Seneca recombinant virus of recombinant A-type foot-and-mouth disease virus VP1 gene, recombinant vaccine strain and preparation method and application of recombinant vaccine strain

A foot-and-mouth disease virus and recombinant vaccine technology, applied in the field of Seneca recombinant virus, can solve problems such as indistinguishability, and achieve the effects of reducing pathogenicity, improving biological safety, and ensuring pertinence
CN111996201AActive Publication Date: 2020-11-27LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Publication Date
2020-11-27

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Abstract

The invention provides a Seneca recombinant virus of a recombinant A-type foot-and-mouth disease virus VP1 gene, a recombinant vaccine strain and a preparation method and application of the recombinant vaccine strain, and relates to the technical field of gene engineering. The invention provides a Seneca recombinant nucleic acid, the Seneca recombinant virus containing the recombinant nucleic acid, the Seneca recombinant vaccine strain containing the Seneca recombinant virus, and a preparation method and application of the vaccine strain. An SVV / FJ / 001 strain is subjected to gene deletion mutation transformation, the VP1 gene of the A-type FMDV is fused into cDNA of the SVV / FJ / 001 strain to obtain the Seneca recombinant virus, the recombinant virus can express the fused gene, and an expression product has good reactogenicity; and the obtained vaccine strain is high in antigen productivity, the pathogenicity is remarkably reduced, even no pathogenicity is caused to pigs, the immune response of SVA can be stimulated after animals are immunized by the inactivated vaccine, the immunocompetence aiming at fusion genes can be generated, and the vaccine strain can be used for preventing and controlling Seneca virus and one or more non-Seneca virus.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a Seneca recombinant virus recombined with the VP1 gene of type A foot-and-mouth disease virus, a recombinant vaccine strain and a preparation method and application thereof. Background technique

[0002] Senecavirus A (Senecavirus A, SVA), also known as Senecavirus (Senecavirus), Seneca valley virus (Seneca valley virus, SVV), belongs to the small RNA virus family (Picornaviridae) Senecavirus genus (Senecavirus) ), the only member of the genus. The virus infects pigs and can cause primary vesicular disease in pigs, which is indistinguishable from clinical symptoms caused by foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis. SVA infection can cause vesicular lesions in weaned piglets, nursery, fattening and breeding pigs of all ages, accompanied by clinical symptoms such as lameness, fever, anorexia, lethargy, etc. In addition to t...

Claims

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