21 results about "Salmonella typhi" patented technology

The invention relates to a dual ERA-LFD kit for visually and rapidly detecting salmonella typhimurium or a single-phase variant thereof, a primer group and application of the dual ERA-LFD kit and the primer group, and aims at solving the problems that an existing detection method is complex in operation, long in time consumption and depends on professional equipment, and primer cross interference, high false positive rate and incapability of distinguishing the single-phase variant caused by the fact that dual ERA primer design is not standard. Screening to obtain a specific primer probe combination aiming at the fljB gene deletion region and the STM4495 gene; a dual ERA-LFD detection system is constructed, and double targets are synchronously amplified under a constant temperature condition; visual interpretation is realized through a double-detection-line color development mode of the transverse flow test strip. The detection specificity can be remarkably improved, and false positive is effectively avoided; the accurate distinguishing between the salmonella typhimurium and the single-phase variants is realized; the detection process is rapid and simple, does not need professional instruments and personnel, and is suitable for on-site rapid screening; the detection cost is obviously reduced and the basic application feasibility is improved.

The present application relates to the field of pharmaceutical chemistry, and specifically discloses a emodin mannich base, a preparation method and application thereof. The emodin mannich base has a structure as shown in formula I, the structure is simple, has strong in-vitro antimicrobial activity, especially high inhibitory activity to staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus vulgaris, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, monascus and other fungi, and can be used for preparing antibacterial and / or antifungal drugs.

The invention discloses a salmonella typhimurium aptamer lateral flow chromatography test strip based on the photothermal effect of a ferroferric oxide and colloidal gold composite nanomaterial, a preparation method and application, and belongs to the technical field of lateral flow chromatography test strips. The preparation of the probe comprises the step of preparing a Fe3O4 (at) Au composite nano material; the preparation method comprises the following steps: under the protection of nitrogen, dissolving FeCl3. 6H2O and FeCl2. 4H2O in deionized water, adding ammonia water, stirring at room temperature, adding trisodium citrate, collecting by using a magnet, then adding into a boiling HAuCl4 solution, keeping boiling until the color of the solution becomes reddish brown, continuing heating, cooling, and collecting by using a magnet; the test strip provided by the invention can realize quantitative detection of salmonella typhimurium.

The invention discloses a porcine rotavirus VP8 protein dominant B cell neutralizing epitope peptide, a multi-epitope fusion protein and application of the multi-epitope fusion protein. The FliCS.T-VP8 multi-epitope fusion protein prepared by the invention is a multi-epitope fusion protein which takes salmonella typhimurium flagellin FliCS.T as a molecular skeleton and integrates the dominant B cell neutralizing epitope of the VP8 protein of the porcine rotavirus. According to the design, immunodominance epitope screening, multi-epitope fusion protein construction and genetic engineering technologies are fused, and the immunogenicity and immune protection efficacy of the fusion protein are remarkably enhanced. The FliCS.T-VP8 multi-epitope fusion protein not only can effectively induce high-level VP8 protein specific antibody response and cellular immune response, but also shows good porcine rotavirus neutralizing activity. In addition, the fusion protein has relatively high safety and stability, and has the potential of being used as an efficient subunit vaccine for preventing and controlling porcine rotavirus infection in the pig industry.

The invention discloses application of lactobacillus plantarum Y40. The lactobacillus plantarum Y40 is used for improving the growth performance of meat ducks, improving the meat quality, enhancing the oxidation resistance and increasing the diversity of intestinal flora and the abundance of species. The duck meat color can be improved under the condition of salmonella typhimurium infection, the colonization level of pathogenic bacteria in immune organs of meat ducks is remarkably reduced, intestinal flora balance is remodeled, and the resistance of a host to salmonella typhimurium invasion is enhanced. The invention provides a new microbial resource for growth promotion, disease prevention and antibacterial application in livestock and poultry breeding, and has a good industrialization prospect.

