38 results about "Salmonella typhi" patented technology

The invention discloses a chicken infectious anemia virus subunit vaccine, and belongs to the technical field of animal disease vaccines. The invention provides a chicken infectious anemia virus subunit vaccine. Active ingredients of the chicken infectious anemia virus subunit vaccine are recombinant fusion protein of chicken infectious anemia virus VP1 protein B cell epitope and salmonella typhimurium flagellin. The B cell epitope of the chicken infectious anemia virus VP1 protein is obtained by connecting B cell antigen epitopes contained in the chicken infectious anemia virus VP1 protein through a connecting peptide. The salmonella typhimurium flagellin is obtained by deleting a hypervariable region of the salmonella typhimurium flagellin FliC. The fusion expression protein provided by the invention can induce generation of a specific IgG antibody after one-day-old SPF chicken, and after immunization for 28 days, immunized chicken flocks can resist challenge of chicken infectious anemia virus virulent strains, and 70% of challenge chicken flocks are protected without disease attack. The invention has great potential for clinical prevention and control of chicken infectious anemia virus infection.

The present invention belongs to the field of biotechnology and relates to a five - plex fluorescence PCR detection reagent for Salmonella, Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum and Salmonella gallinarum and its application. By using the five - plex fluorescence PCR detection reagent of the present invention, the purpose of simultaneously identifying and detecting Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum and Salmonella gallinarum can be achieved. It has the advantages of simple operation, rapidity, high accuracy, high sensitivity and good repeatability, and is of great significance for the rapid and accurate detection of clinical animal salmonellosis and epidemic disease prevention and control.

The invention discloses a preparation method of a water-soluble CsPbBr3 perovskite quantum dot fluorescence sensing array and application of the water-soluble CsPbBr3 perovskite quantum dot fluorescence sensing array in rapid detection and inactivation of food-borne pathogenic bacteria. The sensor array is composed of three perovskite quantum dots (respectively named as PQD1, PQD2 and PQD3) with different fluorescence characteristics, wherein the perovskite quantum dots are synthesized at room temperature. The feasibility of constructing the sensing platform is verified through different fluorescence responses after the sensing platform is combined with five food-borne pathogenic bacteria (escherichia coli, staphylococcus aureus, salmonella typhimurium, listeria monocytogenes and pseudomonas aeruginosa). The relative signal intensity change is further analyzed through a machine learning algorithm, and rapid recognition and identification of pathogenic bacteria are achieved. In addition, the sensing array shows high practicability and accuracy in actual sample detection, and pathogenic bacteria can be effectively inactivated after 30 minutes of detection. The invention provides a novel efficient and reliable technical solution for food safety detection.

The invention relates to a dual ERA-LFD kit for visually and rapidly detecting salmonella typhimurium or a single-phase variant thereof, a primer group and application of the dual ERA-LFD kit and the primer group, and aims at solving the problems that an existing detection method is complex in operation, long in time consumption and depends on professional equipment, and primer cross interference, high false positive rate and incapability of distinguishing the single-phase variant caused by the fact that dual ERA primer design is not standard. Screening to obtain a specific primer probe combination aiming at the fljB gene deletion region and the STM4495 gene; a dual ERA-LFD detection system is constructed, and double targets are synchronously amplified under a constant temperature condition; visual interpretation is realized through a double-detection-line color development mode of the transverse flow test strip. The detection specificity can be remarkably improved, and false positive is effectively avoided; the accurate distinguishing between the salmonella typhimurium and the single-phase variants is realized; the detection process is rapid and simple, does not need professional instruments and personnel, and is suitable for on-site rapid screening; the detection cost is obviously reduced and the basic application feasibility is improved.

The invention belongs to the technical field of veterinary drugs, and discloses a Chinese pulsatilla root alcohol extract and gentamicin composition and application thereof in inhibition of drug-resistant salmonella typhimurium. The pulsatilla alcohol extract composition provided by the invention comprises pulsatilla alcohol extract and gentamicin, and for salmonella typhimurium, the optimal ratio concentration of the pulsatilla alcohol extract to the gentamicin is 31.25 mg / mL: 2 [mu] g / ml; the Chinese pulsatilla root alcohol extract and the gentamicin have the synergistic bacteriostatic effect on salmonella typhimurium, the composition reduces the concentration of single bacteriostasis of the Chinese pulsatilla root alcohol extract and the gentamicin, the cost is saved, and the composition has wide application prospects and development value in the fields of food processing, medicine and breeding industry.

