Preparation method of bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis

Abstract

The invention relates to the technical field of biology, in particular to a preparation method of a bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis. According to the preparation method, a combined enzymolysis process of acid protease and neutral protease is adopted, and the generation rate of a specific functional peptide fragment is increased through intelligent enzyme cutting site design; the biological activity of the product is enhanced, so that the treatment effect of the product on osteoporosis and arthritis is improved; key functional components such as active peptide fragments, turmeric and black pepper extracts are protected through a nano embedding technology, so that the key functional components are prevented from being damaged in the production process, and absorption and utilization of the components in vivo are promoted; the stability and bioavailability of the functional components are improved, the absorption enhancing mechanism is beneficial to more effectively promoting bone calcium deposition and relieving joint inflammation, the high-pressure homogenizer technology is adopted, it is guaranteed that the functional components are evenly distributed in the product, and the problem that the effect is unstable due to uneven component distribution is avoided.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to a method for preparing a bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis. Background Art

[0002] Bovine bone protein peptide is a bioactive substance extracted from bovine bones and is widely used in the fields of bone health and joint protection. At the same time, bovine bone protein peptide, as a natural bioactive ingredient, has received widespread attention in recent years due to its potential role in promoting bone health and alleviating arthritis.

[0003] As the population ages, the incidence of osteoporosis and arthritis increases year by year, especially among the elderly. These problems have become major health challenges that seriously affect the quality of life. Bovine bone protein peptide has gradually become a research hotspot in the field of health food and medicine because of its functions of promoting bone growth, enhancing bone density, and improving joint health.

[0004] However, the traditional bovine bone protein peptide preparation method has deficiencies in the extraction efficiency of functional ingredients, protection of biological activity, product stability and uniformity, resulting in certain limitations in its actual effect on improving osteoporosis and relieving arthritis: the enzymatic hydrolysis process for the preparation of the current bovine bone protein peptide composition has problems of low enzymatic hydrolysis efficiency and polypeptide uniformity, and the enzymatic hydrolysis process uses a single enzyme system, resulting in uneven protein fragmentation, making it difficult to efficiently generate and enrich specific functional peptide segments, resulting in limited efficacy of the final product in improving osteoporosis and relieving arthritis; the functional ingredients in the bovine bone protein peptide that have anti-inflammatory and bone health-promoting properties are easily lost or damaged during the production process, reducing the biological activity and therapeutic effect of the product; and the uniformity and stability of the product ingredients are poor, and the functional ingredients in the product are unevenly distributed, affecting its overall therapeutic effect on osteoporosis and arthritis; therefore, there is an urgent need for a preparation method for a bovine bone protein peptide composition that can improve osteoporosis and relieve arthritis to solve such problems. Summary of the invention

[0005] To this end, the present invention provides a method for preparing a bovine bone protein peptide composition that can improve osteoporosis and relieve arthritis, so as to overcome the problems in the prior art of insufficient generation and utilization of functional peptide segments, insufficient loss of functional ingredients and insufficient protection of activity, and poor uniformity and stability of product ingredients.

[0006] To achieve the above object, on the one hand, the present invention provides a bovine bone protein peptide composition that can improve osteoporosis and relieve arthritis. The bovine bone protein peptide composition is prepared from the following raw materials in parts by weight: 20-45 parts of bovine bone protein peptide, 2-4 parts of soy protein isolate, 5-12 parts of oligofructose, 25-35 parts of whey protein, 15-20 parts of maltodextrin, 5-15 parts of kudzu root extract, 3-5 parts of bonito elastin peptide, 2-3 parts of enzymatic bone powder, and nano-precious 1-10 parts of pearl powder, 0.8-2.8 parts of wolfberry peptide powder, 0.7-0.9 parts of xanthan gum, 0.5-1.5 parts of turmeric, 0.5-0.75 parts of elderberry juice powder, 0.4-0.45 parts of vitamins A, D, C, 0.05-1 parts of polygonatum peptide powder, 0.05-0.2 parts of casein phosphopeptide, 0.02-0.05 parts of flavor, 0.008-0.02 parts of sucralose, and 0.005-0.18 parts of black pepper extract.

