109 results about "Protozoa" patented technology

The invention discloses a high-activity short-cut nitrification quick-start long-term stable operation process, which relates to the technical field of biological sewage treatment, and comprises the following steps: inoculating sludge, carrying out anaerobic fermentation pretreatment, and removing expanded sludge and sludge foam in combination with quick sludge discharge and manual removal; controlling the number of days of sludge age, reproliferating the sludge, enriching ammonia oxidizing bacteria, and inhibiting and elutriating nitrite oxidizing bacteria; long-term operation is carried out under the condition of no sludge discharge, the abundance and activity of ammonia oxidizing bacteria are maintained, and meanwhile, the abundance of nitrite oxidizing bacteria is controlled; the proliferation of nitrite oxidizing bacteria is controlled by using the predation effect of protozoa and metazoan, and the accumulation rate of nitrite in the reactor is controlled; monitoring the dissolved oxygen, the pH value and the concentrations of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen in the reactor in real time, and regulating and controlling the influent ammonia nitrogen load and the free ammonia concentration; dissolved oxygen distribution is adjusted in an intermittent aeration or microporous aeration mode, and the operation conditions of the short-cut nitrification reactor are optimized. The method is simple to operate, economical and effective.

The invention relates to the technical field of biology, in particular to a sample preservation solution for vagina micro-ecology detection and a preparation method of the sample preservation solution. The invention relates to a sample preservation solution for vaginal micro-ecology detection. The sample preservation solution comprises the following components in percentage by mass: 0.5-0.9% of paraformaldehyde, 0.08-0.12% of glutaraldehyde, 10-18% of a buffer solution, 0.1-0.4% of inositol, 1-10% of a stabilizer, 0.8-5% of a cell treatment agent and the balance of water, the stabilizing agent is prepared from sodium alginate, tween-20 and beta-cyclodextrin. The invention aims to solve the problems of high false negative and false positive rate or difficulty in interpretation caused by liquid-based cell accumulation, unstable subsequent dyeing and decoloration time and the like of a cell preservation solution in the prior art, so that the sample preservation solution for sample cells can keep the forms of cells, protozoa and the like unchanged, and ensures that gram staining negative and positive properties of bacteria and fungi are not changed. The staining and decoloring are relatively stable, the false negative and false positive rate of sample cell detection is effectively reduced, and the sample cell lesion detection and judgment are more accurate.

A particulate oxide composition comprising cerium oxide, trivalent dopant, and optional additional metal oxide, other than cerium oxide and trivalent dopant, is beneficial to aid in the removal of biological contaminants, such as bacteria, viruses, fungi, protozoa (e.g., amoebae), yeast and algae. This particulate oxide composition contains more cerium oxide than trivalent dopant and has a unique depth profile in which the average trivalent dopant to Ce ratio at about 0 nm to about 3.5 nm from the surface of the particulate composition is greater than the trivalent dopant to Ce ratio at about 15 nm from the surface of the particulate composition. These trivalent doped cerium oxide compositions can be used to remove these biological contaminants from fluids, including air and water, and from solid surfaces. Also described are methods of using compositions containing these trivalent doped cerium oxide compositions to remove biological contaminants.

Agrochemical compositions including an agriculturally suitable carrier; one or more encysted or sporulated protozoa (protist); and an agricultural payload including an agrochemical with or without a food source for protozoa such as a agriculturally nonbeneficial bacteria are provided. Also provided is a modified plant seed, and methods for improving plant growth, reducing plant loss to pests, and controlling or eliminating pest infestation of a crop plant, each including use of the described agrochemical compositions.

The invention provides a method for inhibiting soil acidification and application of protozoa to preparation of biological fertilizer.The method comprises the step that the biological fertilizer containing the protozoa is inoculated into soil, and the protozoa comprises at least one of kidney-shaped worms and ochramydae. By the adoption of the technical scheme, the purpose of rapidly inhibiting soil acidification is achieved, the increase of the organic carbon content of soil can be remarkably promoted, meanwhile, different types of protozoa have different degrees of effects on key nutrient elements such as solid storage nitrogen and phosphorus, and a soil nutrient supply structure is optimized. In the aspect of improving the physical structure of the soil, the life activity of the protozoa can effectively promote the generation of soil aggregates, and the healthy soil structure is remodeled by improving the porosity and air permeability of the soil. In addition, the protozoa can also form a complex interaction network with microorganisms such as bacteria and fungi in soil, so that a microbial metabolic pathway is activated, and the microbial activity is remarkably improved.

