115 results about "Mycotoxin" patented technology

The invention relates to the technical field of artificial intelligence, and discloses a method and a system for dynamically predicting the risk of harm of feed mycotoxin to livestock and poultry. The method comprises the following steps: constructing a metabolic digital twin model based on a multi-organ coupling dynamics mechanism, connecting a gastrointestinal tract, a liver and a kidney through blood flow parameters to form a closed loop topology network, establishing a toxin migration rate equation, and verifying bioavailability; collecting feed toxin concentration and livestock and poultry physiological data in real time; after distributing the initial toxin load, iteratively generating target organ dynamic concentration distribution by adopting a Runge-Kutta method; an organ exposure index is calculated based on an organ specific hazard threshold, key organs are positioned, and low / high risk levels and toxin accumulation paths are output. The method solves the problems that a traditional static model is low in efficiency and poor in organ difference adaptability when being used for dynamically predicting the risk of harm to livestock and poultry caused by feed mycotoxin.

The invention discloses a pretreatment device for grain mycotoxin detection in the technical field of mycotoxin detection, and the pretreatment device comprises a base, a grinding groove assembly and a first liquid cooling assembly are arranged in the base, a supporting rod is fixedly connected to the base, a grinding head assembly is hinged to the supporting rod, and a second liquid cooling assembly and a liquid supply assembly are arranged in the grinding head assembly. A controller, an extraction liquid storage tank and a cooling liquid storage tank are fixedly connected to the side wall of the base, and the grinding groove assembly, the first liquid cooling assembly, the grinding head assembly, the second liquid cooling assembly and the liquid supply assembly are all in signal connection with the controller. The device is novel and effective, a low-temperature-resistant extracting agent is adaptively supplied while the to-be-detected sample is ground and crushed at a low temperature by the grinding head, the grinding head is rinsed to improve the grinding efficiency, and meanwhile, the extraction effect of the extracting agent on the to-be-detected sample mycotoxin is enhanced by virtue of pressure and shearing force during grinding, so that the accuracy of a subsequent detection result is ensured; the detection work efficiency is improved, and the detection time and difficulty are reduced.

The invention provides a core-shell microsphere for feed detoxification and a preparation method and application thereof.The core-shell microsphere comprises a sclerotium layer, a magnetic shell layer and a catalytic layer from inside to outside, and the sclerotium layer is composed of bacillus licheniformis and lactobacillus rhamnosus composite flora and a chitosan-sodium tripolyphosphate semipermeable membrane wrapping thalli; the magnetic shell layer is a Fe3O4 nano-particle coating, and the catalytic layer is a porous Mn-Ce-O shell. The microspheres have the double functions of biodegradation and chemical catalysis, the internal flora can degrade various toxins, the external catalytic layer efficiently decomposes stubborn toxins, the magnetic layer realizes rapid magnetic separation and recovery, and the microspheres are suitable for removing mycotoxins from feed raw materials such as corn and soybean meal.

The invention provides a montmorillonite-based nano-composite antibacterial mildew-removing agent and a preparation and application method thereof, the preparation method comprises the following steps: crushing bentonite raw ore, grinding and removing impurities to obtain montmorillonite ore powder; the preparation method comprises the following steps: proportioning montmorillonite mineral powder, zinc salt and water in parts by mass, and performing inorganic modification to obtain a montmorillonite-based antibacterial agent filter cake; and proportioning the montmorillonite-based antibacterial agent filter cake, an organic modifier and water in parts by mass, and performing organic modification to obtain the montmorillonite-based composite antibacterial mildew removal agent. The preparation method is simple in process, rich in raw material reserves and easy for large-scale production; the montmorillonite-based nano-composite antibacterial mildew-removing agent prepared by the invention not only can obviously inhibit gram-negative bacteria and gram-positive bacteria, but also can synergistically adsorb different types of mycotoxins, at the same time is beneficial to protection of gastrointestinal mucosa of livestock, and the dependence of animals on hormone drugs such as antibiotics is solved.

