80 results about "Mycotoxin" patented technology

The invention provides a core-shell microsphere for feed detoxification and a preparation method and application thereof.The core-shell microsphere comprises a sclerotium layer, a magnetic shell layer and a catalytic layer from inside to outside, and the sclerotium layer is composed of bacillus licheniformis and lactobacillus rhamnosus composite flora and a chitosan-sodium tripolyphosphate semipermeable membrane wrapping thalli; the magnetic shell layer is a Fe3O4 nano-particle coating, and the catalytic layer is a porous Mn-Ce-O shell. The microspheres have the double functions of biodegradation and chemical catalysis, the internal flora can degrade various toxins, the external catalytic layer efficiently decomposes stubborn toxins, the magnetic layer realizes rapid magnetic separation and recovery, and the microspheres are suitable for removing mycotoxins from feed raw materials such as corn and soybean meal.

The invention relates to the technical field of intelligent detection, in particular to a feed mycotoxin detection method and system based on spectral analysis. The method comprises the following steps: acquiring a near-infrared diffuse reflection spectrum of a feed sample to be detected, constructing an original spectrum vector based on absorbance data of the near-infrared diffuse reflection spectrum, and determining a projection component of the original spectrum vector on a pre-constructed qualified matrix space; subtracting the projection component from the original spectrum vector to obtain a residual vector, and calculating a first-order difference spectrum of the residual vector based on a preset sensitivity weight; and performing local feature weighting on the first-order difference spectrum to determine a weighted feature value, determining a residual entropy value of the residual vector and a range value of the residual vector, and determining a detection result of the to-be-detected feed sample by using the weighted feature value, the residual entropy value and the range value. According to the technical scheme, a more accurate detection result of the mycotoxin of the feed can be obtained.

The invention discloses a strain of Bacteroides ovatus L2 and application of the strain of Bacteroides ovatus L2 in a product for preventing and treating vomitoxin toxicity. The strain is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC.No: 67205, and the preservation date is November 4, 2025. The strain is separated and purified from a healthy adult excrement sample, has the characteristics of good acid resistance, cholate resistance, gastrointestinal fluid resistance and the like, and is free of hemolysis and good in safety. The strain can effectively relieve the toxic effect induced by vomitoxin, mainly inhibits inflammatory factors and promotes expression of intestinal barrier functional proteins, can be applied to products of microecologics for preventing and treating DON toxicity, and has a good application prospect.

Extract of Saccharomyces Cerevisiae Yeast Cell Walls Rich in β-Glucans for the Prevention of the Effects of the Mycotoxin Deoxynivalenol (DON) The present invention relates to extracts of Saccharomyces cerevisiae yeast cell walls rich in β-glucans, and their use in the prevention of mycotoxicoses caused by the mycotoxin deoxynivalenol, in particular in the prevention of liver and intestinal damage, and the immunotoxic effects caused by this mycotoxin. (no figure)

The application discloses a suspension for improving DON-caused piglet intestinal toxicity and a preparation method and application thereof, and belongs to the technical field of feed additives, and comprises the following raw materials: hesperidin 40-60 mg / mL, sodium butyrate 50-150 mg / mL and PR-39 antibacterial peptide 10-15 mg / mL. The suspension provided by the application can also regulate intestinal flora balance, promote the proliferation of beneficial bacteria such as lactobacillus, and inhibit the growth of harmful bacteria. The effect of the suspension provided by the application is significantly better than that of single component or partial combination, and the suspension provides an efficient and safe nutritional intervention strategy for preventing and treating animal body damage caused by feed mycotoxin pollution.

