32 results about "Hydroxyapatite crystal" patented technology

The invention relates to the technical field of water treatment, and particularly discloses a crystal nucleus granulation induced hardness removal integrated water treatment process which comprises the following steps: firstly, ultrasonically dispersing nano-hydroxyapatite in deionized water, adding gamma-glycidoxypropyltrimethoxysilane for reaction, and treating to obtain a silanized hydroxyapatite crystal nucleus; the sodium polyacrylate reacts with aluminum sulfate and the like in an ethylene glycol solution to prepare the composite inducer. The preparation method comprises the following steps: reacting the two components in a phosphate buffer solution in proportion to obtain inducer-loaded crystal nucleus particles, carrying out multi-step reaction and treatment on the inducer-loaded crystal nucleus particles with calcium chloride and the like to obtain surface-coated crystal nucleus granulation bodies, and mixing the surface-coated crystal nucleus granulation bodies with cationic polyacrylamide and nano-zinc oxide to prepare the reinforced hardness removal agent. When to-be-treated water is treated, the reinforced hardness removal agent is added, stirring and precipitation are performed, supernate sequentially passes through the activated carbon filter column to adsorb impurities and is sterilized by the ultraviolet disinfection device, and finally effluent is collected, so that the hardness in the water can be efficiently removed, the process integration level is high, and the effect is good.

The invention discloses a grading reaction crystallization recovery process and system for semiconductor fluorine-containing and phosphorus-containing waste liquid, the fluorine-containing and phosphorus-containing waste liquid and a lime solution react in a fluorine recovery crystallization reactor under the condition that the pH is 3-4 to form calcium fluoride crystals, and the calcium fluoride crystals are dehydrated to obtain calcium fluoride particle crystal products; the effluent of the fluorine recovery crystallization reactor, the calcium fluoride crystal dehydration filtrate, the lime solution and the calcium chloride solution react in a secondary reaction tank under the condition that the pH is 5-6, and calcium fluoride, fluorapatite, silicon dioxide and silicon-aluminum precipitates are removed through coagulation, flocculation and precipitation; effluent of the second-stage reaction tank, the lime solution and the calcium chloride solution react in the phosphorus recovery crystallization reactor under the condition that the pH is 7-8 to generate hydroxyapatite crystals, and the hydroxyapatite crystals are dehydrated to obtain hydroxyl calcium phosphate particle crystal products. Meanwhile, a high-purity calcium fluoride crystal product and a hydroxyl calcium phosphate crystal product are produced, and fluorine and phosphorus are recycled.

The invention provides a tooth patch for repairing tooth hardness and a preparation method thereof, the tooth patch comprises a support material and gel, and the gel comprises the following components by mass: 1.5-2 parts of regenerated silicon, 0.5-0.7 part of A200, 1.5-2.5 parts of a thickener, 15-20 parts of a film-forming agent, 4-6 parts of a humectant, and 1-1.5 parts of a gelling agent. The tooth paste can form a hydroxyapatite layer on the tooth surface, repair tooth enamel, promote remineralization of the tooth surface and improve tooth hardness, is alkalescent, and can prevent dental caries; after contacting with saliva, hydroxyapatite crystals can be quickly formed in dentinal tubules to block the dentinal tubules, and the hydrogel can be used for treating tooth sensitivity.

The invention relates to a breast cancer dynamic risk assessment method based on an adaptive model, and aims to improve the accuracy of early screening and risk prediction of breast cancer. The method comprises the following steps that firstly, clinical data of the unresected side mammary gland of an estrogen receptor positive breast cancer patient are obtained, and the data comprise the estrogen receptor state, the hormone treatment duration and mammary gland density classification; selecting an imaging detection mode according to the mammary gland density grading and the estrogen receptor state, and obtaining a calcification focus image of the target mammary gland; performing multi-parameter quantitative analysis on the image, and extracting parameters such as hydroxyapatite crystal proportion, calcification edge sharpness index and the like; and carrying out risk value calculation by using the trained adaptive risk assessment model, comparing the risk value with a preset risk grading threshold, and finally generating an assessment report based on a risk grade. According to the method, accurate dynamic risk assessment can be provided for the breast cancer patient, and personalized health management and intervention decision are supported.

