30results about How to "High specific selectivity" patented technology

The invention provides a preparation method and an application method of a dummy template molecularly imprinted polymer selectively enriching bisphenol endocrine disruptors. The preparation method comprises the following steps: dissolving dummy template molecules, a crosslinking agent, a functional monomer and an initiator in a pore forming solvent to prepare a pre-polymerization solution, carrying out a polymerization reaction at 50-70DEG C for 24-48h; and grinding a white block polymer generated through the polymerization reaction, sieving, settling to obtain polymer particles with the particle size of 45-63[mu]m, carrying out soxhlet extraction or ultrasonic extraction with methanol / acetic acid and methanol as an extraction solvent, and removing residual template molecules and unreacted substances in order to obtain the dummy template molecularly imprinted polymer. The invention also provides a solid phase extraction filler. The filler is the dummy template molecularly imprinted polymer. The dummy template molecularly imprinted polymer has very strong specific selectivity and enrichment ability to bisphenol F, bisphenol E, bisphenol A, bisphenol B, bisphenol AF, tetrabromobisphenol A and other bisphenol compounds, solves the template bleeding problem during trace analysis, and realizes the rapid, senseitive, accurate and efficient detection of the bisphenol endocrine disruptors in environment, foods and biological samples.

The invention relates to a bisphenol A dummy template molecularly imprinted stir bar and a preparation method thereof. The stir bar is a glass bar containing an iron core, wherein the glass bar is coated by a polymer coating; the polymer coating is a bisphenol A dummy template molecularly imprinted polymer; modified silica microspheres with grain size being 400nm are taken as the carriers for synthesizing the imprinted polymer; and biphenyl diphenol is taken as a dummy template molecule. The bisphenol A dummy template molecularly imprinted polymer is prepared by carrying out surface grafting on the surfaces of silica microspheres with grain size being 400nm with vinyltrimethoxysilane to obtain the modified silica microspheres, then subjecting the modified silica microspheres to react withbiphenyl diphenol, 4-vinyl pyridine, ethylene glycol dimethacrylate and azodiisobutyronitrile and using a methanol-glacial acetic acid mixed solution to wash off the template molecule. The stir bar and the preparation method have the following beneficial effect that: SiO2 is taken as the carrier for synthesizing the imprinted polymer, thus greatly adding the number of the effective binding sites and improving the sensitivity.

The invention provides a preparation method and an application method of a fluoroquinolone antibiotic medicine substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction or ultrasonic extraction sequentially by using methanol / acetic acid and methanol as extraction solvents, such that residual substitute template molecules and interfering substances are removed, and the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted polymer has high specific selectivity and enrichment capability upon fluoroquinolone antibiotics. With the material, an imprinting effect is ensured, and a template leakage problem is solved. The polymer can be used in separation, enrichment and purification of fluoroquinolone antibiotics in complex substrate.

The invention provides a preparation method and an application method of an ultra-high-selectivity bisphenol substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction sequentially by using methanol / acetic acid and methanol as extraction solvents; and residual substitute template molecules and un-reacted substances are removed, such that the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted solid-phase extraction material provided by the invention has high specific selectivity and enrichment capability upon bisphenol compounds. With the material, a template leakage problem during trace analysis is solved. The polymer can be used in separation and enrichment of bisphenol endocrine interferents in a complex substrate.

The invention discloses a preparation method of carbofuran molecularly imprinted microspheres, comprising the following steps of: dissolving template molecules and a functional monomer into a pore-forming agent solution containing a cross-linking agent and an initiator so as to obtain a reaction mixture, carrying out ultrasonic degassing, sparging nitrogen for deoxygenation, carrying out polymerization in a sealed state, carrying out centrifugation after complete reaction so as to obtain a white powdery polymer, carrying out Soxhlet extraction respectively by the adoption of a methanol acetate mixed liquor and methanol as extraction solvents, drying under vacuum, and removing the solvents so as to obtain the carbofuran molecularly imprinted microspheres. The invention also discloses the carbofuran molecularly imprinted microspheres prepared by the preparation method and an application thereof. The carbofuran molecularly imprinted microspheres obtained in the invention has specific selectivity for carbofuran in agricultural products, can be used to detect residual pesticides on agricultural products rapidly, sensitively, accurately and efficiently, and also can be used as a solid-phase extraction filler.

