13 results about "Salmonella typhi" patented technology

The invention discloses application of lactobacillus plantarum Y40. The lactobacillus plantarum Y40 is used for improving the growth performance of meat ducks, improving the meat quality, enhancing the oxidation resistance and increasing the diversity of intestinal flora and the abundance of species. The duck meat color can be improved under the condition of salmonella typhimurium infection, the colonization level of pathogenic bacteria in immune organs of meat ducks is remarkably reduced, intestinal flora balance is remodeled, and the resistance of a host to salmonella typhimurium invasion is enhanced. The invention provides a new microbial resource for growth promotion, disease prevention and antibacterial application in livestock and poultry breeding, and has a good industrialization prospect.

The application discloses a 1, 2, 3-triazole sulfamide derivative, a preparation method and application thereof. The compound has a general formula (A). The synthesis method is as follows: sulfamide is used as a starting material, diazotization, azidation, and then coupling with ethyl acetoacetate to complete the docking of the triazole ring and the sulfamide, and an amide bond is introduced into the structure, and then a series of novel 1, 2, 3-triazole sulfamide derivatives are synthesized. The compounds have excellent bacteriostatic, insecticidal and fungicidal functions on escherichia coli, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus, bacillus subtilis, and fungi such as candida albicans and aspergillus niger, pests and plant pathogenic bacteria in forestry and other fields.

The invention is applicable to the technical field of microorganisms, and provides a lactobacillus paracasei Jlu-CH9 and application thereof, the lactobacillus paracasei Jlu-CH9 is preserved in China General Microbiological Culture Collection Center on January 4, 2026, the preservation number is CGMCC No.37281, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain Jlu-CH9 obtained by separation has certain tolerance to acid and cholate, has no hemolysis phenomenon, and is sensitive to various antibiotics; the compound has good inhibitory activity on dipeptidyl peptidase IV and has a certain potential of reducing blood sugar. The compound has relatively good inhibitory activity on a plurality of pathogenic bacteria including salmonella typhimurium, staphylococcus aureus, escherichia coli, clostridium perfringens and pseudomonas aeruginosa; the traditional Chinese medicine composition has good prevention and treatment effects on a DSS-induced mouse colitis model, and can repair intestinal barriers, regulate immune balance, remodel intestinal flora homeostasis and relieve colonic atrophy.

The invention belongs to the technical field of biology, and particularly relates to salmonella typhimurium flagellin capable of enhancing human TLR5 binding affinity and application of the salmonella typhimurium flagellin. A key amino acid mutation site of flagellin is screened and determined, and recombinant expression is realized in an escherichia coli system by adopting a site-directed mutagenesis technology. In-vitro experiments prove that the derivative can significantly activate the function of a TLR5 ligand. The invention solves the problem that the existing flagellin has a limited effect on activating the activity of the human TLR5, and provides a new technical scheme and theoretical basis for the application of the flagellin in the fields of vaccines, immunotherapeutic agents and radioprotectants.

The invention belongs to the technical field of biology, and particularly relates to an establishment method and application of a pigeon salmonella acute infection model. The establishment method comprises the following steps: infecting a squab by adopting pigeon source salmonella typhimurium obtained by separation and screening in a subcervical multi-point injection manner, and preparing to obtain a pigeon salmonella acute infection model; the pigeon source salmonella typhimurium is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on August 25, 2025, and the preservation number of the pigeon source salmonella typhimurium is CGMCC N0.35198. According to the establishment method, the technical problems of low success rate and poor replication rate of an existing modeling method are solved, the pigeon salmonella acute infection model established by the method improves the modeling success rate and uniformity, is long in disease course and stable, can assist in research and development of drugs for preventing and treating pigeon salmonella infection, and can realize effective and objective evaluation of the drug effect.

The application discloses a kind of salmonella typhimurium aptamer lateral flow chromatography test strip based on ferroferric oxide@colloidal gold composite nanomaterial photo-thermal effect, preparation method and application, belong to lateral flow chromatography test strip technical field, the preparation method method includes preparation probe;The preparation probe includes preparation Fe3O4@Au composite nanomaterial;The preparation Fe3O4@Au composite nanomaterial, under nitrogen protection, FeCl3·6H2O and FeCl2·4H2O are dissolved in deionized water, add ammonia water, stirring at room temperature, add trisodium citrate, with magnet collection, then added to boiling HAuCl4 solution, keep boiling, until the solution color changes to red brown, continue heating, cooling, with magnet collection;The test strip of the application can realize the quantitative detection of salmonella typhimurium.

The present application relates to the technical field of microorganism, and especially relates to a kind of Enterococcus durans C24, probiotic agent and application thereof.The present application provides Enterococcus durans C24, and its preservation number is CGMCC No.33995.The strain has broad-spectrum antibacterial activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, and has good stress resistance, can survive in gastrointestinal environment, and also has high biological safety, and is expected to be used for clinical prevention and control of salmonellosis.

