27 results about "Protozoa" patented technology

The present disclosure provides a method of reducing, preventing or treating an intestinal infection, preventing or treating a disease associated with protozoan parasite of the genus Eimeria, inhibiting an oocyst sporulation of a protozoan parasite or preventing a sporozoite invasion or reproduction, preventing or treating coccidiosis, promoting growth performance, controlling, treating and / or preventing necrotic enteritis in a subject, comprising administering to the subject an effective amount of a composition comprising a Crassocephalum rabens (CR), a bioactive extract thereof (CRE), an active ingredient(s) contained in a CR (CR_API) or CRE (CRE_API), a combination of two or more of a CR, a CRE, a CR_API and a CRE_API, or one or more of a-linolenic acid, citric acid, lactic acid, mannitol, palmitic acid, aspartic acid and 1-monomyristin.

The application discloses a stable anti-reversibility microbial community structure and a construction method and application thereof, and relates to the technical field of microbial community construction, and in particular to a stable anti-reversibility microbial community structure and a construction method and application thereof.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

This invention discloses a strain of Acanthamoeba that inhibits the pathogen of Fusarium graminearum and its applications. Acanthamoeba (Acanthamoeba rhysodes NJAU-ZY1, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: C2025219, demonstrates in plate experiments, liquid co-culture experiments, and pot experiments that inoculation with the protozoan Acanthamoeba NJAU-ZY1 significantly inhibits the growth of Fusarium graminearum pathogen, reduces its infectivity in wheat, alleviates the inhibitory effect of Fusarium graminearum on wheat growth, reduces the risk of diseases caused by this pathogen, and thus increases wheat biomass. This provides a new strategy and resource for the biological control of Fusarium graminearum pathogen and the scientific cultivation of wheat, and has important application value for the safe production of food crops.

A determination has been made that apicomplexan tubulin, an essential parasite component, is an excellent drug target. Here, it is shown that it is possible to specifically target parasite tubulins, without harming the mammalian host. A compound has been identified that destabilizes specifically parasite MTs and inhibits essential parasite processes of replication and host cell invasion. This provides a new strategy to tackle the issue of drug resistance development. Specifically, the use of the compound parabulin and / or its derivates in the process of targeting protozoan tubulin for the inhibition of the replication of protozoans in mammalian organism, wherein parabulin has the following molecular weight, sum formula, name and structure:MW: 373.4 Da;Formula: C20H23NO6, ⋅Name (s): 3-(3,4-dimethoxyphenyl)-N-[(3,4,5-trimethoxyphenyl)methyl]prop-2-enamide; (2E)-3-(3,4-Dimethoxyphenyl)-N-(3,4,5-trimethoxyphenyl)acrylamid;SMILES:0(C1═C(OC)C═CC(═C1)C═CC(═0)N(CC2═CC(═C(OC)C(═C2)OC)OC)[H])C.

This invention provides the application of Usnea powder, Usnea acid, or sodium usneate (or Usnea powder, Usnea acid, or sodium usneate as the sole active ingredient) in the preparation of broad-spectrum antiparasitic drugs for aquatic animals, drugs for treating complications caused by parasitic infections, feed additives, or functional feeds. By utilizing Usnea, Usnea extract, Usnea acid, and Usnea salts, various parasites, including ciliates, worms, and protozoa, that infest the surface and interior of aquatic animals during aquaculture can be eliminated or killed, thus maintaining the health of farmed animals, improving the success rate of aquaculture, achieving broad-spectrum and highly effective prevention and control of aquatic animal parasitic diseases, reducing the use of chemical drugs, and providing good aquaculture, ecological, and social benefits. It is safe, broad-spectrum, and highly effective, facilitating large-scale promotion.

The application belongs to the technical field of microorganism detection, and discloses a method for extracting intracellular bacteria of ameba in a water environment and environmental application; the method comprises the following steps: S1, filtering a water environment sample and ameba enrichment culture; S2, removing extracellular bacteria attached to ameba by coupling treatment of a chelating agent and lysozyme; S3, releasing intracellular bacteria by lysing ameba by using a lysing agent in combination with mechanical suction; and S4, centrifugation and filtration purification to obtain pure intracellular bacteria; the application is simple in operation, low in cost, high in bacterial purity, and can be used for water environment microorganism detection, drinking water safety monitoring, and provides technical support for ameba and intracellular bacteria interaction research.

