Pest control method

A technology of pests, two-spotted mites, applied in the field of pest control

Active Publication Date: 2014-03-19
BEIJING DABEINONG BIOTECHNOLOGY CO LTD
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] It has been proved that plants transgenic for Cry1A.105 can resist Lepidoptera pests s

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pest control method
  • Pest control method
  • Pest control method

Examples

Experimental program
Comparison scheme
Effect test

no. 1 example

[0083] The first embodiment, the acquisition and synthesis of Cry1A.105 gene

[0084] 1. Obtain the nucleotide sequence of Cry1A.105

[0085] The amino acid sequence (1177 amino acids) of the Cry1A.105 insecticidal protein, as shown in SEQ ID NO: 1 in the sequence listing; Cry1A.105 encoding the amino acid sequence (1177 amino acids) corresponding to the Cry1A.105 insecticidal protein Nucleotide sequence (3534 nucleotides), as shown in SEQ ID NO:2 in the sequence listing.

[0086] 2. Obtain the nucleotide sequences of Cry2Ab and Vip3A

[0087] The Cry2Ab nucleotide sequence (1905 nucleotides) encoding the amino acid sequence (634 amino acids) of the Cry2Ab insecticidal protein, as shown in SEQ ID NO: 3 in the sequence listing; the amino acid sequence (789 nucleotides) encoding the Vip3A insecticidal protein amino acid) Vip3A nucleotide sequence (2370 nucleotides), as shown in SEQ ID NO:4 in the sequence listing.

[0088] 3. Synthesize the above nucleotide sequence

[0089]...

no. 2 example

[0090] The second embodiment, construction of recombinant expression vector and transformation of recombinant expression vector into Agrobacterium

[0091] 1. Construction of a recombinant cloning vector containing the Cry1A.105 gene

[0092] The synthesized Cry1A.105 nucleotide sequence was connected to the cloning vector pGEM-T (Promega, Madison, USA, CAT: A3600), and the operation steps were carried out according to the instructions of the pGEM-T vector produced by Promega Company to obtain the recombinant cloning vector DBN01-T , its construction process is as follows figure 1 Shown (wherein, Amp represents the ampicillin resistance gene; f1 represents the replication origin of phage f1; LacZ is the LacZ start codon; SP6 is the promoter of SP6 RNA polymerase; T7 is the promoter of T7 RNA polymerase; Cry1A.105 is the promoter of Cry1A .105 nucleotide sequence (SEQ ID NO: 2); MCS is the multiple cloning site).

[0093] Then, the recombinant cloning vector DBN01-T was trans...

no. 3 example

[0104] The third embodiment, the acquisition and verification of corn plants transferred to the Cry1A.105 gene

[0105] 1. Obtaining corn plants transferred to the Cry1A.105 gene

[0106] According to the commonly used Agrobacterium infection method, the immature embryos of the aseptically cultured maize variety Zong 31 (Z31) were co-cultured with the Agrobacterium described in 3 in the second example, so that the T-DNA in recombinant expression vectors DBN100032, DBN100076 and DBN100029 (including the promoter sequence of maize Ubiquitin gene, Cry1A.105 nucleotide sequence, Cry2Ab nucleotide sequence, Vip3A nucleotide sequence, PMI gene and Nos terminator sequence ) into the maize genome, and obtained the maize plant with the Cry1A.105 nucleotide sequence, the maize plant with the Cry1A.105-Cry2Ab nucleotide sequence and the Cry1A.105-Vip3A nucleotide sequence maize plants; meanwhile, wild-type maize plants were used as controls.

[0107] For Agrobacterium-mediated transfor...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for controlling Athetis lepigone pests. The method includes contacting Athetis lepigone pests with Cry1A.105 protein. According to the invention, the Athetis lepigone pests are controlled by the Cry1A.105 protein that is generated in plants and can kill Athetis lepigone. Compared with the agricultural control method and chemical control method employed in the prior art, the method provided by the invention provides whole growth period and whole plant protection to plants so as to prevent and control disoperation of Athetis lepigone pests. Being simple, convenient and economical, the method provided by the invention has no pollution, no residue, as well as stable and thorough effects.

Description

technical field [0001] The invention relates to a method for controlling pests, in particular to a method for controlling plant damage to plants by using Cry1A.105 protein expressed in plants. Background technique [0002] Athetis lepigone belongs to Lepidoptera Noctuidae and is an omnivorous pest. At present, it is found that it mainly harms corn crops. It is mainly distributed in the summer corn planting areas of Huanghuaihai in my country, and it is mainly distributed in Japan, North Korea, Russia, Europe and other places. The two-spot moth mainly harms the corn roots at the soil surface of the aerial roots of corn, and bites off the corn stems or superficial roots of the corn. A large area of ​​blank land appears, seriously endangering plots and even destroying species. [0003] Corn is the main food crop in China. On July 9, 2011, CCTV News Network reported for the first time the occurrence of Noctuid moth in China. From the autumn of 2011 to May 31, 2012, the National...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N47/44C12N15/84A01H5/00A01P7/04
Inventor 杨旭韩超李胜兵张欣馨张爱红
Owner BEIJING DABEINONG BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap