93 results about "Giant salamander" patented technology

The invention discloses a preparation method and permeation promotion application of a high-purity sponge bone needle, and belongs to the technical field of sponge bone needles. Comprising the following steps: cutting a natural sponge into pieces, soaking in deionized water, carrying out ultrasonic oscillation, filtering, and cleaning to obtain a pretreated sponge; carrying out enzymolysis on the pretreated sponge with alkaline protease to obtain a primary zymolyte; and carrying out enzymolysis with ginger protease to obtain a secondary enzymolysis product, and carrying out vacuum freeze drying to obtain the high-purity sponge spicule. The natural sponge is subjected to enzymolysis treatment through alkaline protease and ginger protease, and the high-purity sponge spicule is obtained; wheat oligopeptide is grafted on the surface of a sponge spicule, and giant salamander skin collagen, a pomegranate seed extract, a lonicerae flos extract, a centella asiatica extract, an early celery extract, an iris tectorum extract, an oyster shell extract and calcium chloride mixed treatment materials are loaded, so that the repairing effect on skin inflammation acne is improved.

The invention relates to the field of biological medicine, and provides a preparation method of a fermentation product containing giant salamander peptide, which comprises the steps of stepped enzymolysis pretreatment, staged inoculated fermentation, synergistic enzymolysis and molecular weight grading purification. The method comprises the following steps: firstly, carrying out staged enzymolysis on giant salamander tissues by utilizing pepsin, trypsin and flavourzyme to obtain a primary peptide solution; then, bacillus subtilis, lactobacillus plantarum and bifidobacterium are adopted for directional fermentation, and flora metabolism is optimized through dissolved oxygen control and pH automatic regulation. Then, compound protease is added for secondary enzymolysis, so that the yield of the small molecule peptide is increased. And finally, carrying out ultrafiltration and nanofiltration graded purification, vacuum concentration, freeze drying and gamma-ray sterilization to obtain the micromolecular giant salamander peptide. The molecular weight of the obtained product is mainly distributed within 0.5-5 kDa (lt), and the proportion of 3 kDa is larger than or equal to 80%), the DPPH free radical scavenging rate is larger than or equal to 85%, the ABTS < + > scavenging rate is larger than or equal to 80%, and the obvious anti-oxidation and auxiliary liver protection effects are achieved.

The invention relates to the technical field of preparation methods of giant salamander peptide and eucommia ulmoides male flower tablets, in particular to a preparation method of giant salamander peptide and eucommia ulmoides male flower tablets. The giant salamander peptide and eucommia ulmoides male flower tablets comprise the following raw materials in parts by weight: 30-40 parts of giant salamander peptide powder, 20-25 parts of eucommia ulmoides male flowers, 10-15 parts of rape pollen, 10-15 parts of sea cucumber intestine peptide powder, 10-12 parts of a filler, 3-5 parts of an adhesive and 1-3 parts of a lubricant. According to the invention, the eucommia male flower contains geniposidic acid, chlorogenic acid, aucubin lignin, iridoid and phenylpropanoid natural active nutrient substances, flavonoid compounds, polysaccharide and other bioactive substances in the rape pollen, polysaccharide and peptide components in the sea cucumber intestine peptide powder, and the like. The andrias davidianus peptide powder is added into the andrias davidianus peptide powder, the andrias davidianus peptide powder cooperates with antioxidant peptide and immunomodulatory peptide in the andrias davidianus peptide powder to jointly form a rich bioactive substance library, and the components regulate the physiological functions of the human body from different angles, provide comprehensive nutrition support for the body and improve the fatigue resistance of the human body.

The invention relates to the technical field of giant salamander by-product quality evaluation, and discloses a giant salamander by-product source functional factor quality evaluation system and method, and the method comprises the steps: obtaining a giant salamander by-product sample, carrying out the pretreatment, and extracting a functional factor to obtain a to-be-detected sample; identifying and quantitatively determining specific markers based on biochemical analysis and molecular detection means to generate a marker data set; inputting the marker data into a multi-modal feature fusion module, extracting activity and stability features, and performing time sequence dynamic modeling; activity and stability independent evaluation branches are set, and corresponding score values are generated; dividing functional domains according to functional factor categories, constructing an evaluation curve based on historical data, substituting a target sample into each functional domain and an adjacent functional domain to generate functional domain evaluation results, and performing weighted fusion; and finally, carrying out weighted integration on the activity score, the stability score and the comprehensive evaluation value, and determining a final quality evaluation result.

