347 results about "Plant variety" patented technology

The invention discloses a guidance planning method for ecological restoration of a side slope, and relates to the technical field of ecological restoration, and the main scheme is as follows: collecting multiple data of the side slope; calculating a slope stability index, and presetting a threshold value to judge whether the slope area is in a risk state; calculating a vegetation coverage area, and further calculating a plant suitability index; calculating a plant variety selection index, and further calculating a planting quantity; calculating the water and soil loss rate after restoration; calculating a plant adaptation comprehensive index; calculating an ecological restoration effect index and a vegetation density, and further obtaining a planting layout rationality index; calculating a soil stability index, further calculating a water and soil loss control index, and judging whether the soil loss control state is abnormal or not according to a preset threshold value; calculating a water and soil conservation effect index, further obtaining a vegetation growth comprehensive index, presetting a threshold value to judge the vegetation growth condition, and taking corresponding remediation measures; the method can effectively guarantee scientificity and effectiveness of ecological restoration of the side slope.

The invention discloses an application of a LOCOs12g07640 gene and an encoding protein thereof in improving drought stress resistance of plants. The drought resistance of rice germplasm resources of the inventor is investigated and researched, whole genome association analysis is combined with drought treatment expression level analysis results to screen out the gene shown in SEQ ID No.1, and the gene possibly has the capability of enhancing the drought stress tolerance of rice. By simulating drought stress conditions and observing phenotypic changes of overexpression and CRISPR knockout rice plants through direct seeding in dry land, the result shows that the drought stress tolerance of the overexpression LOCOs12g07640 gene plant in rice is enhanced, the rice plant with the gene knocked out and mutated is more sensitive to drought stress, and the drought stress resistance of rice is reduced. The rice stress resistance is improved and enhanced, the stress-resistant molecular breeding process is accelerated, the drought stress resistance of plants is improved or plant varieties with the drought stress resistance are cultivated, and the like.

The invention discloses an FtACA13 gene derived from tartary buckwheat as well as an expression vector and application of the FtACA13 gene. According to the invention, the FtACA13 gene is separated from tartary buckwheat; the polynucleotide sequence of the FACA13 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the FACA13 gene is as shown in SEQ ID NO.2. The FtACA13 gene is genetically transformed into buckwheat to obtain transgenic buckwheat hairy roots with the overexpressed FtACA13 gene, compared with hairy roots of a control group, the growth vigor and fresh weight of the fagopyrum tataricum hairy roots with the overexpressed FtACA13 gene under salt stress are increased, Na < + > accumulation in a plant body is remarkably low, it is proved that the FtACA13 gene can regulate and control the salt stress resistance of the plant, and the salt stress resistance of the fagopyrum tataricum hairy roots is improved. The invention has application prospects in the aspects of cultivating or breeding salt stress resistant plant varieties and the like.

The invention discloses a fusion protein and application thereof. Fusion expression is carried out on R ring binding domains from different species sources and a single gene base editor, and it is found that compared with a base editor before fusion, the base editing efficiency of the obtained fusion protein is improved, and a base editing window is widened. According to the method, the R-ring binding structure domain is fused with the single-gene base editor, so that the base editing efficiency can be improved, and an editing window can be adjusted, so that the method can be suitable for more base editing scenes. The method is beneficial to popularization and application of a single-gene editing technology, and has wide application prospects in prevention and / or treatment of diseases and construction of animal models or plant varieties.