The invention relates to the technical field of intelligent detection, in particular to a feed mycotoxin detection method and system based on spectral analysis. The method comprises the following steps: acquiring a near-infrared diffuse reflection spectrum of a feed sample to be detected, constructing an original spectrum vector based on absorbance data of the near-infrared diffuse reflection spectrum, and determining a projection component of the original spectrum vector on a pre-constructed qualified matrix space; subtracting the projection component from the original spectrum vector to obtain a residual vector, and calculating a first-order difference spectrum of the residual vector based on a preset sensitivity weight; and performing local feature weighting on the first-order difference spectrum to determine a weighted feature value, determining a residual entropy value of the residual vector and a range value of the residual vector, and determining a detection result of the to-be-detected feed sample by using the weighted feature value, the residual entropy value and the range value. According to the technical scheme, a more accurate detection result of the mycotoxin of the feed can be obtained.

The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.

Extract of Saccharomyces Cerevisiae Yeast Cell Walls Rich in β-Glucans for the Prevention of the Effects of the Mycotoxin Deoxynivalenol (DON) The present invention relates to extracts of Saccharomyces cerevisiae yeast cell walls rich in β-glucans, and their use in the prevention of mycotoxicoses caused by the mycotoxin deoxynivalenol, in particular in the prevention of liver and intestinal damage, and the immunotoxic effects caused by this mycotoxin. (no figure)

The invention discloses a biological agent for efficiently degrading mycotoxin, a method and application. Belongs to the technical field of enzyme biological preparations. The novel laccase is obtained from a Ganoderma lucidum culture solution and is named as GL-Lac, the amino acid sequence of the novel laccase is shown as SEQ ID NO.1, the novel laccase can effectively degrade AFB1, ZEN, DON, T-2 and other mycotoxins, non-phenolic toxin-AFB1 can be efficiently oxidized without additional mediators, in addition, by means of a composite induction strategy, the degradation rates of AFB1, ZEN, DON and T-2 toxins are remarkably increased and reach 80% or above, 90% or above, 78% or above and 75% or above respectively, and the novel laccase is suitable for industrial production. And a technical support is provided for degradation of mycotoxin.

The application discloses a suspension for improving DON-caused piglet intestinal toxicity and a preparation method and application thereof, and belongs to the technical field of feed additives, and comprises the following raw materials: hesperidin 40-60 mg / mL, sodium butyrate 50-150 mg / mL and PR-39 antibacterial peptide 10-15 mg / mL. The suspension provided by the application can also regulate intestinal flora balance, promote the proliferation of beneficial bacteria such as lactobacillus, and inhibit the growth of harmful bacteria. The effect of the suspension provided by the application is significantly better than that of single component or partial combination, and the suspension provides an efficient and safe nutritional intervention strategy for preventing and treating animal body damage caused by feed mycotoxin pollution.

The invention belongs to the technical field of wine brewing, relates to a natural sweet wine low-sulfur brewing method for delaying white grapes until the white grapes turn red and then harvesting the white grapes, solves the health hidden danger and flavor defects caused by high-sulfur addition in the traditional sweet white wine process, and remarkably improves the flavor quality of the wine at the same time. According to the method, complete and healthy red (total anthocyanin content is greater than or equal to 5mg / kg) fruits are selected through artificial spike selection and grain selection, and the cardinal number of infectious microbes in raw materials is reduced; no commercial yeast is added, natural slow fermentation is achieved by means of microorganisms, and the finished product is good in flavor and quality; gallic acid, condensed tannin and hydrolyzed tannin with a ratio of 1: 1: 1 are compounded to prepare wine-brewing tannin, and the wine-brewing tannin and low-dose sulfur dioxide construct a synergistic system, so that the sulfur consumption of sulfur dioxide is greatly reduced, secondary fermentation of high-sugar wine liquid is prevented, and infectious microbes and wine body oxidation are effectively inhibited. The finished product biogenic amine, pesticide and heavy metal residues all meet the safety standard, mycotoxin is not detected, meanwhile, the original flavor of the wine body is prominent, the storage stability is good, and dual guarantee of health and quality is achieved.

