235 results about "Perturbateurs endocriniens" patented technology

The invention belongs to the field of food inspection, relates to an assay determination method for endocrine disruptors in food, and particularly relates to a method for analyzing and determining a plurality of endocrine disruptors in milk powder and liquid milk. The method comprises the steps of dissolving a sample with water, adding an organic solvent mixable with water, extracting a to-be-detected material by ultrasonic, adding a sodium salt to make the organic solvent separated from a water phase to realize liquid-liquid extraction, taking an organic solvent containing quantitative to-be-detected material, purifying by extraction with a solid phase filled with C 18 materials, deriving by dansyl chloride, and analyzing and detecting four types of 26 endocrine disruptors in the food by using ultra-high performance liquid chromatography-quadrupole-time of flight-mass spectrometry at the same time. The method can overcome the disadvantages that a conventional technology cannot realize simultaneous analysis of the plurality of the endocrine disruptors in the food by once chromatographic sample injection, can increase sensitivity of the quadrupole-time of the flight-mass spectrometry, and realize accurate analysis of the endocrine disruptors in the food by using the quadrupole-time of the flight-mass spectrometry.

A polycarbonate resin reduced in the release of environmental hormones which comprises structural units represented by general formula (A) and structural units represented by general formula (B), the structural units represented by general formula (A) accounting for 5 to 90 mol % of all the structural units, and which has an intrinsic viscosity of 0.30 to 0.60 dl/g. The polycarbonate resin is produced through the copolymerization of a bispenol whose ability to link to estrogen acceptors is low with an aliphatic dihydroxy compound. The resin hence has the basic properties inherent in polycarbonates, i.e. mechanical strength and heat resistance, and is reduced in the release of environmental hormones.

The invention relates to a detection technology of endocrine disrupters in water environment, in particular relating to a method for detecting seven substances such as oestrone, 17 beta-estradiol, estriol, 17 alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A and the like in a water environment sample by virtue of liquid chromatogram-tandem mass spectrometry. The method comprises the following steps: concentrating and gathering the estrogen, the nonyl phenol, the octylphenol and the bisphenol A in a water sample by utilizing a solid phase extraction column; eluting the estrogen, the nonyl phenol, the octylphenol and the bisphenol A from the solid phase extraction column by utilizing ethyl acetate; and analyzing the concentrations of the estrogen, the nonyl phenol, the octylphenol and the bisphenol A by virtue of liquid chromatogram-tandem mass spectrometry. The method has the advantages of environment friendliness and easiness for operation, ensures relatively high recovery, and can be used for fast analyzing the concentrations of the estrogen, nonyl phenol, octylphenol and bisphenol A in the water sample.

The invention discloses a photoelectric chemical analysis method adopting a molecular imprinting functionalization modified electrode, relates to a molecular imprinting technology-based preparation method of a high-selectivity TiO2NTs modified electrode, and also relates to a method for high-selectivity and high-sensitivity detection of endocrine disrupting chemicals especially such as 2,4-dichlorophenoxyacetic acid (2,4-D) having no electrochemical activity by the high-selectivity TiO2NTs modified electrode as a photo anode. Compared with the prior art, the molecular imprinting technology-based preparation method creatively utilizes a molecularly imprinted polymer in selective modification of a photoelectric sensing electrode so that selectivity and detection sensitivity of an electrode are improved greatly; the interference caused by multiple concomitant micro-molecules is eliminated; a magnitude of a detection limit reaches to 10<-8>mol/L<-1>; the molecular imprinting functionalization modified electrode is stable; and the photoelectric chemical analysis method for detection has good reappearance.

The invention provides an endocrine interferent molecularly imprinted membrane substrate in a trace environment with rapid detection speed and high sensitivity, the preparation method and the application thereof. The molecularly imprinted membrane substrate comprises a gold quartz crystal substrate, and multi-layer nano-materials that alternate with each other and environmental endocrine interferent molecularly imprinted polymer are formed on the surface of the gold quartz crystal substrate. The preparation method comprises the following steps: selecting functional monomer that can be synthesized with the environmental endocrine interferent into the molecularly imprinted polymer; preparing the molecularly imprinted polymer solution; and modifying the nano-material and the molecularly imprinted polymer on the surface of the gold quartz crystal substrate. The invention applies the surface modification technology to the preparation of the molecularly imprinted membrane substrate, so as to allow the controllability over the preparation of the nano-scale synergetic EDCs molecularly imprinted membrane substrate. A method for detecting the environmental endocrine is rapid and simple, and the reaction and the result are automatically recorded and completed by an instrument with good repeatability, thereby facilitating the on-site detection.

