30results about How to "High specific selectivity" patented technology

The invention discloses a fluorescent functional monomer, a molecularly imprinted polymer, and a preparation method and an application thereof, which belong to the technical field of a high polymer material. The fluorescent functional monomer 9,10-di(1-vinyl-3-imidazolium chloride) methylanthracene is dissolved in anhydrous acetonitrile by 9,10-dichloromethyl anthracene and 1-vinyl imidazole, thematerial is heated, and is subjected to backflow. Compared with 9,10-dichloromethyl anthracene, the fluorescence intensity the fluorescent functional monomer is significantly enhanced, can be used asa fluorescent monomer, and contains a group capable of recognizing template molecule 2,4,6-trichlorophenol. Further, the modified ionic liquid is used as the fluorescent functional monomer, and polymerized with the template molecule and a crosslinking agent under the action of an initiator, and forms the molecularly imprinted polymer. The molecularly imprinted polymer realizes double recognition of the target molecule, has high selectivity and disturbing ability, and has great application value in detecting 2,4,6-trichlorophenol in the environmental water body.

The invention relates to a bisphenol A dummy template molecularly imprinted stir bar and a preparation method thereof. The stir bar is a glass bar containing an iron core, wherein the glass bar is coated by a polymer coating; the polymer coating is a bisphenol A dummy template molecularly imprinted polymer; modified silica microspheres with grain size being 400nm are taken as the carriers for synthesizing the imprinted polymer; and biphenyl diphenol is taken as a dummy template molecule. The bisphenol A dummy template molecularly imprinted polymer is prepared by carrying out surface grafting on the surfaces of silica microspheres with grain size being 400nm with vinyltrimethoxysilane to obtain the modified silica microspheres, then subjecting the modified silica microspheres to react withbiphenyl diphenol, 4-vinyl pyridine, ethylene glycol dimethacrylate and azodiisobutyronitrile and using a methanol-glacial acetic acid mixed solution to wash off the template molecule. The stir bar and the preparation method have the following beneficial effect that: SiO2 is taken as the carrier for synthesizing the imprinted polymer, thus greatly adding the number of the effective binding sites and improving the sensitivity.

A process for creating a detection system of HBV DNA includes such steps as designing oligonucleotide probe by using the 1873-1912 sites of HBV DNA as target sequence, dividing the probe into two parts (one including 20 nucleotides and another including 6 nucleotides), using the paraffin thiol to decorate the 5' or 3' terminal of oligonucleotide, using Au-S bone to link to probe to Au or Fe3O4 / Au nanoparticle, using manipulated capture probe, and adding linking chain and signal amplifying substances in the detection system. Its advantages are high sensitivity and specificity and low cost.

The invention discloses a climbazole alternative template molecularly imprinted polymer as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving an alternative template molecule, a cross-linking agent, a functional monomer and an initiator into a pore-forming solvent to obtain a prepolymerized solution, and carrying out polymerization reaction at the temperature of 55 to 75DEG C for 24 to 48 hours; grinding, sieving and precipitating a white block polymer generated by the polymerization reaction to obtain polymer white particleswith the particle size of 30 to 60mu m; carrying out Soxhlet extraction on obtained polymer particles by sequentially adopting methanol / acetic acid and methanol as extracting solvents and removing thealternative template molecule and unreacted substances to obtain the alternative template molecularly imprinted polymer. The alternative template molecularly imprinted polymer provided by the invention can be used as a solid phase extraction material, is applied to enrichment and purification of climbazole in environmental samples, food and personal caring products, has the advantages of high selectivity, high adsorption capacity and no template leakage, and has important significance for human health and environmental monitoring.

The invention discloses a polypeptide capable of specifically targeting triple negative breast cancer cells and the application of the polypeptide. The polypeptide has the amino acid sequence shown in any one of SEQ ID No.1 to SEQ ID No.5. The polypeptide provided by the embodiment of the present invention has high specificity and affinity to triple-negative breast cancer cells, but has relatively weak binding ability to other types of breast cancer cells, showing high specificity for triple-negative breast cancer cells, It can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention discloses a pyrethroid insecticide molecular imprinting polymer as well as a preparation method and an application thereof. Pyrethroid is taken as a template molecule, 1,3,5-tris(4-aminophenyl)benzene and 2,4,6-triformylphloroglucinol are taken as functional monomers, amidogen and a formyl group are subjected to a condensation reaction in a pore-foaming agent, a polymer with the template molecule is obtained, the template molecule is eluted, and the pyrethroid insecticide molecular imprinting polymer can be obtained. The molecular imprinting polymer adopting the stereo structure is obtained for the first time through a chemical reaction, damage of free radical polymerization to alkence double bonds is prevented, and carbon and nitrogen double bonds are formed, so that the bonding strength of a target molecule and the polymer is increased. The prepared molecular imprinting polymer has high specific selectivity and good adsorption performance.

