115 results about "Bovine bone" patented technology

The invention discloses an intelligent fault diagnosis method and system for a cattle bone powder production line, and relates to the related technical field of production fault diagnosis, and the method comprises the steps: collecting equipment operation data and technological parameters of the cattle bone powder production line in real time through a multi-mode sensor, and obtaining a real-time monitoring data set; transmitting the real-time monitoring data set to an edge computing node for preprocessing; inputting the standard fusion data into the cloud digital twin, and dynamically simulating the operation state of the production line to obtain virtual-real mapped production line health state simulation data; analyzing through a hierarchical diagnosis engine to obtain a diagnosis result; and triggering a dynamic response according to a diagnosis result, and performing closed-loop intelligent operation and maintenance of the cattle bone powder production line. The technical problems that in the prior art, fault diagnosis is difficult to conduct on the cattle bone powder production line accurately in real time, the fault diagnosis result lags behind, and the production efficiency and the product quality are affected are solved, and the technical effect of improving the operation stability, the product quality and the production efficiency of the cattle bone powder production line is achieved.

The invention discloses medical soap for sterilizing, relieving itching and removing acne and a manufacturing method thereof, belonging to the field of articles of everyday use. The medical soap is prepared from ingredient A, ingredient B and ingredient C, wherein the ingredient A is prepared from dandelions, folium isatidis, rabdosia rubescens and glabrous sarcandra herb; the ingredient B is prepared from radix angelicae, rhizoma typhonii, white poria, Chinese angelia root tai, radix pseudostellariae, raw milkvetch root, Chinese honeylocust fruit, red paeony root, prepared kusnezoff monkshood root, radices lithospermi, cortex dictamni, thunberg fritillary bulb, fiveleaf akebia fruit, white muscardine silkworms and solanum lyratum; the ingredient C is prepared from soap base, honey and bovine bone marrow oil. The medical soap disclosed by the invention is used for external washing or smearing after being wet by adding water in the morning and evening every day; after a person with smelly feet washes the feet, the medical soap is smeared in gaps between toes; and the medical soap is not suitable for use when the skin is damaged. The medical soap prepared by the invention is a black brown solid body, and has soap medicine odor; the medical soap can be used for nourishing the skin, is antiallergic, beautifies the features, removes acne, relieves itching, removes freckles, clearing heat and deterring sweat; the medical soap can beautify the features, whiten the skin, and remove the acne and the freckles when used for washing the face, and can deter the sweat, remove the smelly odor and treat dermatophytosis when used for washing the feet; and the medical soap has a control effect on the itching toxin which appears after mosquito bite, and has a moisturizing effect for the person having the dry skin.

The invention relates to the technical field of collagen preparation, in particular to a collagen product as well as a preparation method and application thereof. The collagen product prepared by the invention overcomes the problems of poor dissolution effect in cold water, poor fat solubility and poor emulsifying property. The preparation method comprises the following steps: crushing bovine bones, and performing collagen extraction, enzymolysis treatment, glycosylation treatment, fatty acid amidation treatment, polyphenol grafting modification and colloid compounding to obtain the collagen product. During glycosylation treatment, the cold water dissolving effect is improved by adjusting the variety of reducing sugar, the reaction temperature and time and grafting bone peptide molecules to sugar chains, and a flavor substance is generated by the Maillard reaction in the glycosylation treatment, so that the effect of covering the fishy smell of cattle bones is achieved; relevant parameters are adjusted through fatty acid amidation treatment, the bone peptide is endowed with liposolubility and emulsibility, and the solubility and emulsibility of the collagen product are synergistically improved through colloid compounding, optimization of the use amount of various colloids and control of reaction conditions.

The invention discloses a pressure-controlled bovine bone collagen active peptide, and belongs to the field of food. A potential ACE inhibitory peptide is rapidly screened through a bioinformatics method, the ACE inhibitory activity of the bovine bone collagen peptide is verified in combination with an in-vitro experiment, IC50 values of the screened bovine bone collagen active peptides LGALPGF, VFPGLL and AAAALGPW to ACE are 32.07 mu M, 40.41 mu M and 77.68 mu M respectively, and the peptide with the sequence is proved to have a good antihypertensive function.

