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64results about How to "Increase planting costs" patented technology

Agrobacterium tumefaciens-mediated barley stem apex transformation method

The invention discloses an agrobacterium tumefaciens-mediated barley stem apex transformation method. The transformation method comprises the following steps: A) culturing barley stem apex and constructing a carrier, wherein the specific steps are as follows: 1) selecting barley caryopses, removing hulls, stripping mature embryos and downwards placing scutella on a germination culture medium for germination; and 2) obtaining a maize ubiquitin promoter and the related sequence from an intermediate carrier pMBL-3 and a green fluorescent protein gene (GFP) through enzyme digestion and a PCR (polymerase chain reaction) method; and B) mediating the genetic transformation of the barley stem apex by utilizing agrobacterium tumefaciens, wherein the specific steps are as follows: 1) obtaining the barley stem apex; 2) dip-dyeing the stem apex; 3) using the stem apex and the agrobacterium tumefaciens GV3101 to co-culture the culture medium: taking out the stem apex which is dip-dyed with the agrobacterium tumefaciens and sucking up surface bacterial liquid with sterile filter paper; 4) performing restoring culture on the stem apex by using a restoring culture medium; 5) screening the stem apex on a screening the culture medium; and 6) vernalizing and cultivating a regenerated plant so as to obtain a candidate transformed plant. The method is easy to operate and simple to operate and has the advantages of short cycle, high efficiency and simple procedure, and the result is reliable.
Owner:HUAZHONG AGRI UNIV

Agrobacterium tumefaciens-mediated barley leaf base transformation method

The invention belongs to the technical field of plant transgenosis, and particularly relates to an agrobacterium tumefaciens-mediated method for obtaining a transgenic plant by adopting a barley seedling leaf base to induct a callus. The agrobacterium tumefaciens-mediated method has the key points as follow: a barley leaf base serves as an explant to induct the callus; an agrobacterium tumefaciens-mediated genetic transformation method is adopted, a Bar gene is used as a screening gene, and a herbicide of glufosinate ammonium is used as a selective agent; the transformed callus and differentiated plants are screened; a foreign gene is confirmed to be integrated into a barley genome through PCR and Southern hybridization analysis; a transgenic barley plant is obtained. Compared with the prior art, the invention has the advantage that the explant (callus) of the transgenic plant can be supplied all the year round; compared with a classic transformation method in which a young embryo is taken as an acceptor, the agrobacterium tumefaciens-mediated method has the advantage that the acceptor obtaining time is shortened to be 3 days form 150 days, and the time limit of young embryo sampling is eliminated; compared with a stem tip transformation method, the agrobacterium tumefaciens-mediated method is lower in technical requirement and simpler to operate.
Owner:HUAZHONG AGRI UNIV

Maifanite-containing double-effect multi-component organic/inorganic compound mixed fertilizer and manufacturing method thereof

The invention discloses a maifanite-containing double-effect multi-component organic / inorganic compound mixed fertilizer and a manufacturing method thereof. The maifanite-containing double-effect multi-component organic / inorganic compound mixed fertilizer is low-carbon and environment-friendly and produced by organic combination of natural components including humic acid, maifanite, bentonite, potassium oxide, ammonium phosphate, ammonium chloride, borax, zinc sulfate, aluminum potassium sulfate, and traditional Chinese medicines for agricultural use (including aloe, Tripterygium wilfordii, onion juice, tobacco stem, Radix Glycyrrhizae, Aconitum kusnezoffii and Pyreyhrum), biological enzyme activators and a PASP plant growth promoter at normal temperature and pressure. The manufacturing method comprises the following steps: grinding multiple raw materials in the formula to powder of about 120 to 200 meshes, thoroughly mixing according to the given ratio in a stirring machine, conveying to a granulating machine through a conveyor, chelating by spraying a PASP aqueous solution and curing and drying at a high temperature by use of a drying machine, sieving to obtain the fertilizer product which has a diameter of 2.5 to 4.5 mm, a strength of 5 to 6 n and a free moisture of less than 10%, and quantitatively bagging after the product passes the quality test. The fertilizer product has complete nutrients and balanced nutrition, is rich in organic matters and amino acids, can activate and utilize residual fertilizers in the soil, improves yield and quality of crops, produces green and organic agricultural products, helps increase farmer's incomes, well solves the problems and difficulties caused by long-term use of chemical fertilizers in agricultural production and farming, overcomes the defects of chemical fertilizers, and truly meets the national industrial policy requirements for economic and social benefits of green, organic and environment-friendly sustained- / controlled-release fertilizers.
Owner:曹聿哲

Method for conducting crop rotation of asparagus lettuce, waxy corn, cowpea and winter cabbage in annual-four-crop mode in Chongqing

