11 results about "Immobilized Nucleic Acids" patented technology

The present disclosure provides compositions and related methods, e.g., for preparing immobilized nucleic acid nanostructures using compaction oligonucleotides. In some embodiments, rolling circle amplification reaction can be conducted with compaction oligonucleotides on-support or in-solution to generate concatemer molecules having multiple copies of a polynucleotide unit arranged in tandem. Each polynucleotide unit comprises a sequence-of-interest and at least one universal adaptor sequence that binds one end of a compaction oligonucleotide. The 5′ and 3′ regions of the compaction oligonucleotide can hybridize to the concatemer to pull together distal portions of the concatemer causing compaction of the concatemer to form a nanostructure. Nanostructures having tighter size and shape compared to concatemers generated in the absence of the compaction oligonucleotides. The compact and stable characteristics of the nucleic acid nanostructures improves sequencing accuracy by increasing signal intensity and they retain their shape and size during multiple sequencing cycles.

The invention discloses a fluorescent probe device with high-sensitivity detection in the field of biological fluorescence detection.The fluorescent probe device comprises a micro-fluidic chip, a closed micro-channel allowing a sample to flow is arranged in the micro-fluidic chip, and the micro-channel comprises a reaction bin, a sample bin and an enzyme liquid bin; the sample bin and the enzyme liquid bin are symmetrically distributed on the two sides of the reaction bin, delay channels of zigzag structures are communicated between the sample bin and the reaction bin and between the enzyme liquid bin and the reaction bin, the sample bin and the enzyme liquid bin are provided with a first filling port and a second filling port respectively, the reaction bin is provided with vent holes communicated with the outside, and a plurality of aptamer fluorescent probes are fixed to the bottom wall of the reaction bin. A nucleic acid aptamer fluorescent probe is fixed in the micro-fluidic chip, a CRISPR-Cas12a enzyme digestion system is added, a plurality of signal fragments are released in the process that a single aptamer is combined with antibiotics, signal cascade amplification of CRISPR-Cas12a non-specific enzyme digestion activation is achieved, and the micro-fluidic chip has the function of detecting existence of drug-resistant genes. And the possibility of occurrence of false negative detection results is reduced as much as possible.

The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and / or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.

Examples herein involve amplification and detection of nucleic acids using a microfluidic reaction chamber. An example apparatus includes a reaction-chamber circuit to process a reagent and a biologic sample for amplification of nucleic acids. The apparatus further includes a plurality of capillaries to pass the reagent and the biologic sample through the microfluidic reaction chamber. A valve control system may selectively control each of a plurality of valves to cause the reagent and the biologic sample to selectively move through the microfluidic reaction chamber for the amplification of the nucleic acids according to a particular timing sequence. In various examples, a trapping region disposed in the microfluidic reaction chamber secures the nucleic acids in the microfluidic reaction chamber for amplification using the reaction-chamber circuit.

The present disclosure provides compositions and related methods, e.g., for preparing immobilized nucleic acid nanostructures using compaction oligonucleotides. In some embodiments, rolling circle amplification reaction can be conducted with compaction oligonucleotides on-support or in-solution to generate concatemer molecules having multiple copies of a polynucleotide unit arranged in tandem. Each polynucleotide unit comprises a sequence-of-interest and at least one universal adaptor sequence that binds one end of a compaction oligonucleotide. The 5′ and 3′ regions of the compaction oligonucleotide can hybridize to the concatemer to pull together distal portions of the concatemer causing compaction of the concatemer to form a nanostructure. Nanostructures having tighter size and shape compared to concatemers generated in the absence of the compaction oligonucleotides. The compact and stable characteristics of the nucleic acid nanostructures improves sequencing accuracy by increasing signal intensity and they retain their shape and size during multiple sequencing cycles.

