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470 results about "Food inspection" patented technology
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Machine vision-based real-time detection and grading method and machine vision-based real-time detection and grading device for pork appearance quality
InactiveCN101561402AHave objectivityAccuracyImage analysisMaterial analysis by optical meansMarbled meatPolarizer
The invention discloses a machine vision-based real-time detection and grading method and a machine vision-based real-time detection and grading device for pork appearance quality. The device comprises a hardware part for pork image acquisition and a software part for grading, wherein the hardware part for image acquisition consists of a camera, a lens, a polarizer, a light source, a computer, and the like. The method comprises the following steps: acquiring a pork image; carrying out real-time image processing and characteristic extraction through grading software; acquiring characteristic information reflecting the pork appearance quality; and evaluating the pork quality through a forecasting model, completing the grade estimation of color, marble vein, the tenderness and the comprehensive quality of underback, and completing the grade estimation of muscle color, the fat and the comprehensive quality of marbled meat. The method and the device can make the pork quality detection have objectivity, accuracy and high efficiency, and are suitable for the pork quality evaluation carried out by supermarkets, local food inspection authorities and import and export food inspection departments so as to standardize the pork market and consequently truly realize the pricing by the quality.
Owner:ZHEJIANG UNIV
Rapid parallel nucleic acid detection method and system based on micro-fluidic chip
ActiveCN104630373AMeet automatic sampling requirementsUniform heating temperature fieldBioreactor/fermenter combinationsSequential/parallel process reactionsFluorescenceDisplay device
The invention relates to a rapid parallel nucleic acid detection method and system based on a micro-fluidic chip. The nucleic acid detection system comprises a micro-fluidic chip, a motor, exciting light, a double-focal-plane imaging lens set, a detector, a signal acquisition processor and a display; the micro-fluidic chip comprises at least one reaction channel; a heating film is arranged at the periphery of the micro-fluidic chip; a submillimeter air layer is maintained between the micro-fluidic chip and the heating film; and the micro-fluidic chip is irradiated by adopting the exciting light, so that a nucleic acid sample generates fluorescence under excitation of the exciting light, the fluorescence is gathered on the detector by virtue of the double-focal-plane imaging lens set so as to generate an analog signal, the detector transmits the generated analog signal to the signal acquisition processor so as to generate a real-time fluorescence detection signal, and the real-time fluorescence detection signal is displayed by the display. The method and the system disclosed by the invention can be applied to the fields of clinical pathogenic bacterium molecular diagnosis, food inspection and quarantine, food poisoning pathogenic bacterium detection, bacteriology classification and epidemiological investigation and have huge economical and social benefits.
Owner:CAPITALBIO CORP +2
Method for analyzing and detecting a plurality of endocrine disruptors in food
ActiveCN103185762AHigh sensitivityAccurate analysisComponent separationPerturbateurs endocriniensLiquid milk
The invention belongs to the field of food inspection, relates to an assay determination method for endocrine disruptors in food, and particularly relates to a method for analyzing and determining a plurality of endocrine disruptors in milk powder and liquid milk. The method comprises the steps of dissolving a sample with water, adding an organic solvent mixable with water, extracting a to-be-detected material by ultrasonic, adding a sodium salt to make the organic solvent separated from a water phase to realize liquid-liquid extraction, taking an organic solvent containing quantitative to-be-detected material, purifying by extraction with a solid phase filled with C 18 materials, deriving by dansyl chloride, and analyzing and detecting four types of 26 endocrine disruptors in the food by using ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry at the same time. The method can overcome the disadvantages that a conventional technology cannot realize simultaneous analysis of the plurality of the endocrine disruptors in the food by once chromatographic sample injection, can increase sensitivity of the quadrupole-time of the flight-mass spectrometry, and realize accurate analysis of the endocrine disruptors in the food by using the quadrupole-time of the flight-mass spectrometry.
