61 results about "Wilting" patented technology

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The invention relates to the technical field of agricultural intelligent monitoring and regulation and control, and discloses a rice three-dimensional seedling raising seedling growth monitoring, diagnosis and regulation and control method, which comprises the following steps: S1, when any hanging basket reaches a target position, reducing the movement speed of the hanging basket, and obtaining a serial number image of the hanging basket; s2, when the hanging baskets stop moving, sequentially acquiring images right above and obliquely above all seedling trays in the hanging baskets corresponding to the serial number images of the hanging baskets; s3, respectively obtaining the areas of a green plant area, a yellow plant area, a black soil area and a seedling block area in the images right above and obliquely above the seedling tray, calculating the pixel number of each area, and obtaining the pixel ratio of different areas; and S4, acquiring the coverage rate, health index and spindling index of each seedling tray according to the ratio of pixels in different areas, dividing the seedlings in the seedling trays into five types of normal, spindling, vertical withering, insufficient fertilizer and strong, obtaining a growth vigor diagnosis result of the seedlings in the seedling trays, and generating a seedling growth vigor regulation and control prescription according to the growth vigor diagnosis result.

The invention discloses application of a tea tree CsNAC25 gene in negative regulation of cold resistance of plants, the gene belongs to a tea tree NAC transcription factor family, and the nucleotide sequence of the gene is shown as SEQ ID NO.1. The tea tree CsNAC25 gene negatively regulates the cold resistance of the tea tree, and the gene expression is reduced under low temperature induction. The over-expression of the gene in tobacco weakens the cold resistance of a transgenic plant, and the expression of CsNAC25 is silenced by adopting a VIGS technology in a tea tree, so that the cold resistance of the tea tree is remarkably improved. Silencing CsNAC25 effectively reduces the wilting degree and the ion leakage rate of tea seedling leaves under low temperature stress, activates a key CBF signal channel and promotes accumulation of osmoregulation substances. The application of the application is expected to effectively improve the cold resistance when low-temperature disasters such as late spring coldness occur, and has important theoretical significance and wide application prospects for guaranteeing the stable yield and quality of the tea industry.

The application discloses a protein kinase gene positively regulating drought resistance of oats AVESA.00022b.r1.6C0001455 and application thereof, and belongs to the technical field of plant genetic engineering. AVESA.00022b.r1.6C0001455 The CDS sequence of the gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. Expression mode analysis shows that the gene is significantly induced and up-regulated under drought stress; virus-induced gene silencing verification shows that silencing the gene can significantly reduce the drought resistance of the oats; overexpression verification shows that overexpression of the gene can significantly enhance the drought resistance of the oats, and the performance is that wilting is delayed, the water loss rate is reduced, and the survival rate is improved. The application first proves that the gene is a key positive regulation factor of drought resistance in the oats, and can be used for cultivating drought-resistant transgenic plants and as a drought-resistant molecular marker for assisted breeding, and has important application value in genetic improvement of crop drought resistance.

This application relates to the technical field of tomato genetic breeding, and specifically to molecular markers associated with low-temperature tolerance in tomato seedlings and their applications. This molecular marker comprises an Indel deletion sequence from positions 6494531 to 6494551 on chromosome 5 of the tomato genome, SL2.50ITAG2.4. This molecular marker enables accurate detection of low-temperature tolerance in tomato seedlings. Furthermore, this application provides closely associated molecular markers for cloning genes that control the degree of leaf wilting caused by water loss in tomato seedlings under low-temperature stress, and for breeding tomato varieties for low-temperature tolerance, thus possessing significant application value.

