15 results about "Reactogenicity" patented technology

The invention relates to the technical field of biological pharmacy, and discloses a QS-21 saponin adjuvant as well as a preparation method and application thereof, the adjuvant is a composite liposome and comprises a liposome skeleton composed of distearoyl phosphatidylcholine and cholesterol, and QS-21 saponin, monophosphoryl lipid A and a local anesthetic are jointly entrapped in the liposome skeleton. The problem that high reactogenicity and immunogenicity of potent adjuvants are difficult to consider at the same time is solved, the immunostimulation component and the pain inhibition component are jointly entrapped in the same nano-carrier, collaborative delivery in injection local is achieved, and therefore pain and swelling caused by the adjuvants are accurately inhibited. The co-entrapment structure avoids the potential inhibition effect of the free anesthetic on the immune system, the potent body fluid and cellular immune enhancement activity of the adjuvant is completely reserved, and a new technical scheme is provided for developing vaccines with high safety and strong immune efficacy.

The invention belongs to the technical field of fish immunodetection, and particularly relates to antigenic epitope polypeptide of anti-channa argus IgM, a polyclonal antibody as well as a preparation method and application of the polyclonal antibody. Specifically, the invention successfully constructs a preparation technology system based on the snakehead IgM specific antigen epitope polypeptide and the corresponding polyclonal antibody. Meanwhile, through combination of bioinformatics epitope prediction and immunogenicity optimization, the linear epitope polypeptide with high reactogenicity is successfully screened and obtained. And finally constructing to obtain the anti-channa argus IgM polyclonal antibody, and the verification shows that the polyclonal antibody has high specific recognition capability on channa argus mucus IgM protein. The successful preparation of the antibody fills the blank of a reagent for detecting the mucosal immune globulin of the freshwater economic fish, and provides a core reagent basis for establishing standardized detection platforms such as fish vaccine titer evaluation and immune response dynamic monitoring, so that the antibody has good practical application value.

The invention discloses a broad-spectrum avibacterium paragallinarum protective antigen and application thereof, and belongs to the field of prevention and control of poultry infectious diseases. The invention provides an application of an avibacterium paragallinarum protein p1 in preparation of a broad-spectrum avibacterium paragallinarum protective antigen protein. The amino acid sequence of the avibacterium paragallinarum protein p1 is as shown in SEQ ID No. 1. The protective antigen protein has strong immunogenicity and reactogenicity, and a subunit vaccine prepared by using the antigen protein can provide 90% protection rate for avian paragallinarum of three serotypes A, B and C, and can effectively prevent infection of the avian paragallinarum.

The invention belongs to the field of biology, and discloses a coating protein for diagnosis of an African swine fever virus antibody, application and a kit, and the coating protein comprises a p22 protein and an E199L protein; the amino acid sequence of the p22 protein is as shown in SEQ ID NO: 1, and the amino acid sequence of the E199L protein is as shown in SEQ ID NO: 3. The coating protein is composed of recombinant proteins of p22 and E199L, the two recombinant proteins can react with ASF positive serum and have good reactogenicity, and the detection kit based on the coating protein is high in detection precision, strong in specificity and good in repeatability.

The application discloses a broad-spectrum protective antigen of avian pathogenic bacterium and application thereof, and belongs to the field of poultry infectious disease prevention and control. The application provides application of avian pathogenic bacterium protein p1 in preparation of a broad-spectrum protective antigen protein of avian pathogenic bacterium, and the amino acid sequence of the avian pathogenic bacterium protein p1 is shown as SEQ ID No. 1. The protective antigen protein has strong immunogenicity and reactivity, and a subunit vaccine prepared by using the antigen protein can provide 90% protection rate of chickens to A, B and C three serotypes of avian pathogenic bacterium, and can effectively prevent infection of the avian pathogenic bacterium.

The application belongs to the technical field of biology and specifically relates to a kind of nanobody targeting BVDV-E0 protein and application thereof.In detail, the nanobody of the application is a kind of nanobody with specificity and high binding force to BVDV-E0 protein and BVDV virus, which has good reactivity with antigen protein or virus, and the binding sensitivity can reach 1:1000, so it can be expected that the nanobody of the application has good application prospect in blocking E0 protein-mediated viral invasion, accurate virus diagnosis and treatment.

The application belongs to the field of biology, and discloses a coated protein, application and kit for diagnosing African swine fever virus antibody, wherein the coated protein comprises p22 protein and E199L protein; the amino acid sequence of the p22 protein is shown as SEQ ID NO:1, and the amino acid sequence of the E199L protein is shown as SEQ ID NO:3. The coated protein is composed of recombinant proteins of p22 and E199L, and both of the two kinds of recombinant proteins can react with ASF positive serum, and both have good reactivity. The detection kit based on the above coated protein has high detection precision, strong specificity and good repeatability.

The present invention belongs to the technical field of pathogenic bacterial disease prevention and treatment, and specifically relates to a fusion antigen of the N-terminus of IRP protein of Haemophilus parasuis and RLPB protein and its application. The amino acid sequence of the fusion antigen is shown in SEQ ID NO: 1. The reactivity of the fusion antigen is identified by Western-Blot, and it can be known that the fusion protein antigen can be recognized by the positive serum of Haemophilus parasuis antibody; and the indirect ELISA method constructed based on the fusion antigen has excellent specificity, sensitivity and repeatability for the positive serum of Haemophilus parasuis antibody; in addition, immunizing guinea pigs with the fusion antigen can obtain the protection effect against the attack of Haemophilus parasuis type 1 strain, type 5 strain and type 13 strain.

