Medicine for treating or preventing chicken coccidiosis and chicken necrotic enteritis and application

Abstract

The invention belongs to the technical field of medicine, and particularly relates to a medicine for treating or preventing chicken coccidiosis and chicken necrotic enteritis and application, the medicine is chaulmoogratree seed, and can be in the form of medicinal material powder or chaulmoogratree seed extract. The traditional Chinese medicine composition can be used for preventing and treating chicken coccidiosis diseases, reducing the death rate of broilers with necrotic enteritis and intestinal lesions of necrotic enteritis, effectively improving chicken necrotic enteritis and has a good popularization and application prospect in the aspect of preventing and treating chicken necrotic enteritis diseases; the traditional Chinese medicine composition has a good treatment effect on mixed infection diseases of small intestine coccidiosis and clostridium.

Description

Technical Field

[0001] The present application belongs to the field of medical technology, and specifically relates to drugs and uses for treating or preventing chicken coccidiosis and chicken necrotic enteritis. Technical Background

[0002] Coccidia is a type of protozoa. The academic community generally divides coccidia into several species, namely Eimeria tenella, Eimeria acervulina, Eimeria giant, Eimeria toxicophila, Eimeria brunneri, Eimeria mite and Eimeria precocious. Chicken coccidiosis is a global parasitic disease with extremely serious harm, which causes huge economic losses to the poultry industry every year. Intensive chicken farms are the most suitable places for coccidiosis outbreaks, with high morbidity and mortality rates. Existing anticoccidial drugs are increasingly developing resistance, and the prevention and control of chicken coccidiosis requires the development of new anticoccidial drugs.

[0003] Necrotic enteritis in chickens is a type of enteritis caused by Clostridium perfringens, also known as clostridial enteritis dysbacteriosis or intestinal bacterial overgrowth. The dead birds are characterized by intestinal flatulence and intestinal bleeding. With the ban on antibiotic growth promoters, the disease has occurred in major poultry-raising countries and regions around the world, causing great economic losses. In 1930, Bennetts isolated Clostridium perfringens (also known as Clostridium perfringens or Clostridium perfringens) from the intestines of black Orpington pullets, and chickens infected with this bacterium can be fatal. Oral inoculation of Clostridium perfringens can replicate intestinal disorders in young roosters. Necrotic enteritis is mainly caused by type A or type C Clostridium perfringens, causing characteristic lesions such as intestinal mucosal necrosis and bleeding, but type C is not common. Both type A and type C Clostridium perfringens can produce alpha toxins, and type C can also produce beta toxins. Clostridium perfringens is an anaerobic bacterium that can produce spores and is Gram-positive. It can move through pili. It is a commensal bacterium in the intestinal tract of vertebrates and can be isolated from fecal-contaminated environments.

[0004] Fructus dasyphyllae is the dried mature seeds of Fructus dasyphyllae of the family Fructus dasyphyllae. The structural types of compounds isolated from Fructus dasyphyllae are mainly phenylpropanoids, flavonoid lignans and fatty acids. Fructus dasyphyllae is pungent and hot in nature and enters the lung, spleen and liver meridians. It has the function of dispelling wind and drying dampness. It is mainly used to treat scabies, itching of bedsores, and swelling and poison of sores. However, there are no reports on its efficacy in preventing and treating coccidiosis and necrotic enteritis in chickens, and there are no reports on cases of mixed infection of the two diseases. Summary of the invention

[0005] The present invention discloses a medicine for treating or preventing chicken coccidia and chicken necrotic enteritis and its use, provides a new use of Psoralea corylifolia, and the specific technical scheme is as follows:

[0006] Use of Fructus cyrtonemae in preparing medicine for preventing or treating chicken coccidiosis.

[0007] The chicken coccidiosis is a chicken Eimeria coccidiosis, preferably Eimeria tenella, Eimeria acervulina, Eimeria maxima, Eimeria toxicans, Eimeria brucei, Eimeria mitis and / or Eimeria precocious. More preferably, it is a chicken Eimeria toxicans coccidiosis.

