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739 results about "Plant variety" patented technology
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Plant variety may refer to Variety (botany), a taxonomic nomenclature rank in botany, below subspecies, but above subvariety and form Plant variety (law), a non-taxonomic, exclusively legal term An informal and ambiguous substitute for form (botany) (a taxonomic nomenclature rank in botany, below variety (botany))
Plant breeding method
InactiveUS20050144664A1Other foreign material introduction processesAgricultureNumeral systemPlant variety
Methods for using genetic marker genotype (e.g., gene sequence diversity information) to improve the process of developing plant varieties (e.g., single cross hybrids) with improved phenotypic performance are provided. Methods for predicting the value of a phenotypic trait in a plant are provided. The methods use genotypic, phenotypic, and optionally family relationship information for a first plant population to identify an association between at least one genetic marker and the phenotypic trait, and then use the association to predict the value of the phenotypic trait in one or more members of a second, target population of known marker genotype. Methods for identifying new allelic variants affecting the trait are also provided. Plants selected, provided, or produced by any of the methods herein, transgenic plants created by any of the methods herein, and digital systems for performing the methods herein are also provided.
Owner:EI DU PONT DE NEMOURS & CO +1
Agricultural management using biological signals
InactiveUS20120123817A1Maximizes desired crop outcomeData processing applicationsEnvironmental resource managementAgricultural engineering
A method and system for customizing agriculture management using biological signals and making decisions according to pre-programmed algorithms, including decisions related to irrigation, prediction of future yield, characterization of plant varieties, and administration of topical applications.
Owner:SMARTFIELD
Hormone-free tissue culture method for dendrobium candidum
InactiveCN102613086ALow costReduce waste of resourcesPlant tissue cultureHorticulture methodsGenetic diversityPlant variety
The invention discloses a hormone-free tissue culture method for dendrobium candidum, which comprises four tissue culture stages including germinating of seeds of the dendrobium candidum into spherical stems, multiplication and differentiation, and rootage and sound seedlings at stem sections; culture media used at the four stages include a hormone-free MS (Murashige and Skoog) culture medium, a hormone-free MS basal culture medium plus 15-20 g / l mashed potato culture medium, a hormone-free MS basal culture medium plus 15-20 g / l mashed banana plus 15-20 g / l mashed potato culture medium and a hormone-free 1 / 2 MS basal culture medium plus 15-20 g / l mashed banana plus 15-20 g / l mashed potato culture medium. The polysaccharide content and total alkaloid content in the culture of the dendrobium candidum obtained by hormone-free culture are increased compared with hormone-containing culture; the growth state of the dendrobium candidum is normal; the genetic diversity of the plant variety is protected; with the adoption of the method, the tissue culture of the dendrobium candidum is more convenient, green and environmentally friendly, and the resources are saved.
Owner:NANJING AGRICULTURAL UNIVERSITY
Device for three-dimensional coupling and purification of water quality by lifting plant sinker and floater
InactiveCN101830565AMeet the needs of growthPromote recoveryWater resource protectionSustainable biological treatmentAquatic ecosystemWater quality
Owner:HOHAI UNIV
Method for recognizing image of plant leaf
InactiveCN101916382AIncrease credibilityImprove recognition rateCharacter and pattern recognitionNeural learning methodsGraphicsFeature extraction
The invention discloses a method for recognizing an image of a plant leaf. The method comprises a training stage and a recognition stage. The training stage comprises the following steps of: inputting an image training set of the plant leaf; and storing a training result after performing batch preprocessing, batch feature extraction and neural network classifier training by using a system. The recognition stage comprises the following steps of: allowing a user to input a single image of the plant leaf of which the variety is unknown to the system and mark the leaf base point coordinate and leaf apex point coordinate of the leaf; performing preprocessing and feature extraction on the single image by using the system; and classifying by using the neural network classifier, outputting a plant variety list and attaching detailed graphics and text information to each item of the list. The method has the advantages of outputting a plurality of high-accuracy candidate results, greatly enhancing recognition credibility, supporting a scalar quantity of leaf features as well as leaf features in complex forms (such as a matrix form) and increasing the recognition rate of the plant leaf.