The application belongs to the technical field of biology and particularly relates to a bovine viral diarrhea virus NS2 truncated protein and application thereof in BVDV antibody detection. The application provides a truncated protein of BVDV NS2 protein. The truncated protein is soluble expressed after being fused with glutathione S transferase (GST) and horseradish peroxidase (HRP) respectively, has good reactivity with BVDV antibody positive serum, does not react with BVDV antibody negative serum, and can be used for BVDV antibody detection. Furthermore, the application constructs a double antigen sandwich ELISA method for BVDV antibody detection by taking the fusion protein of GST and the truncated protein as a coating antigen and taking the fusion protein of HRP and the truncated protein as a detection antigen. The method has the technical advantages of high sensitivity, good specificity, simple operation, easy popularization, low cost and the like, and can be applied to screening of BVDV antibodies in a cattle herd.

The disclosure describes compositions containing PEGylated compounds using linkers, bivalent polysaccharide covalent PEG compounds, and methods of bivalent polysaccharide-PEG compounds in the development of multivalent vaccines. PEGylated conjugation of capsular polysaccharides to carrier proteins is carried out using homo-bifunctional and / or hetero-bifunctional linkers of specific lengths. Incorporation of bifunctional PEG linkers induces higher titers of functional antibodies with high avidity, eliciting higher immunologic memory, and reduced carrier protein effect. This provides immunochemically cross-reactive capsular polysaccharides wherein one or more cross-reactive capsular polysaccharides are covalently PEG compounded sequentially or concurrently to carrier protein using bifunctional linkers bearing the same or different functional groups. Such a linker and the size of the capsular polysaccharides provides an effective multivalent vaccine with high antibody titers and a reduced carrier effect, with a reduction in the content of the capsular polysaccharide and protein per dose of vaccine which reduces reactogenicity.

The present invention discloses a recombinant protein, a kit and an ELISA detection method for detecting serotype 4 avian adenovirus. Specifically disclosed are a recombinant protein 100K (672-1053) having an amino acid sequence of SEQ ID No. 1 and its use as a detection antigen in the preparation of a product for detecting serotype 4 avian adenovirus. The present invention also discloses a FAdV-4 antibody detection kit and an ELISA detection method. The recombinant protein of the present invention specifically binds to FAdV-4 positive serum and has good reactivity. The detection kit and method established using the recombinant protein have strong specificity, no cross-reaction, high sensitivity, and good repeatability. At the same time, it can distinguish between animals immunized with inactivated vaccines and animals naturally infected to produce antibodies, and can identify FAdV-4 infection, provide technical support for the purification of FAdV-4, and provide reliable and effective means for the prevention and control of HHS diseases.

The invention provides chicken infectious anemia virus recombinant VP2 protein and application thereof in virus detection, and belongs to the technical field of virus detection. The invention provides a chicken infectious anemia virus recombinant VP2 protein, and the VP2 gene is highly conserved in each strain. When the recombinant VP2 protein is used as a coating antigen for detecting the CIAV antibody, the recombinant VP2 protein has good reactogenicity, specificity, sensitivity and repeatability. The kit can be used for clinical CIAV serum antibody detection, SPF chicken screening, exogenous virus antibody detection of poultry live vaccines or virus seeds, epidemiological investigation and the like, so that CIAV infection transmission is prevented and controlled.

The disclosure provides compositions, methods of treatment, and methods of making and using compositions to deliver a nucleic acid to a subject that, optionally, have reduced reactogenicity and promotes a local innate immune response in the subject while promoting an adaptive immune response. Compositions described herein include nanoparticles, optionally including an inorganic particle, capable of admixing with nucleic acids encoding proteins, antibodies, or immunomodulators. Methods of using the compositions as a therapeutic vaccine for the treatment of an infection or cancer are also provided.

Triazine lipids and solid-phase synthesis methods for synthesizing triazine lipids are provided. Certain triazine lipids disclosed can inhibit canonical NFKB transcriptional activity and thus may be utilized in non-viral transfection vectors for administration with an immunostimulatory antigen to reduce reactogenic response invoked by the immunostimulatory antigen. Methods for inhibiting canonical NFKB transcriptional activity during an immune response are also provided.

The application discloses a kind of detection anti-Brucella antibody multi-link B cell epitope recombinant protein and its application, it belongs to the field of biotechnology and immunodiagnosis technique, wherein, multi-link B cell epitope recombinant protein is formed by the B cell epitope of seven key outer membrane proteins Omp10, Omp16, Omp19, Omp25, Omp31, Omp2b, BP26 of Brucella, its amino acid sequence is as shown in sequence table SEQ ID NO:1.The multi-link B cell epitope recombinant protein has good antigenicity and reactivity, and the deer serum indirect ELISA detection system established with it as coating antigen shows excellent detection performance, has high sensitivity, strong specificity and good repeatability, and can accurately detect the level of anti-Brucella antibody in deer serum sample.