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Preparation method for regenerating dental prosthetic material and acidic amino acid-induced demineralized dental enamel outer enamel prism thereof in situ

An acidic amino acid, repair material technology, applied in dental preparations, dental prostheses, dentistry, etc., can solve the problems of enamel to be negotiated, inconvenient for wide application, small molecular weight of peptides, etc., to reduce the harsh application environment and eliminate crack tip stress. , the effect of uniform crystal morphology

Active Publication Date: 2014-08-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A layer of dense mineral deposits is formed, but the phage surface display technology is used to screen out the polypeptides that can specifically bind to the axial direction of the enamel surface. The process is expensive and takes a long time, so it is not suitable for screening
Inconvenience for widespread application
[0012] 7. CN103408653A invention discloses a bionic anti-caries polypeptide and its preparation method and application. The polypeptide has a small molecular weight, which can improve the adsorption on the surface of tooth enamel, and then better play its role, and promote early demineralization of tooth enamel (early caries) remineralization, the patent provides a synthetic method of peptides, but the specimens used in the experiment are bovine teeth, and the application of human enamel remains to be discussed
At present, although there are a few reports on the patent research on the remineralization of the demineralized enamel surface with independent amino acids that have a strong adsorption effect on calcium ions, the method for regenerating enamel-like mineral deposits on the enamel surface is still rare. However, under the conditions of suitable temperature, time, suitable amino acid concentration and species, the case of continuous growth along the original growth direction of natural enamel enamel column has not been reported yet.

Method used

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  • Preparation method for regenerating dental prosthetic material and acidic amino acid-induced demineralized dental enamel outer enamel prism thereof in situ
  • Preparation method for regenerating dental prosthetic material and acidic amino acid-induced demineralized dental enamel outer enamel prism thereof in situ
  • Preparation method for regenerating dental prosthetic material and acidic amino acid-induced demineralized dental enamel outer enamel prism thereof in situ

Examples

Experimental program
Comparison scheme
Effect test

comparative example 1

[0059] (1) Preparation and processing of human enamel specimens

[0060] ① Cut the enamel after removing soft scale, calculus and soft tissue into 0.5×0.5×0.2cm 3 Enamel slices of different sizes are polished and polished in sequence with 360 mesh, 400 mesh, 600 mesh, 800 mesh, 1000 mesh, 1200 mesh, and 2000 mesh abrasive paper.

[0061] ②Put the enamel slices in a small beaker, add 75wt% absolute ethanol, the liquid surface is not over the enamel slices, shake the beaker to make the enamel slices fully contact with ethanol, and then put them into an ultrasonic cleaning machine for ultrasonic cleaning for 15 minutes; take out Pour out the absolute ethanol from the beaker, rinse the enamel slices repeatedly with deionized water; then add deionized water until the liquid surface covers the enamel slices, ultrasonically clean and vibrate for 15 minutes, take out the enamel slices, and dry them.

[0062] ③ The enamel slices were soaked in 37% phosphoric acid solution for 60s. Th...

Embodiment 1

[0078] (1) Preparation and processing of human enamel specimens

[0079] ① Cut the enamel after removing soft scale, calculus and soft tissue into 0.5×0.5×0.2cm 3 Enamel slices of different sizes are polished and polished in sequence with 360 mesh, 400 mesh, 600 mesh, 800 mesh, 1000 mesh, 1200 mesh, and 2000 mesh abrasive paper.

[0080] ②Put the enamel slices in a small beaker, add 75wt% absolute ethanol, the liquid surface is not over the enamel slices, shake the beaker to make the enamel slices fully contact with ethanol, and then put them into an ultrasonic cleaning machine for ultrasonic cleaning for 15 minutes; take out Pour out the absolute ethanol from the beaker, rinse the enamel slices repeatedly with deionized water; then add deionized water until the liquid surface covers the enamel slices, ultrasonically clean and vibrate for 15 minutes, take out the enamel slices, and dry them.

[0081] ③ Soak the enamel slices in 37% phosphoric acid solution for 60s. Then rins...

Embodiment 2

[0101] During the preparation of the calcium carbonate tie layer, ① take the aspartic acid solution in (2-①) to a volume of 5 mL, and configure it into a 5 mmol / L aspartic acid solution.

[0102] During the preparation of hydroxyapatite crystals, ②add 0.0265g of aspartic acid powder to the Na 2 HPO 4 12H 2 040mL solution was made into a mixed solution of aspartic acid with a content of 5mmol / L.

[0103] Other conditions are as in Example 1, and the crystals obtained on the enamel surface are similar to Example 1. The infrared spectrum of the obtained hydroxyapatite product is shown in Figure 5 A(b).

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Abstract

The invention discloses a method for regenerating a dental prosthetic material and an acidic amino acid-induced demineralized dental enamel outer enamel prism thereof in situ, and belongs to the technical field of in-situ regeneration of dental enamel outer enamel prisms. The preparation method is as follows: firstly, carrying out surface calcium activation onto a dental enamel surface, i.e., grafting calcium ions; then, forming calcium carbonate stable calcium ions step by step; finally, taking calcium carbonate stable calcium ions as foundation forms to synthesize hydroxyapatite crystals. Amino acid participates in the whole process, concentration of the amino acid added in a two-step process is consistent. The hydroxyapatite crystals deposited on the surface of the demineralized dental enamel are orderly and compact in sequence, and uniform in crystal morphology, so that obvious continued growth tendency of an artificial layer can be seen. The preparation method disclosed by the invention lowers protein extracting cost and harsh restrictions on an application environment, and is wide in prospect. The material prepared by the method disclosed by the invention can be applied to cosmetic dental for filling demineralization gaps, also can be used for repairing early-stage enamel demineralization, and can be used as a combined material for bottom pulp capping pit and fissure sealing, and the like.

Description

technical field [0001] The invention belongs to the technical field of in-situ regeneration of tooth enamel in vitro, and specifically relates to a dental repair material and a method for in-situ regeneration of demineralized tooth enamel induced by acidic amino acids. The material prepared by the method can be used for tooth beauty, filling The demineralization gap can also be used for the restoration of early enamel demineralization, and it can also be used as a combined material such as the bottom cover for pulp pit and fissure sealing. Background technique [0002] Dental caries is the primary cause of enamel defect, and it is also the most common oral disease in humans. The incidence of early uncavated enamel caries is significantly higher than that of formed cavities, and when mature enamel is damaged, there are no cells to repair it. Therefore, the early stage of caries is an effective stage for prevention and treatment. The external conditions induce the production o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K6/02A61K6/027A61K6/033A61K6/838
Inventor 赵旭李毅吴晓光
Owner JILIN UNIV
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