[0007] Furthermore, the bovine bone protein peptide composition is prepared from the following raw materials in parts by weight: 25 parts of bovine bone protein peptide, 2 parts of soy protein isolate, 5.5 parts of fructooligosaccharide, 30 parts of whey protein, 18 parts of maltodextrin, 6 parts of kudzu root extract, 4 parts of bonito elastin peptide, 2.5 parts of enzymatic bone powder, 6.32 parts of nano pearl powder, 1 part of wolfberry peptide powder, 0.8 parts of xanthan gum, 1 part of turmeric, 0.8 parts of elderberry juice powder, 0.46 parts of vitamins A, D and C, 0.09 parts of polyanthocyanidin powder, 0.2 parts of casein phosphopeptide, 0.04 parts of flavor, 0.018 parts of sucralose and 0.16 parts of black pepper extract.

[0008] On the other hand, the present invention also provides a method for preparing a bovine bone protein peptide composition that can improve osteoporosis and relieve arthritis, comprising:

[0009] Step S1, degreasing cattle bones, crushing cattle bones into pieces of 1-5 cm in size and adding them into a degreasing tank; adding purified water into the degreasing tank at a ratio of 1:1, and boiling for 4 hours; lowering the temperature of the degreasing tank to 75°C, and the vacuum degree to -0.045MPa to -0.12MPa, and separating the upper layer of fat; boiling at normal pressure, and then continuing to add purified water into the degreasing tank at a ratio of 1:1, heating to 100°C, and boiling for 4 hours; and separating the upper layer of fat after standing;

[0010] Step S2, acid treatment, the defatted cattle bones are placed in a hydrochloric acid solution with a mass fraction of 1.15% and immersed for 4 hours;

[0011] Step S3, bone mud preparation, cattle bones are crushed and ground into bone mud, added into a 2.5% by mass vitamin C aqueous solution, and soaked for 8h-10h to obtain an enzymatic substrate; the usage ratio of cattle bones to vitamin C aqueous solution is 1kg:3.2L;

[0012] Step S4, enzymatic hydrolysis, adding acidic protease for enzymatic hydrolysis; adjusting the pH to 7, adding neutral protease for enzymatic hydrolysis; after the enzymatic hydrolysis is completed, raising the temperature to terminate the reaction;

[0013] Step S5, filtering and concentrating, filtering to obtain a hydrolyzate;

[0014] Step S6, mixing with a high pressure homogenizer, mixing the concentrate and all other dry powder ingredients; using a high pressure homogenizer to process the mixture to form a uniform mixture;

[0015] Step S7, embedding and drying, embedding and drying the homogenized mixed solution to prepare a granular bovine bone protein peptide composition.

[0016] Furthermore, in step S2, the usage ratio of the cattle bone to the hydrochloric acid solution is 1 kg:3.2 L.

[0017] Furthermore, in step S4, acidic protease is added in a mass ratio of 1%, and after enzymatic hydrolysis for 3-4 hours to adjust the pH to 7, neutral protease is added in a mass ratio of 1% for enzymatic hydrolysis for 2-3 hours.

[0018] Furthermore, in step S5, the hydrolyzate is concentrated using a scraper concentrator to obtain a concentrated extract having a mass fraction of 35%.

[0019] Furthermore, in step S6, other dry powder ingredients include: maltodextrin, vitamins, and extracts.

[0020] The present invention also discloses the use of the above-mentioned bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis in the preparation of health products. The health products include bovine bone protein peptide beverage powder, bovine bone protein peptide capsules and bovine bone protein peptide solid beverages. The health products are used to promote bone growth, promote calcium absorption, enhance bone density and improve osteoporosis.

[0021] The invention also discloses a health product containing the bovine bone protein peptide composition which can improve osteoporosis and relieve arthritis. The health product includes the bovine bone protein peptide composition.

[0022] Furthermore, the content of the bovine bone protein peptide composition in the health product is 100%.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention adopts a combined enzymatic hydrolysis process of acidic protease and neutral protease, and through intelligent enzyme cleavage site design, improves the generation rate of specific functional peptides (peptides that promote bone health and have anti-inflammatory effects); enhances the biological activity of the product, especially in promoting bone density increase and relieving joint inflammation, thereby improving the product's therapeutic effect on osteoporosis and arthritis.

