20 results about "Mycotoxin" patented technology

This invention belongs to the field of native shrub seedling propagation technology, and discloses a system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions. The system includes a data processing platform, acquisition equipment, and control equipment. The data processing platform establishes habitat and germplasm archives corresponding to the same germplasm batch and sequentially completes germplasm dormancy treatment, staged seedling management, root culture, mycotoxin-seedling symbiosis, stress substrate integration, and planting feedback. The method generates qualified germplasm records and germination initiation records based on germplasm status and dormancy type. Seedling stage status records serve as the basis for initiating the next stage. Root culture, mycotoxin inoculation, stress pre-adaptation, and substrate transition are performed based on the habitat information of the target restoration plot. After planting, survival rate, greening time, new shoot growth, and overwintering survival rate are recorded in the habitat and germplasm archive to correct parameters for subsequent batches.

This invention discloses a test strip, reagent kit, and method for simultaneous detection of multiple mycotoxins in feed based on self-calibrated SERS lateral flow chromatography, belonging to the field of biodetection technology. The test strip includes a backing plate, a sample pad, a conjugate pad, an NC membrane, and an absorbent pad. The NC membrane is adhered to the backing plate, and the absorbent pad and the conjugate pad are respectively tightly pressed against both ends of the NC membrane. The sample pad is tightly pressed against the end of the conjugate pad furthest from the NC membrane. From the end closest to the conjugate pad to the end furthest from the conjugate pad, the NC membrane has a control line and at least one detection line sequentially arranged. This test strip based on self-calibrated SERS lateral flow chromatography has high detection efficiency, with advantages such as fast detection speed, high sensitivity, strong specificity, and simple operation. It can achieve simultaneous detection of multiple mycotoxins and is suitable for rapid detection in feed production sites and grassroots supervision.

This invention relates to the field of chromatographic detection and agricultural product quality and safety analysis technology, specifically to a system for detecting and analyzing the content of agricultural toxins based on chromatographic separation. It includes: a communication module, a signal acquisition module, a benchmark modeling module, a phase shift calculation module, a kinetic decoupling module, a quantitative analysis module, and a hardware feedback module. By synchronously acquiring spectral intensity, retention time, pump pressure transient pressure, and column oven heat flux gradient signals, a multidimensional physical space tensor is generated to establish a benchmark kinetic manifold for the pure analyte. The phase shift vector of the actual observed trajectory relative to the benchmark kinetic manifold is calculated, separating the global phase shift component from the local high-frequency response component. This invention achieves absolute quantification of the target analyte and matrix inhibition assessment, and inversely drives injection volume adjustment or knowledge base storage.

The application discloses a detection device of a mycotoxin biosensor, which comprises a button, a machine bin, a limiting plate, an elastic piece and a scanning machine. The button is electrically connected with the limiting plate through the machine bin. The limiting plate is transitionally fitted on the elastic piece. The elastic piece is gap-fitted with the scanning machine. A correction device and a pushing structure are arranged on the assisting body. The correction device and the pushing structure are mutually matched on the folding frame. When the spring bending rotary part is buckled on the 90-degree vertical angle of the limiting plate, the penetrating block is pressed on the pushing structure, the pushing structure is opened to the folding frame, the traction belt on the stacking adjusting column is pulled, the correction device is rotated to be attached to each bending rotary part of the spring, is centrally positioned between each bending rotary part, and the stereoscopic sense of the extended spring is effectively controlled.

This application relates to the field of intelligent detection technology, and in particular to a method and system for detecting mycotoxins in feed based on spectral analysis. The method includes: acquiring the near-infrared diffuse reflectance spectrum of the feed sample to be tested; constructing an original spectral vector based on the absorbance data of the near-infrared diffuse reflectance spectrum; determining the projection component of the original spectral vector on a pre-constructed qualified matrix space; subtracting the projection component from the original spectral vector to obtain a residual vector; calculating the first-order difference spectrum of the residual vector based on preset sensitivity weights; determining weighted eigenvalues ​​by local feature weighting of the first-order difference spectrum; determining the residual entropy value and the range value of the residual vector; and using the weighted eigenvalues, residual entropy value, and range value to determine the detection result of the feed sample to be tested. Through the above technical solution, more accurate detection results for mycotoxins in feed can be obtained.

