123 results about "Typhoid fever" patented technology

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against Fowl Typhoid causing Salmonella Gallinarum (SG) and Pullorum disease-causing Salmonella Pullorum (SP). Further, the present invention relates to a composition for the prevention or treatment of infectious diseases caused by Salmonella Gallinarum or Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to feed and drinking water for poultry, sanitizers and cleaners, comprising the bacteriophage as an active ingredient.

The invention discloses a healthcare bean dreg steamed bun. The healthcare bean dreg steamed bun is composed of the following raw materials, by weight, 65-75 parts of flour, 8-10 parts of oat powder, 5-7 parts of bean dreg, 6-10 parts of shiitake, 2-3 parts of shelled melon seed, 7-10 parts of hawthorn fruit powder, 2-3 parts of cassia seed, 1-2 parts of Radix Puerariae, 1-2 parts of Radix Platycodonis, 2-4 parts of Hibiscus sabdariffa, 2-4 parts of garlic, 2-3 parts of sunflower receptacle, 2-4 parts of pumpkin leaf, 2-3 parts of lily, 0.1-0.2 parts of mycose and 0.06-0.08 parts of a guar gum. The steamed bun produced in the invention has the advantages of fine and smooth taste, abundant mouthfeel, realization of waste utilization of substantially-saved production cost of added bean dregs, and reduction loss of nutritional components in the steaming process and enhancement of the healthcare values through the mix granulation of the bean dregs and other raw materials, and Radix Puerariae can invigorate splenic yang, release the flesh, promote eruption, stop diarrhea, relieve restlessness and quench thirst, and can be used for treating typhoid fever, warm headace and painful stiff nape, smothery sensation dysphoria and excessive eating symptom-complex, diarrhea, dysentery, uneruptive ecchymosis, hypertension, stenocardia, epicophosis and the like. The steamed bun disclosed in the invention has the efficacies of cancer preventing, ageing delaying, blood pressure reducing and the like.

The invention discloses dried tofu with black beans as main ingredients. The dried tofu comprises the following raw materials in parts by weight: 90-100 parts of black beans, 10-16 parts of corn kernels, 4-6 parts of boxthorn leaves, 3-5 parts of grape leaves, 2-4 pars of Chinese hawthorn leaves, 2-3 parts of betel leaves, 1-2 parts of the root of kudzu vine, 1-2 parts of rhodiola rosea, 2-3 parts of white sugar, 2-6 parts of salt, 0.5-1 part of aginomoto, a proper amount of waxberry and 0.03-0.06 part of a bean product defoaming agent. The dried tofu prepared by the invention has rich nutrition and good taste; the plurality of powder agent added before squeezing and the method of using the waxberry as flavor modifier provide high health protection value to the product; the waxberry has the effects of harmonizing stomach, preventing vomiting, producing saliva, slaking thirst, helping digestion and the like; the root of kudzu vine can raise Yang, relax muscles, promote eruption, check diarrhea, relieve restlessness and quench thirst; the dried tofu is used for treating the diseases such as typhoid fever, strong warm headache, vexation heat, thirst, diarrhoea, dysentery, ecchymosis, hypertension, angina and deafness.

The invention relates to a preparation technology for the blend oil of roots of kudzu vine and penoy seeds. The preparation technology comprises the following steps: firstly, cutting the roots of kudzu vine into slices in thickness of 0.1 to 0.3 cm, heating and steaming, then adding ethyl alcohol, carrying out ultrasonic treatment at a frequency of 2KHZ to 45 KHZ for 5 to 10 min, filtering, then mixing the filtrate with oil residue produced after single pressing, heating to a temperature of 35 DEG C to 50 DEG C and keeping the temperature for 15 to 20 min, then carrying out ultrasonic treatment at 30KHz to 40KHz for 5 to 10 min, and heating to a temperature of 35 DEG C to 50 DEG C and keeping the temperature for 15 to 20 min; then combining the mixture with crude oil produced after single pressing, then carrying out ultrasonic treatment again, after insulation treatment, carrying out rotary evaporation at a temperature of 60 to 65 DEG C, and completely volatilizing the ethyl alcohol, namely, obtaining primary oil, and then obtaining the blend oil of roots of kudzu vine and penoy seeds through corresponding treatments of deacidification, decoloration and deodorization. The prepared blend oil of the roots of kudzu vine and the penoy seeds can be in industrialized volume production, can serve as edible blend oil for long term use, has a better health caring effect, can lower blood pressure and resist cancer, and has better effects on typhoid fever, headache, dysphoria with smothery sensation and consumptive thirst, diarrhea diseases, dysentery, ecchymosis, stenocardia and epicophosis.

