50 results about "Giant salamander" patented technology

The invention relates to the technical field of giant salamander by-product quality evaluation, and discloses a giant salamander by-product source functional factor quality evaluation system and method, and the method comprises the steps: obtaining a giant salamander by-product sample, carrying out the pretreatment, and extracting a functional factor to obtain a to-be-detected sample; identifying and quantitatively determining specific markers based on biochemical analysis and molecular detection means to generate a marker data set; inputting the marker data into a multi-modal feature fusion module, extracting activity and stability features, and performing time sequence dynamic modeling; activity and stability independent evaluation branches are set, and corresponding score values are generated; dividing functional domains according to functional factor categories, constructing an evaluation curve based on historical data, substituting a target sample into each functional domain and an adjacent functional domain to generate functional domain evaluation results, and performing weighted fusion; and finally, carrying out weighted integration on the activity score, the stability score and the comprehensive evaluation value, and determining a final quality evaluation result.

The invention relates to the technical field of giant salamander peptide separation and purification, in particular to a giant salamander peptide extraction and separation and purification method and system.The method comprises the steps that healthy giant salamanders and giant salamander processing equipment are determined, giant salamander processing by-products are obtained, the giant salamander processing by-products are pretreated, to-be-processed by-products are obtained, the to-be-processed by-products are degreased and deodorized, and the giant salamander peptide is obtained. Preparing giant salamander protein dry powder, preparing giant salamander crude peptide liquid, obtaining a target peptide fragment, finely separating the target peptide fragment to obtain target active peptide distillate, freeze-drying the target active peptide distillate to obtain high-purity giant salamander peptide powder, and storing the molecular weight, amino acid sequence and activity verification parameters of the peptide fragment. And extracting, separating and purifying the giant salamander peptide based on the optimized storage database and the giant salamander grease. The extraction efficiency and the separation and purification efficiency of the giant salamander peptide can be improved.

The invention relates to the technical field of automatic identification of animal behaviors, in particular to an automatic identification method for pulmonary respiration behaviors of giant salamanders, and the method comprises the steps: obtaining an initial video image of a target region; preprocessing the initial video image to obtain a standard video image; performing feature enhancement on the standard video image to obtain an enhanced feature image; performing multi-scale respiratory behavior feature extraction on the enhanced feature image to obtain depth feature maps of different scales; performing multi-level feature aggregation on the depth feature maps of different scales, and generating an aggregated feature map; and generating an identification result based on the aggregated feature map. According to the method, the problems that the omission ratio of manual monitoring of the breathing behavior in a night low-contrast scene is high and long-term efficient and objective monitoring is difficult to realize due to night walking and cave habits of the giant salamander are solved.

The utility model discloses giant salamander amino acid nutrient solution filling and sealing equipment, which belongs to the technical field of filling equipment and comprises a conveying seat, a plurality of conveying grooves are formed in the top surface of the conveying seat, conveying assemblies are arranged in the conveying grooves, sealing bottles are placed on the top surfaces of the conveying assemblies, and the front side of the conveying seat is connected with a cylinder body of a lifting cylinder. Piston rods of the two lifting air cylinders are vertically and upwards connected with a liquid filling mechanism, the rear side of the conveying base is connected with column bodies of hollow columns, a cover sealing mechanism is arranged between the two hollow columns, and limiting assemblies are arranged behind the liquid filling mechanism and the cover sealing mechanism. According to the device, a plurality of sealing bottles are simultaneously conveyed to the position below the liquid filling mechanism for liquid filling through the conveying seat, then the sealing bottles filled with liquid are uniformly conveyed to the position below the capping mechanism for capping and sealing, manual assistance is not needed in the whole process, and the device has the advantages of being compact in structure and high in efficiency and is suitable for large-scale flow line production.

The invention relates to the technical field of grinding extraction, and provides a preparation method and application of a giant salamander mucus active extract based on liquid nitrogen grinding, and the preparation method of the giant salamander mucus active extract comprises the following steps: electrically stimulating the body surface of a giant salamander, and collecting giant salamander mucus; the collected giant salamander mucus is pre-cooled and then freeze-dried at the temperature of-80 DEG C, and a mucus freeze-dried substance is obtained; carrying out liquid nitrogen grinding on the mucus freeze-dried substance, and continuously obtaining grinding images within a preset time at the same time; grinding parameters are obtained based on the grinding image; mixing and stirring the ground powder and water to obtain an extract mixed solution; centrifuging and filtering the extract mixed solution to obtain extract filtrate; and pre-cooling the extract filtrate at-80 DEG C, and freeze-drying to obtain the giant salamander mucus active extract. The method improves the active content of the giant salamander mucus active extract.

