83 results about "Salmonella infection" patented technology

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The invention provides an application of thymol to preparation of medicines for treating salmonella infection. By using a human cervical carcinoma cell (Hela) as an infection carrier, gentamicin protective experiments, in vitro biochemical experiments and mouse salmonella infection models all verify that thymol has obvious inhibiting effects on salmonella infection by affecting the functions of secretory systems III. Antibiotics easily induce generation of medicine resistance in the process of treating salmonella infection. Compared with the antibiotics, the traditional Chinese medicine compound thymol can directly inhibit the functions of the salmonella secretory systems III and play a role in resisting infection, does not easily induce generation of medicine resistance and has the characteristic of high cure rate.

The present invention relates to a composition comprising bacteriophage SP-1, the bacteriophage capable of destroying Salmonella once being infected in Salmonella, as an active ingredient, and a method for prevention and treatment of Salmonella infection using the same. Bacteriophage SP-1, the active ingredient of the composition of the present invention, characteristically has the killing activity to Salmonella and has the genome represented by SEQ. ID. NO: 1.

The invention discloses a preparation method for a polyclonal antibody to salmonella effect protein SopB. The invention aims to provide the preparation method for the rabbit-derived polyclonal antibody with good specificity, high sensitivity and capability of specifically binding to the endogenous salmonella effect protein SopB secreted in the process of salmonella infection of a host cell. A protein sequence as represented by SEQ ID No. 2 is used as immunogen, wherein the protein sequence consists of 139 amino acids located between position 29 and position 168, counted from terminal N, of a full-length amino acid sequence as represented by SEQ ID No. 1 of the salmonella effect protein SopB, and a conventional preparation method for a polyclonal antibody is used for preparation of antiserum; then the antiserum is purified so as to obtain the polyclonal antibody to the salmonella effect protein SopB. According to the invention, the polyclonal antibody to the salmonella effect protein SopB is prepared by using the method, and the polyclonal antibody is beneficial for immunoblotting analysis and immunofluorescent staining laser confocal microscopic observation of the salmonella effect protein SopB and for in-depth research on the infection mechanism of enteropathogens.

The present invention provides a vaccine composition, which contains a combination of Salmonella typhimurium microorganisms of gene deletion mutants and Escherichia coli microorganisms of gene deletion mutants, and is suitable for mass application to poultry. The invention also provides a safe and effective method for protecting poultry from Escherichia coli and Salmonella infection and disease damage.

The present invention relates to Salmonella mutant strains and their use as a vaccine for preventing Salmonella infection, in particular in eggs.

The invention discloses a caenorhabditis elegans infection model for high-throughput rapid detection of salmonella virulence and a preparation method and application thereof, and belongs to the technical field of biology. The model is characterized in that: the caenorhabditis elegans is sek-1, glp-1 gene defect type caenorhabditis elegans; the culture medium for the salmonella infected caenorhabditis elegans is an S-medium buffer solution with a pH value of 7.0 + / -0.2; and the infection concentration of the salmonella is 10 < 7 >-10 < 11 > CFU / mL. The survival rates of the nematodes are obviously different when the nematodes are infected at the second to the fourth time, and the pathogenicity is preliminarily judged. Therefore, by establishing a salmonella infected caenorhabditis elegans model, the salmonella virulence can be quickly, simply, conveniently and efficiently detected, and a theoretical basis is provided for salmonella pathogenesis research and related drug screening.

The invention relates to a colistin sulfate and berberine hydrochloride composition and a preparation method of pellets of the colistin sulfate and berberine hydrochloride composition and aims to solve the problems that the effect of treating escherichia coli and salmonella infection by using the colistin sulfate is poor due to poor intestinal absorption in the prior art, and the use dosage is required to be increased for reaching an ideal treatment effect. The preparation method comprises the following steps: firstly, mixing the colistin sulfate, the berberine hydrochloride, a filling agent, a lubricating agent and a disintegrating agent; secondly, crushing to obtain mixture powder; adding a binding agent to obtain pellets; coating to obtain the pellets of the colistin sulfate and berberine hydrochloride composition. The pellets of the colistin sulfate and berberine hydrochloride composition, obtained by the preparation method disclosed by the invention, are prepared by adopting conventional equipment such as a granulating device and a coating device in the pharmaceutical industry; the preparation method can realize continuous production and is suitable for industrial production; high yield is realized.

The invention provides a plant Lactobacillus (Lactobacillus plantarum) resistant to Salmonella enteritidis infection and application thereof, belonging to the technical field of microbes. The bacterium was deposited in the General Microorganism Center (CGMCC) of the China Committee for the Collection of Microorganisms on September 24, 2015, and the preservation number is CGMCC No.11446. The Lactobacillus plantarum CGMCC No. 11446 of the present invention has better properties of acid resistance and bile salt resistance, and has significant inhibitory effect on the growth of Salmonella enteritidis. It has a strong inhibitory effect on the adhesion of Salmonella Enteritidis to intestinal epithelial cells HT-29, and has the effect of preventing or alleviating Salmonella Enteritidis infection. The Lactobacillus plantarum CGMCC No. 11446 can be used to prepare pharmaceutical compositions for preventing or alleviating Salmonella enteritidis, food and fermented food containing active lactic acid bacteria, and has very broad application prospects.