56 results about "Oligonucleotide Primer" patented technology

The present disclosure generally relates to a method for rapidly detecting the presence or absence of serotypes that cause lymphogranuloma venereum (LGV) of Chlamydia trachomatis in biological or non-biological samples. The method may include performing an amplification step, a hybridization step, and a detection step. Further provided are oligonucleotide primers and probes targeting the pmpH gene of the L serotype of Chlamydia trachomatis, as well as a kit designed to detect the L serotype.

The invention relates to two compounds for RNA capping and application thereof, and belongs to the technical field of genetic engineering. The compound has a structure as shown in a formula (I), can be used as an initial capping oligonucleotide primer, is used for capping an mRNA 5'end, and has good capping efficiency and protein expression quantity; meanwhile, due to the fact that the fluorescent group marker is arranged, the fluorescent group marker can be used for detecting and tracking dynamic changes of mRNA in cells and living bodies, the delivery process, pharmacokinetics and other performance of mRNA in the cells or living bodies can be conveniently researched, and convenience is provided for research of mRNA vaccines / drugs in the cells or living bodies. # imgabs0 #

The invention provides a system and methods of multiplexed, solid-phase isothermal nucleic acid amplification. In various aspects, the invention uses a microfluidic device that includes a field of actuatable microposts in a reaction (or assay) chamber to enhance fluid flow, mixing, and hybridization / capture efficiency in a solid-phase capture assay. In various other aspects, the invention uses oligonucleotide primers immobilized in a field of actuatable microposts in a reaction chamber of a microfluidics device for capture and amplification of target-specific nucleic acids in a sample fluid. The invention provides methods of producing a micropost field (array) on a substrate for printing of a capture array (e.g., an array of primer spots). The invention also provides methods of printing an array of capture spots (e.g., primer spots) on the substrate surface of a micropost field.

A composition for identifying a hemp cultivar Pinkpepper or a progeny plant thereof includes the oligonucleotide primer set of SEQ ID NOs: 7 and 8, and / or the oligonucleotide primer set of SEQ ID NOs: 9 and 10. A method for identifying hemp cultivar Pinkpepper or a progeny plant thereof includes extracting genomic DNA from a hemp plant, amplifying a target sequence by having the extracted genomic DNA as template and carrying out an amplification reaction using the oligonucleotide primer set, and detecting a product produced by the application of the target sequence.

Flow cells and methods are described that enable control over DNA fragment length bias during amplification. One example flow cell includes a substrate and a polymeric hydrogel positioned over at least a portion of the substrate. A plurality of oligonucleotide primers may be attached to the polymeric hydrogel, wherein the plurality of oligonucleotide primers defines a density gradient across the substrate of the flow cell.

The invention provides methods for simultaneously enriching multiple target regions of interest in one reaction volume, from cell-free DNA isolated from a blood or plasma sample, followed by high-thought sequencing and sequence read analysis. The invention also provides library of target-specific oligonucleotide primers or probes for the multiplexed target enrichment.

The present disclosure provides for methods of generating a single stranded primer from an oligonucleotide probe. For instance, the present disclosure is directed to a method of preparing oligonucleotide primers from oligonucleotide probes, such as oligonucleotide probes which have been amplified, and which include a target specific primer sequence, a forward primer binding site, and a reverse primer binding site. It is believed that the presently disclosed method permits the synthesis of thousands to hundreds of thousands and even a million primers in a cost-effective manner, with good yield, in high quantities and at high purity levels.

The present application relates to a kind of oligonucleotide primer probe compositions for detecting Pseudomonas syringae, the pathogen of vegetable leaf spot disease based on ERA fluorescence method or ERA test strip method, and the detection method using the composition.The present application can simply, quickly, specifically, sensitively and visually broad-spectrum identify a variety of pathogenic variants of Pseudomonas syringae, the pathogen of vegetable leaf spot disease, the method is easy to operate, reaction time is short, without complex temperature control equipment, can be widely applied to the on-site rapid screening and disease diagnosis of Pseudomonas syringae in vegetable sample.