The invention discloses a giant salamander larva breeding device in the field of giant salamander breeding, and the device comprises a breeding frame which is of a multi-layer structure; the breeding boxes are arranged on the interlayer platforms of the breeding frame, the breeding boxes are divided into a plurality of areas through isolation structures, and the isolation structures have a water passing function; the water circulation system is arranged on the breeding frame and is used for supplying water to the breeding box on each interlayer platform and recycling overflow water; the giant salamander larva breeding device has the advantages that by arranging the breeding box with the isolation structure and the water circulation system, food protection of giant salamander larvae in the breeding process can be greatly avoided, the survival rate of the giant salamander larvae is increased, the time of manual care is shortened, and more materials can be provided for releasing the giant salamander larvae outdoors.

The invention relates to the technical field of giant salamander peptide separation and purification, in particular to a giant salamander peptide extraction and separation and purification method and system.The method comprises the steps that healthy giant salamanders and giant salamander processing equipment are determined, giant salamander processing by-products are obtained, the giant salamander processing by-products are pretreated, to-be-processed by-products are obtained, the to-be-processed by-products are degreased and deodorized, and the giant salamander peptide is obtained. Preparing giant salamander protein dry powder, preparing giant salamander crude peptide liquid, obtaining a target peptide fragment, finely separating the target peptide fragment to obtain target active peptide distillate, freeze-drying the target active peptide distillate to obtain high-purity giant salamander peptide powder, and storing the molecular weight, amino acid sequence and activity verification parameters of the peptide fragment. And extracting, separating and purifying the giant salamander peptide based on the optimized storage database and the giant salamander grease. The extraction efficiency and the separation and purification efficiency of the giant salamander peptide can be improved.

The invention relates to the technical field of automatic identification of animal behaviors, in particular to an automatic identification method for pulmonary respiration behaviors of giant salamanders, and the method comprises the steps: obtaining an initial video image of a target region; preprocessing the initial video image to obtain a standard video image; performing feature enhancement on the standard video image to obtain an enhanced feature image; performing multi-scale respiratory behavior feature extraction on the enhanced feature image to obtain depth feature maps of different scales; performing multi-level feature aggregation on the depth feature maps of different scales, and generating an aggregated feature map; and generating an identification result based on the aggregated feature map. According to the method, the problems that the omission ratio of manual monitoring of the breathing behavior in a night low-contrast scene is high and long-term efficient and objective monitoring is difficult to realize due to night walking and cave habits of the giant salamander are solved.

The invention relates to the technical field of skin care products, and particularly discloses a composition based on anti-fatigue giant salamander peptide and a preparation method and application thereof. The andrias davidianus peptide mask is prepared from the following raw materials: 3 to 5 parts of a humectant, 3 to 8 parts of a thickening agent, 4 to 5 parts of an emulsifier, 0.1 to 0.3 part of an antibacterial preservative, 0.03 to 0.06 part of andrias davidianus peptide, 0.05 to 0.1 part of sodium hyaluronate, 0.1 to 0.2 part of carbomer, 5 to 8 parts of caprylic / capric triglyceride, 60 to 70 parts of deionized water, 1.2 to 1.5 parts of modified beta-cyclodextrin and 0.3 to 0.1 part of a modified chitosan compound. When the anti-fatigue giant salamander peptide-based composition is applied, the giant salamander peptide contained in the anti-fatigue giant salamander peptide-based composition is not easily damaged due to external environment change, and can stay on the skin surface layer for a long time.

The invention discloses a giant salamander mucus extraction device, and relates to the technical field of giant salamander mucus extraction.The giant salamander mucus extraction device is characterized in that a plurality of rolling shafts are arranged in an extraction box at equal intervals in the length direction, the side end of the extraction box is fixedly connected with an air conditioner, the air conditioner communicates with an inner cavity of the extraction box through an air conveying pipe, and the two ends of each rolling shaft are rotationally connected with the inner wall of the extraction box; the material bearing box is fixedly connected with the side end of the extraction box and communicates with an inner cavity of the air conveying pipe through a connecting pipe, the side ends of the bottoms of the sliding tables are fixedly connected with a controller, the tops of the sliding tables are slidably connected with guide rails, the side ends of the controllers are slidably connected with scraping assemblies, and the bottom ends of the controllers are fixedly connected with sensors. Diethyl ether in a material bearing box is introduced into the extraction box by an air conditioner through an air conveying pipe and a connecting pipe, the andrias davidianus is anesthetized by the diethyl ether, and when mucus on the skin of the andrias davidianus is scraped, the phenomenon that the skin of the andrias davidianus is damaged by a scraping plate due to resistance of the andrias davidianus is avoided.

