551 results about "Salmonella" patented technology

The invention discloses a plant lactobacillus with a bacteriostatic effect and application of the plant lactobacillus. The lactobacillus plantarum is named as lactobacillus plantarum C07, the preservation number is CCTCC NO: M20242086, the preservation unit is China Center for Type Culture Collection, the preservation time is September 26, 2024, and the preservation address is Wuhan University, Wuhan University, No. 299, eight road, Wuchang District, Wuhan City, Hubei Province. The strain can produce lactic acid and indole-3-lactic acid at high yield, has a very strong antibacterial effect on multiple putrefying and pathogenic microorganisms such as staphylococcus aureus, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella and mould, and is a broad-spectrum antibacterial microorganism.

The invention relates to the field of microorganisms, and discloses lactobacillus plantarum with an antibacterial effect and application of the lactobacillus plantarum, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) No.33151. The strain provided by the invention has relatively strong cholate resistance, gastric acid resistance, acid production and H2O2 production capabilities, and has a relatively strong bacteriostatic effect on common pathogenic bacteria such as staphylococcus aureus, candida albicans, escherichia coli, salmonella and helicobacter pylori; the compound can inhibit the activity of urease, interfere helicobacter pylori colonization and the like to inhibit helicobacter pylori infection or reduce the symptoms of helicobacter pylori infection and reduce the disease degree of mice with colitis, can be used for preparing microbial preparations, and has the potential of preparing drugs for preventing and / or treating diseases caused by helicobacter pylori infection.

The invention discloses an attenuated salmonella typhimurium EN-VNP (Enhanced Natriuretic Peptide) strain as well as a preparation method and application of the attenuated salmonella typhimurium EN-VNP strain. Comprising the following steps: on the basis of a salmonella VNP20009 strain, flagellin genes fliC and fljB, a phosphoethanolamine transferase gene eptA, a 4-amino-4-deoxy-L-arabinose transferase gene arnT and phosphotransferase yeiU are sequentially knocked out or silenced, and a strain is formed after a phosphotransferase gene lpxE is knocked in or overexpressed, so that the attenuated salmonella typhimurium EN-VNP strain is prepared. According to the attenuated salmonella typhimurium EN-VNP with low toxicity and the T cell adjuvant effect and an EN-OMV antigen delivery system prepared from the attenuated salmonella typhimurium EN-VNP, an improved strain does not contain flagellin, an LPS molecular structure is changed, the attenuated salmonella typhimurium EN-VNP has the low toxicity and the T cell adjuvant effect, and normal multiplication culture can be achieved.

A breast milk-derived Limosilactobacillus fermentum MSJK0025 capable of improving the intestinal flora condition and the use thereof. The breast milk-derived Limosilactobacillus fermentum MSJK0025 is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 26426. The breast milk-derived Limosilactobacillus fermentum is a new strain isolated from breast milk of healthy puerperae, and is referred to as Limosilactobacillus fermentum MSJK0025. The strain has a good antibiotic sensitivity and stable inheritance of traits, has a good tolerance to acid, bile salts and artificial gastric / intestinal fluid, and has a fast growth rate and good safety. Furthermore, the strain has a strong intestinal colonization capability and can effectively inhibit intestinal pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Candida albicans, but has no inhibitory effects on intestinal probiotics such as Lactobacillus and Bifidobacterium.

The invention discloses a method for displaying foreign protein by utilizing salmonella and application of the method, and belongs to the technical field of biological medicine. According to the invention, the SpyCatcher and an outer membrane protein Lpp-OmpA gene are fused and are expressed in salmonella, a recombinant strain is constructed, and the SpyCatcher is displayed on the surface of bacteria; the foreign protein gene and the SpyTag gene are fused and subjected to prokaryotic expression purification, and recombinant foreign protein is obtained; after the recombinant foreign protein and the recombinant salmonella are incubated, the foreign protein can be anchored on the surface of the salmonella through a SpyCatcher-SpyTag covalent bond. When the foreign protein is an antigen protein, the foreign protein is displayed on the surface of the attenuated bacterial vector, and a live vector vaccine can be prepared. The invention provides a novel universal antigen display method, overcomes the limitation of the traditional antigen display method on the protein size and conformation, and has a good application prospect in live vector vaccine construction.