The invention provides a nutrition enhancer beneficial to bone health of middle-aged and elderly people, a preparation method of the nutrition enhancer and application of the nutrition enhancer in preparation of medial care food, and belongs to the field of nutritious food. The nutrient supplement comprises a plant fermentation product, osteopontin peptide, collagen peptide, N-acetylglucosamine and calcium, vitamins and mineral substances can be added, and the plant fermentation product is obtained by performing anaerobic fermentation on rhizoma drynariae and chestnuts through a complex microbial inoculant composed of lactobacillus reuteri and bifidobacterium longum subsp. Longum; the collagen peptide is prepared by performing enzymolysis on bovine bones through alkaline protease and flavourzyme; the hydrogel is prepared by adopting a sodium alginate-calcium chloride-carboxymethyl chitosan micro-capsule embedding process. An in-vitro experiment shows that the nutrition enhancer can remarkably promote osteoblast proliferation, differentiation and mineralization; animal experiments show that the bone mineral density of osteoporosis model mice can be effectively improved. The nutrition enhancer disclosed by the invention is suitable for preparing care food and bone health products, and has a remarkable effect of improving bone health of middle-aged and elderly people.

The invention relates to the technical field of biological processing, in particular to a production process and application of bovine bone collagen peptide for preventing osteoporosis and delaying cell senescence, and the production process comprises the following steps: placing yak bone into a bone placing cylinder, and plugging a rubber plug; a first motor and a second motor are started to drive a shifting block and a pushing block to quickly move from one end of a bone placing cylinder to the other end of the bone placing cylinder in a reciprocating manner, so that a plurality of bone striking groups inserted outside the bone placing cylinder are triggered to be compressed, rebounded and released, yak bones in the bone placing cylinder are struck, and concave holes and cracks are formed in the bone surface; and then the servo motor is started to drive the bone unloading belt to unload the yak bones, and the yak bones fall into the bone crushing machine body to be crushed. The spring steel conical head is driven by the cambered surface and planar force storage group to pre-split yak bones, directional impact on the surfaces of the yak bones is completed, continuous compression and release are achieved by linkage of the two-way lead screw and the shifting block and the pushing block, full-circumferential dead-corner-free impact on the yak bones is completed, the effect of increasing the crack coverage rate is achieved, the extrusion force of a subsequent bone crusher is reduced, and the production efficiency is improved. The service life of the double-tooth roller is prolonged.

The invention relates to the field of medicines, and discloses slow-release composite powder for removing nitrite and a preparation method thereof, and the slow-release composite powder comprises the following raw materials: bovine bone collagen peptide, sorbitol, L-sodium ascorbate, L-alanine composite powder, vitamin E, earthworm protein, natto powder, cranberry powder and magnesium stearate. Wherein the L-alanine composite powder is prepared from L-alanine, y-aminobutyric acid and L-glutamic acid; according to the present invention, the L-alanine composite powder and the active substance can chemically react with the nitrite in the body so as to convert the nitrite into the safe substance to be discharged out of the body, such that the absorption and the adsorption of the nitrite can be easily reduced, and the formation of the nitrite in the digestive tract can be reduced so as to reduce the concentration of the nitrite in the body; and nitrite is directly prevented from being accumulated to the level of potential health hazards.

The invention discloses a multifunctional bone peptide compound and application thereof in prevention and treatment of osteoporotic osteoarthritis, and relates to the technical field of bone peptides. The multifunctional bone peptide compound is prepared from the following raw materials: giant salamander bone meal, bovine bone articular cartilage powder, bovine bone meal, sturgeon bone meal, oyster bone meal, deer-horn glue powder, calcium chloride and fructo-oligosaccharide. According to the multifunctional bone peptide compound disclosed by the invention, the composite aggregate is subjected to enzymolysis step by step by using the compound enzyme, the bone peptide and calcium ions are compounded through a chelation reaction, the absorbability of calcium is improved, one part of the bone peptide in the composite bone peptide has a function of promoting osteoblast activity, and the other part of the bone peptide possibly has a function of inhibiting overactivity of osteoclasts; part of the bone peptide has an anti-inflammatory effect, can regulate bone metabolism, repair articular cartilage and relieve inflammatory response, and can improve the effect of the bone peptide product in the aspects of preventing and treating osteoporosis, osteoarthritis and other diseases.