The invention belongs to the technical field of wine brewing, relates to a natural sweet wine low-sulfur brewing method for delaying white grapes until the white grapes turn red and then harvesting the white grapes, solves the health hidden danger and flavor defects caused by high-sulfur addition in the traditional sweet white wine process, and remarkably improves the flavor quality of the wine at the same time. According to the method, complete and healthy red (total anthocyanin content is greater than or equal to 5mg / kg) fruits are selected through artificial spike selection and grain selection, and the cardinal number of infectious microbes in raw materials is reduced; no commercial yeast is added, natural slow fermentation is achieved by means of microorganisms, and the finished product is good in flavor and quality; gallic acid, condensed tannin and hydrolyzed tannin with a ratio of 1: 1: 1 are compounded to prepare wine-brewing tannin, and the wine-brewing tannin and low-dose sulfur dioxide construct a synergistic system, so that the sulfur consumption of sulfur dioxide is greatly reduced, secondary fermentation of high-sugar wine liquid is prevented, and infectious microbes and wine body oxidation are effectively inhibited. The finished product biogenic amine, pesticide and heavy metal residues all meet the safety standard, mycotoxin is not detected, meanwhile, the original flavor of the wine body is prominent, the storage stability is good, and dual guarantee of health and quality is achieved.

The present invention discloses a feed raw material mycotoxin rapid detection apparatus, and relates to the technical field of mycotoxin detection, the feed raw material mycotoxin rapid detection apparatus comprises a protection assembly, a detection assembly and a stirring assembly, the detection assembly is used for detecting the mycotoxin content in a feed raw material, and the stirring assembly comprises a crushing mechanism, a mixing mechanism, a driving mechanism and a rotating mechanism. According to the feed raw material mycotoxin rapid detection device, a servo motor is started, a bucket-shaped tooth column is driven to rotate, feed and an extracting solution are crushed and mixed at the same time, a hydraulic cylinder is started, and the meshing position of a driving gear and the bucket-shaped tooth column is adjusted, so that the purpose of adjusting the crushing and mixing speed is achieved; the crushing speed is slowed down, and the crushing speed is increased when blocky feed raw materials are crushed, so that the problem that the crushing speed cannot be adjusted according to the texture of the feed raw materials by the existing mycotoxin detection equipment is solved, and the mycotoxin detection efficiency is improved.

The invention belongs to the technical field of fungaltoxin detection, and particularly relates to a Raman beacon COF nanoflower and a preparation method and application thereof. The method comprises the following steps: dissolving trialdehyde phloroglucinol, o-bitoluidine and polyvinylpyrrolidone in methanol, carrying out ultrasonic uniform mixing, adding a catalyst to carry out a reaction, adding benzaldehyde after the reaction is finished, continuously carrying out a standing reaction, centrifuging, washing and redissolving to obtain TpDBD-COF nanoflowers, dissolving the TpDBD-COF nanoflowers in a mixed solution of ethanol and water, adding AgNO3, carrying out ultrasonic adsorption, and carrying out vacuum drying to obtain the TpDBD-COF nanoflowers. After adsorption is finished, trisodium citrate is added for a heating reaction, centrifugation, washing and redissolution are performed, and the Raman beacon COF nanoflower is obtained. The COF nanoflower prepared by the invention has a highly enhanced stable Raman signal, the surface group has the advantage of being directly used for biomolecule coupling, and the COF nanoflower can be specifically used for sensitively detecting T-2 toxin in a sample.

The application discloses a 2-cyanoacrylate compound represented by a general formula (I): each substituent is shown in the specification. The application also discloses a preparation method and application of the 2-cyanoacrylate compound. The 2-cyanoacrylate compound is suitable for inhibiting mycotoxins and preventing diseases caused by pollution fungi.

The invention discloses high-protein micro-capsule coated emulsified fat powder and a preparation method thereof, and belongs to the field of biotechnology processing. The high-protein micro-capsule coated emulsified fat powder comprises the following raw materials in parts by mass: 10-14 parts of fermentation slurry, 8-12 parts of vegetable oil, 40-60 parts of composite wall material raw materials, 0.5-4 parts of an emulsifier A, 1.5-3 parts of an emulsifier B, 4-10 parts of a polysaccharide compound and 2-6 parts of other auxiliaries, the composite wall material comprises the following raw materials: glycosylated protein and hydrophobic modified maltodextrin in a mass ratio of 1: (2-3). The micromolecular nutrient substances in the fermented slurry can improve the nutrient digestion and absorption rate of livestock and poultry, reduce the pollution of mycotoxin, and can effectively protect the active components as the microcapsule core material. The raw materials of the composite wall material well adapt to a composite core material system, the embedding rate of fermentation slurry is improved, a continuous and compact wall shell is obtained, internal active ingredients are effectively protected, and slow release of the core material in intestinal tracts is controlled.