The invention relates to the technical field of data analysis and support, and discloses a landscaping intelligent decision support system based on big data analysis. The system specifically comprises a greening data acquisition module used for acquiring growth state data and soil state data of greening plants in a transition area in a garden, and a morphological evolution degree evaluation module used for analyzing and evaluating morphological change conditions of the greening plants in the transition area in the garden, the resource balance degree evaluation module is used for analyzing and evaluating resource occupation conditions among green plants in a transition area in a garden; the decision support model construction module is used for constructing a decision support model; and the decision suggestion output module is used for converting decision support coefficients generated by the decision support model into maintenance suggestions of landscaping. The problem that in the prior art, when plant varieties need to be replaced in landscaping, the growth state and soil conditions of greening plants in a garden transition area cannot be comprehensively monitored in real time is solved.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention belongs to the technical field of ecological restoration, and particularly relates to an ecological environment data processing method and system for ecological restoration, and the method comprises the steps: a data collection module comprises a plant library, a mining area three-dimensional model and an environment monitoring platform; the data processing module is used for performing grid region division on the mining area three-dimensional model and processing data acquired by the environment monitoring platform; and the analysis matching module is used for screening and matching the optimal plant species for each grid unit. The method comprises the following steps: constructing a geographic model of a mining area, equally dividing the geographic model into a plurality of grid units, respectively acquiring environmental data for each grid unit, adaptively screening and matching plant varieties suitable for the grid units from a plant library according to different attributes such as soil, illumination, terrain and the like of the grid units; therefore, land resources are utilized to the maximum extent, plants are promoted to fully adapt to different environments of the mining area, the survival rate of vegetation is increased, and the ecological restoration and improvement effect of the mining area is achieved.

The invention discloses a mint McTTG2 gene as well as an expression protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the mint McTTG2 gene disclosed by the invention is shown as SEQ ID NO. 1, and the amino acid sequence of the expression protein of the mint McTTG2 gene is shown as SEQ ID NO. 2. The method comprises the following steps: constructing an expression vector of a mint McTTG2 gene, and transforming the expression vector into an arabidopsis thaliana mutant; and cultivating and screening to obtain a transgenic arabidopsis thaliana plant of which the procyanidine content is obviously increased. A transgenic experiment shows that after the McTTG2 gene is over-expressed in the mutant ttg2-7, the content of procyanidine is remarkably increased; compared with the mutant ttg2-7, the number of epidermal hairs on leaves of ttg2-7 / 35S:: McTTG2-1 and-2 strains is obviously increased, which is close to that of wild strains. Gene resources are provided for plant variety improvement, and cultivation of new mint varieties is facilitated.

The invention relates to application of a ZmNCED4 protein and a coding gene thereof in regulating and controlling low-temperature stress resistance of plants. The ZmNCED4 protein and the coding gene thereof have obvious influence on low-temperature stress resistance of plants (especially corn), and the low-temperature stress resistance of the corn can be improved by inhibiting expression of the coding gene of the ZmNCED4 protein in the corn. The application provides a good germplasm resource for cultivating new varieties of low-temperature-resistant plants, and lays a theoretical foundation for researching a mechanism of responding to stress signals and a molecular mechanism of tolerating unfavorable environments of the plants.

The invention relates to the technical field of gene breeding, in particular to an application of FveWRKY50 protein and a coding gene thereof in regulation and control of plant anthracnose resistance, the FveWRKY50 gene is knocked out from strawberries and overexpressed, it is verified that the strawberry FveWRKY50 gene can negatively regulate and control the anthracnose resistance, and by reducing the expression quantity of the FveWRKY50 protein in plants, the anthracnose resistance of the plants can be negatively regulated and controlled. The anthracnose resistance of plants can be effectively improved. The verification of the anthracnose resistance function of the FveWRKY50 gene provides a new gene target and resource for cultivating anthracnose resistance improving plant varieties, and lays a certain theoretical foundation for researching the anthracnose resistance responding mechanism and the adverse environment resisting molecular mechanism of plants.