Owner:GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
Solid cultivation system and mounting method thereof
InactiveCN101473762AEfficient use ofEasy to implementClimate change adaptationGreenhouse cultivationGreeningPlant variety
The invention discloses a spatial culture system and a mounting method thereof. The spatial culture system is used for spatial culture of plants, and comprises a frame and a substrate; the substrate is arranged on the frame for planting plants; and the frame is used for fixing the substrate. The method adopts the spatial culture system which can help plant the plants on a stereo frame, perform large-scale indoor and outdoor stereo surface greening, conveniently replace plant varieties and overall design, effectively improve the space utilization rate, improve the delight of life, and is convenient in implementation and realizing industrialized greening.
Owner:深圳市润和天泽环境科技发展股份有限公司
System for planning the planting and growing of plants
A method for generating a plan facilitating users to plant, grow, harvest and maintain selected plant varieties includes receiving, via at least one processor, user input corresponding to at least one of: the selected plant varieties, desired servings of the selected plant varieties, geographic information for the user and available gardening hardware. The method includes determining, via the at least one processor, quantities of the selected plant varieties to plant, a location layout for planting each of the selected plant varieties and plant care tasks associated with each of the selected plant varieties. The method also includes graphically presenting, via the at least one processor, the location layout and the plant care tasks associated with each of the selected plant varieties to the user.
Owner:LYNDS HEATHER
Method for recovering vegetation on strong-acid tailing waste land without soil
ActiveCN104624620AGood water solubilityEffective penetrationContaminated soil reclamationHorticultureRevegetationAcid water
The invention discloses a method for recovering vegetation on a strong-acid tailing waste land without soil. The method is characterized by comprising the following steps: A. performing early-stage preparations, and draining off water; B. changing the pH value of the land; C. culturing the fertility of the tailing waste land; D. screening and culturing plant varieties; and F. managing transplanted seedlings. The method has the advantages that by adopting the method for recovering the vegetation without soil, disturbance to the tailing land is reduced, and the purposes that water can be drained off, the influence of acid water to the vegetation can be alleviated, the heavy metals can be stabilized, and the living environment of plants is improved are achieved. In addition, according to the method, the cost is low, the effect taking is rapid, the effect is good and the operability is high.
Owner:CHINA NERIN ENG
Plant stress tolerance correlative protein, coding gene and application thereof
The invention discloses a plant stress tolerance correlative protein, a coding gene and an application thereof. The plant stress tolerance correlative protein is the protein of the following (a) or (b): (a), a protein formed by an amino acid sequence shown in a sequence 1 in a sequence table; (b), a protein which is formed in a way that the amino acid sequence of the sequence 1 in the sequence table is substituted and / or lost and / or added by one or a plurality of amino acid residues, is correlative to plant stress tolerance and is derived from the sequence 1. The invention also provides the coding gene of the protein. The coding gene of the plant stress tolerance correlative protein is introduced into plant cells, and a transgenic plant variety with reinforced adverse circumstance stress tolerance to abiotic substances, such as salt, and the like can be obtained. The invention has very important theoretical and practical meanings for improving and reinforcing the stress tolerance of plants, increasing the yield, quickening the breeding process of stress tolerance molecules and effectively saving water resources.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Method for remedying soil polluted with cadmium by using interplanting
ActiveCN102144467AReduce contentTo achieve the purpose of food safetyHorticultureSoil propertiesWater source
The invention provides a method for remedying soil polluted with cadmium by using interplanting. In the soil polluted with cadmium, cadmium high-accumulation plants and cadmium low-accumulation plants are planted simultaneously in an interplanting manner and planted alternatively at the number ratio of 1 to 1; four plants around each plant are all different from the plant in variety, the plant spacing is 10 to 30 cm and the line spacing is 10 to 30 cm; the plants are watered by using a water source without being polluted by heavy metal and the field moisture capacity of the soil is kept to be 80%; compound fertilizers and carbamide are applied, depending on the soil property and the crop variety; edible parts of the interplanted plants are harvested at the harvest time and the cadmium content in the plant variety with relative low cadmium accumulation is decreased to the specified safe standards for consumption in China, so that the purpose of yielding safe edible products from the soil polluted with cadmium is achieved. The method for remedying soil polluted with cadmium by using interplanting, provided by the invention, has the advantages of rational design, low expense and strong operability and can be applied to the purpose of yielding products at the same time of remedying the soil polluted cadmium.
Owner:CHINA JILIANG UNIV
Plant configuration and ecological engineering technology for embankment
InactiveCN1526886AIncreased shear strengthWon't wash awayExcavationsHorticultureStone wallEngineering
The present invention relates to the embankment protection in water conservancy and hydroelectric power system, especially inner slope in water reservoir. The plant configuration and ecological engineering technology of the present invention is superior to traditional concrete, laid stone wall, anchor pile and other traditional rigid means, which are lack of ecological effect of purifying water and lasting effect, and superior to available biological engineering means with non ideal effect. The present invention is one ecological engineering technology with vetiver as main body and other plant variety, and has superior ecological effect, lasting slope protection effect, low cost and simple construction process.