The application relates to the technical field of plant protection, in particular to application of ferroptosis inhibitor-1 in preparation of a toxitant inhibitor of mycotoxin and a pesticide composition. The application finds that the ferroptosis inhibitor-1 can significantly improve the tolerance of plants to mycotoxin poisoning, has the effect of relieving the plants from mycotoxin poisoning, and helps the plants and seeds to normally germinate and grow under the condition of high-concentration mycotoxin.

The invention belongs to the technical field of aquatic feed protein sources, and particularly relates to a method for increasing the protein content of DDGS (Distillers Dried Grains with Soluble). The method comprises the following steps: carrying out enzymolysis on corn DDGS by adopting hydrolase, and then carrying out enzyme inactivation, centrifugal separation and drying to obtain corn DDGS concentrated protein; the hydrolase is prepared from one or more of cellulase, xylanase, beta-glucolase, beta-glucosidase and cellobiase. According to the method, the protein content is increased through enzymolysis, and possible adverse effects of microorganisms used in a traditional method for increasing the DDGS protein content through microbial fermentation and metabolites of the microorganisms on the health of aquatic animals can be avoided. Meanwhile, the mycotoxin content of the DDGS can be reduced, and the vomitoxin content of the DDGS is further reduced from 2mg / kg to a degree which cannot be detected.

The utility model relates to the technical field of equipment special for preparing and processing livestock feed, in particular to a feed mycotoxin removing device which comprises a multi-stage mixing assembly. The multi-stage mixing assembly comprises a mixing protection shell as well as a first-stage mixer, a first-stage transition layer, a second-stage mixer, a second-stage transition layer, a third-stage mixer, a third-stage transition layer and a fourth-stage mixer which are sequentially connected in the mixing protection shell from top to bottom, and the mixing protection shell is communicated with a liquid conveying pipe; the first-stage transition layer, the second-stage transition layer and the third-stage transition layer are all connected with the liquid conveying pipe, and a feeding opening is formed in the top of the mixing protection shell. Through the mixing protection shell, the first-stage mixer, the second-stage mixer, the third-stage mixer, the fourth-stage mixer, the liquid conveying pipe and the feeding opening, multi-stage and multi-batch operation is achieved, and the problem that in the prior art, due to the fact that the activity amount of part of feed is small, the mycotoxin removal effect is poor is solved.

This invention relates to the field of microfluidic sensing and detection technology, and more particularly to a photoelectric / fluorescence dual-mode self-calibrating microfluidic sensor for the detection of various mycotoxins, its fabrication method, and its application. The microfluidic sensor comprises a three-channel microfluidic chip and a substrate. In its fabrication, the three independent working electrodes and an integrated reference-counter electrode system are integrated into the microfluidic chip, utilizing oxygen-rich vacancy Io. 0.2~0.4 S 0.3~0.5 Using BiOBr (ISBO-OVs)@Au composite material as the sensing substrate, an internal secondary light source is formed by the chemiluminescence reaction of luminol-superoxide radicals, which can cascade and amplify the photocurrent signal. At the same time, combined with the specific recognition function of 7DCCA labeled aptamers, the sensitivity and specificity of microfluidic sensors for detecting trace fungal toxins are greatly improved.

A variety of potentially harmful substances can be removed from alcoholic and non-alcoholic beverages as well as condiments using a pack that includes a variety of ingredients within a liquid-permeable housing. The pack is immersed in a liquid that needs to be detoxified, with the ingredients binding to the potentially harmful compounds within the liquid, and after a desired period of time, the pack is separated from the liquid, thus removing from the potentially harmful compounds that are bound to the ingredients from the liquid. When combined within a single compartment of the housing, the ingredients display synergy in removing of many of the potentially harmful compounds. Additionally, the packs can be enhanced for removal of particular sets of compounds, such as environmental pollutants, illicit substances, mold and mycotoxins, food additives, and additional alcohol toxins.

