12 results about "Capillary electrochromatography" patented technology

The invention discloses a capillary electrochromatography monolithic column based on gold nanoflower modification and application of the capillary electrochromatography monolithic column in chiral separation, and belongs to the field of pharmaceutical analysis. The method comprises the following steps: synthesizing guanylic acid-coated chiral gold nanoflowers in a chiral environment constructed by guanylic acid chiral molecules and an L-ascorbic acid chiral reducing agent, immobilizing the guanylic acid-coated chiral gold nanoflowers on an amino-functionalized capillary silica gel monolithic column as a chiral selective agent, and separating a chiral drug under an electrophoresis condition. And baseline separation of propranolol enantiomers can be realized. The separation method is simple to operate, and a novel high-selectivity chiral separation detection technology is established.

The present invention relates to a method for separating galactose end group diastereomers by capillary electrochromatography, and belongs to the technical field of chromatographic analysis. The present invention forms a negatively charged complex with galactose by using borax electrophoresis buffer, which can improve the absorbance of galactose at ultraviolet 195nm, and realize direct detection of galactose without going through a complicated derivatization process or using other expensive detectors. At the same time, the charged complex can migrate in an electric field, and the complex formed by α-galactose and β-galactose is separated based on the difference in its electrophoretic mobility and interaction with the capillary silica gel monolithic column stationary phase, without the need to add organic reagents, and environmental protection and saving. The method for separating galactose end group diastereomers provided by the present invention is simple to operate and highly repeatable, and further expands the application of capillary electrochromatography technology in the field of galactose separation.

The invention discloses a nano material synthesized based on a chiral metal organic framework material and a chiral molecularly imprinted polymer, which is characterized in that L-glutamic acid is used as a chiral organic ligand, terephthalic acid is used as an achiral organic ligand, and tetrabutyl titanate is used as a metal salt to prepare the chiral metal organic framework material; the preparation method comprises the following steps: uniformly mixing a dispersion liquid of a chiral metal organic framework material with template molecules, a functional monomer, a cross-linking agent and an initiator, reacting at room temperature, filtering after the reaction is finished, washing an eluent to remove the template molecules, and drying to obtain the nano material. The invention discloses a capillary electrochromatography open tubular column. The capillary electrochromatography open tubular column takes a nano material as a stationary phase. The invention discloses an application of the capillary electrochromatography open tubular column in chiral resolution of DL-histidine. Compared with a CEC open tubular column taking L-Glu / MIL-125 (Ti) or CMIPs as a stationary phase, the chiral separation capability of the CEC open tubular column to the histidine enantiomers is greatly improved.

The current invention provides a novel method to synthesize a mono-disperse non-porous polymer particles with a unique gradient composition from the core to the shell. In particular, the present invention offers the flexibility to design the chemical and physical properties of different sections of the particle. This flexibility allows for significant latitude in the design of particles for analyzing a large variety of samples in different fields—through using these particles in different chromatography techniques including, but not limited to, ion exchange HPLC (e.g., bio-separation at different modes), reversed-phase HPLC, narrow bore and capillary HPLC, hydrophilic / hydrophobic interaction liquid chromatography, capillary electrochromatography separation, and two dimensional liquid chromatography.

The application discloses a polymer modified cyclodextrin MOFs material and a preparation method and application thereof, the material comprises cyclodextrin MOFs and a polymer modified on the cyclodextrin MOFs; the polymer comprises polystyrene-maleic anhydride formed by styrene units and maleic anhydride units. The composite material is good in dispersity, has a particle size in a nanometer scale range and a more concentrated distribution, and is favorable for forming a uniform and stable coating interface. The application constructs an open tube capillary electrochromatography (OT-CEC) / CEC separation system by preparing the composite material into a coating dispersion liquid, introducing into a capillary and forming a coating stationary phase on an inner wall. In a buffer system containing a surfactant SDS, chiral separation of amino acid enantiomer derivatives can be realized. The method is relatively simple in steps, easy to repeat and prepare, has potential chiral analysis and separation application values, and can be used for separation and detection of amino acids and related chiral drug molecules.

The invention relates to the technical field of chemical synthesis and analysis, in particular to a preparation method of a capillary electrochromatography monolithic column of a nano material synthesized based on a chiral metal organic framework and a chiral molecularly imprinted polymer. The preparation method comprises the following steps: synthesizing L-His-ZIF-8 by using zinc nitrate hexahydrate, L-His and 2-MM, then carrying out vinyl modification on the L-His-ZIF-8 by using GMA to obtain Vinyl-L-His-ZIF-8, then preparing a Vinyl-L-His-ZIF-8 dispersion liquid by using the Vinyl-L-His-ZIF-8, rac-DCPP, PG, EDMA and AIBN, filling a vinyl modified capillary with the Vinyl-L-His-ZIF-8 dispersion liquid, and carrying out reaction, elution and blow-drying to obtain the capillary electrochromatography monolithic column. According to the preparation method, the synergistic effect between the L-His-ZIF-8 and the CMIPs which have chiral selection ability is utilized, so that the effect that 1 + 1 is greater than 2 is achieved, and the chiral resolution ability of the capillary monolithic column is greatly improved.

