12 results about "Giant salamander" patented technology

This application belongs to the field of biotechnology, specifically relating to giant salamander skin collagen peptides, their preparation methods, and applications. This application provides a method for preparing giant salamander skin collagen peptides. The method first pre-treats the fish skin, then performs ultrasonic-assisted extraction under acidic conditions to effectively promote collagen dissolution. The extract undergoes flocculation to remove impurities, significantly reducing interference from other proteins. The enzymatic hydrolysis stage employs a stepwise enzymatic hydrolysis strategy: first, a combination of alkaline protease and another specific protease is used for hydrolysis, sequentially cleaving the complex structure of giant salamander skin collagen to improve initial hydrolysis efficiency and generate specific intermediate peptides; subsequently, a flavor protease is added for further deep hydrolysis to achieve complete hydrolysis. This method has high enzymatic hydrolysis efficiency, controllable hydrolysis degree, concentrated molecular weight distribution of the obtained collagen peptides, high yield of the target product, and stable process.

The application discloses a kind of embroidery needle method for showing the special body shape longitudinal fish scale of Wuchang fish, it is related to embroidery technical field, specifically a kind of embroidery needle method for showing the special body shape longitudinal fish scale of Wuchang fish, its steps include: first, the pattern designed according to the body shape of Wuchang fish is embroidered on the cloth, and the pattern is embroidered around using silver wire and colored silk thread, from the tail of fish, silver wire is wound into the ring with a diameter of 1-2mm, and the silk thread is fixed, then, it is repeated longitudinally to the direction of fish head, and is overlapped with the previous ring, forming the circular embroidery mark arranged longitudinally, simulating the longitudinal texture of fish scale, and the three-dimensional sense of decorative needle method is improved by embroidering from the tail of fish to the direction of fish head. The method can accurately show the characteristics of the special body shape and longitudinal fish scale of Wuchang fish, and is suitable for embroidery art, clothing and home accessories manufacturing.

This invention discloses a method for detecting the freshness of giant salamander meat based on color constancy and integrated intelligent vision, belonging to the fields of food safety and computer vision technology. The method includes: acquiring RAW images of the meat sample and an indicator film and resolving them into linear radiosity tensors; constructing a color correction model using a latent diffusion local redraw network and a conditional control network; synthesizing a structure-constrained virtual reference color card in a masked region; extracting ambient light source features and performing adaptive white balance compensation to output a standard color image; inputting this image into a deep network optimized by ArcFace to extract a high-dimensional feature vector; and finally inputting this vector into an XGBoost model to fit a nonlinear decision boundary and outputting a freshness rating. This invention effectively reduces color shift interference from complex light sources, improves the recognition accuracy of samples in the critical period of color change, and provides a highly robust solution for non-destructive monitoring of giant salamander meat freshness in actual market environments.

This invention discloses an anti-aging active polypeptide composition, its preparation method, and its application. The anti-aging active polypeptide composition comprises the following components in parts by weight: 35-45 parts of collagen active tripeptide, 15-25 parts of artificially bred giant salamander peptide, 20-30 parts of ray peptide, and 15-25 parts of type III collagen peptide. Furthermore, the purity of each individual component of the collagen active tripeptide, artificially bred giant salamander peptide, ray peptide, and type III collagen peptide is ≥95%. This invention effectively solves the problems of low raw material purity, poor absorption, single efficacy, and insufficient stability in existing technologies by compounding the collagen active tripeptide, artificially bred giant salamander peptide, ray peptide, and type III collagen peptide in specific parts by weight and strictly controlling the purity of each individual component to be no less than 95%. This results in a high-purity, high-absorption, multi-target synergistic, and stable anti-aging polypeptide composition.

This invention relates to the field of food processing technology, and in particular to a highly stable high internal phase emulsion based on giant salamander mucus, its preparation method, and its application. The method includes the following steps: (1) mixing and stirring ascorbic acid solution and freeze-dried giant salamander mucus powder, centrifuging, and taking the supernatant as the aqueous phase of the high internal phase emulsion; (2) mixing the oil phase with the aqueous phase obtained in step (1), and homogenizing to obtain a highly stable high internal phase emulsion based on giant salamander mucus. The method of this invention is simple and mild, opening up new avenues for the high-value utilization of giant salamander aquaculture byproducts. The obtained high internal phase emulsion, due to its natural, stable nature and unique rheological properties, has broad application potential in the food industry, cosmetics, and pharmaceutical carrier fields.

This invention belongs to the field of biomaterials technology, specifically relating to a bioactive scaffold, its preparation method, and its applications. This invention incorporates functionalized mesoporous silica nanoparticles loaded with aminoguanidine into the skin secretions of the giant salamander and polycaprolactone electrospun fibers to construct a bioactive composite scaffold (SPMA). This scaffold enables the sustained release of AG and osteogenic factors, effectively inhibiting free radical generation, AGEs toxicity, and inflammatory responses. Furthermore, it significantly improves the survival rate, osteogenic differentiation, and mineralization capacity of bone marrow mesenchymal stem cells in a high-glucose microenvironment. In vivo experiments further demonstrate that in a streptozotocin-induced diabetic mouse model of skull defects, the SPMA scaffold can alleviate local inflammation, promote angiogenesis, and significantly accelerate new bone formation and defect repair.