[0008] The invention discloses a use of Fructus dasyphyllae in preparing a medicine for preventing or treating necrotic enteritis in chickens, wherein the necrotic enteritis in chickens is preferably caused by Clostridium perfringens.

[0009] Use of Fructus sylvestris in preparing medicine for preventing or treating chicken coccidiosis and chicken necrotic enteritis mixed disease.

[0010] The Fructus Cynoglossi described in the present invention is a medicinal powder or a Fructus Cynoglossi extract, and the Fructus Cynoglossi extract is preferably a water extract or an alcohol extract.

[0011] In the present invention, the medicament is prepared by combining the Herba Cynanchifoliae with other medicaments.

[0012] Compared with the prior art, the present invention has the following beneficial technical effects:

[0013] The invention can prevent and treat chicken coccidiosis, reduce the mortality of broiler chickens with necrotic enteritis and the intestinal lesions of necrotic enteritis, effectively improve chicken necrotic enteritis, and has good promotion and application prospects in preventing and treating chicken necrotic enteritis; it has a therapeutic effect on small intestinal coccidiosis and clostridial mixed infection diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 : Watery stools, red stools;

[0015] Figure 2 : The intestines turn black, the intestinal wall becomes thinner, the intestines are bloated, and there are red stools in the intestines;

[0016] Figure 3 : The first observation point of Building 6;

[0017] Figure 4 : The second observation point of Building 6;

[0018] Figure 5 : The third observation point of Building 6;

[0019] Figure 6 : The first observation point of Building 5;

[0020] Figure 7 : The second observation point in Building 5;

[0021] Figure 8 : The third observation point in Building 5;

[0022] Fig. 9 : Red stool;

[0023] Fig.10 : Yellow ulcers in the liver, thinning and bloating of some intestinal walls;

[0024] Fig.11 : The first observation point;

[0025] Fig.12 : The second observation point. DETAILED DESCRIPTION

[0026] The present invention can be better understood according to the following examples. However, it is easy for those skilled in the art to understand that the contents described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0027] 1. Evaluation test report of different drugs on the toxicity of Eimeria to chickens

[0028] 1. Purpose of the experiment

[0029] The effects of each example at the recommended dose on infection with Eimeria nematophila GD strain were evaluated.

[0030] 2 Test materials

[0031] 2.1 Experimental insect strains

[0032] The GD strain of Eimeria toxicum was isolated and preserved in the Parasitic Biology Laboratory, Institute of Animal Health, Guangdong Academy of Agricultural Sciences.

[0033] 2.2 Trial Drugs

[0034] Each embodiment is provided by Beijing Shengtaier Technology Co., Ltd.

[0035] Example 1: Take 1000g of Fructus Caryophylli, add 10 times the amount of water, decoct twice, each time for 1 hour, combine the decoctions, concentrate to 1000mL, centrifuge to remove impurities, and make up to 1000mL.

[0036] Example 2: Take 1000g of Coptis chinensis, add 10 times the amount of water, decoct twice, each time for 1 hour, combine the decoctions, concentrate to 1000mL, centrifuge to remove impurities, and fix the volume to 1000mL.

[0037] Example 3: Take 1000g of Phellodendron chinense, add 10 times the amount of water, decoct twice, each time for 1 hour, combine the decoctions, concentrate to 1000mL, centrifuge to remove impurities, and fix the volume to 1000mL.

[0038] Example 4: Take 1000g of betel nut, add 10 times the amount of water, boil twice, each time for 1 hour, combine the decoctions, concentrate to 1000mL, centrifuge to remove impurities, and fix the volume to 1000mL.

[0039] Example 5: Take 1000g of Radix Dichroae, add 10 times the amount of water, decoct twice, each time for 1 hour, combine the decoctions, concentrate to 1000mL, centrifuge to remove impurities, and fix the volume to 1000mL.

[0040] Example 6: Take 1000g of Radix Codonopsis pilosulae, grind it and pass it through a 40-mesh sieve.

[0041] Example 7: Take 1000g of Coptis chinensis medicinal material, crush it, and pass it through a 40-mesh sieve.

[0042] Example 8: Take 1000 g of Phellodendron chinense medicinal material, crush it, and pass it through a 40-mesh sieve.