The invention discloses a climbazole alternative template molecularly imprinted polymer as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving an alternative template molecule, a cross-linking agent, a functional monomer and an initiator into a pore-forming solvent to obtain a prepolymerized solution, and carrying out polymerization reaction at the temperature of 55 to 75DEG C for 24 to 48 hours; grinding, sieving and precipitating a white block polymer generated by the polymerization reaction to obtain polymer white particleswith the particle size of 30 to 60mu m; carrying out Soxhlet extraction on obtained polymer particles by sequentially adopting methanol / acetic acid and methanol as extracting solvents and removing thealternative template molecule and unreacted substances to obtain the alternative template molecularly imprinted polymer. The alternative template molecularly imprinted polymer provided by the invention can be used as a solid phase extraction material, is applied to enrichment and purification of climbazole in environmental samples, food and personal caring products, has the advantages of high selectivity, high adsorption capacity and no template leakage, and has important significance for human health and environmental monitoring.

The invention discloses a fluorescein diacetate fluorescence probe containing an aldehyde group structure and a preparation method and application thereof.The fluorescence probe is A-FDA, and the structural formula of A-FDA is shown in the formula (I).The A-FDA fluorescence probe is applied to indication for the activity of hydrolytic enzymes in living cells.By means of an aldehyde group in the probe, the lipophicity of the probe is improved, the specific selectivity of the probe is enhanced, reverse osmosis of hydrolyzed fluorescein generated after the probe enters the cells is relieved, and meanwhile good chemical stability, light stability, solubility and biocompatibility are achieved.A microimaging experiment shows that the probe has good cell targeting and permeability and has no toxic or side effect on the cells and organisms.The structural formula is shown in the description.

The invention provides an amphiphilic acetochlor magnetic molecularly imprinted polymer and a preparation method and application thereof. Glucose is used as a structural unit, thereby synthesizing hydrophilic alkenyl glycoside type functional monomer modified with imprinting acting site. By use of a hydrophilic-lyophobic dual-functional monomer molecular imprinting technology, the amphiphilic magnetic molecularly imprinted polymer is prepared. The prepared amphiphilic magnetic molecularly imprinted polymer has high specific selectivity, has amphipathicity and good adsorptive property in a water medium, and can be used for acetochlor content determination sample pretreatment in the agricultural products, and then an extraction system for complex sample pretreatment is established through the analytic detection and quantitative analysis of a liquid chromatogram system. The method provided by the invention is simple in step, convenient for operation, and strong in practicability.

The invention discloses an electrochemical sensor based on a carbon nanotube and a preparation method and application thereof and belongs to the field of environment analysis and electroanalytical chemistry. In a differential pulse voltammetry (DPV) mode, the electrochemical sensor can realize oxidation of interfering substances like catechol (CL, 0.188V) and hydroquinone (HQ, 0.092V) at low potential and does not cause interference to analysis of target substances (bisphenol A and bisphenol S) in the process of actual detection. In addition, peak potentials of the electrochemical sensor in the DPV mode to bisphenol A and bisphenol S are 0.472V and 0.736V, and difference is obvious, which is enough for realizing simultaneous detection of bisphenol A and bisphenol S. The electrochemical sensor has ultrahigh specific selectivity to bisphenol A and bisphenol S in complex matrixes, can effectively reduce matrix interference and can quickly, sensitively, accurately and efficiently analyze river water samples and thermosensitive paper samples.

The invention discloses a fluorescent functional monomer, a molecularly imprinted polymer, and a preparation method and an application thereof, which belong to the technical field of a high polymer material. The fluorescent functional monomer 9,10-di(1-vinyl-3-imidazolium chloride) methylanthracene is dissolved in anhydrous acetonitrile by 9,10-dichloromethyl anthracene and 1-vinyl imidazole, thematerial is heated, and is subjected to backflow. Compared with 9,10-dichloromethyl anthracene, the fluorescence intensity the fluorescent functional monomer is significantly enhanced, can be used asa fluorescent monomer, and contains a group capable of recognizing template molecule 2,4,6-trichlorophenol. Further, the modified ionic liquid is used as the fluorescent functional monomer, and polymerized with the template molecule and a crosslinking agent under the action of an initiator, and forms the molecularly imprinted polymer. The molecularly imprinted polymer realizes double recognition of the target molecule, has high selectivity and disturbing ability, and has great application value in detecting 2,4,6-trichlorophenol in the environmental water body.

