110 results about "Necrotic enteritis" patented technology

A feed additive composition comprising a direct fed microbial in combination with a protease, and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease and a phytase.

A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving dietary phosphorus absorption and retention, or for improving the efficacy of the phytase, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject at least one direct fed microbial in combination with a phytase, wherein the phytase is administered to the subject at a dosage of more than about 1500 FTU / kg feed.

A feed additive composition comprising a direct fed microbial in combination with a protease, a xylanase, an amylase and a phytase, and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for avoiding the negative effects of necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject, which method comprising administering to a subject a direct fed microbial in combination with a protease, a xylanase, an amylase and a phytase.

There is provided a vaccine for controlling Clostridium perfringens in animals, and particularly necrotic enteritis in poultry. The vaccine may comprise a C. perfringens antigenic polypeptide or variant thereof, a nucleic acid encoding the C. perfringens antigenic polypeptide or variant thereof, or a recombinant cell producing the C. perfringens antigenic polypeptide or variant thereof.

The present invention provides methods of inducing an immune response against Clostridium species in birds, for protecting birds from Clostridium infection, and / or for protecting birds from related disorders such as necrotic enteritis. The methods can be practiced in ovo and / or post-hatch. The invention further provides compositions and methods for delivery of a composition of this invention in ovo directly to the embryo body.

A feed additive composition comprising a direct fed microbial (DFM) in combination with a phytase derivable from Citrobacter spp. and a method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for avoiding the negative effects of necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject, or for promoting the growth of beneficial bacteria in the gastrointestinal tract of a subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject a direct fed microbial (DFM) in combination with a phytase derivable from Citrobacter spp.

The present invention provides methods of inducing an immune response against Clostridium species in birds, for protecting birds from Clostridium infection, and / or for protecting birds from related disorders such as necrotic enteritis. The methods can be practiced in ovo and / or post-hatch. The invention further provides compositions and methods for delivery of a composition of this invention in ovo directly to the embryo body.

The invention discloses Bacillus licheniformis H2 which is resistant to acid, heat and cholate and has good resistance to Clostridium perfringens and application of Bacillus licheniformis H2 in prevention and control of necrotic enteritis in chicken. The strain is obtained by separation, screening and identification, and has the preservation number of CCTCC NO: M2011133; and Bacillus licheniformis H2 can be prepared into a microorganism preparation, and can effectively prevent and control necrotic enteritis in chicken caused by Clostridium perfringens, promote growth of normal physiological anaerobic bacteria in intestinal tract of chicken, improve intestinal tract microorganism diversity and immune organ index of chicken and improve growth performance of chicken when used as a veterinary medicine and chicken feed additive. Bacillus licheniformis H2 is suitable for feed factories, veterinary drug factories and scaled chicken farms.

The invention discloses a traditional Chinese medicine for treating bacterial diseases in poultry, which is prepared from the following raw materials in parts by weight: 10-15 parts of wild chrysanthemum flower, 5-10 parts of Chinese goldthread, 8-10 parts of Scutellaria baicalensis, 8-10 parts of golden cypress, 10-15 parts of Chinese thorowax, 10-12 parts of Cape jasmine, 20-25 parts of Fagopyrum cymosum root, 25-30 parts of common andrographis herb, 8-10 parts of burdock, 25-30 parts of Chinese pulsatilla root and 30-35 parts of folium artemisiae argyi. The traditional Chinese medicine for treating the bacterial diseases in the poultry, disclosed by the invention, has the effects of preventing and treating the bacterial diseases caused by colibacillosis, salmonellosis, necrotizing enterocolitis, enterovirus syndromes, cholera fowls and the like in the poultry.

The invention discloses bacillus subtilis with an effect of inhibiting clostridium perfringens; the bacterial strain is named the bacillus subtilis BLCC1-0155. The bacillus subtilis BLCC1-0155 disclosed by the invention has a powerful inhibiting effect on the clostridium perfringens, and also has the inhibiting effect on escherichia coli and staphylococcus aureus. Proved by an animal experiment, a microecologics product taking the bacillus subtilis BLCC1-0155 as an effective component is capable of preventing necrotic enteritis of broilers better, therefore the hurt of the necrotic enteritis to intestinal tracts of the broilers is reduced, and the death rate is greatly reduced; meanwhile, the microecologics product is also capable of increasing the daily gain of the broilers, and is a relatively ideal microecologics product, which can be clinically applied, used for preventing the necrotic enteritis of the broilers.