The invention relates to the technical field of tea processing, and particularly discloses a preparation technology of West Lake Longjing tea. The product comprises West Lake Longjing green leaves which are subjected to multi-layer temperature-control humidity-control tedding withering, vibration grading screening and independent constant-temperature conveying; the preparation method comprises the following steps: firstly tedding fresh leaves, then grading through a vibrating screen classifier, and then entering an independent fixation section; standing for moisture regaining, and uniformly reducing the temperature of the leaves to 30-35 DEG C; then performing classification stir-frying and shaping, and controlling the temperature and the duration to be 45-60 minutes; refining and screening to remove powder and foreign matters; and finally, semi-finished products are subjected to nitrogen-filled fresh-keeping The composition can be used for high-end green tea consumption, gift tea and exported high-quality tea, and has the advantages that the powder rate is smaller than or equal to 2%, the aroma retention rate is larger than or equal to 92%, the sensory evenness is excellent, the soup color is light green and bright, and the taste is fresh, mellow, sweet and refreshing; in addition, the preparation method has the advantages that the process chain is continuous, parameters are accurate, quality is stable, energy consumption is reduced by 15%, and production efficiency is improved by 20%.

The invention relates to the field of plant biotechnology and molecular biology, in particular to application of a VvECT10 gene in regulation and control of drought resistance of grapes. A strain with overexpressed VvECT10 gene is slow in leaf water loss and light in withering degree under a drought condition, the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2) is remarkably reduced, and the activity of peroxidase (POD) and catalase (CAT) is remarkably improved, which indicates that the overexpression of the VvECT10 gene can enhance the drought resistance of the grape plant and relieve oxidative damage.

The application belongs to the field of fresh flower preservation and relates to a SSS polypeptide, a preservative and application. grf6 and grf8 The mutant has the phenotype of early leaf senescence. A small piece of amino acid of the JID region of the MYC2 protein is prepared into a polypeptide powder, dissolved in ultrapure water to prepare a polypeptide water agent, and sprayed on the surface of the flower. The research of the application shows that spraying the polypeptide reagent can prolong the flowering period of fresh flowers, delay senescence, slow down the drooping of flowers, and slow down the degree of wilting of flowers, thereby achieving the effect of fresh flower preservation. The polypeptide fresh flower preservative has the advantages of safety, non-toxicity, simple operation and obvious effect, and has good application prospect.

This invention provides a leaf moisturizing device for indoor aphid experiments, comprising: a cup body and a cup lid. The cup lid covers the top of the cup body, and a cup lid hole is provided at the center of the top of the cup lid. A water storage tube protruding upwards is installed on the cup lid hole. A high-density sponge is installed at the bottom opening of the water storage tube. The plant leaf stem or the entire plant stem is placed inside the high-density sponge, and the plant leaf stem or the entire plant stem extends downwards into the cup body. Nutrient solution is loaded in the water storage tube above the high-density sponge. This leaf moisturizing device for indoor aphid experiments has a simple structure and is easy to install and use. It can solve the problem of insufficient leaf moisturizing time and leaf wilting caused by ordinary leaf disc methods. It can also solve the problem of aphids dying after escaping and coming into contact with the liquid, thus affecting the statistics. It is especially suitable for the early effect exploration and coarse screening of large quantities of raw materials and agents, and for situations requiring a large number of treatment groups.

The application provides a gene and an application thereof CaCA2 The application belongs to the technical field of gene breeding and relates to application of a gene in pepper resistance to low-temperature and weak-light stress. CaCA2 The application discloses a gene and an application thereof CaCA2 The transient overexpression of the gene can slow down wilting of pepper plants, improve net photosynthetic rate of pepper leaves, expression amount of antioxidant enzyme genes, soluble sugar, free proline and mineral element content, and simultaneously reduce intercellular CO2 concentration and malondialdehyde content. CaCA2 After the VIGS technology is used to transiently silence the gene, the plant height, stem diameter, biomass and root morphological parameters of the pepper are reduced in varying degrees, and the gene causes stoma to close, and the overall fluorescence intensity, photosynthetic performance index and trace element content are reduced. CaCA2 The transient overexpression of the gene in the pepper can relieve wilting of the pepper plants and improve the resistance to low-temperature and weak-light stress, and the resistance is weakened after the gene is silenced.