The present invention discloses a feed raw material mycotoxin rapid detection apparatus, and relates to the technical field of mycotoxin detection, the feed raw material mycotoxin rapid detection apparatus comprises a protection assembly, a detection assembly and a stirring assembly, the detection assembly is used for detecting the mycotoxin content in a feed raw material, and the stirring assembly comprises a crushing mechanism, a mixing mechanism, a driving mechanism and a rotating mechanism. According to the feed raw material mycotoxin rapid detection device, a servo motor is started, a bucket-shaped tooth column is driven to rotate, feed and an extracting solution are crushed and mixed at the same time, a hydraulic cylinder is started, and the meshing position of a driving gear and the bucket-shaped tooth column is adjusted, so that the purpose of adjusting the crushing and mixing speed is achieved; the crushing speed is slowed down, and the crushing speed is increased when blocky feed raw materials are crushed, so that the problem that the crushing speed cannot be adjusted according to the texture of the feed raw materials by the existing mycotoxin detection equipment is solved, and the mycotoxin detection efficiency is improved.

The invention belongs to the technical field of fungaltoxin detection, and particularly relates to a Raman beacon COF nanoflower and a preparation method and application thereof. The method comprises the following steps: dissolving trialdehyde phloroglucinol, o-bitoluidine and polyvinylpyrrolidone in methanol, carrying out ultrasonic uniform mixing, adding a catalyst to carry out a reaction, adding benzaldehyde after the reaction is finished, continuously carrying out a standing reaction, centrifuging, washing and redissolving to obtain TpDBD-COF nanoflowers, dissolving the TpDBD-COF nanoflowers in a mixed solution of ethanol and water, adding AgNO3, carrying out ultrasonic adsorption, and carrying out vacuum drying to obtain the TpDBD-COF nanoflowers. After adsorption is finished, trisodium citrate is added for a heating reaction, centrifugation, washing and redissolution are performed, and the Raman beacon COF nanoflower is obtained. The COF nanoflower prepared by the invention has a highly enhanced stable Raman signal, the surface group has the advantage of being directly used for biomolecule coupling, and the COF nanoflower can be specifically used for sensitively detecting T-2 toxin in a sample.

The application discloses a 2-cyanoacrylate compound represented by a general formula (I): each substituent is shown in the specification. The application also discloses a preparation method and application of the 2-cyanoacrylate compound. The 2-cyanoacrylate compound is suitable for inhibiting mycotoxins and preventing diseases caused by pollution fungi.

The invention discloses high-protein micro-capsule coated emulsified fat powder and a preparation method thereof, and belongs to the field of biotechnology processing. The high-protein micro-capsule coated emulsified fat powder comprises the following raw materials in parts by mass: 10-14 parts of fermentation slurry, 8-12 parts of vegetable oil, 40-60 parts of composite wall material raw materials, 0.5-4 parts of an emulsifier A, 1.5-3 parts of an emulsifier B, 4-10 parts of a polysaccharide compound and 2-6 parts of other auxiliaries, the composite wall material comprises the following raw materials: glycosylated protein and hydrophobic modified maltodextrin in a mass ratio of 1: (2-3). The micromolecular nutrient substances in the fermented slurry can improve the nutrient digestion and absorption rate of livestock and poultry, reduce the pollution of mycotoxin, and can effectively protect the active components as the microcapsule core material. The raw materials of the composite wall material well adapt to a composite core material system, the embedding rate of fermentation slurry is improved, a continuous and compact wall shell is obtained, internal active ingredients are effectively protected, and slow release of the core material in intestinal tracts is controlled.

The application relates to the technical field of plant protection, in particular to application of ferroptosis inhibitor-1 in preparation of a toxitant inhibitor of mycotoxin and a pesticide composition. The application finds that the ferroptosis inhibitor-1 can significantly improve the tolerance of plants to mycotoxin poisoning, has the effect of relieving the plants from mycotoxin poisoning, and helps the plants and seeds to normally germinate and grow under the condition of high-concentration mycotoxin.