1. Name of the design product: bath thermometer (giant salamander). 2. Use of the design product: thermometer for measuring water temperature when children take a bath, and also toy. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The present invention discloses a rosa roxburghii tratt fruit peptide drink and a preparation method thereof, and relates to the technical field of nutritional health drink processing, and the rosa roxburghii tratt fruit peptide drink consists of the following raw materials in parts by mass: 100 parts of rosa roxburghii tratt fruit juice, 1.5-2.5 parts of a lucid ganoderma extract, 0.8-1.2 parts of a radix astragali seu hedysari extract, 4.5-7.5 parts of a coix seed extract, 8-12 parts of giant salamander peptide, 4.0-6.5 parts of locust bean gum, 0.03-0.05 part of stevioside and 60-100 parts of water. According to the rosa roxburghii tratt peptide beverage and the preparation method thereof, the rosa roxburghii tratt is scientifically compounded with various medicinal and edible substances and nutritional ingredients such as the lucid ganoderma, the astragalus membranaceus, the coix seeds and the giant salamander peptide for the first time, and a nutrition complementary composite system is formed. The compounding of lucid ganoderma and roxburgh rose has a scientific basis supported by literatures.

This application belongs to the field of biotechnology, specifically relating to giant salamander skin collagen peptides, their preparation methods, and applications. This application provides a method for preparing giant salamander skin collagen peptides. The method first pre-treats the fish skin, then performs ultrasonic-assisted extraction under acidic conditions to effectively promote collagen dissolution. The extract undergoes flocculation to remove impurities, significantly reducing interference from other proteins. The enzymatic hydrolysis stage employs a stepwise enzymatic hydrolysis strategy: first, a combination of alkaline protease and another specific protease is used for hydrolysis, sequentially cleaving the complex structure of giant salamander skin collagen to improve initial hydrolysis efficiency and generate specific intermediate peptides; subsequently, a flavor protease is added for further deep hydrolysis to achieve complete hydrolysis. This method has high enzymatic hydrolysis efficiency, controllable hydrolysis degree, concentrated molecular weight distribution of the obtained collagen peptides, high yield of the target product, and stable process.

The application discloses a kind of embroidery needle method for showing the special body shape longitudinal fish scale of Wuchang fish, it is related to embroidery technical field, specifically a kind of embroidery needle method for showing the special body shape longitudinal fish scale of Wuchang fish, its steps include: first, the pattern designed according to the body shape of Wuchang fish is embroidered on the cloth, and the pattern is embroidered around using silver wire and colored silk thread, from the tail of fish, silver wire is wound into the ring with a diameter of 1-2mm, and the silk thread is fixed, then, it is repeated longitudinally to the direction of fish head, and is overlapped with the previous ring, forming the circular embroidery mark arranged longitudinally, simulating the longitudinal texture of fish scale, and the three-dimensional sense of decorative needle method is improved by embroidering from the tail of fish to the direction of fish head. The method can accurately show the characteristics of the special body shape and longitudinal fish scale of Wuchang fish, and is suitable for embroidery art, clothing and home accessories manufacturing.

This invention discloses a compound composition of giant salamander peptide extract and its application in sleep-promoting products. The giant salamander meat is hydrolyzed with papain at 45-55°C, followed by hydrolysis with a complex protease, and the enzymes are inactivated after hydrolysis. The mixture is then filtered through a ceramic membrane and adsorbed using activated carbon to obtain the giant salamander peptide extract. The giant salamander peptide extract is compounded with casein peptide, tea theanine, daylily extract, poria cocos extract, jujube seed extract, lily powder, and γ-aminobutyric acid (GABA) to obtain a compound composition. Applying this compound composition to sleep-promoting products can enhance their sleep-aiding effects.

