74 results about "Protozoa" patented technology

The invention belongs to the technical field of sewage treatment, and discloses a DO-ORP coordinated regulation artificial tidal food web sludge in-situ reduction and phosphorus recovery method. Aiming at the problems of high energy consumption, serious phosphorus resource loss and the like of traditional sludge treatment, a multi-stage food chain consisting of microorganisms, protozoa and metazoan is implanted by constructing an artificial tidal environment with periodic alternation of wetting and drying; according to the method, the metabolic activity of sludge hydrolysis flora and phosphorus-accumulating bacteria is activated by utilizing cooperative regulation and control of DO concentration and ORP, microbial extracellular polymeric substances are triggered to be depolymerized by adopting tidal periodic oscillation, in-situ reduction of sludge biomass is realized by combining a food web cascade predation effect, and phosphorus is synchronously removed by virtue of an anaerobic phosphorus release-aerobic phosphorus uptake coupled struvite crystallization process. The method is suitable for treating the excess sludge of a municipal sewage treatment plant, and the high-purity magnesium ammonium phosphate fertilizer is produced while the dehydration energy consumption is reduced by 40%.

The present disclosure provides a biological control method for ciliate disease, and belongs to the technical field of biological control. The biological control method includes the following step: adding Chaenea vorax into an aquaculture pond to control the ciliate disease; and the Chaenea vorax includes Chaenea vorax PJ13002. According to the present disclosure, the Chaenea vorax PJ13002, a predatory protozoan, is introduced to recover a microbial food chain existing in nature in an aquaculture system and achieve biological control of the ciliate disease. Meanwhile, the Chaenea vorax PJ13002 can be directly ingested by aquaculture animals without causing diseases, so that a more stable aquaculture system can be formed, losses are avoided, costs are reduced, and economic benefits are increased. The method provides a new thought for controlling the ciliate disease and has an excellent popularization and application prospect.

Described herein are compositions and methods for treating or preventing skin or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., Demodex), helminths, protozoa (e.g., amebas (e.g., Acanthamoeba or Cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. The compositions and methods include compounds that exhibit immunological activity, keratolytic activity, antihistamine activity, anti-inflammatory activity, and / or other desirable activities. Topical administration of the compositions to the eyelid margin or surrounding area provides therapeutic benefit to patients with ocular surface disorders.

In one aspect, the disclosure relates to immunogenic compositions comprising a semisynthetic saponin adjuvant (VSA) including, but not limited to, VSA-1 and VSA-2, derived from a Momordica saponin and at least one antigen. In a further aspect, the immunogenic compositions can include other known adjuvants, emulsifiers, and / or surface-active agents and can be formulated as liposomes, micelles, emulsions, and the like. Also disclosed are methods of eliciting effective immune responses to viral, bacterial, protozoal, and neoplastic diseases using the disclosed immunogenic compositions.

The present disclosure provides a method of reducing, preventing or treating an intestinal infection, preventing or treating a disease associated with protozoan parasite of the genus Eimeria, inhibiting an oocyst sporulation of a protozoan parasite or preventing a sporozoite invasion or reproduction, preventing or treating coccidiosis, promoting growth performance, controlling, treating and / or preventing necrotic enteritis in a subject, comprising administering to the subject an effective amount of a composition comprising a Crassocephalum rabens (CR), a bioactive extract thereof (CRE), an active ingredient(s) contained in a CR (CR_API) or CRE (CRE_API), a combination of two or more of a CR, a CRE, a CR_API and a CRE_API, or one or more of a-linolenic acid, citric acid, lactic acid, mannitol, palmitic acid, aspartic acid and 1-monomyristin.

The invention belongs to the technical field of biology, and particularly relates to a method for increasing the fucoxanthine accumulation amount by using microcystis subjected to depolymerization treatment to feed palmarthria formosana. According to the method, microcystis subjected to depolymerization treatment are added in the culture process of the palmitothecium for heterotrophic culture, meanwhile, continuous inflation is conducted, the microcystis subjected to depolymerization treatment are swallowed by the palmitothecium, and the higher fucoxanthine accumulation amount in the palmitothecium is obtained by means of the culture mode. The experiment proves that after the microcystis is subjected to pretreatment and depolymerization, the removal efficiency of the microcystis is higher by the brown algae, the limitation of treatment of harmful cyanobacteria and ingestion of protozoa is made up, and meanwhile, an integrated solution is provided for biological treatment of cyanobacterial bloom and resource utilization of the microalgae; meanwhile, a new way is provided for obtaining fucoxanthine with higher accumulation amount.