[0025] The present invention protects key functional ingredients such as active peptides, turmeric and black pepper extracts through nano-encapsulation technology to prevent them from being destroyed during the production process, thereby promoting the absorption and utilization of these ingredients in the body; the stability and bioavailability of the functional ingredients are improved, and the mechanism of enhanced absorption helps to more effectively promote bone calcium deposition and relieve joint inflammation.

[0026] The present invention adopts high-pressure homogenizer technology to ensure the uniform distribution of functional ingredients in the product, avoiding the problem of unstable efficacy caused by uneven distribution of ingredients; the homogenization technology significantly improves the uniformity and stability of the product, ensuring that each dose of the product has a consistent therapeutic effect in improving osteoporosis and relieving arthritis. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The present invention is a schematic diagram of the preparation method of the bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis. DETAILED DESCRIPTION

[0028] In order to enable those skilled in the art to better understand the scheme of the present invention, the technical scheme in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0029] It should be noted that the terms "first", "second", etc. in the specification and claims of the present invention and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the data used in this way can be interchanged where appropriate, so that the embodiments of the present invention described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions, for example, a process, method, system, product or device that includes a series of steps or units is not necessarily limited to those steps or units that are clearly listed, but may include other steps or units that are not clearly listed or inherent to these processes, methods, products or devices.

[0030] The present invention is further described in detail below in conjunction with the accompanying drawings:

[0031] Example 1

[0032] A bovine bone protein peptide beverage powder, the raw material ratio includes: 30 parts of bovine bone protein peptide, 6 parts of oligofructose, 25 parts of whey protein, 20 parts of maltodextrin, 5 parts of kudzu root extract, 3 parts of bonito elastin peptide, 3 parts of enzymatic bone powder, 7 parts of nano pearl powder, 0.5 parts of wolfberry peptide powder, and 0.5 parts of xanthan gum;

[0033] The preparation steps of the bovine bone protein peptide beverage powder include:

[0034] Pretreatment and acid treatment of cattle bones: follow steps 1 and 2 in the basic preparation method; i.e.

[0035] Degreasing of cattle bones: Crush the cattle bones to 2 cm in size and add them to the degreasing tank; Add purified water to the degreasing tank at a ratio of 1:1 and boil for 4 hours; Lower the temperature of the degreasing tank to 75°C, and the vacuum degree to -0.089MPa to separate the upper layer of fat; Steam at normal pressure, and then continue to add purified water to the degreasing tank at a ratio of 1:1, raise the temperature to 100°C, and steam for 4 hours; After standing, separate the upper layer of fat;

[0036] Acid treatment: After defatting, the cattle bones were immersed in a 1.15% hydrochloric acid solution for 4 h;

[0037] Enzymatic hydrolysis process: Use acidic protease and neutral protease for double enzymatic hydrolysis to ensure efficient protein decomposition.

[0038] Mixing and Homogenization: The enzymatically digested liquid is mixed with all dry powder ingredients and processed using a high pressure homogenizer to form a uniform mixture.

[0039] Drying and packaging: After spray drying, fine powder is obtained and packaged in small single-use packages for consumers to brew and drink directly.

[0040] Example 2

[0041] A bovine bone protein peptide capsule, comprising: 40 parts of bovine bone protein peptide, 0.2 parts of polyanthocyanin powder, 0.3 parts of casein phosphopeptide, 0.2 parts of black pepper extract, 1 part of vitamin mixture, and 58.3 parts of filler (microcrystalline cellulose);

[0042] The preparation steps of bovine bone protein peptide capsules include:

[0043] Enzymatic hydrolysis and concentration: Follow steps 4 and 5 in the basic preparation method to obtain high-concentration protein peptides, namely:

[0044] Enzymatic hydrolysis, adding acidic protease at a mass ratio of 1% in sequence, adjusting the pH to 7 after enzymatic hydrolysis for 4 hours, and then adding neutral protease at a mass ratio of 1% for enzymatic hydrolysis for 3 hours; after the enzymatic hydrolysis is completed, the temperature is raised to terminate the reaction;

[0045] Filtration and concentration to obtain a hydrolyzate; the hydrolyzate is concentrated using a scraper concentrator to obtain a concentrated extract with a mass fraction of 35%;

[0046] Mixing and homogenization: Mix all ingredients evenly and use a high-pressure homogenizer to ensure a fine powder.