The present invention is drawn to multivalent Salmonella enterica serovar conjugate vaccines comprising conjugates of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi, S. Paratyphi A and optionally S. Paratyphi B, wherein the conjugates comprise a hapten antigen and a carrier antigen, wherein at least one of the hapten antigens or carrier antigens is characteristic of the Salmonella enterica serovar. The present invention also provides Salmonella enterica serovar reagent strains to produce the multivalent conjugate vaccines and attenuated Salmonella enterica serovars for use as vaccines.

The invention discloses a method for making massive ginger brown sugar, and relates to the field of edible sugar production. The method comprises the following preparation steps: putting crystal sugar syrup separated from monocrystalline and polycrystalline crystal sugar, and brown granulated sugar into a sugar dissolving pan; adding water to dissolve, filtering through a filter net and then conveying to a concentrating pan to carry out sugar boiling; removing excessive moisture to obtain molasses; controlling the pH value of the molasses by using citric acid and sodium bicarbonate, feeding the molasses into a sugaring car, simultaneously adding 5% of ginger powder and evenly stirring and sugaring the molasses by using an agitator; and putting on a sugar cloth of a work bench, and then marking off to obtain the massive ginger brown sugar. The method for making the massive ginger brown sugar disclosed by the invention is simple to operate and fewer in procedures, and the ginger brown sugar made by the method is convenient to eat, can be used for dispelling cold, and treating typhoid fever, headache and rhinobyon, and has good promotion and use value.

The invention discloses a seaweed-meal-containing chicken ham which is composed of the following raw materials, by weight, of chicken breast of 60-70 parts, ice water of 15-20 parts, seaweed meal of 10-15 parts, corn flour of 8-10 parts, soy protein of 6-8 parts, pumpkin of 3-5 parts, kudzuvine root of 3-5 parts, red date of 3-4 parts, xanthan gum of 1-2 parts, rehmannia flower of 1-2 parts, snake gourd betel pepper of 1-2 parts, sodium carboxymethylcellulose of 0.6-1 part, chicken flavor of 0.05-0.1 part, salt of 1.5-2 parts and monosodium glutamate and white granulated sugar of appropriate amount. The ham is wide in material, low in cost, good in taste and high in comprehensive nutritive value, the added kudzuvine root can invigorate splenic yang, release the flesh, promote eruption, stop diarrhea, relieve restlessness and quench thirst, and the ham can be mainly used for treating typhoid fever, headache, dysphoria with smothery sensation, diarrhea, dysentery, ecchymosis, hypertension, angina and epicophosis and other diseases and quenching thirst.

The invention discloses a moxibustion plaster and the process for preparation, wherein the plaster comprises the following constituents (by weight percent): hot pepper 5. 0-30. 0%, Cinnamomum cassia 0. 5-10. 0%, fresh ginger 58. 0-85. 0%, oil of Chinese cinnamon 0. 0002-0. 005%. The plaster can be used for treating arthralgia due to cold, limb and knuckle crymodynia, typhoid fever, cold of insufficiency type, chronic rheumatic arthritis and chronic enteritis.