The invention discloses swine bacillus velezensis YB-X-733 and application thereof, and relates to the technical field of microorganisms. The swine-derived bacillus velezensis YB-X-733 is preserved in the China General Microbiological Culture Collection Center on September 24, 2024, the preservation number is CGMCC No.32057, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the swine-derived bacillus velezensis YB-X-733 is classified and named as bacillus velezensis. The swine bacillus velezensis YB-X-733 disclosed by the invention has a good inhibition effect on escherichia coli, staphylococcus aureus and salmonella, and can be used for preparing a piglet inflammation resisting product. The swine-origin bacillus velezensis YB-X-733 disclosed by the invention can be used for obviously improving the metabolic capability of weaned piglets and improving the physiological status of the piglets; the immunocompetence of weaned piglets is improved, and inflammation is reduced; the microbial flora in the intestinal tracts of the weaned piglets can be obviously improved, the growth of harmful bacteria is inhibited, and the growth of beneficial bacteria is promoted.

The invention relates to the technical field of hydrogel, in particular to a preparation method and application of EGCG-mediated hydrogel, a lysozyme solution is heated under the acidic condition to form lysozyme amyloid fibrils LAFs, EGCG is dissolved in a buffer solution and mixed with the LAFs solution obtained in the step 1 in proportion to form an LAFs-EGCG compound, and the EGCG-mediated hydrogel is prepared. The chitosan CS is dissolved in an acetic acid buffer solution, glutaraldehyde is added to serve as a cross-linking agent, the mixture is mixed with the LAFs-EGCG compound obtained in the step 2 and stirred, the composite hydrogel is formed, the concentration of EGCG is 0.2-1.0 wt%, the concentration of LAFs is 5 wt%, the concentration of chitosan is 2 wt%, the pH value of the lysozyme solution in the step 1 is 2.0, the heating temperature is 77 DEG C, the heating time is 10 hours, the concentration of EGCG in the step 2 is 0.4-0.8 wt%, and the pH value of the lysozyme solution in the step 2 is 1.5-2.5. In the step 3, the concentration of glutaraldehyde is 2%, the storage modulus G'of the hydrogel is 325-480 Pa, the hardness is 2176.71 g, and the bacteriostasis rate on staphylococcus aureus and salmonella exceeds 95%.

The invention discloses a polypeptide drug for resisting gram-negative bacteria and application, and belongs to the technical field of biological medicine. The MIC of the antibacterial peptide obtained through screening on escherichia coli, salmonella typhimurium, pseudomonas aeruginosa, klebsiella pneumoniae, shigella sonnei and stenotrophomonas maltophilia is 16 [mu] g / mL, 2 [mu] g / mL, 16 [mu] g / mL, 4 [mu] g / mL, 2 [mu] g / mL and 4 [mu] g / mL respectively, and the antibacterial peptide has broad-spectrum antibacterial performance. The hemolytic activity and toxicity of the compound are lower than therapeutic concentration threshold values, and the compound can be used for preparing drugs or medical products for treating multi-drug-resistant bacterium infection.

The invention is applicable to the technical field of polypeptides, and provides a novel antibacterial peptide GG25 and application thereof, and the amino acid sequence of the antibacterial peptide GG25 is as shown in SEQ ID No.1. The molecular weight of the novel antibacterial peptide GG25 is 2890.52 Da, and the novel antibacterial peptide GG25 has antibacterial activity on escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, enterococcus faecalis, salmonella typhimurium, methicillin-resistant staphylococcus aureus, staphylococcus aureus, staphylococcus epidermidis, listeria monocytogenes and bacillus subtilis; the hemolytic activity is low, the cytotoxicity is small, and the escherichia coli and methicillin-resistant staphylococcus aureus are not easily induced to generate drug resistance; in addition, the gelatin chitosan food coating containing GG25 also has a good beef preservation effect, and can be applied to the fields of food, animal husbandry, medicine and the like.

The invention discloses a chicken infectious anemia virus subunit vaccine, and belongs to the technical field of animal disease vaccines. The invention provides a chicken infectious anemia virus subunit vaccine. Active ingredients of the chicken infectious anemia virus subunit vaccine are recombinant fusion protein of chicken infectious anemia virus VP1 protein B cell epitope and salmonella typhimurium flagellin. The B cell epitope of the chicken infectious anemia virus VP1 protein is obtained by connecting B cell antigen epitopes contained in the chicken infectious anemia virus VP1 protein through a connecting peptide. The salmonella typhimurium flagellin is obtained by deleting a hypervariable region of the salmonella typhimurium flagellin FliC. The fusion expression protein provided by the invention can induce generation of a specific IgG antibody after one-day-old SPF chicken, and after immunization for 28 days, immunized chicken flocks can resist challenge of chicken infectious anemia virus virulent strains, and 70% of challenge chicken flocks are protected without disease attack. The invention has great potential for clinical prevention and control of chicken infectious anemia virus infection.

