The invention provides a medical adhesive prepared by adopting skin mucus of giant salamander as a raw material and a preparation method, relating to the medical field. The medical adhesive is prepared by adopting the skin mucus of giant salamander as the raw material. The preparation method comprises the following steps: A, carrying out quick-freezing, B, carrying out low-temperature sterilizing, C, carrying out low-temperature grinding, D, carrying out dehydrating, and E, forming styptic medicinal powder. The second-level bleeding-stopping and adhering functions of the skin mucus of giant salamander are utilized, so that the rapid bleeding stopping and adhering functions for the wound are realized; during the whole process of preparing the adhesive, the physical methods are only adopted, and the raw materials are pure natural biological materials, so that no toxic and side effects are caused; the prepared adhesive is wide in applicable people, realizes the second-level bleeding-stopping and adhering functions, and is good in effect, the pain of patients is alleviated, and the medical adhesive can be widely applied to the medical fields of surgical operation, traumatic wound healing, feature beautifying, and the like. In addition, for the adult giant salamander, 0.3-0.6 kg of skin mucus can be obtained from each adult giant salamander each month, and with the prepared medical adhesive, the additional value for culturing giant salamander can be greatly improved.