Owner:FUDAN UNIV
Adjustable food inspection working table
The invention discloses an adjustable food inspection workbench, which comprises a bottom plate, a supporting leg, a rotating shaft, a motor, a first internal thread sleeve, a first connecting rod, a second connecting rod, a side plate, a double-axis motor, and a threaded rod , the second internally threaded sleeve, the third connecting rod, slide rails, sliders, universal rotating shaft and test tube rack, the present invention is provided with a motor, a rotating shaft, the first internally threaded sleeve, the first connecting rod and the second connecting rod The rod, through the cooperation of the above parts, makes the roller contact or separate from the ground, which is convenient for the movement of the workbench. It is equipped with a double-axis motor, a threaded rod and a third connecting rod. The height of the workbench body can be adjusted through the cooperation of the above parts, which is convenient for different heights. It is equipped with slide rails, sliders and universal rotating shafts, and the support rod is pulled to make the sliders slide along the slide rails, which is convenient for the staff to take and place the test tubes on the test tube rack. The rotating shaft can realize the adjustment of the angle of the test tube rack.
Owner:深圳市华帮国际商务服务有限公司
Contaminant Detector For Food Inspection
InactiveUS20080118026A1Low costLow production costRadiation/particle handlingX-ray tube electrodesX-rayDetector array
A contaminant detection machine (1) including a conveyor (3) which causes an object under inspection (79) to pass through a plane (48) of emitted x-ray radiation. The plane is generated by an x-ray tube (55) that emits a lateral beam, thereby permitting the distance (88) between the x-ray tube and the object under inspection to be reduced. A photo diode arch mounting assembly (104) is placed above the object under inspection and is mated to a collimator assembly (125) that also serves as the mounting bracket for the x-ray generation assembly (38), thereby preserving optical alignment between the photo diode detector array (28) and the emitted x-ray plane (48). The detector array (28) scans the object under inspection (79) so as to produce a continuous series of discrete lines, each line being analyzed by an image processing unit (116) to determine the presence or absence of a contaminant. The conveyor (3) passes over a pair of slider bed surfaces (155, 156) which are mounted in a hinged manner such that the leading edge (168) of one surface (156) is parallel to and spaced apart from the trailing edge (172) of the other surface (155), thereby creating a gap that is coplanar with the collimation slot (129) and the emitted x-ray plane (48). Each bed surface (155, 156) is rigidly constrained within open ended mounting brackets (159, 160, 161 and 162) yet can be removed by hand without the use of tools. Similarly, the conveyor (3) is supported by a roller assembly (182) that includes a tracking block (142) and pivot pin (143) which permits the roller assembly to be mounted to and removed from flip up mounts (151, 152) by hand and without the need of tools. Graphical user interfaces (249, 260, 261, 266, 275, 282 and 288) permit a user to operate the machine (1) by means of a liquid crystal display touch screen (20).
Owner:THERMO FISHER SCIENTIFIC INC
Glycine diagnosis/measuring reagent kit and glycine concentration determination method
InactiveCN101464279AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsGlycine2-ketoglutaric acid
The invention relates to a kit for diagnosing / measuring glycine by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the glycine, and belongs to the technical field of medical / food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, a 2-ketoglutaric acid, glycine aminotransferase, glutamate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the glycine.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Method for determining galactose concentration and galactose diagnosis/determination reagent kit
InactiveCN101324580AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsELISA unitLactose
The invention relates to a kit for diagnosing / mensurating galactose by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA). The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the galactose, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, galactose oxidase, NADH peroxidase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the galactose.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Food inspection and sampling device
ActiveCN106053134AImprove securityMeet needsWithdrawing sample devicesLavatory sanitoryFood safetyUv disinfection
The invention discloses a food inspection and sampling device, which comprises a body, a food placing groove, a guide rail, a smell sensor and a conveyor belt. A display screen and a control panel are inlaid in the outer wall of the body. The display screen is installed above the control panel. A handle is fixed at the upper left side of the control panel. The food placing groove is welted and fixed on the outer wall at the right side of the body, and a discharge door is installed below the food placing groove. A plug wire receiving room is arranged at the lower right side of the discharge door. The bottom of the body is provided with rollers. The guide rail is installed on the inner wall of the body. A slide block is fixed on the top of a telescoping rod, and the smell sensor is installed below the telescoping rod. As an ultraviolet sterilizer is arranged inside a workroom, the workroom can be sterilized at real time, and the whole sampling process is completed in an aseptic and nontoxic environment. Thus, food safety is raised, and requirements of people are met.