The invention belongs to the technical field of hotpot condiments, and particularly relates to a bovine bone marrow oil mellow and hot hotpot condiment and a preparation method thereof. Comprising the following raw materials in parts by weight: 205 to 298 parts of edible beef tallow, 9.5 to 14 parts of edible bovine bone marrow oil, 15 to 25 parts of dried chili, 18 to 26 parts of chafing dish bean halves, 4 to 6 parts of fermented soya beans, 13 to 20 parts of monosodium glutamate, 10 to 16 parts of edible salt, 7 to 12 parts of fresh ginger, 9 to 14 parts of garlic, 7 to 11 parts of shallots, 10 to 16 parts of spices, 4.5 to 7 parts of yeast extract, 3 to 4.5 parts of white granulated sugar, 0.5 to 0.97 part of disodium 5 '-ribonucleotide, 15.8 to 23.8 parts of edible flavors and fragrances and 0.5 to 0.9 part of capsanthin. The edible bovine bone marrow oil in the mellow and hot hotpot condiment product containing the bovine bone marrow oil has rich and deep animal fat fragrance, the fragrance is mellower than beef tallow, the condiment is good in mellowness, and the flavor and taste are improved.

The present application relates to a kind of technology for inducing marine sessile organism attachment, especially a kind of fiber reinforced ecological concrete and preparation method, belong to the cross field of marine sessile organism and marine concrete.Its material components include: cementitious material, broken stone, sand, water, dark pigment, biological calcium powder, calcium carbonate powder, trace element, chopped fiber and superplasticizer.The purpose of the present application is to induce sessile organism attachment, mainly oyster, in the tidal zone reinforced concrete corrosion prevention, considering the attachment of barnacle.By controlling to use dilute acid modification and composite grinding technology, the inducing capacity of bovine bone powder is fully exerted, the bovine bone powder content is greatly reduced, and corrosion prevention treatment and modification are carried out, a composite inducer mainly composed of bovine bone powder is realized, the content is small, and the concrete strength and permeability are almost not affected.

The invention discloses a preparation method of collagen micromolecule active peptide and bioactive peptide for inhibiting cancer cell proliferation and improving intracellular environment, and belongs to the technical field of bioactive peptide preparation and biological functional raw materials. According to the method, bone collagen peptide, cartilage peptide (hydrolyzed II-type collagen peptide), collagen peptide (German Jilida), non-denatured II-type collagen peptide and yak bone marrow peptide are taken as core raw materials, and optional ingredients such as bovine bone marrow peptide powder, shark cartilage peptide and Belgian imported inulin can be compounded as required; through a step-by-step preparation process comprising the steps of graded purification pretreatment of raw materials, precise proportioning and mixing, low-temperature activation and synergism and stable micro-capsule embedding, structure protection and efficacy synergy of each collagen small-molecule active peptide and bioactive components are realized; the prepared bioactive peptide product can significantly inhibit cancer cell proliferation, regulate intracellular oxidation-reduction balance and improve intracellular microenvironment, the preparation process is mild and free of high-temperature damage, the raw material biological activity retention rate is high, the raw material source is controllable, the large-scale production feasibility is high, and the bioactive peptide product can be widely applied to preparation in the fields of functional food, bioactive raw materials and the like.

1. The name of the design product: packaging box (solid beverage of bovine bone marrow peptide). 2. The use of the design product: the design product is used for beverage packaging box. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view 1. 5. The top view has been expressed in the top view enlarged view, and the top view is omitted; the bottom view has been expressed in the bottom view enlarged view, and the bottom view is omitted.

