107 results about "Andrias" patented technology

The invention relates to an artificial culturing method of a wild animal, in particular to a high-density culturing method of andrias davianus (giant salamanders). The high-density culturing method of the andrias davianus (giant salamanders) is characterized by comprising the following steps of: building 5-10 indoor stepped culture ponds on a sloping field with an angle of 5-10 degrees, wherein water used for circulating over flows is adopted in each culture pond; strictly filtering and purifying water quality, and keeping the temperature of the water at optimal growth temperature between 19 DEG C and 22 DEG C; and culturing the andrias davianus (giant salamanders) in a pond separation and classification way. The invention can provide optimal growth conditions for the culture of the andrias davianus (giant salamanders) and also save a large amount of energy, enhances the culture density and the survival rate, reduces the cost and increases the culture benefits.

The invention relates to a preparation method of mucus protein peptide of andrias davidianus. The preparation method comprises the steps that 1, mucus of the andrias davidianus is collected through an electric shock stimulus method, and freeze-dried to obtain freeze-dried mucus powder; 2, the obtained freeze-dried mucus powder is added to distilled water with the mass of 50-120 times, then alkaline protease which is 10 percent-30 percent of the mass of the freeze-dried mucus powder is added, pH is adjusted to 9-9.5, enzymolysis is carried out for 1-3 h under the temperature condition of 40 DEG C-60 DEG C, and then mixed liquid is boiled for 1-2 min and cooled to 19 DEG C-25 DEG C; 3, the mixed liquid treated in the step 2 is centrifuged for 10-20 min under the condition of 3,000-4,500 r/min, clear liquid is taken, the pH value of the clear liquid is adjusted to 4-5, under the temperature condition of 40 DEG C-45 DEG C, activated carbon of one percent to two percent is added, stirring is carried out for 45-60 min for decolorizing and deordorizing, and then under the condition of 3,000-4,500 r/min, centrifugation is carried out for 10-15 min, and filter liquor is obtained through a filter film of 0.45 micrometer; 4, the filter liquor treated in the step 3 is concentrated to 1/8 -1/7 of an original sample under the temperature condition of 30 DEG C-60 DEG C in a rotary evaporation mode, concentrated liquor is sealed with a plastic bag, sterilization is carried out for 1-5 min under the conditions that temperature ranges from 19 DEG C to 25 DEG C and pressure ranges from 150 MPa to 250 MPa, and vacuum freeze-drying and smashing are carried out in clear environment to obtain mucus protein peptide powder.

The invention discloses an andrias davidianus culture pond having a water circulation function. The andrias davidianus culture pond comprises a culture pond, a biochemical pond, a disinfection tank and a water pump. An aerator is installed on one side of the culture pond, a filter screen is installed above the aerator, a first water valve is installed on the outer side of the filter screen and connected with a water inlet pipe, a controller is installed on the outer wall of the other side of the culture pond, a second water valve is arranged below the controller, a metal net is disposed at the bottom of the culture pond on the inner side of the second water valve, the outer side of the second water valve is connected with a drain pipe, a flow meter is installed on the drain pipe, and the other end of the drain pipe is connected with the biochemical pond. A slope sedimentation tank is arranged on one side of the biochemical pond, and the disinfection tank is disposed on one side of the slope sedimentation tank. The andrias davidianus culture pond has the advantages that water replacing time and water replacing quantity can be set, the water discharge time and the water replacing quantity can be controlled automatically, discharged sewage is purified, environmental pollution is avoided, the purified water can be reutilized, the water utilization rate is greatly improved, and water resource waste is effectively avoided.