The invention discloses demineralized dentin remineralization mouthwash based on cp20k-p3 bionic polypeptide and an application of the demineralized dentin remineralization mouthwash. The mouthwash is prepared by mixing the polypeptide cp20k-p3 (the amino acid sequence is CNQKHPCWRRHGKKHRGLHRKFHGNACNC) derived from barnacle mucin 20K, an aqueous solution of calcium salt and an aqueous solution of phosphate. The polypeptide can specifically recognize and adsorb an organic matrix exposed on the surface of demineralized dentin, guide hydroxyapatite crystals to directionally deposit on the demineralized part of the dentin, and form a compact mineralized layer similar to a natural structure on the surface of the dentin. Compared with a calcium phosphate mixed aqueous solution without cp20k-p3, the preparation method is simple and convenient to operate, can form a compact mineralized layer with the surface of the re-demineralized dentin, and has excellent stability in an oral environment.

The application provides a method and application for rapidly preparing hydroxyapatite material, and belongs to the field of inorganic material synthesis. The application uses a calcium source solution containing glacial acetic acid and a phosphorus source solution containing hydroxyapatite crystal seeds and sugar alcohol solid as raw materials, uses micro-channel continuous flow technology and optimizes reaction parameters to rapidly prepare a crude hydroxyapatite, and then uses a joule heat treatment rapid high-low temperature technology to rapidly prepare a microspherical hydroxyapatite material, so that the preparation time is greatly shortened. The particle size range, specific surface area and pore size range of the material are 528-1053 nm, 95.72-117.71 m 2 / g and 0.5-83 nm respectively, which indicates that the material is a multi-level pore structure of micron level, high specific surface area, micropore and mesopore and macropore, can realize adsorption of organic matter-containing wastewater, the removal rate is more than 97.5%, is 1.87 times of the removal capacity of hydroxyapatite material without joule heat treatment, is outstanding in the field of organic matter adsorption and has good industrialization prospect.

The invention discloses an enamel secretion prolonging and thickness increasing method based on KLK4 alpha protein, and relates to the technical field of biomedicine and dental materials.According to the method, expression of the KLK4 alpha protein is specifically regulated and controlled, forward intervention in the secretion period in the enamel development process is achieved, synthesis and accumulation of enamel matrix protein are directly promoted, and the effect of prolonging the secretion period of the enamel is achieved. A recombinant lentiviral vector mediated gene transduction technology is utilized to specifically introduce the KLK4alpha gene into a dental embryo glazing cell and realize overexpression, and the expressed KLK4alpha protein remarkably delays the process of transformation from a secretion period to a mature period by regulating the secretion activity of the glazing cell, so that the expression level of the KLK4alpha protein is improved, and the expression level of the KLK4alpha protein is improved. The time sequence intervention effectively prolongs the secretion window period of key organic matrix components such as glaze protein, glaze protein and the like, so that before mineralization is started, the total amount of extracellular matrix net racks is substantially increased, a space is provided for nucleation of hydroxyapatite crystals, and finally, the thickness of a mineral phase is remarkably increased; and the protection performance of the dental crown structure is enhanced.