This invention discloses a method for detecting the freshness of giant salamander meat based on color constancy and integrated intelligent vision, belonging to the fields of food safety and computer vision technology. The method includes: acquiring RAW images of the meat sample and an indicator film and resolving them into linear radiosity tensors; constructing a color correction model using a latent diffusion local redraw network and a conditional control network; synthesizing a structure-constrained virtual reference color card in a masked region; extracting ambient light source features and performing adaptive white balance compensation to output a standard color image; inputting this image into a deep network optimized by ArcFace to extract a high-dimensional feature vector; and finally inputting this vector into an XGBoost model to fit a nonlinear decision boundary and outputting a freshness rating. This invention effectively reduces color shift interference from complex light sources, improves the recognition accuracy of samples in the critical period of color change, and provides a highly robust solution for non-destructive monitoring of giant salamander meat freshness in actual market environments.

The present application provides a viscosity controllable chitosan-giant salamander skin secretion hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedical materials.The giant salamander skin secretion lyophilized powder is mixed and stirred with ascorbic acid solution respectively to obtain a giant salamander skin secretion solution and a chitosan solution; the giant salamander skin secretion solution and the chitosan solution are mixed in a mass ratio of 6-18:1 to obtain the viscosity controllable chitosan-giant salamander skin secretion hydrogel. The viscosity of the hydrogel can be effectively controlled, and the rheological properties of the hydrogel can be controlled by adjusting the mass ratio of the giant salamander skin secretion solution and the chitosan solution. The chitosan-giant salamander skin secretion hydrogel prepared by the present application has good physical properties, antibacterial hemostatic performance and biocompatibility, and can be widely used in the field of wound healing.

A segmented extraction device for a nutrient solution used in giant salamander protein production, which belongs to the technical field of biological extraction, and comprises a casing assembly (1), a

The invention relates to the technical field of animal grease extraction, in particular to an extraction process of giant salamander grease rich in EPA and DHA, which comprises the following steps: step 1, raw material refining pretreatment: selecting tail adipose tissues of cultured giant salamanders' second filial generation 'and offspring thereof meeting the GB2733 standard, and carrying complete inspection and quarantine proof, and legitimacy and safety of the raw materials are ensured. According to the method disclosed by the invention, the total extraction rate of the giant salamander tail grease oil is greater than or equal to 76% through a synergistic process of low-temperature composite enzymolysis activation, gradient temperature control vacuum decoction and secondary short-time oil extraction of residues, is improved by 13.18% compared with 68.61% of an original vacuum decoction scheme, and is far higher than 3%-5% of a conventional improvement in the field; and the residual oil rate is reduced from 8-10% of the original scheme to less than or equal to 3%, and the reduction amplitude reaches 77.5%, so that the full utilization of oil resources is realized, and the economic benefits of industrial production are greatly improved.

The invention provides a preparation method of giant salamander peptide, and belongs to the technical field of bioactive peptide preparation. The quality of the giant salamander peptide is greatly improved through low-temperature stress treatment before slaughtering, variable-pressure boiling, beta-cyclodextrin and sequential addition of neutral protease, papain, bromelain and the like, the finally obtained giant salamander peptide is high in small molecule peptide proportion and low in bitter taste, and the biological activity is remarkably superior to that of a product prepared by a traditional method; the method is very suitable for developing high-end functional food and health care products.

The present application relates to the technical field of bioactive peptides, and specifically discloses a preparation method and application of giant salamander hydrolyzed collagen active peptide. The amino acid sequence of the giant salamander hydrolyzed collagen active peptide is SPPRARV. The single polypeptide in giant salamander peptide powder is separated and identified by combining gel chromatography column and preparative chromatography for the first time, and the giant salamander hydrolyzed collagen active peptide with the amino acid sequence of SPPRARV is successfully obtained. The polypeptide is modified by using molecular docking technology, and the obtained modified peptide has good transdermal ability and cell repair ability, can promote cell scratch repair and cell proliferation, promote the increase of type I collagen content, and is suitable for skin repair and anti-aging medical and beauty products or skin damage repair drugs.

The invention relates to the technical field of traditional Chinese medicine compositions, and particularly discloses a medicinal and edible composition containing andrias davidianus peptide for dispelling effects of alcohol and protecting liver and a preparation method of the medicinal and edible composition. The anti-alcohol and liver-protecting medicinal and edible composition containing the giant salamander peptide comprises the giant salamander peptide and a traditional Chinese medicine compound extract, and the traditional Chinese medicine compound extract is prepared by extracting the following raw materials through a dual solvent system of alcohol extraction and water extraction: green tea, turmeric, mulberry leaves, hawthorn fruits, kudzuvine roots, hovenia dulcis thunb, poria cocos, cassia seeds, lotus leaf powder, amomum tsao-ko, chrysanthemums, pueraria flowers and white hyacinth beans. The anti-alcohol and liver-protecting medicinal and edible composition containing the giant salamander peptide can effectively avoid possible negative interaction between part of traditional Chinese medicine ingredients and the giant salamander peptide, the activity and the absorption and utilization rate of the composition are guaranteed, then the medicine effect is remarkably enhanced, the anti-alcohol and liver-protecting effect far close to the expected anti-alcohol and liver-protecting effect is achieved, and finally the anti-alcohol and liver-protecting medicinal and edible composition containing the giant salamander peptide with the good application quality is obtained.

