the pesticide types and preparation methods of the antigens and antibodies, the present application overcomes the problem of mutual interference of detection targets when multiple pesticides are simultaneously detected, has good specificity, high sensitivity, can accurately quantify, can simultaneously detect multiple components, and has simple pretreatment, and thus has good application prospect.