The invention belongs to the field of biotechnology and medical science, and provides a typhoid fever salmonella and salmonella paratyphi fluorescent quantitative polymerase chain reaction (PCR) detection kit. The detection kit comprises a first reaction solution, a second reaction solution and paraffin, wherein the first reaction solution comprises a reaction buffer solution, a primer pair and an ecprobe aiming at typhoid fever salmonella, a primer pair and an ecprobe aiming at the salmonella paratyphi. The sequence of the primer pair aiming at the typhoid fever is SEQ ID NO: 1 and SEQ ID NO: 2, the sequence of the ecprobe aiming at the typhoid fever is SEQ ID NO: 3, the sequence of the primer pair aiming at the salmonella paratyphi is SEQ ID NO: 3 and SEQ ID NO: 3, and the sequence of the ecprobe aiming at the salmonella paratyphi is SEQ ID NO: 6. The second reaction solution comprises deoxyribonucleic acid (DNA) polymerase O. The invention further provides an application of the detection kit in detection of the typhoid fever salmonella and salmonella paratyphi. According to the detection kit, the detection time is shortened, cross contamination among samples is avoided, and the result is accurate and reliable.

The present invention provides an oral vaccine against a bacterial infectious disease (e.g., typhoid fever, cholera, or dysentery). The oral vaccine of the present invention is a capsule formulation in which a transformed microorganism that expresses a flagellin antigen protein or that secretes a flagellin antigen protein out of the cell of the microorganism is encapsulated with an acid-resistant membrane. Examples of the microorganism include intestinal bacteria belonging to the genus Bifidobacterium, the genus Lactobacillus, the genus Lactococcus, and the like. The form of the capsule formulation may be any one of a seamless capsule formulation, a soft capsule formulation, and a hard capsule formulation.

The invention discloses chicken bone beatifying vermicelli which is composed of, by weight, 92-100 parts of corn flour, 20-28 parts of wheatmeal, 14-20 parts of mulberry, 11-15 parts of chicken bones, 2-3 parts of pseudo-ginseng, 2-3 parts of Chinese angelica, 1-3 parts of the fruit of wolfberry, 3-4 parts of angelica root, 4-6 parts of sunflower discs, 2-3 parts of the root of kudzu vine, 1-2 parts of juncus effusus, 1-2 parts of dragon's tongue leaves, 2-3 parts of snakegourd fruit leaves, 2-3 parts of grape leaves, edible salt and water in proper amount. The chicken bone beatifying vermicelli is smooth in mouthfeel, and added animal bones are subjected to decoction with traditional Chinese medicine and added with citric acid, so that separation of active ingredients like calcium in the bones is facilitated, absorption by human bodies is facilitated, collapsing force to nutrient in the chicken bones is small, nutrient substances in the bones can be fully retained in products, and human cell tissue is protected to a certain extent; the root of kudzu vine has functions in promoting Yang and releasing muscle, promoting eruption and diarrhea inhibiting, and relieving restlessness and quenching thirst, and can be used for treating diseases like typhoid fever, tepidity, headache and rigid neck, vexed heat and thirst elimination, diarrhea, dysentery, opague macula, hypertension, angina and deaf, and the corn flour contains a great amount of lecithin, linoleic acid, grain alcohol, vitamin E, cellulose and the like, and has various healthcare effects of maintaining beauty and keeping young, delaying aging and the like.

The invention discloses an injection for treating the paratyphoid of dogs and a preparation method thereof, which aim to provide an injection which has quick effect and can reduce the drug resistant probability of pathogenic bacteria, treat both symptoms and root causes and quicken the recovery, and a preparation method which has simple process and is easy to realize. Per 100L of the injection comprises 2-15kg of sulfamonomethoxine sodium, 1-10kg of lincomycin hydrochloride, 0.05-0.3kg of atropine sulfate, 0.06kg of dexamethasone sodium phosphate, 0.2kg of sodium bisulfite, 0.01kg of EDTA-2Na and the balance of water. The injection adopts antibacterial drugs, antibiotics, glucocorticoids for diminishing inflammation and detoxifying and atropine sulfate for controlling the diarrhea of the dogs and reducing dehydration, and a compound preparation prepared by reasonably proportioning the dosages of the components has the function of double anti-bacteria, can reduce the drug resistant probability of salmonella typhimuria, control the infection of other gram negative bacteria and positive bacteria and accelerate the recovery and has high cure rate.