The invention relates to the technical field of sensors, and provides a gas sensor and a manufacturing method thereof. The gas sensor comprises a substrate, a graphene layer, a metal electrode pair and a metal oxide layer, the substrate, the graphene layer and the metal electrode pair are sequentially arranged from bottom to top; one metal electrode pair comprises two metal electrodes, and an area between the two metal electrodes of one metal electrode pair is a channel; a gap groove is formed in the graphene layer at the channel, so that the graphene layer at the channel is disconnected, and a graphene electrode pair is formed; the metal oxide layer is located at the channel and covers the graphene electrode pair and the gap groove so as to be connected with the graphene electrode pair; the area of the metal oxide layer is smaller than that of the channel, so that the channel is internally provided with the exposed graphene layer which is not covered by the metal oxide layer. The invention provides the gas sensor which is high in response strength and good in response specific selectivity.

The present invention provides an acrylate polymer capillary electrochromatography column decorated by macrocyclic polyamine and its preparing method, the column of acrylate polymer organic polymer decorated by macrocyclic polyamine is prepared by free radical polymerization and method of column back decoration, the whole surface of the acrylate polymer in the electrochromatography column is linked with a macrocyclic polyamine, the macrocyclic polyamine is 1, 4, 10, 13, 16-quinquevalent nitrogen trinuclear heneicosane-9, 17-diketone. The preparing method of acrylate polymer capillary electrochromatography column decorated by macrocyclic polyamine is simple, having better reproduction quality, the capillary electrochromatography column has a better separating effect to inorganic anions, benzenediol isomer, benzoic acid derivatives and ribonucleotide, having better application in the separating aspect for complicated component material.

The invention discloses a polypeptide capable of specifically targeting triple-negative breast cancer stem cells and application thereof. The polypeptide has an amino acid sequence shown in any one ofSEQ ID No.1 to SEQ ID No. 3. The polypeptide provided by an embodiment of the invention has high specificity and affinity to the triple-negative breast cancer stem cells, has weak binding capacity toother types of breast cancer cells, shows high specificity and selectivity to the triple-negative breast cancer stem cells, and can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a fluorescent functional monomer, a molecularly imprinted polymer, and a preparation method and an application thereof, which belong to the technical field of a high polymer material. The fluorescent functional monomer 9,10-di(1-vinyl-3-imidazolium chloride) methylanthracene is dissolved in anhydrous acetonitrile by 9,10-dichloromethyl anthracene and 1-vinyl imidazole, thematerial is heated, and is subjected to backflow. Compared with 9,10-dichloromethyl anthracene, the fluorescence intensity the fluorescent functional monomer is significantly enhanced, can be used asa fluorescent monomer, and contains a group capable of recognizing template molecule 2,4,6-trichlorophenol. Further, the modified ionic liquid is used as the fluorescent functional monomer, and polymerized with the template molecule and a crosslinking agent under the action of an initiator, and forms the molecularly imprinted polymer. The molecularly imprinted polymer realizes double recognition of the target molecule, has high selectivity and disturbing ability, and has great application value in detecting 2,4,6-trichlorophenol in the environmental water body.

The invention relates to a bisphenol A dummy template molecularly imprinted stir bar and a preparation method thereof. The stir bar is a glass bar containing an iron core, wherein the glass bar is coated by a polymer coating; the polymer coating is a bisphenol A dummy template molecularly imprinted polymer; modified silica microspheres with grain size being 400nm are taken as the carriers for synthesizing the imprinted polymer; and biphenyl diphenol is taken as a dummy template molecule. The bisphenol A dummy template molecularly imprinted polymer is prepared by carrying out surface grafting on the surfaces of silica microspheres with grain size being 400nm with vinyltrimethoxysilane to obtain the modified silica microspheres, then subjecting the modified silica microspheres to react withbiphenyl diphenol, 4-vinyl pyridine, ethylene glycol dimethacrylate and azodiisobutyronitrile and using a methanol-glacial acetic acid mixed solution to wash off the template molecule. The stir bar and the preparation method have the following beneficial effect that: SiO2 is taken as the carrier for synthesizing the imprinted polymer, thus greatly adding the number of the effective binding sites and improving the sensitivity.

A process for creating a detection system of HBV DNA includes such steps as designing oligonucleotide probe by using the 1873-1912 sites of HBV DNA as target sequence, dividing the probe into two parts (one including 20 nucleotides and another including 6 nucleotides), using the paraffin thiol to decorate the 5' or 3' terminal of oligonucleotide, using Au-S bone to link to probe to Au or Fe3O4 / Au nanoparticle, using manipulated capture probe, and adding linking chain and signal amplifying substances in the detection system. Its advantages are high sensitivity and specificity and low cost.