The invention belongs to the technical field of biomedical materials, and particularly relates to biomimetic mineralized II-type collagen hydrogel as well as a preparation method and application thereof. The method comprises the following steps: gelling an acid solution of type II collagen in an alkaline environment to obtain type II collagen hydrogel; and dipping the type II collagen hydrogel in a mineralizing solution for mineralization to obtain the biomimetic mineralized type II collagen hydrogel. According to the method provided by the invention, the complex shape and nano-scale characteristics of the collagen fiber template can be accurately filled and copied, and the obtained biomimetic mineralized II-type collagen hydrogel can realize a tissue bionic effect. The biomimetic mineralized gel prepared by the invention has type I and type II amido bonds, the unmineralized collagenous fiber has a 67nm D-banding periodic structure, and hydroxyapatite crystals in the mineralized collagenous fiber are distributed along a long axis of collagen fibril composed of type II collagen, so that mineralization in the collagenous fiber is realized.

The invention provides a method and application of a hydroxyapatite and silver composite coating modified antibacterial film, and belongs to the technical field of material modification. According to the preparation method, silver elementary substances and hydroxyapatite crystals are used as sputtering sources, in the inert gas atmosphere, the hydroxyapatite and silver composite coating is prepared on the surface of a PVDF bone electroactive square film through the magnetron sputtering technology, and the antibacterial promoting film modified by the hydroxyapatite and silver composite coating is obtained. The hydroxyapatite and silver composite coating modified antibacterial promoting film has excellent biocompatibility, antibacterial performance and osteogenesis inducing activity, and the problems that in the prior art, a PVDF bone electroactive film is poor in osseointegration and insufficient in bone healing speed and effectiveness are effectively solved; the hydroxyapatite and silver composite coating modified antibacterial film is suitable for the aspects of tissue engineering, bone defect repair and the like, and the application scene of the PVDF bone electroactive film in the oral cavity and other parts is expanded.

The invention discloses a biomimetic polypeptide cp20k-p3 for inducing remineralization of demineralized dentin and application of the biomimetic polypeptide cp20k-p3, the biomimetic polypeptide disclosed by the invention is a polypeptide cp20k-p3 derived from barnacle gum protein 20K, and the amino acid sequence of the biomimetic polypeptide cp20k-p3 is CNQKHPCWRRHGKKHGLHRKFHGNACNC. According to the bionic polypeptide disclosed by the invention, amorphous calcium phosphate can be rapidly generated in simulated body fluid and can be well adsorbed on the surface of a demineralized dentin matrix, and newly generated hydroxyapatite crystals can deeply seal exposed demineralized dentin tubules. A brand new biomineralization polypeptide is provided for bone demineralization dentin repair.

The invention belongs to the field of biomedicine, and relates to application of Herbacetin in prevention of renal nipple calcium spot related calcium oxalate kidney stone. The invention relates to an application of Herbacetin or pharmaceutically acceptable salt, ester, solvate, crystal form or prodrug thereof in preparation of a reagent for preventing renal papillary calcium spot related calcium oxalate kidney stone, the Herbacetin has the following structural formula: the Herbacetin is used for preventing and treating renal interstitial calcium salt deposition and Randall plaque progress for the first time, and experiments prove that the Herbacetin can be used for preventing and treating renal interstitial calcium salt deposition and Randall plaque progress. Herbacetin has the effect of inhibiting the inflammation induction of renal interstitial hydroxyapatite crystals on fibroblasts, and has the application prospect of improving the immune microenvironment of renal interstitial and the deposition of renal interstitial calcium salt.

The invention provides a method for rapidly preparing a hydroxyapatite material and application, and belongs to the field of inorganic material synthesis. According to the method, a calcium source solution containing glacial acetic acid and a phosphorus source solution containing hydroxyapatite seed crystals and sugar alcohol solids are used as raw materials, a micro-channel continuous flow technology is utilized, reaction parameters are optimized, crude hydroxyapatite is rapidly prepared, then a rapid high-low temperature technology of Joule heat treatment is adopted, and the hydroxyapatite is prepared. The rapid preparation of the microspherical hydroxyapatite material is realized, and the preparation time is greatly shortened. The particle size range, the specific surface area and the aperture range of the material are 528-1053 nm, 95.72-117.71 m < 2 > / g and 0.5-83 nm respectively, which indicates that the material is of a micron-scale, high-specific-surface-area, microporous, mesoporous and macroporous hierarchical pore structure, can realize adsorption of wastewater containing organic matters, has a removal rate of 97.5% or above, which is 1.87 times of the removal capacity of a non-joule heat-treated hydroxyapatite material, and has a good application prospect. The method has outstanding performance in the field of organic matter adsorption and has good industrialization prospects.

