33 results about "Salmonella sps" patented technology

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses preparation and application of Clostridium butyricum and a live Clostridium butyricum preparation; Clostridium butyricum LXKJ-1 is collected in China Center for Type Culture Collection on 24 March, 2016 under CCTCC NO: M201613. A seed medium, fermentation medium formulation, culture conditions for Clostridium butyricum and a production of a preparation of live Clostridium butyricum are also disclosed. The Clostridium butyricum provided herein is tolerant to acids, bases and high temperature, is highly capable of producing butyric acid, can inhibit animal pathogens, such as Escherichia coli, Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogenes, and Clostridium perfringens, and can prevent diarrhea in livestock and poultry due to Escherichia coli, Salmonella and Clostridium perfringens, improving intestinal flora balance, promoting the growth of livestock and poultry, relieving constipation in sows, improving egg weight for layers, improving eggshell quality for layers, and decreasing egg-feed ratio.

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The invention discloses a salmonella phage LPSE34. The preservation number of the salmonella phage LPSE34 is CCTCC NO 2018121. The phage is a broad-spectrum salmonella phage and can crack drug-tolerant salmonellas, it is identified that the phage belongs to myoviridae, and the phage is named as LPSE34. The phage LPSE34 is stable in titer at the pH of 4-12 and the temperature of 30-60 DEG C. The invention further discloses application of the salmonella phage in food, especially in chicken and ham systems; and in addition, the invention further discloses a compound edible coating film formed bytaking the phage as an antibacterial substance to be combined with sodium alginate, and compared with antibiotics and chemical preservatives, the phage cannot affect the texture and flavor of food andhas the characteristics of high specificity, no residue and safety. The salmonella phage LPSE34 can serve as a bacteriocidal substance/the antibacterial substance to control pollution of food-borne pathogens to the food and is a very promising biological agent for guaranteeing the food safety.

The invention discloses a detection method for salmonella enteritidis and a detection kit. A salmonella enteritidis specific aptamer is taken as a molecular recognition element, a stem-loop structure nucleic acid H1 is opened under the action of salmonella enteritidis to undergo interaction with a stem-loop structure nucleic acid H2 so as to form partial double-stranded DNA, which includes endonuclease recognition sequences, H2 is incised under the action of endonuclease to release G basic group-rich nucleotide sequences. Under the action of Hemin, a G tetramer structure with similar HRP catalytic activity is formed, can achieve catalytic oxidation of chromogenic reaction to turn colourless substrate into blue. The method provided by the invention has high sensitivity, has a detection limit is 10 cfu/mL, also has good specificity, and other common bacteria have no influence on detection. The whole detection process can be completed at room temperature. The method has the advantages of simple operation, economical efficiency and cheapness, etc. The detection result can be directly observable, and no detecting instrument is sneeded. The method can detect living bacteria directly, and has no need for lysis of bacteria.

The present invention is drawn to multivalent Salmonella enterica serovar conjugate vaccines comprising conjugates of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi, S. Paratyphi A and optionally S. Paratyphi B, wherein the conjugates comprise a hapten antigen and a carrier antigen, wherein at least one of the hapten antigens or carrier antigens is characteristic of the Salmonella enterica serovar. The present invention also provides Salmonella enterica serovar reagent strains to produce the multivalent conjugate vaccines and attenuated Salmonella enterica serovars for use as vaccines.

The invention relates to a fowl salmonella gallinarum attenuated isolated strain and application thereof. The isolated strain is preserved in the China General Microbiological Culture Collection Center, Beijing, China, on July 2, 2018, has a preservation number of CGMCC No.16049, and is classified and named as Salmonella Gallinarum. The strain is attenuated to chicken of different ages, is apathogenic, and can be used as a strain vector for preventing livestock salmonella gallinarum infection and preventing infectious diseases of livestock (chicken). The fowl salmonella gallinarum attenuated isolated strain provides data reference to evaluating the toxicity of salmonella attenuated vaccine strains and potential as the strain vector of livestock (chicken) live vaccines.

The invention discloses a salmonella bacteriophage and bacteriophage anti-bacterium composition and an application thereof. A bacteriophage is salmonella enteritidis bacteriophage LPSE1 and salmonella typhimurium bacteriophage LPST10, and both the salmonella enteritidis bacteriophage LPSE1 and the salmonella typhimurium bacteriophage LPST10 have excellent inhibiting effect on salmonellas and good tolerance for pH (potential of hydrogen) and temperature. Application examples of the salmonella bacteriophage to food systems verify that the bacteriophage has excellent effect when being used for bacteriostatic agents. The salmonella enteritidis bacteriophage LPSE1 and the salmonella typhimurium bacteriophage LPST10 have synergistic effects after being mixed and have more beneficial inhibiting effect as compared with a single bacteriophage.