The present application relates to the field of pharmaceutical chemistry, and specifically discloses a emodin mannich base, a preparation method and application thereof. The emodin mannich base has a structure as shown in formula I, the structure is simple, has strong in-vitro antimicrobial activity, especially high inhibitory activity to staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteus vulgaris, pseudomonas aeruginosa, shigella dysenteriae, salmonella typhimurium and other bacteria, and candida utilis, candida albicans, saccharomyces cerevisiae, monascus and other fungi, and can be used for preparing antibacterial and / or antifungal drugs.

The present invention discloses a subunit vaccine against chicken infectious anemia virus, belonging to the technical field of animal disease vaccines. The present invention provides a subunit vaccine against chicken infectious anemia virus, and the active ingredient is a recombinant fusion protein of the B cell epitope of chicken infectious anemia virus VP1 protein and Salmonella typhimurium flagellin. The B cell epitope of chicken infectious anemia virus VP1 protein is obtained by connecting the B cell antigen epitopes contained in chicken infectious anemia virus VP1 protein through a linker peptide. The Salmonella typhimurium flagellin is obtained by deleting the hypervariable region of Salmonella typhimurium flagellin FliC. After the fusion expression protein of the present invention is administered to 1-day-old SPF chickens, it can induce the production of specific IgG antibodies, and after 28 days of immunization, the immunized chicken flock can resist the challenge of the virulent strain of chicken infectious anemia virus, and 70% of the challenged chicken flock is protected from disease. The present invention has great potential for clinical prevention and control of chicken infectious anemia virus infection.

The invention discloses a salmonella typhimurium aptamer lateral flow chromatography test strip based on the photothermal effect of a ferroferric oxide and colloidal gold composite nanomaterial, a preparation method and application, and belongs to the technical field of lateral flow chromatography test strips. The preparation of the probe comprises the step of preparing a Fe3O4 (at) Au composite nano material; the preparation method comprises the following steps: under the protection of nitrogen, dissolving FeCl3. 6H2O and FeCl2. 4H2O in deionized water, adding ammonia water, stirring at room temperature, adding trisodium citrate, collecting by using a magnet, then adding into a boiling HAuCl4 solution, keeping boiling until the color of the solution becomes reddish brown, continuing heating, cooling, and collecting by using a magnet; the test strip provided by the invention can realize quantitative detection of salmonella typhimurium.

The present invention discloses a tumor-targeting typhoid toxin B subunit mimetic peptide and its application. The typhoid toxin B subunit mimetic peptide is selected from any one of the six polypeptides YQPB-1: COOH-Thr-Gly-Ser-Gly-Asn-Ala-Asn-Val-NH2. The application of the typhoid toxin B subunit mimetic peptide or its dimer and multimer described in the present invention in the preparation of diagnostic reagents or tumor therapeutic drugs targeting tumors. The present invention has developed a series of new mimetic peptides that simulate the typhoid toxin B subunit (PltB) secreted by Salmonella typhi. The YQPB-X series of mimetic peptides can specifically bind to tumor cells with high expression of gangliosides. In vivo optical imaging and results have confirmed that they have excellent imaging results for various tumors such as neuroblastoma, non-small cell lung cancer, melanoma, breast cancer and osteosarcoma.