The invention relates to giant salamander peptide and a preparation method and application thereof, the giant salamander peptide can be obtained by hydrolyzing collagen by neutral protease, pepsin, alkaline protease or trypsin, the average molecular weight is 200-5000 Da, and the polydispersity index is 1.2-1.5. The preparation method comprises the following steps: raw material treatment: obtaining giant salamander skin tissues, degreasing and decolorizing the giant salamander skin tissues with ethanol and deionized water, and air-drying; preliminary enzymolysis and separation: performing preliminary hydrolysis by using pepsin, adjusting the pH value, performing salting-out precipitation, and performing centrifugal collection; purification and preparation of a collagen solution: dialyzing to remove impurities and freeze-drying to prepare collagen, and dissolving to form the collagen solution; enzymolysis, separation and purification: adding protease for hydrolysis, centrifuging after enzyme deactivation, filtering, carrying out molecular weight grading purification, and freeze-drying to obtain the giant salamander peptide. The invention provides the method for efficiently extracting the giant salamander collagen peptide, the purity and uniformity of the giant salamander peptide are improved, the dispersity index is reduced, and the method is suitable for the fields of functional health care products, medicines and cosmetics.

The invention discloses an instant giant salamander collagen freeze-dried ball and a preparation method thereof. The freeze-dried ball comprises 10-20 parts of giant salamander hydrolyzed collagen, 10-15 parts of mannitol and 30-50 parts of sodium polyglutamate. The freeze-dried ball can be combined with the giant salamander hydrolyzed collagen through electrostatic interaction or hydrogen-bond interaction by utilizing the compounding effect of the sodium polyglutamate and the mannitol, so that the giant salamander hydrolyzed collagen is prevented from being gathered, the giant salamander hydrolyzed collagen can be helped to be better dispersed in water, and meanwhile, the sodium polyglutamate has good moisture retention, water absorption and biocompatibility, so that the freeze-dried ball can be used for preparing the giant salamander hydrolyzed collagen. The mannitol can form a stable supporting structure in the freeze-drying process, so that the giant salamander hydrolyzed collagen is prevented from shrinking or deforming in the freezing and drying processes, the activity of the giant salamander hydrolyzed collagen is protected, and the mannitol can be matched with the sodium polyglutamate in the dissolving process, so that the giant salamander hydrolyzed collagen can be dissolved in the freeze-drying process; the giant salamander hydrolyzed collagen is quickly released, and the dissolving speed is increased.

The utility model discloses giant salamander amino acid nutrient solution filling and sealing equipment, which belongs to the technical field of filling equipment and comprises a conveying seat, a plurality of conveying grooves are formed in the top surface of the conveying seat, conveying assemblies are arranged in the conveying grooves, sealing bottles are placed on the top surfaces of the conveying assemblies, and the front side of the conveying seat is connected with a cylinder body of a lifting cylinder. Piston rods of the two lifting air cylinders are vertically and upwards connected with a liquid filling mechanism, the rear side of the conveying base is connected with column bodies of hollow columns, a cover sealing mechanism is arranged between the two hollow columns, and limiting assemblies are arranged behind the liquid filling mechanism and the cover sealing mechanism. According to the device, a plurality of sealing bottles are simultaneously conveyed to the position below the liquid filling mechanism for liquid filling through the conveying seat, then the sealing bottles filled with liquid are uniformly conveyed to the position below the capping mechanism for capping and sealing, manual assistance is not needed in the whole process, and the device has the advantages of being compact in structure and high in efficiency and is suitable for large-scale flow line production.

The invention relates to the technical field of grinding extraction, and provides a preparation method and application of a giant salamander mucus active extract based on liquid nitrogen grinding, and the preparation method of the giant salamander mucus active extract comprises the following steps: electrically stimulating the body surface of a giant salamander, and collecting giant salamander mucus; the collected giant salamander mucus is pre-cooled and then freeze-dried at the temperature of-80 DEG C, and a mucus freeze-dried substance is obtained; carrying out liquid nitrogen grinding on the mucus freeze-dried substance, and continuously obtaining grinding images within a preset time at the same time; grinding parameters are obtained based on the grinding image; mixing and stirring the ground powder and water to obtain an extract mixed solution; centrifuging and filtering the extract mixed solution to obtain extract filtrate; and pre-cooling the extract filtrate at-80 DEG C, and freeze-drying to obtain the giant salamander mucus active extract. The method improves the active content of the giant salamander mucus active extract.