The invention belongs to the technical field of sewage treatment, and discloses a DO-ORP coordinated regulation artificial tidal food web sludge in-situ reduction and phosphorus recovery method. Aiming at the problems of high energy consumption, serious phosphorus resource loss and the like of traditional sludge treatment, a multi-stage food chain consisting of microorganisms, protozoa and metazoan is implanted by constructing an artificial tidal environment with periodic alternation of wetting and drying; according to the method, the metabolic activity of sludge hydrolysis flora and phosphorus-accumulating bacteria is activated by utilizing cooperative regulation and control of DO concentration and ORP, microbial extracellular polymeric substances are triggered to be depolymerized by adopting tidal periodic oscillation, in-situ reduction of sludge biomass is realized by combining a food web cascade predation effect, and phosphorus is synchronously removed by virtue of an anaerobic phosphorus release-aerobic phosphorus uptake coupled struvite crystallization process. The method is suitable for treating the excess sludge of a municipal sewage treatment plant, and the high-purity magnesium ammonium phosphate fertilizer is produced while the dehydration energy consumption is reduced by 40%.

The present disclosure provides a biological control method for ciliate disease, and belongs to the technical field of biological control. The biological control method includes the following step: adding Chaenea vorax into an aquaculture pond to control the ciliate disease; and the Chaenea vorax includes Chaenea vorax PJ13002. According to the present disclosure, the Chaenea vorax PJ13002, a predatory protozoan, is introduced to recover a microbial food chain existing in nature in an aquaculture system and achieve biological control of the ciliate disease. Meanwhile, the Chaenea vorax PJ13002 can be directly ingested by aquaculture animals without causing diseases, so that a more stable aquaculture system can be formed, losses are avoided, costs are reduced, and economic benefits are increased. The method provides a new thought for controlling the ciliate disease and has an excellent popularization and application prospect.

An adjuvant composition is disclosed by the present disclosure. The adjuvant composition comprises: a saponin conjugate represented by formula (I) or a pharmaceutically acceptable salt or solvate thereof; and a TLR agonist, wherein the formula (I) is shown as follows:

Described herein are compositions and methods for treating or preventing skin or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., Demodex), helminths, protozoa (e.g., amebas (e.g., Acanthamoeba or Cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. The compositions and methods include compounds that exhibit immunological activity, keratolytic activity, antihistamine activity, anti-inflammatory activity, and / or other desirable activities. Topical administration of the compositions to the eyelid margin or surrounding area provides therapeutic benefit to patients with ocular surface disorders.

In one aspect, the disclosure relates to immunogenic compositions comprising a semisynthetic saponin adjuvant (VSA) including, but not limited to, VSA-1 and VSA-2, derived from a Momordica saponin and at least one antigen. In a further aspect, the immunogenic compositions can include other known adjuvants, emulsifiers, and / or surface-active agents and can be formulated as liposomes, micelles, emulsions, and the like. Also disclosed are methods of eliciting effective immune responses to viral, bacterial, protozoal, and neoplastic diseases using the disclosed immunogenic compositions.

The present disclosure provides a method of reducing, preventing or treating an intestinal infection, preventing or treating a disease associated with protozoan parasite of the genus Eimeria, inhibiting an oocyst sporulation of a protozoan parasite or preventing a sporozoite invasion or reproduction, preventing or treating coccidiosis, promoting growth performance, controlling, treating and / or preventing necrotic enteritis in a subject, comprising administering to the subject an effective amount of a composition comprising a Crassocephalum rabens (CR), a bioactive extract thereof (CRE), an active ingredient(s) contained in a CR (CR_API) or CRE (CRE_API), a combination of two or more of a CR, a CRE, a CR_API and a CRE_API, or one or more of a-linolenic acid, citric acid, lactic acid, mannitol, palmitic acid, aspartic acid and 1-monomyristin.

The invention belongs to the technical field of biology, and particularly relates to a method for increasing the fucoxanthine accumulation amount by using microcystis subjected to depolymerization treatment to feed palmarthria formosana. According to the method, microcystis subjected to depolymerization treatment are added in the culture process of the palmitothecium for heterotrophic culture, meanwhile, continuous inflation is conducted, the microcystis subjected to depolymerization treatment are swallowed by the palmitothecium, and the higher fucoxanthine accumulation amount in the palmitothecium is obtained by means of the culture mode. The experiment proves that after the microcystis is subjected to pretreatment and depolymerization, the removal efficiency of the microcystis is higher by the brown algae, the limitation of treatment of harmful cyanobacteria and ingestion of protozoa is made up, and meanwhile, an integrated solution is provided for biological treatment of cyanobacterial bloom and resource utilization of the microalgae; meanwhile, a new way is provided for obtaining fucoxanthine with higher accumulation amount.

