10 results about "Leaf base" patented technology

The application discloses a method for dynamically inverting a net photosynthetic rate of a plant leaf based on a transmission spectrum, and relates to the technical field of testing and analysis. Different from the reflection spectrum, the transmission spectrum directly penetrates the leaf tissue, contains absorption and scattering information of the light by the internal substances (chlorophyll, water and structure) of the leaf, the distance between the spectrum detector and the leaf is close to 0, is not affected by environmental conditions, and can more directly reflect the optical characteristics related to the core process of photosynthesis. The method aims at overcoming the shortcomings of the prior art, providing a fast, non-destructive, efficient and accurate Pn dynamic monitoring technical means for crops of different photosynthetic types, and serving the needs of precision agricultural management.

The invention discloses an application of a GmCDC48c gene and an encoded protein thereof in regulation and control of soybean Cd resistance and accumulation. The soybean GmCDC48c gene is knocked out through gene editing to obtain a knockout mutant, under the stress of Cd, compared with WT, a mutant plant is short and small, the leaf base is red, the whole leaf curls, but the Cd concentration of grains is reduced, and Cd accumulation of soybeans is reduced. Therefore, the GmCDC48c gene is a regulatory factor for coding Cd resistance and accumulation, and can provide a new gene target for soybean genetic breeding.

Disclosed herein is a system and methods for leaf detection and extraction. The extracted leaf may be used for leaf water potential analysis. In some embodiments, the method comprises identifying the leaf from a point cloud based on an image, determining a pose of the leaf based on the point cloud, and cutting and storing the leaf based on the pose of the leaf.

The application discloses a SPAD value prediction method and system based on fresh leaf color recognition, and the method comprises the following steps: controlling a shooting device to collect fresh leaf real-time samples and uploading the samples; performing sample pretreatment to obtain fresh leaf sample characteristic values; and predicting the SPAD value of the fresh leaf based on a pre-deployed fresh leaf SPAD value prediction model and the sample characteristic values, to obtain the SPAD value prediction of the fresh leaf real-time sample. The application controls the real-time collection and uploading of fresh leaf images on site, and completes sample pretreatment by using image processing technology, and then combines the pre-deployed fresh leaf SPAD value prediction model, so that the low-cost real-time prediction of the SPAD value of the whole fresh leaf in a complex field environment is realized.

The invention relates to the technical field of plant tissue culture, in particular to an onion tissue culture seedling subculture method which comprises the following steps: S1, disinfecting and aseptically germinating onion seeds, and culturing until the leaf bases of seedlings change color and expand; s2, cutting off leaves and root systems of the seedlings obtained in the step S1 under a sterile condition, and retaining expanded parts of leaf bases to obtain tissue blocks containing growing points; s3, cutting the tissue block into small blocks; and S4, inoculating the cut tissue blocks into an induction culture medium, and culturing and inducing the tissue blocks to regenerate and form complete tissue culture seedlings. According to the onion tissue culture seedling subculture method, seeds with disinfected surfaces are used as starting materials, pollution is controlled from the source, and the initial pollution rate is reduced; by selecting a seedling growing point at a leaf base discoloring and expanding stage as an explant, the cell meristematic ability is strong, and the inductivity is high; material taking treatment is optimized according to the morphological characteristics of onions, the problem of lateral branch subculture is solved, the process standardization degree is high, and the propagation coefficient is high.

This invention provides a processing method to enhance the usability of medium-grade tobacco leaves, comprising the following steps: (1) vacuum rehydrating the medium-grade tobacco leaves and then cutting the leaf base to obtain leaf body and leaf base; (2) performing a first and second moistening treatment on the leaf body to obtain processed material; (3) performing a first and second moistening treatment on the leaf base, and merging the obtained material with the second leaf body processed material to obtain a combined material; (4) performing vacuum rehydration, moistening, bio-enzyme application, and alcohol treatment on the combined material to obtain alcohol-treated material; (5) mixing the alcohol-treated material with the first leaf body processed material, and performing a first moistening, a second moistening, and inactivation. The processing method provided by this invention is based on the leaf threshing and re-drying process, which can improve the sensory quality defects of medium-grade tobacco leaves, enhance aroma quality, significantly improve its formulation applicability and comprehensive utilization efficiency, revitalize existing low-quality tobacco leaf resources, and provide core technical support for the efficient utilization of raw materials in the tobacco industry.