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for improving a cognitive function as well as a preparation method, a product and application of the composition. The composition is prepared by taking soybean meal, whey protein peptide powder and bovine bone collagen peptide powder in a mass ratio of 1: 3: 2 as peptide source raw materials, matching the peptide source raw materials with AMY001, lactobacillus plantarum LP-90 and clostridium butyricum (3: 1: 1) compound strains, and carrying out enzyme production self-hydrolysis by bacillus amyloliquefaciens, peptide-bacterium anaerobic synergistic fermentation and sodium alginate-HPMC-CPP composite carrier embedding. According to the present invention, the hippocampus Abeta1-42 protein can be cleared, the BDNF content can be increased, the IL-6 expression inhibition multi-mechanism synergistic effect can be achieved, the memory improvement rate and the Abeta1-42 clearance rate are high, and the cognitive impairment can be significantly repaired. The composition is high in stability and bioavailability, controllable in preparation process and suitable for development of food and health care products for improving the cognitive function and medicines for preventing or assisting in improving diseases related to cognitive impairment.

The invention belongs to the technical field of biological medicine, and particularly relates to a preparation method and application of bovine bone marrow peptide. The bovine bone marrow peptide is prepared by performing enzymolysis on bovine bone marrow protein. The bovine bone marrow peptide can effectively enhance the activity of immune cells and / or promote the proliferation of the immune cells. The polypeptide can improve the immunity of the organism, has the advantages of being simple to prepare, easy to absorb and the like, and is easy to popularize and apply.

The utility model discloses a kind of cattle bone removing device with pneumatic clamping mechanism, including processing table, processing table, the side of top end is provided with cutting mechanism, and the other side of processing table top end is provided with clamping mechanism, the opening of the surface of processing table bottom end is fixed with export box, and the both sides of processing table bottom end are fixedly provided with support frame, clamping mechanism includes support frame, the inner wall of support frame is rotatably connected with two-way screw rod, the both sides of two-way screw rod outer wall are all screw-connected with square moving block, the bottom of each square moving block is fixedly provided with movable column. The utility model is cooperated by cutting mechanism and clamping mechanism, in clamping mechanism, cylinder drives push block, and then drive clamping arm through first connecting rod, can be respectively clamped and fixed beef from two sides, simultaneously, two-way screw rod drives two housings to be close or away, and then guarantee to tighten beef, ensure the bone operation of beef, avoid deviation in bone removing process, improve bone removing efficiency.

The bovine bone fusion cage driver convenient to adjust comprises a handle, a clamping device is arranged, a fusion cage is arranged on the inner side of an elastic clamping piece, a rotating rod is rotated through a rotary knob, the rotating rod drives a rotating shaft and a driving gear to rotate, the driving gear drives a driven gear to rotate through the action of tooth meshing, and the driven gear drives the driven gear to rotate. Due to the effect of threaded connection, the driving gear rotates and drives the threaded sleeve to move forwards at the same time, the threaded sleeve exerts external force on the elastic clamping pieces, the elastic clamping pieces are made to get close to the middle, closing-up is achieved, the effect of clamping the fusion cage is achieved, then the reverse rotating knob is loosened, the threaded sleeve moves backwards to the initial position through the effect of threaded connection, and the fusion cage is clamped. At the moment, the external force acting on the elastic clamping piece disappears, the elastic clamping piece also restores to the initial open state, the device can be moved out, implantation is completed, the fusion cage is not subjected to any external force in the whole implanting and moving-out process, and the risk of loosening cannot occur.

The invention relates to a bovine bone collagen concentration prediction method and device based on spectral data, and belongs to the technical field of artificial intelligence. The method comprises the following steps: collecting near infrared spectrum data of a bovine bone sample, and constructing a training data set; processing the near infrared spectrum data of the bovine bone sample to obtain smooth spectrum data; carrying out local differential enhancement on a characteristic peak edge on the spectral data by adopting a dual-channel characteristic enhancement mechanism, and carrying out global transformation to obtain a global transformation characteristic value; constructing a bovine bone collagen concentration prediction model which comprises a cross-layer spectrum section attention residual module, a spectrum-concentration correlation gating module, a bovine bone collagen concentration prediction module and a loss function; inputting the global transformation characteristic value into a bovine bone collagen concentration prediction model, and training and optimizing the model; and processing to-be-detected data, and inputting the processed to-be-detected data into the trained model to obtain a prediction result of the concentration of the bovine bone collagen. According to the method, the accuracy of predicting the concentration of the bovine bone collagen can be improved.