Owner:HAINING IDIOU ANIMAL HEALTH PROD TECH CO LTD
Indirect competition enzyme linked immunoreagent kit for detecting lead ions and manufacturing method thereof
InactiveCN103472230AResolving the immunogen
<u>sex</u>
questionHigh potencyMaterial analysisAbzymeAntigen
The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting lead ions and a manufacturing method of the kit. An elisa plate enveloped by lead ion envelope antigens, lead-ion monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a lead ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The indirect competition enzyme linked immunoreagent kit can detect the lead irons in a trace mode, is used for detecting the lead iron contamination residual in the environment, the soil, water, foods and containers, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit not only can be used for screening samples in large batch, but also can perform rapid detection on samples in small batch, and provides effective means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.
Owner:HENAN INST OF SCI & TECH
Inspection method and device of food harmful substances
PendingCN109406508AAvoid the impact of test resultsCrushed fullyMaterial analysis by observing effect on chemical indicatorHazardous substanceEngineering
The invention relates to the technical field of food inspection, and discloses an inspection method and device of food harmful substances. The device comprises a base, a sliding groove is formed in the top of the base, a pulley is movably installed in the sliding groove, the top of the pulley penetrates through the sliding groove and extends to the outer side of the sliding groove to be fixedly connected with a bottom plate, an inspection instrument is fixedly installed on the top of the bottom plate, a waste liquid tank is fixedly installed on the top of the bottom plate, a side plate is fixedly installed on the top of the base, and a first supporting seat is fixedly installed on the right side face of the side plate. According to the method and the device, a sample can be allowed to be fully pulverized through a pulverizing device, so that the harmful substances flow and mix with buffering liquid as much as possible, an inspection result is more accurate, a cover plat can be opened to clean a pulverizing barrel, a second filter net can be taken out to be cleaned, and therefore the influence on the next inspection result caused by residues is prevented.
Owner:杭州和宇食品有限公司
Surface-enhanced raman scattering base on basis of special-material superabsorbers and preparation method thereof
InactiveCN104764732AWide Excitation Wavelength RangeEnhanced Raman signalRaman scatteringPhotonicsPhysical chemistry
The invention belongs to the technical field of micro-nano photonics and bioanalytical chemistry and particularly relates to a surface-enhanced raman scattering base on the basis of special-material superabsorbers and a preparation method thereof. The surface-enhanced raman scattering base is an optical special-material superabsorber with a three-layer structure on a substrate, and the three-layer structure comprises a continuous metal film layer, a medium isolating layer and a discontinuous metal film layer; the discontinuous metal film layer is composed of metal particle arrays at nano feature size and mutual separated. The base is prepared through a film-plating and heat-treatment technology, and a coupling micronano antenna having controllable overall shape and appearance and taking the metal particle arrays as the top layer is prepared at low cost in a large area, so that ultra-wide spectrum optical absorbing performance is realized. The preparation method has the advantages of simple operation, good repeatability and low cost. The surface-enhanced raman scattering substrate has the very important application to the fields of food inspection, environmental supervision, medical detection and chemical and biological analysis and the like.
Owner:FUDAN UNIV
Indirect competition enzyme linked immunoreagent kit for detecting mercury ions and manufacturing method thereof
The invention relates to an indirect competition enzyme linked immunoreagent kit for detecting mercury ions and a manufacturing method of the kit. An elisa plate enveloped by mercury ion envelope antigens, mercury-ion-resisting monoclonal antibodies, elisa second antibodies, a substrate color developing solution, a stop solution, a mercury ion standard solution, a washing liquor concentrated solution and a sample treating liquid are arranged in the kit. The kit can detect the mercury irons in a trace mode, is used for detecting the mercury irons in the environment, the soil, water, foods, medicines, cosmetics and the like, and has the advantages of being rapid, easy and convenient to use, sensitive, peculiar, economical and the like. The kit is few in detection step and high in timeliness, saves detection time, reduces operation errors and can perform field detection. The kit is low in requirement for pretreating samples, simple in treatment process, not only can be used for screening the samples in large batch, but also can perform rapid detection on the samples in small batch, and not only provides technical support for environment and food safety, but also provides effective technological means for food import and export inspection, food inspection, monitoring and evaluation of environmental pollution and the like.
