Fusarium moniliforme SiC quantum dot fluorescence labeling method

A technology of Fusarium moniliformes and fluorescent labeling, applied in chemical instruments and methods, biochemical equipment and methods, luminescent materials, etc., can solve the problems of difficult regulation, cytotoxic structure and size of cadmium-based quantum dots, etc., and achieve easy detection, Simple and uniform distribution of physical and chemical properties on the surface

Inactive Publication Date: 2016-07-20
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] However, the cytotoxicity and structural size of cadmium-based quantum dots are difficult to control, which is the main factor affecting their further application in long-term tracer imaging of living cells.

Method used

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  • Fusarium moniliforme SiC quantum dot fluorescence labeling method
  • Fusarium moniliforme SiC quantum dot fluorescence labeling method
  • Fusarium moniliforme SiC quantum dot fluorescence labeling method

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Embodiment 1

[0031] A silicon carbide quantum dot labeling method for living cell spores of Fusarium moniliforme, the silicon carbide quantum dot aqueous phase solution used is according to the pH value of 6.4±0.2, the silicon carbide quantum dot concentration is 0.03mol / L, and the characteristic excitation wavelength is 340nm, The emission wavelength is 450nm.

[0032] The specific marking steps are:

[0033] A. Scrape 2 rings of the slant strain Fusarium moniliforme preserved in the mold incubator, inoculate it into the prepared PD medium under aseptic conditions for activation, and cultivate it in a constant temperature oscillator (28°C, 200r / min) for 48 Hour.

[0034] B. Operate in an ultra-clean workbench, under aseptic conditions, measure 18ml of PD medium containing Fusarium moniliforme, 6ml of SiC quantum dot aqueous solution, and make a mold liquid-SiC quantum dot aqueous phase with a volume ratio of 3:1 Solution mixed medium;

[0035] C. Place the mixed medium in a constant te...

Embodiment 2

[0039] A silicon carbide quantum dot labeling method for living cells of Fusarium moniliforme, the pH value of the silicon carbide quantum dot aqueous phase solution used is 6.4±0.2, the concentration of silicon carbide quantum dots is 0.03mol / L, the characteristic excitation wavelength is 340nm, and the emission wavelength 450nm.

[0040] The specific marking steps are:

[0041] A. Scrape 2 rings of the slant strains stored in the mold incubator and inoculate them into the prepared PD medium (components: potato and glucose) under aseptic conditions for activation. Constant temperature oscillator (28°C, 200r / min) for 48 hours.

[0042] B. Operate in an ultra-clean workbench, under aseptic conditions, measure 18ml of PD medium containing Fusarium moniliforme, 6ml of SiC quantum dot aqueous solution, and make a mold liquid-SiC quantum dot aqueous phase with a volume ratio of 3:1 Solution mixed medium;

[0043] C. Place the mixed medium in a constant temperature shaker (28°C,...

Embodiment 3

[0047] A silicon carbide quantum dot labeling method for living cells of Fusarium moniliforme mycelia, the pH value of the silicon carbide quantum dot aqueous phase solution used is 6.4±0.2, the concentration of silicon carbide quantum dots is 0.03mol / L, and the characteristic excitation wavelength is 340nm. The emission wavelength is 450nm.

[0048] The specific marking steps are:

[0049] A. Scrape 2 loops of the slant strains stored in the mold incubator, inoculate them into the prepared PD medium under aseptic conditions for activation, and cultivate them in a constant temperature oscillator (28°C, 200r / min) for 48 hours.

[0050] B. Operate in an ultra-clean workbench, under aseptic conditions, measure 18ml of PD medium containing Fusarium moniliforme, 6ml of SiC quantum dot aqueous solution, and make a mold liquid-SiC quantum dot aqueous phase with a volume ratio of 3:1 Solution mixed medium;

[0051] C. Place the mixed medium in a constant temperature shaker (28°C, 20...

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Abstract

The invention relates to a fusarium moniliforme SiC quantum dot fluorescence labeling method and particularly relates to a fluorescence labeling method for fusarium moniliforme SiC in different growth stages by cytotoxicity-free aqueous phase silicon carbide quantum dots, and a long-time-period fluorescence imaging technology for living cells. The method comprises the following steps of preparing a mixed solution of slant strain fusarium moniliforme and a PD culture medium; preparing a mixed culture medium of a mycete liquid-SiC quantum dot aqueous phase solution from the mixed solution and an SiC quantum dot aqueous phase solution at a volume ratio of 3:1; performing constant-temperature shaking culture on the mixed culture medium at a temperature of 28 DEG C and a speed of 200r/min for 3-10 days, thereby obtaining a fusarium moniliforme SiC quantum dot fluorescent mark; applying the mark to seedling root dip dyeing, thereby realizing dynamic monitoring, tracing and fluorescence imaging of a process that the fusarium moniliforme infects plant roots. The method is simple and effective, is low in cost and is capable of realizing fluorescence labeling of the fusarium moniliforme in different periods.

Description

technical field [0001] The invention relates to a fluorescent labeling method of SiC quantum dots of Fusarium moniliforme, which belongs to the technical field of biological application of quantum dots. Background technique [0002] Fusarium is a worldwide distribution of fungi, it can not only survive winter and summer in the soil, but also infect a variety of plants (food crops, economic crops, medicinal plants and ornamental plants), causing plant root rot, stem There are more than 100 species of host plants, infecting the vascular system of host plants, destroying the vascular bundles of plant transport tissues, and producing toxin hazards in the process of growth, development and metabolism Crops, causing crop wilting and death, is one of the most difficult important diseases in production. There are many common toxin-producing molds of Fusarium, and Fusarium moniliforme is one of them. In addition to causing root rot and stem base rot, it also causes symptoms such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C09K11/65
CPCC09K11/65C12Q1/04
Inventor 宋月鹏王征柳洪洁高东升朱彦敏孙丰飞尹承苗康杰
Owner SHANDONG AGRICULTURAL UNIVERSITY
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