The invention provides a harmless treatment method and application of chicken manure, and relates to the technical field of waste recycling. Specifically, the method comprises an ozone oxidation process, a drying process, an enzymolysis process, an aerobic fermentation process and an anaerobic fermentation process which are carried out in sequence, wherein in the aerobic fermentation process, a pre-product is mixed with charcoal, a cellulose source, plant ash, wine grape skin and rice bran meal for fermentation; in the anaerobic fermentation process, compound bacteria including clostridium butyricum, pediococcus acidilactici, bacillus coagulans and enterococcus faecium are added into a pre-product, and sealed fermentation is performed. The chicken manure is subjected to targeted detoxification treatment according to the characteristics of the chicken manure, pathogenic microorganisms and complex heavy metals in the chicken manure can be effectively removed, the content of mycotoxin in the chicken manure is reduced, nutrient substances in the chicken manure are decomposed, resource application in the field of fertilizer or feed is achieved, and the method has good application prospects.

The invention belongs to the technical field of aquatic feed protein sources, and particularly relates to a method for increasing the protein content of DDGS (Distillers Dried Grains with Soluble). The method comprises the following steps: carrying out enzymolysis on corn DDGS by adopting hydrolase, and then carrying out enzyme inactivation, centrifugal separation and drying to obtain corn DDGS concentrated protein; the hydrolase is prepared from one or more of cellulase, xylanase, beta-glucolase, beta-glucosidase and cellobiase. According to the method, the protein content is increased through enzymolysis, and possible adverse effects of microorganisms used in a traditional method for increasing the DDGS protein content through microbial fermentation and metabolites of the microorganisms on the health of aquatic animals can be avoided. Meanwhile, the mycotoxin content of the DDGS can be reduced, and the vomitoxin content of the DDGS is further reduced from 2mg / kg to a degree which cannot be detected.

This application belongs to the technical field of mycotoxin detection, and specifically relates to the application of a magnetic solid-phase dispersion extractant in detecting the mycotoxin content in wheat. The magnetic solid-phase dispersion extractant is NH2-Fe3O4@TpBD-COF. As a magnetic solid-phase microextraction agent, NH2-Fe3O4@TpBD-COF adopted in the present invention only needs 10 minutes to complete the treatment process in the technical solution provided by the present invention, featuring a short required time; compared with the commonly used solid-phase microextraction column, the technical solution provided by the present invention only requires a usage amount of 10 mg, featuring low cost; compared with the disposable extraction column, NH2-Fe3O4@TpBD-COF adopted in the present invention can be reused 8 times after simple cleaning, featuring environmental friendliness.

The utility model relates to the technical field of equipment special for preparing and processing livestock feed, in particular to a feed mycotoxin removing device which comprises a multi-stage mixing assembly. The multi-stage mixing assembly comprises a mixing protection shell as well as a first-stage mixer, a first-stage transition layer, a second-stage mixer, a second-stage transition layer, a third-stage mixer, a third-stage transition layer and a fourth-stage mixer which are sequentially connected in the mixing protection shell from top to bottom, and the mixing protection shell is communicated with a liquid conveying pipe; the first-stage transition layer, the second-stage transition layer and the third-stage transition layer are all connected with the liquid conveying pipe, and a feeding opening is formed in the top of the mixing protection shell. Through the mixing protection shell, the first-stage mixer, the second-stage mixer, the third-stage mixer, the fourth-stage mixer, the liquid conveying pipe and the feeding opening, multi-stage and multi-batch operation is achieved, and the problem that in the prior art, due to the fact that the activity amount of part of feed is small, the mycotoxin removal effect is poor is solved.

This invention relates to the field of microfluidic sensing and detection technology, and more particularly to a photoelectric / fluorescence dual-mode self-calibrating microfluidic sensor for the detection of various mycotoxins, its fabrication method, and its application. The microfluidic sensor comprises a three-channel microfluidic chip and a substrate. In its fabrication, the three independent working electrodes and an integrated reference-counter electrode system are integrated into the microfluidic chip, utilizing oxygen-rich vacancy Io. 0.2~0.4 S 0.3~0.5 Using BiOBr (ISBO-OVs)@Au composite material as the sensing substrate, an internal secondary light source is formed by the chemiluminescence reaction of luminol-superoxide radicals, which can cascade and amplify the photocurrent signal. At the same time, combined with the specific recognition function of 7DCCA labeled aptamers, the sensitivity and specificity of microfluidic sensors for detecting trace fungal toxins are greatly improved.

