1256 results about "Deoxyribose" patented technology

The invention discloses a DNA (Deoxyribose Nucleic Acid) sequencer which comprises a supporting table, a plurality of vibration dampers, a vibration damping plate, a reaction bin assembly, a CCD (Charge Coupled Device) camera, a two-dimensional regulation supporting device and a medicament supply assembly, wherein the vibration damping plate is connected with the supporting table by a plurality of vibration dampers; the reaction bin assembly is fixedly arranged on the vibration damping plate and is used for performing the DNA sequencing reaction; the CCD camera is used for acquiring an optical signal; the two-dimensional regulation supporting device is used for supporting the CCD camera; and the medicament supply assembly is arranged on the supporting table and is used for providing reagents and buffer solution for the reaction bin assembly. According to the DNA sequencer disclosed by the invention, by the arrangement of a plurality of reaction bins and the matching of the two-dimensional regulation supporting device capable of carrying out two-dimensional regulation and the medicament supply assembly capable of supplying the reagents for a plurality of reaction bins, the aim of simultaneously performing a plurality of reactions is fulfilled, a plurality of samples can be simultaneously sequenced and the DNA sequencing efficiency is greatly improved.

This invention provides a nucleotide analogue comprising (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine and uracil, (ii) a deoxyribose, (iii) an allyl moiety bound to the 3′-oxygen of the deoxyribose and (iv) a fluorophore bound to the base via an allyl linker, and methods of nucleic acid sequencing employing the nucleotide analogue.

The present invention relates to a nutritional composition containing protein, fat and carbohydrate; and a. a nucleotide component selected from the group consisting of nucleic acid, nucleic acid derivatives, nucleotides, nucleoside polyphosphates, polynucleotides, nucleosides, ribose, desoxyribose, and dinucleosidpolyphosphates (Np_xN); and b. a non-proteinaceous negatively charged, glycan or glycoconjugate component with a molecular weight between 200 and 20.000 dalton. The present nutritional composition is particularly suited for feeding infants as it mimics the protective effects of human milk, in particular against allergies and infections.

The invention discloses preparation of an ECL (electro chemical luminescence) DNA (Deoxyribose Nucleic Acid) sensor based on a 3D paper chip, and application of the sensor to simultaneous detection on Hg<2+> and Ag<+>. The preparation method of the sensor (the schematic diagram of which is shown in the figure) comprises the following steps of: designing a micro-fluidic chip pattern on a computer, and preparing a 3D paper chip sensor; preparing nano materials such as a porous gold nanowire, PdAg alloy, a carbon point and P acid according to existing methods, preparing PdAg@CQDs and PdAg@P-acid with signal amplification functions as ECL probes, and respectively compounding the ECL probes with DNA subjected to specific binding with Hg<2+> and Ag<+>; and modifying the porous gold nanowire to the surface of an electrode of the sensor by an electrode surface modifying technology, and absorbing a DNA segment to prepare the DNA sensor. The method for simultaneously detecting Hg<2+> and Ag<+> comprises the following steps of: connecting the modified electrode to an ECL apparatus, and detecting Hg<2+> and Ag<+> in a sample extracting solution. The electrode of the sensor is strong in specificity and high in sensitivity, and is capable of reaching a p mol level. When the paper chip sensor easily processed is used for simultaneously detecting two materials on the same electrode, the detection efficiency is improved, and the cost is lowered.

A novel nucleoside analog is disclosed which comprises a piperazine ring in the place of the ring ribose or deoxyribose sugar. Monomers utilizing a broad variety of nucleobases are disclosed, as well as oligomers comprising the monomers disclosed herein linked by a variety of linkages, including amide, phosphonamide, and sulfonamide linkages. A method of synthesizing the nucleoside analogs is also disclosed.

A nucleic acid, which is provided in a large amount through a nucleic acid amplification reaction with the use of chimeric oligonucleotide primers, is constructed in a state of containing a modified deoxyribonucleotide for immobilizing the nucleic acid to a solid phase and then immobilized to a solid phase at a high efficiency, thereby giving an immobilized nucleic acid product with excellent qualities.