The invention relates to the technical field of plant genetic engineering, in particular to application of ZmAAAP59 protein and a coding gene thereof in regulation and control of high temperature stress tolerance of corn, the ZmAAAP59 gene is knocked out in corn, it is determined that a mutant of the ZmAAAP59 gene has an obvious high temperature resistance phenotype, it is verified that the corn ZmAAAP59 gene can negatively regulate and control plant heat resistance, and by reducing the expression quantity of the ZmAAAP59 gene in the plant, the corn ZmAAAP59 gene can negatively regulate and control plant heat resistance. The heat resistance of plants can be effectively improved. The discovery of the heat-resistant function of the ZmAAAP59 gene provides a new gene target and resource for cultivating heat-resistant plant varieties, has important significance on research on a heat-resistant molecular mechanism of corn, and lays a certain theoretical foundation for research on a high-temperature stress response mechanism and a molecular mechanism for resisting adverse environments of plants.

The invention discloses a polypeptide and application thereof in regulation and control of plant development, and relates to the technical field of biology. The invention specifically discloses any polypeptide from A1) to A3) as shown in the following: A1) a polypeptide with an amino acid sequence as shown in SEQ ID NO: 1; a2) a polypeptide which is obtained by substitution and / or deletion and / or addition of more than one amino acid residue on the amino acid sequence as shown in SEQ ID NO: 1 and has the function of regulating plant growth and development; and A3) a fused polypeptide obtained by connecting a tag to the N end and / or C end of A1) or A2). The polypeptide can effectively regulate and control root system growth and root regeneration of plants, can be well applied to improvement of plant varieties, and has important practical value for plant breeding work.

The invention discloses application of a CSA1 gene in regulating and controlling branching angles and petiole angles of leguminous plants and creating close-planting-resistant high-yield plant type leguminous plants. According to the invention, a CSA1 gene is cloned from soybean, and a CRISPR-Cas9 system is further utilized to edit the CSA1 gene, so that two different mutants are obtained. A result shows that the branching angle and the petiole angle of a mutant plant are remarkably reduced, and the yield of the mutant is remarkably higher than that of a wild type under a high-density planting condition, so that the CSA1 gene can regulate and control the branching angle and the petiole angle of the leguminous plant and create a close-planting-resistant leguminous plant, an important gene resource is provided for molecular breeding of the leguminous plant, and the application prospect is wide. And an effective means is provided for creating close-planting-resistant high-yield leguminous plants.

The invention discloses an application of alfalfa NACsa3 protein in cultivation of salt-tolerant plants. The protein provided by the invention is named as MtNACsa3 protein, is derived from medicago truncatula, and is the protein as shown in SEQ ID NO: 1. The invention also provides a biological material related to the MtNACsa3 protein. The invention also protects a method for cultivating the plant with improved salt tolerance, and the method comprises the following step: introducing the MtNACsa3 protein related biological material into a receptor plant to obtain the transgenic plant with improved salt tolerance. The invention also protects a method for cultivating the plant with reduced salt tolerance, and the method comprises the following steps: inhibiting the expression of the nucleic acid molecule for coding the MtNACsa3 protein in the target plant to obtain the transgenic plant with reduced salt tolerance. The invention provides important normal forms and gene resources for cultivating salt-tolerant plant varieties.

The invention discloses a low-cost flower sea plant screening method and a landscape scheme generation method. The low-cost flower sea plant screening method comprises the following steps: acquiring indexes for evaluating low-cost flower sea plants; determining the weight of each index; training to obtain a low-cost flower sea plant screening model; and forming a variety library of the flower sea plants in the region according to the variety information of the flower sea plants in the to-be-screened region. Constructing a flower sea plant landscape effect evaluation system, collecting a flower sea plant landscape plan, scoring the plan effect according to the evaluation system, and constructing a flower sea landscape configuration training set; training the model to obtain a landscape scheme generation model; outputting coordinates by adopting a particle swarm algorithm, and generating a planar graph through plant picture information. According to the method, an evaluation system is established according to a low-cost flower sea plant screening target, a low-cost flower sea plant screening model is constructed through a neural network, a low-cost flower sea plant recommendation list is obtained, a low-cost flower sea landscape configuration scheme is further generated according to site basic conditions and environmental conditions, and a decision basis is provided for flower sea landscape construction.