[0043] Example 9: Take 1000 g of betel nut medicinal material, crush it, and pass it through a 40-mesh sieve.

[0044] Example 10: Take 1000 g of Radix Dichroae, grind it and pass it through a 40-mesh sieve.

[0045] 2.3 Experimental animals and feed

[0046] One-day-old 818 fast-growing broiler chickens were purchased from the Sanhuang Farm of Wens Group; 161Q complete broiler feed, which did not contain any anticoccidial drugs and antibacterial drugs, was purchased from the Experimental Feed Factory of Wens Group.

[0047] 3 Test methods

[0048] 3.1 Experimental design and grouping

[0049] The experimental chicks were raised to 12 days old, and after weighing each chick, the thin or overweight chicks were eliminated, and healthy chickens with individual weight differences within 20g were selected and randomly divided into 7 groups, with 21 in each group. The groups of Examples 6-10 were drug-treated and insect-fighting groups, and a model group without drug-fighting and insect-fighting and a blank control group without drug-fighting and insect-fighting were also set up. Each drug test group began to be medicated at 12 days of age, and the groups of Examples 6-10 were medicated by mixing feed with feed. Each chicken in each drug test group and the non-drug insect-fighting test group was orally inoculated with 1.0×10 4 All the experimental chickens in the groups were given free access to food and water until the end of the experiment on the 7th day, as shown in Table 1.

[0050] Table 1 Experimental design grouping

[0051]

[0052] 3.2 Evaluation criteria for anticoccidial index

[0053] 3.2.1 Scoring of bloody stools

[0054] Feces were scored according to the method of Suo Xun (1997). On the 6th day after the attack, the proportion of bloody stools was observed, photographed and recorded, and bloody stools were observed and scored for Eimeria toxicus: 0 points means 100% of the feces were free of blood, +1 point means 25% of the feces were bloody, +2 points means 50% of the feces were bloody, +3 means 75% of the feces were bloody, and +4 points means 100% of the feces were bloody.

[0055] 3.2.2 Relative weight gain rate

[0056] The chickens were weighed at the beginning and end of the experiment, and the average weight gain and relative weight gain rate were calculated. Relative weight gain rate = (weight gain rate of the experimental group / weight gain rate of the experimental group without medication or insect attack) × 100%.

[0057] 3.2.3 Survival rate

[0058] The number of dead chickens in each group was recorded, the cause of death was determined by autopsy, and the survival rate was calculated. Survival rate = (number of surviving chickens at the end of the experiment / number of chickens in the experimental group) × 100%.

[0059] 3.2.4 Lesion value

[0060] The chickens were slaughtered on the 7th day after infection, and the jejunal intestinal lesions of each chicken were scored according to the lesion scoring method designed by Johnson and Reid (1970), and the lesion scores were converted into lesion values. The lesion scoring standard is:

[0061] 0 points: no visible lesions;

[0062] +1 point: Pinpoint-sized scattered bleeding spots and white spots can be seen on the serosal surface of the middle part of the small intestine, a small amount of orange-red contents can be seen in the intestinal cavity, and the intestinal wall is slightly thickened;

[0063] +2 points: Intestinal wall thickening, a large number of hemorrhagic spots and white spots on the serosal surface, mild bloating in the middle of the small intestine, and blood clots mixed in the intestinal cavity;

[0064] +3 points: The serosal surface is covered with red hemorrhages and white spots, the intestinal cavity is inflated and dilated, the intestinal wall is obviously thickened, the entire intestinal contents contain a large amount of blood clots and necrotic and detached epithelial tissue, the intestinal mucosal surface is rough, and there is no normal intestinal content;

[0065] +4 points: The middle part of the small intestine is highly swollen, the intestinal segment is atrophic and significantly shortened, the lesions extend to the duodenum and the posterior part of the small intestine, the small intestine contains soy sauce-colored or brown mucus, the intestinal mucosa is bleeding and necrotic. Chickens killed by this coccidia are scored as +4 points.

[0066] Lesion value (0-40) = average lesion score of each test group (0-4) × 10.