The invention belongs to the technical field of plant planting, and provides a Hainan camellia oleifera high-survival-rate drought-resistant afforestation and biological nutrient solution preparation method. The special nutrient solution for the camellia oleifera comprises the following raw materials in parts by weight: 6-12 parts of a probiotic agent, 8-12 parts of potassium fulvate, 15-20 parts of potassium nitrate, 60-70 parts of a coco coir extract and 95-105 parts of water. Through the synergistic effect of the afforestation method and the special nutrient solution for camellia oleifera, the survival rate and preservation rate of camellia oleifera afforestation are remarkably increased. Wherein the special nutrient solution has high-temperature-resistant, drought-resistant and root-promoting effects, and can effectively promote the growth of roots of camellia oleifera, improve the water-retaining capacity of leaves and increase the content of available phosphorus in soil, thereby enhancing the stress resistance and growth vigor of plants; in the drought period, the leaf wilting degree of the oil-tea camellia adopting the drought-resistant afforestation method is far lower than that of a traditional method, the water content of soil is higher, and stage drought stress can be effectively resisted.

A concrete retaining wall capable of vegetation according to the present invention may include: a vegetation hole formed to penetrate from the front to the rear of the concrete retaining wall to form a vegetation space; a vegetation protection tube formed in a tubular shape and inserted into the vegetation hole; and a water inflow member that penetrates the vegetation hole and is inserted into the soil located on the rear of the concrete retaining wall.

To accurately calculate a wilting feature quantity for a plant.SOLUTION: An analysis system includes at least one processor. The at least one processor acquires a first image capturing a target plant including one or more leaves, performs segmentation on the first image to detect the one or more leaves in the first image, specifies, among the detected one or more leaves, a leaf located in the foreground as a target leaf, and sets a plurality of tracking points on the target leaf. The processor further acquires a plurality of second images capturing the target plant, detects positions of the tracking points in each of the second images, calculates transitions of the tracking points, and calculates a wilting feature quantity indicating features related to the wilting of the target plant based on the transitions of the tracking points.SELECTED DRAWING: Figure 1

The invention provides a method for preventing and treating waxberry wilting disease. The method comprises the following steps: spraying a bamboo vinegar stock solution diluted by 5-10 times to soil, spraying a mixed solution of 1600-2000 times of thifluzamide and 900-1200 times of difenoconazole to tree bodies, and realizing disease prevention and treatment in cooperation with specific spraying periods, astragalus sinicus planting and nitrogen fertilizer control. Wherein the soil is sprayed for one time within 5 days after fruit picking and for one time within 2.5-3.5 cm of summer shoots, the tree body is sprayed for one time within 7 days after fruit picking and for one time within 4-6 cm of summer shoots, and the spraying period of the same batch of tree bodies is 2 days or more later than that of the soil; astragalus sinicus is sown in October, the nitrogen fertilizer application amount is controlled throughout the year, the fruit / compound fertilizer weight ratio is greater than 50, and no urea is applied. The method for preventing and treating the waxberry wilt disease only twice a week, the dosage is remarkably reduced, the immunity of tree bodies for resisting the wilt disease is further enhanced by improving the soil environment, improving the root activity, controlling the application amount of the nitrogen fertilizer and keeping the moderate tree vigor of waxberries, the prevention and treatment effect reaches 69% or above, and the method has the advantages of being environmentally friendly, efficient, easy and convenient to operate and suitable for large-scale application and popularization.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

The application relates to the biotechnology field and discloses a drought stress related alfalfa gene and application thereof, and the MsCAS15 gene is cloned from alfalfa, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the encoded dehydrin is shown as SEQ ID NO. 2. The protein is rich in glycine and lysine and has the functions of protecting cell membranes and biological macromolecules. The application obtains a transgenic plant overexpressing the MsCAS15 by constructing a recombinant expression vector containing the gene and using an agrobacterium-mediated method. The experimental results show that overexpression of the gene enhances the drought resistance of the plant, and the specific performance is that the seed germination rate of the transgenic plant is higher than that of the wild type under osmotic stress, and under the natural drought condition of soil, the wilting degree of the transgenic plant leaves is reduced, and the growth state is better than that of the wild type. The application provides an effective gene resource for cultivating new drought-resistant crop varieties.