This invention discloses an anti-aging active polypeptide composition, its preparation method, and its application. The anti-aging active polypeptide composition comprises the following components in parts by weight: 35-45 parts of collagen active tripeptide, 15-25 parts of artificially bred giant salamander peptide, 20-30 parts of ray peptide, and 15-25 parts of type III collagen peptide. Furthermore, the purity of each individual component of the collagen active tripeptide, artificially bred giant salamander peptide, ray peptide, and type III collagen peptide is ≥95%. This invention effectively solves the problems of low raw material purity, poor absorption, single efficacy, and insufficient stability in existing technologies by compounding the collagen active tripeptide, artificially bred giant salamander peptide, ray peptide, and type III collagen peptide in specific parts by weight and strictly controlling the purity of each individual component to be no less than 95%. This results in a high-purity, high-absorption, multi-target synergistic, and stable anti-aging polypeptide composition.

This invention relates to the field of food processing technology, and in particular to a highly stable high internal phase emulsion based on giant salamander mucus, its preparation method, and its application. The method includes the following steps: (1) mixing and stirring ascorbic acid solution and freeze-dried giant salamander mucus powder, centrifuging, and taking the supernatant as the aqueous phase of the high internal phase emulsion; (2) mixing the oil phase with the aqueous phase obtained in step (1), and homogenizing to obtain a highly stable high internal phase emulsion based on giant salamander mucus. The method of this invention is simple and mild, opening up new avenues for the high-value utilization of giant salamander aquaculture byproducts. The obtained high internal phase emulsion, due to its natural, stable nature and unique rheological properties, has broad application potential in the food industry, cosmetics, and pharmaceutical carrier fields.

The invention relates to the technical field of microbial fermentation engineering, and discloses a giant salamander fermentation product preparation method and system based on multi-parameter monitoring, and the method comprises the steps: receiving a preparation instruction, determining a giant salamander sample and a fermentation parameter set based on the preparation instruction, and obtaining an initial fermentation weight set, determining a target fermentation weight set based on a giant salamander sample and the initial fermentation weight set, calculating a frequency node by using the target fermentation weight set, performing fermentation operation on the giant salamander sample by using a preset fermentation condition, and judging the fermentation state of the giant salamander sample in the fermentation period by using a monitored pH value set, a monitored temperature value set and a monitored dissolved oxygen set, and adjusting the fermentation conditions within the adjustment time period to obtain updated fermentation conditions, and continuing to perform fermentation operation on the fermented giant salamander sample by using the updated fermentation conditions until the fermentation state is a qualified state, thereby realizing preparation of the giant salamander fermentation product. The preparation efficiency of the giant salamander fermentation product can be improved.

The application provides a giant salamander skin non-denatured collagen hydrogel dressing and a preparation method thereof. The giant salamander skin is used as a raw material, only sodium carbonate degreasing and mild pepsin extraction are performed, and then the giant salamander skin non-denatured collagen is obtained. The giant salamander skin non-denatured collagen is compounded with acceptable hydrogel accessories to form a hydrogel dressing with suitable swelling rate and degradability, so that excellent skin repair and anti-aging effects can be achieved without adding exogenous growth factors, and the dressing formula is greatly simplified. Compared with a traditional collagen extraction process, the process is simple, the cost is low, the collagen yield is much higher than that of traditional acid extraction and enzyme extraction methods, and the collagen retention rate can reach more than 90%. Furthermore, the high-value utilization of the giant salamander processing by-products is realized, the raw material source of the functional collagen is expanded, and the economic and environmental values are double.