[0067] 3.2.5 Oocyst value and relative oocyst yield (ROP)

[0068] The fecal oocyst count was performed using the McMaster counting method to obtain the number of oocysts per gram of feces (OPG) for each group, and the oocyst value was calculated according to Table 2.

[0069] Relative oocyst production (ROP) = (average oocyst production of the infected drug-administered group / average oocyst production of the infected non-drug-administered group) × 100%. ROP ≥ 15% indicates drug resistance; ROP < 15% indicates no drug resistance.

[0070] Table 2 Conversion relationship between oocyst number and oocyst value

[0071]

[0072] 3.2.6 Anticoccidial index (ACI)

[0073] Referring to the calculation formula of Merck & Co., Inc. in the United States, that is:

[0074] ACI = (relative weight gain rate + survival rate) × 100 - (lesion value + oocyst value) is calculated.

[0075] Pharmacodynamic judgment criteria: ACI > 180 belongs to highly effective anticoccidial drugs; 160 < ACI < 180 belongs to moderately effective anticoccidial drugs; 120 < ACI < 160 belongs to low-effective anticoccidial drugs; ACI < 120 is ineffective against coccidia.

[0076] 4 Test results

[0077] 4.1 Clinical symptoms

[0078] After the experimental chickens in the challenge control group were infected with sporulated oocysts, they gradually showed reduced food intake, poor spirit and other reactions. On the 5th day after infection, except for the group of Example 6, each drug group and the model group were affected to varying degrees, with reduced feed consumption and water intake; on the 7th day, the jejunum lesions were evaluated by dissection, with varying degrees of bleeding or orange-red contents, and no lesions were observed in other organs; there was no bloody feces in the blank control group, and the food intake and water intake were normal.

[0079] 4.2 Results of bloody feces scoring and jejunum lesions

[0080] From the fecal observation results on the 6th day after challenge, the bloody feces in the model group showed +2 points; the bloody feces in Examples 7 - 10 showed +1 point; there was no bloody feces in Example 1 and the blank control group, showing 0 point.

[0081] 4.3 Results of drug sensitivity test

[0082] The results suggest that Example 1 had the lowest relative oocyst production (ROP) of Eimeria necatrix GD strain oocysts, showing a certain effect against Eimeria necatrix, followed by a certain improvement in the ROP performance of Example 5; the ROP and lesion values of other examples were not ideal and were judged as ineffective. The detailed results are shown in Table 3.

[0083] Table 3 Drug sensitivity test results

[0084] Group Relative weight gain rate / % Survival rate / % Lesion value Oocyst value ACI value ROP Example 6 93.00 100.00 5.00 5.00 183 10.37 Example 7 80.76 100.00 10.00 10.00 135.76 66.97 Example 8 105.09 100.00 10.00 10.00 155.09 60.25 Example 9 99.73 100.00 21.00 40.00 138.73 100.40 Example 10 101.49 100.00 22.00 20.00 160.49 41.85 Model Group 88.97 100.00 22.00 40.00 126.97 100.00 Blank control group 100.00 100.00 0.00 0.00 200.00 0.00

[0085] 5 Conclusion

[0086] 5.1 The drug in this study was subjected to anticoccidial animal susceptibility test at the recommended dosage concentration. The results showed that among Examples 6-10, Example 6 had the lowest oocyst production (ROP) against the GD strain of Eimeria toxicans, and had an inhibitory effect; compared with Examples 7-10, it had a more prominent anticoccidial effect.

[0087] 2. The effect of different drugs on the prevention and treatment of enteritis caused by Clostridium perfringens infection in chickens

[0088] 1. Purpose of the experiment

[0089] The effects of each embodiment on relieving symptoms such as enteritis caused by Clostridium perfringens infection in chickens and the effects on the production performance of chickens were evaluated at the recommended drinking water dosage.

[0090] 2 Test materials

[0091] 2.1 Test strains

[0092] Chicken Clostridium perfringens was purchased from the China Veterinary Drug Administration.

[0093] 2.2 Trial Drugs

[0094] Test drugs: Examples 1-5, homemade, medicinal materials purchased from Anguo Jufu Chinese Medicinal Materials Co., Ltd., pharmacopoeia standards.