The application discloses a winter melon seedling salt tolerance phenotype grading method based on a deep visual perception network, and belongs to the technical field of cross of wisdom agriculture and computer vision. The technical scheme is as follows: a winter melon seedling image dataset containing four salt tolerance grades is constructed; a deep visual perception network GYSalt-Net is constructed and trained, the network simultaneously captures macro wilting and micro lesion features by integrating a global-local hybrid feature extraction module, introduces a partial convolution adaptive context enhancement module to suppress complex background noise and enhance leaf region features, and adopts a dynamic up-sampling module based on point sampling to realize lightweight of the model and high-fidelity reconstruction of features; and the trained network is used to infer the salt tolerance grade of the seedling image. The application realizes high-throughput, automatic and accurate grading of the winter melon seedling salt tolerance, and the model is lightweight and suitable for deployment of portable computing devices.

An additive for preservation of cell, tissue and / or food useful for, among other applications, increasing the shelf life of foods and providing greater food safety, and the use of a synthetic peptide for preparing the additive. The additive provides substantial fruit preservation upon a single application, providing for the treatment of plants or food to prevent the occurrence of pests such as bacteria or fungi, such as, for example, those related to fruit rot. The additive provides substantial preservation of flowers or inflorescences upon a single application, providing an alternative against rapid wilting, rot or fungal growth associated with the storage, transport and / or medicinal processing of flowers or inflorescences of phytochemical interest. The additive provides substantial preservation of the metabolic state of sprouts or seedlings, and slows down the seed germination process, thus being useful for synchronizing germination, adjusting time of planting or germination, among other agronomic or botanical advantages.

This invention belongs to the field of plant genetic engineering technology, specifically relating to loquat. EjHB7 This invention obtains genes, proteins, and their applications in regulating plant drought tolerance through genetic transformation technology. EjHB7 Transgenic Arabidopsis plants, under drought stress, showed less leaf wilting than wild-type plants. EjHB7 Reducing reactive oxygen species (H2O2 and O2) by increasing the activity of POD and SOD enzymes. ‑ The accumulation of these nutrients improves the drought resistance of transgenic Arabidopsis thaliana. Additionally, EjHB7 The reduced drought tolerance of the silenced transgenic loquat plants indicates that... EjHB7 It plays an important role in regulating plant drought resistance.

The application discloses a kind of yeast black tea products and process method for preparing by double biological enzyme fermentation, which includes the process steps of picking and removing impurities of fresh leaves, light heat wilting, light heat steam biological fermentation, enzyme oxygenation kneading, deagglomeration double enzyme biological fermentation, hot air aroma extraction initial baking and vacuum freeze drying etc.The yeast black tea with higher fermentation enzymolysis power can be prepared by process optimization, which has strip shape, red leaves, sweet aroma, the activity of tea polyphenol oxidase (ppo) is kept at 1.5-2 U / G, and can drive other plant leaves to carry out mixed fermentation enzymolysis reaction, to make high-quality composite black tea, which has wide application prospect in the field of fermentation composite black tea.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of TaSIZ1 protein, TaSIZ1 gene and related biological materials in improvement of drought resistance of plants. The wheat TaSIZ1 gene is knocked out by using a gene editing technology, two homozygous knockout strains Taiz1-6 # and Taiz1-13 # are obtained through screening, and compared with a wild type, the TaSIZ1 gene knockout mutant has the advantages that the fresh weights of the overground parts are obviously higher and the plant wilting degree is obviously lower under the drought treatment condition; after drought stress, the plant is subjected to rehydration treatment, the survival rate of the TaSIZ1 gene knockout mutant is remarkably improved, the drought resistance of wheat can be remarkably enhanced, and a feasible method is provided for cultivating stress-resistant wheat.