Disclosed herein are novel methods and compositions for rapidly extracting and amplifying nucleic acids from a sample where the sample is combined with an extraction reagent comprising a reducing agent to form a mixture and incubating said mixture at ambient temperature for a period of time not exceeding 30 minutes to generate a nucleic acid extract. In certain embodiments of the method, the nucleic acid extract is subjected to a nucleic acid amplification reaction. In certain aspects, oligonucleotide primers specific for nucleic acids of Chlamydia species and / or Neisseria species are added prior to initiating the amplification reaction.

The embodiment of the invention discloses a TaqMan real-time fluorescent quantitative PCR probe primer combination for identifying tilapia mossambica, a kit and application. Comprising a probe, an upstream primer and a downstream primer, the nucleotide sequence of the probe is 5 '-FAM-CGCTATACAGGG-MGB-3', the nucleotide sequence of the upstream primer is 5 '-GACTTTCCCCCATATTC-3', and the nucleotide sequence of the downstream primer is 5 '-GGGGTAGACAGTTCACCCTGTA-3'. Based on difference of mitochondrial control areas of different fishes, the TaqMan probe real-time fluorescent PCR detection method established by designing specific oligonucleotide primers and probes can effectively distinguish tilapia homosambica from other fishes, has excellent specificity and sensitivity in the aspect of identifying tilapia homosambica species, and can be used for detecting the species of tilapia homosambica. The detection accuracy is greatly improved, meanwhile, the detection time is shortened, the effects of stability, reliability, short period and low cost are achieved, and the actual application requirements are met.

Provided herein are methods and compositions for the synthesis of 5 '-capped RNA wherein the starting capped oligonucleotide primer has the general form m7Gppp [N2' Ome] n [N] m wherein m7G is an N7-methylated guanosine or any guanosine analog, N is any natural, modified or non-natural nucleoside, "n" may be any integer from 0 to 4 and "m" may be an integer from 1 to 9.

The present invention relates to the detection of modifications in polynucleotides by binding an affinity binding element to a nucleotide sequence. The affinity binding element may include a compound that binds to a modified nucleotide base and a nucleotide sequence complementary to a target nucleotide sequence, an oligonucleotide primer complementary to the target nucleotide sequence, or a nucleotide sequence complementary to an oligonucleotide linker. The present invention provides methods for detecting one or more modifications in a nucleotide sequence. The invention also provides affinity binding elements, and optionally, oligonucleotide primers and / or oligonucleotide probes, and methods of using the affinity binding elements in assays to detect modified bases. The methods of the invention enable the detection of modified nucleotides associated with cancer, psychiatric disorders or metabolic diseases.

The present invention is directed to a method for detecting the presence or absence of monkeypox virus (MPXV) in a biological sample, the method comprising: a) contacting the sample or nucleic acid extracted from said sample with reagents and an oligonucleotide combination capable of amplifying a target region of a monkeypox viral genome, wherein said oligonucleotide combination is selected from the group consisting of: i) a B7R oligonucleotide primer set specific for amplifying a target sequence in the B7R gene of the MPXV genome generating an MPXV amplification product comprising the sequence of SEQ ID NO:65; and ii) a G2R oligonucleotide primer set specific for amplifying a target sequence in the G2R gene of the MPXV genome generating an MPXV amplification product comprising the sequence of SEQ ID NO:63; b) performing an in vitro nucleic acid amplification reaction, wherein any MPXV nucleic acid present in the sample is used as a template to generate the MPXV amplification product corresponding to the oligonucleotide combination of i) or ii); and c) detecting the presence or absence of the MPXV amplification product generated in step b), wherein the presence of the MPXV amplification product is indicative of the presence of MPXV in the biological sample. The present invention also provides oligonucleotides which can be used in the method of the invention as primers and probes.

The present disclosure relates generally to devices and methods for rapid and accurate diagnosis of single nucleotide polymorphisms. According to one or more embodiments, a system for identifying a single nucleotide polymorphism (SNP) comprises an oligonucleotide primer having a SNP recognition sequence, a fluorophore conjugated to the oligonucleotide primer at a conjugation site to form a fluorophore-conjugated oligonucleotide primer, a polymerase for extending the oligonucleotide primer to form a double-stranded deoxyribonucleic acid (DNA) amplicon upon binding to an oligonucleotide strand having a complementary sequence to the oligonucleotide primer, and wherein the fluorophore-conjugated oligonucleotide primer is the only primer in the system such that the fluorophore emits a fluorescent signal only when the fluorophore-conjugated oligonucleotide primer binds to a sequence specifically complementary to the SNP recognition sequence.