A method for conducting crop rotation of asparagus lettuce, waxy corn, cowpea and winter cabbage in annual-four-crop mode in Chongqing. The method includes reasonably planting the first-crop asparagus lettuce in the crop rotation period, wherein sowing and seedling raising are conducted in the period from November 20 to the end of November of the previous year, the transplanting time ranges from January 1 to January 20 of the ensuing year, and the marketing time ranges from March 15 to the beginning of April; planting the second-crop waxy corn, wherein the sowing and seedling raising time ranges from January 20 to the end of January, the transplanting time ranges from February 15 to the end of February, and the marking time ranges from the end of May to the mid of June; planting the third-crop autumn cowpea, wherein the direct seeding time ranges from June 20 to the end of June, and the marketing time ranges from August 1 to the end of September; planting the fourth-crop winter cabbage, wherein the sowing and seedling raising is conducted at the end of June, the transplanting time ranges from August 20 to the end of August, and the marketing time ranges from the end of November to the beginning of December. Due to the fact that scientific interplanting method is adopted to select excellent species, the growing mode is improved, materials are taken locally, waste is reused, the sowing and the planting are conducted in advance, marketing is conducted in advance, the production value is greatly increased, and the method meets the market demand and has obvious economical benefit.
Owner:郑国福

Fructus trichosanthis yield increase and fertilizer application method

The invention relates to the technical field of agricultural planting, in particular to a fructus trichosanthis yield increase and fertilizer application method. Specifically, for annual fructus trichosanthis, 500-600 kg of decomposed pig manure, 60 kg of nitrogen fertilizer, 20 kg of phosphate fertilizer and 50 kg of potash fertilizer are applied for a year round; for two-year-old fructus trichosanthis, 500-550 kg of decomposed pig manure, 55 kg of nitrogen fertilizer, 20 kg of phosphate fertilizer and 30 kg of potash fertilizer are applied for a year round; for three-year-old fructus trichosanthis, 500-520 kg of decomposed pig manure, 52 kg of nitrogen fertilizer, 22 kg of phosphate fertilizer and 35 kg of potash fertilizer are applied for a year round; for fructus trichosanthis which is over three years old, 300-350 kg of decomposed pig manure, 30 kg of nitrogen fertilizer, 15 kg of phosphate fertilizer and 15 kg of potash fertilizer are applied for a year round. According to the method, by reasonably controlling the varieties of fertilizers, the fertilizer proportions and fertilizer application methods in the fructus trichosanthis planting period, chemical fertilizer application can be reduced, the planting cost is not increased, the yield of fructus trichosanthis can be increased, and the soil ecological environment is improved.
Owner:蚌埠清菲农业科技有限公司

Medical stone dual-effect multi-element organic fertilizer and preparation method thereof

The invention discloses a medical stone dual-effect multi-element organic fertilizer and a preparation method thereof. The medical stone dual-effect multi-element organic fertilizer which is low in carbon and environment friendly is produced by organically blending natural humic acid, medical stone, calcium stone, bentonite, potassium oxide, ammonium phosphate, magnesium sulfate, borax, zinc sulfate, ferrous sulfate, aluminum potassium sulfate, agricultural traditional Chinese medicines (veratrum nigrum, celastrus angulatus, garlic juice, tobacco stalk, liquorice, Chinese stellera root and cyrtomium rhizome), a polyaspartic acid (PASP) plant nutrient accelerator and the like under the condition of normal temperature and normal pressure. The preparation method comprises the following steps of: crushing and milling the raw materials in the formula into powder of which the grain size is about 120 to 200 meshes; uniformly mixing the powder in a stirrer according to proportion; conveying the mixture to a granulator through a conveyor; after spraying and chelating the mixture through PASP and water mixed solution, granulating the mixture; after curing and drying the mixture with a dryer at high temperature, screening the grains, wherein the fertilizer product is 2.5 to 4.5mm in grain size, 5 to 6 Newtons in strength and less than 10 percent in free moisture; and after the product is checked to be qualified, metering and filling the product in bags. Fertilizer and medicine are integrated in the product; the product is comprehensive in nutrient elements, uniformly supplies fertilizer and is rich in organic substances and amino acids; residual fertilizer in the soil can be activated and used; the yield and the quality of crops are improved; green and organic agricultural products are produced; income of farmers is increased; the defect and difficulty brought to agricultural production and plantation by applying chemical fertilizers for a long time can be well overcome; the defects and the disadvantages of the chemical fertilizers are overcome; and the industry policy requirement of China on the economic benefit and social benefit of a green, organic and environment-friendly sustained or controlled release fertilizer is really met.
Owner:QIQIHAER JINDINGFENG MEDICAL STONE FERTILIZER IND CO LTD