The invention discloses a colorimetric-fluorescent dual-signal salmonella detection method based on Ag-MoS2 nano-enzyme. The method is characterized by comprising the following steps: mixing a single strand S1, a single strand S2, a single strand S3, a single strand S4, a salmonella aptamer and cDNA (complementary deoxyribonucleic acid) to prepare a multivalent aptamer competitive probe solution; respectively dissolving a hairpin probe H1 and a hairpin probe H2 in PBS (Phosphate Buffer Solution); a step of preparing an Ag-MoS2 nano enzyme composite material; the method comprises the following steps: mixing and incubating a to-be-detected salmonella solution and a multivalent aptamer competitive probe, adding the Ag-MoS2 nano-enzyme composite material for performing CHA amplification reaction, then adding an H2O2 solution and a TMB solution for performing peroxidase catalytic reaction, respectively performing colorimetric method and fluorescence method detection, and calculating to obtain the salmonella concentration in the to-be-detected solution. The kit has the advantages of simplicity, rapidness, high sensitivity, strong specificity, high accuracy and real-time sterilization after detection.

A specific protein fragment capable of representing salmonella as an antigen is obtained through screening and experimental verification, a mouse is immunized with the specific protein fragment as the antigen, and hybridoma cells capable of stably secreting monoclonal antibodies are obtained through a cell fusion technology. And finally, the anti-salmonella monoclonal antibody with high titer and good specificity is prepared. Similarly, the specific protein fragment is used for immunizing a rabbit to obtain the anti-salmonella polyclonal antibody. On the basis of a mouse monoclonal antibody and a rabbit polyclonal antibody, a salmonella colloidal gold immunochromatography detection method is established, the detection method is good in specificity, and a rapid, simple and convenient detection means is provided for detection of salmonella in food.

The invention discloses an antibacterial peptide fragment intercepted from chitinase IDGF4 protein and application of the antibacterial peptide fragment in preparation of antibacterial drugs. According to the invention, short peptide fragments in chitinase IDGF4 protein are screened, three candidate peptides are obtained through chemical synthesis, the antibacterial performance of the candidate peptides is systematically evaluated, and the evaluation result shows that the peptide fragment with the amino acid sequence as shown in SEQ ID No.2 can effectively inhibit the growth of salmonella and staphylococcus aureus under the concentration of 1mg / mL; the antibacterial effect of the compound is confirmed in inhibition zone experiments, enzyme-labeled turbidimetry and growth curve monitoring, and the compound shows stable and remarkable antibacterial activity and can be used as a novel antibacterial molecule; the invention provides a new candidate sequence for developing the antibacterial peptide with high efficiency and low drug resistance risk, and has application potential in the aspect of preparing clinical antibacterial drugs.

The invention relates to an aerobic denitrification halomonas tianxiuensis LHT6, the preservation number of which is CGMCC No.32312. The strain can tolerate salinity, heavy metal ions and antibiotic stress under the condition of low carbon nitrogen ratio to realize efficient denitrification, and has application significance in actual treatment of water bodies such as aquaculture wastewater and pharmaceutical wastewater.

The invention relates to the technical field of medicines, in particular to an antibacterial composition as well as a preparation method and application thereof. The invention provides an antibacterial composition. The antibacterial composition is prepared from the following components in parts by mass: 0.039 to 0.3125 part of SU3327 and 62.5 to 500 parts of citric acid. The combined use of the SU3327 and the citric acid can effectively kill and / or inhibit vibrio parahaemolyticus, escherichia coli, salmonella, acinetobacter baumannii, staphylococcus aureus, klebsiella pneumoniae and staphylococcus pseudointermediates, and the use amount of the SU3327 in the medicament for treating bacterial infection is greatly reduced; and the compound shows extremely high application value in the process of preparing medicines for treating bacterial infection.

