Method for identifying molecule of athetis lepigone

A two-point identification technology of Spodoptera frugiperda and molecular identification, applied in the field of DNA sequence identification of insect species, can solve the problems of insufficient understanding of pests, mistaken for cutworms, and delays.

Inactive Publication Date: 2012-07-25
GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
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Problems solved by technology

[0004] As the two spot moth is a new pest that has only attracted people's attention in recent years, the understanding of this pest is not enough. In places where the occurrence is relatively light, people will not pay attention to observation, they will think it is a cutworm, and they will not realize it at all. This is a new pest, and in severe areas, agric...

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  • Method for identifying molecule of athetis lepigone
  • Method for identifying molecule of athetis lepigone
  • Method for identifying molecule of athetis lepigone

Examples

Experimental program
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Embodiment 1

[0028] 1. Collecting Insect Samples

[0029] In mid-to-early August, during the blooming period of the spodopteryx moth, adults were lured by lamps in 13 cities and counties in Hebei, Henan, Shandong, and Shanxi provinces. The typical characteristics of the adults were used to ensure that the sampled samples were spodoptera sp., and then Store in 95% ethanol at -20°C.

[0030] Table 1 The collection information of the population samples of Noctuid moth in the Second Committee

[0031] Table 1 Collecting data and individual number of Athelis lepigone

[0032]

[0033] 2. Extraction of Genomic DNA

[0034] Insect genomic DNA was extracted according to the Animal Tissue / Cell Genome Rapid Extraction Kit (SK8252) of Shanghai Sangon Bioengineering Co., Ltd., and stored at -20°C for later use.

[0035] 3. Amplification, cloning, sequencing and sequence analysis of COI gene

[0036] (1) Amplification of COI gene

[0037] Primers for PCR amplification of COI gene sequenc...

Embodiment 2

[0069] 1. Collecting Insect Samples

[0070] At the end of August, adults were lured by lamps in six cities and counties in Hebei Province. After being identified by experts according to the characteristics of the adults, they were placed in 95% ethanol and stored at -20°C.

[0071] Table 4 Sample collection information

[0072] Table 4 Collecting data of insects

[0073]

[0074] 2. Extraction of Genomic DNA

[0075] Insect genomic DNA was extracted according to the Animal Tissue / Cell Genome Rapid Extraction Kit (SK8252) of Shanghai Sangon Bioengineering Co., Ltd., and stored at -20°C for later use.

[0076] Amplification, cloning, sequencing and sequence analysis of COI gene

[0077] (1) Amplification of COI gene

[0078] Primers for PCR amplification of COI gene sequence: upstream primer / downstream primer 5-GGTCAACAAATCATAAAGATATTGG-3 / 5-TAAACTTGAGGGTGACCAAAAAATCA-3

[0079] 20 μL PCR reaction system: The total PCR amplification reaction system is 20 μL, i...

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Abstract

The invention relates to a deoxyribose nucleic acid (DNA) sequence for identifying the type of an insect, in particular to the DNA sequence of new corn insect athetis lepigone and a method for identifying a molecule of the athetis lepigone. The invention provides the DNA sequence of a cytochrome oxidase (COI) gene of the athetis lepigone. The DNA sequence comprises the sequence shown as SEQ ID No: 1 or the sequence with at least 99 percent of sequence identity of the sequence shown as SEQ ID No: 1. The DNA sequence and the method have the effect that whether an insect is the athetis lepigone or not can be quickly and accurately judged by comparing the COI gene sequence of the detected insect and the sequence provided by the invention, and a basis for taking a corresponding efficient control measure in a field is provided.

Description

technical field [0001] The invention relates to a DNA sequence for identification of insect species, in particular to the DNA sequence of the new corn pest Spodoptera spp. and its molecular identification method. Background technique [0002] Two o'clock moth[ Athetis lepigone (M?schler, 1860)] is a new agricultural pest in my country's summer corn production areas. [0003] According to relevant records, in July 2005, it was first discovered in Shijiazhuang, Hebei Province in China that the two-spot moth damages summer corn seedlings, bores the base of the stem, causes the seedlings to wilt and die, or bites off the corn roots, leading to lodging, causing Serious lack of seedlings and monopoly, this is the first report in China. According to reports, in 2010, Noctuid moths commonly occurred in the central and southern summer corn areas of Hebei Province. In 2011, they broke out in Hebei, Henan, Shandong, Shanxi, Anhui, Jiangsu and other provinces, with an area of ​​more t...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 朱彦彬李志勇白辉董立马继芳李立涛刘磊董志平
Owner GRAIN RES INST HEBEI ACAD OF AGRI & FORESTRY SCI
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