1. Name of the design product: bath thermometer (giant salamander). 2. Use of the design product: thermometer for measuring water temperature when children take a bath, and also toy. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The invention belongs to the field of aquaculture, and particularly relates to a breeding method for improving growth and development of giant salamanders and improving intestinal beneficial microbial communities. The invention provides a comprehensive method which can obviously promote the growth and development speed of giant salamanders, improve the utilization efficiency of feed, directionally optimize the intestinal beneficial flora structure, enhance the intestinal health and immune function and finally realize healthy and efficient ecological breeding of giant salamanders.

The present invention discloses a rosa roxburghii tratt fruit peptide drink and a preparation method thereof, and relates to the technical field of nutritional health drink processing, and the rosa roxburghii tratt fruit peptide drink consists of the following raw materials in parts by mass: 100 parts of rosa roxburghii tratt fruit juice, 1.5-2.5 parts of a lucid ganoderma extract, 0.8-1.2 parts of a radix astragali seu hedysari extract, 4.5-7.5 parts of a coix seed extract, 8-12 parts of giant salamander peptide, 4.0-6.5 parts of locust bean gum, 0.03-0.05 part of stevioside and 60-100 parts of water. According to the rosa roxburghii tratt peptide beverage and the preparation method thereof, the rosa roxburghii tratt is scientifically compounded with various medicinal and edible substances and nutritional ingredients such as the lucid ganoderma, the astragalus membranaceus, the coix seeds and the giant salamander peptide for the first time, and a nutrition complementary composite system is formed. The compounding of lucid ganoderma and roxburgh rose has a scientific basis supported by literatures.

This application belongs to the field of biotechnology, specifically relating to giant salamander skin collagen peptides, their preparation methods, and applications. This application provides a method for preparing giant salamander skin collagen peptides. The method first pre-treats the fish skin, then performs ultrasonic-assisted extraction under acidic conditions to effectively promote collagen dissolution. The extract undergoes flocculation to remove impurities, significantly reducing interference from other proteins. The enzymatic hydrolysis stage employs a stepwise enzymatic hydrolysis strategy: first, a combination of alkaline protease and another specific protease is used for hydrolysis, sequentially cleaving the complex structure of giant salamander skin collagen to improve initial hydrolysis efficiency and generate specific intermediate peptides; subsequently, a flavor protease is added for further deep hydrolysis to achieve complete hydrolysis. This method has high enzymatic hydrolysis efficiency, controllable hydrolysis degree, concentrated molecular weight distribution of the obtained collagen peptides, high yield of the target product, and stable process.

The utility model relates to the technical field of breeding equipment, and discloses novel intelligent andrias davidianus imitated ecological breeding equipment, an automatic feeding assembly is installed at the upper end of a box body of a breeding box, and water quality monitoring assemblies are installed on the two sides, right opposite to a circulating guide plate, of a plate frame in the box body of the breeding box; a sewage treatment assembly is installed on one side of a box body of the breeding box in a communicating mode, and an aeration assembly is installed on the other side of the box body of the breeding box in a communicating mode and staggered from the circulating flow guide plate. According to the giant salamander breeding device, the breeding box is used as a breeding carrier of giant salamanders, the matched automatic feeding assembly is used for automatically feeding the giant salamanders, sewage in water is regularly extracted and filtered by means of circulating extraction and filtration of the sewage treatment assembly, and the aeration assembly and the circulating guide plate are combined, so that the giant salamanders are automatically fed into the breeding box. On one hand, fresh air is circulated and aerated into water, sufficient survival oxygen is provided for giant salamanders, on the other hand, water flow is pushed to circularly and dynamically flow, and a comfortable ecological environment is provided for giant salamanders.