The invention discloses a polypeptide capable of specifically targeting triple-negative breast cancer stem cells and the application of the polypeptide. The polypeptide has the amino acid sequence shown in any one of SEQ ID No.1 to SEQ ID No.3. The polypeptide provided by the embodiment of the present invention has high specificity and affinity with triple-negative breast cancer stem cells, but has relatively weak binding ability with other types of breast cancer cells, showing high specificity for triple-negative breast cancer stem cells, It can be used for targeted therapy or imaging diagnosis of triple-negative breast cancer.

The invention provides a preparation method and an application method of an ultra-high-selectivity bisphenol substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction sequentially by using methanol / acetic acid and methanol as extraction solvents; and residual substitute template molecules and un-reacted substances are removed, such that the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted solid-phase extraction material provided by the invention has high specific selectivity and enrichment capability upon bisphenol compounds. With the material, a template leakage problem during trace analysis is solved. The polymer can be used in separation and enrichment of bisphenol endocrine interferents in a complex substrate.

The invention discloses a preparation method and application of a molecularly imprinted polymer-ferroferric oxide-based electrochemiluminescence sensor. The preparation method is characterized by comprising the following steps of: preparing Fe3O4 nanocrystals; sequentially adding cyclohexane, TritonX-100, a Fe3O4-NCs solution, TEOS and ammonia water into in a fixed two-necked flask, reacting by stirring for 2h, then adding a BPA (Bis Phenol-A) template molecule acetonitrile solution and 3-aminopropyltriethoxysilane, reacting for 12h, and washing so as to facilitate the falling of template molecules, thereby obtaining precipitate, namely Fe3O4-NCs-MIPs; and sequentially dropping a Fe3O4-NCs-MIPs solution and a perfluorosulfonic acid ethanol solution onto the surface of a glassy carbon electrode to obtain a working electrode Nafion / Fe3O4-NCs-MIPs / GCE. The preparation method has the advantages of good selectivity, stability and high sensitivity.

The invention discloses a surface-enhanced Raman spectrum detection method for melamine and formaldehyde. The formaldehyde SERS substrate of the invention has specific selectivity, high sensitivity, signal stability and reproducibility. The preparation method of the formaldehyde SERS substrate is simple, the cost is low, and the detection kit made into it is convenient to carry and store. In the SERS detection method of melamine and formaldehyde, two substrates are used, and the detection of melamine and formaldehyde can be realized by preparing the test solution once.

The invention provides an amphiphilic acetochlor magnetic molecularly imprinted polymer and a preparation method and application thereof. Glucose is used as a structural unit, thereby synthesizing hydrophilic alkenyl glycoside type functional monomer modified with imprinting acting site. By use of a hydrophilic-lyophobic dual-functional monomer molecular imprinting technology, the amphiphilic magnetic molecularly imprinted polymer is prepared. The prepared amphiphilic magnetic molecularly imprinted polymer has high specific selectivity, has amphipathicity and good adsorptive property in a water medium, and can be used for acetochlor content determination sample pretreatment in the agricultural products, and then an extraction system for complex sample pretreatment is established through the analytic detection and quantitative analysis of a liquid chromatogram system. The method provided by the invention is simple in step, convenient for operation, and strong in practicability.

The invention discloses a preparation method of carbofuran molecularly imprinted microspheres, comprising the following steps of: dissolving template molecules and a functional monomer into a pore-forming agent solution containing a cross-linking agent and an initiator so as to obtain a reaction mixture, carrying out ultrasonic degassing, sparging nitrogen for deoxygenation, carrying out polymerization in a sealed state, carrying out centrifugation after complete reaction so as to obtain a white powdery polymer, carrying out Soxhlet extraction respectively by the adoption of a methanol acetate mixed liquor and methanol as extraction solvents, drying under vacuum, and removing the solvents so as to obtain the carbofuran molecularly imprinted microspheres. The invention also discloses the carbofuran molecularly imprinted microspheres prepared by the preparation method and an application thereof. The carbofuran molecularly imprinted microspheres obtained in the invention has specific selectivity for carbofuran in agricultural products, can be used to detect residual pesticides on agricultural products rapidly, sensitively, accurately and efficiently, and also can be used as a solid-phase extraction filler.