The invention relates to a bactericidal active compound composition, and especially relates to a composition comprising 2-mercaptobenzothiazole zinc and other bactericidal active compounds, and a preparation and an application thereof. The composition comprises a bactericidal active compound I and a bactericidal compound II. The bactericidal active compound I is 2-mercaptobenzothiazole zinc, and the bactericidal compound II is at least one selected from thiabendazole, procymidone, iprodione, propamocarb hydrochloride, carbendazim, thiophanate-methyl, validamycin, kasugamycin, tetramycin, Zhongshengmycin, oxadixyl, hymexazol, dimethomorph, flumorph, pyrimethanil, ethirimol, dithianon, metiram, thiram, zineb, mancozeb, propineb, fosetyl-aluminium, chlorothalonil, isoprothiolane, cymoxanil, fenaminosulf and copper hydroxide. The bactericidal composition provided by the invention has a significant synergistic effect, and can fully perform the controlling effects of 2-mercaptobenzothiazole zinc against bacterial, fungal and other diseases, and the good protective performance of 2-mercaptobenzothiazole zinc.