Owner:ZHENGZHOU UNIV
Rapid sensitive method for detection of freshness of meat
InactiveCN104458888AEasy to detectImprove safety indexMaterial analysis by electric/magnetic meansQuarantineIon-mobility spectrometry
The invention discloses a rapid sensitive method for detection of the freshness of meat. The method uses an ion mobility spectrometry detection technology as the basic technology, and employs positive and / or negative ion mode for the establishment of an ion mobility spectrometry method for the rapid detection of the freshness of meat. Under the experimental conditions, the changes of released components and content variation of the same component in the spoilage process of meat can be monitored continuously. A detection device provided by the invention has the advanategs of high sensitivity, easy assembly, low cost, easy popularization, and suitability for on-site rapid detection and mass screening. The method can rapidly and sensitively detect freshness of meat and increase the efficiency and accuracy of food inspection and quarantine.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Surface plasma body resonant vibration and flexible flat plate wave combined detection biologic sensor
InactiveCN101109747AEnsure consistencyGuaranteed synchronicityMaterial analysis using sonic/ultrasonic/infrasonic wavesScattering properties measurementsAcoustic wavePlasma resonance
The invention relates to a biologic detecting sensor combining surface plasma resonance with flexible plate wave that is applicable for detecting the biologic affinity reaction feature in such fields as clinic inspection, medicine screening, environment and food inspection, etc., which comprises an optic sensing component and a sound wave sensor, between which a sample pond for bearing the object to be detected is arranged. By the biologic sensor, a plurality of parameters of the object can be measured in the same test condition, and the accuracy of the physical characterization of the biologic reacting object can be greatly improved.
Owner:CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Glycine diagnosis/measuring reagent kit and glycine concentration determination method
InactiveCN101464271AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsGlycineAbsorbance
The invention relates to a kit for diagnosing / measuring glycine by utilizing the technology of the enzymic colorimetry. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the glycine, and belongs to the technical field of medical / food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, glycine dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the glycine.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Glycine diagnosis/measuring reagent kit and glycine concentration determination method
InactiveCN101464280AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurements2-ketoglutaric acidEnzymatic Colorimetry
The invention relates to a kit for diagnosing / measuring glycine by utilizing the technologies of the enzymatic doubling amplification method, the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the glycine, and belongs to the technical field of medical / food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, a 2-ketoglutaric acid, guanosine monophosphate, glycine aminotransferase, glutamate dehydrogenase, guanosine monophosphate reduced enzyme and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the glycine.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Detecting biologic sensor associating evanescent field and flexible flat plate wave
InactiveCN101109748AEnsure consistencyGuaranteed synchronicityMaterial analysis using sonic/ultrasonic/infrasonic wavesScattering properties measurementsAcoustic waveOptical sensing
The invention relates to a biologic detecting sensor combining evanescent field with flexible plate wave that is applicable for detecting the biologic affinity reaction feature in such fields as clinic inspection, medicine screening, environment and food inspection, etc., which comprises an optic sensing component and a sound wave sensor, between which a sample pond for bearing the object to be detected is arranged. By the biologic sensor, a plurality of parameters of the object can be measured in the same test condition, and the accuracy of the physical characterization of the biologic reacting object can be greatly improved.
Owner:CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Contaminant detector for food inspection
InactiveUS7450686B2Low costFree of ChargeRadiation/particle handlingX-ray tube electrodesDetector arrayImage manipulation
Owner:THERMO FISHER SCIENTIFIC INC
Portable fruit and vegetable food inspection apparatus
ActiveCN109358168AGuaranteed accuracySimple structurePreparing sample for investigationTesting foodLiquid storage tankEngineering
The invention discloses a portable fruit and vegetable food inspection apparatus. The apparatus comprises: a housing; a sampling head comprising a first portion, a second portion, and a third portion;a crushing member comprising a second gear plate and a plurality of second blades; a soaking tank; a first blocking plate; a material guide tank; a second blocking plate; a first inspection tank; a second inspection tank; a recovery tank; a liquid storage tank; and a second rotating shaft. According to the portable fruit and vegetable food inspection apparatus, food sampling, sample pretreatmentand sample inspection are integrated. The food can be inspected in the field, and the food sample can be inspected multiple times. The whole inspection process is carried out inside the housing without touching an inspection sample and a test paper by hand, thereby proving a sterile inspection environment, avoiding the influence on an inspection result by external contact, and ensuring the accuracy of the inspection result.