Owner:SOUTH CHINA PLANT INST CHINESE ACAD OF SCI +1
Three cotton ABF/AREB/ABI5/DPBF type transcription factors and coding genes and application thereof
InactiveCN102060919AMicrobiological testing/measurementMicroorganism based processesBiotechnologyNucleotide
The invention discloses three novel cotton ABF / AREB / ABI5 / DPBF type transcription factors GhABF2, GhABF3 and GhABI5, and coding genes and application thereof. Amino acid sequences of the GhABF2, GhABF3 and GhABI5 are shown as SEQ ID NO: 2, 5 and 8 respectively. Nucleotide sequences of the coding genes GhABF2, GhABF3 and GhABI5 are shown as SEQ ID NO: 1, 4 and 7 respectively. The invention also discloses the structural characteristics on genomic deoxyribonucleic acid (DNA) and a ribonucleic acid (RNA) editing law of reading frame regions of the coding genes GhABF2, GhABF3 and GhABI5. The invention also discloses the cotton expression characteristics and yeast expression characteristics of the three genes and the characteristic that the three genes can improve the drought resistance of arabidopsis thaliana. The genes provide gene resources for culturing a plant variety resistant to abiotic stress, and have great significance for improving the abiotic stress resistance of plants.
Owner:THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI +1
Method for propagating tea plants
InactiveCN101569266AImprove survival rateAvoid the influence of natural environmental factorsAgriculture gas emission reductionCultivating equipmentsGrowth hormonePlanting seed
The invention relates to a method of propagation, in particular to a method for propagating tea plants (also known as camellia sinensis). The method comprises the following steps: (1) the first stage, inducing primary roots: cutting the collected branches of the tea plants into 3cm to 5cm long sprigs and carrying out the root induction for 10 to 30 minutes by growth hormones, then, cutting the processed sprigs in a soilless culture substrate and further arranging the substrate in a rooting environment under artificial regulation; and (2) the second stage, inducing secondary roots: planting the whole tea plant seed which is subjected to the first stage of induction of the primary roots in a nutrition bag filled with nutrition soil, and watering the tea plant seed for 15 to 25 days by depending on whether the surface of the nutrition soil turns white. By carrying out the root induction of the two stages, the invention has the advantages of simple operation, high propagation coefficient, low investment cost and easy popularization; and the invention constitutes the best way to propagate the rare difficult-to-root tea plant variety.
Owner:LISHUI AGRI SCI
Methods for modifying plant responses to stress and correspondingly derived plants
InactiveUS7615681B2Improve plant resistanceImprove the immunityHydrolasesImmunoglobulinsGrowth plantStress conditions
Plant stresses such as pest drought, and excessive temperatures can lead to significant losses of crops each year. There is a continuing need to develop novel plant varieties that are less susceptible to damage or loss by such stresses. The present invention provides a system and method for generating plants having an increased resistance to plant stresses such as drought or adverse temperatures. Transgenic plants expressing a phosphoinositide-specific phospholipase C (PI-PLC) gene can show an unexpected and dramatic improvement in their capacity to tolerate a variety of stress conditions. Moreover, increased PI-PLC expression can further lead to marked increase in plant growth rates, maturation, and lipid content. The present invention encompasses transgenic plants having modifications with regard to PI-PLC pathways, such as altered PI-PLC levels, and plant products thereof.
Owner:NAT RES COUNCIL OF CANADA
Flavonoid 3',5'-hydroxylase gene functional marker for screening high-dihydroxyl catechin-content tea plant, as well as application and application method thereof
ActiveCN106755308AQuick filterAccelerate the pace of breedingMicrobiological testing/measurementDNA/RNA fragmentationHydroxylase genePlant variety
The invention belongs to the technical field of biology, particularly relates to a flavonoid 3',5'-hydroxylase gene functional marker for screening a high-dihydroxyl catechin-content tea plant, as well as application and an application method thereof, and discloses a functional marker for identifying the high-dihydroxyl catechin-content tea plant. The functional marker is applied to molecular marker-assisted selection, and can be used for rapidly screening a tea plant material with high dihydroxyl catechin content, thereby accelerating cultivation of a high-quality team plant variety. The functional marker has great theoretical significance and high economic value for molecular marker-assisted selection of a high-dihydroxyl catechin-content tea plant variety.