This invention relates to the field of biomaterials technology, providing a composite material for biomimetic repair of dental hard tissues, its preparation method, and its application. The preparation method of the composite material includes the following steps: mixing a carboxybetaine-phosphonic acid copolymer with a calcium salt to obtain solution A; adding an aqueous phosphate solution to solution A to carry out an in-situ reaction to obtain the composite material for biomimetic repair of dental hard tissues. In the composite material provided by this application, the carboxybetaine-phosphonic acid copolymer, under the action of calcium phosphate ions, can form an amelogenin-like nanoribbon structure, simultaneously driving amorphous calcium phosphate to undergo supramolecular self-assembly, constructing a composite material conforming to the "self-assembly-mineralization" synergistic mode. This composite material can directionally deposit dense enamel-like enamel pillar structures of hydroxyapatite crystals on the surface of demineralized enamel, efficiently inducing oriented structural regeneration of damaged enamel and significantly restoring the mechanical properties of demineralized enamel.

The present invention relates to the technical field of biomedical materials, and specifically to a preparation method of a composite hydroxyapatite filler for injection. A preparation method of a composite hydroxyapatite filler for injection includes the following steps: preparation of fluorine-containing collagen; preparation of unidirectional nano-hydroxyapatite composite material; preparation of anti-inflammatory extract solution; mixing preparation and treatment of injectable hydrogel. By placing the pre-frozen solution in a strong magnetic field for reaction, collagen fibers and hydroxyapatite crystals arranged orderly are initially formed under the action of the strong magnetic field, and then unidirectional freezing from bottom to top of the reaction system and application of a strong magnetic field are carried out by liquid nitrogen, so that the internal structure of the unidirectional nano-hydroxyapatite composite material is more compact and stable, with better anti-swelling performance and higher anti-compression strength. During the process of bone defect repair, it can have excellent space maintenance ability and enhance the restoration effect of the external contour of the bone defect.

The invention relates to the technical field of materials, in particular to a hydrothermal reaction system of nanofibers as well as a preparation method and application of the hydrothermal reaction system. The reaction system comprises: a calcium source containing calcium ions; a phosphorus source containing phosphorus ions; a doping source containing lanthanum ions; the invention discloses a structure-directing agent capable of being selectively adsorbed on a hydroxyapatite crystal face under an alkaline condition and inducing one-dimensional directional growth. And an alkaline regulator. According to the preparation method disclosed by the invention, crystallization growth is directly carried out under a hydrothermal condition, and a high-temperature calcination step is not needed, so that the problems of fiber breakage, fusion and morphology collapse caused by macromolecular template decomposition and rapid crystal sintering are fundamentally avoided. The prepared nanofiber has ideal morphology with good continuity, uniform diameter and high length-diameter ratio, and can simulate the microstructure of natural collagen fiber more truly, thereby providing a more excellent biological microenvironment for cells.

The application provides a method for modifying an antibacterial film with a hydroxyapatite and silver composite coating and application, and belongs to the technical field of material modification. The application takes silver single element and hydroxyapatite crystals as sputtering sources, and prepares a hydroxyapatite and silver composite coating on the surface of a PVDF bone electroactive square film through a magnetron sputtering technology in an inert gas atmosphere to obtain an antibacterial film modified with the hydroxyapatite and silver composite coating. The antibacterial film modified with the hydroxyapatite and silver composite coating has superior biocompatibility, antibacterial performance and osteogenesis induction activity, and effectively solves the problems of poor bone integration, bone healing speed and effectiveness of the PVDF bone electroactive film in the prior art. The antibacterial film modified with the hydroxyapatite and silver composite coating is suitable for tissue engineering and bone defect repair, and expands the application scenarios of the PVDF bone electroactive film in the oral cavity and other parts.