A Ti—Al—Zr—O metal oxide having the formula TixAlyZrzOn, wherein x is independently 2-10, y is independently 0.5-6, z is independently 2-10, and n is independently 2x+3y / 2+2z. A method of separating a mycotoxin from a matrix using a Ti—Al—Zr—O metal oxide.

The present invention relates to a composition for controlling plant pathogenic fungi, which contains the Bacillus velezensis JCK-7158 strain, a culture solution thereof, or an extract thereof, a method for producing the composition, and a method for controlling plant pathogenic fungi. The culture solution or extract thereof of the strain has remarkably excellent antifungal activity and induced resistance, and is excellent in the ability to reduce toxins produced by fungi, and therefore can be used to control plant pathogenic fungi.

This application belongs to the technical field of mycotoxin detection technology, specifically relating to a Raman beacon COF nanoflower, its preparation method, and its application. The method involves dissolving trialdehyde phloroglucinol, o-toluidine, and polyvinylpyrrolidone in methanol, ultrasonically mixing, adding a catalyst for reaction, adding benzaldehyde after the reaction, allowing the mixture to stand, centrifuging, washing, and redissolving to obtain TpDBD-COF nanoflowers. These nanoflowers are then dissolved in a mixed solution of ethanol and water, followed by the addition of AgNO3 and ultrasonic adsorption. After adsorption, trisodium citrate is added for heating reaction, centrifuging, washing, and redissolving to obtain the Raman beacon COF nanoflowers. The COF nanoflowers prepared by this invention exhibit a highly enhanced and stable Raman signal, and their surface groups have the advantage of being directly used for biomolecule coupling, specifically for the sensitive detection of T-2 toxin in samples.

This invention belongs to the field of native shrub seedling propagation technology, and discloses a system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions. The system includes a data processing platform, acquisition equipment, and control equipment. The data processing platform establishes habitat and germplasm archives corresponding to the same germplasm batch and sequentially completes germplasm dormancy treatment, staged seedling management, root culture, mycotoxin-seedling symbiosis, stress substrate integration, and planting feedback. The method generates qualified germplasm records and germination initiation records based on germplasm status and dormancy type. Seedling stage status records serve as the basis for initiating the next stage. Root culture, mycotoxin inoculation, stress pre-adaptation, and substrate transition are performed based on the habitat information of the target restoration plot. After planting, survival rate, greening time, new shoot growth, and overwintering survival rate are recorded in the habitat and germplasm archive to correct parameters for subsequent batches.

The factory-like multi-path feeding equipment comprises a collecting box, the two sides of the collecting box are rotationally connected with first rotating plates respectively, a discharging assembly is fixedly installed on the inner bottom wall of the collecting box, and discharging assemblies are arranged on the two sides of the collecting box respectively; the discharging assembly comprises a supporting frame and an electric push rod. According to the factory-like multi-path feeding equipment, through the arrangement of the discharging assembly, a good discharging effect can be achieved, discharging is easy and convenient, residues do not exist after discharging, it is guaranteed that fed feed is fresh and free of pollution through accurate and residue-free discharging, and the feeding efficiency is improved. The risk that harmful substances such as mycotoxin generated by deterioration of residual feed are eaten by cultured organisms by mistake is avoided, so that the normal functions of digestive systems of the cultured organisms can be maintained, the probability that the cultured organisms suffer from health problems such as intestinal diseases is reduced, the cultured organisms can thrive in a healthy state, and the overall survival rate and the culture quality are improved.

This invention discloses a test strip, reagent kit, and method for simultaneous detection of multiple mycotoxins in feed based on self-calibrated SERS lateral flow chromatography, belonging to the field of biodetection technology. The test strip includes a backing plate, a sample pad, a conjugate pad, an NC membrane, and an absorbent pad. The NC membrane is adhered to the backing plate, and the absorbent pad and the conjugate pad are respectively tightly pressed against both ends of the NC membrane. The sample pad is tightly pressed against the end of the conjugate pad furthest from the NC membrane. From the end closest to the conjugate pad to the end furthest from the conjugate pad, the NC membrane has a control line and at least one detection line sequentially arranged. This test strip based on self-calibrated SERS lateral flow chromatography has high detection efficiency, with advantages such as fast detection speed, high sensitivity, strong specificity, and simple operation. It can achieve simultaneous detection of multiple mycotoxins and is suitable for rapid detection in feed production sites and grassroots supervision.

