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50 results about "Athetis lepigone" patented technology

PCR (Polymerase Chain Reaction) primer and identification method of athetis lepigone and sibling species thereof

InactiveCN103266174ASolve the two-point committee mothSolving the puzzle of its morphologically similar speciesMicrobiological testing/measurementDNA/RNA fragmentationEnzyme digestionGenomic DNA
The invention relates to a PCR (Polymerase Chain Reaction) primer and an identification method of an athetis lepigone and a sibling species thereof. The identification method comprises the following steps of: (1) extracting the genomic DNA of the athetis lepigone and the sibling species thereof; (2) carrying out PCR amplification on the mitochondrion COI (Cytochrome Oxidase I) gene of the genomic DNA by taking the genomic DNA of the athetis lepigone and the sibling species thereof as a template; (3) carrying out enzyme digestion on a PCR product prepared from the step (2) by using a restriction enzyme RsaI to obtain an enzyme-digested product; and (4) carrying out agarose gel electrophoresis analysis on the enzyme-digested product prepared from the step (3). According to the invention, the restriction enzyme is a common restriction enzyme, a simple, convenient and stable enzyme digestion marker is provided for screening the athetis lepigone and the sibling species thereof, and an identification technology of the athetis lepigone and the sibling species thereof is explored and established, so that the foundation is established for the future identification, population dynamic monitoring and integrated prevention and control of the athetis lepigone and the sibling species thereof.
Owner:QINGDAO AGRI UNIV
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