This invention discloses a method, apparatus and application for nitrogen and phosphorus removal from anaerobic ammonia oxidation granular sludge. The method includes: (1) raw biogas slurry is fed into three separate locations to a primary pre-anoxic tank, an anaerobic tank and an integrated anaerobic ammonia oxidation reactor; the primary pre-anoxic tank is also fed with recycled sludge; (2) effluent from the anaerobic tank is fed into a primary sedimentation tank, the supernatant from the primary sedimentation tank is fed into the integrated anaerobic ammonia oxidation reactor, and the bottom sludge is fed into a secondary anoxic tank by a sludge pump; (3) calcium salt is added to the integrated anaerobic ammonia oxidation reactor to form hydroxyapatite crystal nuclei to accelerate the formation of anaerobic ammonia oxidation granular sludge, and alkali solution is added to control the pH value in the reactor to 7.5-8.3; (4) effluent from the integrated anaerobic ammonia oxidation reactor is fed into a secondary anoxic tank, the bottom of the secondary anoxic tank is connected to the aerobic tank; effluent from the aerobic tank is fed into a secondary sedimentation tank, the supernatant from the secondary sedimentation tank is discharged after meeting the standards, and the bottom sludge is recycled back to the primary pre-anoxic tank.

The invention provides a self-assembled polypeptide material for bionic remineralization of demineralized enamel and application of the self-assembled polypeptide material, and belongs to the technical field of remineralization repair of demineralized enamel. The invention provides a self-assembled polypeptide material for bionic remineralization of demineralized enamel. The self-assembled polypeptide material comprises a self-assembled template unit, an enzyme digestion response unit and a bonding unit which are connected in sequence, the amino acid sequence of the self-assembly template unit is GYINFpSYE; the amino acid sequence of the enzyme digestion response unit is GPQGIWGQ; and the amino acid sequence of the bonding unit is DKTKREEVD. The self-assembled polypeptide material provided by the invention simultaneously realizes a template effect of an organic matter pre-organization, guides directional deposition of calcium and phosphorus ions, slowly degrades the organic matter, reduces organic occupation, and promotes dual effects of directional arrangement and phase change crystallization of amorphous calcium phosphate; the purposes of regularly and orderly arranged hydroxyapatite crystal formation, demineralized enamel structure repair and mechanical property recovery are achieved.

Disclosed are titanium compositions and methods of manufacturing titanium compositions that comprise an oxide coating formed via an anodization process. An anodization electrolyte used in the anodization process comprises (1) a chelating acid component, (2) a phosphate component, and (3) a phosphate binder component. The resulting oxide coating comprises α-tricalcium phosphate and / or hydroxyapatite crystalline compounds with a calcium to phosphate (Ca / P) ratio within a range of human bone.

The application provides a method for preparing hydroxyapatite with different morphologies from solid waste by using ammonia alkali method, and belongs to the field of comprehensive utilization of solid waste resources and synthesis of inorganic materials. First, ammonia distillation waste supernatant, alkali residue, acidification aid and functional non-imidazole liquid of the solid waste of ammonia alkali method are mixed, then concentrated hydrochloric acid is added, then calcium-containing alkaline solid material is added to adjust pH, remove impurities and concentrate, then hydroxyapatite crystal formation inducer is added, finally, the hydroxyapatite is obtained after reaction with trisalt phosphate solution and cleaning and purification, and the hydroxyapatite has high yield, high purity and different morphologies, including flaky, tubular and spherical morphologies. The utilization rate of the alkali residue reaches 79.05-82.45%, the extraction of effective components reaches more than 93%, the yield of the hydroxyapatite is as high as more than 99.9%, and the purity is more than 99%, so that not only the emission of the alkali residue is reduced, but also the ammonia distillation waste supernatant is reused.