The application discloses a Salmonella multi-epitope protein, a coding gene and application of the Salmonella multi-epitope protein in preparation of a Salmonella subunit vaccine, and relates to the field of biotechnology.The amino acid sequence of the Salmonella multi-epitope protein is shown as SEQ ID NO.2.The nucleotide sequence of the coding gene of the Salmonella multi-epitope protein is shown as SEQ ID NO.1.The Salmonella subunit vaccine prepared by using the Salmonella multi-epitope protein has the advantages of safety and stability, low cost, no toxicity to the body, and the ability to simultaneously cause the body to produce humoral immunity and cellular immunity, and the like, and can not only produce a preventive protection effect on Salmonella enteritidis infection, but also can play a cross protection effect on Salmonella typhimurium infection.

The application discloses Parabacteroides distasonis capable of inhibiting pathogenic bacteria and resisting tumors and an application thereof, relates to the technical field of microorganisms, and has the following technical points: the preservation number of Parabacteroides distasonis NGMCC 1.200926 is CGMCC No: 40687; the preservation address is No. 3, Xili Beichen, Chaoyang District, Beijing; the preservation unit is the General Microbiological Center of China Microorganism Culture Collection Committee; and the preservation time is July 5, 2023. After fermentation culture of Parabacteroides distasonis NGMCC 1.2000926, strong bacteriostatic active substances can be generated, the pathogenic bacteria such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa have obvious bacteriostatic effects, and the bacteriostatic active substances have good tumor cell activity, so that the application has a broad development space in the prevention and treatment of diseases caused by the pathogenic bacteria such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa and tumors, and has a good development and application prospect in antibacterial drugs and antitumor drugs.

This invention discloses an antimicrobial peptide, sesquimycin, and its applications. Specifically, it involves eight sesquimycin-like polypeptide compounds. Compounds 1-8 exhibit varying degrees of antimicrobial activity, with compound 2 possessing broad-spectrum antimicrobial activity. Its minimum inhibitory concentration (MIC) against *Staphylococcus aureus* ATCC 6538 and *Bacillus cereus* CMCC(B)63301 reaches 20 μg / mL, and its MIC against *Enterococcus faecalis* 160119481, *Salmonella typhi* CMCC(B)50071, and *Salmonella typhimurium* CMCC(B)50115 is 40 μg / mL. Given that *Staphylococcus*, *Bacillus*, *Enterococcus*, and *Salmonella* are common foodborne pathogens posing a serious threat to food safety, compounds 1-8 can be used to prepare novel antimicrobial peptides for application in the field of food safety preservation.

This invention proposes a droplet digital PCR method for simultaneously detecting the quantity of four foodborne pathogens. The steps include: 1. Preparing the DNA sample to be tested; 2. Preparing primers and probes for the four bacteria to be tested; the four bacteria to be tested are Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus; 3. Using a ddPCR amplification reaction system, which includes the primers and probes for the four bacteria to be tested in step 2, amplifying the DNA sample to be tested in step 1, detecting the amplification products, and obtaining the quantitative results of the four bacteria. This invention can simultaneously detect four pathogenic microorganisms: Staphylococcus aureus, Listeria monocytogenes, Salmonella typhi, and Bacillus cereus. It establishes a precise, rapid, and absolute quantitative detection system for the simultaneous detection of these four pathogens using multiplex ddPCR technology, exhibiting high specificity, high precision, and strong repeatability.

The invention provides lactobacillus reuteri MRL1103 and application thereof, and relates to the technical field of microorganisms and mercury adsorption, the preservation number of the lactobacillus reuteri MRL1103 with a mercury adsorption function is CGMCC NO.28840, the lactobacillus reuteri MRL1103 is separated from fresh chicken manure, the lactobacillus reuteri MRL1103 can tolerate a mercury solution with the concentration as high as 100 mg / L, and under the conditions that the pH is 5 and the concentration of the mercury solution is 5 mg / L, the concentration of the mercury solution can reach 100 mg / L, and the concentration of the mercury solution can reach 100 mg / L; the adsorption rate of the lactobacillus reuteri MRL1103 on mercury in a mercury solution reaches 95% or above, and the lactobacillus reuteri MRL1103 has an inhibition effect on escherichia coli, staphylococcus aureus, salmonella enteritidis, salmonella pullorum or salmonella typhimurium. The lactobacillus reuteri MRL1103 can be used for removing a mercury metal element in a matrix, and the matrix can be mercury-containing soil, a feed or a culture medium.

The application discloses a sulfonamide derivative containing pyrazole and 1,2,3-triazole, a synthetic method and application. The compound has structures as shown in formula I and formula II. The synthetic method is as follows: taking sulfonamide as a starting material, performing diazotization, azidation, coupling with acetylacetone and ethyl acetoacetate to complete the sulfonamide containing pyrazole and triazole ring, introducing an amide bond in the structure, and then synthesizing a series of novel sulfonamide derivatives containing pyrazole and triazole. The preparation method is simple, and the prepared compound shows excellent bacteriostatic, insecticidal and fungicidal functions on escherichia coli, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus, bacillus subtilis, and other bacteria, such as candida albicans and aspergillus niger, pests and plant pathogenic fungi in forestry and other fields.