The application discloses a method for preserving and recovering hydrozoa and medusae, and the method is characterized by the following steps: inducing the hydrozoa to degenerate and enter a dormant state by high salinity, and recovering the hydrozoa from the dormant state in 3 days at the fastest speed when the salinity is restored to 30-35 ppt, and differentiating medusae buds in one week. The method is suitable for preserving and recovering various hydrozoa such as dactylozooid hand medusae, Xiamen anthoathecate medusae and high hand medusae. The method not only solves the problems of long-term preservation and population rejuvenation of hydrozoa, but also effectively removes algae, protozoa, nematodes and other organisms, speeds up the formation and development of medusae buds, greatly saves the manpower and material resources for long-term maintenance of hydrozoa, and improves the efficiency of long-term preservation and maintenance of hydrozoa.

The present invention relates to compositions comprising an inactivated Mycobacterium or an immunogenic fraction thereof, for use in the prevention of an infection in a subject, wherein the infection is selected from the group consisting of a protozoan parasitic infection and a bacterial infection, with the proviso that the infection is not caused by a Mycobacterium.

The application discloses a stable anti-reversibility microbial community structure and a construction method and application thereof, and relates to the technical field of microbial community construction, and in particular to a stable anti-reversibility microbial community structure and a construction method and application thereof.

This invention discloses a method for preparing the multifunctional bioactive peptide Aj-UBL from sea cucumber, relating to the fields of biotechnology and biomedicine. Using the Aj-UBL coding sequence from sea cucumber as a template, the method involves PCR amplification, recombinant expression vector construction, engineered bacterial induction expression, bacterial cell disruption, affinity chromatography purification, and identification to obtain the target peptide possessing triple activities of antibacterial, antiprotozoal, and antitumor activity. This invention employs an optimized coupling process of genetic engineering and affinity chromatography to achieve high-purity, high-quality, and stable product preparation, while being environmentally friendly and free from drug resistance risks. It not only solves the problem of coordinated disease control in sea cucumber farming but also expands the high-value application of sea cucumber resources in the biomedical field, filling the technological gap in the large-scale preparation of multifunctional bioactive peptides from sea cucumber and the biological control of ciliates, possessing significant theoretical and practical application value.

Cryptosporidium parvum is a highly prevalent zoonotic and anthroponotic protozoan parasite that causes a diarrheal syndrome in children and neonatal livestock, culminating in growth retardation and mortalities. Disclosed herein are inhibitors against the enzymatic activity of recombinant CpLDH protein that were identified. The inhibitors were tested for anti-Cryptosporidium effect using in vitro infection assays of HCT-8 cells monolayers. Compounds NSC158011 and NSC10447 were identified to inhibit the proliferation of intracellular C. parvum in vitro, with IC50 values of 14.88 and 72.65 μM, respectively. At doses tolerable in mice, both NSC158011 and NSC10447 significantly reduced the shedding of C. parvum oocysts in infected immunocompromised mice's feces and prevented intestinal villous atrophy as well as mucosal erosion due to C. parvum. These findings have unveiled anti-Cryptosporidium drug candidates that can be explored further for the development of therapeutic agents against C. parvum infections.

The invention discloses protozoa zopoda and application of the protozoa zopoda in promotion of tomato growth and prevention and control of fusarium wilt. The protozoa zopoda cometa NJAU-S1 is preserved in the China Center for Type Culture Collection, and the preservation number of the protozoa zopoda cometa NJAU-S1 is CCTCC (China Center for Type Culture Collection) NO: C2025398. Pot experiment results show that inoculation of protozoa nematopoda NJAU-S1 can significantly promote tomato growth, and plate experiments show that inoculation of protozoa nematopoda NJAU-S1 can inhibit growth of tomato fusarium oxysporum and reduce the infection ability of tomato fusarium oxysporum to tomato, so that the inhibition effect of tomato fusarium oxysporum on tomato growth is relieved. The invention provides a new strategy and resource for biological prevention and control of tomato fusarium oxysporum and scientific planting of tomatoes, and has important application value for safe production of food crops.

Certain embodiments of the invention pertain to a method of treating an infection in a subject caused by an infectious agent other than Escherichia coli, the method comprising administering to the subject arsinothricin or a salt thereof. The infectious agent other than E. coli can be a bacterium, protozoan, helminth, archaebacterium, or a fungus. In preferred embodiments, the infectious agent is Mycobacterium tuberculosis, Mycobacterium bovis, or Enterobacter cloacae. The invention also pertains to a method of treating an infection in a subject caused by an infectious agent, comprising administering to the subject arsinothricin or a salt thereof in combination with an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. In certain such embodiments, the infectious agent expresses phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase. Further embodiments provide compositions comprising arsinothricin or a salt thereof and an inhibitor of phosphinothricin N-acetyltransferase or arsinothricin N-acetyltransferase.