Disclosed herein are compositions for the treatment of a disease in an animal including yeast extract of Saccharomyces cerevisiae, Bacillus licheniformis or Bacillus subtilis spores, and a carrier. Also included are animal feed compositions including the composition for the prevention, control and / or treatment of a disease in an animal and an animal's food / feed. The compositions are useful to prevent, control, and treat diseases such as necrotic enteritis in poultry when used in combination with an anticoccidal ionophore or coccidiosis vaccine.

The invention discloses a traditional Chinese medicine feed additive for treating chicken coccidiosis. The selected traditional Chinese medicine raw materials include 6 parts of cimicifuga foetida, 5 parts of scutellaria baicalensis, 6 parts of sophora flavescens, 6 parts of the root of Chinese pulsatilla, 3 parts of java brucea, 3 parts of antifebrile dichroa, 6 parts of artemisia apiacea and 5 parts of radix stemonae by weight. A preparation method is characterized by mixing the raw materials and then grinding or grinding the materials and then mixing. The selected raw materials play roles of clearing away heat and toxic materials and getting rid of insects and stopping itching together. The aims of preventing and treating chicken coccidiosis, necrotic enteritis and leucocyto zoonosis are achieved by daily feeding the feed. Through application, the feed additive is found to have the advantages of good prevention and treatment effects, no toxic or side effects, easily obtained raw materials and low price.

The invention discloses a composition comprising at least one long chain polyunsaturated fatty acid, at least one probiotic and a mixture of oligosaccharides, said mixture containing at least one N-acetylated oligosaccharide, at least one sialylated oligosaccharide and at least one neutral oligosaccharide, for use in the promotion of intestinal angiogenesis and of nutrient absorption and of enteral feeding tolerance and / or in the prevention and / or treatment of intestinal inflammation, such as necrotizing enterocolis, and / or in the recovery after intestinal injury and / or surgery. This composition is particularly adapted for use in infants, notably preterm infants.

The invention discloses a traditional Chinese medicinal feed additive for treating chicken coccidiosis, which is prepared from the following traditional Chinese medicinal materials: baical skullcap root, lightyellow sophora root, sessile stemona root, hawthorn, indigowoad root, skunk bugbane and golden thread. A preparation method for the traditional Chinese medicinal additive comprises the following steps of: mixing the traditional Chinese medicinal materials, and crushing; or crushing the traditional Chinese medicinal materials respectively, and mixing. The selected traditional Chinese medicinal materials are combined to achieve the effects of clearing heat and releasing toxin, and killing coccidium and relieving itching. Through daily feeding, the traditional Chinese medicinal feed additive fulfills the aim of preventing and controlling the chicken coccidiosis, enteritis necroticans and chicken white crown. Through application, the traditional Chinese medicinal feed additive has the advantages of good prevention and control effect, no toxic or side effect, readily available materials and low price.

The invention discloses lactobacillus plantarum Lac16, the preservation name is Lactobacillus plantarum Lac16, and the preservation number is CCTCC NO: M 2016259. The lactobacillus plantarum can prevent occurrence of necrotic enteritis and inhibit growth of clostridium perfringens, so that the problem that production performance of meat chickens is lowered due to attacking toxin of the clostridium perfringens is relieved, and the structural damage of the intestinal mucosa is relieved; immunity of intestinal mucosa of chickens can also be improved, and growth of the meat chickens can be promoted.

The invention belongs to the technical field of broiler chicken breeding and particularly relates to a method for breeding 3-to-7-week-old broiler chickens. The method specifically includes the following steps: 1, daily exercise outside a chicken house, wherein during 8:00-11:00 every day, the chickens are allowed to jump to a horizontal mesh from a horizontal platform once every15 min, and during 14:00-17:00, the chickens are allowed to jump to the horizontal mesh from the horizontal platform once every 20 min; 2, daily feeding in the chicken house, wherein the chickens are regularly fed with feed at 7:00, 11:40,18:10 and 23:40 every day, and the chickens are fed with a nutrient solution prepared from lycium barbarum polysaccharide and strawberry powder 15 min before feeding the chickens with the feed at a time. According to the method, the Escherichia coli disease rate of the broiler chickens is reduced to 1-1.5%, the necrotic enteritis disease rate is reduced to 1% to 1.5%, the daily gain reaches 98.31-98.88 g, the slaughter rate reaches 87.34%-87.43%, and the feed conversion ratio is decreased to 1.55-1.58.