The invention belongs to the technical field of food preparation, and particularly relates to a biological information technology guidance-based compound bovine bone collagen peptide beverage, which is prepared from the following raw materials: bovine bone collagen peptide powder, red date polysaccharide, astragalus polysaccharide, calcium carbonate, chondroitin sulfate and sodium hyaluronate. When the compound bovine bone collagen peptide beverage is drunk, bovine bone contains a large amount of collagen, is rich in type I collagen and is richer in amino acid composition, and a large number of research results show that protein is not completely hydrolyzed into amino acid after being ingested, most of the protein is absorbed in a peptide form, and the protein is absorbed in a peptide form. Therefore, the selected bovine bone collagen peptide powder can be better absorbed by the human body. The red dates have the effects of maintaining beauty, keeping young and resisting aging. In addition, astragalus membranaceus and other traditional Chinese medicine extracts are supplemented in the compound bovine bone collagen peptide beverage, so that the functional characteristics of the beverage are enhanced, and the original bad flavor of animal peptide is improved.

The invention relates to the technical field of small molecular weight salty peptides, and particularly discloses a dipeptide DP with a salty taste increasing function, a preparation method and a system, the dipeptide is DP, and the amino acid sequence of the dipeptide is Asp-Pro; the molecular formula of the dipeptide is C9H14N2O5; mixing bovine bone powder with water; adding trypsin, compound protease and flavourzyme to carry out enzymolysis reaction; enzyme deactivation; centrifuging the solution through a centrifugal assembly, and taking supernate by adopting a suction assembly; enabling the supernate to sequentially pass through ultrafiltration membranes of 3kDa and 1kDa to obtain a molecular weight gt; 3 kDa, 1 kDa to 3 kDa, and lt; a component of 1 kDa; the molecular weight 1t is taken; carrying out gel permeation chromatography separation and purification on the components of 1 kDa and 1 kDa to obtain a peak group; performing amino acid sequence identification on components corresponding to the peak group, and performing preliminary screening through C-terminal amino acid composition of peptide; and carrying out molecular docking on the primarily screened peptide and a salty receptor, and obtaining the dipeptide DP according to binding energy. The invention has the characteristics of no reduction of sensory quality, easy and rapid absorption and simple and convenient preparation steps.

The invention relates to the technical field of collagen peptide production, in particular to bovine bone collagen peptide production equipment which comprises a shell and a partition plate, a stirring shaft is rotatably connected to the interior of the shell, stirring blades are fixed to the surface of the rotating shaft, the partition plate is arranged in the middle of the shell, and the stirring blades are fixed to the surface of the stirring shaft. The interior of the shell is divided into an upper cavity and a lower cavity by the partition plate, and a communicating groove for communicating the upper cavity with the lower cavity is formed in the partition plate; according to the cattle bone powder mixing device disclosed by the invention, the separation plate is arranged in the shell to separate a production space, and cattle bone powder with high density can be quickly settled due to the gravity effect in a mixing process, so that the cattle bone powder with high density is hydrolyzed in the lower space; and the cattle bone powder with low density in the upper cavity continues to react in the original environment, so that the problems of excessive partial hydrolysis and insufficient partial hydrolysis caused by mixing are avoided, and the quality of a finished product of collagen peptide is favorably stabilized.

The invention discloses a composition containing bovine bone collagen as well as a preparation method and application thereof, and belongs to the technical field of collagen application. The composition containing the bovine bone collagen is obtained by chemical crosslinking of bovine bone collagen magnesium chelate, sodium hyaluronate, chondroitin sulfate and bovine bone collagen through EDC / NHS. The composition containing bovine bone collagen is prepared from the following components in parts by weight: 5 to 10 parts of bovine bone collagen magnesium chelate, 2 to 5 parts of sodium hyaluronate, 0.5 to 2 parts of chondroitin sulfate and 20 to 30 parts of bovine bone collagen. The bovine bone collagen magnesium chelate, the sodium hyaluronate, the chondroitin sulfate and the bovine bone collagen in the composition containing the bovine bone collagen prepared by the invention have the characteristics of good biocompatibility, capability of promoting cell proliferation and differentiation into cartilage cells, and suitability for cartilage repair.