The invention discloses an ecological monitoring system based on oblique photography and ortho-images, and relates to the technical field of ecological monitoring, and the system comprises the steps: extracting multi-angle shot images of all plants in a region of interest where ecological monitoring is located based on ortho-image resources and oblique photography materials of the region of interest where ecological monitoring is located; generating variety information, growth information, water resource information and soil information of each plant, and further identifying the growth change trend of each plant variety based on the variety information and historical growth information of each plant identified by historical multi-angle shot images of each plant; and further identifying the difference condition of the growth change trend in each plant variety, analyzing and generating a water resource dependency relationship and a soil dependency relationship of each plant variety based on the water resource information and the soil information, and finally outputting an ecological monitoring report. According to the invention, the growth state and the ecological environment of the plant are accurately monitored by integrating image data from multiple angles, and the optimal distribution of resources is ensured.

The invention discloses a plant variety zoning and yield prediction method, system and device, a medium and a product, and relates to the field of plant variety distribution analysis, and the method comprises the steps: obtaining ecological factor data of a research region and point location data of existing distribution points of a target plant variety based on literature mining; the ecological factor data comprises a contemporary climate variable, a future climate variable, a soil variable and a terrain factor variable; the point location data comprises distribution point data and yield data; according to the ecological factor data of the research area and the point location data of the existing distribution points of the target plant variety, determining suitable growth area distribution of the target plant variety in the research area by adopting a maximum entropy model; and predicting the yield of the target plant variety in the research area by adopting a machine learning model according to the future climate variable and the soil variable of the research area. The method for predicting the suitable growth area and the yield of the target plant variety is established, and the precision is improved.

The invention discloses a KCR1 mutant suitable for plant potassium ion steady state regulation and application thereof, and relates to the technical field of bioengineering, and the KCR1 mutant at least has T136A and G140A mutations on the basis of light-operated potassium ion channel protein KCR1. The invention also discloses a gene for coding the KCR1 mutant. The invention also discloses application of the KCR1 mutant or gene in plant culture, cultivation of new plant varieties or preparation of plant culture products. The KCR1 mutant disclosed by the invention has a blue-shift excitation spectrum compared with a wild type, can realize accurate regulation and control of plant potassium ion flow through illumination regulation and control, and has a relatively great application value in the aspects of light-controlled plant cultivation, plant potassium ion channel research and the like.

The invention relates to the technical field of plant variety cultivation, in particular to new application of an RcRBL1 gene and a method for preventing or treating black spot and gray mold of Chinese roses. According to the application of the RcRBL1 gene in preventing or treating the black spot and the gray mold of the Chinese rose, the capability of resisting the black spot and the gray mold of the Chinese rose plant can be remarkably improved by reducing the expression of the RcRBL1 gene in the Chinese rose plant, an effective method is provided for preventing and treating the black spot and the gray mold of the Chinese rose, and the cultivation cost of the Chinese rose is reduced; and the market economic benefits are improved.

The invention discloses a cotton GhLAC14-3 gene and application of the cotton GhLAC14-3 gene in regulation and control of verticillium wilt resistance of plants. According to the invention, the GhLAC14-3 gene is separated from cotton, the nucleotide sequence of the GhLAC14-3 gene is shown as SEQ ID No.1, and the amino acid sequence of the encoded protein of the GhLAC14-3 gene is shown as SEQ ID No.2. The invention further performs preliminary research on the function of the cotton GhLAC14-3 gene in cotton verticillium wilt resistance, and the result shows that after the GhLAC14-3 gene in cotton is silenced, the verticillium wilt resistance of a cotton seedling plant is obviously reduced; after the GhLAC14-3 gene is over-expressed in arabidopsis thaliana, the verticillium wilt resistance of the arabidopsis thaliana is remarkably improved, so that the resistance of a GhLAC14-3 gene positive regulation plant to verticillium dahliae is determined. The invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention relates to the field of urban landscaping management, and discloses an urban landscaping monitoring and management system based on the Internet of Things, the monitoring and management system comprises a division module, a plurality of monitoring modules and an analysis module, and a landscaping area is divided into a plurality of target areas according to plant varieties through the division module; monitoring the corresponding target area through a monitoring module, and obtaining plant monitoring data and environment data of plants in the corresponding target area; analyzing the plant monitoring data through an analysis module to obtain the growth stage of the plant, performing analysis according to the growth stage of the plant, the plant monitoring data and the environment data, and judging whether the plant in the corresponding target area is abnormal or not according to analysis; the accuracy of plant health state evaluation is improved, so that supervisors can know the health condition of plants in each target area in the greening area more intuitively, and plant abnormity can be found in time for corresponding processing.