Owner:方贵雄
Method for integrative detection of false packed dairy products in qualitative and quantitative modes
InactiveCN105136953AQuantitative detection of adulterationValid identificationComponent separationMaterial analysis by electric/magnetic meansProtein detectionOrganism
The invention relates to the bioanalysis technology and the food inspection field and provides a method for integrative detection of false packed dairy products in qualitative and quantitative modes. The method is a complete technical scheme which integrates sodium dodecyl sulfate-polyacrylamide gel electrophoresis, liquid chromatography-tandem mass spectrometry and moving reaction boundary electrophoresis titration, and particularly is a complete technical route from qualitative identification on whether false packed dairy products exist, identification of the kind of false protein to quantitative analysis of the ratio of false protein. Compared with existing detection methods, the method has the advantages that whether false packed dairy products exist can be effectively identified, the kind of false protein can be identified in a qualitative mode, and false degree can be measured in a quantitative mode; beside diary product inspection, the method can also be used for inspection of other protein food from qualitative identification to quantitative analysis. Compared with a traditional protein detection method, the method has the advantage that interference of non-protein nitrogen can be avoided.
Owner:SHANGHAI JIAO TONG UNIV
Evanescent wave optical fiber biosensor and use thereof
InactiveCN101393202ARealize quantitative detectionImprove stabilityBiological testingFluorescence/phosphorescenceBiological targetFood inspection
The invention relates to a biological detection device capable of being widely applied in fields such as molecular biology, biomedicine, food inspection, biological agents, environment monitoring and so on, in particular to an evanescent wave-based optical fiber biosensor capable of realizing quantitative and accurate detection to a biological target. The evanescent wave-based optical fiber biosensor comprises an optical detection system and an optical fiber bio-probe consisting of an optical fiber and capture biomolecules implanted on the surface of the optical fiber, wherein internal calibration ions of doped La elements or transition elements are contained in the body of the optical fiber, and the concentration of the internal calibration ions is between 500 and 4000ppm. The evanescent wave-based optical fiber biosensor realizes the quantitative detection of internal calibration reference in the internal calibration reference, simplifies the complex process that optical fiber biosensors for reference and detection are adopted to synchronously work in a semi-quantitative or quasi-quantitative biological detection technology of the prior art, and improves the stability of quantitative detection values and detection accuracy.
Owner:CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Glycine diagnosis/measuring reagent kit and glycine concentration determination method
InactiveCN101464272AFast measurementImprove accuracyMicrobiological testing/measurementColor/spectral properties measurementsGlycineEnzyme catalysis
The invention relates to a kit for diagnosing / measuring glycine by utilizing the technologies of the doubling amplification method, the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the glycine, and belongs to the technical field of medical / food inspection and measurement. The main components of the kit include a buffer solution, coenzyme, inosinic monophosphate, glycine dehydrogenase, guanosine monophosphate reduced enzyme and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the increase in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the active concentration of the glycine.
Owner:SUZHOU ANJ BIOTECHNOLOGY CO LTD
Method for detecting concentration of protein in liquid
InactiveCN102539420AHigh sensitivityLess intrusiveChemiluminescene/bioluminescenceDecompositionPeroxidase
The invention discloses a method for detecting concentration of protein in a liquid, and belongs to the technical field of food inspection and detection. The method comprises the following steps that: the protein in a liquid sample is combined with ferric porphyrin to form a compound with a peroxidase function, the compound catalyzes hydrogen peroxide (H2O2) to generate active oxygen, and the active oxygen oxidizes luminol to form an organic peroxide; the peroxide is instable, nitrogen is immediately obtained through decomposition, and excited state amino-o-phthalic acid is generated; and during conversion from an excited state to a ground state, the released energy is detected in a photon form, and the quantity of the protein in the liquid sample to be detected can be calculated by detecting the change of the number of photons before and after reaction. The invention has the advantages that: the method has the characteristics of high sensitivity, low interference, economy, practicability and the like, and can be widely applied to the field of food safety monitoring in medicine health and food manufacturers, farmer's markets, quality supervision and epidemic prevention departments and the like.