The application discloses a method for preserving and recovering hydrozoa and medusae, and the method is characterized by the following steps: inducing the hydrozoa to degenerate and enter a dormant state by high salinity, and recovering the hydrozoa from the dormant state in 3 days at the fastest speed when the salinity is restored to 30-35 ppt, and differentiating medusae buds in one week. The method is suitable for preserving and recovering various hydrozoa such as dactylozooid hand medusae, Xiamen anthoathecate medusae and high hand medusae. The method not only solves the problems of long-term preservation and population rejuvenation of hydrozoa, but also effectively removes algae, protozoa, nematodes and other organisms, speeds up the formation and development of medusae buds, greatly saves the manpower and material resources for long-term maintenance of hydrozoa, and improves the efficiency of long-term preservation and maintenance of hydrozoa.

The present invention relates to compositions comprising an inactivated Mycobacterium or an immunogenic fraction thereof, for use in the prevention of an infection in a subject, wherein the infection is selected from the group consisting of a protozoan parasitic infection and a bacterial infection, with the proviso that the infection is not caused by a Mycobacterium.

The application discloses a stable anti-reversibility microbial community structure and a construction method and application thereof, and relates to the technical field of microbial community construction, and in particular to a stable anti-reversibility microbial community structure and a construction method and application thereof.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

Cryptosporidium parvum is a highly prevalent zoonotic and anthroponotic protozoan parasite that causes a diarrheal syndrome in children and neonatal livestock, culminating in growth retardation and mortalities. Disclosed herein are inhibitors against the enzymatic activity of recombinant CpLDH protein that were identified. The inhibitors were tested for anti-Cryptosporidium effect using in vitro infection assays of HCT-8 cells monolayers. Compounds NSC158011 and NSC10447 were identified to inhibit the proliferation of intracellular C. parvum in vitro, with IC50 values of 14.88 and 72.65 μM, respectively. At doses tolerable in mice, both NSC158011 and NSC10447 significantly reduced the shedding of C. parvum oocysts in infected immunocompromised mice's feces and prevented intestinal villous atrophy as well as mucosal erosion due to C. parvum. These findings have unveiled anti-Cryptosporidium drug candidates that can be explored further for the development of therapeutic agents against C. parvum infections.

The invention discloses protozoa zopoda and application of the protozoa zopoda in promotion of tomato growth and prevention and control of fusarium wilt. The protozoa zopoda cometa NJAU-S1 is preserved in the China Center for Type Culture Collection, and the preservation number of the protozoa zopoda cometa NJAU-S1 is CCTCC (China Center for Type Culture Collection) NO: C2025398. Pot experiment results show that inoculation of protozoa nematopoda NJAU-S1 can significantly promote tomato growth, and plate experiments show that inoculation of protozoa nematopoda NJAU-S1 can inhibit growth of tomato fusarium oxysporum and reduce the infection ability of tomato fusarium oxysporum to tomato, so that the inhibition effect of tomato fusarium oxysporum on tomato growth is relieved. The invention provides a new strategy and resource for biological prevention and control of tomato fusarium oxysporum and scientific planting of tomatoes, and has important application value for safe production of food crops.

The present application belongs to the technical field of drinking water disinfection, and particularly relates to a method for inactivating ameba and endogenous organisms thereof by electrocatalytic water oxidation. In order to develop a feasible technology for effectively inactivating ameba and endogenous organisms in spores thereof, the present application provides an electrocatalytic water oxidation method for inactivating ameba and endogenous organisms thereof based on advanced oxidation technology. The method can generate strong oxidizing substances such as hydrogen peroxide and hydroxyl radicals in situ, and then inactivate ameba and endogenous bacteria in spores thereof, and the inactivation effect is excellent. Meanwhile, the method has the advantages of simple equipment, convenient operation, low cost, environmental friendliness and the like, and provides a feasible electrocatalytic water oxidation technology for effectively inactivating ameba and endogenous organisms in spores thereof.