The invention relates to the technical field of animal breeding, particularly to a traditional Chinese medicine for promoting andrias davidianus male parent fish gonad maturation, achieving synchronous development maturation of andrias davidianus male parent fish gonad and andrias davidianus female parent fish gonad, and improving an andrias davidianus reproduction rate, and a preparation method thereof. The traditional Chinese medicine is prepared from the following traditional Chinese medicine raw materials, by weight: 15-25 parts of tuber fleeceflower root, 12-20 parts of eucommia bark, 5-12 parts of siberian solomonseal rhizome, 8-17 parts of medlar, 42-50 parts of angelica archang lica, and 0.5-1 part of carp pituitary, wherein the aged tuber fleeceflower root, the defatted carp pituitary, the eucommia bark, the siberian solomonseal rhizome, the medlar and the angelica archang lica are respectively crushed according to the weight part ratio, and then are uniformly mixed to prepare into capsules. According to the present invention, the traditional Chinese medicine is prepared by adopting the traditional Chinese medicine raw materials, no toxic-side effect is generated, synchronous development maturation of andrias davidianus male parent fish gonad and andrias davidianus female parent fish gonad is effectively promoted, an andrias davidianus reproduction rate is increased, pollution on water environment for andrias davidianus growth can not be generated, a sperm amount produced by the andrias davidianus male parent fish can be effectively promoted to be increased by 35-80%, sperm motility is increased to 35-70%, and an artificial fertilization success rate is increased to 70%.

A use of mucus of andrias davidianus Blanchard is disclosed. An extract product of the mucus is used for wound healing medicines and wound healing medical instruments. The extract product is used for traumatic bleeding, burn bleeding, scald bleeding and surgical operation bleeding. Pharmacological tests prove that the extract product of the mucus has a good wound healing function.

The invention relates to an andrias davidianus breeding method. According to the breeding requirements, the method includes the steps of construction of a farm, putting of fry, feeding and management and environment creating. A good natural environment of an andrias davidianus artificial breeding pond is created, zero breakthrough of andrias davidianus artificial breeding is achieved, and it is guaranteed that the survival rate of andrias davidianus is 80-90%. In order to change difficulties in the andrias davidianus industry, normal growth of andrias davidianus in different growth periods is guaranteed by discharging water and adding water, artificial requirements are reduced, and the method has the advantages of being convenient to use, high in professionality, beneficial to saving cost, capable of guaranteeing the survival rate and the like, and the growth state is good.

The invention discloses an andrias male and female natural pairing imitative ecological efficient breeding method. The method comprises the steps that male and female andrias are raised in a mixed mode, wherein the young male and female andrias is raised in a mixed mode; trial pairing is conducted on breeding male and female andrias, wherein when getting close to sexual maturity, the andrias is placed into a trial paring pool, in a breeding season, namely between August and September, the secondary sex characteristic becomes obvious, the pairing succeeds, the andrias is selected out and placed into a breeding pool, and the trial paring pool and the breeding pool are constructed and constituted by multiple river channels and caves; breeding is conducted in the breeding pool, wherein the andrias which is successful in pairing is placed into the breeding pool which is provided with only two caves, female andrias needs to be isolated after laying eggs, and male andrias is not disturbed to hatch; young andrias is raised, wherein the young andrias is not fed from the shell-emerging time to the 30th day, 30 days later, the young andrias is fed with tubificidae and water earthworm, one year later, the young andrias is fed with young grass carps and young carps, three years later, the andrias is sold as commodities, and seven years later, excellent offsprings are reserved for breeding; bait fishes can be casted after the bait fishes are raised for three to five days temporarily and no disease attach is found; at the earlier ovulation stage, the female andrias needs to be fed with crabs, loach and shrimps; water bodies, water channels and bait are disinfected with 0.5-3% of disinfection salt, and appliance is disinfected with potassium permanganate; when the bait fishes are casted, the bait density is increased.

The invention discloses a training facility for increasing the field viability of andrias davidianus. The training facility is characterized by comprising a mountain spring water purifying pool, andrias davidianus culturing pools, a training pool and a forage fish culturing pool, wherein the mountain spring water purifying pool, the andrias davidianus culturing pools, the training pool and the forage fish culturing pool are provided with water inlet ends and water outlet ends; the water inlet end of the mountain spring water purifying pool is connected with upstream river water, and the wateroutlets of the mountain spring water purifying pool are connected with the water inlet ends of the andrias davidianus culturing pools; the water outlet ends of the andrias davidianus culturing pools are connected with the water inlets of the training pool; the water outlet end of the training pool is connected with the water inlet end of the forage fish culturing pool; the water outlet end of theforage fish culturing pool is connected with an external drainage ditch. The invention also provides a method for training and culturing the andrias davidianus by utilizing the training facility. Through artificial training, the initiative predation capability of the andrias davidianus can be strengthened, andrias davidianus fries which can adapt to a field natural environment and have strong bodies, strong swimming ability, operation resistance and strong initiative predation capability can be cultured under an artificial condition, and a good release effect can be finally realized.

