The invention provides a graphene oxide bonding capillary electrochromatography monolithic column. A preparing method of the graphene oxide bonding capillary electrochromatography monolithic column comprises the steps that GO and DMF are mixed and subjected to ultrasonic dispersion, then, pAS and DCC are added, reaction is conducted at the temperature of 40-80 DEG C under the argon protection condition in dark in a stirring mode for 12-48 h, reaction liquid is filtered, and pAS-GO is obtained; the pAS-GO is mixed with cyclohexanol and subjected to ultrasonic dispersion, then, styrene, DVB, methylbenzene, a radical initiator and AMPS are added, ultrasonic dispersion continues to be conducted, nitrogen is injected into the mixture, and reaction mixed liquid is obtained; the obtained reaction mixed liquid is introduced into a position of a preset stationary phase in a preprocessed capillary tube, then, the two ends of the capillary tube are sealed through silica gel, the capillary tube is put at the temperature of 40-100 DEG C to be subjected to curing reaction for 6-24 h, post-processing is conducted on the capillary tube, and the graphene oxide bonding capillary electrochromatography monolithic column is prepared. The reaction process is simple, the number of steps is small, reaction conditions are controllable, and the preparation effect is more ideal.