The invention belongs to the technical field of biology, and discloses a preparation method of fusion protein of salmonella typhimurium flagellin and Seneca virus antigen. Escherichia coli Rosetta (DE3) is selected as a host, a salmonella typhimurium flagellin gene and a Seneca virus antigen gene are fused and are cloned to a pET-28a (+) vector after codon optimization, the obtained fusion protein keeps the flagellin adjuvant activity, the solubility and the expression level of VP2 are improved, the yield can reach 40 mg / L, and the fusion protein can be used for preparing a recombinant vector. The expression and purification process cost is greatly reduced. The fusion protein provided by the invention can activate a TLR5 pathway and mouse macrophages, is simple in production process and low in cost, and has important significance on research and production of Seneca virus subunit vaccines.

The invention provides a poultry probiotic preparation prepared by fermenting selenium-resistant duck-origin lactobacillus plantarum. The poultry probiotic preparation is prepared by inoculating duck-origin lactobacillus plantarum with high selenium ion loading capacity into a culture medium and fermenting, the duck-origin lactobacillus plantarum with high load of selenium ions has the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.30182. The high-load selenium duck-origin lactobacillus plantarum obtained by domesticating and screening by adopting a method for gradually increasing the concentration of selenium ions in a culture medium contains small peptides, small molecular organic acids, digestive enzymes and the like. As the antibacterial peptide is rich in targeted antibacterial peptide, the antibacterial peptide has an obvious inhibition effect on harmful bacteria such as escherichia coli, salmonella, riemerella anatipestifer and the like. Other bioactive substances such as digestive enzyme contained in the feed can promote the growth and development of poultry and improve the product quality.

The invention provides a fluorenyl alcohol derivative which can be used as an antibiotic adjuvant with a broad-spectrum synergistic effect. According to the compound, bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are taken as targets, and a series of fluorenyl alcohol derivatives targeting the bacteria outer membrane asymmetric protein MlaC and phospholipase A1 PldA are obtained through screening. The preferable compound WTU-15 can improve the antibacterial activity of antibiotics such as polymyxin, cefepime, tetracycline and the like on negative bacteria such as sensitive and drug-resistant escherichia coli, salmonella, klebsiella pneumoniae, acinetobacter baumannii and the like, and can also improve the in-vivo treatment effect of cefepime on mice infected by the drug-resistant acinetobacter baumannii. And the visceral organs of the mice survived after administration have fewer bacteria, and the mice are better after being healed. Normal physiological and visceral organ functions of the mouse are not affected by single and continuous administration of the WTU-15.

The invention discloses application of a method for enhancing the treatment effect of salmonella bacteriophage, and relates to the technical field of microorganisms. According to the method, bacteriophage is added into salmonella for in-vitro culture, after continuous subculture, the salmonella is dropwise added to an SS plate containing the bacteriophage, and resistant bacteria of the bacteriophage are screened. The split bacteriophage is screened aiming at the resistant bacteria of the bacteriophage, and the split bacteriophage and the initial bacteriophage form a bacteriophage cocktail formula, so that the salmonellosis can be well prevented and treated, and the generation of the resistant bacteria is delayed. The method has the beneficial effects that compared with the existing method for screening the bacteriophage-resistant bacteria through a double-layer plate method, the method is more convenient, the operation is simple, and the screening efficiency of the bacteriophage-resistant bacteria is greatly improved. The bacteriophage cocktail contains the bacteriophage for cracking the resistant bacteria, so that the treatment effect of the bacteriophage is improved, and the practical application scene is quite high.

The invention belongs to the technical field of microorganisms, and relates to a selenium-rich bacillus velezensis with high tolerance and application of the selenium-rich bacillus velezensis. The invention relates to a selenium-rich bacillus velezensis 915-1 with strong tolerance, which is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is (GDMCC No: 64021). The bacillus velezensis 915-1 can effectively inhibit growth of escherichia coli, salmonella and staphylococcus aureus after inoculation as first inoculation culture bacteria, and can be used as probiotics or drugs for colonization in intestinal tracts to play roles in inhibiting pathogenic bacteria and benefiting life. The bacillus velezensis is applied to production of organic selenium. The bacillus velezensis can absorb, convert and utilize sodium selenite, the sodium selenite is enriched into thalli to be converted into organic selenium, the content of the organic selenium is increased along with increase of the number of cells, and the bacillus velezensis can be used for production and preparation of the organic selenium.