The application discloses a water supply and discharge control method and device applied to algae cultivation, and the method comprises the following steps: collecting a current water quantity parameter of a purification device during cultivation of algae organisms in the purification device; determining whether a target control operation needs to be performed on the purification device according to the current water quantity parameter; if yes, generating a target water quantity control parameter according to the current water quantity parameter, and performing the target control operation on the purification device according to the target water quantity control parameter. It can be seen that the algae organisms in the purification device can be intelligently supplied with water and discharged according to the application, the problem of low water quantity control accuracy in the traditional time-based water supply and discharge method is solved, the wilting and crazy reproduction of the algae organisms are reduced, the normal growth of the algae organisms is ensured, and the air purification work of the purification device can be normally performed.

The application belongs to the technical field of cotton germplasm waterlogging tolerance, and specifically provides a method for rapidly identifying the waterlogging tolerance of cotton germplasm. The method provided by the application comprises the following steps: taking Zhongmian 9101 as a waterlogging-sensitive control and Zhongmian 9001 as a waterlogging-tolerant control, using indoor double-pot simulation waterlogging technology to perform waterlogging treatment on four-leaf expected evaluation materials, then removing the waterlogging and performing light treatment, and observing the wilting degree of the leaves of different cotton materials. The application establishes a rapid and efficient indoor method for identifying the waterlogging tolerance of cotton materials, overcomes the shortcomings of the prior art, such as a long period, high cost, and being easily affected by external factors such as environment, and the like. The method is fast in identification speed, accurate and intuitive in effect, strong in controllability, and can batch-screen waterlogging-tolerant cotton germplasm resources or breeding materials.

The utility model discloses a kind of wilting devices for green tea fresh leaves, it is related to green tea processing technical field, including wilting box, the wilting box two sides are respectively provided with air inlet and air outlet, the wilting box side wall is also hinged with box door, the wilting box inside is respectively provided with and is equipped with fixed rod, the fixed rod is slidably sleeved on the fixed rod, and the fixed rod surface is located in the position of the lower end of fixed ear also sleeved with first spring, the placing box is equipped with rack, the wilting box is also equipped with driving component for driving the vibration of placing box. In the utility model, through the driving component and handle and second spring being set, tea leaf can be driven to vibrate up and down and horizontally during wilting process, so that tea leaf is rolled and scattered, tea leaf is prevented from accumulating, and the wilting efficiency of tea leaf is improved.

The invention belongs to the technical field of food processing and health-care tea preparation, and particularly discloses lithocarpus litseifolius and folium cortex eucommiae composite health-care tea and a preparation method thereof. The product is prepared by compounding fresh Lithocarpus litseifolius leaves and fresh Eucommia ulmoides leaves as raw materials according to a weight ratio of (6-8): (2-4). The preparation method comprises the following steps: synergic withering, steam-microwave combined fixation, pre-dehydration and spreading for cooling, anti-sticking synergic rolling, optional coupling fermentation, segmented drying, screening, sterilization and packaging. Wherein the enzyme activity can be efficiently passivated, and color fixation and fragrance preservation can be realized through steam-microwave combined enzyme deactivation; during rolling, a food-grade physical separant is added, and stage pressure control of air compression, light compression, medium compression and air compression is combined, so that the problem of adhesion of the leaves is effectively solved, and flavor fusion and strip tightening are promoted. According to the method, the retention rate of chlorogenic acid, flavone and other active ingredients in the product is remarkably increased, the taste and appearance integrity of tea soup are improved, and the method is suitable for large-scale production and has a good market application prospect.

The application discloses a kind of intelligent irrigation methods and systems of traditional Chinese medicinal material seedlings based on reinforcement learning, comprising: obtaining the state perception parameter of current seedling cultivation environment, including soil moisture stress degree, seedling growth stage index and canopy wilting representation value;Based on state perception parameter and cumulative water stress degree, obtain the effective component accumulation estimate value of current seedling;State perception parameter and effective component accumulation estimate value are input into pre-trained irrigation decision model, and the irrigation action decision including irrigation duration and irrigation intensity is obtained;After executing irrigation, obtain updated state perception parameter and updated cumulative water stress degree, and calculate updated effective component accumulation value;Reward value is calculated based on the change of state before and after updating, and the irrigation decision model is updated online using reward value.The present application solves the technical problem that the prior art cannot dynamically balance seedling survival guarantee and effective component improvement.