The invention discloses a lactobacillus salivarius strain and application thereof. The lactobacillus salivarius strain (with number of HVRIC4) is obtained by carrying out bacteria reproduction and enrichment on wild chicken manure in an MRS liquid selective medium and carrying out isolated culture and inoculation and purification. The microbial collection number of the strain is CGMCC No.6786. Lactobacillus salivarius has stronger acid and bile salt resistance. Antibacterial experiments in vitro indicate that the strain has stronger antibacterial effects on Gram-negative escherichia coli, salmonella and Gram-positive staphylococcus, shows a broad-spectrum antibacterial effect, has the effect of promoting growth of SPF (specific pathogen free) chickens, can effectively resist infection with salmonella and avian infectious laryngotracheitis viruses when being fed to the SPF chickens and has the effect on effectively protecting the SPF chickens.

The invention relates to a Listeria monocytogenes monoclonal antibody hybridoma cell strain and an application thereof and belongs to the technical field of food safety immunological detection. According to the invention, a complete antigen of a specific polypeptide of Listeria monocytogenes is uniformly mixed with an equal volume of Freund adjuvant (Sigma), BALB/c mice are immunized by subcutaneous injection, after the BALB/c mice are immunized for four times, spleen cells of immunized mice are fused with mice myeloma cells of immunized mice by virtue of a PEG method, an indirect ELISA screening and three times of subcloning are carried out to obtain a monoclonal cell strain A. The monoclonal antibody secreted by virtue of the monoclonal cell strain A has cross reaction with different Listeria monocytogenes culture supernatants and no cross reaction with listeria except Listeria monocytogenes, E. coliO 157, Salmonella and Campylobacter jejuni and thus the Listeria monocytogenes monoclonal antibody hybridoma cell strain can be used in the specific detection of Listeria monocytogenes in a food.

The invention relates to the field of food microorganisms, in particular to lactobacillus kefiranofaciens CICC 6278 and application of the lactobacillus kefiranofaciens to Xinjiang characteristic chili fermentation. Nitrite can be efficiently and quickly degraded by the lactobacillus kefiranofaciens, the lactobacillus kefiranofaciens is low in biogenic amine production, and pathogenic microorganisms can be inhibited by the lactobacillus kefiranofaciens. A bacterial strain of the lactobacillus kefiranofaciens is preserved in the China General Microbiological Culture Collection Center, and a preservation number of the bacterial strain is CGMCC 14017. The lactobacillus kefiranofaciens and the application have the advantages that the nitrite degradation rate can reach 94.8% if the bacterial strain is cultured in improved MRS liquid culture media for 24 h, and the nitrite can be completely degraded if the bacterial strain is cultured for 36 h; the bacterial strain comprises negative amino acid decarboxylase, and the pathogenic microorganisms including Escherichia coli, staphylococcus aureus, listeria monocytogenes and salmonella can be inhibited; the bacterial strain can be used for Xinjiang characteristic chili fermentation, the content of nitrite in obtained fermented chili is 1.06 mg/kg, the content of biogenic amine in the fermented chili is 5.62 mg/kg, and accordingly the edible safety of the fermented chili can be improved.

The invention discloses a salmonella bacteriophage nano magnetic bead conjugate and its enrichment and separation kit. The conjugate is a complex of a salmonella bacteriophage coupled with a carboxylmagnetic bead, wherein the salmonella bacteriophage is Salmonella Typhimurium bacteriophage LPST10, and a preservation number is CCTCC NO: M 2016473. The bacteriophage of the Salmonella bacteriophagenano magnetic bead conjugate of the invention can specifically recognize and infect bacteria, and has the characteristics of good specificity and small molecular weight. The kit is capable of rapidlyand sensitively separating and enriching salmonella.

The invention discloses a construction method of a specificity salmonella H:z29 diagnostic serum engineering strain. The construction method is characterized in that a modern molecular biological technology is used for transforming a conventional multi-clone antibody preparation system, and surface flagellar antigen synthetic gene deleted host bacteria are constructed; surface flagellar antigen synthetic genes of different pathogenic microorganisms are cloned to carriers suitable for expression, and the carriers are transferred to the antigen gene deleted host bacteria so as to obtain a series of engineering strains having the same genetic background and containing different pathogenic microorganism specificity surface flagellar antigen genes. The technology system can be used for constructing a special antibody library comprising a plurality of salmonellae. The antibody library can be used for supplementing existing anti-serum types and meanwhile can provide basis for the application of other immunology technologies.

The invention discloses a real-time fluorescence PCR based method for rapid screening of pathogenic microorganisms in cucumber. The method combines the traditional bacterial culture method, uses water-boiling method to extract salmonella, staphylococcus aureus, Escherichia coli O157:H7 and Listeria monocytogenes in selectively enriched cucumber, and applies real-time fluorescence PCR technology to screen the four food-borne pathogenic bacteria quickly. The DNA extraction technique adopted by the invention can complete the whole process of sample DNA extraction, and the real-time fluorescence PCR technique can whole process identification of a sample within 90min, therefore the method provided by the invention has the advantages of high efficiency, quickness, real-timeness, simple operation and the like, and provides technical support for rapid screening of food-borne pathogenic bacteria in fresh fruit and vegetable cucumber.