The invention relates to a preparation method and application of recombinant canine lactoferrin and peptide thereof, aiming at codon preference of host cells, canine lactoferrin genes are optimized, the full length of canine lactoferrin and the lactoferrin peptide are successfully expressed by virtue of an escherichia coli expression system, and the recombinant canine lactoferrin and the peptide thereof are identified by virtue of a minimum inhibitory concentration experiment. It is proved that the canine lactoferrin and the peptide thereof have the inhibiting capacity on pathogenic bacteria such as staphylococcus aureus, salmonella typhimurium and staphylococcus pseudointermedius, and the antibacterial activity MIC of the canine lactoferrin on the staphylococcus aureus, the salmonella typhimurium and the staphylococcus pseudointermedius is 1.5 mg / mL; the antibacterial activity MIC of the dog lactoferrin peptide to staphylococcus aureus is equal to 0.75 mg / mL, and the dog lactoferrin peptide shows high antibacterial ability.

The invention belongs to the field of biology, relates to a salmonella gene detection technology, and discloses a one-pot detection method combining LAMP (loop-mediated isothermal amplification) and CRISPR / BrCas12b mediated nucleic acid molecule detection. The method comprises the following steps: designing an LAMP primer, a small guide RNA (Ribonucleic Acid) and a transcription template overlapping PCR (Polymerase Chain Reaction) primer, performing loop-mediated isothermal amplification, constructing CRISPR / Cas shearing recognition (Cas-LAMP), constructing a single-tube one-pot reaction system of the CRISPR / Cas shearing recognition, detecting the specificity and sensitivity of Cas-LAMP amplification, and the like. In order to solve the technical problem that amplification and shearing recognition cannot be carried out synchronously, the invention provides a Cas-LAMP technology capable of linking two-step reaction of amplification and shearing recognition, and accurate, sensitive and rapid detection of nucleic acid molecules is realized in an in-vitro single reaction tube system. Compared with a traditional nucleic acid molecule detection method, the method has the following advantages that one-pot detection can be directly carried out without step-by-step operation, high sensitivity and strong specificity are achieved, and rapid field detection can be achieved. The method provides technical support for conventional nucleic acid detection, and has a wide field instant detection application prospect.

The invention discloses a bacteriophage lyase and application thereof, and particularly relates to a bacteriophage lyase with an amino acid sequence as shown in SEQ INNO.2. The bacteriophage lyase can inhibit the growth of escherichia coli, staphylococcus and salmonella, can be used as an antibacterial substance in splitting gram-negative bacteria and can be used for preparing drugs for resisting gram-negative bacteria. The lyase can be used independently or compounded with other substances, the use concentration of antibiotics can be reduced by combining the lyase with the antibiotics, toxic and side effects (such as renal toxicity of polymyxin) of drugs are reduced, and meanwhile, generation of bacterial drug resistance is delayed. After the lyase and the chitosan are combined for use, remarkable antibacterial activity is generated, the antibacterial effect can be achieved without pre-treatment on bacteria, and the practicability is improved.

The invention provides a salmonella bacteriophage and a preparation method and application thereof, the salmonella bacteriophage is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 10, 2021, and the preservation number is CGMCC No.23090. The invention also provides a preparation method of the salmonella bacteriophage, which comprises the following steps: carrying out combined fermentation on salmonella host bacteria from multiple species and salmonella bacteriophage seeds to obtain fermentation liquor, and centrifugally filtering the fermentation liquor to obtain the salmonella bacteriophage. According to the invention, multi-strain combined fermentation of bacteriophages of salmonella of different provenances and species is realized. According to the combined fermentation process, combined fermentation of multiple strains of bacteriophages of salmonella of different provenances and species is achieved, it is guaranteed that the bacteriophage of each strain of host bacteria can be successfully fermented, and the production cost and the production cycle of bacteriophage fermentation of a single strain of salmonella are reduced.

The invention belongs to the technical field of cleaning of poultry eggs, and discloses a special cleaning agent for poultry eggs as well as a preparation method and application thereof. The special cleaning agent for poultry eggs comprises the following components: linear alkylbenzene sulfonate, fatty alcohol polyoxyethylene ether sodium sulfate, cocoanut fatty acid diethanolamide, sodium silicate, sodium tripolyphosphate, sodium carbonate, sodium chloride, sodium hypochlorite, a defoaming agent, an enzyme preparation and water. The cleaning agent is used for cleaning fresh poultry eggs, dirt on the surfaces of the poultry eggs can be rapidly removed, the dirt can be prevented from being adhered again, the sterilization rate on escherichia coli, staphylococcus aureus and salmonella reaches 95% or above, meanwhile, the influence on the strength of the poultry eggs can be reduced to the maximum extent, and the breakage rate of the poultry eggs is kept at the low level.