The invention discloses primers, probes, a test kit and a test method for triple real-time fluorescence PCR detection of Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum. According to the present invention, a triple real-time PCR method is used, wherein a consensus sequence of Salmonella choleraesuis and Salmonella paratyphi is used for the detection of Salmonella choleraesuis and Salmonella paratyphi, a specific sequence of Salmonella typhi is used for the detection of Salmonella typhi, and a specific sequence of Salmonella gallinarum is used for the detection of Salmonella gallinarum. Whether a sample is contaminated by Salmonella choleraesuis, Salmonella paratyphi, Salmonella typhi, and Salmonella gallinarum is determined via a real-time fluorescence PCR amplification, and then Salmonella choleraesuis and Salmonella paratyphi are distinguished via a single fluorescent PCR amplification; the detection is rapid, the process of preparing sample and issuing test results can be finished in 31hours, the results are reliable, and sensitivity and specificity are high.

The invention discloses a molecular subtyping method for salmonella typhimuria and a specific SNP locus combination. The invention provides an application of a substance for detecting 16 SNP loci nucleotide in a genome of a to-be-detected salmonella typhimurium in preparing a product for detecting or auxiliarily detecting the molecular subtyping condition of the salmonella typhimuria, or an application of a substance for detecting 16 SNP loci nucleotide in a genome of a to-be-detected salmonella typhimurium in preparing a product for detecting or auxiliarily detecting the molecular subtyping condition. The experiment verifies that the research and development sample amount exceeds 6000for the clinically common blood serum salmonella typhimuria for molecular subtyping to obtain an SNP locusset of the molecular subtyping. Corresponding probe can be designed by using the set. The salmonella typhimuria are quickly typed in experiment. The method is short in time and low in consumable. Inaddition, the mouse typhus salmonella genotyping difficulty is greater than of the salmonella typhimuria, so that research and development of the molecular subtyping for mouse typhus salmonella is ofextremely value.

The invention relates to a method for detecting adenosine deaminase activity through liquid chromatography tandem mass spectrometry and screening an inhibitor thereof. The method comprises the following steps: reacting a sample (whole blood, serum or hemocyte) to be detected and substrate adenosine, or reacting the sample (traditional Chinese medicine or compound) to be detected, an adenosine deaminase and substrate adenosine; then detecting inosine content in the stop solution by the liquid chromatography tandem mass spectrometry; and calculating the activity of adenosine deaminase. The method has the advantages of being high in accuracy, high in precision, high in sensitivity, high in stability, simple to operate, low in cost, and high in throughput; an effective method is provided to clinically detect the adenosine deaminase activity and diagnose leukemia, typhoid fever, systemic lupus erythematosus, diabetes, hepatopathy and tumor, and the support is provided to screen the inhibitor of the adenosine deaminase in order to treat the abovementioned diseases.

The present invention provides an extraction method for outer membrane vesicles (OMVs) of salmonella typhimurium. The extraction method comprises the following steps: (1) salmonella typhimurium liquidis subjected to centrifuging at 10,000 x g and 4 DEG C for 15 min; (2) a supernatant is recovered, and a salmonella typhimurium liquid precipitate is not poured out and subjected to centrifuging at 13,000 x g and 4 DEG C for 15 min; (3) the supernatant is recovered and filtering is conducted twice with a PVDF membrane filter; and (4) the filtered supernatant is subjected to centrifuging at 40,000x g and 4 DEG C for 2 h; and the supernatant is discarded. The applied high-speed centrifugation scheme successfully extracts the OMVs of the salmonella typhimurium with a centrifugal force of 40,000x g. The extraction method also solves a problem that flagella are abundantly present in the OMVs extract. A gene targeting editing technique is applied to knock out flagella genes of the salmonellatyphimurium to obtain the high-purity OMVs.