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Fowl salmonella gallinarum attenuated isolated strain and application thereof

A technology of Salmonella and isolates, applied in the field of attenuated live vaccine candidate strains of poultry bacteria, can solve the problem of difficult control of Salmonella infection in chicken flocks

Active Publication Date: 2019-03-19
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prevention strategy is mainly to cut off the transmission route, do a good job in chicken farm purification, strengthen sanitation and disinfection, and prevent hatching infection; enrofloxacin, ofloxacin, ciprofloxacin, neomycin, and chlorine are commonly used when hatching chicks start eating. Mixed drinking water or feed with various antimicrobial drugs such as amylcin, in view of the large amount and long-term use of antibiotics in chickens, accumulation, drug resistance and inconsistency between the results of in vitro susceptibility tests and in vivo conditions, it is always difficult to control Salmonella infection in chickens

Method used

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  • Fowl salmonella gallinarum attenuated isolated strain and application thereof
  • Fowl salmonella gallinarum attenuated isolated strain and application thereof
  • Fowl salmonella gallinarum attenuated isolated strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation, detection and identification of Salmonella strain SG01

[0023] The chicks (from the 2nd and 4th healthy flocks of the first poultry farm of the Mashan Poultry Group in Jiangsu Province, Zhao Jing (contact number 18852727750) collected 10 whole bodies of 360-day-old laying hens on October 18, 2016 Detect Salmonella in tissues and organs and keep samples in the laboratory, put them in an ultra-clean bench, disinfect the surface of the chicken body with alcohol, aseptically remove the liver, spleen, intestinal tract and other tissues of the chicken, place them in a sterile petri dish, and cut the tissues into pieces Grind at the same time, after weighing, draw the homogenate into a sterile test tube for use. Weigh buffered peptone water (BPW) and autoclave it for subpackage, then add the collected tissue samples to BPW for overnight cultivation at 37°C. Draw 1 mL of the culture solution and inoculate it in Carry out selective culture in selenite cyst...

Embodiment 2

[0035] Example 2: Research on the biological characteristics of Salmonella strain SG01

[0036] Inoculate a single colony of standard attenuated Salmonella typhi SG9R, isolate SG01 and virulent Salmonella typhi U20 in liquid LB at 37°C for overnight shaking culture, and absorb the bacterial liquid the next day and smear it on MacConkey, xylose-lysine The culture characteristics were compared in deoxycholate (XLD), milk of cholsulfide agar (DHL) plate and SC medium. Simultaneously compare the serotypes of the three strains; sucrose, lactose, glucose, raffinose, maltose, mannitol, indigo, mannose, citric acid, dulcitol, ornithine, lysine, potassium cyanide , hydrogen sulfide, urea, ONPG, MR test, V-P test, semi-solid agar, side marigold, nitrate reduction and other trace biochemical reactions and comparison of the growth curves of the three strains.

[0037] From the comparison of the culture characteristics of the three strains in Table 3, it was found that the isolate SG01 co...

Embodiment 3

[0049] Embodiment 3: Salmonella strain SG01 is to the infectivity test of 1-day-old chicks

[0050] 40 one-day-old SPF chicks were randomly divided into 5 groups, 8 in each group, and the isolated strains were divided into 1×10 7 cfu, 1×10 8 cfu, 1×10 9 cfu, 1×10 10 cfu inoculation, another PBS control group is established, the inoculation dose is 0.2mL / feather intramuscular injection, each group is isolated and fed, no antibiotic feed is fed, carefully observed for one week, and the diarrhea symptoms and other clinical symptoms (such as lameness) of all test chickens are observed every day. , eye secretions). The mortality rate and stress response were observed, and the organ lesions were observed by necropsy one week later, and the health status of the vaccinated chickens in each group was recorded.

[0051] The one-week test results found that the chickens in the isolate experimental group did not die, and the chickens were in good condition during the feeding process, ...

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Abstract

The invention relates to a fowl salmonella gallinarum attenuated isolated strain and application thereof. The isolated strain is preserved in the China General Microbiological Culture Collection Center, Beijing, China, on July 2, 2018, has a preservation number of CGMCC No.16049, and is classified and named as Salmonella Gallinarum. The strain is attenuated to chicken of different ages, is apathogenic, and can be used as a strain vector for preventing livestock salmonella gallinarum infection and preventing infectious diseases of livestock (chicken). The fowl salmonella gallinarum attenuated isolated strain provides data reference to evaluating the toxicity of salmonella attenuated vaccine strains and potential as the strain vector of livestock (chicken) live vaccines.

Description

field of invention [0001] The invention relates to the research on candidate strains of attenuated live vaccines for poultry bacteria infected by Salmonella typhi. The invention further provides a method for preventing typhoid salmonellosis in poultry, and is expected to provide candidate bacterial strain vectors for the development of effective live vaccines for preventing salmonella typhi. Background technique [0002] my country is a big poultry breeding country. In my country, there are various ways of poultry breeding, and intensive farming and courtyard farming coexist. In my country, the clinical isolation rate of Salmonella infection in chicken flocks is as high as 98.7%, which has to arouse people's great attention. The transmission mechanism of Salmonella in poultry is relatively complicated, and it can be transmitted horizontally, such as the contaminated environment, feed, drinking water and other channels of the digestive tract, eye conjunctiva and mating, and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K39/112A61P31/04C12R1/42
CPCA61K39/0275A61K2039/522A61K2039/542A61K2039/552A61P31/04C12N1/205C12R2001/42
Inventor 朱国强赵静魏星夏芃芃孟霞
Owner YANGZHOU UNIV
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