24 results about "Deoxyribose" patented technology

The application discloses a KASP marker related to pea powdery mildew resistance and application thereof. The application also discloses a SNP site related to pea powdery mildew resistance, which is the 583th deoxyribonucleotide of the DNA molecule described in sequence 4 in the sequence listing in the pea genome, and the polymorphism is T / -; the application further discloses a KASP marker developed based on the SNP site for identifying the pea powdery mildew resistance. Experiments prove that the KASP marker can be effectively applied to identification of pea powdery mildew resistance resources and molecular marker assisted breeding of a pea powdery mildew resistance offspring population, and has important value in pea production, breeding work and research on disease resistance mechanism.

The present application relates to the technical field of cosmetic biotechnology and nano-carrier drug delivery, and provides PDRN-putrescine co-delivery nano-liposome as well as a preparation method and application thereof.The PDRN-putrescine co-delivery nano-liposome provided by the present application comprises the following components in mass fraction: 0.1-3% of polydeoxyribonucleotide; 0.1-3% of putrescine; 2-8% of phospholipid emulsifier; 0-15% of polyhydric alcohol cosolvent; and the balance is a buffer solution; and the buffer solution has a pH value of 6-7.5.The present application realizes stable co-encapsulation of PDRN and putrescine by constructing a specific buffer system to regulate the pH value, using the amphiphilic property of the phospholipid bilayer and the in-situ ion pair technology, solves the compatibility problem of PDRN and putrescine, significantly improves the transdermal absorption rate of PDRN through transmembrane delivery of the nano-carrier, and realizes the synergistic repair and anti-aging effect of PDRN and putrescine at the cellular level.

The application belongs to the technical field of sequencing-by-synthesis chip, and particularly relates to a three-dimensional porous array enhanced SBS sequencing chip and a preparation method thereof, which comprises a silicon wafer as a substrate and a light-controlled reversible terminator reaction system; the silicon wafer surface has a mesoporous film, and the mesoporous film has reaction cavities and a closed microchannel network; the inner wall of the mesoporous film is grafted with mercaptopropionic acid and covalently coupled with an amino-modified probe; the reaction cavity bottom is etched with a cylindrical array; the light-controlled reversible terminator reaction system comprises deoxyribonucleotide triphosphates, the 3'-OH end of the deoxyribonucleotide triphosphates is introduced with a protective group, and the 5' end amino group of the deoxyribonucleotide triphosphates is specifically combined with a fluorescent group; and the application effectively breaks through the technical bottlenecks of the existing NGS sequencing chip in terms of throughput, accuracy, cost and adaptability of complex samples.

The application discloses a SNP marker related to cold resistance on the short arm of wheat 2A chromosome and application thereof, and belongs to the field of biotechnology and molecular breeding. In order to identify, assist in identifying and / or compare the cold resistance of wheat, the application provides application of a SNP molecular marker, wherein the SNP molecular marker is a deoxyribonucleotide at position 361 of SEQ ID NO:1, which is G or A, and the application can be application of the SNP molecular marker in identifying or assisting in identifying the cold resistance of wheat. By using whole genome association analysis, the application identifies that a SNP at position 361 of the nucleotide sequence of SEQ ID NO.1 is significantly related to the cold resistance of wheat, the SNP molecular marker is not limited by the growth stage of wheat, and selection can be performed at the seedling stage, so that the selection period of wheat breeding is significantly shortened, the breeding efficiency is improved, and the SNP molecular marker assisted breeding for improving the cold resistance of wheat can be used in practice.

The present application provides a hypoxic microenvironment-responsive artificial deoxyribonucleic acid and application. Specifically, the present application provides a compound of formula (I), or an optical isomer, hydrate, solvate thereof, or a pharmaceutically acceptable salt thereof. The compound of the present application can be used as a molecular probe responding to hypoxic microenvironment stress, has superior microenvironment targeting and biological stability, and can be used for diagnosis and treatment of cancer or tumor (especially solid tumor) characterized by hypoxic microenvironment.