[0095] Control drug: 5% lincomycin solution. Lincomycin was purchased from Shandong Yakang Pharmaceutical Co., Ltd. and prepared into a liquid for oral administration.

[0096] 2.3 Experimental animals and feed

[0097] The chickens used in the experiment were 1-day-old 818 broiler hens. The 161Q corn broiler complete feed, which did not contain any anticoccidial drugs and antibacterial drugs, was specially prepared by the feed factory.

[0098] 3 Test methods

[0099] 3.1 Experimental design and grouping

[0100] The experimental chicks were grouped from 1 day old, weighed one by one, thin or overweight chicks were eliminated, healthy chickens with individual weight differences within 30g were selected, and randomly divided into 8 groups, each with 21. Groups 1-5 were drug groups of Examples 1-5, group 6 was control drug lincomycin, group 7 was a model group with clostridial infection and no medication, and group 8 was a blank control group without infection and no medication.

[0101] Each drug-administered test group (Groups 1-6) was given the recommended dose of drinking water from 8 days of age to the end of the experiment; Groups 1-7 were given 7.5×10 8 CFU / mL of Clostridium perfringens.

[0102] The experimental chickens were fed with corn broiler complete feed throughout the experiment. They were allowed to drink water and eat feed freely until the end of the experiment on the 20th day of age. See Table 4 for details.

[0103] Table 4 Experimental design grouping

[0104]

[0105]

[0106] 3.2 Evaluation criteria for enteritis and production performance indicators

[0107] 3.2.1 Scoring criteria for intestinal lesions

[0108] At 20 days of age, the intestines (duodenum, upper jejunum) of chickens in each group were taken for lesion scoring (the highest score was the total intestinal lesion score). The scoring criteria were as follows: 0 = no obvious lesions; 1 = intestinal mucosal congestion; 2 = small focal necrosis or ulceration (1-5 lesions); 3 = focal necrosis or ulceration (6-15 lesions); 4 = focal necrosis or ulceration (16 or more lesions).

[0109] Lesion value (0-40) = average lesion score of each test group (0-4) × 10.

[0110] 3.2.2 Relative weight gain rate

[0111] Weigh the chickens at 20 days of age and calculate the average weight gain and relative weight gain rate. Relative weight gain rate = (average weight gain of the experimental group / average weight gain of the blank group) × 100%.

[0112] 3.2.3 Survival rate

[0113] The number of dead chickens in each group was recorded, the cause of death was determined by autopsy, and the survival rate was calculated. Survival rate = (number of surviving chickens at the end of the experiment / number of chickens in the experimental group) × 100%.

[0114] 4 Test results

[0115] 4.1 Clinical symptoms

[0116] After the infection, the chickens had decreased feed intake, poor mental state, and weight loss. On the third day after infection, the model group and Example 2-5 groups all had varying degrees of loose feces discharge, and the amount of water they drank decreased to varying degrees; the blank group had normal feed intake and water drinking.

[0117] 4.2 Intestinal lesions

[0118] After 5 days of challenge with bacteria from the 14th to the 18th day, the intestinal lesions of the duodenum and jejunum of the experimental chickens in each group were observed. The results showed that the duodenum and jejunum of the model group and Example 2-5 groups all had varying degrees of damage.

[0119] 4.2 Intestinal lesion scoring results

[0120] The experimental results showed that the lesion results of the duodenum: the lesion value of the blank control group was the lowest, and the lesion value of the model group was the highest. In addition, the lesions of the Example 1 group were significantly improved compared with the model group, and there was no significant difference between Examples 2-5 and the model group; the lesion results of the jejunum were the same as the duodenal results: the lesion value of the blank control group was the lowest, and there was a significant difference between Example 1 and the model group; in terms of relative weight gain rate: the effect of the Example 1 group was better than that of the Example 2-5 groups.

