6 results about "Oligonucleotide Primer" patented technology

The present application relates to a kind of oligonucleotide primer probe compositions for detecting Pseudomonas syringae, the pathogen of vegetable leaf spot disease based on ERA fluorescence method or ERA test strip method, and the detection method using the composition.The present application can simply, quickly, specifically, sensitively and visually broad-spectrum identify a variety of pathogenic variants of Pseudomonas syringae, the pathogen of vegetable leaf spot disease, the method is easy to operate, reaction time is short, without complex temperature control equipment, can be widely applied to the on-site rapid screening and disease diagnosis of Pseudomonas syringae in vegetable sample.

A method of amplifying a target nucleic acid in a DNA sample comprising (a) contacting the DNA sample containing the target nucleic acid with a DNA polymerase, at least two oligonucleotide primers designed to flank the target nucleic acid, a mixture of dATP, dGTP, dCTP, and dTTP, and a conjugate comprising a DNA polymerase inhibitor covalently attached to a negative temperature sensitive polymer, (b) heating the output of step (a) to a temperature at which the conjugate precipitates and thus the DNA polymerase is no longer inhibited and (c) amplifying the target nucleic acid, e.g. by performing PCR steps of denaturing the target nucleic acid, annealing the primers to the target nucleic acid, and extending the primers, wherein step (c) is repeated at least two times. Also provided is a conjugate comprising a DNA polymerase inhibitor covalently attached to a negative temperature sensitive polymer and kits and aqueous compositions comprising the same.

The present disclosure provides methods and compositions of modified oligonucleotide primer and probe combinations, structurally modified with locked nucleic acids, quenchers, and dyes, effective to detect tumor-derived Human Papilloma Virus (HPV) and tumor-derived Epstein-Barr virus (EBV) and, especially, to distinguish viral DNA derived from tumors from viral DNA derived from infectious viral particles.

The present application relates to a single fluorescence method and a double fluorescence method for rapid detection of Cronobacter dublinensis and Cronobacter univans by ERA technology. The present application also relates to an oligonucleotide primer probe composition for the method. The present application also relates to a detection kit comprising the composition. The ERA fluorescence method detection using the composition of the present application can simply, rapidly, specifically and sensitively detect Cronobacter dublinensis and Cronobacter univans.

The present application relates to the single fluorescence method and double fluorescence method for rapid detection of Cronobacter sakazakii and Cronobacter zurichensis by using ERA technology. The present application also relates to the oligonucleotide primer probe composition for the method. The present application also relates to the detection kit comprising the composition. The ERA fluorescence method detection using the composition of the present application can simply, rapidly, specifically and sensitively detect Cronobacter sakazakii and Cronobacter zurichensis.