This invention belongs to the field of agricultural biotechnology and discloses a low-temperature synergistic biological agent, its preparation method, and its application. The core innovation lies in using a unique engineered Bacillus subtilis strain as the core functional carrier, and performing a ternary targeted compounding with polydeoxyribonucleotides (PDRN) and biostimulants to achieve a triple synergistic low-temperature resistance effect. The engineered Bacillus subtilis strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) on March 11, 2026, with accession number CGMCC No. 37899. The chassis strain of Bacillus subtilis was deposited at the same center on March 3, 2026, with accession number CGMCC No. 37834. The modification strategy involves knocking out the α-subunit gene of acetoin dehydrogenase and the phosphoacetyltransferase gene from the genome of Bacillus subtilis CGMCC No. 37834, and expressing the acetyllactate synthase gene and the α-acetyllactate decarboxylase gene.

This invention relates to the field of biotechnology, specifically to a method and application for the co-production of plant vesicles and plant polydeoxyribonucleotides. This invention achieves the goal of synergistically extracting two high-value-added products from the same raw material, significantly improving the comprehensive utilization rate of the raw material. The plant vesicles obtained by this invention have an average particle size of 50-200 nm and a concentration as high as 8.7 × 10⁻⁶. 11 The plant polydeoxyribonucleotides have high purity and smaller molecular weight, with a molecular weight of <100 bp.

The application discloses a research method for interaction between a new pollutant and DNA, and the method comprises the following steps: using a DFT method in quantum chemistry calculation software to optimize the molecular structure of the new pollutant and deoxy nucleoside; using Multiwfn software to obtain the contribution of each atom to a molecular orbital and atomic charge distribution; finding an electrophilic site of the new pollutant and a nucleophilic site of the deoxy nucleoside; using molecular docking software to perform docking calculation on the new pollutant and a double-stranded DNA fragment and search for an optimal combination position; using a UV-Vis ultraviolet spectrophotometer to perform ultraviolet spectrum titration on the interaction between the new pollutant and the double-stranded DNA; using a super high performance liquid chromatography-mass spectrometry method to perform gradient elution, detecting the deoxy nucleoside through a multiple reaction detection method, calculating a reaction rate, and identifying a deoxyribose unit prone to adduct reaction with the new pollutant; and analyzing the detection result.

This invention relates to a method for producing polydeoxyribonucleotide (PDRN) from plants, the PDRN produced by said method, and skin-improving cosmetic compositions, pharmaceutical compositions, quasi-pharmaceutical compositions, food compositions, health functional foods, and feed compositions containing said PDRN as an active ingredient. Through this invention, high-purity and high-concentration PDRN can be produced from plants. Furthermore, because the plant-derived PDRN produced in this way has skin protection against ultraviolet radiation, skin barrier strengthening, moisturizing, wrinkle improvement, skin regeneration, wound healing, or anti-aging effects, it can be effectively used as a skin-improving cosmetic composition, pharmaceutical composition, food composition, health functional food, quasi-pharmaceutical composition, and feed composition.

This invention provides a cosmetic composition with anti-wrinkle and repairing effects, its preparation method, and its application. The cosmetic composition with anti-wrinkle and repairing effects includes polydeoxyribonucleotides, fibronectin, and serum proteins. In the composition of this invention, polydeoxyribonucleotides can rapidly activate cell activity, while fibronectin and serum proteins, as core nutrients, can quickly enter the skin using the already repaired skin's metabolic capacity. The combination of these three components has a synergistic effect, which can significantly improve the skin's wrinkle-reducing and repairing effects.