A variety of potentially harmful substances can be removed from alcoholic and non-alcoholic beverages as well as condiments using a pack that includes a variety of ingredients within a liquid-permeable housing. The pack is immersed in a liquid that needs to be detoxified, with the ingredients binding to the potentially harmful compounds within the liquid, and after a desired period of time, the pack is separated from the liquid, thus removing from the potentially harmful compounds that are bound to the ingredients from the liquid. When combined within a single compartment of the housing, the ingredients display synergy in removing of many of the potentially harmful compounds. Additionally, the packs can be enhanced for removal of particular sets of compounds, such as environmental pollutants, illicit substances, mold and mycotoxins, food additives, and additional alcohol toxins.

A Ti—Al—Zr—O metal oxide having the formula TixAlyZrzOn, wherein x is independently 2-10, y is independently 0.5-6, z is independently 2-10, and n is independently 2x+3y / 2+2z. A method of separating a mycotoxin from a matrix using a Ti—Al—Zr—O metal oxide.

The present invention relates to a composition for controlling plant pathogenic fungi, which contains the Bacillus velezensis JCK-7158 strain, a culture solution thereof, or an extract thereof, a method for producing the composition, and a method for controlling plant pathogenic fungi. The culture solution or extract thereof of the strain has remarkably excellent antifungal activity and induced resistance, and is excellent in the ability to reduce toxins produced by fungi, and therefore can be used to control plant pathogenic fungi.

This application belongs to the technical field of mycotoxin detection technology, specifically relating to a Raman beacon COF nanoflower, its preparation method, and its application. The method involves dissolving trialdehyde phloroglucinol, o-toluidine, and polyvinylpyrrolidone in methanol, ultrasonically mixing, adding a catalyst for reaction, adding benzaldehyde after the reaction, allowing the mixture to stand, centrifuging, washing, and redissolving to obtain TpDBD-COF nanoflowers. These nanoflowers are then dissolved in a mixed solution of ethanol and water, followed by the addition of AgNO3 and ultrasonic adsorption. After adsorption, trisodium citrate is added for heating reaction, centrifuging, washing, and redissolving to obtain the Raman beacon COF nanoflowers. The COF nanoflowers prepared by this invention exhibit a highly enhanced and stable Raman signal, and their surface groups have the advantage of being directly used for biomolecule coupling, specifically for the sensitive detection of T-2 toxin in samples.

The invention discloses a composite mildew preventive for feed and a preparation method of the composite mildew preventive, and belongs to the technical field of preparation of composite mildew preventive. Comprising the following steps: carrying out centrifugal separation on beer yeast paste to remove impurities so as to obtain solid yeast, carrying out ultrasonic oscillation and two-stage enzymolysis on the solid yeast, carrying out vacuum drying so as to obtain a yeast extract, and pretreating the yeast extract so as to obtain an alkaline yeast extract solution; the preparation method comprises the following steps: mixing an alkaline yeast extract solution and sodium bromoacetate, stirring and reacting to obtain a modified yeast extract solution, adjusting the pH value of the modified yeast extract solution, filtering to obtain a modified yeast extract, mixing the modified yeast extract, cinnamyl aldehyde, ammonium propionate and montmorillonite K10, and crushing to obtain the composite mildew preventive for feed. By synergistically reducing the number of mould and the content of mycotoxin in the feed, the effect of the composite mildew preventive for the feed is improved.

This invention belongs to the field of native shrub seedling propagation technology, and discloses a system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions. The system includes a data processing platform, acquisition equipment, and control equipment. The data processing platform establishes habitat and germplasm archives corresponding to the same germplasm batch and sequentially completes germplasm dormancy treatment, staged seedling management, root culture, mycotoxin-seedling symbiosis, stress substrate integration, and planting feedback. The method generates qualified germplasm records and germination initiation records based on germplasm status and dormancy type. Seedling stage status records serve as the basis for initiating the next stage. Root culture, mycotoxin inoculation, stress pre-adaptation, and substrate transition are performed based on the habitat information of the target restoration plot. After planting, survival rate, greening time, new shoot growth, and overwintering survival rate are recorded in the habitat and germplasm archive to correct parameters for subsequent batches.