The invention relates to a finishing agent for improving washing resistance of down and a preparation method thereof, and relates to the technical field of finishing agent preparation, the finishing agent comprises, by weight, 40-50 parts of polyurethane resin, 20-30 parts of epoxy resin, 1-2 parts of a cross-linking agent, 3-6 parts of bovine bone glue, 3-6 parts of konjac glucomannan and 10-15 parts of deionized water; the bovine bone glue is obtained by mixing common bovine bone glue and chitosan; the konjac glucomannan is obtained by catalyzing common konjac glucomannan through pectinase. According to the finishing agent, by adding the bovine bone glue and the konjac glucomannan, the washing resistance of down feather can be remarkably improved, the adhesive force of the finishing agent on the down feather is improved, the stability and durability of the down feather in the washing process are enhanced, meanwhile, the bovine bone glue and the konjac glucomannan are both natural products, and environmental pollution in the production process of the finishing agent can be reduced; the water resistance and the stain resistance of the down feather are improved.

The application provides yak cartilage peptide with a function of repairing cartilage injury, and a preparation method and application thereof, and belongs to the technical field of active peptides. The application selects crescent bones of yaks as raw materials, adopts papain and trypsin for complex enzymolysis, and combines with ultrasonic-assisted enzymolysis technology, so that the enzymolysis efficiency and peptide yield are greatly improved. The yak cartilage peptide prepared by the application is proved to have good antioxidant activity and the effect of promoting cartilage cell proliferation through in-vitro cell experiments, and the effect is significantly better than that of traditional enzymolysis methods and commercial pig bone peptide, fetal bovine bone peptide and other products. Therefore, the yak cartilage peptide prepared by the application can be used for repairing cartilage injury, treating cartilage degeneration and improving symptoms related to osteoarthritis.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine preparation for strengthening bones and tendons and improving osteoporosis and a preparation method thereof. The traditional Chinese medicine preparation is prepared from the following raw material medicines in parts by weight: 25 to 30 parts of bovine bone, 15 to 20 parts of penis cervi, 10 to 15 parts of fructus mali, 8 to 12 parts of fructus corni, 8 to 10 parts of radix angelicae sinensis, 8 to 12 parts of semen sojae germinatum, 8 to 10 parts of rhizoma dioscoreae, 5 to 10 parts of rhizoma polygonati and 5 to 10 parts of liquorice root. In the formula, bovine bone and penis et testis cervi are taken as monarch drugs, malaytea scallop fruit, dogwood and angelica sinensis are taken as ministerial drugs, semen sojae germinatum, Chinese yam and rhizoma polygonati are taken as adjuvant drugs, and liquorice is taken as a conductant drug. All the components of the traditional Chinese medicine preparation have a synergistic effect, and the purpose of treating osteoporosis is achieved by adjusting the levels of ALP, OC and TRACP-5b, improving bone metabolism, increasing bone mineral density and inhibiting bone resorption.

The invention provides a high-purity bovine bone collagen peptide gel filtration chromatography purification method, and belongs to the technical field of bioactive peptide purification. The method comprises the following five core steps: pretreatment, gel column preparation, dynamic equilibrium, sample loading and elution, and post-treatment: firstly, carrying out ultrafiltration pretreatment on a bovine bone collagen peptide crude extract, then filling a chromatographic column with a specific diameter-height ratio by using cross-linked sephadex, carrying out dynamic equilibrium by using an accurately adapted equilibrium liquid after column efficiency verification, and finally, carrying out dynamic equilibrium by using a chromatographic column with a specific diameter-height ratio. The sample loading amount is dynamically matched through a formula, a gradient isothermal mode is adopted in the elution process, and finally a high-purity product is obtained through freeze drying. Through multi-step parameter collaborative optimization and precise regulation and control of a self-created formula, the problems of low purification efficiency, poor product purity and wide molecular weight distribution in the prior art are solved, the purity of the obtained bovine bone collagen peptide is greater than or equal to 98%, the proportion of peptide fragments with molecular weight distribution of 500-3000Da and 1000-2000Da is greater than or equal to 70%, the heavy metal content and the colony count both meet high-standard requirements, the yield is stabilized at 52% or above, and the method is suitable for industrial production. Good industrial application prospects are realized.

