30results about How to "High specific selectivity" patented technology

The invention provides a preparation method and an application method of a dummy template molecularly imprinted polymer selectively enriching bisphenol endocrine disruptors. The preparation method comprises the following steps: dissolving dummy template molecules, a crosslinking agent, a functional monomer and an initiator in a pore forming solvent to prepare a pre-polymerization solution, carrying out a polymerization reaction at 50-70DEG C for 24-48h; and grinding a white block polymer generated through the polymerization reaction, sieving, settling to obtain polymer particles with the particle size of 45-63[mu]m, carrying out soxhlet extraction or ultrasonic extraction with methanol/acetic acid and methanol as an extraction solvent, and removing residual template molecules and unreacted substances in order to obtain the dummy template molecularly imprinted polymer. The invention also provides a solid phase extraction filler. The filler is the dummy template molecularly imprinted polymer. The dummy template molecularly imprinted polymer has very strong specific selectivity and enrichment ability to bisphenol F, bisphenol E, bisphenol A, bisphenol B, bisphenol AF, tetrabromobisphenol A and other bisphenol compounds, solves the template bleeding problem during trace analysis, and realizes the rapid, senseitive, accurate and efficient detection of the bisphenol endocrine disruptors in environment, foods and biological samples.

The invention relates to a bisphenol A dummy template molecularly imprinted stir bar and a preparation method thereof. The stir bar is a glass bar containing an iron core, wherein the glass bar is coated by a polymer coating; the polymer coating is a bisphenol A dummy template molecularly imprinted polymer; modified silica microspheres with grain size being 400nm are taken as the carriers for synthesizing the imprinted polymer; and biphenyl diphenol is taken as a dummy template molecule. The bisphenol A dummy template molecularly imprinted polymer is prepared by carrying out surface grafting on the surfaces of silica microspheres with grain size being 400nm with vinyltrimethoxysilane to obtain the modified silica microspheres, then subjecting the modified silica microspheres to react withbiphenyl diphenol, 4-vinyl pyridine, ethylene glycol dimethacrylate and azodiisobutyronitrile and using a methanol-glacial acetic acid mixed solution to wash off the template molecule. The stir bar and the preparation method have the following beneficial effect that: SiO2 is taken as the carrier for synthesizing the imprinted polymer, thus greatly adding the number of the effective binding sites and improving the sensitivity.

The invention provides a preparation method and an application method of a fluoroquinolone antibiotic medicine substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction or ultrasonic extraction sequentially by using methanol/acetic acid and methanol as extraction solvents, such that residual substitute template molecules and interfering substances are removed, and the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted polymer has high specific selectivity and enrichment capability upon fluoroquinolone antibiotics. With the material, an imprinting effect is ensured, and a template leakage problem is solved. The polymer can be used in separation, enrichment and purification of fluoroquinolone antibiotics in complex substrate.

The invention provides a preparation method and an application method of an ultra-high-selectivity bisphenol substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction sequentially by using methanol/acetic acid and methanol as extraction solvents; and residual substitute template molecules and un-reacted substances are removed, such that the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted solid-phase extraction material provided by the invention has high specific selectivity and enrichment capability upon bisphenol compounds. With the material, a template leakage problem during trace analysis is solved. The polymer can be used in separation and enrichment of bisphenol endocrine interferents in a complex substrate.

The invention discloses a fluorescein diacetate fluorescence probe containing an aldehyde group structure and a preparation method and application thereof.The fluorescence probe is A-FDA, and the structural formula of A-FDA is shown in the formula (I).The A-FDA fluorescence probe is applied to indication for the activity of hydrolytic enzymes in living cells.By means of an aldehyde group in the probe, the lipophicity of the probe is improved, the specific selectivity of the probe is enhanced, reverse osmosis of hydrolyzed fluorescein generated after the probe enters the cells is relieved, and meanwhile good chemical stability, light stability, solubility and biocompatibility are achieved.A microimaging experiment shows that the probe has good cell targeting and permeability and has no toxic or side effect on the cells and organisms.The structural formula is shown in the description.

The invention provides an amphiphilic acetochlor magnetic molecularly imprinted polymer and a preparation method and application thereof. Glucose is used as a structural unit, thereby synthesizing hydrophilic alkenyl glycoside type functional monomer modified with imprinting acting site. By use of a hydrophilic-lyophobic dual-functional monomer molecular imprinting technology, the amphiphilic magnetic molecularly imprinted polymer is prepared. The prepared amphiphilic magnetic molecularly imprinted polymer has high specific selectivity, has amphipathicity and good adsorptive property in a water medium, and can be used for acetochlor content determination sample pretreatment in the agricultural products, and then an extraction system for complex sample pretreatment is established through the analytic detection and quantitative analysis of a liquid chromatogram system. The method provided by the invention is simple in step, convenient for operation, and strong in practicability.