Owner:侯巍 +3
Immunomagnetic bead containing OmpU antibody, preparation of immunomagnetic bead and method for capturing pathogenic vibrios and detecting pathogenic vibrios through multiplex PCR by utilizing immunomagentic bead
InactiveCN105859883AQuick checkFast, accurate and efficient detectionMicrobiological testing/measurementImmunoglobulins against bacteriaAntigenMultiplex pcrs
The invention relates to an immunomagnetic bead containing an OmpU antibody, preparation of the immunomagnetic bead and a method for capturing pathogenic vibrios and detecting the pathogenic vibrios through multiplex PCR by utilizing the immunomagnetic bead. PCR amplification of an OmpU gene is conducted by taking genomes of the vibrios as templates, pure OmpU recombinant protein is obtained to serve as an antigen to prepare the antibody, and the immunomagnetic bead containing the OmpU antibody is prepared through the coupling-washing process; the immunomagnetic bead can capture multiple vibrios, and then the vibrios are identified by multiplex PCR specific primers which are designed on the basis of a danJ gene. The immuomagnetic bead can simultaneously detect the vibrio parahaemolyticus, the vibrio cholera, the vibrio alginolyticus, the vibrio vulnificus and the vibrio mimicus at most and can be widely applied to environment and import and export food inspection. The immuomagnetic bead combines the specificity of immune separation and the high efficiency of PCR amplification, has the capacities of separation, concentration and enrichment, can efficiently separate and enrich the low-concentration pathogenic bacteria in a sample, reduces or eliminates inhibition and influences of the sample to PCR, improves detection limitation and has the very good application prospect.
Owner:SHANTOU UNIV
Signal characterization method of visual array sensor
ActiveCN104880422AEasy accessImprove detection stabilityMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsLength waveComputer science
The invention discloses a signal characterization method of a visual array sensor. The full spectrum information of the visual array sensor is obtained by use of a hyperspectral imaging technology, and a spectrum difference value under a characteristic wavelength is extracted from the full spectrum information and applied to the signal characterization of the visual array sensor. The signal characterization method is capable of improving the detection accuracy and the stability of the visual array sensor and can be applied to the fields of multi-component gas detection, food inspection and the like.
Owner:JIANGSU UNIV
Specific nucleic acid aptamer for recognizing streptomycin and application of nucleic acid aptamer to streptomycin detection
ActiveCN104745588AStrong specificityHigh affinityBiological testingDNA/RNA fragmentationNucleic acid detectionNucleotide
The invention discloses a specific nucleic acid aptamer for recognizing streptomycin. The nucleotide sequence of the aptamer is shown as SEQ ID NO: 1. Meanwhile, the invention further discloses a method for qualitatively and quantitatively determining streptomycin by using the nucleic acid aptamer and application of the nucleic acid aptamer to food inspection. According to the invention, streptomycin is taken as a target substance, and a high-affinity and high-specificity nucleic acid aptamer for recognizing streptomycin is screened by an SELEX technology through affinity chromatography and is combined with colloidal gold colorimetry, so that a novel streptomycin detection method based on the aptamer is built. The method can be used for qualitative or quantitative detection of streptomycin simply, quickly, accurately, economically and specifically, and can be widely applied to the fields of food, sanitation, import and export inspection and quarantine, and the like.