The present application belongs to the technical field of feed additives, and particularly relates to a modified attapulgite for adsorbing mycotoxins and a preparation method and application thereof. The modified attapulgite is made of attapulgite and a modifier composition, and the weight ratio of the attapulgite to the modifier composition is 100:10-17. The modifier composition is made of the following raw materials in parts by weight: zeolite 25-35 parts, shrub willow powder 5-15 parts, methionine 5-10 parts, lysine 5-10 parts, yeast cell wall 10-20 parts, chitosan oligosaccharide 3-8 parts, betaine 5-15 parts, potassium sorbate 3-8 parts, and monoglyceride fatty acid ester 5-15 parts. The modified attapulgite has multiple beneficial effects such as high-efficiency and broad-spectrum adsorption of mycotoxins, improvement of animal growth performance, regulation of immune and inflammatory responses, and the like, and has significant application value and popularization prospect in the field of feed additives.

The present application relates to the technical field of microorganism, and particularly relates to a Pediococcus pentosaceus QHEK025, a composition containing the same and purposes. The Pediococcus pentosaceus is Pediococcus pentosaceus QHEK025, the preservation number is CCTCC NO: M 20252985, and the preservation is carried out in China Center for Type Culture Collection. The composition contains at least one of lactic acid dodecylbenzene and a culture of the strain. The composition is used for preparing a composition for feed fermentation. Advantages of the present application are as follows: (1) can efficiently degrade mycotoxins; (2) has probiotic properties, strong growth performance, strong acid tolerance, strong bile salt tolerance and strong osmotic pressure tolerance; (3) has good hydrophobicity and self-aggregation performance; (4) high safety.

The present application belongs to the technical field of mycotoxin biodegradation and bacillus velezensis, and particularly relates to a bacillus velezensis strain with degrading vomitoxin and multiple enzyme activities and application thereof. The strain is isolated from pig intestinal contents, and is identified by morphological identification, physiological and biochemical identification, and 16S rRNA and gyrB gene molecular identification, and is confirmed as bacillus velezensis, and is named as bacillus velezensis YK-19 (Bacillus velezensis YK-19). The strain has a degrading rate of vomitoxin (deoxynivalenol, DON) as high as 93.79%, and has multiple enzyme activities such as protease, amylase, beta-mannanase, xylanase, cellulase and pectinase, and does not have hemolytic activity, and has good biological safety. The strain also shows good acid resistance, bile salt resistance, salt resistance and high temperature resistance, and has a wide application prospect in actual production.

The application discloses an enzyme-metal-mesoporous covalent organic framework composite catalyst and application thereof in mycotoxin removal, and belongs to the technical field of biology. The composite catalyst uses nanoflower-shaped mesoporous covalent organic framework as a carrier, synthesizes metal particles in the carrier through an in-situ reduction method, fixes enzymes on the surface of the carrier through physical adsorption, and regionally co-fixes the enzymes and metal nanoparticles on the mesoporous covalent organic framework. The enzyme-metal-mesoporous covalent organic framework composite catalyst has a strong adsorption effect on mycotoxin, and simultaneously has high enzyme and metal catalytic activities. Through the strong adsorption effect and the enzyme and metal synergistic catalysis, the composite catalyst is applied to efficient removal of mycotoxin. The composite catalyst has a magnetic response and good repeated use performance. The enzyme-metal-mesoporous covalent organic framework composite catalyst provided by the application has wide applicability, a simple preparation method, is easy to be industrialized, and has a good application prospect.

This invention discloses a SNP marker for resistance to porcine vomitoxin (DON) and its application. The SNP marker is located at Chromosome 11:75541817 in the porcine genome, and its base is C or A. This SNP can enhance resistance to DON by promoting the expression of the LIG4 gene. The C / A mutation can be used as a molecular marker for breeding porcine mycotoxins. This SNP genetic marker can be used to assist in the selection of AA-type individuals with good DON resistance, which is beneficial to improving the disease resistance of the population and maximizing the economic benefits of pig farming enterprises.

Compositions, methods, systems and equipment capable of significantly reducing glyphosate, microbial, mycotoxin and other contaminant levels in various food commodities, such as, but not limited to, grains (e.g., corn, wheat, oats barley, rye, sorghum, millet, rice, etc.), nuts, seeds, and other agricultural products, are described. A contaminant treatment fluid suitable for use in reducing contaminant levels in foods may include an electrolyzed carrier fluid having a reductive potential with an oxidizer, a carbohydrate, and a sulfonic acid functional group-containing compound. Contacting food commodities with the contaminant treatment fluid reduces contaminant levels in the food commodity.