This invention provides the application of a TPE-Ketoalkyne probe in the preparation of a product specifically distinguishing urate and hydroxyapatite crystals. This product comprises a TPE-Ketoalkyne probe that specifically binds to urate crystals. This invention optimizes rapid imaging technology for MSU crystals in in vitro studies using the TPE-Ketoalkyne probe. The imaging effect is comparable to that of birefringent MSU crystals detected by clinical professionals using CPLM, especially in differentiating between different types of crystalline arthritis caused by MSU and HAP crystals. The fluorescence produced by the TPE-Ketoalkyne probe differs from ordinary fluorescence; it is non-quenchable and can be monitored for extended periods. This provides not only a good platform for basic research related to gout but also a new auxiliary means for clinical detection of gout.

The invention discloses a process and a system for fractional precipitation and resource recovery of wastewater containing phosphorus and aluminum, which are characterized in that the wastewater containing phosphorus and aluminum collected in a wastewater collection tank and a lime solution in a lime agent tank are respectively pumped into a pretreatment reaction tank, aluminum in the wastewater forms an aluminum phosphate precipitate under the condition that the pH is 5-6, and the aluminum phosphate precipitate enters a precipitation tank for mud-water separation; water is fed into a phosphorus recovery crystallization reactor, a calcium chloride agent pool and a lime agent pool are combined to add agents into the phosphorus recovery crystallization reactor, a crystallization reaction is carried out under the condition that the pH value is 7-8 to obtain hydroxyapatite crystals, and sludge separated from a sedimentation tank and the hydroxyapatite crystals are subjected to cleaning and dewatering treatment through a first cleaning and dewatering system and a second cleaning and dewatering system respectively; according to the method, efficient recovery of phosphorus and aluminum is achieved, the landfill problem of phosphorus-containing and aluminum-containing chemical sludge is avoided, and the pressure of natural resources is relieved.

The application relates to the field of fluorine pollution treatment, in particular to a preparation method of hydroxyapatite and application of the hydroxyapatite in fluorine removal of mine water, and solves the problem that the adsorption effect of the existing fluorine removal agent is still poor, thereby limiting the wide application of the fluorine removal agent in fluorine wastewater. In the preparation process of the fluorine removal agent, the hydroxyapatite is loaded on lanthanum-doped porous nanofibers, the adsorption active sites on the surface of the hydroxyapatite are increased through the electrostatic attraction or ion exchange of metal ions or metal oxides on the electrically negative pollutants, the adsorption effect on fluorine ions is improved, the cetyltrimethylammonium bromide changes the surface charge by embedding into the hydroxyapatite crystal lattice, the surface of the hydroxyapatite shows positive charge, the electrostatic action between the hydroxyapatite and F ‑ is strengthened, a large number of amino functional groups are contained on the chitosan, the fluorine ions can be reacted with the fluorine ions, the fluorine ions in water can be adsorbed on the surface through adsorption, and the fluorine removal purpose is achieved.

This application discloses a nerve conduit, comprising: mineralized collagen nanofibers doped with active elements; the mineralized collagen nanofibers containing hydroxyapatite crystals; the calcium element in the hydroxyapatite crystal lattice being replaced by a cation containing the active element, and / or, the phosphate group in the hydroxyapatite crystal lattice being replaced by anion containing the active element; the nerve conduit provided by this application, by introducing dopant elements, allows the cations or anions of the dopant elements to be effectively and controllably released from the phosphorus or calcium in the hydroxyapatite lattice of the mineralized collagen nanofibers, thereby effectively promoting the regeneration of human tissues such as nerves and blood vessels compared to nerve conduits without dopant elements.

