6 results about "Immobilized Nucleic Acids" patented technology

The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and / or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.

The present disclosure provides compositions and related methods, e.g., for preparing immobilized nucleic acid nanostructures using compaction oligonucleotides. In some embodiments, rolling circle amplification reaction can be conducted with compaction oligonucleotides on-support or in-solution to generate concatemer molecules having multiple copies of a polynucleotide unit arranged in tandem. Each polynucleotide unit comprises a sequence-of-interest and at least one universal adaptor sequence that binds one end of a compaction oligonucleotide. The 5′ and 3′ regions of the compaction oligonucleotide can hybridize to the concatemer to pull together distal portions of the concatemer causing compaction of the concatemer to form a nanostructure. Nanostructures having tighter size and shape compared to concatemers generated in the absence of the compaction oligonucleotides. The compact and stable characteristics of the nucleic acid nanostructures improves sequencing accuracy by increasing signal intensity and they retain their shape and size during multiple sequencing cycles.

The invention provides a biosensor based on a screen-printed electrode as well as a preparation method and application of the biosensor. The preparation method comprises the following steps: a, depositing a silicon thin film transition layer on the surface of a working electrode of a screen-printed electrode by adopting PECVD (Plasma Enhanced Chemical Vapor Deposition) equipment; b, spin-coating a tin dioxide solution on the surface of the silicon thin film transition layer to form a tin dioxide layer; c, hydrogen reduction is firstly carried out on the surface of the tin dioxide layer, then silicon nanowire growth is carried out, and a silicon nanowire layer is obtained; and d, firstly carrying out ultraviolet ozone treatment on the silicon nanowire layer, then putting the silicon nanowire layer into an alcoholic solution containing 3-aminopropyltriethoxysilane to carry out condensation reaction, cleaning, then heating to enhance the coupling strength, and finally putting the silicon nanowire layer into a phosphate buffer solution containing a nucleic acid aptamer to fix, thereby obtaining the biosensor. The silicon nanowire is prepared on the screen-printed electrode, then the nucleic acid aptamer is fixed, the electrochemical sensor for detecting the Alzheimer's disease is constructed, and the electrochemical sensor has excellent conductivity, biocompatibility and detection sensitivity.

The present disclosure provides compositions, apparatuses and methods for capturing on a support nucleic acids from cellular samples, preparing library molecules on the support, amplifying the library molecules on the support to generate nucleic acid template molecules, and analyzing the immobilized nucleic acid template molecules including detecting and / or sequencing the immobilized nucleic acid template molecules. The immobilized nucleic acid template molecules correspond to the nucleic acids from the cellular samples. The immobilized nucleic acid template molecules are spatially located on the support at positions that correspond to the spatial location of the nucleic acids from the cellular sample.

The application provides a biosensor based on a screen-printed electrode and a preparation method and application thereof. The preparation method comprises the following steps: a, depositing a silicon thin film transition layer on the surface of a working electrode of a screen-printed electrode by using a PECVD device; b, spin-coating a tin dioxide solution on the surface of the silicon thin film transition layer to form a tin dioxide layer; c, first performing hydrogen reduction on the surface of the tin dioxide layer, and then performing silicon nanowire growth to obtain a silicon nanowire layer; d, first performing ultraviolet ozone treatment on the silicon nanowire layer, then placing the silicon nanowire layer in an alcohol solution containing 3-aminopropyl triethoxysilane to perform condensation reaction, cleaning, then performing heating to enhance the coupling strength, and finally placing the silicon nanowire layer in a phosphate buffer solution containing a nucleic acid aptamer to fix the nucleic acid aptamer, so as to obtain the biosensor. The application prepares silicon nanowires on a screen-printed electrode, then fixes a nucleic acid aptamer, and constructs an electrochemical sensor for detecting Alzheimer's disease, and the electrochemical sensor has excellent conductivity, biocompatibility and detection sensitivity.

The application discloses a kind of fluorescent probe devices with high sensitivity detection in the field of biological fluorescence detection, including microfluidic chip, closed microfluid channel for test sample flow is equipped inside microfluidic chip, microfluid channel includes reaction bin, sample bin and enzyme liquid bin, sample bin and enzyme liquid bin are symmetrically distributed on the both sides of reaction bin and are connected with the delay channel of zigzag structure between reaction bin, first filling opening and second filling opening are respectively opened in sample bin and enzyme liquid bin, reaction bin is opened with the vent hole being communicated with outside, and a plurality of aptamer fluorescent probes are fixed on the bottom wall of reaction bin.The application fixes nucleic acid aptamer fluorescent probe in microfluidic chip, and adds CRISPR-Cas12a enzyme cutting system, releases multiple signal fragments in the process of single aptamer binding antibiotic, realizes signal cascade amplification of CRISPR-Cas12a non-specific enzyme cutting activation, and has the function of detecting the existence of drug-resistant gene, to minimize the possibility of false negative detection results.