The invention discloses a molecular-dynamics-simulation-based virtual screening method of nuclear receptor mediated endocrine disruption substances, and belongs to the field of virtual screening and activity prediction of environmental suspicious endocrine disruption substances. The molecular-dynamics-simulation-based virtual screening method includes the steps of carrying out butt joint on a receptor file obtained by the fact that optimization and testing or homology modeling are/is carried out on tested small molecules to form a compound, and then using a GROMACS software package to carry out molecular dynamics simulation. Motion trail analysis is carried out on a twelfth helix of a receptor, pollutants with the activity of the receptor are identified through a changing curve of root-mean-square deviation analysis of a space position along with time changes, within the required time, a corresponding helix is regarded to be located to a determined position when the curve tends to be stable, and the receptor has the biological activity. In addition, the located position is inspected to judge whether the activity is fitting or resistance.

The invention relates to a detecting technique of endocrine disrupters in water environment and particularly relates to a technique for quantitative analysis on oestrone, 17beta-estradiol, estriol, 17alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A in a complex substrate water sample by adopting a liquid chromatogram-tandem mass spectrum combined technique. The method comprises the following steps: enriching a collected water sample by using a HLB (Hydrophile-Lipophile Balance) solid-phase extraction column and washing with carbinol; drying elution liquid nitrogen and purifying by using Florisil; and finally, analyzing by utilizing the liquid chromatogram-tandem mass spectrum combined technique. The method is environment-friendly, easy to operate and high in recovery rate. The method can quickly analyze the trace amount of oestrone, 17beta-estradiol, estriol, 17alpha-ethinyl estradiol, nonyl phenol, octylphenol and bisphenol A in the complex substrate water sample.

The present invention provides an aptamer capable of specifically adsorbing to bisphenol A suspected to be an endocrine disrupter as a target molecule, a method for obtaining an aptamer capable of specifically adsorbing to bisphenol A by an in vitro selection method utilizing affinity chromatography using a carrier immobilizing bisphenol A, particularly, a method including use of an antagonistic elution buffer containing an amphiprotic organic solvent for elution by the affinity chromatography, and a single-strand nucleic acid molecule which is an aptamer obtained thereby.

A baby bottle for preventing the release of endocrine disrupters is disclosed. The baby bottle includes a glass vessel containing milk. A protective cover has shock absorbing members to protect the glass vessel, and includes left and right cover bodies having a fastening protrusion and a fastening hole, respectively. An air inlet packing is placed on the upper end of the glass vessel to control inflow of external air, and is coupled to a ventilation tube and a nipple. A support cap is fastened to the upper end of the protective cover in such a way that the nipple is in close contact with the upper portion of the air inlet packing, when the air inlet packing is in close contact with the glass vessel. Thus, the baby bottle protects a baby from endocrine disrupters, and prevents air from flowing backwards during feeding, thus preventing the baby from spitting

The invention provides the preparation of a molecularly imprinted hybrid material of an estrogenic endocrine disrupter. The preparation comprises the following steps: firstly, loading Fe3O4 microspheres on the surface of graphene by a hydrothermal one-step method to obtain magnetic graphene, and then taking the magnetic graphene as a carrier, and synthesizing a magnetic graphene-based molecularly imprinted nanofilm on the surface of the magnetic graphene through the molecular imprinting technique. The preparation of the molecularly imprinted hybrid material provided by the invention is rational in process and easy to implement; the magnetically functionalized graphene in the prepared magnetic graphene-based molecularly imprinted hybrid material is magnetic and thus convenient to separate, and simultaneously, capable of enhancing the response signals of an electrochemical sensor; the molecularly imprinted hybrid material has the characteristics of excellent chemical stability and reusability, quick response and high sensitivity in detection of estrogen in drinking water; therefore, the molecularly imprinted hybrid material is greatly convenient for the detection of the estrogenic endocrine disrupters; as a result, the utilization efficiency of the molecular imprinting technique can be greatly improved and the range of molecular imprinting application can be expanded.