The application discloses a 1, 2, 3-triazole sulfamide derivative, a preparation method and application thereof. The compound has a general formula (A). The synthesis method is as follows: sulfamide is used as a starting material, diazotization, azidation, and then coupling with ethyl acetoacetate to complete the docking of the triazole ring and the sulfamide, and an amide bond is introduced into the structure, and then a series of novel 1, 2, 3-triazole sulfamide derivatives are synthesized. The compounds have excellent bacteriostatic, insecticidal and fungicidal functions on escherichia coli, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus, bacillus subtilis, and fungi such as candida albicans and aspergillus niger, pests and plant pathogenic bacteria in forestry and other fields.

The invention is applicable to the technical field of microorganisms, and provides a lactobacillus paracasei Jlu-CH9 and application thereof, the lactobacillus paracasei Jlu-CH9 is preserved in China General Microbiological Culture Collection Center on January 4, 2026, the preservation number is CGMCC No.37281, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain Jlu-CH9 obtained by separation has certain tolerance to acid and cholate, has no hemolysis phenomenon, and is sensitive to various antibiotics; the compound has good inhibitory activity on dipeptidyl peptidase IV and has a certain potential of reducing blood sugar. The compound has relatively good inhibitory activity on a plurality of pathogenic bacteria including salmonella typhimurium, staphylococcus aureus, escherichia coli, clostridium perfringens and pseudomonas aeruginosa; the traditional Chinese medicine composition has good prevention and treatment effects on a DSS-induced mouse colitis model, and can repair intestinal barriers, regulate immune balance, remodel intestinal flora homeostasis and relieve colonic atrophy.

The invention belongs to the technical field of biology, and particularly relates to salmonella typhimurium flagellin capable of enhancing human TLR5 binding affinity and application of the salmonella typhimurium flagellin. A key amino acid mutation site of flagellin is screened and determined, and recombinant expression is realized in an escherichia coli system by adopting a site-directed mutagenesis technology. In-vitro experiments prove that the derivative can significantly activate the function of a TLR5 ligand. The invention solves the problem that the existing flagellin has a limited effect on activating the activity of the human TLR5, and provides a new technical scheme and theoretical basis for the application of the flagellin in the fields of vaccines, immunotherapeutic agents and radioprotectants.

The application provides a feed additive suitable for Danzhou chicken and a preparation method thereof, which comprises a Callicarpa nudiflora complex, a wine lees fermentation extract and an eleocharis tuberose peel; the wine lees fermentation extract is obtained by fermenting wheat flour and egg white powder with sweet wine lees, and then performing enzymatic dissolution with protease and glucanase, and then concentrating and drying; the feed additive suitable for Danzhou chicken is prepared from the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel; the feeding test proves that the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel have a good promoting effect on the production performance of Danzhou chicken, effectively improve the feed conversion rate, and reduce the mortality rate; and the inhibition rates of the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel on six kinds of bacteria, including Salmonella enteritidis, Salmonella typhi, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Proteus mirabilis, are all above 90%.

The invention belongs to the technical field of biology, and particularly relates to an establishment method and application of a pigeon salmonella acute infection model. The establishment method comprises the following steps: infecting a squab by adopting pigeon source salmonella typhimurium obtained by separation and screening in a subcervical multi-point injection manner, and preparing to obtain a pigeon salmonella acute infection model; the pigeon source salmonella typhimurium is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on August 25, 2025, and the preservation number of the pigeon source salmonella typhimurium is CGMCC N0.35198. According to the establishment method, the technical problems of low success rate and poor replication rate of an existing modeling method are solved, the pigeon salmonella acute infection model established by the method improves the modeling success rate and uniformity, is long in disease course and stable, can assist in research and development of drugs for preventing and treating pigeon salmonella infection, and can realize effective and objective evaluation of the drug effect.

The application discloses a kind of salmonella typhimurium aptamer lateral flow chromatography test strip based on ferroferric oxide@colloidal gold composite nanomaterial photo-thermal effect, preparation method and application, belong to lateral flow chromatography test strip technical field, the preparation method method includes preparation probe;The preparation probe includes preparation Fe3O4@Au composite nanomaterial;The preparation Fe3O4@Au composite nanomaterial, under nitrogen protection, FeCl3·6H2O and FeCl2·4H2O are dissolved in deionized water, add ammonia water, stirring at room temperature, add trisodium citrate, with magnet collection, then added to boiling HAuCl4 solution, keep boiling, until the solution color changes to red brown, continue heating, cooling, with magnet collection;The test strip of the application can realize the quantitative detection of salmonella typhimurium.

