The invention discloses a Bim (Bcl-2 interacting
mediator of
cell death)
gene deletion fluorescent quantitative PCR (
polymerase chain reaction) detection primer, a probe and a detection
reagent kit, and belongs to the field of molecular biological detection. The Bim
gene deletion fluorescent quantitative PCR detection primer and the probe comprise forward primers 5'-CAACAAACCCATCAGAACAGACAC-3', reverse primers 5'-ACAGCCTCTATGGAGAACAGTGATT-3 and fluorescent probes 5'-FAM-CAGACACTGGAACAAA-MGB-3'. The detection
reagent kit comprises the Bim
gene deletion fluorescent quantitative PCR detection primer, the probe, premixed liquid of PCR liquid and
positive control samples A. The Bim
gene deletion fluorescent quantitative PCR detection primer, the probe and the detection
reagent kit have the advantages that Bim
gene deletion mutation can be detected by the aid of the Bim
gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit, and the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit are simple, convenient and feasible and are high in accuracy and sensitivity and particularly suitable for detecting clinical samples; Bim
deletion mutation can be detected, and accordingly the Bim gene deletion fluorescent quantitative PCR detection primer, the probe and the detection reagent kit can bring convenience for guiding individual treatment on bodies.