The invention discloses a scale advanced treatment method for livestock and poultry farming waste water. The method of the invention comprises the following steps: (1) adding acid to the livestock and poultry farming waste water after biological treatment, and regulating the pH value of the waste water to be less than or equal to 5.0; (2) adding H2O2 and soluble ferrite to the waste water to carry out oxidation reaction, wherein the mol ratio of the H2O2 to the Fe<2+> in the soluble ferrite is between 1:1 to 2:1; and (3) after carrying out reaction for not less than 10 minutes, adding alkali to reaction liquid, regulating the pH of the reaction liquid to be greater than or equal to 6.0, carrying out flocculating and depositing, and then discharging deposit and waste water according with the discharge standard. By controlling the pH value of the reaction system, the adding proportion of the H2O2 to the FeSO4, and the running time, the invention achieves the treatment effect and optimization of energy consumption. The process effectively oxidizes and decomposes the trace organic pollutants (incretion interferents, antibiotics and the like), simultaneously removes various heavy metals and phosphorus by coagulating sedimentation, inactivates pathogenic bacteria in the waste water, and realizes integrated high-efficiency reinforcement treatment of the livestock and poultry farming waste water.

The invention provides a preparation method and an application method of a dummy template molecularly imprinted polymer selectively enriching bisphenol endocrine disruptors. The preparation method comprises the following steps: dissolving dummy template molecules, a crosslinking agent, a functional monomer and an initiator in a pore forming solvent to prepare a pre-polymerization solution, carrying out a polymerization reaction at 50-70DEG C for 24-48h; and grinding a white block polymer generated through the polymerization reaction, sieving, settling to obtain polymer particles with the particle size of 45-63[mu]m, carrying out soxhlet extraction or ultrasonic extraction with methanol/acetic acid and methanol as an extraction solvent, and removing residual template molecules and unreacted substances in order to obtain the dummy template molecularly imprinted polymer. The invention also provides a solid phase extraction filler. The filler is the dummy template molecularly imprinted polymer. The dummy template molecularly imprinted polymer has very strong specific selectivity and enrichment ability to bisphenol F, bisphenol E, bisphenol A, bisphenol B, bisphenol AF, tetrabromobisphenol A and other bisphenol compounds, solves the template bleeding problem during trace analysis, and realizes the rapid, senseitive, accurate and efficient detection of the bisphenol endocrine disruptors in environment, foods and biological samples.

The invention discloses a chitosan composite gel water purifying agent, which is gel prepared from solution of chitosan, cellulose, silica gel and activated carbon according to the mass ratio of 1:1-2.5:2-3.5:3-5. The invention also discloses a method for preparing the chitosan composite gel water purifying agent, which comprises the following steps of: adding the cellulose, the silica gel and the activated carbon into the solution of chitosan in turn; fully stirring at a high speed until black and homogeneous semifluid gel is formed, and obtaining the chitosan composite gel water purifying agent. The water purifying agent is natural and nontoxic, has functions of high-efficiency adsorption and flocculation, can be used for deep purification of an underwater environment polluted by endocrine disruptors and also can be used for emergency treatment in public health water pollution emergency. Meanwhile, the preparation method makes operation simple, reduces cost and is environmentally-friendly and suitable for industrial production.

The utility model relates to an ER Alpha activation-based PCR quantitative detection method on environment incretion interferent. The method comprises: the acceptor allosteric dimerization takes place after the combination of trace amount of environment incretion interferent and an estrogen acceptor; identify and combine a DNA dual-chain probe, which comprises kernel series 5-GGTCAnnnTGACC-3 of estrogen reaction elements; generate a ligand-ER-DNA probe compound, which is absorbed and separated with monoclonal antibody; and then carry out real-time fluorescent quantitative PCR quantity augmentation; finally evaluate the EDCs biological effect in the sample. The utility model method, with simple and convenient operation plus high sensitivity, can reach even exceed the HPLC-MS/GS analytical chemistry method, and can be extensively used in testing application for food, environment and other fields. The utility model method also relates to reagent kits; and has advantages of simple, convenient and sensitive operation in detection range of 100pM to 1nM.