The invention belongs to the technical field of coking wastewater treatment, and particularly relates to a method for preparing a boron-doped bone black catalyst and pretreating coking wastewater, boron-doped bone black is bone black doped with boron, and the method comprises the following steps: 1, preparing bone meal slurry: performing high-temperature treatment on animal bones, and crushing and grinding to obtain bone meal; bone meal and deionized water are mixed, stirred and subjected to ultrasonic treatment; 2, preparing bone black precursor powder: adding a boron-containing compound into the bone powder slurry, stirring at a constant temperature, evaporating to dryness, and grinding; 3) bone pre-carbonization: performing high-temperature pre-reaction on the bone black precursor powder; and grinding, calcining in an inert atmosphere, grinding, pickling, alcohol washing and drying to obtain the boron-doped bone black. The boron-doped bovine bone charcoal has the advantages that the micro-nano structure of the bone charcoal is remarkably improved by boron doping, the formation of BCO2 and BC2O in the bone charcoal is promoted by boron doping, and the mesopore and macropore ratio of the bovine bone charcoal is increased, so that the boron-doped bovine bone charcoal is an irregular particle with a rough surface and a hierarchical pore structure.

The invention discloses brevibacillus laterosporus SCUT-BR-2 capable of producing collagenase and a method for applying the brevibacillus laterosporus SCUT-BR-2 to extraction of animal collagen / collagen peptide. The method specifically comprises the following steps: screening a bacterial strain capable of producing collagenase from a meat product, carrying out morphological observation, physiological and biochemical tests and determination, extracting collagen in animal skin by adopting a collagenase crude extract of the bacterial strain, and detecting the relative molecular weight and the like of the collagen; meanwhile, bovine bone collagen is decomposed by adopting the crude extract, and the bovine bone collagen peptide which is good in oxidation resistance and has anti-wrinkle, firming, soothing and moisturizing effects is obtained.

The invention relates to the technical field of small molecular weight salty peptides, and particularly discloses a salty and fresh peptide RRRRR with a salty and fresh increasing function and a screening method, the salty and fresh peptide is RRRRR, and the amino acid sequence of the salty and fresh peptide is Arg-Arg-Arg-Arg-Arg; the molecular formula of the salty fresh peptide is C30H62N20O6; mixing bovine bone powder with water; adding trypsin, compound protease and flavourzyme to carry out enzymolysis reaction; enzyme deactivation; centrifuging the solution through a centrifugal assembly, and taking supernate by adopting a suction assembly; enabling the supernate to sequentially pass through ultrafiltration membranes of 3kDa and 1kDa to obtain a molecular weight gt; 3 kDa, 1 kDa to 3 kDa, and lt; a component of 1 kDa; the molecular weight 1t is taken; carrying out gel permeation chromatography separation and purification on the components of 1 kDa and 1 kDa to obtain a peak group; performing amino acid sequence identification on components corresponding to the peak group, and performing preliminary screening through C-terminal amino acid composition of peptide; and carrying out molecular docking on the primarily screened peptide and a salty receptor, and obtaining the salty and fresh peptide RRRRR according to binding energy. The invention has the characteristics of no reduction of sensory quality, easy and rapid absorption and simple and convenient preparation steps.

This invention belongs to the field of genetic engineering technology and discloses a high-efficiency expression method for recombinant collagenase and its application in the preparation of bovine bone collagen peptides. The collagenase gene derived from Bacillus sp. HUB-I-004 strain (accession number J9CTX3) was optimized using E. coli-preferred codons. A recombinant expression vector was constructed and transformed into E. coli BL21(DE3). After optimization of induction conditions and Ni-NTA affinity purification, a highly active recombinant collagenase with a specific activity of 231.05 U / mg was obtained. Bovine bone collagen was extracted using a high-temperature, high-pressure method. Bovine bone collagen peptides were prepared under optimal conditions of a substrate concentration of 10 mg / mL, an enzyme addition of 20%, and enzymatic hydrolysis at 37℃ for 8 h, significantly improving peptide yield. The obtained product is mainly composed of small molecule peptides and exhibits significant osteoblast proliferation-promoting activity, making it widely applicable in the preparation of health foods and pharmaceuticals.