The invention discloses a method for preparing a meso-porous silicon nano medicine carrier with cell specificity target, reduction responsiveness and triple anticancer treatment effects. The method comprises the following steps: firstly, synthesizing meso-porous silicon nano particles by using a gel dissolution method, subsequently, introducing a disulfide bond onto the surface of a meso-porous silicon nano reservoir by using a chemical modification method, innovatively fixing cytochrome C with an apoptosis-inducing function onto the surface of the meso-porous silicon nano reservoir, blocking meso-porous channels with medicines, finally modifying DNA (Deoxyribose Nucleic Acid) aptamer single chain molecules (AS1411, with a cancer cell apoptosis-inducing function) onto the surface of a meso-porous silicon/cytochrome C nano composite system, and taking the system as specificity ligand of a receptor (nucleolin protein) which is overexpressed on the surface of liver cancer cytomembrane, thereby establishing a multifunctional composite type nano medicine carrier system for achieving triple anticancer treatment under combined action of medicines, blocking substances and target molecules inside meso-pores.

The invention discloses a gene-specific molecular marker Pi2SNP of a rice blast-resistant gene Pi2 as well as a preparation method and application thereof. The molecular marker Pi2SNP is a nucleotide fragment II in a specific banding pattern with the rice blast-resistant gene Pi2 obtained by amplifying F1 and R1 from the total DNA (Deoxyribose Nucleic Acid) of the rice blast-resistant variety carrying rice blast-resistant gene Pi2 by use of a primer to obtain a nucleotide fragment I and then performing enzyme digestion of the nucleotide fragment I by use of restriction endonuclease Pst I or Hinf I. The molecular marker is the first Pi2 gene-specific SNP marker developed for the sequence in the Pi2 gene, and has the advantages of high specificity and low cost and high flux in practical application, and can be widely applied to the colonies with different inheritance backgrounds. By adopting the molecular marker, the utilization efficiency of the gene in molecular marker-assisted selective breeding, gene pyramiding breeding and transgenic breeding can be improved.

The invention provides an electrochemical miRNA (micro Ribose Nucleic Acid) detection method based on a DNA (Deoxyribose Nucleic Acid) three-dimensional nano structure probe. The electrochemical miRNA detection method comprises the steps of: synthesizing a DNA three-dimensional nano structure probe through a self-assembly method, wherein the DNA three-dimensional nano structure probe comprises one section of extended recognition sequence; assembling the DNA three-dimensional nano structure probe on the surface of a working electrode of an electrochemical device; hybridizing a target miRNA with the DNA three-dimensional nano structure probe on the surface of the working electrode; and adding oxidordeuctase and a corresponding substrate, and carrying out electrochemical detection by using the electrochemical device. The method can be used for detecting the miRNA of 10aM, therefore, the problem of the great demand on test samples in the detection method in the prior art is solved. In addition, the method has strong specificity selection and can be well used for distinguishing base pair mismatching of same family of miRNA. Compared with the method using the single-chain DNA probe, the electrochemical miRNA detection method is higher in stability.

The invention relates to a method for preparing Decitabine. The particular proposal for solving the technical problem is as follows: 2-deoxidtion-D-ribose, 10 percent of HCL methanol solution, methoxyacetic acetic anhydride, HMDS, acetic anhydride, tri-silicyl tri-fluorine methane sulfonic acid ester, acetic acid amine, etc. are adopted as raw materials to synthesize the Decitabine; the target product of the Decitabine is obtained through the five steps of reactions, namely, methylation, acylation, trimethyl silication, ammoniation and deacylation with a total yield of above 18.4 percent and a product purity of above 99.7 percent.