This invention discloses a strain of Acanthamoeba that inhibits the pathogen of Fusarium graminearum and its applications. Acanthamoeba (Acanthamoeba rhysodes NJAU-ZY1, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: C2025219, demonstrates in plate experiments, liquid co-culture experiments, and pot experiments that inoculation with the protozoan Acanthamoeba NJAU-ZY1 significantly inhibits the growth of Fusarium graminearum pathogen, reduces its infectivity in wheat, alleviates the inhibitory effect of Fusarium graminearum on wheat growth, reduces the risk of diseases caused by this pathogen, and thus increases wheat biomass. This provides a new strategy and resource for the biological control of Fusarium graminearum pathogen and the scientific cultivation of wheat, and has important application value for the safe production of food crops.

The invention provides a method for removing protozoa in a chlorella species conservation bottle, which adopts an algae culture tool and seawater to disinfect, and is characterized in that: sodium hypochlorite, bleaching powder or bleaching powder concentrate and other disinfectants containing available chlorine are added to ensure that the concentration of available chlorine in algae reaches 5mg / L. Then, standing the water body for 1 hour so as to settle protozoa to the water bottom; separating the algae liquid on the upper layer into a new container, adding sodium thiosulfate with the addition amount being 1 / 10 of the available chlorine amount in the algae liquid, and neutralizing the residual available chlorine by using the sodium thiosulfate according to the concentration of 0.5 mg / L; after 48 hours of primary treatment, adding sodium hypochlorite, bleaching powder or bleaching powder concentrate and other disinfectants containing available chlorine again for disinfection, continuing to separate the algae liquid, and adding sodium thiosulfate with corresponding concentration again for neutralization; and the protozoa in the breed conservation bottle can be effectively removed after the second treatment.

A determination has been made that apicomplexan tubulin, an essential parasite component, is an excellent drug target. Here, it is shown that it is possible to specifically target parasite tubulins, without harming the mammalian host. A compound has been identified that destabilizes specifically parasite MTs and inhibits essential parasite processes of replication and host cell invasion. This provides a new strategy to tackle the issue of drug resistance development. Specifically, the use of the compound parabulin and / or its derivates in the process of targeting protozoan tubulin for the inhibition of the replication of protozoans in mammalian organism, wherein parabulin has the following molecular weight, sum formula, name and structure:MW: 373.4 Da;Formula: C20H23NO6, ⋅Name (s): 3-(3,4-dimethoxyphenyl)-N-[(3,4,5-trimethoxyphenyl)methyl]prop-2-enamide; (2E)-3-(3,4-Dimethoxyphenyl)-N-(3,4,5-trimethoxyphenyl)acrylamid;SMILES:0(C1═C(OC)C═CC(═C1)C═CC(═0)N(CC2═CC(═C(OC)C(═C2)OC)OC)[H])C.

The invention relates to the cross technical field of molecular biology and parasitology, and particularly discloses a giardia living body marking method based on fluorescent protein UnaG, which comprises the following steps: firstly, optimizing and synthesizing a UnaG gene sequence according to the codon use preference of giardia; then, constructing a marker plasmid pGT-UnaG-neo on a double expression framework containing an alpha2-tubulin promoter and a gdh promoter, and introducing the plasmid into the giardia giardia trophozoite through electroporation. And carrying out resistance screening in a culture medium containing geneticin to obtain an insect strain capable of stably integrating and expressing UnaG. Through bilirubin induction, efficient maturation and green fluorescence emission of the UnaG protein in giardia cytoplasm are realized. The stably transferred strain obtained by the method has no significant difference from a wild strain in the aspects of growth curve and survival rate, and UnaG and bilirubin have no obvious toxic effect. The limitation that traditional fluorescent protein is inactivated under the anaerobic condition is broken through, and a reliable tool is provided for dynamic imaging, functional research and drug screening of giardia and other anaerobic protozoa.

Disclosed are advantageous systems and methods for treating building water systems, especially the interior surfaces of premise plumbing, to remove biofilm and inactivate biofilm-associated pathogens, including protozoa, using disinfectant formulations at concentrations at in excess of those used for drinking water treatment, and further, in co-applying complexing agents to mitigate corrosion of the materials treated; and using these in conjunction with off-gas containment devices that allow flushing of taps without the liberation of toxic fumes.