This invention belongs to the field of biomaterials technology, specifically relating to a bioactive scaffold, its preparation method, and its applications. This invention incorporates functionalized mesoporous silica nanoparticles loaded with aminoguanidine into the skin secretions of the giant salamander and polycaprolactone electrospun fibers to construct a bioactive composite scaffold (SPMA). This scaffold enables the sustained release of AG and osteogenic factors, effectively inhibiting free radical generation, AGEs toxicity, and inflammatory responses. Furthermore, it significantly improves the survival rate, osteogenic differentiation, and mineralization capacity of bone marrow mesenchymal stem cells in a high-glucose microenvironment. In vivo experiments further demonstrate that in a streptozotocin-induced diabetic mouse model of skull defects, the SPMA scaffold can alleviate local inflammation, promote angiogenesis, and significantly accelerate new bone formation and defect repair.

The invention discloses a highland barley and fructus lycii polypeptide beverage as well as a preparation method and application of the highland barley and fructus lycii polypeptide beverage. The formula comprises the following components in parts by weight: 5-15 parts of highland barley extract, 3-10 parts of lycium ruthenicum primary pulp, 3-10 parts of hippophae rhamnoides primary pulp, 2-8 parts of radix puerariae extract, 1-5 parts of giant salamander active peptide powder, 1-4 parts of oyster extract, 0.5-3 parts of ginseng powder, 0.1-1 part of compound amino acid, 0.5-2 parts of a natural sweetening agent, 0.05-0.3 part of a stabilizer and the balance of water. The invention relates to the technical field of health-care food and beverage processing, and has the beneficial effects that Tibet Plateau characteristic resources are systematically compatible with marine active peptide giant salamander peptide, traditional anti-alcohol medicinal materials, functional amino acid and trace element yeast, and the combination acts on the whole process of absorption-metabolism-excretion of alcohol metabolism in a multi-target manner, so that the anti-alcohol effect is achieved. And the liver is synergistically protected through multiple ways of resisting oxidation, resisting inflammation, protecting liver cells and the like.

The invention discloses a giant salamander-flavored wine process manufacturing method and a process flow, and relates to the technical field of giant salamander-flavored wine preparation, the giant salamander-flavored wine process manufacturing method comprises the following steps: putting artificially cultured second-generation giant salamanders and water into a saucepan according to a mass ratio of 1: 3-1: 5, and cooking for 2-3 hours under the condition of steam gauge pressure of 0.02-0.1 MPa to obtain a stock solution containing giant salamander protein hydrolysate; white granulated sugar and red granulated sugar are added into the stock solution, the total mass of the white granulated sugar and the red granulated sugar is 8%-15% of the mass of the stock solution, the mass ratio of the white granulated sugar to the red granulated sugar is 2: 1-4: 1, the mixture is heated to 95-100 DEG C and stirred for not less than 30 min, and homogeneous fermentation base solution is formed; cooling the homogeneous fermentation base solution to 30-35 DEG C to obtain a cooled fermentation base solution; transferring the cooled fermentation base solution into a food-grade stainless steel fermentation container which is disinfected by saturated steam at 121 DEG C for 30 minutes, inoculating saccharomyces cerevisiae which accounts for 0.1-0.3% of the mass of the fermentation base solution, sealing, and fermenting at 25-28 DEG C for 45-55 days to obtain fermentation liquor.

The invention discloses an accurate giant salamander feeding and feed preparing system based on demand and supply linkage, and relates to the giant salamander soft pellet feed feeding technology, the system comprises a low-temperature storage bin and an intelligent feeding assembly, the intelligent feeding assembly is used for obtaining quantitative feed from the low-temperature storage bin and delivering the feed to a target pool according to a control instruction, and the intelligent feeding assembly is used for feeding the feed to the target pool. A material receiving assembly and a layered vibration discharging assembly are arranged in the low-temperature storage bin, and the layered vibration discharging assembly comprises a plurality of discharging units vertically distributed in the low-temperature storage bin. According to the vibrating device, vibration and discharging are synchronous in a layered vibration and discharging mode, the material bearing table and the movable material frame generate continuous and moderate vibration, the vibration mode is different from a traditional external vibration mechanism, feed compaction cannot be accelerated, the adhesive force between soft pellet feed can be destroyed, and the fluidity of the feed is improved; and an arch bridge structure is prevented from being formed fundamentally, the material blocking problem is avoided, and smooth discharging is ensured.