The invention relates to the technical field of molecular biology, and discloses a method for preparing a genome mixed-sample sequencing library, which comprises the following steps: (1) ultrasonic fragmentation of genomic DNA; (2) purification and end repair of fragmented products; (3) ) repair product for purification and adapter ligation; (4) ligation product purification recovery and concentration determination; (5) sample mixing and fragment screening; (6) PCR amplification of the product after fragment screening; (7) purification of the PCR product to obtain Sequencing library; (8) library quality inspection and on-machine sequencing. The present invention provides an adapter compatible with the Illumina next-generation sequencer in the above step (2), a method for purifying the product in the step (2) (3) (4) (7), and a method for mixing samples in the step (4) And the PCR amplification system and program setting in the step (6) ensure that the library building method provided by the present invention can quickly and smoothly carry out multiple sample mixed sample library building, and the method provided by the present invention can be used to obtain good data uniformity. and high-quality sequencing data.

The invention discloses a test strip for detecting aflatoxin B1 or M1 by utilizing an aptamer. The test strip for detecting the aflatoxin B1 or M1 by utilizing the aptamer, provided by the invention, comprises a sample absorption pad, a marker pad, a reaction film and a water absorption pad, wherein the marker pad is coated with a detection probe; the detection probe is an aflatoxin B1 aptamer marked by fluorescein; the nucleotide sequence of the aflatoxin B1 aptamer is sequence 1; the reaction film comprises a detection region and a quality control region; the detection region is coated with a conjugate formed by an aflatoxin B1 hapten and a carrier protein; and the quality control region is coated with a quality control probe, and the quality control probe is a conjugate formed by avidin conjugation probe marked aflatoxin B1 complementary single strand DNA (Deoxyribose Nucleic Acid)) molecules. The test strip for detecting the aflatoxin B1 or M1, provided by the invention, has the advantages of high sensitivity, accuracy in quantifying, strong specificity, simplicity and convenience, and short detection time.

The invention discloses a method for detecting a surviving gene based on a graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor. The method comprises the following steps: a specific probe is designed according to a gene segment to be detected; a capture probe is self-assembled on the surface of G-3DAu/GCE through a gold-sulfur bond; the capture probe and a signal probe with the tail end marked by biotin are respectively combined with a target DNA to form a 'sandwich' model in the presence of the target DNA; a horse radish peroxidase marked by avidin can be combined with the biotin marked by the signal probe, so that the HRP (Horse Radish Peroxidase) can be fixed on the surface of an electrode; the electrode is placed in a base solution of 3, 3', 5, 5'-tetramethyl benzidine (TMB) and H2O2, and the H2O2 can oxidize TMB to generate a bidiazotizedbenzidine material under the catalyzing of the HRP, so that an electrochemical signal is generated. The method has the advantages of being simple, quick and green in preparation process, and high in selectivity and sensitivity.

The present invention provides an improved method for cDNA preparation. The method of the present invention comprises the following steps: (1) contacting mRNA with a cDNA synthesis primer which can anneal to RNA and a suitable enzyme which possesses reverse transcriptase activity under conditions sufficient to permit the template-dependent extension of the primer to generate an mRNA-cDNA intermediates; (2) contacting a mixture from step 1 with a deoxyribonucleotide adapter in the presence of Mn²⁺-ions, wherein said oligonucleotide adapter has a pre-selected arbitrary nucleotide sequence at its 5′-end, and a short dG stretch at its 3′-end. The 3′-end nucleotide of the adapter is a terminator nucleotide, e.g., a nucleotide with a modified 3′-OH group of a deoxyribose residue.