Modified activated carbon for removing organic pollutants is prepared from the following raw materials: by weight, 80-90 parts of activated carbon, 6-8 parts of chitosan, 3-4 parts of Arabic gum, 1-2 parts of sodium alginate, 30-34 parts of sepiolite, 12-14 parts of zeolite, 8-10 parts of modified attapulgite and a proper amount of 20-25% hydrochloric acid and water. The modified activated carbon prepared by the method is used as an adsorbent, at the same time, the sepiolite, the zeolite and other substances with strong adsorption ability are added into the modified activated carbon, and the modified activated carbon can be used for removing the organic pollutants in a water treatment process, has the advantages of high purification capacity and long service life, and especially can solve the problem that water treatment methods in the prior art have poor operability on water polluted by endocrine disruptors of high concentration of phenols.

An object of the present invention is to provide a recording material or a recording sheet using, as a color-developing agent, a non-phenol compound that is a safe compound in no danger of corresponding to an endocrine disruptor and is good in color developing performance. The non-phenol compound used in the present invention is at least one selected from the group consisting of compounds represented by the following formulas (I) to (III).

The invention belongs to the technical field of feedwater treatment, and relates to a method for removing pollutants from drinking water. The method comprises the following steps of (1) adding potassium permanganate and a supported ruthenium catalyst simultaneously to unprocessed drinking water, and putting the above mixture in a contact reactor for reaction; and (2) separating the reacted catalyst and water with a settling tank to obtain the processed water. The added supported ruthenium catalyst can increase the efficiency of removing micro-pollutants by potassium permanganate. The oxidation rate for phenyl amine substances, phenol endocrine interferents and benzotriazole light stabilizing agents is increased by 200-800%; and the removal efficiency is increased by 15-200%, after reacting for 0.5-2.0 h in a nearly neutral condition.

The invention provides a preparation method and an application method of an ultra-high-selectivity bisphenol substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction sequentially by using methanol/acetic acid and methanol as extraction solvents; and residual substitute template molecules and un-reacted substances are removed, such that the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted solid-phase extraction material provided by the invention has high specific selectivity and enrichment capability upon bisphenol compounds. With the material, a template leakage problem during trace analysis is solved. The polymer can be used in separation and enrichment of bisphenol endocrine interferents in a complex substrate.

A Ru/Al2O3 catalyst for the catalytic oxidization of ozone is composed of Al2O3 as carrier and RuO2 as active component proportionally. Its preparing process includes such steps as equivolume mixing between RuCl3 solution and Al2O3 carrier, impregnating in shaker, drying and microwave heating. It has high effect to remove ozone, interference substance to internal secretion, phenols and micro-molecular acids.

The invention belongs to the technical field of a preparation and application of a primordial water hyacinth biomass carbon material with a porous structure, and specifically discloses a preparation method of a novel electrode material and an application of the electrode material as a cathode in an electro-Fenton system to degrade endocrine disruptors. The electrode material takes water hyacinth biomass powder as a precursor, zinc chloride powder is added in different proportions to perform activating, the novel biomass carbon material is prepared by high temperature calcination under the protection of an inert gas, and finally a porous electrode is obtained as the cathode material for E-Fenton to degrade endocrine disruptors. The material has relatively good electrical conductivity and relatively large specific surface area. The material can be used as a novel electrode material, and a good degradation effect is especially shown in the electro-Fenton experiment.