The present disclosure provides a single-stranded oligonucleotide, wherein the single-stranded oligonucleotide has a length of 16-30 nucleotides and can inhibit the expression of APOE4 mRNA by the mechanism of RNA interference (RNAi); wherein each nucleotide in the single- stranded oligonucleotide independently of one another is a modified or unmodified nucleotide; and wherein at least one nucleotide is a nucleotide X, and at least one nucleotide is a fluoro modified nucleotide; and in a 5' to 3' direction, in the single-stranded oligonucleotide, the 13th nucleotide is a substituted alkoxy modified nucleotide; the 14th nucleotide is a nucleotide X; and each of the 15th nucleotide and all the subsequent nucleotides independently of one another is a modified nucleotide; and each nucleotide X is independently a deoxyribonucleotide or an unmodified nucleotide. The present disclosure also provides a double-stranded oligonucleotide, an oligonucleotide conjugate and a pharmaceutical composition comprising the single-stranded oligonucleotide as an antisense strand.

This invention belongs to the field of biopharmaceutical manufacturing technology and discloses an antioxidant and anti-aging PN complex and its preparation method, comprising: polydeoxyribonucleotide, epigallocatechin gallate, divalent magnesium ions, sodium ascorbate phosphate, citrate-sodium citrate buffer system, and water; the divalent magnesium ions form chelates with the phosphate groups on the polydeoxyribonucleotide molecular chain and the phenolic hydroxyl groups of epigallocatechin gallate, respectively, so that epigallocatechin gallate forms a continuously distributed molecular coating layer on the surface of the polydeoxyribonucleotide molecular chain; this invention solves the problem of heterogeneous gel aggregation caused by excessively fast reaction kinetics in the preparation of biopharmaceutical macromolecules by transforming the unavoidable disordered crosslinking between chains in conventional mixing processes into highly ordered intramolecular homogeneous encapsulation.

This invention relates to a method for detecting target nucleic acids based on one-pot isothermal nucleic acid amplification. The invention provides a method for detecting target nucleic acids in a sample, comprising the following steps: a) placing a reaction component and a sample containing the target nucleic acid in the same reaction vessel for amplification reaction, wherein the reaction component comprises i) a single-stranded DNA padlock probe, comprising a first linker region at the 5' end, a second linker region at the 3' end, and an internal reporter region, wherein the first and second linker regions each contain a polynucleotide sequence complementary to the target nucleic acid sequence; optionally, the single-stranded DNA padlock probe is composed entirely of deoxyribonucleotides, or substantially composed of deoxyribonucleotides and containing no more than four consecutive ribonucleotides; ii) a ligase or a variant thereof; iii) a DNA polymerase or a variant thereof with strand displacement activity; and iv) a reaction buffer; b) detecting the amplification reaction product of step a). The invention also provides a kit suitable for the above method.

The application discloses a method for regulating catalytic behavior of 3'->5' exonuclease based on deoxyribose configuration adjustment and application thereof, and belongs to the technical field of nucleic acid chemistry, biological catalytic regulation and molecular engineering. In view of the problems of poor position accuracy and unpredictable effect of the existing nucleic acid enzyme regulation method, a hydrazine compound containing an NH-NH2 functional group is used to modify and reduce an AP site in DNA, so that the deoxyribose of the AP site is changed from a closed ring configuration to a stable open chain configuration. The DNA after the AP site is modified is incubated with a nucleic acid enzyme with 3'->5' exonuclease activity, so that the nucleic acid enzyme is specifically catalyzed to stop at a nucleotide downstream of the modified AP site, and the catalytic behavior of the nucleic acid enzyme is accurately and controllably regulated with single nucleotide resolution. The application does not need to rely on steric hindrance or enzyme molecular structure modification, the regulation effect is stable and predictable, and the application can be widely applied to high-resolution DNA damage positioning, controllable DNA assembly and DNA oxidative damage level detection.

