32results about How to "Strong green fluorescence" patented technology

The invention relates to an epitope peptide H362 of an HN protein in PPRV, and determination, a preparation method and application thereof. The amino acid sequence of the epitope peptide is H362: <362>EANWVVPSTDVRDL<375>. The invention detects reactogenicity of a monoclonal antibody and PPRV and specificity of the monoclonal antibody; according to detection results, the monoclonal antibody has good reactogenicity to rPPRV-HN-F protein; immunoinformatic technology is cooperatively used for predicating the B cell epitope of the HN protein; an aminated ELISA plate is employed for detecting candidate epitopes and the monoclonal antibody 10E3, and the epitope peptide H362 corresponding to 10E3 is determined; and determination of the epitope peptide lays a theoretical foundation for preparation of epitope vaccine antigens and diagnostic reagent antigens for PPRV.

The invention discloses a reverse transcription loop-mediated isothermal amplification (RT-LAMP) visual kit for detecting Japanese B encephalitis virus and application of the kit. The visual kit contains a primer group and color development substances, wherein primers have sequences shown as SEQ ID NO.1-4, and the color development substances are calcein and manganese chloride. A using method of the kit comprises the following steps of: preparing an RT-LAMP system containing AMV reverse transcriptase, a 1 time reaction buffer solution, strand displacement DNA polymerase, a dNTP mixture, betaine, calcein, manganese chloride, MgSO4, a forward inner primer (FIP), a backward inner primer (BIP) group, an F3 primer, a B3 primer and RNA of a sample to be detected; performing thermostatic reaction on the reaction system at the temperature of between 61 and 65 DEG C, and inactivating; and judging a result under natural light or ultraviolet or natural light and ultraviolet, wherein if the reaction product is green under the natural light, the sample to be detected contains the Japanese B encephalitis virus; and if the reaction product represents obvious green fluorescence under the ultraviolet, the sample to be detected contains the Japanese B encephalitis virus.

The invention relates to a lead-free all-inorganic indium-based zero-dimensional perovskite nanocrystal and a manufacturing method thereof, the chemical formula of the lead-free all-inorganic indium-based zero-dimensional perovskite nanocrystal is Rb3nCs3-3nInX6, the Rb3nCs3-3nInX6 is doped with antimony, the Rb3nCs3-3nInX6 is reacted to form Rb3nCs3-3nInX6: Sb, X is Cl or Br, and n is more than or equal to 0 and less than or equal to 1. The corresponding nanocrystal is obtained through the steps of raw material adding, variable-temperature treatment, centrifugal treatment and the like. According to the invention, a small amount of antimony element is used for doping, so that the prepared lead-free all-inorganic indium-based perovskite nanocrystal has very strong green fluorescence, the fluorescence quantum yield is up to 45.3%, and the lead-free all-inorganic indium-based perovskite nanocrystal is not only suitable for electroluminescence in a light-emitting diode, but also suitable for a fluorescent layer of a green light-emitting diode, and has a very good photoelectric application prospect.

The invention relates to peste des petits ruminant virus HN protein epitope peptide. The amino acid sequences of the epitope peptide are as follows: H123: <123>KFLNPDREYDFRDLR<137>, or/and H185: <185>GTGCLGRTVTRA<196>, or/and H487: <487>IRGPRGRCH<495>, or/and H569: <569>ECFPWYHKVWCYHDCLI<585>. B cell epitopes of a target protein are predicted by virtue of multiple immunoinformatic software, the different predicted epitopes are respectively artificially synthesized, the reactogenicity of the predicted epitopes is verified by virtue of an indirect ELISA method, an aminated ELISA Plate is coated with different polypeptides, and the reactogenicity with an antibody of HN protein is detected, and therefore, the B cell epitopes of the PPRV HN protein are authenticated.

The invention discloses a gold ion fluorescence probe, a preparation method and an application thereof. The fluorescence probe has high selectivity and high sensitivity, can be used for detecting gold ions. The gold ion fluorescence probe is a 4,5-dihydrazine pyridyl fluorescein derivative shown in a formula I, the preparation method comprises the following steps of reacting 4,5-fluorescein bi-aldehydes and hydrazinopyridines in an ethanol solution to generate the dihydrazine pyridyl fluorescein derivative which can be taken as the gold ion fluorescence probe.