The invention discloses a giant salamander iridovirus TaqMan real-time fluorescent quantitative PCR (polymerase chain reaction) kit and application thereof. The kit comprises sterile double distilled water, a 10*Taq reaction buffer, 2.5mmol/L dNTPs (deoxyribonucleotide triphosphates) and a fluorescent quantitative reaction liquid. The kit is characterized in that the fluorescent quantitative reaction liquid contains primers and a fluorescent probe, wherein the primers are a forward primer and a reverse primer, the forward primer is 5'-GCGGTTCTCACACGCAGTC-3', the reverse primer is 5'-ACGGGAGTGACGCAGGTGT-3', and the fluorescent probe sequence is 5'-AGCCGACGGAAGGGTGTGTGAC-3'; the 5' terminal marker of the fluorescent probe is FAM, and the 3' terminal marker is ROX; 5U/mu L Taq DNA (deoxyribonucleic acid) polymerase and standard positive template pMCP are a pMD19-T vector composed of 1392 nucleotide segments containing giant salamander iridovirus MCP gene; and the vector proliferates in colibacillus E.coli DH5a. The invention also discloses application of the kit in detection of giant salamander iridovirus pathogen. The kit is quantitative and accurate, and has the advantage of high detection speed (the detection only takes 1 hour, and totally takes 2-3 hours plus the extraction and preparation of the DNA); the method is easy to implement and simple to operate; and the invention can be used for simultaneously detecting high-flux samples, and the accuracy is up to 98%.

The invention discloses fodder for breeding andrias and a processing method thereof. The fodder is prepared from the materials, in parts by weight: 76-84 parts of animal protein, 21-25 parts of sweetpotato powder, 16-20 parts of corn flour, 14-16 parts of semen sojae powder, 12-16 parts of phagostimulant, 6-8 parts of herba houttuyniae and 2-5 parts of rhizona bletillae. The prepared fodder has the balanced nutrition, can meet the growing requirements of andrias, can effectively improve the growing speed of the andrias, and the cost of the raw materials is low.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) detection kit and a detection method for giant salamander iridovirus. The LAMP detection kit for the andrias advidianus iridescent virus comprises the following ingredients: 10*Thermol Reaction buffer, BstDNA polymerase, dNTPs, outer primers F3 and B3, inner primers FIP and BIP, BEtaine, MGc12 and 1000*SYBRGreenI. The LAMP detection kit and the LAMP detection method have the characteristics of simplicity, convenience, high specificity and sensitivity, the GSIV (Giant Salamander Iridovirus) in a sample can be detected accurately within 2h by a water bath kettle or a metal bath kettle, the sick fish tissues infected by GSIV can be detected, and the cells infected by the GSIV can be detected, so that the detection kit and the detection method are very suitable for field rapid detection of GSIV.

The invention discloses a method for screening Andrias davidianus female specific fragment and detecting genetic sexuality through RAD sequencing. The method comprises: 1) scanning of Andrias davidianus male-female genome pool through RAD sequencing technology and data analysis: carrying out genomic DNA extraction, enzymatic digestion, library construction and sequencing to obtain Andrias davidianus male-female genome RAD data, and analyzing the Andrias davidianus male-female genome RAD data to obtain male-female specific candidate RAD data; 2) verification of candidate female specific fragment: selecting female specific candidate fragments, designing primers, and carrying out PCR amplification by using DNA as a template, wherein the characteristics that the fragment with the expected sizecan be amplified, and the specific band only exists in the female individual and does not exist in the male individual are used as the specific marker; and 3) sequence analysis of the female specificfragment: cloning the specific fragment into a PMD-18T vector, ligating, transfecting, detecting positive clones by using a conventional method, sending samples, and sequencing, wherein the homologyreached 96%. According to the present invention, the method has advantages of accuracy, easy performing, low cost and simple operation, and can solve the problem of Andrias davidianus gender identification in the current production.

