The invention relates to the technical field of pesticides and discloses a plant and animal-derived green environment-friendly pesticide and a preparation method thereof. The green environment-friendly pesticide is composed of wild plants, edible crops and animal bile, wherein the wild plants comprise Datura stramonium, castor seed and herba hydrocotylis wilfordi, the edible crops comprise king capsicum, garlic, Sichuan pepper and berberine, and the animal bile is fish bile, chicken bile or pig bile. The preparation method comprises the following steps: washing, crushing and squeezing the wild plants, carrying out chemical examination and extraction of alkaloid and screening out moisture impurities; washing and juicing or soaking the edible crops; washing an animal gallbladder and broking the animal gallbladder to obtain bile; and carrying out mixing and uniform stirring to obtain the pesticide. The pesticide prepared in the invention has bactericidal and anti-decay effects and the advantages of reasonable preparation, a cheap price, convenience in usage, capacity of being both sprayed and poured, no residual poison, no pollution, greenness and environment-friendliness and is applicable to poisoning of a variety of insects on a variety of plants like wheat, corn, cotton, fruit trees, vegetable, fruit and flowers and trees.