The present application belongs to the technical field of drinking water disinfection, and particularly relates to a method for inactivating ameba and endogenous organisms thereof by electrocatalytic water oxidation. In order to develop a feasible technology for effectively inactivating ameba and endogenous organisms in spores thereof, the present application provides an electrocatalytic water oxidation method for inactivating ameba and endogenous organisms thereof based on advanced oxidation technology. The method can generate strong oxidizing substances such as hydrogen peroxide and hydroxyl radicals in situ, and then inactivate ameba and endogenous bacteria in spores thereof, and the inactivation effect is excellent. Meanwhile, the method has the advantages of simple equipment, convenient operation, low cost, environmental friendliness and the like, and provides a feasible electrocatalytic water oxidation technology for effectively inactivating ameba and endogenous organisms in spores thereof.

This invention discloses a strain of Acanthamoeba that inhibits the pathogen of Fusarium graminearum and its applications. Acanthamoeba (Acanthamoeba rhysodes NJAU-ZY1, deposited at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: C2025219, demonstrates in plate experiments, liquid co-culture experiments, and pot experiments that inoculation with the protozoan Acanthamoeba NJAU-ZY1 significantly inhibits the growth of Fusarium graminearum pathogen, reduces its infectivity in wheat, alleviates the inhibitory effect of Fusarium graminearum on wheat growth, reduces the risk of diseases caused by this pathogen, and thus increases wheat biomass. This provides a new strategy and resource for the biological control of Fusarium graminearum pathogen and the scientific cultivation of wheat, and has important application value for the safe production of food crops.

The present invention discloses a processing method for deep-sea sediments, which relates to the technical field of deep-sea sediment treatment. Through a series of treatments on deep-sea sediments, they can be processed into planting soil and environmentally friendly permeable bricks. Deep-sea soil contains a certain amount of microorganisms including bacteria, fungi, viruses, protozoa, etc. After a series of treatment methods such as pH adjustment, dehydration, solidification, and improvement, the heavy metal content in the deep-sea soil may change and can be used as crop planting soil, which can promote plant growth; the environmentally friendly permeable bricks can, through their special pore structure, quickly infiltrate rainwater into the groundwater layer, reduce waterlogging in the city, and at the same time can also reduce the impact of rainwater runoff on the urban drainage system and reduce the risk of urban waterlogging. The method of the present invention improves the comprehensive utilization efficiency of marine resources.

The invention provides a method for removing protozoa in a chlorella species conservation bottle, which adopts an algae culture tool and seawater to disinfect, and is characterized in that: sodium hypochlorite, bleaching powder or bleaching powder concentrate and other disinfectants containing available chlorine are added to ensure that the concentration of available chlorine in algae reaches 5mg / L. Then, standing the water body for 1 hour so as to settle protozoa to the water bottom; separating the algae liquid on the upper layer into a new container, adding sodium thiosulfate with the addition amount being 1 / 10 of the available chlorine amount in the algae liquid, and neutralizing the residual available chlorine by using the sodium thiosulfate according to the concentration of 0.5 mg / L; after 48 hours of primary treatment, adding sodium hypochlorite, bleaching powder or bleaching powder concentrate and other disinfectants containing available chlorine again for disinfection, continuing to separate the algae liquid, and adding sodium thiosulfate with corresponding concentration again for neutralization; and the protozoa in the breed conservation bottle can be effectively removed after the second treatment.

A determination has been made that apicomplexan tubulin, an essential parasite component, is an excellent drug target. Here, it is shown that it is possible to specifically target parasite tubulins, without harming the mammalian host. A compound has been identified that destabilizes specifically parasite MTs and inhibits essential parasite processes of replication and host cell invasion. This provides a new strategy to tackle the issue of drug resistance development. Specifically, the use of the compound parabulin and / or its derivates in the process of targeting protozoan tubulin for the inhibition of the replication of protozoans in mammalian organism, wherein parabulin has the following molecular weight, sum formula, name and structure:MW: 373.4 Da;Formula: C20H23NO6, ⋅Name (s): 3-(3,4-dimethoxyphenyl)-N-[(3,4,5-trimethoxyphenyl)methyl]prop-2-enamide; (2E)-3-(3,4-Dimethoxyphenyl)-N-(3,4,5-trimethoxyphenyl)acrylamid;SMILES:0(C1═C(OC)C═CC(═C1)C═CC(═0)N(CC2═CC(═C(OC)C(═C2)OC)OC)[H])C.