[0047] Compression into capsules: The homogenized powder is filled into hard capsules in an automatic capsule filling machine.

[0048] Inspection and Packaging: Quality inspection of finished capsules.

[0049] Specifically, increasing the content of vitamins C, A, and D not only supports daily immune function, but also helps to enhance bone and skin health, which is especially important for middle-aged and elderly people.

[0050] Example 3

[0051] A bovine bone protein peptide solid beverage, the raw material ratio includes: 35 parts of bovine bone protein peptide, 2 parts of wolfberry peptide powder, 30 parts of whey protein, 15 parts of maltodextrin, 5 parts of kudzu root extract, 0.5 parts of vitamin C, 0.5 parts of flavor (lemon flavor), 0.5 parts of sweetener (sucralose), 1 part of anti-caking agent (silicon dioxide), and the bovine bone protein peptide solid beverage preparation steps include:

[0052] Enzymatic hydrolysis and concentration: Follow steps 4 and 5 in the basic preparation method to obtain high-purity bovine bone protein peptide, namely:

[0053] Enzymatic hydrolysis, adding acidic protease at a mass ratio of 1% in sequence, adjusting the pH to 7 after enzymatic hydrolysis for 4 hours, and then adding neutral protease at a mass ratio of 1% for enzymatic hydrolysis for 3 hours; after the enzymatic hydrolysis is completed, the temperature is raised to terminate the reaction;

[0054] Filtration and concentration to obtain a hydrolyzate; the hydrolyzate is concentrated using a scraper concentrator to obtain a concentrated extract with a mass fraction of 35%;

[0055] Mixing and Homogenizing: All ingredients are mixed and processed using a high pressure homogenizer to ensure a homogeneous formulation.

[0056] Drying and Packaging: The mixture is converted into powder using spray drying technology and then packaged into a single serving of solid beverage.

[0057] Specifically, increasing the proportion of enzymatic bone powder and increasing the amount of enzymatic bone powder can help enhance bone health and have significant effects in increasing bone density and preventing osteoporosis.

[0058] Example 4

[0059] The preparation steps are the same as those in Example 1, except that the raw material ratio includes: 15 parts of bovine bone polypeptide, 0.85 parts of type II collagen peptide, 0.15 parts of casein phosphopeptide, 40 parts of whey protein, 5 parts of soy protein isolate, 3.5 parts of enzymatic bone powder, 22.56 parts of maltodextrin, 0.375 parts of vitamins A, D, and C, 10 parts of oligofructose, 0.750 parts of elderberry juice powder, 0.05 parts of turmeric, 0.005 parts of black pepper extract, 1 part of flavor, 0.75 parts of xanthan gum, and 0.01 parts of sucralose.

[0060] Specifically, the addition of black pepper extract improves the absorption efficiency of nutrients (especially the active ingredients in turmeric), and also has anti-inflammatory effects, which is beneficial to joint health.

[0061] Experimental Example 1

[0062] Assessment of joint health and inflammation

[0063] Experimental Grouping

[0064] Experimental subjects: 60 healthy male SD rats, weighing 200-250g, were randomly divided into 3 groups, with 20 rats in each group.

[0065] Control group: given basic feed without any treatment.

[0066] Experimental Group 1: The mice were given a basic feed and a standard bovine bone protein peptide composition, namely, the bovine bone protein peptide composition in Example 1, was added.

[0067] Experimental Group 2: The mice were given a basic feed and supplemented with the bovine bone protein peptide composition of Example 4 (containing new ingredients that enhance anti-inflammatory effects, turmeric extract and black pepper extract).

[0068] Induces arthritis

[0069] Model establishment: Arthritis was induced by injecting 0.1 mL of complete Freund's adjuvant (CFA) into the left hind ankle joint of rats, and experimental treatment was started 7 days later.

[0070] Experimental treatment

[0071] Treatment cycle: 28 consecutive days, once a day.

[0072] The experimental groups 1 and 2 were orally administered with 0.2 g / kg body weight of bovine osteoproteinase, and the control group was given normal saline.

[0073] Measurement and evaluation

[0074] Measurement of joint swelling: The diameter of the left hind ankle joint of each group of rats was measured using a vernier caliper before treatment, 14 days after treatment, and 28 days after treatment.