The invention discloses a transcription factor ZmbHLH180 gene as well as an encoded protein and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the corn ZmbHLH180 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2. The invention provides a transcription factor ZmbHLH180 gene, the CDS full length of the gene is 864bp, 287 amino acids are encoded, the gene is mainly positioned in a cell nucleus, and the expression of a chlorophyll degradation gene NYC1 and the senescence of leaves can be obviously promoted by over-expressing the ZmbHLH180 gene in arabidopsis thaliana; the trans-ZmbHLH180 gene overexpressed corn can remarkably promote leaf greening under drought stress, a certain technical foundation is laid for regulation and control of the plant growth cycle, a choice is provided for genetic improvement of new plant varieties, and the application value is important.

The invention relates to application of a ZmbZIP68 protein and a coding gene thereof in regulation and control of heat resistance of corn. It is found that the corn ZmbZIP68 gene can positively regulate the heat resistance of plants, and the heat resistance of the plants can be effectively improved by overexpressing the expression quantity of the ZmbZIP68 gene. The discovery of the heat-resistant function of the ZmbZIP68 gene provides a new gene target and resource for cultivating heat-resistant plant varieties, has important significance on research on a heat-resistant molecular mechanism of corn, and lays a certain theoretical foundation for research on a high-temperature stress response mechanism and a molecular mechanism for resisting adverse environments of plants.

The disclosure refers to an observation trail method for determining a resistance of a plant variety to one or more pest species which infests a field plant of the plant variety, the method comprising Providing experimental plants of a plant variety (100); Arranging the experimental plants next to pest-infested field plants at a first point time (150), wherein the pest-infested field plants are of the same plant variety or another plant variety as the experimental plants and wherein the pest-infested field plants are in a field where field plants of the same or another plant variety is growing, wherein the pest-infested field plants are infested with a pest species to which resistance of the experimental plants is to be determined; Classifying a physical state of the experimental plants at a second point of time (400); and Classifying the physical state of the experimental plants at at least a further point of time being after the second point of time (500).

The invention belongs to the technical field of degenerated sand land treatment, and particularly relates to a degenerated sand land vegetation restoration method based on a donation soil seed bank. Comprising the steps of donation soil seed bank acquisition, donation soil seed bank soil matrix improvement and donation soil seed bank transplanting and laying. The method comprises the following steps: firstly, selecting a top-level or near-top-level natural community of a habitat vegetation succession sequence as a donation source area of a donation soil seed bank, obtaining surface soil of a habitat earth surface containing the seed bank, wherein transplanted soil contains a soil seed bank, nutrient propagules such as root systems and subterranean stems of plants, soil organisms and the like; and then transplanting to the surface of the degraded sand land, realizing vegetation recovery of the degraded sand land by utilizing the transplanted soil seed bank and soil through a natural germination and growth process, and recovering the vegetation coverage to the community level of the donation source area in the second year and the third year after implementation, and enabling the plant variety number to be close to the species number of the donation source area.

