Avian combination-vaccine against e.coli and salmonella

A technology for Escherichia coli and poultry, applied in the field of combined poultry vaccines

Inactive Publication Date: 2006-06-14
WYETH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although live E. coli vaccines are available commercially against colibacillosis in turkeys, these are not all suitable vaccines for use in chickens
Also, there is no combination vaccine available that is safe and effective against Salmonella and E. coli infection in poultry including chickens and that is economically administered, eg, by spraying or drinking water in large quantities on chickens after hatching

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Construction of Escherichia coli Mutant with AROA Gene Deletion

[0030] I) Receptor

[0031] The parental organism was an avian E. coli isolate isolated from a clinical case of avian colibacillosis submitted to the Veterinary Laboratories Agency (VLA), Addlestone, Surrey, UK in 1995 and serotyped at the VLA. The parental strain was selected for its characteristics of persistence, invasiveness, persistence and pathogenicity in day-old SPF chickens, and antibiotic susceptibility in vitro. Recipient strains were generated by combining transformed donor (E. coli K12 S17λpir carrying PNG101, deletion of aroA carrying 100 bp) and wild-type parental strain (wild-type E. coli isolate EC34195).

[0032] II) Missing features

[0033] The aroA gene, which encodes 3-phosphoenolpyruvylshikimate-5-phosphate synthase, an enzyme of the general aromatic biosynthetic pathway, is located at the end of the promoter of serC adjacent to the serC-aroA operon. Deletion of the aroA gene in ...

Embodiment 2

[0047] Preparation of master species

[0048] Escherichia coli aroA-strain (constructed in Example 1) was cultured once on a typical soybean agar plate and then three times in a typical soybean broth. Cultures were distributed into glass vials, sealed and lyophilized.

Embodiment 3

[0050] Evaluation of the efficacy of combinations of E. coli gene-deleted mutant microorganisms and Salmonella typhimurium gene-deleted mutant microorganisms against E. coli and Salmonella infection in chickens

[0051] In the evaluation, the Escherichia coli gene-deleted mutant microorganism used was the Escherichia coli aroA-strain described in Examples 1 and 2, and the used Salmonella typhimurium gene-deleted mutant microorganism was prepared as described in US 6,231,871 B1 Salmonella typhimurium STM-1.

[0052] For evaluation, 103 SPF white Leghorn chickens, regardless of sex, were divided into 5 groups, 2 groups of 25 birds each, 2 groups of 24 birds each and 1 control group (no vaccination, no change) of 5 birds. Chickens with their wings removed were forced to be placed in designed isolation chambers. Each experimental group was housed in two isolation rooms with 12 or 13 chickens in each isolation room.

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PUM

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Abstract

The present invention provides a vaccine composition, which contains a combination of Salmonella typhimurium microorganisms of gene deletion mutants and Escherichia coli microorganisms of gene deletion mutants, and is suitable for mass application to poultry. The invention also provides a safe and effective method for protecting poultry from Escherichia coli and Salmonella infection and disease damage.

Description

field of invention [0001] The present invention relates to a combined poultry vaccine against Salmonella and Escherichia coli infection. In particular, the present invention relates to vaccine compositions for poultry containing mutant and live attenuated strains of Salmonella and Escherichia coli, respectively. The present invention also relates to a method of preventing bacterial disease in poultry, including chickens, by administering a vaccine comprising attenuated microorganisms of Salmonella and Escherichia coli. Background of the invention [0002] In the poultry industry, newly hatched chickens are particularly susceptible to Salmonella infection. This bacterium is spread through feces, through which young animals may become infected or perhaps through contaminated processed feed, which leads to high mortality with serious economic consequences. In addition to Salmonella, the environment of poultry houses contaminated by excreta also contains large numbers of Esche...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/112A61K39/02A61K39/108A61P31/04A61P37/04C12N1/21C12N9/10C12N15/03C12R1/19C12R1/42
CPCA61K39/0258A61K39/0275A61K2039/522A61K2039/552C12N9/1092A61P31/04A61P37/04Y02A50/30
Inventor H·H·范M·库马
Owner WYETH LLC
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