The invention discloses application of an MsGDSL gene in improvement of salt tolerance, and belongs to the technical field of biology. According to the invention, MsGDSL is positioned in a cell nucleus and cytoplasm through subcellular localization. Yeast self-activation detection finds that the gene has no transcriptional self-activation activity. Through construction of an overexpression vector and a silence vector, it is found that after the MsGDSL gene is overexpressed, the expression quantity of MsGDSL is remarkably increased, the root length of a transgenic line is remarkably higher than that of WT, the withering and yellowing degree is lower than that of WT, the chlorophyll content is higher than that of WT, the conductivity and the death rate are lower than that of WT, and the salt tolerance function is enhanced; and after the MsGDSL gene is silenced, the expression quantity of the MsGDSL is reduced, the chlorophyll content and survival rate of the transgenic line are lower than those of the transgenic line dyed by CK, DAB and NBT, the color is darker, and the oxidative damage is serious. The positive regulation effect of the MsGDSL gene in the plant salt tolerance is found for the first time, and the MsGDSL gene has great significance in the field of cultivation of new varieties (lines) of salt-tolerant plants.

The application discloses ZmPHR1 protein and application of a coding gene thereof in regulating drought resistance of plants. The application finds that the leaf water content of a ZmPHR1 overexpression corn strain under drought conditions is significantly higher than that of a wild type, and the leaf wilting degree, transpiration rate, stomatal conductance and stomatal opening degree are all significantly reduced, and the transpiration rate, stomatal conductance and stomatal opening degree of a zmphr1 mutant are all significantly higher than those of the wild type, by detecting the drought resistance of the ZmPHR1 overexpression corn strain and the zmphr1 mutant. The application finds that the ZmPHR1 protein of corn can positively regulate the drought resistance of plants for the first time, and the drought resistance of plants can be effectively improved by increasing the expression amount of the ZmPHR1 gene. The finding of the drought resistance function of ZmPHR1 provides a new gene target and resource for cultivating new drought-resistant plant varieties, and also provides a theoretical basis for elucidating the molecular mechanism of plant drought stress signal response.

The application belongs to the technical field of plant genetic engineering, and particularly relates to TaSIZ1 protein, TaSIZ1 gene and application of related biological materials of the TaSIZ1 gene in improving drought resistance of plants. The application uses a gene editing technology to knock out a wheat TaSIZ1 gene, and two homozygous knockout strains Tasiz1-6 # and Tasiz1-13 # are screened. Compared with a wild type, the TaSIZ1 gene knockout mutant has a significantly higher fresh weight of an aboveground part and a significantly lower wilting degree under a drought treatment condition; after a rehydration treatment is performed on the plant after the drought stress, the survival rate of the TaSIZ1 gene knockout mutant is significantly improved, the drought resistance of the wheat can be significantly enhanced, and a feasible method for cultivating stress-resistant wheat is provided.

The application belongs to the field of plant growth and relates to a gene for regulating flowering and disease resistance of plants, in particular to a gene for regulating flowering, a recombinant vector and application. In the research process of disease resistance breeding, a NBS-LRR type gene of Nicotiana benthamiana is cloned by the research group NbLOV1 . Bioinformatics analysis results show that the gene is closely related to the response of plants to fungal pathogens, and in the experiment of verifying the gene function by gene editing knockout, the plants created by using the knockout vector for NbLOV1 and the Agrobacterium-mediated transformation method have obvious differences compared with the control: after knocking out NbLOV1 , the budding and flowering time of Nicotiana benthamiana is obviously earlier than that of the control. The mutant plant with the knockout NbLOV1 gene presents strong resistance to fusarium, only with slightly yellow leaf color and slight wilting; while the tobacco of the control presents damaged vascular bundle, seriously wilting leaf surface and necrotic spots.