The invention provides a method for bionic breeding of andrias davidianus, which comprises the steps: caves and artificial streams which flow through the caves are built, parent andrias davidianus which is used for bionic breeding is selected, nutrient substances are supplemented and the diversification of baits is guaranteed in a cultivation stage, the baits and the nutrient substances are feed for one time every 3-5 days, male parent andrias davidianus is naturally incubated after spawning fertilizing in a breeding season, and then egg granules which are naturally incubated are artificially incubated to germinate after being collected. Compared with implementation effects of other bionic breeding fields, the method for bionic breeding of the andrias davidianus is above 95% in fertilization rate, achieves above 95% hatching rate, is higher than a common bionic breeding method of the andrias davidianus, and has important significance for improving reproductive capacity of the andrias davidianus.

The invention relates to the technical field of facial masks, in particular to a formula of effective components of a facial mask for diminishing inflammation, removing acnes and whitening skin. Through cooperative design of dendrobium officinale, wild camellia oil, andrias davidianus mucus, bitter gourd, herba leonuri, rhizoma atractylodis macrocephalae and honey, the proportion is appropriate; effect cooperation of the effective components in the raw materials is promoted; the efficacies of moisturizing and whitening the skin, sterilizing and diminishing inflammation are improved; the repairing function of skin tissue cells is restored; the recovery rate on the skin is improved.

The invention relates to an andrias-davidianus behavior monitoring method. A device comprises a digital camera monitoring system for monitoring andrias-davidianus behaviors and a software system for andrias-davidianus behavior characteristics. The digital camera monitoring system is used for obtaining the behavior dynamic process of andrias davidianus, and the software system is used for automatic analysis and statistic of the andrias-davidianus behaviors. According to the method, real-time monitoring of the whole process of the andrias-davidianus behaviors is achieved, the growth state of andrias davidianus breeding is grasped at any moment, particularly, during andrias-davidianus breeding seasons and accident bursting, the condition of the andrias davidianus can be roundly grasped at any moment so that the management technology can be adjusted in time, the natural breeding success rate of the andrias davidianus is increased, and the safety of the bred andrias davidianus is guaranteed. In addition, the system can roundly grasp the in-and-out-hole behavior rule of the andrias davidianus, the behavioral ecological characteristics of the andrias davidianus are revealed, and a scientific basis is provided for wild andrias-davidianus population protection. As the quantity of hardware devices and the amount of labor which are required by the system are small, the cost is low, and using is simple and fast.

The invention discloses a preparation method of oxidation resisting andrias davidianus peptide Jiang-flavor wine. The preparation method is characterized by comprising the following steps of (1) thoroughly cleaning andrias davidianus meat, and performing mincing; (2) mixing andrias davidianus meat emulsion with water in the feed liquid ratio being (1 to 3) to (1 to 5), and adjusting the initial pHto 4-5; (3) adding papain, and performing hydrolyzing at 50-60 DEG C for 4-4.5h; (4) performing enzyme deactivation: after hydrolyzing, performing enzyme deactivation at 100 DEG C; (5) filtering feedliquid after enzyme deactivation, and collecting hydrolyzed filtrate; (6) performing decompressed vacuum freezing and drying on polypeptide hydrolyzate to prepare andrias davidianus polypeptide freeze-dried powder; and (7) mixing the andrias davidianus polypeptide freeze-dried powder with 38-53%vol Jiang-flavor Baijiu, performing precipitating, and performing centrifugation to prepare the andriasdavidianus peptide Jiang-flavor wine being clear and transparent. Compared with Jiang-flavor wine produced by an infusing technology, for the prepared andrias davidianus peptide Jiang-flavor wine, the content of biologically active peptide is increased by 183%, and the antioxidant activity is improved by 212%. For the peptide Jiang-flavor wine, the polypeptide content is 14.2mg/mL, the clearancerate of DPPH free radicals is 77.98%, and the clearance rate of hydroxyl free radicals is 64.5%.

The invention discloses a product for improving the immunity and a preparation method of the product. The product is prepared from the following raw materials in parts by weight: 0.1-1.0 part of andrias collagen, 0.5-2.0 parts of donkey-hide gelatin collagen and 3.0-8.0 parts of chicken embryo collagen. As active components of medicines are taken as raw materials, the utilization rate of medicines and healthcare products is increased, the healthcare function of the product is improved, and the product is relatively applicable to development of industrial modernization and massive healthcare products.