The application discloses a kind of embroidery needle method for showing the special body shape longitudinal fish scale of Wuchang fish, it is related to embroidery technical field, specifically a kind of embroidery needle method for showing the special body shape longitudinal fish scale of Wuchang fish, its steps include: first, the pattern designed according to the body shape of Wuchang fish is embroidered on the cloth, and the pattern is embroidered around using silver wire and colored silk thread, from the tail of fish, silver wire is wound into the ring with a diameter of 1-2mm, and the silk thread is fixed, then, it is repeated longitudinally to the direction of fish head, and is overlapped with the previous ring, forming the circular embroidery mark arranged longitudinally, simulating the longitudinal texture of fish scale, and the three-dimensional sense of decorative needle method is improved by embroidering from the tail of fish to the direction of fish head. The method can accurately show the characteristics of the special body shape and longitudinal fish scale of Wuchang fish, and is suitable for embroidery art, clothing and home accessories manufacturing.

This invention discloses a compound composition of giant salamander peptide extract and its application in sleep-promoting products. The giant salamander meat is hydrolyzed with papain at 45-55°C, followed by hydrolysis with a complex protease, and the enzymes are inactivated after hydrolysis. The mixture is then filtered through a ceramic membrane and adsorbed using activated carbon to obtain the giant salamander peptide extract. The giant salamander peptide extract is compounded with casein peptide, tea theanine, daylily extract, poria cocos extract, jujube seed extract, lily powder, and γ-aminobutyric acid (GABA) to obtain a compound composition. Applying this compound composition to sleep-promoting products can enhance their sleep-aiding effects.

The present invention belongs to the technical fields of cosmetics and biomedicine, and particularly relates to a fifteen - peptide based on giant salamander hydrolyzed collagen and its application. The amino acid sequence of the fifteen - peptide of the present invention is Gly - Ala - Thr - Gly - Pro - Thr - Gly - Pro - Thr - Gly - Pro - Ala - Gly - Pro - Lys. The fifteen - peptide shows a significant promoting effect on the content of CollagenⅢ in fibroblasts induced by UVA radiation. At the same time, it has a good inhibitory effect on the activity of tyrosinase, and has a strong scavenging effect on DPPH free radicals and ABTS+ free radicals, indicating that the fifteen - peptide has good skin firming, anti - aging and whitening effects and can be used for the preparation of cosmetics.

This invention discloses a method for detecting the freshness of giant salamander meat based on color constancy and integrated intelligent vision, belonging to the fields of food safety and computer vision technology. The method includes: acquiring RAW images of the meat sample and an indicator film and resolving them into linear radiosity tensors; constructing a color correction model using a latent diffusion local redraw network and a conditional control network; synthesizing a structure-constrained virtual reference color card in a masked region; extracting ambient light source features and performing adaptive white balance compensation to output a standard color image; inputting this image into a deep network optimized by ArcFace to extract a high-dimensional feature vector; and finally inputting this vector into an XGBoost model to fit a nonlinear decision boundary and outputting a freshness rating. This invention effectively reduces color shift interference from complex light sources, improves the recognition accuracy of samples in the critical period of color change, and provides a highly robust solution for non-destructive monitoring of giant salamander meat freshness in actual market environments.

The present application provides a viscosity controllable chitosan-giant salamander skin secretion hydrogel and a preparation method and application thereof, and belongs to the technical field of biomedical materials.The giant salamander skin secretion lyophilized powder is mixed and stirred with ascorbic acid solution respectively to obtain a giant salamander skin secretion solution and a chitosan solution; the giant salamander skin secretion solution and the chitosan solution are mixed in a mass ratio of 6-18:1 to obtain the viscosity controllable chitosan-giant salamander skin secretion hydrogel. The viscosity of the hydrogel can be effectively controlled, and the rheological properties of the hydrogel can be controlled by adjusting the mass ratio of the giant salamander skin secretion solution and the chitosan solution. The chitosan-giant salamander skin secretion hydrogel prepared by the present application has good physical properties, antibacterial hemostatic performance and biocompatibility, and can be widely used in the field of wound healing.

The present invention provides a nano-antibody targeting giant salamander iridescent virus and its preparation method and application; the invention aims to provide a nano-antibody targeting giant salamander iridescent virus with low expression cost, low difficulty, stable source, and the ability to effectively bind to giant salamander iridescent virus and specifically realize immune analysis of giant salamander iridescent virus; the amino acid sequence of the nano-antibody is shown in SEQ ID NO.1-6 and belongs to the field of biological science and technology.