The invention discloses a porcine rotavirus VP8 protein dominant B cell neutralizing epitope peptide, a multi-epitope fusion protein and application of the multi-epitope fusion protein. The FliCS.T-VP8 multi-epitope fusion protein prepared by the invention is a multi-epitope fusion protein which takes salmonella typhimurium flagellin FliCS.T as a molecular skeleton and integrates the dominant B cell neutralizing epitope of the VP8 protein of the porcine rotavirus. According to the design, immunodominance epitope screening, multi-epitope fusion protein construction and genetic engineering technologies are fused, and the immunogenicity and immune protection efficacy of the fusion protein are remarkably enhanced. The FliCS.T-VP8 multi-epitope fusion protein not only can effectively induce high-level VP8 protein specific antibody response and cellular immune response, but also shows good porcine rotavirus neutralizing activity. In addition, the fusion protein has relatively high safety and stability, and has the potential of being used as an efficient subunit vaccine for preventing and controlling porcine rotavirus infection in the pig industry.

The invention discloses application of lactobacillus plantarum Y40. The lactobacillus plantarum Y40 is used for improving the growth performance of meat ducks, improving the meat quality, enhancing the oxidation resistance and increasing the diversity of intestinal flora and the abundance of species. The duck meat color can be improved under the condition of salmonella typhimurium infection, the colonization level of pathogenic bacteria in immune organs of meat ducks is remarkably reduced, intestinal flora balance is remodeled, and the resistance of a host to salmonella typhimurium invasion is enhanced. The invention provides a new microbial resource for growth promotion, disease prevention and antibacterial application in livestock and poultry breeding, and has a good industrialization prospect.

The invention discloses a nucleic acid detection kit for typhoid fever, paratyphoid fever, cholera, shigella and dysentery. The nucleic acid detection kit comprises a multiple fluorescent PCR detection system, and the multiple fluorescent PCR detection system comprises a typhoid fever salmonella primer probe, a paratyphoid fever salmonella primer probe, a vibrio cholera primer probe, a dysentery amoeba primer probe and a shigella primer probe; according to the kit, specific and conservative characteristic genes of different bacteria species are selected for primer probe design, so that the specificity, the accuracy and the sensitivity of detection are ensured.

The application discloses a 1, 2, 3-triazole sulfamide derivative, a preparation method and application thereof. The compound has a general formula (A). The synthesis method is as follows: sulfamide is used as a starting material, diazotization, azidation, and then coupling with ethyl acetoacetate to complete the docking of the triazole ring and the sulfamide, and an amide bond is introduced into the structure, and then a series of novel 1, 2, 3-triazole sulfamide derivatives are synthesized. The compounds have excellent bacteriostatic, insecticidal and fungicidal functions on escherichia coli, pseudomonas aeruginosa, salmonella typhi, staphylococcus aureus, bacillus subtilis, and fungi such as candida albicans and aspergillus niger, pests and plant pathogenic bacteria in forestry and other fields.

The invention discloses a specific antigen fragment of salmonella typhimurium and application of the specific antigen fragment in detection of a salmonella typhimurium antibody. The amino acid sequence of the salmonella typhimurium specific antigen fragment is as shown in SEQ ID NO. 1. The invention discloses a method for detecting the indirect ELISA antibody of the salmonella typhimurium by taking the specific antigen fragment of the salmonella typhimurium as the coating antigen of the indirect ELISA for the first time, the specific antigen fragment of the salmonella typhimurium contains dominant B cell epitopes of the FliC protein of the salmonella typhimurium, and the specific antigen fragment of the salmonella typhimurium contains the dominant B cell epitopes of the FliC protein of the salmonella typhimurium. The salmonella typhimurium FliC protein antibody generated by induction can be specifically recognized, and the obvious synergistic effect is achieved in the aspect of improving the specific binding of the antigen and the antibody. The kit can be used for detecting the salmonella typhimurium antibody, and provides powerful technical support for epidemiological investigation of salmonella typhimurium and prevention and control of salmonella.

The invention is applicable to the technical field of microorganisms, and provides a lactobacillus paracasei Jlu-CH9 and application thereof, the lactobacillus paracasei Jlu-CH9 is preserved in China General Microbiological Culture Collection Center on January 4, 2026, the preservation number is CGMCC No.37281, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain Jlu-CH9 obtained by separation has certain tolerance to acid and cholate, has no hemolysis phenomenon, and is sensitive to various antibiotics; the compound has good inhibitory activity on dipeptidyl peptidase IV and has a certain potential of reducing blood sugar. The compound has relatively good inhibitory activity on a plurality of pathogenic bacteria including salmonella typhimurium, staphylococcus aureus, escherichia coli, clostridium perfringens and pseudomonas aeruginosa; the traditional Chinese medicine composition has good prevention and treatment effects on a DSS-induced mouse colitis model, and can repair intestinal barriers, regulate immune balance, remodel intestinal flora homeostasis and relieve colonic atrophy.