The present application relates to the technical field of drug screening, in particular to an effective mobility ratio screening method based on affinity capillary electrochromatography and application. A target protein is fixed by a MOF material, and a capillary electrochromatography column is prepared by in-situ growth method; a to-be-tested substance is passed through the column, and screening is realized according to the affinity between the to-be-screened component and the protein; the present application adopts effective mobility ratio as a quantitative screening strategy, and the binding affinity between the to-be-screened component and the target protein is directly and quantitatively represented by calculating the effective mobility ratio of the drug in the column containing the fixed phase and the empty capillary column, so that efficient, stable and sensitive screening of multiple active ingredients in a complex system is realized, the stability and reusability of the affinity capillary electrochromatography platform are improved, the drug screening strategy is improved, and the present application is expected to become an effective and reliable strategy for screening of thrombin inhibitors in natural products.

This invention discloses a capillary electrochromatographic column for the separation of chiral tyrosine and its preparation method, belonging to the field of chemical synthesis and analytical technology. It includes a capillary and a composite coating fixed to the inner wall of the capillary. The composite coating comprises chiral metal-organic frameworks (CMOFs) and chiral molecularly imprinted polymers (CMIPs) modified on the surface of the CMOFs. The CMOFs are formed by coordination reaction between MIL-101 (Cr) and L-tyrosine as a chiral ligand. The composite coating (CMIP@CMOF) is a multi-level composite structure formed by in-situ polymerization of the CMOFs as a support in a system containing a crosslinking agent, functional monomer, initiator, and L-tyrosine as a template molecule. The separation system constructed by this coated column exhibits excellent separation performance, with a resolution (Rs) of 5.83 and a selectivity factor (α) of 1.33 for DL-tyrosine enantiomers.

The invention discloses a capillary electrochromatography rapid separation and detection method for acyclovir and guanine, and relates to a rapid separation and detection method for acyclovir and guanine. The invention aims to solve the problems of poor separation effect, short service life and poor reproducibility and stability of the conventional chromatographic column on acyclovir and guanine. The method comprises the following steps: 1, preparing a UiO-66-NH2-coated ZIF-8 bonded open tubular column; and 2, separation of acyclovir and guanine. The invention provides a simple, rapid and efficient method for separating and detecting acyclovir and guanine, namely a UiO-66-NH2-ZIF-8 bonded open tubular column with long service life and good reproducibility and stability is obtained through a simple preparation method, a CEC method for separating and detecting acyclovir and guanine is established, and the lowest concentration of guanine which can be detected is 3.6 mu g / mL.

The invention belongs to the technical field of analytical chemistry, separation science and drug screening, and particularly relates to an open tubular capillary electrochromatography (OT-CEC) screening method based on metal-organic framework materials (MOFs) in-situ encapsulated biomacromolecules and application of the open tubular capillary electrochromatography (OT-CEC) screening method. Introducing a surface active group containing sulfydryl, amino or carboxyl into the inner wall of the capillary tube; a precursor solution containing biomacromolecules is added into the activated capillary tube, so that the biomacromolecules and the surface groups are subjected to adsorption, complexation or covalent binding; a metal ion solution and an organic ligand solution are continuously added into the capillary tube, so that the metal-organic framework material grows in situ on the inner wall of the capillary tube, the biomacromolecule is encapsulated in the MOFs structure, and the biomacromolecule (at) MOFs (at) capillary tube stationary phase is formed; and separating a compound to be detected under the OT-ACEC condition by utilizing the formed biomacromolecule (at) MOFs (at) capillary tube, and screening candidate drugs based on the affinity interaction between the compound to be detected and the biomacromolecule.

The present invention discloses a chiral capillary electrochromatography system based on a DNA nanoflower-modified silica monolithic column and its application, belonging to the field of pharmaceutical analysis. The system uses DNA nanoflowers as chiral selectors, immobilized on a graphene oxide-functionalized capillary silica monolithic column, to separate chiral drugs under electrophoretic conditions. This system can achieve baseline separation of nafopam, propranolol, atenolol, and tyrosine enantiomers. The separation method is simple to operate, establishing a novel, highly selective chiral separation and detection technology.

The invention discloses a nitro-functionalized covalent triazine skeleton nanosheet capillary monolithic column and a preparation method and application thereof.The preparation method includes the steps that a covalent triazine skeleton material CTF is subjected to nitration modification, CTF-NO2 is prepared, nanosheets CTN-NO2 are obtained through ultrasonic stripping, and the nanosheets CTN-NO2 are doped into a polymerization system to construct the capillary monolithic column. A plurality of analysis means are adopted to characterize the prepared material, and it is proved that the material has a stable structure and good thermochemical stability. The monolithic column shows excellent separation performance in capillary electrochromatography separation, and has good selectivity and separation degree. After nitryl is introduced, hydrogen bonds, LP-CH, LP-Pi effects and donor-acceptor effects can be formed, and results show that the monolithic column has efficient selective separation capacity on aromatic compounds and positional isomers, and the recognition and separation capacity on a target object is enhanced; the method suitable for separating and measuring various trace harmful substances in milk powder and fruit cans is established, and the method has a good practical application prospect.