Owner:TEA RES INST CHINESE ACAD OF AGRI SCI
Method of producing a horticultural display
Owner:DUMMEN GRP BV
Grass and flower plant landscaping method adaptive to environmental changes
InactiveCN108432587AIncrease biodiversityEnhance and enrich biodiversityFlowers cultivationBulb cultivationLandscapingOrganism
The invention discloses a grass and flower plant landscaping method adaptive to environmental changes. The grass and flower plant landscaping method comprises the following steps: landscaping arrangement and designing: a step of determining of a landscaping range and a landscaping area; soil improvement; transplanting and planting; mixed planting through autumn sowing and spring sowing; and regular maintenance and resowing. According to the invention, a manner of combining transplanting with sowing is ingeniously adopted for establishment of landscaping plants, and plants with outdoor sowing difficulty and large sizes are transplanted, so the effects of highly-efficient establishment and effective control of the growing area of a plant variety with strong extension ability are achieved; amixed sowing manner is combined to construct a natural grass and flower landscape, so the landscaping performance is improved and enriched; growing grass and flower plants are more adaptable to a current environment; the survival ability of landscape plants is reinforced; the biological diversity of the landscaping area can be significantly improved; and abundant grass and flower plant varieties are utilized to attract more and various beneficial insects, so improvement of the health degree of an ecological system in the landscaping area is promoted, and a more beneficial human living environment is fedback and improved.
Owner:成都野趣生境园林景观设计有限公司 +1
Cutting cultivation method for acer palmatum
The invention discloses a cutting cultivation method for acer palmatum, which belongs to the field of horticultural production. The method comprises the following steps: inserting the cutting slips of the acer palmatum into a rooting agent cutting block which is dipped with a para-hydroxybenzoic acid containing 150 mg / l of IBA and having a mass ratio 0.001; and managing the cutting slips of the acer palmatum inserted into the rooting agent cutting block according to a full exposure cutting seedling cultivation mode, wherein calluses are formed after about 45 days; and the cutting slips of the acer palmatum inserted into the rooting agent cutting block can be transplanted after about 70 days. Tests show that the root-forming rate of the acer palmatum reaches 92 percent, the average formed root number of the cutting slips is 3.8 and an average root length is 3.5 cm. The cutting cultivation method for acer palmatum not only can quickly cultivate rare acer palmatum varieties in large scale under the condition of keeping the intrinsic excellent property of the original varieties, but also can provide a reference for plant varieties which are difficult to be cultivated by cutting, thereby having great popularization value.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
Plants stress tolerance correlation transcription factor GmWRKY54, encoding gene and application thereof
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Awning and rain-sheltering shed with climbing plants and building method thereof
The invention discloses an awning and rain-sheltering shed with climbing plants and a building method thereof. The shed comprises a support column and a shed top. The shed top comprises a support frame and a transparent water-proof plate which is paved on the support frame, and the outer surface of the transparent water-proof plate is provided with an attaching ring on which the climbing plants climb. The building method of the green shed of the invention comprises the following steps: 1) erecting the support column 1; 2) selecting the plant variety; 3) building the planting grooves 6; 4) filling the planting soil; 5) building the arched support frame; 6) paving the transparent water-proof plate; 7) arranging the attaching ring; 8) planting the plants. The natural shed of the invention not only shields sunlight and keeps the rain off, but also increases the city green coverage and beautifies the city environment. If the shed of the invention is erected on the rooftops of the high-rise buildings, the operation time of the air conditioner at the top layer is reduced, and the power supply is effectively saved. The invention is suitably popularized and applied to side pavements, open station platforms, overpasses and roofs on the rooftops of the buildings in cities.
Owner:王剑
A kind of grafting paste and its preparation method
InactiveCN102273380AImprove the survival rate of graftingPromote the survival rate of grafting and provide an effective guarantee for the survival rate of graftingBiocidePlant growth regulatorsFungicideMedicine
The invention discloses a grafting paste and a preparation method thereof, used for sealing a stock and a cion interface wound surface during plant variety improvement. The materials such as degradable paper and medicine pastes are adopted, wherein the medicine pastes are made of cotton and suck and moisten fungicide, humectant and growth regulator, and the grafting paste is in a strip shape. according to the invention, after a cion is inserted into a stock cut, a cotton paste made by drying after wetting mixed solution of antiseptic fungicide, the humectant and the growth regulator is pastedand applied to a wound, and then winding and splicing are carried out after pasting, thus labour and time are saved and the grafting paste is applicable to grafting of any method. After wrapping, microclimate humidity is subjected to self regulation in the interface, effective guarantee is provided for promoting grafting survival rate, the grafting paste is widely applied to agriculture technology and gardening and is applicable to different grafting methods of multiple plants, operation is simple and convenient, and labour efficiency can be obviously improved.