The application discloses a ricin toxin detection sensor based on competitive binding of ricin to mitochondrial adenine nucleotide translocator (ANT) and a detection method, which comprises ANT protein, hollow dialysis fiber, chemical cross-linking agent and fluorescently labeled ATP. When in use, the ANT is first fixed on the inner wall of the hollow fiber dialysis membrane and the fluorescently labeled ATP (cy5-12-ATP or cy3-12-ATP or DEPC-12-ATP) is added; then, it is placed in the to-be-detected solution for 30-60 seconds, and then is placed under a fluorescence detection instrument (such as a fluorescence microscope) for observation, so that the presence or absence of the ricin toxin and the approximate concentration of the toxin are determined; then, the relative intensity of the fluorescence of the to-be-detected solution is determined; a standard curve of the corresponding relationship between the concentration of the fluorescent group and the concentration of the toxin is established; and the concentration of the ricin toxin in the to-be-detected solution is obtained by substituting the concentration of the fluorescent group of the to-be-detected solution into the above standard curve and performing calculation. The application can effectively improve the detection efficiency, simplify the detection method and be widely applied in the society.

The present invention discloses a feed containing a cereal and fruit fermentation composition and a preparation method thereof, and belongs to the field of livestock and poultry feeds, the feed comprises the following components in parts by weight: 50-100 parts of a cereal and fruit fermentation composition, 10-20 parts of synbiotics, 3-7 parts of organic trace elements, 1-5 parts of a mineral premix, and 0.5-3 parts of a vitamin premix, wherein the cereal and fruit fermented composition comprises feed syrup and cereal and fruit wet residues. By using the feed prepared by the invention, the problems that the feed in the prior art cannot provide efficient nutrition supply, protect intestinal health, degrade mycotoxin and efficiently utilize resources can be solved.

The invention relates to the technical field of forage grass ensiling fresh-keeping, in particular to a method for inhibiting growth of mould hyphae in ensiling forage grass, which comprises the following steps: 1) preparing targeted straw vinegar: adopting a dry distillation method, cooling and condensing, standing and layering, filtering and adsorbing and purifying by activated carbon, and accurately controlling the content of acetic acid to be 30-40% and the content of phenolic compounds to be 15-20%; 2) adding a composite probiotic protection system: spraying targeting straw vinegar liquid accounting for 0.5-5% of the weight of the raw materials into the silage raw materials, and synchronously adding 0.1-0.3% of a composite protective agent; 3) regulating and controlling the ensiling environment in stages: the temperature is 25-30 DEG C and the humidity is 60-70% in the first 10 days, the temperature is 20-25 DEG C and the humidity is 60-70% in the later 20-35 days, and the total ensiling time is 30-45 days; and 4) dual detection verification: detecting mycotoxin by HPLC-MS / MS, and detecting the number of beneficial bacteria by a plate counting method.Experiments show that the method has an aspergillus flavus and aspergillus fumigatus inhibition rate of more than 65%, a lactobacillus survival rate of more than 90%, no residue of straw vinegar, and low cost, and is suitable for silage of various forage grass such as corn straw, alfalfa and the like.

The invention discloses a filter membrane with an MOF derived porous structure, a preparation method, a mycotoxin separation and extraction device and a separation and extraction method, and relates to the technical field of mycotoxin pretreatment. A porous functional layer is constructed on the surface of a filter membrane substrate and in pore channels through a controlled conversion process, the porous functional layer has a porous structure containing a Zr-O-C network, microporous channels are reserved, polar functional groups are remained on the surface, and the MOF-derived porous structure filter membrane which does not contain a complete MOF crystal structure is formed. The membrane is used in a circulation type working mode, a target analyte can quickly pass through the membrane layer along with fluid, and the matrix effect can be effectively reduced. The membrane material can realize rapid and simplified sample purification on the premise of ensuring high recovery rate of a target object. The method has the advantages of simple preparation process, stable structure, low flow resistance, wide applicable solvent range and the like, and is particularly suitable for rapid pretreatment analysis of trace micromolecule pollutants in complex food and agricultural product matrixes.