The factory-like multi-path feeding equipment comprises a collecting box, the two sides of the collecting box are rotationally connected with first rotating plates respectively, a discharging assembly is fixedly installed on the inner bottom wall of the collecting box, and discharging assemblies are arranged on the two sides of the collecting box respectively; the discharging assembly comprises a supporting frame and an electric push rod. According to the factory-like multi-path feeding equipment, through the arrangement of the discharging assembly, a good discharging effect can be achieved, discharging is easy and convenient, residues do not exist after discharging, it is guaranteed that fed feed is fresh and free of pollution through accurate and residue-free discharging, and the feeding efficiency is improved. The risk that harmful substances such as mycotoxin generated by deterioration of residual feed are eaten by cultured organisms by mistake is avoided, so that the normal functions of digestive systems of the cultured organisms can be maintained, the probability that the cultured organisms suffer from health problems such as intestinal diseases is reduced, the cultured organisms can thrive in a healthy state, and the overall survival rate and the culture quality are improved.

This invention discloses a test strip, reagent kit, and method for simultaneous detection of multiple mycotoxins in feed based on self-calibrated SERS lateral flow chromatography, belonging to the field of biodetection technology. The test strip includes a backing plate, a sample pad, a conjugate pad, an NC membrane, and an absorbent pad. The NC membrane is adhered to the backing plate, and the absorbent pad and the conjugate pad are respectively tightly pressed against both ends of the NC membrane. The sample pad is tightly pressed against the end of the conjugate pad furthest from the NC membrane. From the end closest to the conjugate pad to the end furthest from the conjugate pad, the NC membrane has a control line and at least one detection line sequentially arranged. This test strip based on self-calibrated SERS lateral flow chromatography has high detection efficiency, with advantages such as fast detection speed, high sensitivity, strong specificity, and simple operation. It can achieve simultaneous detection of multiple mycotoxins and is suitable for rapid detection in feed production sites and grassroots supervision.

The application discloses a ricin toxin detection sensor based on competitive binding of ricin to mitochondrial adenine nucleotide translocator (ANT) and a detection method, which comprises ANT protein, hollow dialysis fiber, chemical cross-linking agent and fluorescently labeled ATP. When in use, the ANT is first fixed on the inner wall of the hollow fiber dialysis membrane and the fluorescently labeled ATP (cy5-12-ATP or cy3-12-ATP or DEPC-12-ATP) is added; then, it is placed in the to-be-detected solution for 30-60 seconds, and then is placed under a fluorescence detection instrument (such as a fluorescence microscope) for observation, so that the presence or absence of the ricin toxin and the approximate concentration of the toxin are determined; then, the relative intensity of the fluorescence of the to-be-detected solution is determined; a standard curve of the corresponding relationship between the concentration of the fluorescent group and the concentration of the toxin is established; and the concentration of the ricin toxin in the to-be-detected solution is obtained by substituting the concentration of the fluorescent group of the to-be-detected solution into the above standard curve and performing calculation. The application can effectively improve the detection efficiency, simplify the detection method and be widely applied in the society.

The application discloses a kind of full-automatic crushing detection integrated machine of grain granular material, it is related to detection technical field, including constant volume module, huller module, microwave heating and moisture measurement module etc.;Constant volume module is transferred to the original grain of upper sampling, is handled by fixed volume container constant volume, and the surplus original grain is thrown away, huller module is used to shell processing to the original grain, and then it is transferred to microwave heating and moisture measurement module by mechanical hand module after original grain is shelled;The application integrates all modules from the original grain feeding to the result detection;Using mechanical arm transition, successfully realizes the full-automatic operation from original grain receiving to result output. Through crushing module, the materials in different positions in the crushing box can be crushed, so that the contact area of the mycotoxins and heavy metals inside the grain is larger during extraction, and the corresponding food safety index detection is more accurate during detection.