The invention relates to the field of veterinary protozoology and methods for producing antigens, particularly for the diagnosis of Surra Su-Auru in animals. The objective of the invention is to develop a method for producing a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals. The problem is solved by the fact that the method for obtaining trypanosome antigen for serological diagnostics of Surra (Su-Auru) in animals involves infecting rabbits intratesticularly with live trypanosomes of the T species. evansi, removal of the spleen 2-3 days after infection, total bleeding 8-10 days after splenectomy (parasitemia -80-90%), blood is collected in a wide vessel with 2% sodium citrate in physiological solution with constant stirring with a glass rod, at the rate of 1 part blood to 2 parts solution, settling of citrated blood in the refrigerator for 18-24 hours, centrifugation and separation of the middle layer from the sediment from the supernatant, 2-3 times washing of trypanosomes in physiological solution, at 3000-5000 rpm, for 10 minutes each, disintegration of the centrifugate by ultrasound at a frequency of 22 kHz, power of 60 W / cm2 for 8-12 minutes, followed by centrifugation of the disintegrate at 8000-10000 rpm for 20-30 minutes and collection of sediment (endoantigen), treatment of the supernatant with TCA, collection of the precipitated sediment (exoantigen), batches of sediment are mixed 10:1. The technical result provided by the utility model is expressed in the development of a method for obtaining a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals.

This application relates to the field of microbial technology, and in particular to a method and application for improving soil bioavailable phosphorus using protozoa. This application aims to utilize a soil-environment-friendly protozoan method for improving soil bioavailable phosphorus. The method of this application has advantages such as simple and convenient operation, low cost, environmental friendliness, a rich selection of microbial strains, in-situ application, and avoidance of nutrient loss, low utilization rate, pathogen contamination, and heavy metal contamination caused by excessive fertilization. Simultaneously, it significantly increases the organic phosphorus content of the soil.

The application discloses a high-activity short-cut nitrification fast-starting long-term stable operation process and relates to the technical field of sewage biological treatment, which comprises the following steps: inoculating sludge and performing anaerobic fermentation pretreatment, combining with rapid sludge discharge and manual removal of expanded sludge and sludge foam; controlling sludge age in days, performing sludge repropagation, enriching ammonia-oxidizing bacteria, inhibiting and eluting nitrite-oxidizing bacteria; long-term operation under the condition of no sludge discharge, maintaining the abundance and activity of ammonia-oxidizing bacteria, and simultaneously controlling the abundance of nitrite-oxidizing bacteria; controlling the proliferation of nitrite-oxidizing bacteria by using the predation of protozoa and metazoan, and controlling the nitrite accumulation rate in the reactor; monitoring the concentrations of dissolved oxygen, pH value, ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the reactor in real time, adjusting the ammonia nitrogen load and free ammonia concentration of influent, and adopting intermittent aeration or microporous aeration to adjust the distribution of dissolved oxygen and optimize the operation conditions of the short-cut nitrification reactor. The application is simple, economical and effective.

The present application relates to an anticoccidial composition comprising coumaric acid and use thereof. A composition comprising coumaric acid and / or a salt thereof, according to an embodiment, has excellent effects of inhibiting the cell penetration of protozoa that can induce coccidiosis and / or inhibiting the proliferation of the protozoa in cells, has excellent effects of preventing, ameliorating and treating coccidiosis in vivo, and remarkably reduces oocyst excretion in feces, thus being able to reduce secondary coccidiosis infection.

Provided herein is a method for breeding cephalopod larvae in a rearing tank having a water changing means. The method includes a water-changing period during which the ratio of water changed by the water-changing means per day is 200% or more relative to the quantity of water in the rearing tank in a floating breeding period of the cephalopod larvae.

The present application relates to an anticoccidial composition comprising a stilbene-based compound, and a use thereof. A composition comprising a compound or a salt thereof, according to one embodiment, has excellent effects of direct killing of protozoa that can induce coccidiosis, inhibiting the cell penetration of the protozoa and / or inhibiting the intracellular proliferation of the protozoa, and preventing, alleviating, and treating in vivo coccidiosis.