The invention discloses an application of a millet SiPYL3 gene and an encoding protein thereof in regulation and control of plant stress resistance and / or yield. The invention relates to the field of plant genetic engineering, and provides application of SiPYL3 protein or a coding gene thereof in regulation and control of plant salt resistance and / or drought resistance and / or yield. The amino acid sequence of the SiPYL3 protein is as shown in SEQ ID No. 1. According to the invention, the coding gene of the SiPYL3 protein from millet is introduced into rice, so that the salt tolerance of the obtained transgenic line is improved, the drought resistance is reduced, and the yield is increased. Therefore, the SiPYL3 gene can positively regulate the salt tolerance and yield traits of the plant and negatively regulate the drought resistance of the plant. The invention has important significance for cultivating salt-resistant and high-yield plant varieties.

A stevia cultivar with super high Rebaudioside A content, designated ‘320032’, is disclosed. Another embodiment relates to the plant parts of stevia cultivar ‘320032’, to the plants of stevia ‘320032’ and to methods for producing a stevia plant produced by crossing the cultivar ‘320032’ with itself or another stevia variety, including methods using marker assisted breeding. Another embodiment further relates to hybrid stevia seeds and plants produced by crossing the cultivar ‘320032’ with another stevia cultivar. Twelve highly polymorphic SNPs loci and the corresponding genomic sequences used to identify plant variety ‘320032’-derived plant materials are also disclosed.

The invention provides a construction method of an ecological buffer zone of a reservoir hydro-fluctuation belt in a southwest karst region. The construction method comprises the following steps: S1, dividing a suitable recovery area of the hydro-fluctuation belt; s2, slope reconstruction and substrate improvement; s3, plant variety selection and configuration; s4, planting and maintaining the plants. The original soil condition of the hydro-fluctuation belt is improved through substrate improvement, the tolerance of plants to water level fluctuation is ensured through differentiated seed selection of the regions I-IV, the survival rate of vegetation in karst barren soil is increased, and the restoring capacity of an ecological system of the hydro-fluctuation belt is enhanced. According to the method, the storage submerged plant buffer pond is transformed on the lower portion of the hydro-fluctuation belt area or the artificial aquatic plants are arranged, the ecological buffer space of the hydro-fluctuation belt is prolonged, the storage pollution purification and retaining capacity is improved, and good application prospects are achieved.

The invention discloses an application of a rice OsWRKY15 gene and an encoding protein thereof in regulating and controlling drought stress resistance of plants. A knockout plant and an overexpression plant of the OsWRKY15 gene are respectively constructed, phenotypes of wild rice, the knockout plant of the OsWRKY15 gene and the overexpression plant of the OsWRKY15 gene are observed under drought treatment, and results show that compared with the wild rice, the wilting and withering degree of the knockout plant of the OsWRKY15 gene is larger than that of the wild rice, and the survival rate of the knockout plant of the OsWRKY15 gene is smaller than that of the wild rice; the wilting and withering degree of an overexpressed plant of the OsWRKY15 gene is smaller than that of wild type rice, and the survival rate of the overexpressed plant is larger than that of the wild type rice. The invention proves that the OsWRKY15 gene has the capability of positively regulating the drought stress resistance of rice, and has application prospects in the aspects of improving the drought stress resistance of plants, cultivating drought stress resistant plant varieties and the like.

The invention relates to the technical field of plant breeding, in particular to a primer combination for detecting a regulatory element and an exogenous gene of a transgenic plant and application of the primer combination. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. 22-63. The primer combination comprises a nucleotide sequence as shown in SEQ ID NO. The application comprises: (1) transgenic plant variety germplasm supervision or transgenic plant component screening in non-transgenic varieties; (2) screening transgenic ingredients of the plant product; (3) auxiliary screening in the plant breeding process; and (4) tracing plant varieties. The invention provides a primer combination which can be applied to detection of regulatory elements and exogenous genes of transgenic plants, can realize detection of a plurality of regulatory elements and exogenous genes in a reaction system, and can meet high-throughput screening of components of the transgenic plants. The method is used for monitoring illegal planting of transgenic plants and detecting transgenic components of related plant products.