The invention belongs to the technical field of wastewater treatment, and discloses a preparation method of carbon and oxygen co-doped graphite phase carbon nitride, and an application of the carbon and oxygen co-doped graphite phase carbon nitride in the catalytic activation of persulfate to degrade pollutants in water. The carbon and oxygen co-doped graphite phase carbon nitride is prepared by high-temperature calcining of succinic acid and urea, is used as a catalyst to activate the persulfate to effectively degrade dyes, endocrine interferents, pesticides and other organic pollutants, hasa very good removal effect at a pH value of 3.0-9.0, and has an excellent stability, and a result of detection shows that active substances in the reaction system are hydroxy free radicals (<.>OH) andsulfate radicals (SO<4><.->) and singlet oxygen (<1>O2). The carbon and oxygen co-doped graphite phase carbon nitride has the advantages of no secondary pollution, convenience in separation from water and recycling, and broad application prospect when used for activating the persulfate to degrade the organic pollutants in water as a nonmetal catalyst.

The invention relates to a compound microbial preparation for degrading phenol endocrine disrupter and a preparation method, and relates to microorganism projects and sewage and waste water treatment processes. The compound microbial preparation comprises Klebsiella sp., azospirillum lipoferum, rhodococcuserythropolis and bacillus licheniformis. The microbial content of Klebsiella sp. accounts for 30%-50% of the total microbial content. The microbial content of azospirillum lipoferum accounts for 30%-50% of the total microbial content. The microbial content of rhodococcuserythropolis accounts for 10%-30% of the total microbial content. The microbial content of bacillus licheniformis accounts for 10%-30% of the total microbial content. The compound microbial preparation is fed into an anaerobic tank and an aerobic tank, and the sewage and waste water treatment processes are enhanced. The compound microbial flora can tolerate the high-concentration phenol endocrine disrupter which is used as organic matter needed in growth for being fully utilized and degraded, the sewage and waste water treatment processes are optimized, the secondary pollution of output water and sludge is reduced, and the safety of sludge landfill and agricultural utilization is improved.

The invention relates to a preparing method of an active carbon fiber loaded Ag/TiO2 compound catalytic material and application of the active carbon fiber loaded Ag/TiO2 compound catalytic material in strengthening an ozonation technology and guaranteeing water quality of regenerated water and micropollution surface water. The preparing method comprises the steps of preparing silver-titanium dioxide compound sol through a sol-gel technology, putting activated active carbon fiber into the sol to be soaked, and obtaining the active carbon fiber loaded Ag/TiO2 compound material through the procedures of heat treatment, water leaching, nitrogen sealing and illumination activating. The prepared material has a high micropore volume and a large external surface specific area, and the mineral catalysis and decomposition effects of conventional indexes, endocrine disrupter, polycyclic aromatic hydrocarbon and general organic matter in a water body are all superior to those of unloaded active carbon fiber catalysis materials.

Disclosed herein is a method for producing a transparent or opaque flooring sheet or a flooring using a styrene-butadiene copolymer resin (as a thermoplastic elastomer) by calendering. The flooring causes no problems associated with environmental hormones, and generates no halogen gases or endocrine disruptors, such as dioxin, during incineration. Further, the flooring can be regenerated and reused at any time, and exhibits superior stability upon installation and mounting and excellent elastic restoration after application of stress due to inherent flexibility of rubber.

The invention belongs to the technical field of preparation and application of an ordered mesoporous laminated-structure novel massive carbon material, and particularly discloses a preparation method of a novel activated-carbon-fiber-supported ordered mesoporous carbon-graphene composite material and application of the activated-carbon-fiber-supported ordered mesoporous carbon-graphene composite material as a cathode material in degrading endocrine disrupters by electric-Fenton reaction. The composite material is optimized on the basis of an ordered mesoporous-carbon-supported activated carbon fiber massive carbon material. The preparation method comprises the following steps: by using graphene oxide as a precursor of graphene, and adding into activated carbon fibers according to different proportions to obtain a graphene oxide-activated carbon fiber composite material; simultaneously dropwisely adding a phenolic resin as an precursor of ordered mesoporous carbon and F127 as a template onto GO@ACF according to a certain proportion; and carrying out solvent volatilization self-assembly, thermal polymerization and high-temperature calcination in an inert gas protective atmosphere to obtain the novel massive ordered-structure carbon material. The material has favorable electric conductivity and large specific area, and can be used as a novel electrode material.