Owner:湖南吉兴农业科技开发有限公司
Plant breeding method
Methods for using genetic marker genotype (e.g., gene sequence diversity information) to improve the process of developing plant varieties (e.g., single cross hybrids) with improved phenotypic performance are provided. Methods for predicting the value of a phenotypic trait in a plant are provided. The methods use genotypic, phenotypic, and optionally family relationship information for a first plant population to identify an association between at least one genetic marker and the phenotypic trait, and then use the association to predict the value of the phenotypic trait in one or more members of a second, target population of known marker genotype. Methods for identifying new allelic variants affecting the trait are also provided. Plants selected, provided, or produced by any of the methods herein, transgenic plants created by any of the methods herein, and digital systems for performing the methods herein are also provided.
Owner:PIONEER HI BRED INT INC +1
Protein related to rice ear sprouting period and encoding genes and uses thereof
InactiveCN101353376AIncrease planting areaIncrease productionFungiBacteriaAgricultural scienceRibonucleotide synthesis
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
Plant stress tolerance related protein TaTPRPK1, encoding gene thereof, and application thereof
The invention discloses a plant stress tolerance related protein TaTPRPK1, an encoding gene thereof, and an application thereof. The protein provided by the invention is (a) or (b): (a) the protein is composed of an amino acid sequence represented by a sequence 1 in a sequence listing; and (b) the protein which is related to the plant stress tolerance, derived from the sequence 1, and obtained through substituting and / or deleting and / or adding to the amino acid sequence of the sequence 1 by one or some amino acid residues. The invention also discloses the encoding gene (TaTPRPK1) of the protein, and the application of the protein in breeding stress tolerance plants. The TaTPRPK1 can enhance the resistance of plants to NaCl, and the growth of roots. The TaTPRPK1 of the present invention lays the foundation for artificially controlling the expression of the anti-stress and stress resistance related gene, and plays an important role in breeding plant varieties with strengthened anti-stress and stress resistance.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Method for interplanting potato and corn in semihumid and semi-arid region
InactiveCN102461407AImprove compatibilityChanging the monoculture patternSeed and root treatmentHorticultureAridDisease
The invention relates to the technical field of agricultural planting, in particular to a method for interplaning potato and corn in a semihumid and semi-arid region. The invention comprises selection of planting mode, planting variety test and planting method and is characterized in that the planting mode that high pole crop corn and low pole crop potato are interplanted is selected, the planting variety test comprises that planting contrast test is carried out on potato Lk99, Longshu3 and Longshu6 and corn rich peasant 1, Shendan 16 and Yuyu 22, and the planting method is a high-yield variety planting method used for determining the planting contrast test. In the method provided by the invention, the potato Lk99 and the corn rich peasant 1 are preferably selected, firstly seeds of the two crops are subjected to pre-treatments such as disease and insect pest prevention and pregermination, then soil preparation, fertilization, interplanting and field management are carried out, and then harvesting is carried out. The invention has the beneficial effects that adverse factors of ecological environment to agricultural production are overcome, the traditional agricultural production mode is changed, specific yield is improved, and increase in production and synergism can be realized.
Owner:王宏凯
Soybean holy bean 9# NAC transcription factor gene GmST1 and application thereof
ActiveCN102660554AImprove salt/drought toleranceImproves salt/drought toleranceFermentationGenetic engineeringTransgeneGenetic engineering
The invention discloses a soybean holy bean 9# NAC transcription factor gene GmST1 and a plant expression vector of the gene GmST1 and further discloses application of the gene GmST1 in improving salt / drought resistance of arabidopsis thaliana and soybean plants. An experiment proves that compared with a non-transgenic plant, the salt / drought resistance of a transgenic plant is greatly improved. Therefore, the theory basis and the practice foundation are provided for improving the salt / drought resistance of plants through genetic engineering means. The soybean holy bean 9# NAC transcription factor gene GmST1 can be widely used for cultivating salt / drought resistant plant varieties.