The application discloses a kind of metal organic framework films, preparation method and application thereof, it is related to antibiotic and mycotoxin removal technical field.The preparation method includes the following steps: metal source and organic ligand are added into solvent and mixed uniformly, obtain mixed solution;After using base material adsorbs the mixed solution, by in-situ growth method, in-situ growth polymerization metal organic framework crystal is carried out on the internal fiber surface of the base material, and the metal organic framework film is obtained;The metal source is zirconium salt;The organic ligand is tetra [4- (3,5-dicarboxyphenyl) ] tetraphenyl ethylene.This metal organic framework film can realize the high selectivity adsorption of antibiotic and mycotoxin by specific chemical structure and chemical composition, with the technical advantages of short antibiotic and mycotoxin adsorption time, high effect, low economic cost.

This invention relates to the field of chromatographic detection and agricultural product quality and safety analysis technology, specifically to a system for detecting and analyzing the content of agricultural toxins based on chromatographic separation. It includes: a communication module, a signal acquisition module, a benchmark modeling module, a phase shift calculation module, a kinetic decoupling module, a quantitative analysis module, and a hardware feedback module. By synchronously acquiring spectral intensity, retention time, pump pressure transient pressure, and column oven heat flux gradient signals, a multidimensional physical space tensor is generated to establish a benchmark kinetic manifold for the pure analyte. The phase shift vector of the actual observed trajectory relative to the benchmark kinetic manifold is calculated, separating the global phase shift component from the local high-frequency response component. This invention achieves absolute quantification of the target analyte and matrix inhibition assessment, and inversely drives injection volume adjustment or knowledge base storage.

The application discloses a detection device of a mycotoxin biosensor, which comprises a button, a machine bin, a limiting plate, an elastic piece and a scanning machine. The button is electrically connected with the limiting plate through the machine bin. The limiting plate is transitionally fitted on the elastic piece. The elastic piece is gap-fitted with the scanning machine. A correction device and a pushing structure are arranged on the assisting body. The correction device and the pushing structure are mutually matched on the folding frame. When the spring bending rotary part is buckled on the 90-degree vertical angle of the limiting plate, the penetrating block is pressed on the pushing structure, the pushing structure is opened to the folding frame, the traction belt on the stacking adjusting column is pulled, the correction device is rotated to be attached to each bending rotary part of the spring, is centrally positioned between each bending rotary part, and the stereoscopic sense of the extended spring is effectively controlled.

The invention discloses a sunlight-responsive stirring-free carbon nitride nanosheet photocatalyst capable of efficiently degrading water and surface pollutants. According to the method, melamine is taken as a precursor, KCl is taken as a dispersion medium, defect-state carbon nitride is obtained through high-temperature calcination, chemical shearing and surface carboxylation are carried out through concentrated nitric acid hydro-thermal treatment, and the ultra-dispersed carbon nitride nanosheet is obtained through centrifugal cleaning. The process is simple and low in cost. The size of the obtained nanosheet is dozens of nanometers, the surface of the nanosheet is rich in carboxyl, the dispersity in water is good, and the nanosheet has strong photochemical activity of sunlight response. Under real sunlight, only 50 mu g / mL of a catalyst is needed, stirring and aeration are not needed, and the degradation rate of rhodamine B (the concentration is 75 mu M) and typical mycotoxin aflatoxin B1 (the concentration is 1 ppm) in an aqueous solution exceeds 99% within one hour. And pollutants (including fungaltoxin and dye residues) on the surfaces of traditional Chinese medicinal materials and bulk foods (including peanuts and corns) can be effectively degraded in a spraying or immersing manner.