4 results about "Capillary electrochromatography" patented technology

The application discloses a polymer modified cyclodextrin MOFs material and a preparation method and application thereof, the material comprises cyclodextrin MOFs and a polymer modified on the cyclodextrin MOFs; the polymer comprises polystyrene-maleic anhydride formed by styrene units and maleic anhydride units. The composite material is good in dispersity, has a particle size in a nanometer scale range and a more concentrated distribution, and is favorable for forming a uniform and stable coating interface. The application constructs an open tube capillary electrochromatography (OT-CEC) / CEC separation system by preparing the composite material into a coating dispersion liquid, introducing into a capillary and forming a coating stationary phase on an inner wall. In a buffer system containing a surfactant SDS, chiral separation of amino acid enantiomer derivatives can be realized. The method is relatively simple in steps, easy to repeat and prepare, has potential chiral analysis and separation application values, and can be used for separation and detection of amino acids and related chiral drug molecules.

The present application relates to the technical field of drug screening, in particular to an effective mobility ratio screening method based on affinity capillary electrochromatography and application. A target protein is fixed by a MOF material, and a capillary electrochromatography column is prepared by in-situ growth method; a to-be-tested substance is passed through the column, and screening is realized according to the affinity between the to-be-screened component and the protein; the present application adopts effective mobility ratio as a quantitative screening strategy, and the binding affinity between the to-be-screened component and the target protein is directly and quantitatively represented by calculating the effective mobility ratio of the drug in the column containing the fixed phase and the empty capillary column, so that efficient, stable and sensitive screening of multiple active ingredients in a complex system is realized, the stability and reusability of the affinity capillary electrochromatography platform are improved, the drug screening strategy is improved, and the present application is expected to become an effective and reliable strategy for screening of thrombin inhibitors in natural products.

This invention discloses a capillary electrochromatographic column for the separation of chiral tyrosine and its preparation method, belonging to the field of chemical synthesis and analytical technology. It includes a capillary and a composite coating fixed to the inner wall of the capillary. The composite coating comprises chiral metal-organic frameworks (CMOFs) and chiral molecularly imprinted polymers (CMIPs) modified on the surface of the CMOFs. The CMOFs are formed by coordination reaction between MIL-101 (Cr) and L-tyrosine as a chiral ligand. The composite coating (CMIP@CMOF) is a multi-level composite structure formed by in-situ polymerization of the CMOFs as a support in a system containing a crosslinking agent, functional monomer, initiator, and L-tyrosine as a template molecule. The separation system constructed by this coated column exhibits excellent separation performance, with a resolution (Rs) of 5.83 and a selectivity factor (α) of 1.33 for DL-tyrosine enantiomers.

The invention belongs to the technical field of analytical chemistry, separation science and drug screening, and particularly relates to an open tubular capillary electrochromatography (OT-CEC) screening method based on metal-organic framework materials (MOFs) in-situ encapsulated biomacromolecules and application of the open tubular capillary electrochromatography (OT-CEC) screening method. Introducing a surface active group containing sulfydryl, amino or carboxyl into the inner wall of the capillary tube; a precursor solution containing biomacromolecules is added into the activated capillary tube, so that the biomacromolecules and the surface groups are subjected to adsorption, complexation or covalent binding; a metal ion solution and an organic ligand solution are continuously added into the capillary tube, so that the metal-organic framework material grows in situ on the inner wall of the capillary tube, the biomacromolecule is encapsulated in the MOFs structure, and the biomacromolecule (at) MOFs (at) capillary tube stationary phase is formed; and separating a compound to be detected under the OT-ACEC condition by utilizing the formed biomacromolecule (at) MOFs (at) capillary tube, and screening candidate drugs based on the affinity interaction between the compound to be detected and the biomacromolecule.