The invention provides a protozoa long-term preservation method, and belongs to the technical field of protozoa germplasm preservation. The invention provides a protozoa long-term preservation method. Sterilized nutrient soil is used as a protozoa preservation medium. The method for long-term preservation of protozoa in a simulated nutrient soil environment is created for the first time, the simulated microenvironment is constructed on the basis of sterilized nutrient soil according to the natural inhabiting characteristic of protozoa, and the limitation that a nutrient soil pore structure and a nutrient slow release mechanism cannot be simulated through traditional liquid culture or filter membrane breed conservation is broken through; the protozoa polypide is subjected to a life history in a similar original habitat, long-period preservation without intermediate operation is realized, and the problems of short preservation time, complex operation, passage pollution, low recovery rate, inconvenient transportation and the like in traditional breed conservation are effectively solved.

This invention provides the application of Usnea powder, Usnea acid, or sodium usneate (or Usnea powder, Usnea acid, or sodium usneate as the sole active ingredient) in the preparation of broad-spectrum antiparasitic drugs for aquatic animals, drugs for treating complications caused by parasitic infections, feed additives, or functional feeds. By utilizing Usnea, Usnea extract, Usnea acid, and Usnea salts, various parasites, including ciliates, worms, and protozoa, that infest the surface and interior of aquatic animals during aquaculture can be eliminated or killed, thus maintaining the health of farmed animals, improving the success rate of aquaculture, achieving broad-spectrum and highly effective prevention and control of aquatic animal parasitic diseases, reducing the use of chemical drugs, and providing good aquaculture, ecological, and social benefits. It is safe, broad-spectrum, and highly effective, facilitating large-scale promotion.

The present application belongs to the technical field of large-scale seed cultivation of shellfish, and discloses a large-scale seed cultivation method of Patella pellucida. According to the practical breeding experience, it is found that the main reasons for the failure of seed cultivation are high mortality in the plankton and attachment stages, the vulnerability of the water body to the invasion of protozoa, the easy outbreak of harmful algae in the water body and the like, which lead to the failure of the development of the juvenile stage to the benthic life. The present application successfully breaks through the bottleneck of the seed cultivation of Patella pellucida by optimizing the seed cultivation environment, improving the seed cultivation technology and controlling the light, and can cultivate the Patella pellucida seed on a large scale. The juvenile snails with the size of 1-2 cm, which are suitable for proliferation, release or artificial cultivation, are provided, and the present application provides a solid reference for the breeding of Patella pellucida.

The application belongs to the technical field of microorganism detection, and discloses a method for extracting intracellular bacteria of ameba in a water environment and environmental application; the method comprises the following steps: S1, filtering a water environment sample and ameba enrichment culture; S2, removing extracellular bacteria attached to ameba by coupling treatment of a chelating agent and lysozyme; S3, releasing intracellular bacteria by lysing ameba by using a lysing agent in combination with mechanical suction; and S4, centrifugation and filtration purification to obtain pure intracellular bacteria; the application is simple in operation, low in cost, high in bacterial purity, and can be used for water environment microorganism detection, drinking water safety monitoring, and provides technical support for ameba and intracellular bacteria interaction research.

Avian coccidiosis is a disease caused by an intracellular parasitic protozoan of the genus Eimeria, which induces lesions in the gastrointestinal tissues of birds due to its replication. The process of qualifying an oocyst is almost entirely manual, using, in particular, the optical microscope counting technique, in which each identified object must be classified as sporulated or non-sporulated, stained or unstained. For the identification process, the method used is PCR (polymerase chain reaction), in which the presence of each species is detected, but without identifying each oocyst. This manual process is time-consuming and susceptible to errors. The method proposed herein introduces a computer vision model, based on Artificial Intelligence, capable of automating this classification process. The model analyzes samples and provides quantitative results, including the classification of oocysts into the categories of sporulated, non-sporulated, stained, and unstained, as well as the identification of Eimeria species (acervulina, brunetti, maxima, mitis, necatrix, praecox, or tenella). This computational model is entirely based on machine learning and is capable of identifying the presence of oocysts in a sample (image), classifying each identified oocyst into categories such as sporulated or non-sporulated, stained or unstained, quantifying the objects in each category, and identifying the Eimeria species.