Owner:HEBEI UNIVERSITY
Hericium erinaceus nondestructive test grading method based on machine vision
ActiveCN107564000AQuick checkAccurate detectionImage analysisCharacter and pattern recognitionPattern recognitionMachine vision
The invention relates to a hericium erinaceus nondestructive test grading method and belongs to the technical field of food inspection. The method includes steps of: extracting a characteristic parameter from a Hericium erinaceus image, establishing a corresponding discrimination function, and performing hericium erinaceus nondestructive test grading. Compared with artificial grading, the method can not only realize rapid and accurate Hericium erinaceus detection, but also completely avoids damage, improves and quantifies an existing grading index, namely, takes three good indexes including ofthe color, the size and the shape of the Hericium erinaceus, as grading indexes of the Hericium erinaceus, and provides a scientific grading standard for Hericium erinaceus industrialized productionprocess.
Owner:NANJING XIAOZHUANG UNIV
Method for detecting horsemeat in food
ActiveCN104293899AQuick checkSensitive detectionMicrobiological testing/measurementDNA/RNA fragmentationFluorescenceAdditive ingredient
The invention relates to a method for detecting horsemeat in food. The invention provides a primers for detecting 6 pairs of different fragments horsemeat in food, and the nucleotide sequences are shown as SEQ ID NO.1, NO.2, NO.3, NO.4, NO.5, NO.6, NO.7, NO.8, NO.9, NO.10, NO.11 and NO.12. The invention also provides a kit containing the above primer group. The kit of the invention can fast and accurately detect whether a meat product is doped with horsemeat, and has especially obvious effect in detection of depth processing meat. The invention also provides flexible changeable objective fragments for food inspection personnel to contrast and detect other meat sources, thereby improving the efficiency of detection. In addition, the primers provided by the invention can also be used as primers for real-time fluorescence quantitative PCR to conduct quantification on the horsemeat ingredient doped in food, for better application in food field.
Owner:神州基因科技(天津)有限公司
Method for rapidly detecting edible oil adulterated with frying oil by utilizing three-dimensional fluorescence spectrum technology
InactiveCN109115744ALow costChemiluminescene/bioluminescenceMaterial testing goodsVegetable oilRapeseed
The invention belongs to the technical field of food inspection, and discloses a method for rapidly detecting edible oil adulterated with frying oil by utilizing a three-dimensional fluorescence spectrum technology. According to an actual possible proportion, common vegetable oils, such as peanut oil, sesame oil, soybean oil, corn oil and rapeseed oil, are mixed with frying oil repetitively used at high temperature; and 3mL of adulterated oil is put into a quartz cuvette and tested in a three-dimensional fluorescence detection software of a PerkinElmer fluorescence / phosphorescence / chemiluminiscence photometer, so that a corresponding spectrogram is obtained. Spectrograms of pure vegetable oils are compared with the adulterated spectrogram, differences between the spectrograms are found out, and a conclusion is drawn. According to the method, vegetable oils and frying oil are mixed on the basis of the actual possible proportion, 3mL of adulterated oil is put into the quartz cuvette andtested in the PerkinElmer fluorescence / phosphorescence / chemiluminiscence photometer, and ultimately, the corresponding spectrogram is obtained. The invention establishes a simple, rapid and low-cost detection method for identifying edible oil adulteration.
Owner:SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
High performance liquid chromatography-tandem mass spectrometry detection method of plasticizer in sugar
ActiveCN103995075ASimplify detection stepsRealize detectionComponent separationRetention timePlasticizer
The invention discloses a high performance liquid chromatography-tandem mass spectrometry detection method of a plasticizer in sugar, comprising the following steps: (1) preparing a standard solution and detecting by high performance liquid chromatography-tandem mass spectrometry to obtain retention time and standard spectrum diagram; (2) making relationship curves between concentration and chromatographic peak area according to varieties of phthalates from the standard spectrum diagram; (3) carrying out pretreatment of a sugar sample and detecting by high performance liquid chromatography-tandem mass spectrometry to obtain a sample spectrum diagram; and (4) comparing a detection result obtained from the step (3) with the standard curve obtained from the step (2), and calculating to determine the content of the plasticizer in sugar. The method provided by the invention has advantages of simple detection step and fast detection time. A simple and feasible detection method of a plasticizer can be provided for a food production enterprise or food inspection institution. Detection of the content of a plasticizer in sugar can be realized. In particular, high sensitivity detection of DINP can be realized.
Owner:广东省科学院生物与医学工程研究所
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