Disclosed are a nucleic acid molecule, a polypeptide having epoxy group-removing catalytic activity and use thereof. According to the invention, by means of genetic engineering, the nucleic acid molecule encoding a de-epoxidation protein is expressed in a plant, so that an epoxy group of a trichothecene mycotoxin is removed, and the toxin amount in the plant is reduced. The polypeptide of the invention is capable of catalyzing a reaction between vomitoxin and glutathione under mild conditions to remove epoxy groups to produce a glutathionylated derivative.

This invention relates to the field of microbial technology, specifically to a strain of *Pediococcus acidilactici* QHBM076, a composition containing the strain, and its uses. The *Pediococcus acidilactici* is *Pediococcus acidilactici* QHBM076, with accession number CCTCC NO: M 20252988, deposited at the China Center for Type Culture Collection. The composition comprises at least one of *Pediococcus acidilactici* and a culture of the strain. The use of the composition in the preparation of compositions for feed fermentation is also discussed. The advantages of this invention are: (1) it can efficiently degrade mycotoxins; (2) it possesses probiotic properties, strong growth performance, and strong resistance to acid, bile salts, and osmotic pressure; (3) it has good hydrophobicity and self-aggregating properties; and (4) it has high safety.

The application provides a kind of dairy cow feed for improving dairy cow production performance, comprising the following components: whole plant corn silage 48-60%, rubbing coarse feed 5-12%, energy feed 8-16%, protein feed 10-18%, byproduct fiber feed 3-8%, fat supplement 1-4%, mineral supplement 2-5%, rumen buffer component 0.8-2.0%, enzyme and bacteria compound premix 0.15-0.60% and mycotoxin control component 0.05-0.30%; the whole plant corn silage is the whole plant corn silage after lactic acid bacteria fermentation treatment; the energy feed includes one or more of pressed corn, corn meal and high-moisture corn; the mineral supplement includes stone powder, calcium hydrogen phosphate, salt, trace element premix and vitamin premix; the application solves the problems of poor stability, low utilization rate, high feed cost and insufficient production performance improvement of existing high silage ration.

This invention relates to the field of mycotoxin adsorbent preparation technology. More specifically, it relates to a yeast-loaded modified montmorillonite, its preparation method, and its application. The preparation method of the yeast-loaded modified montmorillonite includes the following steps: S1, converting calcium-based montmorillonite into sodium-based montmorillonite via a sodium-modification method, followed by thermal activation treatment; S2, mixing and grinding the thermally activated sodium-based montmorillonite with a carbon precursor, and calcining under an inert atmosphere to obtain carbon-based montmorillonite; S3, adsorbing and coupling the carbon-based montmorillonite with activated yeast to obtain yeast-loaded modified montmorillonite. This invention utilizes the organic groups modified on the surface of the modified montmorillonite to form a specific interaction with yeast, achieving directional and stable loading of yeast, enabling it to stably perform its metabolic function during mycotoxin removal.

The application discloses a non-target screening method for mycotoxins in food. For food samples, a non-target data acquisition platform of ultra-high performance liquid chromatography-high resolution mass spectrometry is used to construct a mycotoxin mass spectrum feature extraction and non-target screening method, and structure identification is combined with retention time, mass spectrum fragmentation rules and multi-source databases. The application has obvious advantages in the screening of mycotoxins in complex food matrix, and has the advantages of high analysis efficiency and high accuracy.

The present application relates to the technical field of microorganism, in particular to a strain of Enterococcus hirae QHBMA068, a composition containing the same and use thereof.The Enterococcus hirae is Enterococcus hirae QHBMA068, the preservation number of which is CCTCC NO: M 20252987, and it is preserved in China Center for Type Culture Collection.The composition contains at least one of Pediococcus acidilactici and a culture of the strain.The composition is used for preparing a composition for feed fermentation.The present application has the following advantages: (1) high efficiency in degrading mycotoxins; (2) good probiotic properties, strong growth performance, strong acid tolerance, bile salt tolerance and osmotic pressure tolerance; (3) good hydrophobicity and self-aggregation performance; and (4) high safety.