[0075] Joint mobility scores: The joint mobility of rats was assessed by behavioral testing, with scores ranging from 0 (no movement) to 100 (full movement), before treatment and after 28 days of treatment.

[0076] Determination of inflammatory markers in blood: Orbital blood samples were collected from rats, and the concentrations of C-reactive protein (CRP) and tumor necrosis factor α (TNFα) in serum were determined by ELISA.

[0077] Histological analysis of joint tissue: After the experiment, the rats were killed and the left hind ankle joint was taken for tissue sectioning. The inflammation of the joint tissue was observed under a microscope after H&E staining, and the inflammation score was calculated.

[0078] Data analysis

[0079] Statistical methods: SPSS software was used to analyze the data, and one-way analysis of variance (ANOVA) was used to compare the differences among the groups. A P value < 0.05 was considered to be statistically significant.

[0080] Experimental Results

[0081] Joint swelling measurement:

[0082] Control group: The average joint diameter increased from an initial 3.5 mm to 4.5 mm.

[0083] Experimental group 1: increased from 3.5mm to 3.9mm.

[0084] Experimental Group 2: increased from 3.5mm to 3.7mm.

[0085] Statistical analysis: Experimental group 2 showed a significant reduction in joint swelling compared to the control group and experimental group 1 (p<0.05).

[0086] Joint mobility score:

[0087] Control group: The average score dropped from an initial 90 to 70.

[0088] Experimental group 1: decreased from 90 to 85.

[0089] Experimental group 2: decreased from 90 to 88.

[0090] Statistical analysis: The joint mobility of experimental group 2 was significantly better than that of the control group and experimental group 1 (p<0.05).

[0091] Inflammatory markers in the blood:

[0092] Control group: CRP increased to 20 mg / L, TNF-α increased to 250 pg / mL.

[0093] Experimental group 1: CRP increased to 15 mg / L, TNF-α increased to 200 pg / mL.

[0094] Experimental group 2: CRP increased to 10 mg / L, TNF-α increased to 150 pg / mL.

[0095] Statistical analysis: The levels of CRP and TNF-α in experimental group 2 were significantly lower than those in the other two groups (p<0.01).

[0096] Histological analysis of joint tissue:

[0097] Control group: average inflammation score was 2.8.

[0098] Experimental group 1: average inflammation score 2.0.

[0099] Experimental group 2: average inflammation score 1.2.

[0100] Statistical analysis: The inflammation score of experimental group 2 was significantly lower than that of the control group and experimental group 1 (p<0.01).

[0101] in conclusion

[0102] Experimental data showed that the anti-inflammatory formula in Experimental Group 2 significantly reduced joint swelling, improved joint mobility, and significantly reduced inflammatory markers in the blood and tissue inflammation scores.

[0103] Experimental Example 2

[0104] Bone density and osteoporosis prevention

[0105] Experimental groups:

[0106] Experimental subjects: Sixty 8-week-old female SD rats were randomly divided into 3 groups, with 20 rats in each group.

[0107] Control group: given basic feed without any treatment.

[0108] Experimental Group 1: The mice were given a basic feed and a standard bovine bone protein peptide composition, namely, the bovine bone protein peptide composition in Example 1, was added.

[0109] Experimental Group 2: The mice were given a basic feed and supplemented with the bovine bone protein peptide composition of Example 4 (an enhanced formula containing enzymatically hydrolyzed bone meal and casein phosphopeptide).

[0110] Establishment of osteoporosis model

[0111] Model establishment: Osteoporosis was induced in rats by bilateral ovariectomy (OVX). Experimental treatment began one week after surgery.

[0112] Experimental treatment

[0113] Treatment period: 12 consecutive weeks, once a day.

[0114] Experimental groups 1 and 2 were both orally administered with 0.3 g / kg body weight of bovine bone protein peptide, and the control group was given an equal amount of normal saline.

[0115] Measurement and evaluation

[0116] Bone mineral density measurement: Dual-energy X-ray absorptiometry (DXA) was used to measure the femur bone mineral density (BMD) of rats in each group before the experiment, and after 6 and 12 weeks of the experiment.

[0117] Bone microstructure analysis: After the experiment, the rats were killed and the femurs were taken for micro-CT scanning to analyze the number, thickness and interstitial microstructure parameters of trabeculae.