The invention belongs to the technical field of biology, and particularly relates to salmonella typhimurium flagellin capable of enhancing human TLR5 binding affinity and application of the salmonella typhimurium flagellin. A key amino acid mutation site of flagellin is screened and determined, and recombinant expression is realized in an escherichia coli system by adopting a site-directed mutagenesis technology. In-vitro experiments prove that the derivative can significantly activate the function of a TLR5 ligand. The invention solves the problem that the existing flagellin has a limited effect on activating the activity of the human TLR5, and provides a new technical scheme and theoretical basis for the application of the flagellin in the fields of vaccines, immunotherapeutic agents and radioprotectants.

The invention provides a salmonella typhimurium ArcB gene deleted strain as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The construction method comprises the following steps: amplifying target DNA (deoxyribonucleic acid); the pKD46 plasmid is electrically transformed into an SL1344 competent cell, and then the targeting DNA is electrically transformed into the SL1344 containing the pKD46 plasmid, so that a deleted strain containing the Kan resistance gene is obtained; after the pCP20 plasmid is electrically transferred into the deleted strain containing the kan resistance gene, the pCP20 plasmid is eliminated; and after the sequencing is correct, obtaining the ArcB gene deleted strain SL1344 [delta] ArcB. The acid resistance and hypertonic resistance of the SL1344deltaArcB provided by the invention are remarkably weakened compared with those of WT, the sensitivity to penicillin, imipenem, kanamycin and amikacin is remarkably improved, the sensitivity to gentamicin is remarkably reduced, and the SL1344deltaArcB has important significance in research on the aspects of drug resistance, toxicity, vaccines and the like of salmonella typhimurium.

The application provides a feed additive suitable for Danzhou chicken and a preparation method thereof, which comprises a Callicarpa nudiflora complex, a wine lees fermentation extract and an eleocharis tuberose peel; the wine lees fermentation extract is obtained by fermenting wheat flour and egg white powder with sweet wine lees, and then performing enzymatic dissolution with protease and glucanase, and then concentrating and drying; the feed additive suitable for Danzhou chicken is prepared from the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel; the feeding test proves that the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel have a good promoting effect on the production performance of Danzhou chicken, effectively improve the feed conversion rate, and reduce the mortality rate; and the inhibition rates of the Callicarpa nudiflora complex, the wine lees fermentation extract and the eleocharis tuberose peel on six kinds of bacteria, including Salmonella enteritidis, Salmonella typhi, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Proteus mirabilis, are all above 90%.

The invention belongs to the technical field of biology, and particularly relates to an establishment method and application of a pigeon salmonella acute infection model. The establishment method comprises the following steps: infecting a squab by adopting pigeon source salmonella typhimurium obtained by separation and screening in a subcervical multi-point injection manner, and preparing to obtain a pigeon salmonella acute infection model; the pigeon source salmonella typhimurium is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on August 25, 2025, and the preservation number of the pigeon source salmonella typhimurium is CGMCC N0.35198. According to the establishment method, the technical problems of low success rate and poor replication rate of an existing modeling method are solved, the pigeon salmonella acute infection model established by the method improves the modeling success rate and uniformity, is long in disease course and stable, can assist in research and development of drugs for preventing and treating pigeon salmonella infection, and can realize effective and objective evaluation of the drug effect.

The application discloses a kind of salmonella typhimurium aptamer lateral flow chromatography test strip based on ferroferric oxide@colloidal gold composite nanomaterial photo-thermal effect, preparation method and application, belong to lateral flow chromatography test strip technical field, the preparation method method includes preparation probe;The preparation probe includes preparation Fe3O4@Au composite nanomaterial;The preparation Fe3O4@Au composite nanomaterial, under nitrogen protection, FeCl3·6H2O and FeCl2·4H2O are dissolved in deionized water, add ammonia water, stirring at room temperature, add trisodium citrate, with magnet collection, then added to boiling HAuCl4 solution, keep boiling, until the solution color changes to red brown, continue heating, cooling, with magnet collection;The test strip of the application can realize the quantitative detection of salmonella typhimurium.