The application discloses a kind of transgenic rootlet induction method of Drynaria fortunei and application thereof, the induction method includes the following steps: S1: picking the third to fourth piece of leaf under Drynaria fortunei terminal bud as explant;S2: preparation of Agrobacterium rhizogenes infection solution and Agrobacterium rhizogenes bacteria;S3: the leaf blade collected in step S1 is placed in the Agrobacterium rhizogenes infection solution obtained in step S2, cut off petiole, expose leaf base, the Agrobacterium rhizogenes bacteria obtained in step S2 is applied in leaf base, to obtain the explant leaf base after inoculation;S4: the explant leaf base after inoculation in step S3 is inserted into sterilized culture medium and is cultured, i.e. hairy root can be induced;The culture medium includes vermiculite and soil.The induction method culture condition is simple, easy to operate, and the transgenic rootlet induction rate is greater than 80% by PCR detection, the induction method effectively breaks through the technical barrier of Drynaria fortunei transgenic system establishment, and the cost is low using pure vermiculite culture medium.

The invention belongs to the technical field of plant measuring tools, and discloses a plant leaf measuring scale, which comprises a main board, a moving assembly, a placing assembly and a rubber belt, the moving assembly comprises a limiting rod fixedly arranged on the surface of the main plate and a plurality of sliding blocks arranged on the limiting rod in a sliding mode, fixing rods are arranged on the two sets of sliding blocks close to the two ends of the limiting rod, the two ends of the rubber belt are fixed to the two sets of fixing rods respectively, scale marks are arranged on the rubber belt at equal intervals, and the whole rubber belt is evenly divided into a plurality of sections. And corresponding proportion values are correspondingly arranged below the scale marks. A leaf to be measured can be fixed in the main plate through the placing assembly, the fixing rod is aligned with the tail end of a leaf stalk and the tip end of the leaf tip, and the stalk-leaf ratio can be obtained by observing the ratio value of the leaf base to the rubber band. The fixing rod at the tail end of the blade handle is moved to the blade base, the base tip ratio can be obtained by observing the proportion value corresponding to the widest position of the blade, important index data can be rapidly and visually measured, and the burden of workers is relieved.

The invention discloses an induction method and application of a cinnamomum camphora transgenic hairy root. The induction method comprises the following steps: S1, picking third to fourth leaves under a terminal bud of cinnamomum camphora as explants; s2, preparing an agrobacterium rhizogenes infection solution and agrobacterium rhizogenes thalli; s3, the leaves collected in the step S1 are placed in the agrobacterium rhizogenes infection liquid obtained in the step S2, petioles are cut off, leaf bases are exposed, agrobacterium rhizogenes obtained in the step S2 are smeared on the leaf bases, and inoculated explant leaf bases are obtained; s4, inserting the leaf base of the explant inoculated in the step S3 into a sterilized culture medium for culture, so as to induce hairy roots; the culture medium comprises vermiculite and soil. The induction method is simple in culture condition and easy and convenient to operate, and the transgenic hair root inductivity gt is detected through PCR; the induction method effectively breaks through the technical barrier of establishment of a cinnamomum camphora transgenic system, adopts a pure vermiculite culture medium, and is low in cost.

The invention discloses a tissue culture method of polygonatum odoratum, and relates to the technical field of plant tissue culture. The tissue culture method of the polygonatum odoratum comprises the following steps: performing dedifferentiation culture on a new root explant of the polygonatum odoratum or a leaf base explant of a tissue culture seedling leaf in a dedifferentiation culture medium to obtain a callus; carrying out multiplication culture on the callus in a multiplication culture medium; performing bud differentiation culture on the proliferated calluses in a bud differentiation culture medium, and inducing and differentiating adventitious buds; the bud differentiation culture medium takes an MS culture medium as a basic culture medium and comprises 3.0-4.0 mg / L of 6-BA, 0.1-0.5 mg / L of NAA, 20-30 g / L of a third carbon source and 5-7 g / L of agar; performing root differentiation culture on the adventitious buds in a root differentiation culture medium to obtain tissue culture seedlings. The tissue culture method is high in induction rate, short in period, high in operability and easy to popularize and repeat.