Gut health in poultry is improved by providing an efficacious amount of extracts of tannic acid via feed or via drinking water. The extracts of tannic acid are also effective when the poultry are infected with coccidiosis and / or necrotic enteritis. In alternative embodiments, an efficacious amount of Bacillus coagulans is also included.

Applicants have examined two Clostridium species, Clostridium difficile and Clostridium perfringens, and found that clays can adsorb the toxin produced by both and that a clay blended product can decrease the effects of a prominent in chickens and known as Necrotic Enteritis, that is caused by C. perfringens. Recently a clay or a clay blend that may be a combination of clay, yeast, and a form of a functional amino acid, was examined and found to help decrease the effects of acute hepatopancreatic necrosis disease (AHPND), which is also known as Early Mortality Syndrome (EMS) in shrimp when a challenge model that included Vibrio parahaemolyticus was used.

The invention relates to a preparation method for a necrotic enteritis (Type A) inactivated vaccine for producing a synthetic culture medium. In China, an inactivated vaccine specific to necrotic enteritis is unavailable; and a sarco-liver-stomach enzyme digesting soup culture medium is generally adopted specific to clostridium disease inactivated vaccines of other animals, mainly comprises beef, liver and the like, and is influenced greatly by the varieties, ages, freshness and digesting degrees of animals; and under the influences of the varieties, ages, freshness and digesting degrees of animals, instable quality, high dispersing degree among batches and complexity in a manufacturing process are caused, the quality of a vaccine is influenced, and particularly the homogeneity of the vaccine is influenced. According to a synthesized culture medium provided by the invention, the defects are overcome, and production of a high-quality necrotic enteritis (Type A) inactivated vaccine is ensured powerfully; and the inactivated vaccine is the developing trend of bacteria high-cell-density culture, and has the advantages of easiness and convenience for operating and stable quality.

The invention relates to the field of pharmaceutical chemistry, specifically to thymol ester compounds (I), its preparation method and an application thereof. It is proved through a pharmacology experiment that the thymol ester compounds (I) provided by the invention can be used to prepare medicines for treating or preventing diseases caused by bacteria or diseases caused by fungi and can be also used to prepare animal feed additives or drinking water tonifying formula for treating, mitigating or preventing necrotic enteritis caused by Shigella species, clostridium and Clostridium perfringens. The invention also discloses a preparation method of the derivatives and their pharmaceutical composition.

The invention discloses application of licochalcone A to preparation of a medicine for treating necrotic enteritis. A clostridium perfringens sliding motion inhibition test, a biofilm formation inhibition test and a chick necrotic enteritis model test verify that the licochalcone A has protective effect on chicken necrotic enteritis caused by infection of clostridium perfringens. Due to abuse of the traditional antibiotics and bacterial medicine resistance, the licochalcone A has the characteristics of high curative rate, no medicine resistance and no medicine residues, and the licochalcone Acan improve the selectivity of the used medicines and has important significance in developing new medicines when being used for treating the chicken necrotic enteritis.

The invention discloses a traditional Chinese medicine composition for treating necrotic enteritis of chickens. The traditional Chinese medicine composition is mainly prepared from golden cypress, fructus aurantii, magnolia obavata, radix linderae, pericarpium arecae, rhizoma atractylodis macrocephalae, poria cocos, rhizoma dioscoreae, fructus crataegi, radix sophorae flavescentis, medicated leaven, radix sileris, herba patriniae, radix pulsatillae, moutan bark, selfheal, radix glycyrrhizae, nauclea officinalis, folium perillae, cassia occidentalis, radix astragali, pericarpium granati, monkshood, herba taraxaci, bunge corydalis herb, red triadica sebifera and radix scutellariae. A preparation method of the traditional Chinese medicine composition for treating necrotic enteritis of chickens comprises the following steps: superfine grinding, mixing in batches, extracting, combining and concentrating. The traditional Chinese medicine composition has the advantages that the curative effect is effective, the medicine resistance is avoided, the medicine residue is avoided, and the traditional Chinese medicine composition is capable of treating necrotic enteritis of chickens, improving intestinal environment of chickens and enhancing immunity of chickens.