Storage method of broad beans

The invention mainly relates to the field of storage of agriculture products, and discloses a storage method of broad beans. The storage method comprises the following steps of performing picking, performing drying, and performing storage. The method is simple, batched treatment and storage can be performed, and after the broad beans are stored for one year, the color change rate of the broad beans is reduced to 1% or below, and the color and the nutrients of the broad beans can be maintained. The sprouting rate is 97.3%, the planting cost is saved, and the economic returns are increased by 11.6%. After the broad beans are picked, the broad beans and broad bean pods are picked together, so that the protection effect of the broad bean pods is sufficiently achieved. After the broad beans are picked, the broad bean pods are continuously and repeatedly sprayed with a vitamin C solution, so that the antioxidation of the broad bean pods is increased, and the scratchability of the broad bean pods is improved. The broad bean pods are placed in an antibacterial agent for short-time soaking, so that the humidity of the broad bean pods is improved, the antibacterial agent contains various components which are safe, non-toxic, green and healthy, a protective film can be formed on the surface of each of the broad bean pods, and the scratchability of the broad bean pod is improved. The effects of resisting and restraining bacteria, preventing mould, killing pests, maintaining the storage integrity of the broad bean pods, and preventing the broad beans from changing color, becoming mildewed, or being rotten due to insect bites are achieved.
Owner:蚌埠市涂山绿园蔬菜科研专业合作社

Under-forest ecological plantation method for dendrobium officinale

The invention discloses an under-forest ecological plantation method for dendrobium officinale. According to the method, hot galvanizing iron wire meshes are used for making cultivation tanks, filling bark serves as a cultivation medium, dendrobium officinale seedlings of the right age are planted, the appropriate forest land serves as a support, and the cultivation tanks are suspended for cultivating dendrobium officinale, so that large-scale dendrobium officinale under-forest cultivation is achieved. By means of the method, new choice for cultivating dendrobium officinale is provided, compared with other cultivation methods, the method has the advantages of being easy to operate, low in plantation cost, ecologically-environmentally friendly and great in large-scale plantation potential, and popularization of under-forest plantation of dendrobium officinale can be promoted.
Owner:GUANGXI FORESTRY RES INST

Compound fertilizer for preventing and treating fast-growing eucalyptus red leaf dieback and production method for compound fertilizer

The invention relates to compound fertilizer for preventing and treating fast-growing eucalyptus red leaf dieback and a production method for the compound fertilizer. The compound fertilizer consists of the following raw materials in parts by weight: 2-5 parts of sinistrality alpha-terpilenol, 5-10 parts of azoxystrobin wettable powder with the concentration of 25 percent, 5-10 parts of tea seed meal, 15-20 parts of nitrogen, 5-7 parts of phosphorus, 10-13 parts of potassium, 3-8 parts of boron, 1-2 parts of copper, 3-5 parts of Fe, 1-3 parts of zinc, 10-20 parts of packing and 5-60 parts of an organic fertilizer. The production process comprises the following steps: pretreating all the raw materials, and granulating the raw materials through a rotary drum. The compound fertilizer is mainly used for preventing and treating the red leaf dieback of fast-growing eucalyptus; the prevention and treatment effect is better and more efficient in comparison with that of conventional supplementary spraying of boron fertilizer to leaf surfaces; pesticides and fertilizers are integrated, labor is not repeatedly wasted, and the working efficiency is improved.
Owner:广西乐土生物科技有限公司

Road slope strengthening treatment method based on plant stem and root system grouting

The invention belongs to the technical field of road slope strengthening, and relates to a road slope strengthening treatment method based on plant stem and root system grouting. The method comprises the following steps that firstly, gramineae plants with the developed root systems and the powerful growth capability are sown to a slope surface of a road slope; secondly, after the gramineae plants naturally wither, the branches at the upper portions of the gramineae plants are shorn away, and gramineae plant stems are reserved; thirdly, grouting liquid is prepared, the density of the grouting liquid ranges from 1.2 g / cm<3> to 2.0 g / cm<3>, and the grouting liquid is the mixture of epoxy resin and polyamide resin; fourthly, the grouting liquid is injected into the root systems of the gramineae plants through gramineae plant conduits of the gramineae plant stems; and fifthly, the unearthed position of the gramineae plant stems on the ground serves as the circle center, and the grouting liquid is injected in the circle with the radius ranging from 5 cm to 10 cm. The provided road slope strengthening treatment method based on plant stem and root system grouting is low in cost, green, environment-friendly, high in reliability, capable of facilitating curing, high in adhering force and good in mechanical performance.
Owner:HUBEI UNIV OF TECH
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