[0121] Table 5: Test results data

[0122] Group-Drug Information Quantity (pcs) Average duodenal lesions Average jejunal lesion score Relative weight gain% Survival rate% Example 1 21 1.47 1.22 100.19 100.0 Example 2 20 2.16 1.95 94.23 95.2 Example 3 21 2.19 2.11 91.85 100.0 Example 4 21 2.16 2.22 91.88 100 Example 5 21 2.18 2.03 90.36 100 Lincomycin 21 1.39 1.19 96.98 100 Model control group 19 2.42 2.31 89.51 90.5 Blank control 21 1.42 1.21 100.00 100

[0123] 5 Conclusion

[0124] According to the results of intestinal lesions, Example 1 has a therapeutic effect on enteritis caused by Clostridium perfringens; Examples 2-5 have no obvious therapeutic effect on enteritis caused by Clostridium perfringens. This shows that Radix Psoraleae has an outstanding preventive and therapeutic effect on necrotic enteritis in chickens.

[0125] 3. Application of Dafengzi in regional clinical practice

[0126] Solve the economic losses caused by Eimeria toxicus and Clostridium perfringens mixed infection diseases in chicken farming.

[0127] Verification scheme

[0128] 1.1 Verification Purpose:

[0129] The therapeutic effect of Example 1 on necrotic enteritis in white-feathered broiler chickens was verified.

[0130] 1.2 Verification of drugs:

[0131] Example 1 is provided by Beijing Shengtai Technology Co., Ltd.

[0132] Lincomycin was provided by Beijing Shengtai Technology Co., Ltd.

[0133] 1.3 Verification Animals:

[0134] 38,000 white-feathered broilers were selected and raised in cages.

[0135] 1.5 Verification location:

[0136] A broiler farm in Faku County.

[0137] 1.6 Clinical symptoms:

[0138] The main symptoms are lack of energy, watery stools, red stools, Figure 1 .

[0139] 1.7 Autopsy symptoms:

[0140] The autopsy showed that the intestines were black, the intestinal wall was thin, the intestines were bloated, and there was red feces in the intestines. Figure 2 .

[0141] 1.8 Laboratory diagnosis:

[0142] Table 6. Pathogen detection results

[0143] Sample No. Test items Detection Methods Test results 5 Clostridium 16S PCR + 6 Clostridium 16S PCR +

[0144] Verification Method

[0145] Table 7. Drug use regimen

[0146] Drug Name Dosage Dongshe Lincomycin Administer according to product instructions for 4 consecutive days. 5 buildings Example 1 Calculated based on the total daily water intake, 1:500, concentrate on drinking water continuously for 4 hours, and use it continuously for 4 days. Building 6

[0147] At 24 days of age, 5 and 6 houses in the self-feeding farm were selected. House 5 was the lincomycin treatment group, and house 6 was the test group in Example 1. Three positions, front, middle and back, were selected from each group of chicken houses. The cages were fixed and photographed, and the number and proportion of tomato feces before and after the medication were counted.

[0148] Building 6, first observation point: see Figure 3 .

[0149] Group A (before medication): 16 piles in total, 9 piles of red stool, accounting for 56.3%;

[0150] Group B (first day of medication): 50 piles in total, 3 piles of red stools, accounting for 6%;

[0151] Group C (second day of medication): 30 piles in total, 1 pile of red stools, accounting for 3.3%;

[0152] Group D (third day of medication): 49 piles in total, 2 piles of red stools, accounting for 4.1%;

[0153] Group E (fourth day after medication): 32 piles in total, 1 pile of red stools, accounting for 3.1%.

[0154] Building 6, second observation point: see Figure 4 .

[0155] Group A (before medication): 19 piles in total, 7 piles of red stool, accounting for 36.8%;

[0156] Group B (first day of medication): 52 piles in total, 3 piles of red stools, accounting for 5.7%;

[0157] Group C (second day of medication): 35 piles in total, 2 piles of red stool, accounting for 5.2%;

[0158] Group D (third day of medication): 33 piles in total, 1 pile of red stools, accounting for 3%;

[0159] Group E (fourth day after medication): 34 piles in total, 1 pile of red stools, accounting for 2.9%.

[0160] Building 6, third observation point: see Figure 5 .