The invention provides asymmetric deoxyribose nucleic acid (DNA) artificial adapters by using a second-generation high-throughput sequencing technology, which is composed of two DNA oligonucleotide single strands. The preparation method comprises the following steps: dissolving the two DNA oligonucleotide single strands into a quenching solution; regulating the final concentration to 2mM and volume to 20microlitre; carrying out a quenching reaction to form the DNA artificial adapters which are locally and mutually complemented at a temperature of 95 DEG C for 5 minutes; reducing the temperature of 95 DEG C to 12 DEG C at the speed of 0.1 DEG C per second; keeping the temperature of 12 DEG C, and diluting an adapter solution after quenching to 500 mu M at a ratio of 1:4; and storing at the temperature of minus 20 DEG C. An application of the asymmetric DNA artificial adapters by using the second-generation high-throughput sequencing technology is as follows: A, asymmetric DNA adapters are in ligation with DNA samples; B, non-connected DNA artificial adapters are purified and isolated by using electrophoresis tapping; C, judgment is carried out; and D, a polymerase chain reaction (PCR) amplification reaction is performed based on an asymmetric sequence. The asymmetric DNA artificial adapters by using the second-generation high-throughput sequencing technology has the obvious advantages that: 1) the usage of original DNA samples for establishing a library is reduced to 50 nanogram and the sensitivity of the original DNA samples is improved by 100 times; and 2) 100% of effective sequencing samples are produced in the process of adapter connection for establishing the library.

The invention relates to a separated deoxyribose nucleic acid (DNA) sequence. The DNA sequence comprises a nucleotide sequence (a) of SEQIDNO.1 or SEQIDNO.2; or a nucleotide sequence (b) complementary with the nucleotide sequence (a). The invention further provides a recombinant expression vector containing the nucleotide sequence, a genetic engineering host cell and application of the nucleotide sequence, the recombinant expression vector and the host cell for encoding the diacylglycerol acyltransferase or producing triacylglycerol. The DNA sequence has the advantages that a cDNA full-length sequence and a DNA full-length sequence of a parietchloris incise diacylglycerol acyltransferase gene are obtained by screening, encoded protein of the gene has a triacylglycerol synthesis capability by expression of the gene in a TAG synthesis defect strain H1246 of yeast, and conditions are created for utilizing the gene to achieve large-scale synthesis of triacylglycerol by genetic operation.

The invention discloses a molecule marking method of a rice blast-resisting gene, belonging to the field of crop molecule heredity and breeding. The method comprises the following steps: (1) taking a rice sample and extracting a genome DNA (Deoxyribose Nucleic Acid) of the rice sample; and (2) carrying out PCR (Polymerase Chain Reaction) amplification on the genome DNA of the rice sample by utilizing any one pair of molecule-marked primers in RM6091 and RM26632, carrying out electrophoresis detection on a PCR amplification product, and if a molecule-marked DNA segment with corresponding size is amplified, showing that a Pi-bdl(t) gene exists. Through rice blast-resisting gene Pi-bdl(t) molecule marking in the invention, whether thin rice as well as crossbred descendants, backcross descendants and multiple cross descendants thereof contain the gene can be detected, the resistance level of the gene on rice blast can be forecasted, the selecting efficiency of rice blast resistant materials can be greatly increased, and the breeding process for disease resistance can be accelerated.

Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin. In preferred embodiments, constructs further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, host cells comprising constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e.g. thymidine is also disclosed.

The invention relates to an establishment method of a long mate pair library. The establishment method comprises the steps as follows: 1) randomly interrupting DNA (deoxyribose nucleic acid), and completing and repairing the gaps at the tail end; 2) preparing A-Fragment; 3) carrying out cohesive end connection to A-Fragment and LMP Adaptor; 4) digesting the LMP-Adaptor-Fragment with at least two restriction enzymes which can distinguish four basic groups; 5) cyclizing the fragments in the obtained enzyme digesting library again; and 6) amplifying the purified ring molecule through primer of the LMP Adaptor; and recovering the amplified products, thus finishing the establishment of LMP (long mate pair) library. According to the library establishment method provided by the invention, only two 63bp oligonucleotides sequences are needed to be synthetized, and simple experiments such as molecular biology connection and amplification are carried out, and the two ends of the obtained LMP library have the sequencing primer sequences which can be directly applied to next generation sequencing; and moreover, the fragment subjected to secondary cyclizing has the known restriction enzyme site, and the sequences of two ends of the to-be-tested fragments can be quickly sorted, the sequence testing data can be effectively sieved, and the mosaic sequence can be removed.