The present disclosure provides a single-stranded oligonucleotide, wherein the single-stranded oligonucleotide has a length of 16-30 nucleotides and can inhibit the expression of PCSK9 mRNA by the mechanism of RNA interference (RNAi); wherein each nucleotide in the single- stranded oligonucleotide independently of one another is a modified or unmodified nucleotide; and wherein in the single-stranded oligonucleotide, at least one nucleotide is a nucleotide X, and at least one nucleotide is a fluoro modified nucleotide; and in a 5' to 3' direction, the 13th nucleotide is a substituted alkoxy modified nucleotide; the 14th nucleotide is a nucleotide X; and each of the 15th nucleotide and all the subsequent nucleotides independently of one another is a modified nucleotide; and each nucleotide X is independently a deoxyribonucleotide or an unmodified nucleotide. The present disclosure also provides a double-stranded oligonucleotide, an oligonucleotide conjugate and a pharmaceutical composition comprising the single-stranded oligonucleotide as an antisense strand.

This invention discloses a PDRN-amino acid composite toothpaste and its preparation method. By weight percentage, the toothpaste comprises a humectant, an abrasive, a foaming agent combination, a sweetener, a thickener, a fragrance, a preservative, a main active ingredient, auxiliary active ingredients, and water. The main active ingredient includes glycine and polydeoxyribonucleotides derived from spirulina. The foaming agent combination comprises lauroyl alanine arginine salt and alkyl glycosides, with a weight ratio of lauroyl alanine arginine salt to alkyl glycosides of 1:3 to 3:1. This invention utilizes small-molecule spirulina PDRN, which is easily absorbed and retained. Simultaneously, the salt formation of arginine and lauroyl alanine solves the micelle dispersion problem. The resulting toothpaste is mild and non-irritating, produces rich foam, and has stable consistency, offering multiple benefits such as repairing oral mucosa, anti-inflammation, antibacterial properties, and anti-sensitivity effects.

The disclosure herein includes methods and compositions for nucleic acid synthesis using terminal deoxynucleotidyl transferase with a plurality of deoxyribonucleotide triphosphates each comprising a modified base having a photocleavable carbon chain moiety, which when present, enables single incorporation.

The application belongs to the technical field of genetic engineering, and particularly relates to specific ginseng-derived nucleic acid molecule sequences and application thereof as PDRN. The ginseng-derived polydeoxyribonucleotide provided by the application is a ginseng-derived nucleic acid molecule with a specific sequence, which is rationally designed and chemically synthesized, and does not need to rely on extraction of natural ginseng tissues, thereby solving the problems of low extraction efficiency and high cost of plant-derived PDRN. Moreover, the PDRN material prepared from the ginseng-derived polydeoxyribonucleotide has low cytotoxicity, can effectively promote and improve skin inflammation, promote vascular formation, and significantly promote collagen synthesis in cells, and has a wide application prospect.

The present invention is directed to provide novel RNA molecules, chimeric NA molecules, double-stranded RNA molecules, and double-stranded chimeric NA molecules. Specifically, an embodiment of the present invention is an RNA molecule for RNA interference to target a mutant allele with a point mutation, in which (1) the molecule has a nucleotide sequence complementary to a nucleotide sequence of a coding region of the mutant allele; and (2) when counted from the base at the 5′-end in a nucleotide sequence complementary to a nucleotide sequence of the mutant allele, (2-1) a base at position 5 or 6 is mismatched to a base in the mutant allele; (2-2) a position 10 or 11 corresponds to the position of the point mutation; and (2-3) a group at the 2′-position of a pentose at positions 6-8 or positions 7 and 8 is modified with, e.g., OCH3. In this RNA molecule, one or more ribonucleotides may be replaced by, e.g., a deoxyribonucleotide. The molecule may form a double-stranded RNA with a complementary strand.

This invention discloses a hydrogel for osteoarthritis repair, its preparation method, and its applications, relating to the field of biomedical technology. The hydrogel is made of polydeoxyribonucleotides and polyglutamic acid, and has a porous structure with an average pore size of 50-200 μm. The invention involves mixing a PDRN solution with a PGA solution, then adding a cross-linking agent solution, stirring until homogeneous, and allowing the mixture to stand to react, thus forming the hydrogel. Cross-linking PDRN with PGA increases the mechanical strength of the hydrogel, and PDRN is slowly released within the hydrogel, exhibiting anti-inflammatory effects and a significant therapeutic effect on osteoarthritis.