The invention discloses a polypeptide capable of specifically targeting triple-negative breast cancer cells, and an application thereof. The polypeptide has an amino acid sequence represented by any one of SEQ ID No.1 to SEQ ID No.5. The polypeptide provided by the embodiment of the invention has high specificity and affinity to the triple-negative breast cancer cells, has weak binding capacity toother types of breast cancer cells, shows high-specificity selection to triple-negative breast cancer cells, and can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a climbazole alternative template molecularly imprinted polymer as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving an alternative template molecule, a cross-linking agent, a functional monomer and an initiator into a pore-forming solvent to obtain a prepolymerized solution, and carrying out polymerization reaction at the temperature of 55 to 75DEG C for 24 to 48 hours; grinding, sieving and precipitating a white block polymer generated by the polymerization reaction to obtain polymer white particleswith the particle size of 30 to 60mu m; carrying out Soxhlet extraction on obtained polymer particles by sequentially adopting methanol / acetic acid and methanol as extracting solvents and removing thealternative template molecule and unreacted substances to obtain the alternative template molecularly imprinted polymer. The alternative template molecularly imprinted polymer provided by the invention can be used as a solid phase extraction material, is applied to enrichment and purification of climbazole in environmental samples, food and personal caring products, has the advantages of high selectivity, high adsorption capacity and no template leakage, and has important significance for human health and environmental monitoring.

The invention discloses a polypeptide specifically targeting triple-negative breast cancer and its application. The polypeptide has the amino acid sequence shown in SEQ ID No.1. The polypeptide has homology with Decorin, and can bind to Decorin receptors on the surface of triple-negative breast cancer cancer cells to play a targeting role. At the same time, the polypeptide has a weak binding ability to other types of breast cancer cells, showing Higher specificity and selectivity can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a polypeptide capable of specifically targeting triple negative breast cancer cells and the application of the polypeptide. The polypeptide has the amino acid sequence shown in any one of SEQ ID No.1 to SEQ ID No.5. The polypeptide provided by the embodiment of the present invention has high specificity and affinity to triple-negative breast cancer cells, but has relatively weak binding ability to other types of breast cancer cells, showing high specificity for triple-negative breast cancer cells, It can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a pyrethroid insecticide molecular imprinting polymer as well as a preparation method and an application thereof. Pyrethroid is taken as a template molecule, 1,3,5-tris(4-aminophenyl)benzene and 2,4,6-triformylphloroglucinol are taken as functional monomers, amidogen and a formyl group are subjected to a condensation reaction in a pore-foaming agent, a polymer with the template molecule is obtained, the template molecule is eluted, and the pyrethroid insecticide molecular imprinting polymer can be obtained. The molecular imprinting polymer adopting the stereo structure is obtained for the first time through a chemical reaction, damage of free radical polymerization to alkence double bonds is prevented, and carbon and nitrogen double bonds are formed, so that the bonding strength of a target molecule and the polymer is increased. The prepared molecular imprinting polymer has high specific selectivity and good adsorption performance.

The invention discloses a polypeptide capable of specifically targeting triple-negative breast cancer stem cells and the application of the polypeptide. The polypeptide has the amino acid sequence shown in any one of SEQ ID No.1 to SEQ ID No.3. The polypeptide provided by the embodiment of the present invention has high specificity and affinity with triple-negative breast cancer stem cells, but has relatively weak binding ability with other types of breast cancer cells, showing high specificity for triple-negative breast cancer stem cells, It can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention provides a preparation method and an application method of an ultra-high-selectivity bisphenol substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction sequentially by using methanol / acetic acid and methanol as extraction solvents; and residual substitute template molecules and un-reacted substances are removed, such that the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted solid-phase extraction material provided by the invention has high specific selectivity and enrichment capability upon bisphenol compounds. With the material, a template leakage problem during trace analysis is solved. The polymer can be used in separation and enrichment of bisphenol endocrine interferents in a complex substrate.

The invention discloses a preparation method and application of a molecularly imprinted polymer-ferroferric oxide-based electrochemiluminescence sensor. The preparation method is characterized by comprising the following steps of: preparing Fe3O4 nanocrystals; sequentially adding cyclohexane, TritonX-100, a Fe3O4-NCs solution, TEOS and ammonia water into in a fixed two-necked flask, reacting by stirring for 2h, then adding a BPA (Bis Phenol-A) template molecule acetonitrile solution and 3-aminopropyltriethoxysilane, reacting for 12h, and washing so as to facilitate the falling of template molecules, thereby obtaining precipitate, namely Fe3O4-NCs-MIPs; and sequentially dropping a Fe3O4-NCs-MIPs solution and a perfluorosulfonic acid ethanol solution onto the surface of a glassy carbon electrode to obtain a working electrode Nafion / Fe3O4-NCs-MIPs / GCE. The preparation method has the advantages of good selectivity, stability and high sensitivity.