The present invention discloses a rapid identification kit for Salmonella pullorum and Salmonella typhi based on res gene. The kit comprises detection primers, positive control, negative control and blank control, the positive control is Salmonella pullorum ATCC9184 genomic DNA, the negative control is Salmonella pullorum ATCC10398 genomic DNA, the blank control is sterilized double distilled water, and the primers comprise: For: 5'-ATCTCATCAACCAGCATACT-3'; Rev: 5'-TTCTTACGCAGCAATGAG-3'. The present invention also discloses a PCR method for rapid identification of two biotypes of Salmonella pullorum and Salmonella typhi serotypes in Salmonella typhi serotypes using res gene as detection target. The method of the present invention has the advantages of being fast, simple, highly specific and highly sensitive. Compared with the traditional biochemical identification method of Salmonella pullorum and Salmonella typhi, it has great advantages in detection time and detection cost, and is suitable for clinical batch detection.

The present application relates to the technical field of microorganism, and especially relates to a kind of Enterococcus durans C24, probiotic agent and application thereof.The present application provides Enterococcus durans C24, and its preservation number is CGMCC No.33995.The strain has broad-spectrum antibacterial activity, can inhibit Salmonella enteritidis, Salmonella pullorum and Salmonella typhimurium, has no hemolytic activity, and has good stress resistance, can survive in gastrointestinal environment, and also has high biological safety, and is expected to be used for clinical prevention and control of salmonellosis.

The application discloses a Salmonella multi-epitope protein, a coding gene and application of the Salmonella multi-epitope protein in preparation of a Salmonella subunit vaccine, and relates to the field of biotechnology.The amino acid sequence of the Salmonella multi-epitope protein is shown as SEQ ID NO.2.The nucleotide sequence of the coding gene of the Salmonella multi-epitope protein is shown as SEQ ID NO.1.The Salmonella subunit vaccine prepared by using the Salmonella multi-epitope protein has the advantages of safety and stability, low cost, no toxicity to the body, and the ability to simultaneously cause the body to produce humoral immunity and cellular immunity, and the like, and can not only produce a preventive protection effect on Salmonella enteritidis infection, but also can play a cross protection effect on Salmonella typhimurium infection.

The invention belongs to the technical field of microbial sterilization preparations, and particularly discloses a broad-spectrum salmonella bacteriophage SD40-16 and application of the broad-spectrum salmonella bacteriophage SD40-16. The bacteriophage SD40-16 can be used for cracking six serotypes of salmonella and escherichia coli including salmonella enteritidis and salmonella typhimurium, is wide in host range and strong in killing capability, and can be used for inhibiting the growth of bacteria. And meanwhile, the strain has good thermal stability and acid-base tolerance, and is easy to proliferate and enrich. The bacteriophage can enhance the antibacterial ability of kanamycin, provides a new drug combination strategy, has an obvious synergistic effect on salmonella resistance when being combined with kanamycin, and has a good application prospect in the aspect of preventing and treating salmonella infection.

The invention relates to the field of medicinal chemistry, and particularly discloses an emodin Mannich base as well as a preparation method and application thereof. The emodin Mannich base has a structure as shown in a formula I. The emodin Mannich base is simple in structure and has relatively strong in-vitro antimicrobial activity; the compound has very high inhibitory activity on bacteria such as staphylococcus aureus, bacillus subtilis, micrococcus luteus, escherichia coli, proteusbacillus vulgaris, pseudomonas aeruginosa, shigella dysenteriae and salmonella typhimurium, and fungi such as candida utilis, candida albicans, saccharomyces cerevisiae and monascus purpureus. The compound can be used for preparing antibacterial and / or antifungal drugs. # imgabs0 #