Probe sets capable of detecting pathogen nucleic acids in a sample are described. The probe set can be provided on a solid support, such as a microarray. Methods of detecting pathogen nucleic acids in a sample using the probe set are also provided. In some examples, the probes and methods are capable of detecting one or more RNA viruses, one or more DNA viruses, one or more bacterial nucleic acids, and / or one or more protozoan nucleic acids in a sample.

The present disclosure is directed to a novel redesigned or retrofitted cabin air filtration system utilizing an ultraviolet light, radiation, or an energy matrix membrane and related technology to eliminate toxins such as viruses, bacteria, mold, fungi, rickettsia, protozoa, etc. from the air system in a vehicle, such as an automobile, marine vehicle airplane, etc., thereby improving the current cabin air filter functionality and providing a sterile air distribution environment throughout the cabin of the vehicle or small area.

The invention relates to the cross technical field of molecular biology and parasitology, and particularly discloses a giardia living body marking method based on fluorescent protein UnaG, which comprises the following steps: firstly, optimizing and synthesizing a UnaG gene sequence according to the codon use preference of giardia; then, constructing a marker plasmid pGT-UnaG-neo on a double expression framework containing an alpha2-tubulin promoter and a gdh promoter, and introducing the plasmid into the giardia giardia trophozoite through electroporation. And carrying out resistance screening in a culture medium containing geneticin to obtain an insect strain capable of stably integrating and expressing UnaG. Through bilirubin induction, efficient maturation and green fluorescence emission of the UnaG protein in giardia cytoplasm are realized. The stably transferred strain obtained by the method has no significant difference from a wild strain in the aspects of growth curve and survival rate, and UnaG and bilirubin have no obvious toxic effect. The limitation that traditional fluorescent protein is inactivated under the anaerobic condition is broken through, and a reliable tool is provided for dynamic imaging, functional research and drug screening of giardia and other anaerobic protozoa.

The present invention discloses a primer-probe combination, method and use for simultaneously detecting three protozoa from giant pandas, belonging to the technical field of protozoal disease detection from giant pandas. The primer-probe combination includes specific primers and TaqMan probes for detecting the three protozoa, and the specific sequences are shown in SEQ ID NOs. 1 to 9. The present invention designs three pairs of specific primers and three TaqMan probes for the specific regions of Babesia giant panda, Hepatozoon spp., and Toxoplasma gondii, and establishes a triple fluorescent quantitative PCR detection method that can simultaneously identify Babesia giant panda, Hepatozoon spp., and Toxoplasma gondii in a single PCR. The detection method has good specificity and accuracy, high sensitivity, high detection efficiency, no cross-reaction, and saves time and sample usage, providing a new option for the simultaneous detection of three protozoa from giant pandas.

The invention discloses a complex microbial inoculant, a biological stimulant, a preparation method and application. The composite bacterial agent disclosed by the invention comprises Pristia aryabhattai P28 (the preservation number is CGMCC (China General Microbiological Culture Collection Center) 1.65035), bacillus subtilis S1 (the preservation number is CGMCC 1.65034) and bacillus velezensis C1 (the preservation number is CGMCC 1.65033), and the volume ratio of the P28 to the S1 to the C1 is 3 to 1 to 2. The biological stimulant is prepared from kitchen waste aerobic hydrolysate which is obtained by fermenting organic waste kitchen waste serving as a raw material with a complex microbial inoculant with the addition amount of 5% by volume. After the biological stimulant is applied to soil, the total amount of soil organisms (bacteria, fungi and protozoa) can be effectively increased, the soil aggregate stability is improved, and the soil structure is rapidly improved; the content of total organic carbon, granular organic carbon and soluble organic carbon in soil and the activity of soil enzymes (catalase, sucrase, urease and acid phosphatase) are obviously improved in a short time. The method disclosed by the invention has important significance on resource utilization of organic wastes and agricultural sustainable development.

Disclosed are advantageous systems and methods for treating building water systems, especially the interior surfaces of premise plumbing, to remove biofilm and inactivate biofilm-associated pathogens, including protozoa, using disinfectant formulations at concentrations at in excess of those used for drinking water treatment, and further, in co-applying complexing agents to mitigate corrosion of the materials treated; and using these in conjunction with off-gas containment devices that allow flushing of taps without the liberation of toxic fumes.