The invention discloses an electrochemical sensor based on a carbon nanotube and a preparation method and application thereof and belongs to the field of environment analysis and electroanalytical chemistry. In a differential pulse voltammetry (DPV) mode, the electrochemical sensor can realize oxidation of interfering substances like catechol (CL, 0.188V) and hydroquinone (HQ, 0.092V) at low potential and does not cause interference to analysis of target substances (bisphenol A and bisphenol S) in the process of actual detection. In addition, peak potentials of the electrochemical sensor in the DPV mode to bisphenol A and bisphenol S are 0.472V and 0.736V, and difference is obvious, which is enough for realizing simultaneous detection of bisphenol A and bisphenol S. The electrochemical sensor has ultrahigh specific selectivity to bisphenol A and bisphenol S in complex matrixes, can effectively reduce matrix interference and can quickly, sensitively, accurately and efficiently analyze river water samples and thermosensitive paper samples.

The present invention provides an acrylate polymer capillary electrochromatography column decorated by macrocyclic polyamine and its preparing method, the column of acrylate polymer organic polymer decorated by macrocyclic polyamine is prepared by free radical polymerization and method of column back decoration, the whole surface of the acrylate polymer in the electrochromatography column is linked with a macrocyclic polyamine, the macrocyclic polyamine is 1, 4, 10, 13, 16-quinquevalent nitrogen trinuclear heneicosane-9, 17-diketone. The preparing method of acrylate polymer capillary electrochromatography column decorated by macrocyclic polyamine is simple, having better reproduction quality, the capillary electrochromatography column has a better separating effect to inorganic anions, benzenediol isomer, benzoic acid derivatives and ribonucleotide, having better application in the separating aspect for complicated component material.

The invention discloses a polypeptide capable of specifically targeting triple-negative breast cancer stem cells and application thereof. The polypeptide has an amino acid sequence shown in any one ofSEQ ID No.1 to SEQ ID No. 3. The polypeptide provided by an embodiment of the invention has high specificity and affinity to the triple-negative breast cancer stem cells, has weak binding capacity toother types of breast cancer cells, shows high specificity and selectivity to the triple-negative breast cancer stem cells, and can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a fluorescent functional monomer, a molecularly imprinted polymer, and a preparation method and an application thereof, which belong to the technical field of a high polymer material. The fluorescent functional monomer 9,10-di(1-vinyl-3-imidazolium chloride) methylanthracene is dissolved in anhydrous acetonitrile by 9,10-dichloromethyl anthracene and 1-vinyl imidazole, thematerial is heated, and is subjected to backflow. Compared with 9,10-dichloromethyl anthracene, the fluorescence intensity the fluorescent functional monomer is significantly enhanced, can be used asa fluorescent monomer, and contains a group capable of recognizing template molecule 2,4,6-trichlorophenol. Further, the modified ionic liquid is used as the fluorescent functional monomer, and polymerized with the template molecule and a crosslinking agent under the action of an initiator, and forms the molecularly imprinted polymer. The molecularly imprinted polymer realizes double recognition of the target molecule, has high selectivity and disturbing ability, and has great application value in detecting 2,4,6-trichlorophenol in the environmental water body.

The invention discloses a polypeptide capable of specifically targeting triple-negative breast cancer cells, and an application thereof. The polypeptide has an amino acid sequence represented by any one of SEQ ID No.1 to SEQ ID No.5. The polypeptide provided by the embodiment of the invention has high specificity and affinity to the triple-negative breast cancer cells, has weak binding capacity toother types of breast cancer cells, shows high-specificity selection to triple-negative breast cancer cells, and can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a polypeptide specifically targeting triple-negative breast cancer and its application. The polypeptide has the amino acid sequence shown in SEQ ID No.1. The polypeptide has homology with Decorin, and can bind to Decorin receptors on the surface of triple-negative breast cancer cancer cells to play a targeting role. At the same time, the polypeptide has a weak binding ability to other types of breast cancer cells, showing Higher specificity and selectivity can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a preparation method of an electrochemical sensor for detecting sepsis marker procalcitonin. The preparation method comprises the following steps: preparing carbon nitride powder, preparing graphite-phase carbon nitride, modifying an electrode with the graphite-phase carbon nitride, and modifying the electrode with a polypeptide probe. According to the electrochemical sensor prepared in the invention, the exfoliated graphite phase carbon nitride is modified on the surface of the glassy carbon electrode, so that the specific surface area and the electron transfer capability can be increased during detection, and the polypeptide probe capable of specifically identifying procalcitonin is modified on the glassy carbon electrode, so the detection sensitivity, accuracy and characteristic selectivity of the sensor can be improved conveniently, and the detection sensitivity of the procalcitonin sensor to procalcitonin can reach 0.15 fg/mL.