The invention discloses an acanthamoeba protozoa for inhibiting fusarium graminearum pathogenic bacteria and application of the acanthamoeba protozoa. The acanthamoeba rhysodes NJAU-ZY1 is preserved in the China Center for Type Culture Collection, and the preservation number of the acanthamoeba rhysodes NJAU-ZY1 is CCTCC (China Center for Type Culture Collection) NO: C2025219. Results of a plate experiment, a liquid co-culture experiment and a pot experiment show that inoculation of the protozoa acanthamoeba NJAU-ZY1 can significantly inhibit the growth of the fusarium graminearum pathogenic bacteria and reduce the infection ability of the fusarium graminearum pathogenic bacteria on wheat, so that the inhibition effect of the fusarium graminearum pathogenic bacteria on the growth of the wheat is relieved, and the growth of the wheat is inhibited. The disease risk caused by the pathogenic bacteria is reduced, so that the wheat biomass is improved, a new strategy and a new resource are provided for biological prevention and control of the fusarium graminearum pathogenic bacteria and scientific wheat planting, and the method has an important application value for safe production of food crops.

The invention relates to the field of veterinary protozoology and methods for producing antigens, particularly for the diagnosis of Surra Su-Auru in animals. The objective of the invention is to develop a method for producing a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals. The problem is solved by the fact that the method for obtaining trypanosome antigen for serological diagnostics of Surra (Su-Auru) in animals involves infecting rabbits intratesticularly with live trypanosomes of the T species. evansi, removal of the spleen 2-3 days after infection, total bleeding 8-10 days after splenectomy (parasitemia -80-90%), blood is collected in a wide vessel with 2% sodium citrate in physiological solution with constant stirring with a glass rod, at the rate of 1 part blood to 2 parts solution, settling of citrated blood in the refrigerator for 18-24 hours, centrifugation and separation of the middle layer from the sediment from the supernatant, 2-3 times washing of trypanosomes in physiological solution, at 3000-5000 rpm, for 10 minutes each, disintegration of the centrifugate by ultrasound at a frequency of 22 kHz, power of 60 W / cm2 for 8-12 minutes, followed by centrifugation of the disintegrate at 8000-10000 rpm for 20-30 minutes and collection of sediment (endoantigen), treatment of the supernatant with TCA, collection of the precipitated sediment (exoantigen), batches of sediment are mixed 10:1. The technical result provided by the utility model is expressed in the development of a method for obtaining a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals.

The invention relates to methods of inhibiting or treating neurological damage in a human who is infected by or at risk of infection by a virus, a bacterium, a fungus, a protozoan or a parasite that can cause neurological damage, comprising administering to a human a compound selected from the group consisting of Formula (I), Formula (II), Formula (III) or Formula (IV) or pharmaceutically acceptable salts thereof.

The present application relates to the use of a composition for preventing, alleviating or treating coccidiosis and against Eimeria protozoa. The composition comprises an active ingredient for preventing, relieving or treating coccidiosis, and the active ingredient consists of coumaric acid or a pharmaceutically acceptable salt thereof. According to one embodiment, the composition comprising cumaric acid and / or a salt thereof has an excellent effect of inhibiting cell infiltration of protozoa capable of inducing coccidiosis and / or inhibiting proliferation of protozoa in cells, has an excellent effect of preventing, alleviating and treating coccidiosis in vivo, and significantly reduces the oocyst excretion rate in faeces, and thus has an excellent effect of preventing, alleviating and treating coccidiosis in vivo. Therefore, secondary coccidiosis infection can be reduced.

The present invention relates to metagenomics methods. The invention also relates to their use in the detection and / or diagnosis of a wide range of infectious diseases, for example, caused by a pathogenic virus, bacterium, fungus or protozoan. The invention also extends to methods for sample preparation and microorganism detection and / or identification, and methods for host cell nucleic acid depletion. The invention further relates to kits and apparatus used in these methods. The invention is especially useful in clinical diagnostic and veterinary medicine.

Described herein are compositions and methods for the treatment or prevention of dermal or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., demodex), worms, protozoa (e.g., amoeba (e.g., acanthamoeba or cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. Said compositions and methods comprise compounds which demonstrate immunological, keratolytic, anti-histamine, anti-inflammatory, and / or other desirable activities. Topical administration of said compositions to the eyelid margin or surrounding areas provides therapeutic benefit to patients suffering from ocular surface disorders.