The application discloses Parabacteroides distasonis capable of inhibiting pathogenic bacteria and resisting tumors and an application thereof, relates to the technical field of microorganisms, and has the following technical points: the preservation number of Parabacteroides distasonis NGMCC 1.200926 is CGMCC No: 40687; the preservation address is No. 3, Xili Beichen, Chaoyang District, Beijing; the preservation unit is the General Microbiological Center of China Microorganism Culture Collection Committee; and the preservation time is July 5, 2023. After fermentation culture of Parabacteroides distasonis NGMCC 1.2000926, strong bacteriostatic active substances can be generated, the pathogenic bacteria such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa have obvious bacteriostatic effects, and the bacteriostatic active substances have good tumor cell activity, so that the application has a broad development space in the prevention and treatment of diseases caused by the pathogenic bacteria such as Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa and tumors, and has a good development and application prospect in antibacterial drugs and antitumor drugs.

The invention discloses a salmonella multi-epitope protein, a coding gene and application of the salmonella multi-epitope protein in preparation of a salmonella subunit vaccine, and relates to the technical field of biology. The amino acid sequence of the salmonella multi-epitope protein is as shown in SEQ ID NO. 2. The nucleotide sequence of the coding gene of the salmonella multi-epitope protein is as shown in SEQ ID NO. 1. The salmonella subunit vaccine prepared by utilizing the salmonella multi-epitope protein has the advantages of safety, stability, low cost, no toxicity to the body, capability of simultaneously causing the body to generate body fluid and cellular immunity and the like, not only can have a prevention and protection effect on salmonella enteritidis infection, but also can have a cross protection effect on salmonella typhimurium infection.

The invention discloses a lactoferrin modified florfenicol nano-liposome (R-FL) as well as a preparation method and application thereof. The R-FL is prepared by adopting a technology of combining an improved reversed-phase solvent evaporation method and chemical coupling. The R-FL disclosed by the invention is strong in slow and controlled release capability and good in biocompatibility, has excellent in-vitro and in-vivo antibacterial effects, and can be used for inhibiting the formation of a biofilm by precisely damaging the structure of the biofilm of the drug-resistant salmonella typhimurium in a targeting manner. The R-FL can effectively regulate the intestinal flora disorder caused by the drug-resistant salmonella typhimurium, so that the intestinal flora is recovered to a normal level. The R-FL can be used as a novel nano preparation with great potential for resisting drug-resistant salmonella typhimurium disease, and has a wide application prospect.

The invention provides a primer probe combination for detecting salmonella gallinarum, a kit and application, and belongs to the technical field of biological detection. In the primer probe combination, an upstream primer sequence of a primer is as shown in SEQ ID NO. 1; the sequence of a downstream primer of the primer is as shown in SEQ ID NO. 2; and the sequence of the probe is as shown in SEQ ID NO. 3. A primer probe combination is designed for a target gene, shown as SEQ ID NO.4, of salmonella gallinarum, the primer probe combination is high in salmonella gallinarum detection sensitivity, the sensitivity is 2.15 * 10 < 1 > copy number, and the salmonella gallinarum can be specifically detected from escherichia coli, salmonella pullorum, salmonella enteritidis and salmonella typhimurium. Meanwhile, the period of detecting the salmonella gallinarum by using the primer probe combination is short, and the operation detection and the result are easy to read.

The present invention discloses an anti-Salmonella typhimurium antibody, immunomagnetic beads labeled therewith, and applications thereof. The present invention provides an anti-Salmonella typhimurium monoclonal antibody comprising a light chain variable region and a heavy chain variable region, wherein LCDR1 of the light chain variable region comprises the amino acid sequence shown in SEQ ID NO:1, LCDR2 comprises the amino acid sequence LAS, and LCDR3 comprises the sequence shown in SEQ ID NO:2; and HCDR1 to HCDR3 of the heavy chain variable region comprise the sequences shown in SEQ ID NO:3, 4, and 5, respectively. The present invention establishes a Salmonella typhimurium immunomagnetic bead enrichment and separation method based on the specific monoclonal antibody against Salmonella typhimurium. The method is rapid and simple, has a high capture rate, strong specificity, and good sensitivity.