Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacilli, which is applied in the fields of application, bacteria, and food preservation, and can solve problems such as polluting the environment, affecting food safety, and destroying nutrition

Inactive Publication Date: 2011-09-21
ACAD OF STATE GRAIN ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods have defects such as destruction of nutrition, short duration of effect, incomplete anti-mold and high cost, resulting in unsatisfactory act

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Acquisition and identification of Bacillus amyloliquefaciens ASAG1

[0019] 1. Acquisition and identification of Bacillus amyloliquefaciens ASAG1

[0020] The bacillus amyloliquefaciens described in the present invention is isolated from grain stored corn. The morphology of the bacterial strain is as follows: Gram-positive bacteria, rod-shaped cell, spore-forming, catalase and oxidase reactions are positive. Acid production test using carbohydrates: starch hydrolysis, hydrolyzed casein and hydrolyzed gelatin were all positive. Optimal medium formula: potato 200 g / L, glucose 20 g / L, agar 20 g / L, agar 15 g, natural pH. The culture temperature is 28~32℃.

[0021] The 16S rDNA sequence of the ASAG1 strain of the present invention is shown in SEQ ID No: 1 in the sequence table.

[0022] 2. Preservation of Bacillus amyloliquefaciens ASAG1

[0023] Through the above identification results, it was confirmed that the strain ASAG1 was Bacillus amyloliquefaciens ( ...

Embodiment 2

[0024] Example 2 The cultivation of Bacillus amyloliquefaciens ASAG1

[0025] (1) Incline culture: culture time 2~3 days, medium composition (1 liter): potato 200 g / L, glucose 20 g / L, agar 20 g / L, agar 15 g, natural pH, culture temperature 28~ 32°C.

[0026] (2) Seed culture: Pick activated single colonies from the slope in the ultra-clean workbench and insert them into the seed culture solution, shake the bacteria at 28°C for 1-2 days at a speed of 200 r / min to obtain the seed solution;

[0027] Seed culture medium composition (1 liter): glucose 20g / L, beef extract 5g / L, magnesium sulfate heptahydrate 1g / L, ammonium sulfate 1g / L, calcium carbonate 3g / L, potassium dihydrogen phosphate 0.6g / L, potato 200g / L, pH 7.0~7.2.

[0028] (3) Fermentation: Add the seed liquid obtained in the above step into the fermentation medium according to the inoculum amount of 5%, and culture it under shaking at 28°C for 2-3 days at a rotation speed of 200 r / min to obtain the fermentation liquid ...

Embodiment 3

[0029] Example 3 Antagonistic experiment of Bacillus amyloliquefaciens ASAG1 on fungi

[0030] The fermentation broth of Bacillus amyloliquefaciens ASAG1 prepared in Example 2 was centrifuged at 12000 r / min for 10 min, and the supernatant was filtered and sterilized by a 0.2 μm sterile microporous membrane, and the inhibition test was carried out by using the Oxford cup quantitative method to measure the pH. The diameter of the bacterial circle was used to determine the antibacterial effect of the strain on 12 common stored grain harmful fungi.

[0031] Among them, Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus, Aspergillus carbon black, Penicillium verruca, Penicillium chrysogenum, and Penicillium chrysogenum were cultured using Cha's medium, and Fusarium tritina, Fusarium seminakedum, and Fusarium oxysporum were cultivated using PDA Base, Aspergillus aeruginosa uses high-salt Chapei medium, and Aspergillus uses concentrated sugar Chapei medium for bacteriostati...

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Abstract

The invention discloses Bacillus amyloliquefaciens and application thereof. The Bacillus amyloliquefaciens ASAG1 is a biocontrol bacterium, wherein the collection number is CGMCC No.3056. The invention also provides a microbial inoculum comprising the collected bacterial strain. The Bacillus amyloliquefaciens or the microbial inoculum has remarkable inhibition activity on stored grain fungi and extinction effect on stored grain mycotoxin.

Description

technical field [0001] The invention relates to a strain of bacillus amyloliquefaciens and its application. Background technique [0002] Grains are the source of energy for people to ingest nutrition and survive, and are the largest and most important material basis in the balanced diet pyramid. The quality deterioration and loss of stored grains caused by mold and pests seriously endanger the safety of my country's stored grains. According to statistics from the Food and Agriculture Organization of the United Nations (FAO), about 25% of food is affected by mycotoxin pollution every year. At present, more than 150 kinds of fungi have been found to endanger grain storage, and more than 200 kinds of mycotoxins can be produced. Human ingestion of these toxins can lead to vomiting, digestive and neurological symptoms, multiple primary cancers, and even death from acute poisoning. Therefore, preventing grain mildew is one of the important methods to maintain grain quality. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L3/3571C12R1/07
Inventor 孙长坡伍松陵吴子丹
Owner ACAD OF STATE GRAIN ADMINISTRATION
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