The application discloses a protozoan filosea and application of the protozoan filosea in promoting growth of tomatoes and preventing and controlling tomato wilt. Gymnophrys cometa ) NJAU-S1, which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: C2025398. Potted test results show that inoculation of the protozoan filosea NJAU-S1 can significantly promote growth of tomatoes, and plate test results show that inoculation of the protozoan filosea NJAU-S1 can inhibit growth of fusarium oxysporum f. sp. lycopersici and reduce the infection ability of the fusarium oxysporum f. sp. lycopersici to tomatoes, thereby relieving the inhibition of the fusarium oxysporum f. sp. lycopersici on the growth of the tomatoes. The application provides a new strategy and resource for biological prevention and control of the fusarium oxysporum f. sp. lycopersici and scientific planting of the tomatoes, and has important application value for safe production of food crops.

This invention relates to a method for cultivating cyanobacteria to control protozoa, comprising the following steps: culturing cyanobacteria to the logarithmic growth phase to obtain a first microalgal culture; encapsulating the first microalgal culture in a polymer to obtain microalgal beads; inoculating the microalgal beads into a liquid culture medium for production cultivation to obtain a second microalgal culture; and when the second microalgal culture is contaminated by protozoa, separating the microalgal beads from the second culture and inoculating them into a liquid culture medium for production cultivation. This invention uses sodium alginate to encapsulate microalgae to control biocontamination, and the method also provides significant process advantages. Under static conditions, the rapid settling of the microalgal beads greatly simplifies biomass harvesting, eliminating the energy-intensive centrifugation step typically required for free cyanobacterial cells. This physical protection platform provides a scalable and environmentally sustainable solution to the persistent challenge of microbial contamination in large-scale cyanobacterial biofuel production.

The application discloses an extractable nucleic acid trichomonad activity protection solution and relates to the technical field of biotechnology, which comprises two component reagents A and B, the reagent A comprises 0.8-1.0% NaCl, 0.1-5% Tris-Cl buffer, 0.1-1.5% SDS, 1.5-5% glucose, 5-15% serum, 0.5-5% betaine, and the balance is sterile deionized water, and the reagent B is a proteinase K solution. The application can not only dilute secretion, preserve trichomonas and culture trichomonas, but also be used for extracting trichomonad nucleic acid in PCR molecular experiment, so that the application can realize double application of trichomonad detection. The application not only helps to solve the health problems of women and improve fertility protection, but also is the first domestic invention to provide an example for new technology transformation service to the society.

The invention discloses a strain of protocyst violaceum and application of the protocyst violaceum in biological prevention and treatment of wheat stem rot, and the strain WE12 has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.36395 and is classified and named as the protocyst violaceum. The protocyst purple WE12 disclosed by the invention has efficient predatory activity on various pathogenic bacteria causing wheat stem rot, and meanwhile, degerming fermentation filtrate and volatile metabolites of the strain also show remarkable bacteriostatic activity on the wheat stem rot, so that the protocyst purple WE12 has multiple antibacterial mechanisms. Indoor potting and field control effect tests show that the protocyst purpureum WE12 not only has a good control effect on wheat stem rot caused by single pathogenic bacteria, but also has a good control effect on compound infection of multiple pathogens, and also has remarkable growth promoting and yield increasing effects. The application potential in the aspect of biological prevention and control of the wheat stem rot is huge.

Described herein are compositions and methods for the treatment or prevention of dermal or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., demodex), worms, protozoa (e.g., amoeba (e.g., acanthamoeba or cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. Said compositions and methods comprise compounds which demonstrate immunological, keratolytic, anti-histamine, anti-inflammatory, and / or other desirable activities. Topical administration of said compositions to the eyelid margin or surrounding areas provides therapeutic benefit to patients suffering from ocular surface disorders.

The present invention relates to a stable hydrogen peroxide in the form of 10 ‑4 -10 3 nanometer diameter coated particles with one or more antimicrobial plant extracts. These plant extracts are particularly stabilized with one or more non-toxic alcohols, such as polyols having hydrogen- absorbing properties. The stabilized hydrogen peroxide according to the present invention can be used as a disinfectant in a wide range of applications by removing bacteria, fungi, viruses, algae, protozoa and / or nematodes. For example, disinfection of surfaces, equipment, air, water and applications in human hygiene can be envisaged here, in short, any application for which hydrogen peroxide is suitable.

A composition comprising the dry extract of ruminal content of bovine and sheep and the caecal content of rabbit is described which can be used as a powdered dietary supplement for pets and farm animals as it retains intact the nutritional components of the starting material, such as volatile fatty acids, amino acids, vitamins, minerals and dietary fibres, but it also contains a very high load of bacterial derivatives, protozoa and yeasts, which are able to stimulate the immune system of the gastrointestinal tract; and the process for preparing it.

