7 results about "Capillary electrochromatography" patented technology

The application discloses a polymer modified cyclodextrin MOFs material and a preparation method and application thereof, the material comprises cyclodextrin MOFs and a polymer modified on the cyclodextrin MOFs; the polymer comprises polystyrene-maleic anhydride formed by styrene units and maleic anhydride units. The composite material is good in dispersity, has a particle size in a nanometer scale range and a more concentrated distribution, and is favorable for forming a uniform and stable coating interface. The application constructs an open tube capillary electrochromatography (OT-CEC) / CEC separation system by preparing the composite material into a coating dispersion liquid, introducing into a capillary and forming a coating stationary phase on an inner wall. In a buffer system containing a surfactant SDS, chiral separation of amino acid enantiomer derivatives can be realized. The method is relatively simple in steps, easy to repeat and prepare, has potential chiral analysis and separation application values, and can be used for separation and detection of amino acids and related chiral drug molecules.

The present application relates to the technical field of drug screening, in particular to an effective mobility ratio screening method based on affinity capillary electrochromatography and application. A target protein is fixed by a MOF material, and a capillary electrochromatography column is prepared by in-situ growth method; a to-be-tested substance is passed through the column, and screening is realized according to the affinity between the to-be-screened component and the protein; the present application adopts effective mobility ratio as a quantitative screening strategy, and the binding affinity between the to-be-screened component and the target protein is directly and quantitatively represented by calculating the effective mobility ratio of the drug in the column containing the fixed phase and the empty capillary column, so that efficient, stable and sensitive screening of multiple active ingredients in a complex system is realized, the stability and reusability of the affinity capillary electrochromatography platform are improved, the drug screening strategy is improved, and the present application is expected to become an effective and reliable strategy for screening of thrombin inhibitors in natural products.

This invention discloses a capillary electrochromatographic column for the separation of chiral tyrosine and its preparation method, belonging to the field of chemical synthesis and analytical technology. It includes a capillary and a composite coating fixed to the inner wall of the capillary. The composite coating comprises chiral metal-organic frameworks (CMOFs) and chiral molecularly imprinted polymers (CMIPs) modified on the surface of the CMOFs. The CMOFs are formed by coordination reaction between MIL-101 (Cr) and L-tyrosine as a chiral ligand. The composite coating (CMIP@CMOF) is a multi-level composite structure formed by in-situ polymerization of the CMOFs as a support in a system containing a crosslinking agent, functional monomer, initiator, and L-tyrosine as a template molecule. The separation system constructed by this coated column exhibits excellent separation performance, with a resolution (Rs) of 5.83 and a selectivity factor (α) of 1.33 for DL-tyrosine enantiomers.

The invention discloses a capillary electrochromatography rapid separation and detection method for acyclovir and guanine, and relates to a rapid separation and detection method for acyclovir and guanine. The invention aims to solve the problems of poor separation effect, short service life and poor reproducibility and stability of the conventional chromatographic column on acyclovir and guanine. The method comprises the following steps: 1, preparing a UiO-66-NH2-coated ZIF-8 bonded open tubular column; and 2, separation of acyclovir and guanine. The invention provides a simple, rapid and efficient method for separating and detecting acyclovir and guanine, namely a UiO-66-NH2-ZIF-8 bonded open tubular column with long service life and good reproducibility and stability is obtained through a simple preparation method, a CEC method for separating and detecting acyclovir and guanine is established, and the lowest concentration of guanine which can be detected is 3.6 mu g / mL.

The invention belongs to the technical field of analytical chemistry, separation science and drug screening, and particularly relates to an open tubular capillary electrochromatography (OT-CEC) screening method based on metal-organic framework materials (MOFs) in-situ encapsulated biomacromolecules and application of the open tubular capillary electrochromatography (OT-CEC) screening method. Introducing a surface active group containing sulfydryl, amino or carboxyl into the inner wall of the capillary tube; a precursor solution containing biomacromolecules is added into the activated capillary tube, so that the biomacromolecules and the surface groups are subjected to adsorption, complexation or covalent binding; a metal ion solution and an organic ligand solution are continuously added into the capillary tube, so that the metal-organic framework material grows in situ on the inner wall of the capillary tube, the biomacromolecule is encapsulated in the MOFs structure, and the biomacromolecule (at) MOFs (at) capillary tube stationary phase is formed; and separating a compound to be detected under the OT-ACEC condition by utilizing the formed biomacromolecule (at) MOFs (at) capillary tube, and screening candidate drugs based on the affinity interaction between the compound to be detected and the biomacromolecule.

The present invention discloses a chiral capillary electrochromatography system based on a DNA nanoflower-modified silica monolithic column and its application, belonging to the field of pharmaceutical analysis. The system uses DNA nanoflowers as chiral selectors, immobilized on a graphene oxide-functionalized capillary silica monolithic column, to separate chiral drugs under electrophoretic conditions. This system can achieve baseline separation of nafopam, propranolol, atenolol, and tyrosine enantiomers. The separation method is simple to operate, establishing a novel, highly selective chiral separation and detection technology.

The invention discloses a nitro-functionalized covalent triazine skeleton nanosheet capillary monolithic column and a preparation method and application thereof.The preparation method includes the steps that a covalent triazine skeleton material CTF is subjected to nitration modification, CTF-NO2 is prepared, nanosheets CTN-NO2 are obtained through ultrasonic stripping, and the nanosheets CTN-NO2 are doped into a polymerization system to construct the capillary monolithic column. A plurality of analysis means are adopted to characterize the prepared material, and it is proved that the material has a stable structure and good thermochemical stability. The monolithic column shows excellent separation performance in capillary electrochromatography separation, and has good selectivity and separation degree. After nitryl is introduced, hydrogen bonds, LP-CH, LP-Pi effects and donor-acceptor effects can be formed, and results show that the monolithic column has efficient selective separation capacity on aromatic compounds and positional isomers, and the recognition and separation capacity on a target object is enhanced; the method suitable for separating and measuring various trace harmful substances in milk powder and fruit cans is established, and the method has a good practical application prospect.