Owner:SHANDONG UNIV
System, method and device for controlling illuminance and plant planting equipment
InactiveCN104798634AShorten the planting periodReduce manufacturing costClimate change adaptationGreenhouse cultivationIlluminanceEngineering
The invention relates to the technical field of household plant planting, and discloses a system, a method and a device for controlling illuminance and plant planting equipment, so as to shorten the plant planting cycle and reduce the production cost of plant planting. The system for controlling the illuminance of the plant planting equipment comprises a power supply part, an LED (light emitting diode) light source, an LED light source driving circuit, a plant variety inputting device, a timer and a control device, wherein the plant variety inputting device is used for inputting the information of the variety of the currently planted plant; the timer is used for recording the growth time of the variety of the currently planted plant; the control device is respectively connected with the LED light source driving circuit, the plant variety inputting device and the timer via signals, and is used for determining the growth time and illuminance curve corresponding to the variety of the currently planted plant according to the variety of the currently planted plant and the growth time and illuminance curve of the stored plant variety; a light emitting signal is outputted to the LED light source driving circuit according to the corresponding growth time and illuminance curve, so an LED light source can emit light with corresponding illuminance.
Owner:北京麻麻汇网络科技有限公司
Method for building and managing famous tea mechanical plucking tea garden
ActiveCN105684820AThe skeleton level is clearEvenly distributedCultivating equipmentsPlant cultivationPlant variety
The invention provides a method for building and managing a famous tea mechanical plucking tea garden, and belongs to the technical field of plant cultivation. The method is characterized by mainly comprising the steps of tea garden selecting, tea plant variety selecting, planting, tree crown cultivating and pruning, mechanical plucking period and remaining, maintaining of plucked tea tree crowns and the like, wherein frames of tea trees of the built tea garden are clear in layer, ventilative and transparent, growing branches are thick and uniform in distribution, the tree crowns are small in surface, stumps are uniform in thickness, and the density reaches about 1000 / m<2>; young sprouts of each tea tree grow on a regular and smooth tree crown surface, bud leaves are distributed uniformly, the density is proper and can reach about 2000 / m<2>, timely plucking is conducted, the raw material yield of famous tea can reach 70%-75%, and the plucking effect is good.
Owner:TEA RES INST CHINESE ACAD OF AGRI SCI
Double-layered growing method for lucern in mountainous areas of arid regions
InactiveCN102308720AIncrease success rateImprove effectivenessHorticultureAridOrthogonal test design
The invention relates to a double-layered growing method for lucern in mountainous areas of arid regions. The method is characterized in that: grandifoliate lucern is adopted as a main grown variety, an annual associated plant variety, such as rape, oat or highland barley, is chosen, and by means of a orthogonal test design and sowing order (position), growing spacing, growing depth, amount of applied fertilizer and other technical measures, and by utilizing the concealing and protectin effect generated by the double-layered growing method for the lucern in mountainous areas on lucern seedlings, the double-layered growing method can increase the current survival rate of the lucern seedlings. The double-layered growing method can promote the success of desert grassland improvement and artificial pasture construction under the environmental conditions of arid mountainous areas, becomes an optimal technology portfolio for growing lucern at medium and high altitudes in mountainous areas of arid regions, and greatly increases the survival rate of lucern in desert grassland restoration and artificial pasture construction and the yield of currently grown lucern in mountainous areas of arid regions at the altitude of 3000 to 1800 meters.
Owner:XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
Method of researching change of proteome of rice responding rice blast bacterial infection through iTRAQ technology
InactiveCN104820103AQuick and effective screeningStrong disease resistanceBiological testingDiseaseAgricultural science
The invention discloses a method of researching the change of a proteome of rice responding rice blast bacterial infection through an iTRAQ technology and belongs to the field of plant biotechnology. The method includes following steps: A. extracting a plant materials and leaf protein; B. performing further FASP enzymolysis, peptide fragment marking, SCX classification and LC-MS analysis to the protein sample obtained in the step A; C. performing data query and quantitative analysis to the result after the LC-MS analysis to obtain the change situation of the whole proteome during an interaction process between rice and rice blast bacteria. The method can quickly and effectively screening a candidate gene relative to disease resistance of rice, can be used for researching the candidate gene relative to the disease resistance of the rice well with the proteomics researching method, iTRAQ, which is high-throughput and accurate, can be widely used for researching and developing the characters of plant disease resistance and breeding new plant varieties being strong in disease resistance with combination of the transgenic technology.
Owner:SOUTH CHINA AGRI UNIV
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