The invention discloses sensitive skin face moisturizing essence and a preparation method thereof, and belongs to the technical field of essence. Comprising a phase A and a phase B, wherein the phase A comprises lychee seed extract, giant salamander collagen freeze-dried powder, aronia melanocarpa extract, lens culinaris seed extract, trollius chinensis bunge extract, prinsepia utilis royle extract, deionized water, a thickening agent, a humectant, allantoin and butanediol; a phase B: squalane, silicone oil, an emulsifier, an emollient and a vetiver extract; phase C: antioxidant, preservative and (daily use) essence; the lychee seed extract, the giant salamander collagen freeze-dried powder, the aronia melanocarpa extract, the lens culinaris seed extract, the trollius brachypetalus extract, the prinsepia utilis royle extract and the vetiver extract are added in the preparation of the essence, and through the synergistic compatibility effect of all the components, the essence has a good moisturizing effect, achieves the effect of moisturizing the skin, and is free of toxic and side effects. The sensitive skin repairing problem is improved.

The utility model provides a kind of fisher for gavial breeding, belong to the field of aquaculture fisher, including connecting rib and the telescopic handle being set on connecting rib, connecting rib side wall is connected with fishing net, and the inner side wall of fishing net is equipped with anti-escape net, the outer side wall of anti-escape net is equipped with multiple first elastic support ribs and second elastic support ribs, and one end of multiple first elastic support ribs and second elastic support ribs is connected with the inner side wall of fishing net, the utility model is equipped with anti-escape net by setting rounded table, and the tapered inlet design can use gavial advancing habit to prevent its retreat, when gavial struggles, elastic support rib extrudes anti-escape net, so as to contract escape space, so gavial cannot slip from fishing net.

The invention relates to the field of biological materials, in particular to application of giant salamander skin secretions in preparation of a bone repair material. The invention provides a preparation method of a bone defect repair scaffold material, and is characterized in that the method comprises the following steps: S101, obtaining first bio-ink; s102, obtaining second biological ink; s103, obtaining third bio-ink; s104, the third bio-ink is placed in a container of an extrusion type bio-printer, and the platform temperature of a forming platform is kept at 15-20 DEG C; and S105, printing the bone defect repair stent material with a preset shape at the printing speed of 3-4 mm / s and the extrusion air pressure of 0.2-0.3 bar. According to the invention, the bone defect repair scaffold material is prepared by using an extrusion type biological printing technology. The prepared bone defect repair scaffold material is high in structural strength and has an excellent osteogenesis effect.

The application provides a preparation method of giant salamander skin in-situ collagen hydrogel dressing and application thereof in promoting burn wound healing, and belongs to the technical field of wound repair dressing. The in-situ collagen hydrogel dressing prepared from 1-3-year-old giant salamander skin through pretreatment, decolorization, removal of impure protein, degreasing and other steps has a natural three-dimensional honeycomb porous structure. The water vapor transmission rate of the dressing is 2000-2500 g / (m 2 24h), the dressing has excellent water retention and dynamic fitting properties, can realize efficient drug loading and slow release, has a significant bacteriostatic effect on escherichia coli and staphylococcus aureus, and has no cytotoxicity. Animal experiments show that the dressing can effectively reduce wound inflammation, promote granulation tissue growth and re-epithelialization, and significantly improve the healing speed and quality of deep Ⅱ degree burn wounds and other wounds. The application has simple process, good biocompatibility, good mechanical and functional adaptability, and is suitable for the repair and nursing of infected wounds and burn wounds, and has good clinical transformation and application prospect.

The application relates to the field of biomaterials, and particularly relates to application of a giant salamander mucus extract hydrolysate in preparation of an anti-dentin sensitivity agent. The anti-dentin sensitivity agent comprises a hydrolysate retaining the biological activity of SSAD, the hydrolysate being a product of reduction of the giant salamander mucus extract by an acid solution with a pH value of 1-5, and the product comprising five active proteins, namely MHC class I molecule alpha chain, homeobox protein Hox-D13, abnormal heavy chain 3 of axonemal dynein, ATP synthase protein and cytochrome b; the particle size of the giant salamander mucus extract is 1-50 mu m; and the pH value of the anti-dentin sensitivity agent is 5.5-7.5. The giant salamander mucus extract is treated by the acid solution to obtain the hydrolysate containing the multiple active proteins, the hydrolysate can effectively promote deep closure of dentin tubules, and has long-term stable biocompatibility; the pH value is controlled to ensure that the biological activity is completely retained, and the curative effect of the anti-dentin sensitivity agent is remarkably improved.