[0161] Group A (before medication): 24 piles in total, 4 piles of red stool, accounting for 16.7%;

[0162] Group B (first day of medication): 46 piles in total, 2 piles of red stools, accounting for 4.3%;

[0163] Group C (second day of medication): 42 piles in total, 2 piles of red stool, accounting for 4.7%;

[0164] Group D (third day of medication): 56 piles in total, 2 piles of red stools, accounting for 3.6%;

[0165] Group E (fourth day of medication): 49 piles in total, 1 pile of red stools, accounting for 4.1%;

[0166] Building 5, first observation point: see Figure 6 .

[0167] Group A (before medication): 27 piles in total, 5 piles of red stool, accounting for 18.5%;

[0168] Group B (first day of medication): 48 piles in total, 2 piles of red stools, accounting for 4.1%;

[0169] Group C (second day of medication): 52 piles in total, 2 piles of red stool, accounting for 3.8%;

[0170] Group D (third day of medication): 56 piles in total, 2 piles of red stools, accounting for 3.6%;

[0171] Group E (fourth day after medication): 36 piles in total, 1 pile of red stools, accounting for 2.8%.

[0172] Building 5, second observation point: see Figure 7 .

[0173] Group A (before medication): 36 piles in total, 4 piles of red stools, accounting for 11.1%;

[0174] Group B (first day of medication): 58 piles in total, 2 piles of red stools, accounting for 3.4%;

[0175] Group C (second day of medication): 34 piles in total, 4 piles of red stool, accounting for 11.7%;

[0176] Group D (third day of medication): 52 piles in total, 1 pile of red stools, accounting for 1.9%;

[0177] Group E (fourth day after medication): 37 piles in total, 1 pile of red stools, accounting for 2.7%.

[0178] Building 5, third observation point: see Figure 8 .

[0179] Group A (before medication): 38 piles in total, 5 piles of red stool, accounting for 13.2%;

[0180] Group B (first day of medication): 60 piles in total, 2 piles of red stools, accounting for 3.3%;

[0181] Group C (second day of medication): 40 piles in total, 3 piles of red stool, accounting for 7.5%;

[0182] Group D (third day of medication): 35 piles in total, 2 piles of red stools, accounting for 5.7%;

[0183] Group E (fourth day after medication): 53 piles in total, 1 pile of red stools, accounting for 1.8%.

[0184] Verify the results

[0185] 3.1 Fecal Observation Indicators

[0186] Table 8. Test of the 6th house

[0187] Age Number of red stools Percentage of red stool 23 days old 20 33.8％ 24 days old 8 5.4％ 25 days old 5 4.7％ 26 days old 5 3.6％ 27 days old 3 2.6％

[0188] Table 9. Test in the 5th house

[0189]

[0190]

[0191] in conclusion

[0192] Combining the photos and data, after 4 days of intensive drug treatment, the red feces gradually decreased. The daily red feces ratio of 6 houses dropped from 33.8% to 2.6%, and the daily red feces ratio of 5 houses dropped from 13.8% to 2.4%. The feces of the large group returned to normal, and the therapeutic effect was definite. Example 1 clinically showed the same effect of preventing and treating necrotic enteritis in chickens as the positive drug.

[0193] IV. The therapeutic effect of Radix Psoraleae on mixed infection of chicken intestinal coccidia and clostridium

[0194] Verification scheme

[0195] 1.1 Verification Purpose

[0196] The therapeutic effect of Example 1 on mixed infection of coccidia and clostridia in the small intestine of yellow chicken was verified.

[0197] 1.2 Verification of drugs

[0198] Example 1, provided by Beijing Shengtai Technology Co., Ltd.

[0199] 1.3 Validation Animals

[0200] 15,000 yellow-feathered broilers were selected and raised in flat conditions.

[0201] 1.4 Verification Location

[0202] A breeding farm in Kuiyang.

[0203] 1.5 Clinical symptoms

[0204] The main symptoms were lack of energy, slow feeding, 8 deaths, red feces, Fig. 9 .

[0205] 1.7 Autopsy symptoms

[0206] The autopsy showed yellow ulcers in the liver, thinning and bloating of some intestinal walls, and Fig.10 .