A segmented extraction device for a nutrient solution used in giant salamander protein production, which belongs to the technical field of biological extraction, and comprises a casing assembly (1), a

The invention relates to the technical field of animal grease extraction, in particular to an extraction process of giant salamander grease rich in EPA and DHA, which comprises the following steps: step 1, raw material refining pretreatment: selecting tail adipose tissues of cultured giant salamanders' second filial generation 'and offspring thereof meeting the GB2733 standard, and carrying complete inspection and quarantine proof, and legitimacy and safety of the raw materials are ensured. According to the method disclosed by the invention, the total extraction rate of the giant salamander tail grease oil is greater than or equal to 76% through a synergistic process of low-temperature composite enzymolysis activation, gradient temperature control vacuum decoction and secondary short-time oil extraction of residues, is improved by 13.18% compared with 68.61% of an original vacuum decoction scheme, and is far higher than 3%-5% of a conventional improvement in the field; and the residual oil rate is reduced from 8-10% of the original scheme to less than or equal to 3%, and the reduction amplitude reaches 77.5%, so that the full utilization of oil resources is realized, and the economic benefits of industrial production are greatly improved.

The invention provides a preparation method of giant salamander peptide, and belongs to the technical field of bioactive peptide preparation. The quality of the giant salamander peptide is greatly improved through low-temperature stress treatment before slaughtering, variable-pressure boiling, beta-cyclodextrin and sequential addition of neutral protease, papain, bromelain and the like, the finally obtained giant salamander peptide is high in small molecule peptide proportion and low in bitter taste, and the biological activity is remarkably superior to that of a product prepared by a traditional method; the method is very suitable for developing high-end functional food and health care products.

The feeding device comprises a U-shaped clamping base, a feed cylinder, a stirring device, a feed throwing disc and a feed throwing device, adjusting supporting rods are vertically and symmetrically arranged on the U-shaped clamping base, the feed cylinder is arranged between the upper ends of the supporting rods, and a discharge pipe is arranged on the side face of the lower end of the feed cylinder in an inclined and downward mode. The stirring device comprises a first motor and stirring blades, the stirring blades are arranged on an output shaft of the first motor, the throwing disc is arranged at the lower end of the discharging pipe and communicated with the discharging pipe, the throwing device comprises a second motor and a centrifugal throwing disc, and an output shaft of the second motor downwards penetrates through the center of the throwing disc. The device can be clamped on a breeding box and is convenient to use, the height position of the feed throwing disc can be adjusted according to requirements by adjusting the supporting rod, the stirring device can stir and mix feed in the feed cylinder, the feed throwing device throws the feed, the work efficiency of food throwing is improved, and the device is simple in structure, convenient to use and high in practicability. The practicability is higher.

The invention belongs to the technical field of refrigeration, and particularly relates to refrigeration equipment for andrias davidianus freezing pretreatment, which comprises a refrigeration equipment main body, a refrigeration box is fixed on the upper side of the refrigeration equipment main body, and symmetrical sealing doors are rotatably connected to the front end of the refrigeration box; in the process that giant salamander freeze-dried powder is placed in freeze-dried powder containing discs for refrigeration, two rotating rods can be driven to rotate through a transmission mechanism, the two rotating rods drive a rotating disc and a first transverse rod to rotate, the first transverse rod drives the multiple giant salamander containing discs to rotate, and therefore cold air in the refrigeration box can be continuously and evenly mixed and stirred; the contained giant salamanders are freeze-dried and cooled uniformly; in the process of freeze-drying and refrigerating the giant salamanders through the giant salamander containing plate, the contained giant salamander freeze-dried bodies can be turned over through cooperation of a moving mechanism, a connecting block, a second transverse rod and a uniform turning rod, and therefore the situation that the giant salamander freeze-dried bodies are damaged due to caking in the long-time refrigerating process is avoided.

The present application relates to the technical field of bioactive peptides, and specifically discloses a preparation method and application of giant salamander hydrolyzed collagen active peptide. The amino acid sequence of the giant salamander hydrolyzed collagen active peptide is SPPRARV. The single polypeptide in giant salamander peptide powder is separated and identified by combining gel chromatography column and preparative chromatography for the first time, and the giant salamander hydrolyzed collagen active peptide with the amino acid sequence of SPPRARV is successfully obtained. The polypeptide is modified by using molecular docking technology, and the obtained modified peptide has good transdermal ability and cell repair ability, can promote cell scratch repair and cell proliferation, promote the increase of type I collagen content, and is suitable for skin repair and anti-aging medical and beauty products or skin damage repair drugs.