The invention provides a biosensor based on a screen-printed electrode as well as a preparation method and application of the biosensor. The preparation method comprises the following steps: a, depositing a silicon thin film transition layer on the surface of a working electrode of a screen-printed electrode by adopting PECVD (Plasma Enhanced Chemical Vapor Deposition) equipment; b, spin-coating a tin dioxide solution on the surface of the silicon thin film transition layer to form a tin dioxide layer; c, hydrogen reduction is firstly carried out on the surface of the tin dioxide layer, then silicon nanowire growth is carried out, and a silicon nanowire layer is obtained; and d, firstly carrying out ultraviolet ozone treatment on the silicon nanowire layer, then putting the silicon nanowire layer into an alcoholic solution containing 3-aminopropyltriethoxysilane to carry out condensation reaction, cleaning, then heating to enhance the coupling strength, and finally putting the silicon nanowire layer into a phosphate buffer solution containing a nucleic acid aptamer to fix, thereby obtaining the biosensor. The silicon nanowire is prepared on the screen-printed electrode, then the nucleic acid aptamer is fixed, the electrochemical sensor for detecting the Alzheimer's disease is constructed, and the electrochemical sensor has excellent conductivity, biocompatibility and detection sensitivity.

Provided is a method for detecting a nucleic acid of interest in a sample. The method includes steps of: (i) producing a labeled nucleic acid having a first adapter at its 5' or 3' ends; (ii) applying the labeled nucleic acid of step (i) onto a substrate having an electrode disposed thereon, in which the electrode is coated with a second adapter configure to capture the first adapter thereby immobilizing the nucleic acid of interest onto the electrode; (iii) contacting the immobilized nucleic acid of interest of step (ii) with a composition comprising a CRISPR / Cas protein and a guide RNA specific to a segment of the nucleic acid of interest to initiate cis-cleavage of the immobilized nucleic acid of interest by the CRISPR / Cas protein; and (iv) measuring a signal of the electrode after the cis-cleavage. The method is characterized by not using a reporter to detect the cleaved product after the cis-cleavage.

The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and / or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.

The present disclosure provides for capturing nucleic acid from a cellular sample on a support, preparing a library molecule on the support, amplifying the library molecule on the support to produce a nucleic acid template molecule, and analyzing the immobilized nucleic acid template molecule, comprising detecting and / or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecule corresponds to the nucleic acid from the cell sample. The immobilized nucleic acid template molecule is spatially located on the support at a position corresponding to a spatial position of the nucleic acid from the cell sample.

The application provides a biosensor based on a screen-printed electrode and a preparation method and application thereof. The preparation method comprises the following steps: a, depositing a silicon thin film transition layer on the surface of a working electrode of a screen-printed electrode by using a PECVD device; b, spin-coating a tin dioxide solution on the surface of the silicon thin film transition layer to form a tin dioxide layer; c, first performing hydrogen reduction on the surface of the tin dioxide layer, and then performing silicon nanowire growth to obtain a silicon nanowire layer; d, first performing ultraviolet ozone treatment on the silicon nanowire layer, then placing the silicon nanowire layer in an alcohol solution containing 3-aminopropyl triethoxysilane to perform condensation reaction, cleaning, then performing heating to enhance the coupling strength, and finally placing the silicon nanowire layer in a phosphate buffer solution containing a nucleic acid aptamer to fix the nucleic acid aptamer, so as to obtain the biosensor. The application prepares silicon nanowires on a screen-printed electrode, then fixes a nucleic acid aptamer, and constructs an electrochemical sensor for detecting Alzheimer's disease, and the electrochemical sensor has excellent conductivity, biocompatibility and detection sensitivity.

The application discloses a kind of fluorescent probe devices with high sensitivity detection in the field of biological fluorescence detection, including microfluidic chip, closed microfluid channel for test sample flow is equipped inside microfluidic chip, microfluid channel includes reaction bin, sample bin and enzyme liquid bin, sample bin and enzyme liquid bin are symmetrically distributed on the both sides of reaction bin and are connected with the delay channel of zigzag structure between reaction bin, first filling opening and second filling opening are respectively opened in sample bin and enzyme liquid bin, reaction bin is opened with the vent hole being communicated with outside, and a plurality of aptamer fluorescent probes are fixed on the bottom wall of reaction bin.The application fixes nucleic acid aptamer fluorescent probe in microfluidic chip, and adds CRISPR-Cas12a enzyme cutting system, releases multiple signal fragments in the process of single aptamer binding antibiotic, realizes signal cascade amplification of CRISPR-Cas12a non-specific enzyme cutting activation, and has the function of detecting the existence of drug-resistant gene, to minimize the possibility of false negative detection results.