The present invention discloses an artificial feed for andrias davidianus and a preparation method thereof. The artificial feed is prepared from the following raw materials in parts by weight: 52-58 parts of fish meal, 10-18 parts of soybean cakes, 6-12 parts of wheat brans, 7-13 parts of silkworm chrysalis powder, 3-8 parts of bone powder, 3-8 parts of pollens, 3-8 parts of mixed vitamins, 3-8 parts of citric acids, 3-8 parts of methionine, 3-8 parts of tryptophan, 3-8 parts of arginine, 7-13 parts of traditional Chinese medicinal powder, 4-9 parts of fish oil, 3-8 parts of trace elements and 16-22 parts of grasshoppers. The preparation method comprises the following steps: raw material processing, traditional Chinese medicine powder preparing, grasshopper treating, mixing, drying and finished product obtaining. The prepared feed by the strict proportions of the raw materials can be suitable for the various growth stages of the andrias davidianus. Besides, compared with the traditional live baits, the feed has characteristics of being low in costs, high in nutrient, and stable in quality.

The invention discloses an imitation ecological efficient propagation method of Andrias davidianus, comprising: first, building a farm at a river section having the growth history of wild Andrias davidianus or propagation history of imitation ecological Andrias davidianus, wherein the farm has an artificial river and multiple propagating ponds serially set on the same, and each end of each propagating pond is provided with a salamander nest; putting a pair of parental salamanders in each propagating pond, with the female and male using the two nests respectively and isolated by a partition mesh; keeping the propagating ponds clean and sanitary, not icing in winter, with water temperature kept to 23 DEG C and below in summer; in early August, intramuscularly injecting LRH-a to one parentalsalamander having poor sexual gland development and maturation degrees according to its body so as to promote the development of sexual glands; in mid to late August, intramuscularly injecting LRH-a again to each parental salamander having improved but not complete development and maturation degrees of sexual glands according to the body weight so as to carry out artificial spawning induction; providing good management for laying propagation and management from egg phase to fry phase. By using the method of the invention, propagation rate of Andrias davidianus can be increased to 1:300 and above, and propagation participation rate can be increased to 70% and above.

The invention provides anti-scaring andrias skin collagen burn cream and a preparation method thereof and relates to burn cream. The preparation method comprises the steps of first taking raw materials according to the following raw material ratio: 5-15 g of andrias skin collagen, 5-15 ml of andrias oil, 35-45 ml of sesame oil, 10-20 g of beeswax, 30-40 g of sanhuang and 3-5 g of borneol, wherein the sanhuang is composed of coptis chinensis, cortex phellodendri and rhubarb according to a weight ratio of 1:1:1; smashing the sanhuang and borneol into powder to be reserved; first melting the beeswax and then adding the sesame oil and sanhuang powder, performing mixed boiling for 15-20 min at 100-105 DEG C, performing filtering, and taking liquid; when the liquid temperature is lowered to room temperature, adding the andrias oil and borneol powder, and placing the liquid in the environment at 4-10 DEG C; when the liquid temperature is reduced to 4-10 DEG C, adding the andrias skin collagen, performing stirring evenly, finally adding vaseline to obtain an ointment having a certain stickness, and obtaining the product. The burn cream can promote rapid healing of burn and scald wound surfaces, the recovery effect of the burn and scald wound surface skins is improved, and no scar remains.

The present invention relates to a method of preparing liquefied andrias davidianus protein powder by using andrias davidianus as raw materials and an enzymatic hydrolysis technology. The method prepares the andrias davidianus into andrias davidianus powder, the andrias davidianus powder is put into an extracting tank to conduct degreasing, then an appropriate amount of water and neutral protease are added the degreased andrias davidianus powder to conduct hydrolysis and filtration, then a filtrate is subjected to concentration in vacuum, and the concentrated filtrate is spray-dried to obtain the liquefied andrias davidianus protein powder. The product is soluble in water, contains more than 20 amino acids and a variety of minerals and trace elements, is comprehensive in essential amino acid compositions and rich in content, can be used in the manufactures of infant nutritional andrias davidianus paste, full nutrient, amino acid gelatin pearls, and animal and plant mixed protein powder, and can also be used to manufacture advanced nutritional seasonings.