The invention discloses a traditional Chinese medicine feed additive for preventing necrotic enteritis and a preparation method thereof. The traditional Chinese medicine feed additive for preventing necrotic enteritis is prepared from the following common raw materials in parts by weight: 17-21 parts of rhizoma polygonati, 14-28 parts of polygonum multiflorum thumb, 10-14 parts of cactus, 10-14 parts of pine needle, 7-11 parts of radix sophorae flavescentis, 6-10 parts of Chinese pulsatilla root, 6-10 parts of air potato, 5-11 parts of honeysuckle and 5-11 parts of schisandra chinensis. The preparation method comprises the following steps: proportionally mixing the medicinal materials, and processing into powder with the granularity of 50-70 meshes; adding 30% purified water to the powder to naturally ferment into enzyme together with a proper amount of brown sugar; grinding the rest of dreg into a paste by virtue of a high-speed pulping machine after an enzyme solution is extracted; proportionally mixing the paste with other feed raw materials; fully mixing with purified excellent bacillus and excellent saccharomycetes; and fermenting again for 36 hours, so as to prepare the traditional Chinese medicine feed additive for preventing the necrotic enteritis. The traditional Chinese medicine feed additive has the effects that the traditional Chinese medicine feed additive is mild in drug properties and easy to absorb, the morbidity and mortality of the necrotic enteritis are obviously reduced, and the feed conversion ratio is lowered.

The invention relates to a clostridium perfringen alpha toxin genetic engineering vaccine and application thereof. The clostridium perfringen alpha toxin genetic engineering vaccine is prepared by designing a specific primer, implementing polymerase chain reaction (PCR) amplification on A-type clostridium perfringen DNA to obtain an alpha toxin gene segment, cloning the gene segment onto a pMD18-T Vector, selecting a positive recon and linking the positive recon onto a PET-28 alpha (+) plasmid; introducing the recombinant plasmid to a receptor strain to successfully construct a recombinant strain BL21 (DE3)-alpha; emulsifying the prepared alpha toxin protein and an aluminum hydroxide adjuvant at a volume ratio of 9:1, and adding 0.01% thiomersal to obtain the clostridium perfringen alpha toxin genetic engineering vaccine. The clostridium perfringen alpha toxin genetic engineering vaccine is easy for realization of industrial production, simple to operate and good in safety, and is capable of effectively preventing such diseases as animal necrotic enteritis, enterotoxemia, and human and animal traumatic gas gangrene caused by the A-type clostridium perfringens.

The invention discloses a composition for preventing necrotizing enterocolitis and a use thereof, and belongs to the field of biological medicine. The composition of the invention includes selecting from one or more separated lactobacillus strains, wherein the lactobacillus strains are selected from a group composed of lactobacillus plantarum subsp.plantarum PM-A0087 strains (preserved in NRRL, with a preservation number of NRRL-B50523), bifidobacterium longum PM-A0101 strains (preserved in NRRL, with a preservation number of NRRL-B50525) and bifidobacterium bifidum PM-A0218 strains (preserved in NRRL, with a preservation number of NRRL-B50524). The invention also discloses the use of the composition containing any one of the lactobacillus strains or combinations of the lactobacillus strains in preparation of drugs for preventing necrotizing enterocolitis.

The invention discloses a compound micro-ecological preparation for preventing and treating necrotic enteritis of poultry and application of the compound micro-ecological preparation. The compound micro-ecological preparation is compounded by taking bacillus velezensis, lactobacillus plantarum and xylooligosaccharide as active ingredients. The bacillus velezensis and lactobacillus plantarum strains have a relatively strong inhibition effect on intestinal pathogens such as clostridium perfringens, salmonella and escherichia coli, and are combined with the intestinal probiotic function of xylooligosaccharide, so that the intestinal health of poultry can be adjusted, necrotic enteritis of poultry can be effectively prevented and treated, and the use of antibiotics in the breeding process is reduced.