The process comprises providing a recombinant vaccine construct, wherein said construct comprises genetically modified Lactobacillus plantarum NC8 as a live vector; modifying the genetic structure of said Lactobacillus plantarum NC8 to express conserved Salmonella antigens, including PagN, SopE2, and FliC, anchored by a ptrk 892 backbone with a constitutive promoter, phosphoglycerate mutase (PGM); incorporating Signal Lp_2145 and cAM12 Anchor sequences into said genetic construct to enhance surface expression of recombinant proteins on Lactobacillus plantarum NC8; administering said recombinant vaccine orally to poultry, leveraging the probiotic properties of Lactobacillus plantarum NC8 for effective colonization of the poultry gastrointestinal tract; inducing a prolonged and intensified immune response by ensuring sustained high-level expression of target antigens through the utilization of the robust constitutive promoter, phosphoglycerate mutase (PGM); and optimizing immunogenicity through the surface expression of recombinant proteins on Lactobacillus plantarum NC8, fostering a robust and precisely targeted immune response.

The invention discloses a fusion antibacterial peptide BMAP18-BSN37 and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the fusion antibacterial peptide BMAP18-BSN37 is as shown in SEQ ID NO. 1, and the amino acid sequence of the fusion antibacterial peptide BMAP18-BSN37 is as shown in SEQ ID NO. 2. The fusion antibacterial peptide BMAP18-BSN37 gene is inserted into a pNZ8148 expression vector to construct a recombinant expression vector pUBB, so that protein aggregation or degradation in cells can be avoided, and the survival rate of strains and the stability of protein expression are improved. The method comprises the following steps: transferring a plasmid pUBB into a lactic acid bacteria NZ9000 strain through electrotransformation, and screening a recombinant lactic acid bacteria strain capable of expressing a fusion protein BMAP18-BSN37 to obtain a recombinant strain NZ-BB; the recombinant lactic acid bacteria NZ-BB strain shows a remarkable prevention effect in an animal infection model, and can effectively reduce bacterial colonization and relieve pathological injury; the Salmonella strain has the potential of being used as an animal feed additive, can reduce the use of antibiotics and chemicals, slow down the development of bacterial drug resistance, improve animal immunity and slow down intestinal inflammation caused by salmonella, and provides support for effectively preventing the health of livestock and poultry.

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The invention belongs to the technical field of micromolecular chemiluminescent materials, and particularly relates to an esterase activated chemiluminescent probe for microbiological detection as well as preparation and application of the esterase activated chemiluminescent probe. The chemiluminescent probe disclosed by the invention is a compound prepared from an adamantane-dioxetane skeleton (AD-CL), and the chemiluminescent probe comprises two compounds which are named as ACCL or BUCL; the compound with the structure can be activated by esterase to generate chemiluminescence. The esterase for microbial detection comprises enzymes which are contained in escherichia coli, staphylococcus aureus and salmonella and can hydrolyze acetyl ester groups, butyryl ester groups, hexanoyl ester groups or capryloyl ester groups. The chemiluminescent probe can be used for responding to various esterases in vitro, and can also be used for microbiological detection and clinical disease diagnosis.

The invention discloses a method for rapid broad-spectrum detection of salmonella, and relates to the technical field of bacterial detection. Comprising the following steps: (1) preparing a labeled SERS probe; (2) preparing a Fe3O4 (at) Au (at) Ag magnetic composite material; (3) combining Fe3O4 (at) Au (at) AgNPs with Ag (at) 4MBA (at) AuNPs to construct an aptamer sensor; and (4) carrying out SERS detection on the salmonella. According to the novel surface enhanced Raman scattering (SERS) aptamer sensor constructed on the basis of noble metal nanoparticles and a magnetic composite material, according to the inverse proportion relation that the higher the salmonella concentration is, the weaker the Raman signal is generated according to the principle of a competition law, the ultrasensitive rapid field detection is carried out on different salmonella.