The present application relates to the technical field of microorganism, and especially relates to a kind of Enterococcus durans C24, probiotic agent and application thereof.The present application provides Enterococcus durans C24, and its preservation number is CGMCC No.33995.The strain has broad-spectrum antibacterial activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, and has good stress resistance, can survive in gastrointestinal environment, and also has high biological safety, and is expected to be used for clinical prevention and control of salmonellosis.

The application discloses a Salmonella multi-epitope protein, a coding gene and application of the Salmonella multi-epitope protein in preparation of a Salmonella subunit vaccine, and relates to the field of biotechnology.The amino acid sequence of the Salmonella multi-epitope protein is shown as SEQ ID NO.2.The nucleotide sequence of the coding gene of the Salmonella multi-epitope protein is shown as SEQ ID NO.1.The Salmonella subunit vaccine prepared by using the Salmonella multi-epitope protein has the advantages of safety and stability, low cost, no toxicity to the body, and the ability to simultaneously cause the body to produce humoral immunity and cellular immunity, and the like, and can not only produce a preventive protection effect on Salmonella enteritidis infection, but also can play a cross protection effect on Salmonella typhimurium infection.

The application discloses Parabacteroides distasonis capable of inhibiting pathogenic bacteria and resisting tumors and an application thereof, relates to the technical field of microorganisms, and has the following technical points: the preservation number of Parabacteroides distasonis NGMCC 1.200926 is CGMCC No: 40687; the preservation address is No. 3, Xili Beichen, Chaoyang District, Beijing; the preservation unit is the General Microbiological Center of China Microorganism Culture Collection Committee; and the preservation time is July 5, 2023. After fermentation culture of Parabacteroides distasonis NGMCC 1.2000926, strong bacteriostatic active substances can be generated, the pathogenic bacteria such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa have obvious bacteriostatic effects, and the bacteriostatic active substances have good tumor cell activity, so that the application has a broad development space in the prevention and treatment of diseases caused by the pathogenic bacteria such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa and tumors, and has a good development and application prospect in antibacterial drugs and antitumor drugs.

The invention discloses a lactoferrin modified florfenicol nano-liposome (R-FL) as well as a preparation method and application thereof. The R-FL is prepared by adopting a technology of combining an improved reversed-phase solvent evaporation method and chemical coupling. The R-FL disclosed by the invention is strong in slow and controlled release capability and good in biocompatibility, has excellent in-vitro and in-vivo antibacterial effects, and can be used for inhibiting the formation of a biofilm by precisely damaging the structure of the biofilm of the drug-resistant salmonella typhimurium in a targeting manner. The R-FL can effectively regulate the intestinal flora disorder caused by the drug-resistant salmonella typhimurium, so that the intestinal flora is recovered to a normal level. The R-FL can be used as a novel nano preparation with great potential for resisting drug-resistant salmonella typhimurium disease, and has a wide application prospect.

The present invention discloses an anti-Salmonella typhimurium antibody, immunomagnetic beads labeled therewith, and applications thereof. The present invention provides an anti-Salmonella typhimurium monoclonal antibody comprising a light chain variable region and a heavy chain variable region, wherein LCDR1 of the light chain variable region comprises the amino acid sequence shown in SEQ ID NO:1, LCDR2 comprises the amino acid sequence LAS, and LCDR3 comprises the sequence shown in SEQ ID NO:2; and HCDR1 to HCDR3 of the heavy chain variable region comprise the sequences shown in SEQ ID NO:3, 4, and 5, respectively. The present invention establishes a Salmonella typhimurium immunomagnetic bead enrichment and separation method based on the specific monoclonal antibody against Salmonella typhimurium. The method is rapid and simple, has a high capture rate, strong specificity, and good sensitivity.

