The invention discloses a
salmonella H: i
epitope specific
monoclonal antibody and application thereof, the
salmonella H: i
epitope specific
monoclonal antibody is secreted by hybridoma cells with the preservation number of CCTCC NO: C202645, and the
antibody has the advantages of
high titer and strong specificity. According to the blocking ELISA detection kit constructed by the antibody and the detection method of the blocking ELISA detection kit, the antibody containing H: i
antigen epitope salmonella in infected animal serum can be specifically detected, the antibody does not react with antibodies containing no H: i
antigen epitope salmonella and non-salmonella, the sensitivity, specificity and stability are good, and the detection method is simple and convenient. And a new method is provided for direct serological detection of H: i
antigen epitope-containing
salmonella infection of mouse typhimurium, kafenkii and the like.