[0207] 1.8 Clinical diagnosis

[0208] Caused by mixed infection of intestinal coccidiosis and clostridium difficile;

[0209] The following table shows the medication regimen.

[0210] Table 10. Medication regimen

[0211] Drug Name Dosage How to use Observation indicators Example 1 1000ml to 500L water Calculate based on the total water intake throughout the day, concentrate on drinking water for 4 hours, and continue drinking water for 4 consecutive days. Stool status

[0212] At 45 days of age, the rats were orally administered the drug according to Example 1 for four consecutive days, which was divided into two observation points: A, B, C, D, and E respectively represented the 1st to 4th day of medication and the first day of drug withdrawal. The number of red feces per day was circled in red until the end of the experiment, and the proportion of red feces per day was calculated.

[0213] First observation point, see Fig.11 .

[0214] A: first day of medication; B: second day of medication; C: third day of medication; D: fourth day of medication; E: first day of stopping medication.

[0215] Group A had a total of 28 piles, including 9 piles of red feces, accounting for 32.1%; Group B had a total of 25 piles, including 9 piles of red feces, accounting for 36%; Group C had a total of 20 piles, including 7 piles of red feces, accounting for 35%; Group D had a total of 36 piles, including 7 piles of red feces, accounting for 19.4%; Group E had a total of 27 piles, including 3 piles of red feces, accounting for 11.1%.

[0216] The second observation point, see Fig.12 .

[0217] A: first day of medication; B: second day of medication; C: third day of medication; D: fourth day of medication; E: first day of stopping medication.

[0218] Group A had a total of 26 piles, including 11 piles of red feces, accounting for 42.3%; Group B had a total of 23 piles, including 6 piles of red feces, accounting for 26.1%; Group C had a total of 19 piles, including 4 piles of red feces, accounting for 21.1%; Group D had a total of 23 piles, including 4 piles of red feces, accounting for 17.4%; Group E had a total of 30 piles, including 3 piles of red feces, accounting for 10%.

[0219] Verify the results

[0220] The experimental results are as follows.

[0221] Table 11. Fecal observation indicators

[0222] Age Red feces quantity Red feces percentage 45 days old 20 37.3％ 46 days old 15 31.3％ 47 days old 11 28.2％ 48 days old 11 18.9％ 49 days of age (drug withdrawal) 6 10.5％

[0223] in conclusion

[0224] For hemorrhagic enteritis in yellow-feathered broilers caused by mixed infection of coccidia and clostridia in summer and autumn, after 4 days of intensive drug treatment, the red feces were greatly reduced, and the daily percentage of red feces dropped from 37.3% to 10.5%, with obvious drug efficacy, indicating that Example 1 has obvious therapeutic effect on hemorrhagic enteritis in chickens caused by mixed infection of coccidia and clostridia in the small intestine.

Claims

1. Use of Fructus Caryophylli in preparing medicine for preventing or treating chicken coccidiosis.

2. The use according to claim 1, characterized in that The chicken coccidiosis is chicken Eimeria coccidiosis.

3. The use according to claim 2, characterized in that: The chicken coccidiosis disease is Eimeria tenella, Eimeria acervulina, Eimeria maxima, Eimeria toxicophila, Eimeria brucei, Eimeria mitis and / or Eimeria precocious.

4. The use according to claim 3, characterized in that Chicken coccidiosis is a chicken disease caused by Eimeria toxicans.

5. Use of Fructus Caryophylli in preparing medicine for preventing or treating necrotic enteritis in chickens.

6. The use according to claim 5, characterized in that Necrotic enteritis disease in chickens is caused by Clostridium perfringens.

7. Use of Fructus Caryophylli in preparing medicine for preventing or treating chicken coccidiosis and chicken necrotic enteritis mixed disease.

8. The use according to any one of claims 1 to 7, characterized in that The radix spatholobi is a medicinal material powder or a radix spatholobi extract.

9. The use according to claim 8, characterized in that The extract of Fructus Caryophylli is water extract or alcohol extract.

10. The use according to any one of claims 1 to 7, characterized in that: The medicated oil is combined with other medicines to prepare medicines.

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