The invention discloses a Chinese-Western combination sterilizing and disinfecting solution and a preparation method thereof, which belong to the technical field of medicine; the ingredients of the sterilizing and disinfecting solution of the invention are composed of the following raw materials: Puhuang, Campanula, Spirulina, Artemisia arvensis, Peach Blossom and Yahuazi , Jianweifeng, Lotus Pod, Saijingang, Marigold, Eucalyptus blue, Cinnabar Root, Catalpa Bark, Duckweed, Norfloxacin, Cresyl Sulfonic Acid and Triclosan; The treatment method is better than the simple use of traditional Chinese medicine and western medicine. It has the effects of killing insects and disinfecting, clearing away heat and detoxifying, antibacterial and anti-inflammatory, and can maximize the efficacy of Chinese medicine ingredients and western medicine. , has a good inhibitory effect on Staphylococcus aureus, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Proteus vulgaris, etc., with remarkable effect and low cost.