The invention provides an amphiphilic acetochlor magnetic molecularly imprinted polymer and a preparation method and application thereof. Glucose is used as a structural unit, thereby synthesizing hydrophilic alkenyl glycoside type functional monomer modified with imprinting acting site. By use of a hydrophilic-lyophobic dual-functional monomer molecular imprinting technology, the amphiphilic magnetic molecularly imprinted polymer is prepared. The prepared amphiphilic magnetic molecularly imprinted polymer has high specific selectivity, has amphipathicity and good adsorptive property in a water medium, and can be used for acetochlor content determination sample pretreatment in the agricultural products, and then an extraction system for complex sample pretreatment is established through the analytic detection and quantitative analysis of a liquid chromatogram system. The method provided by the invention is simple in step, convenient for operation, and strong in practicability.

The invention discloses a preparation method of carbofuran molecularly imprinted microspheres, comprising the following steps of: dissolving template molecules and a functional monomer into a pore-forming agent solution containing a cross-linking agent and an initiator so as to obtain a reaction mixture, carrying out ultrasonic degassing, sparging nitrogen for deoxygenation, carrying out polymerization in a sealed state, carrying out centrifugation after complete reaction so as to obtain a white powdery polymer, carrying out Soxhlet extraction respectively by the adoption of a methanol acetate mixed liquor and methanol as extraction solvents, drying under vacuum, and removing the solvents so as to obtain the carbofuran molecularly imprinted microspheres. The invention also discloses the carbofuran molecularly imprinted microspheres prepared by the preparation method and an application thereof. The carbofuran molecularly imprinted microspheres obtained in the invention has specific selectivity for carbofuran in agricultural products, can be used to detect residual pesticides on agricultural products rapidly, sensitively, accurately and efficiently, and also can be used as a solid-phase extraction filler.

The present invention provides a method for preparing a high-selectivity bisphenol-based substituted template molecular imprinting polymer by adding a sterically-hindered monomer, and uses of the polymer. The preparation method comprises: dissolving a substituted template molecule, a cross-linking agent, a functional monomer, a sterically-hindered monomer and an initiator in a pore forming agent to form a uniform pre-polymerization solution, placing the solution for a certain time at a low temperature, carrying out a polymerization reaction for 20-48 h at a temperature of 50-70 DEG C, grindingthe white block-like polymer produced by the polymerization reaction, screening, settling to obtain polymer particles with a particle size of 38-63 [mu]m, carrying out Soxhlet extraction on the obtained particles by using a methanol / acetic acid mixing solution or methanol as an extractant, and removing the substituted template and the unreacted material from the polymer so as to obtain the molecular imprinting polymer. According to the present invention, by adding the sterically-hindered monomer, the specific selectivity and the enrichment ability of the polymer to the bisphenol-based compound are enhanced while the anti-interference ability of the material to the interferent is improved.

The invention discloses a preparation method of an electrochemical sensor for detecting sepsis marker procalcitonin. The preparation method comprises the following steps: preparing carbon nitride powder, preparing graphite-phase carbon nitride, modifying an electrode with the graphite-phase carbon nitride, and modifying the electrode with a polypeptide probe. According to the electrochemical sensor prepared in the invention, the exfoliated graphite phase carbon nitride is modified on the surface of the glassy carbon electrode, so that the specific surface area and the electron transfer capability can be increased during detection, and the polypeptide probe capable of specifically identifying procalcitonin is modified on the glassy carbon electrode, so the detection sensitivity, accuracy and characteristic selectivity of the sensor can be improved conveniently, and the detection sensitivity of the procalcitonin sensor to procalcitonin can reach 0.15 fg / mL.

The invention belongs to the technical field of separation and analysis, and relates to a perfluorooctane sulfonic acid molecularly imprinted polymer as well as a preparation method and application thereof, and the preparation method comprises the following steps: uniformly mixing perfluorooctane sulfonic acid, 1, 4-benzene diacetonitrile, 1, 4-di (4-formyl phenyl) benzene and a pore-foaming agent to obtain a mixed solution; degassing the mixed solution, and carrying out condensation reaction in the presence of a catalyst to obtain a polymer with template molecules; and removing the template molecules from the polymer with the template molecules, and drying to obtain the polymer with the template molecules. The molecularly imprinted polymer with high specific selectivity and good adsorption performance can be prepared, and the molecularly imprinted polymer can be used for pretreatment of a sample for determining the content of perfluorooctane sulfonic acid in food, and then analysis detection and quantitative analysis are performed through a liquid chromatography-mass spectrometry system.