The utility model discloses a kind of plankton shallow water area surface layer collecting devices, it is related to aquatic biological resource investigation technical field, including collection frame, one end of the collection frame is provided with connecting frame, the one end of the connecting frame away from collection frame is provided with driving device, two rhombic floating plates one are fixedly connected with the two sides of the collection frame symmetry, two rhombic floating plates two are fixedly connected with the two sides of the driving device symmetry, the rhombic floating plate one and rhombic floating plate two are aligned in device longitudinal outside end face, the rhombic floating plate one and rhombic floating plate two are used to prevent plankton collection device from rolling over. The utility model is through nylon screen surface and silk cloth screen surface, the layered filtering mechanism of double-layer filter screen, through 200 μm nylon screen surface intercepts large plankton and floating object, avoids its interference micro sample. Through 50 μm silk cloth screen surface accurate retention small phytoplankton and protozoa.

A particulate oxide composition comprising cerium oxide, trivalent dopant, and optional additional metal oxide, other than cerium oxide and trivalent dopant, is beneficial to aid in the removal of biological contaminants, such as bacteria, viruses, fungi, protozoa (e.g., amoebae), yeast and algae. This particulate oxide composition contains more cerium oxide than trivalent dopant and has a unique depth profile in which the average trivalent dopant to Ce ratio at about 0 nm to about 3.5 nm from the surface of the particulate composition is greater than the trivalent dopant to Ce ratio at about 15 nm from the surface of the particulate composition. These trivalent doped cerium oxide compositions can be used to remove these biological contaminants from fluids, including air and water, and from solid surfaces. Also described are methods of using compositions containing these trivalent doped cerium oxide compositions to remove biological contaminants.

The double biofilm sewage treatment process for deep denitrification and dephosphorization coupled with sludge reduction belongs to the technical field of sewage treatment. The process is composed of an anoxic biofilm reaction tank, an aerobic biofilm reaction tank and a ferric autotrophic denitrification biofilter, and high-efficiency denitrification and dephosphorization of sewage is achieved through the double biofilm sewage treatment process. In the front-end biofilm reaction tank, denitrification and dephosphorization and in-situ sludge reduction are mainly carried out, and in the ferric autotrophic denitrification biofilter, deep denitrification and dephosphorization are mainly carried out. The protozoa and metazoan food chain of the aerobic biofilm reaction tank is used to prey on the residual sludge, achieving the effect of zero residual sludge production. By adjusting the reflux ratio of the ferric ion-containing reflux liquid generated by the ferric autotrophic denitrification biofilter to change the concentration of ferric ions, the number and community structure of microorganisms are effectively controlled, and the control of sludge predation is achieved, improving the sludge reduction effect. The process has obvious effect, can be operated stably for a long time, and is suitable for engineering application.

The application provides a compound TU-BDN, a preparation method thereof and application of the compound in preparation of a medicine for preventing or treating toxoplasma infection. 20 H 14 N4O5S, the application discloses that TU-BDN has significant inhibitory activity on intracellular parasitic protozoa toxoplasma, has small toxic side effects, and can expand a new way for effective use of TU-BDN. Vero cells infected with toxoplasma are used as an in-vitro experimental model to verify the anti-toxoplasma activity and cell safety of the compound TU-BDN, and the results show that the half effective inhibitory concentration of TU-BDN on toxoplasma is 3.231 micromoles, TU-BDN can significantly inhibit the proliferation of toxoplasma in host cells, the half cytotoxic concentration of TU-BDN on Vero cells is 105.8 micromoles, and the safety index SI is 32.75. Compared with the existing medicines for treating toxoplasma infection, TU-BDN has the problems of large side effects and poor effect, the effective inhibitory concentration of TU-BDN is much smaller than the cytotoxicity, TU-BDN has significant effects on prevention and treatment of toxoplasma infection, and TU-BDN can be widely used for preparation of a medicine for preventing or treating toxoplasma infection.

The present disclosure relates to methods of treating infections caused by one or more protozoa such as Cryptosporidium, Eimeria, Babesia and Toxoplasma comprising administering to a subject in need thereof an effective amount of an inhibitor of plasmepsin IX and / or X selected from compounds disclosed herein.