This invention discloses an antimicrobial peptide, sesquimycin, and its applications. Specifically, it involves eight sesquimycin-like polypeptide compounds. Compounds 1-8 exhibit varying degrees of antimicrobial activity, with compound 2 possessing broad-spectrum antimicrobial activity. Its minimum inhibitory concentration (MIC) against *Staphylococcus aureus* ATCC 6538 and *Bacillus cereus* CMCC(B)63301 reaches 20 μg / mL, and its MIC against *Enterococcus faecalis* 160119481, *Salmonella typhi* CMCC(B)50071, and *Salmonella typhimurium* CMCC(B)50115 is 40 μg / mL. Given that *Staphylococcus*, *Bacillus*, *Enterococcus*, and *Salmonella* are common foodborne pathogens posing a serious threat to food safety, compounds 1-8 can be used to prepare novel antimicrobial peptides for application in the field of food safety preservation.

The invention relates to the technical field of pullorum disease, in particular to a PCR (Polymerase Chain Reaction) detection kit for quickly identifying salmonella gallinarum with pullorum disease, which comprises a detection system and a shell, and a top cover is hinged to one side of the top of the shell; a loading plate is vertically arranged in the shell in a sliding fit manner, a plurality of loading ports for loading PCR tubes are formed in the loading plate, and loading bags are fixedly connected to the loading ports; an oscillating assembly used for oscillating the loading bag and a limiting assembly used for limiting the position of the loading plate are arranged in the shell. The oscillation assembly comprises an oscillation plate, a controller and a driving part, and the controller is used for controlling the driving part to operate; the oscillating plate is located below the loading bag and is in vertical sliding fit with the inner side wall of the shell; the driving piece is fixedly connected to the inner bottom wall of the shell, and an output shaft of the driving piece is fixedly connected with a stirring wheel. Through the synergistic effect, the oscillation plate can achieve the efficient oscillation effect, it is ensured that reactants are evenly distributed in a PCR tube, and the uniformity and efficiency of the PCR reaction are improved.

This invention proposes a droplet digital PCR method for simultaneously detecting the quantity of four foodborne pathogens. The steps include: 1. Preparing the DNA sample to be tested; 2. Preparing primers and probes for the four bacteria to be tested; the four bacteria to be tested are Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus; 3. Using a ddPCR amplification reaction system, which includes the primers and probes for the four bacteria to be tested in step 2, amplifying the DNA sample to be tested in step 1, detecting the amplification products, and obtaining the quantitative results of the four bacteria. This invention can simultaneously detect four pathogenic microorganisms: Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus. It establishes a precise, rapid, and absolute quantitative detection system for the simultaneous detection of these four pathogens using multiplex ddPCR technology, exhibiting high specificity, high precision, and strong repeatability.

The invention provides lactobacillus reuteri MRL1103 and application thereof, and relates to the technical field of microorganisms and mercury adsorption, the preservation number of the lactobacillus reuteri MRL1103 with a mercury adsorption function is CGMCC NO.28840, the lactobacillus reuteri MRL1103 is separated from fresh chicken manure, the lactobacillus reuteri MRL1103 can tolerate a mercury solution with the concentration as high as 100 mg / L, and under the conditions that the pH is 5 and the concentration of the mercury solution is 5 mg / L, the concentration of the mercury solution can reach 100 mg / L, and the concentration of the mercury solution can reach 100 mg / L; the adsorption rate of the lactobacillus reuteri MRL1103 on mercury in a mercury solution reaches 95% or above, and the lactobacillus reuteri MRL1103 has an inhibition effect on escherichia coli, staphylococcus aureus, salmonella enteritidis, salmonella pullorum or salmonella typhimurium. The lactobacillus reuteri MRL1103 can be used for removing a mercury metal element in a matrix, and the matrix can be mercury-containing soil, a feed or a culture medium.

The application discloses a sulfonamide derivative containing pyrazole and 1,2,3-triazole, a synthetic method and application. The compound has structures as shown in formula I and formula II. The synthetic method is as follows: taking sulfonamide as a starting material, performing diazotization, azidation, coupling with acetylacetone and ethyl acetoacetate to complete the sulfonamide containing pyrazole and triazole ring, introducing an amide bond in the structure, and then synthesizing a series of novel sulfonamide derivatives containing pyrazole and triazole. The preparation method is simple, and the prepared compound shows excellent bacteriostatic, insecticidal and fungicidal functions on escherichia coli, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus, bacillus subtilis, and other bacteria, such as candida albicans and aspergillus niger, pests and plant pathogenic fungi in forestry and other fields.