The invention relates to a set of oligonucleotide primers, and kits and in vitro diagnostic devices comprising the set of oligonucleotide primers. The set of oligonucleotide primers, kits and in vitro diagnostic devices can be used in detecting Neisseria gonorrhoeae nucleic acid and diagnosing Neisseria gonorrhoeae infection with high sensitivity and specificity. The invention also relates to methods of loop-mediated isothermal amplification, methods of detecting Neisseria gonorrhoeae nucleic acid, and methods of diagnosing Neisseria gonorrhoeae infection using the set of oligonucleotide primers or the kits.

To provide oligonucleotide primers and oligonucleotide probes that specifically amplify and detect nucleic acids derived from Mycobacterium kansasii group present in a sample, and a detection method using the same.SOLUTION: The present invention provides a primer set as oligonucleotides for detecting a specific nucleotide sequence or its complementary sequence of DNA or RNA derived from Mycobacterium kansasii group, the primer set comprising at least a combination of a first primer being SEQ ID NO:8 or SEQ ID NO:11 and a second primer being SEQ ID NO:6 or SEQ ID NO:9, and a detection method using the same.SELECTED DRAWING: None

Disclosed are oligonucleotide primers that hybridize specifically with any base sequence designed from the base sequences of the N gene, RNA-dependent RNA polymerase gene, M gene, and S gene of SARS-COV-2, a nucleic acid amplification method using said primers, a test method for SARS-COV-2 infection by detection of nucleic acid amplification, and a COVID-19 test kit.

The present disclosure relates generally to devices and methods for rapidly and accurately diagnosing single-nucleotide polymorphisms. According to one or more embodiments, a system for identifying a single-nucleotide polymorphism (SNP) includes an oligonucleotide primer with a SNP recognition sequence, a fluorophore conjugated to the oligonucleotide primer at a conjugation site to form a fluorophore-conjugated oligonucleotide primer, a polymerase enzyme to extend the oligonucleotide primer upon binding to an oligonucleotide strand with a complimentary sequence to the oligonucleotide primer, forming a double stranded deoxyribonucleic acid (DNA) amplicon, and wherein the fluorophore-conjugated oligonucleotide primer is a sole primer in the system, such that the fluorophore only emits a fluorescent signal when the fluorophore-conjugated oligonucleotide primer binds to a sequence specifically complementary to the SNP recognition sequence.

The subject application pertains to compositions and methods for enhancing reverse transcriptase (RT) activity and / or reducing the inhibition of RT by inhibitors, such as formalin, tannic acid and / or heparin. In some embodiments, RT inhibition is reduced by the addition of potassium glutamate, histidine hydrochloride monohydrate, poloxamer 188, or any combination thereof to a reaction mixture comprising a polymerase. In other embodiments, RT is enhanced through the addition of a polyvinyl sulfonic acid sodium salt (PVSA) to a reaction mixture. The subject application also provides oligonucleotide primers for use in the reverse transcription of target sequences and its enhancement. These primers have high GC content or low GC content. Methods of using a RT inhibition reducer or a RT enhancer in a composition with an RNA template and RT improves RT yield, RT sensitivity, or RT tolerance to various chemicals are also provided.

The present invention relates to methods for the detection of nucleic acids of defined sequence and kits and devices for use in said methods. The methods employ restriction enzymes. polymerase and oligonucleotide primers to produce an amplification product in the presence of a target nucleic acid. which is contacted with oligonucleotide probes to produce a detector product.

The present disclosure relates to materials and methods for capturing a target nucleic acid sequence, comprising annealing a first target-specific oligonucleotide primer to a target sequence; and extending the 3'end of the first target specific oligonucleotide primer so as to linearly amplify the target nucleic acid sequence. Then, annealing the second target specific oligonucleotide primer into an amplified target sequence; and extending the 3'end of the second target specific oligonucleotide primer so as to linearly amplify the complement of the target nucleic acid sequence. The resulting copy of the target nucleic acid sequence can be detected or sequenced. A plurality of target nucleic acid sequences from one or more samples may also be captured. A unique identifier sequence may be introduced to track the source of the captured target nucleic acid sequence. The invention also provides kits for performing the methods disclosed herein.

