The invention relates to an extraction method of
oat bran protein, which comprises the following steps: mixing HBD and HBA according to different
molar ratios, and carrying out magnetic stirring until a clear and transparent liquid is formed; the preparation method comprises the following steps: cleaning oat with deionized water,
drying at 40 DEG C until the
water content is 10%, crushing, sieving with a 60-mesh
sieve, and taking an oversize product of the 60-mesh
sieve as
oat bran; the preparation method comprises the following steps: mixing
oat bran and n-
hexane (g / ml) according to a ratio of 1: 5, magnetically stirring for 3 hours at
room temperature at 600 rpm, centrifuging for 10 minutes at 4000 rpm,
degreasing for 3 times, and naturally volatilizing and airing to obtain degreased oat
bran; mixing the degreased oat
bran and the DES solution in a vortex manner according to a
solid-to-
liquid ratio of 1: 10, stirring for 60 minutes at the temperature of 50 DEG C and at the speed of 600rpm, cooling to
room temperature, and centrifuging for 10 minutes at the speed of 3500rpm; taking the supernatant, extracting the precipitate twice by using the fresh DES in the same extraction process, merging the obtained supernatant, filling the merged supernatant into a
dialysis bag, dialyzing at the temperature of 3,500 D4 DEG C for about 3 days by molecular weight closure, and freeze-
drying the obtained suspension to obtain crude
protein powder.