The utility model provides a kind of fisher for gavial breeding, belong to the field of aquaculture fisher, including connecting rib and the telescopic handle being set on connecting rib, connecting rib side wall is connected with fishing net, and the inner side wall of fishing net is equipped with anti-escape net, the outer side wall of anti-escape net is equipped with multiple first elastic support ribs and second elastic support ribs, and one end of multiple first elastic support ribs and second elastic support ribs is connected with the inner side wall of fishing net, the utility model is equipped with anti-escape net by setting rounded table, and the tapered inlet design can use gavial advancing habit to prevent its retreat, when gavial struggles, elastic support rib extrudes anti-escape net, so as to contract escape space, so gavial cannot slip from fishing net.

The application relates to the field of biomaterials, and particularly relates to application of a giant salamander mucus extract hydrolysate in preparation of an anti-dentin sensitivity agent. The anti-dentin sensitivity agent comprises a hydrolysate retaining the biological activity of SSAD, the hydrolysate being a product of reduction of the giant salamander mucus extract by an acid solution with a pH value of 1-5, and the product comprising five active proteins, namely MHC class I molecule alpha chain, homeobox protein Hox-D13, abnormal heavy chain 3 of axonemal dynein, ATP synthase protein and cytochrome b; the particle size of the giant salamander mucus extract is 1-50 mu m; and the pH value of the anti-dentin sensitivity agent is 5.5-7.5. The giant salamander mucus extract is treated by the acid solution to obtain the hydrolysate containing the multiple active proteins, the hydrolysate can effectively promote deep closure of dentin tubules, and has long-term stable biocompatibility; the pH value is controlled to ensure that the biological activity is completely retained, and the curative effect of the anti-dentin sensitivity agent is remarkably improved.

This invention belongs to the field of biomedical materials and discloses a non-denatured collagen from the giant salamander, its extraction method, and its pharmaceutical application in promoting wound healing. The non-denatured collagen is obtained from giant salamander skin powder using an acetic acid extraction system or a pepsin-assisted acetic acid extraction system. It maintains a triple helix structure, exhibits a positive peak near 221 nm and a negative peak near 197 nm in circular dichroism spectroscopy, and a denaturation peak at 54–59°C in DSC. This collagen significantly promotes repair in a zebrafish tail fin injury model and can be used to prepare drugs or topical repair materials that promote wound healing.

This application relates to the field of molecular compound composition technology, specifically disclosing a composition of a polymeric compound containing giant salamander peptides and its preparation method. The composition of a polymeric compound containing giant salamander peptides comprises the following components: a giant salamander small molecule protein peptide-carboxymethyl chitosan graft copolymer, hyaluronic acid, nano-zinc oxide, gallic acid, and phosphate buffer. The preparation method is as follows: hyaluronic acid is dispersed in phosphate buffer, stirred, and then the giant salamander small molecule protein peptide-carboxymethyl chitosan graft copolymer is added. After mixing evenly, nano-zinc oxide is added, and finally gallic acid is added and stirred to obtain the final product. This application achieves stable linkage between giant salamander small molecule protein peptides and carboxymethyl chitosan while maximally maintaining the natural conformation and biological activity of the giant salamander small molecule protein peptides, and exhibits excellent and stable synergistic effects.

This application relates to the technical field of peptide separation and purification, and in particular to a method for preparing giant salamander protein peptides using a simulated moving bed purification process. The method includes: extracting and enzymatically hydrolyzing pulverized giant salamander to obtain a precursor hydrolysate; adding a polyanionic compound to the precursor hydrolysate to lower the pH, using an interlocking cutoff control based on a zeta potential of zero, a critical pH value, and a jump point in the second derivative of absorbance, until a third separation solution is obtained at a zeta potential of 0±2 mV and a pH of 4.30±0.05; introducing the third separation solution into a simulated moving bed to obtain a target peptide eluent; flash evaporating the target peptide eluent to remove water and low-boiling alcohols, then adding tert-butanol to the concentrate and cryogenically sublimating and drying it, causing the tert-butanol to crystallize and transform into a porous ice crystal template, thus obtaining the giant salamander protein peptide. This application can solve the defect of electrostatic shielding leading to competitive adsorption of heteropeptides, achieving stable mass transfer and high-purity recovery.

This invention discloses a colon-targeting capsule, its preparation method, and its application, belonging to the field of biomedical materials technology. The colon-targeting capsule provided by this invention comprises a capsule shell and contents encapsulated by the capsule shell; the capsule shell is a colon-targeting capsule shell; the raw materials for preparing the contents include regenerated powder and a small molecule acid solution; the regenerated powder is a depolymerization product of the mucus from the skin of the giant salamander. The colon-targeting capsule provided by this invention exhibits good storage stability and batch stability, can target colonic release, and achieves synergistic regulation of the "mucus barrier—microecology—mucosal homeostasis." This invention also provides a preparation method and application of the above-mentioned colon-targeting capsule.