The present invention provides methods and compositions for determining the presence and / or amount of pathogenic Leptospira in a test sample. In particular, substantially purified oligonucleotide primers and probes are described that can be used for qualitatively and quantitatively detecting pathogenic Leptospira nucleic acid in a test sample by amplification methods. The present invention also provides primers and probes for generating and detecting control nucleic acid sequences that provide a convenient method for assessing internal quality control of the Leptospira assay.

A method of amplifying a target nucleic acid in a DNA sample comprising (a) contacting the DNA sample containing the target nucleic acid with a DNA polymerase, at least two oligonucleotide primers designed to flank the target nucleic acid, a mixture of dATP, dGTP, dCTP, and dTTP, and a conjugate comprising a DNA polymerase inhibitor covalently attached to a negative temperature sensitive polymer, (b) heating the output of step (a) to a temperature at which the conjugate precipitates and thus the DNA polymerase is no longer inhibited and (c) amplifying the target nucleic acid, e.g. by performing PCR steps of denaturing the target nucleic acid, annealing the primers to the target nucleic acid, and extending the primers, wherein step (c) is repeated at least two times. Also provided is a conjugate comprising a DNA polymerase inhibitor covalently attached to a negative temperature sensitive polymer and kits and aqueous compositions comprising the same.

This invention claims processes that append a single nucleotide having a 3′-ONH2 moiety to the 3′-ends of an oligonucleotide primer using 3′-deoxynucleoside triphosphates that have, instead of a 3′-OH moiety, a 3′-ONH2 moiety, where the nucleotides contain both standard and non-standard nucleobases, and where as a key claim limitation, substantially no hydroxylamine is present in the solutions used in the claimed processes.

Provided herein are methods and compositions for synthesizing 5'Capped RNAs wherein the initiating capped oligonucleotide primers have the general form m7Gppp[N2'Ome]n[N]m wherein m7G is N7-methylated guanosine or any guanosine analog, N is any natural, modified or unnatural nucleoside, "n" can be any integer from 0 to 4 and "m" can be an integer from 1 to 9.

Disclosed are oligonucleotide primers that hybridize specifically with any base sequence designed from the base sequences of the N gene, RNA-dependent RNA polymerase gene, M gene, and S gene of SARS-CoV-2, a nucleic acid amplification method using said primers, a test method for SARS-CoV-2 infection by detection of nucleic acid amplification, and a COVID-19 test kit.

The invention relates to a set of oligonucleotide primers, and kits and in vitro diagnostic devices comprising the set of oligonucleotide primers. The set of oligonucleotide primers, kits and in vitro diagnostic devices can be used in detecting Chlamydia trachomatis nucleic acid and diagnosing Chlamydia trachomatis infection with high sensitivity and specificity. The invention also relates to methods of loop-mediated isothermal amplification, methods of detecting Chlamydia trachomatis nucleic acid, and methods of diagnosing Chlamydia trachomatis infection using the set of oligonucleotide primers or the kits.

Presented arc two new methods, single primer extension reaction (SSPER) and reduce recycle PCR (rrPCR). These methods have a step that easily removes the oligonucleotide primer(s) after the first reaction, thus, allowing for the addition of a second reaction in chronological sequence to generate and isolate the appropriate DNA product with the site-directed mutation(s). A high efficiency of the methods is demonstrated by generating all ten site-directed mutations and six paired combinations of these mutations on four plasmid DNA templates ranging from 10 to 12 kb and 57 to 59% GC-content at a rate of 50-100%. The methods are i) highly accurate allowing for screening of plasmids by DNA sequencing, ii) streamlined to generate the mutations within a single day, iii) cost-effective in requiring only two primers and two enzymes (Dpnl and a proofreading DNA polymerase), iv) straightforward in primer design, and v) applicable for both large and small plasmids.