[0118] Determination of calcium and phosphorus levels in blood: At the end of the experiment, orbital blood samples were collected from rats to determine the concentrations of calcium (Ca) and phosphorus (P) in serum.

[0119] Histological analysis of bone tissue: The femur was decalcified and tissue sections were prepared. The bone tissue structure was observed under a microscope after H&E staining.

[0120] Data analysis

[0121] Statistical methods: SPSS software was used to analyze the data, and one-way analysis of variance (ANOVA) was used to compare the differences among the groups. A P value < 0.05 was considered to be statistically significant.

[0122] Experimental Results

[0123] Bone density measurement:

[0124] Control group: The average bone mineral density increased from 0.50 g / cm 2 Down to 0.40g / cm 2 .

[0125] Experimental group 1: from 0.50g / cm 2 Down to 0.48g / cm 2 .

[0126] Experimental group 2: from 0.50g / cm 2 Down to 0.49g / cm 2 .

[0127] Statistical analysis: The bone density of experimental group 2 was significantly higher than that of the control group (p<0.05).

[0128] Bone microstructure analysis:

[0129] Control group: Micro-CT showed multiple microstructural damages.

[0130] Experimental group 1: less microstructural damage.

[0131] Experimental Group 2: The microstructure is more intact and the damage is minimal.

[0132] Statistical analysis: The bone microstructural integrity of experimental group 2 was significantly better than that of the other two groups (p<0.01).

[0133] Calcium and phosphorus levels in the blood:

[0134] Control group: blood calcium 8.2 mg / dL, blood phosphorus 5.1 mg / dL.

[0135] Experimental group 1: blood calcium 8.5 mg / dL, blood phosphorus 5.3 mg / dL.

[0136] Experimental group 2: blood calcium 9.0 mg / dL, blood phosphorus 5.5 mg / dL.

[0137] Statistical analysis: The blood calcium and phosphorus levels in experimental group 2 were significantly higher than those in the control group and experimental group 1 (p<0.05).

[0138] in conclusion

[0139] Experimental data showed that the formula in Experimental Group 2 significantly increased bone density, improved bone microstructure, and increased blood calcium and phosphorus levels, indicating that it has potential benefits in preventing osteoporosis.

[0140] In summary, it can be seen that the present invention adopts a combined enzymatic hydrolysis process of acidic protease and neutral protease, and through intelligent enzyme cleavage site design, the generation rate of specific functional peptides (peptides that promote bone health and anti-inflammatory effects) is improved; the biological activity of the product is enhanced, especially in promoting bone density increase and relieving joint inflammation, thereby improving the therapeutic effect of the product on osteoporosis and arthritis; key functional ingredients such as active peptides, turmeric and black pepper extracts are protected by nano-encapsulation technology to prevent them from being destroyed during the production process, and promote the absorption and utilization of these ingredients in the body; the stability and bioavailability of the functional ingredients are improved, and the mechanism of enhanced absorption helps to more effectively promote bone calcium deposition and relieve joint inflammation; high-pressure homogenizer technology is used to ensure the uniform distribution of functional ingredients in the product, avoiding the problem of unstable efficacy caused by uneven distribution of ingredients; homogenization technology significantly improves the uniformity and stability of the product, ensuring that each dose of the product has a consistent therapeutic effect in improving osteoporosis and relieving arthritis.

[0141] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. A bovine bone protein peptide composition that can improve osteoporosis and relieve arthritis, characterized in that: The bovine bone protein peptide composition is prepared from the following raw materials in parts by weight: 20-45 parts of bovine bone protein peptide, 2-4 parts of soy protein isolate, 5-12 parts of oligofructose, 25-35 parts of whey protein, 15-20 parts of maltodextrin, 5-15 parts of kudzu root extract, 3-5 parts of bonito elastin peptide, 2-3 parts of enzymatic bone powder, 1-10 parts of nano pearl powder, and 0.8-2.8 parts of wolfberry peptide powder. , xanthan gum 0.7-0.9 parts, turmeric 0.5 parts-1.5 parts, elderberry juice powder 0.5-0.75 parts, vitamins A, D, C 0.4-0.45 parts, polygonatum peptide powder 0.05 parts-1 parts, casein phosphopeptide 0.05-0.2 parts, flavor 0.02-0.05 parts, sucralose 0.008-0.02 parts, black pepper extract 0.005-0.18 parts.