The present invention pertains to a surface-treated titanium base material that has a stratified nano-rough surface and is characterized in that: the stratified nano-rough surface is formed by including an amorphous titanium oxide; the average distribution density of nano-projections forming the stratified nano-rough surface is 20-50 projections / µm2; and the distribution anisotropy of the nano-projections is 0.70-1.20. The present invention also pertains to: a method for manufacturing the surface-treated titanium base material; a composite; a culture bed; and a method for manufacturing acicular nano-crystals of hydroxyapatite.

Disclosed are titanium compositions and methods of manufacturing titanium compositions that comprise an oxide coating formed via an anodization process. An anodization electrolyte used in the anodization process comprises (1) a chelating acid component, (2) a phosphate component, and (3) a phosphate binder component. The resulting oxide coating comprises α-tricalcium phosphate and / or hydroxyapatite crystalline compounds with a calcium to phosphate (Ca / P) ratio within a range of human bone.

The highly mimic natural HAP scaffolds are very important for improving the bone regeneration efficiency more than the commercial natural hydroxyapatite due to restoring of naturally occurring trace elements because the calcination process does not exceed 550°c so the produced natural hydroxyapatite mimic the biological appetite which cause substitution of foreign ions and vacancies into the hydroxyapatite crystals structures are common.

The invention provides a hydroxyapatite composite nanomaterial, preparation thereof and application of the hydroxyapatite composite nanomaterial in antibacterial and antibacterial bone repair drugs. The preparation method of the composite nano material comprises the following steps: fully dissolving calcium nitrate tetrahydrate and polyacrylic acid in deionized water, and adjusting the pH value to 9-10 to obtain a viscous solution; adding a diammonium hydrogen phosphate aqueous solution, and reacting to obtain a suspension; dialyzing the suspension liquid to obtain hydroxyapatite dispersion liquid; and adding a mixed solution of a carboxyl activator, a carboxyl condensing agent, alkali and phthalocyanine, reacting, dialyzing and drying. According to the invention, polyacrylic acid is selected as a polymer medium to help hydroxyapatite crystals to grow and assemble into spherical nanoparticles and prevent aggregation of the spherical nanoparticles, and silicon phthalocyanine is further used for modification to obtain multifunctional nanoparticles, so that the stability and dispersity of the system are improved, and the system has photodynamic therapy and photothermal therapy properties, and has good application prospects. The phototherapy antibacterial bone repair medicine is a novel bone repair medicine with a phototherapy antibacterial effect, can effectively promote osteogenesis and has an excellent antibacterial capability.

The invention relates to the field of oral care, and discloses an oral composition as well as application and a use method thereof. The oral composition contains chondroitin sulfate, calcium salt and phosphorus salt, on the basis of the total mass of the oral composition, the content of the chondroitin sulfate is 0.05-2%, the content of the calcium salt is 0.1-0.3%, and the content of the phosphorus salt is 0.1-0.3%. The oral composition can significantly improve the quality of generated hydroxyapatite crystals, realizes deep and lasting sealing, enhances the acid erosion resistance, improves the remineralization efficiency, and has a significant market prospect.

The invention discloses mineralized collagen microspheres as well as a preparation method and application thereof. According to the preparation method, the collagen microspheres are prepared through an improved emulsification speed-controlled cross-linking method, so that the prepared microspheres are more concentrated in particle size distribution, more regular in morphology, more uniform in cross-linking degree and higher in mechanical strength; and tightly combining hydroxyapatite crystals with the prepared collagen microspheres by adopting an in-situ mineralization technology to obtain the mineralized collagen microspheres. Through in-situ mineralization, the mechanical strength of the collagen microspheres is enhanced, the obtained mineralized collagen microspheres are high in particle size uniformity and concentrated in size, richer communicated pore structures are formed through accumulation of the microspheres, permeation and attachment of cells are effectively promoted, and the bone repair process is accelerated.