The invention provides a green fluorescent diamine oxazoline zinc complex as well as a preparation method and application thereof, and belongs to the technical field of catalysis of luminescent materials. The complex can be obtained by direct reaction and purification of diamine oxazoline ligands containing different substituent groups and diethyl zinc, and the complex is high in yield and purity.The diamine-oxazoline zinc complex shows green fluorescence by taking 380nm as an excitation wavelength, the maximum emission wavelength is 491-495nm, and the fluorescence lifetime of the diamine oxazoline zinc complex is 10-10.75 microseconds. Moreover, the diamine oxazoline zinc complex can be directly used for catalyzing the ring-opening polymerization reaction of cyclic lactone and has relatively high catalytic activity, so that a series of high polymer materials with specific structures are obtained.

The invention discloses a carbon quantum dot two-photon fluorescent dye for ultrafast cell staining. The carbon quantum dot two-photon fluorescent dye is prepared by the following steps: 1) preparing a carbon quantum solution by taking m-phenylenediamine as a precursor and adopting a one-step solvothermal method; and 2) separating a green fluorescent component from the carbon quantum prepared in the step 1) by adopting silica gel column chromatography, namely the carbon quantum dot two-photon fluorescent dye. The absolute quantum efficiency of the carbon quantum dot two-photon fluorescent dye for ultrafast cell staining provided by the invention reaches up to 58.65%, and the carbon quantum dot two-photon fluorescent dye shows good biocompatibility and shows an excellent imaging effect even at a low concentration; the fluorescent probe can enter cells within 10 seconds, so that a solid foundation is provided for rapid fluorescence imaging; in addition, the G-CDs can also be used for two-photon imaging, a near-infrared excitation light source is used, and the G-CDs also shows strong green fluorescence and shows excellent imaging performance in 4T1 cells.

The invention relates to a fusarium moniliforme SiC quantum dot fluorescence labeling method and particularly relates to a fluorescence labeling method for fusarium moniliforme SiC in different growth stages by cytotoxicity-free aqueous phase silicon carbide quantum dots, and a long-time-period fluorescence imaging technology for living cells. The method comprises the following steps of preparing a mixed solution of slant strain fusarium moniliforme and a PD culture medium; preparing a mixed culture medium of a mycete liquid-SiC quantum dot aqueous phase solution from the mixed solution and an SiC quantum dot aqueous phase solution at a volume ratio of 3:1; performing constant-temperature shaking culture on the mixed culture medium at a temperature of 28 DEG C and a speed of 200r/min for 3-10 days, thereby obtaining a fusarium moniliforme SiC quantum dot fluorescent mark; applying the mark to seedling root dip dyeing, thereby realizing dynamic monitoring, tracing and fluorescence imaging of a process that the fusarium moniliforme infects plant roots. The method is simple and effective, is low in cost and is capable of realizing fluorescence labeling of the fusarium moniliforme in different periods.

The invention discloses a fluorescent probe for detecting palladium ions, and provides a preparation method and application of the fluorescent probe at the same time. The fluorescent probe is capable of rapidly responding and highly selectively detecting the palladium ions. The fluorescent probe for detecting the palladium ions has a structure as shown in a formula 1 or 2, wherein R is H, CH3 or t-Bu.

The invention discloses a cultivation method of transgenic zebrafish for detecting organic pollutants in a water body. The transgenic zebrafish is prepared by using a promoter for expressing the mosquito catfish cyp1a gene, plasmids for simultaneously expressing green fluorescent protein and luciferase, and body-color-free zebrafish. In the actual water quality monitoring work, technicians in the field can qualitatively evaluate the pollution condition of organic matters in the water body by directly observing the fluorescence of the transgenic zebra fish, and also can quantitatively analyze pollutants in the water body by measuring the activity of luciferase of the transgenic zebra fish.

The invention discloses a method based on spinach green RNA visualization and application thereof, and belongs to the technical field of biology. The invention comprises the application of plant tRNAin protecting and/or stabilizing the interaction between a spinach green RNA aptamer and a fluorescein molecule DFHBI and realizing the visual application of the spinach green RNA in the plant body. The method comprises the following steps: constructing lysine transport RNA-spinach green RNA-lysine transport RNA, subcloning a product into a pEAQHT vector to obtain pEAQHT/KSK, attacking the pEAQHT/KSK into chloroplast-free onion epidermis cells by using a gene gun method, putting onion epidermis on a hypertonic culture medium, infiltrating the onion epidermis into a DFHBI solution, and observing strong enough green fluorescence under a laser confocal microscope. According to the invention, the visualization of RNA in living cells of plants is realized by utilizing methods such as molecularsignal marking, RNA binding marker protein, the RNA-based aptamer and the like.