2. A bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis according to claim 1, characterized in that: The bovine bone protein peptide composition is prepared from the following raw materials in parts by weight: 25 parts of bovine bone protein peptide, 2 parts of soy protein isolate, 5.5 parts of fructooligosaccharide, 30 parts of whey protein, 18 parts of maltodextrin, 6 parts of kudzu root extract, 4 parts of bonito elastin peptide, 2.5 parts of enzymatic bone powder, 6.32 parts of nano pearl powder, 1 part of wolfberry peptide powder, 0.8 parts of xanthan gum, 1 part of turmeric, 0.8 parts of elderberry juice powder, 0.46 parts of vitamins A, D and C, 0.09 parts of polyanthocyanidin powder, 0.2 parts of casein phosphopeptide, 0.04 parts of flavor, 0.018 parts of sucralose and 0.16 parts of black pepper extract.

3. A method for preparing a bovine bone protein peptide composition that can improve osteoporosis and relieve arthritis, characterized in that: The method comprises the following steps: using the bovine bone protein peptide composition for improving osteoporosis and relieving arthritis as described in any one of claims 1 to 2: Step S1, degreasing cattle bones, crushing cattle bones into pieces of 1-5 cm in size and adding them into a degreasing tank; adding purified water into the degreasing tank at a ratio of 1:1, and boiling for 4 hours; lowering the temperature of the degreasing tank to 75°C, and the vacuum degree to -0.045MPa to -0.12MPa, and separating the upper layer of fat; boiling at normal pressure, and then continuing to add purified water into the degreasing tank at a ratio of 1:1, heating to 100°C, and boiling for 4 hours; and separating the upper layer of fat after standing; Step S2, acid treatment, the defatted cattle bones are placed in a hydrochloric acid solution with a mass fraction of 1.15% and immersed for 4 hours; Step S3, bone mud preparation, cattle bones are crushed and ground into bone mud, added into a 2.5% by mass vitamin C aqueous solution, and soaked for 8h-10h to obtain an enzymatic substrate; the usage ratio of cattle bones to vitamin C aqueous solution is 1kg:3.2L; Step S4, enzymatic hydrolysis, adding acidic protease for enzymatic hydrolysis; adjusting the pH to 7, adding neutral protease for enzymatic hydrolysis; after the enzymatic hydrolysis is completed, raising the temperature to terminate the reaction; Step S5, filtering and concentrating, filtering to obtain a hydrolyzate; Step S6, mixing with a high pressure homogenizer, mixing the concentrate and all other dry powder ingredients; using a high pressure homogenizer to process the mixture to form a uniform mixture; Step S7, embedding and drying, embedding and drying the homogenized mixed solution to prepare a granular bovine bone protein peptide composition.

4. The method for preparing a bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis according to claim 3, characterized in that: In step S2, the usage ratio of the cattle bone to the hydrochloric acid solution is 1 kg:3.2 L.

5. The method for preparing a bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis according to claim 4, characterized in that: In step S4, acidic protease is added in a mass ratio of 1%, and after enzymatic hydrolysis for 3-4 hours to adjust the pH to 7, neutral protease is added in a mass ratio of 1% and enzymatic hydrolysis is performed for 2-3 hours.

6. The method for preparing a bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis according to claim 5, characterized in that: In step S5, the hydrolyzate is concentrated by using a scraper concentrator to obtain a concentrated extract having a mass fraction of 35%.

7. The method for preparing a bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis according to claim 6, characterized in that: In step S6, other dry powder ingredients include: maltodextrin, vitamins, and extracts.

8. A use of the bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis as claimed in claim 1 in the preparation of health products, characterized in that: The health care products include bovine bone protein peptide beverage powder, bovine bone protein peptide capsules and bovine bone protein peptide solid beverage.

9. A health product containing the bovine bone protein peptide composition capable of improving osteoporosis and relieving arthritis as claimed in claim 1, characterized in that: The health care product comprises the bovine bone protein peptide composition according to any one of claims 1-2.

10. The health care product according to claim 9, characterized in that: The content of the bovine bone protein peptide composition in the health care product is 100%.

Images