48 results about "Nucleotide composition" patented technology

The invention belongs to the field of gene engineering, and discloses a pythium ultimum PuRWP1 gene and application thereof as a molecular target in prevention and treatment of diseases caused by pythium ultimum. The invention provides a gene PuRWP1 which is from pythium ultimum and is used for controlling a sexual development stage and oospore formation, the gene is composed of 3026 nucleotides, a protein coded by the PuRWP1 gene is composed of 217 amino acids, and knockout of the PuRWP1 gene can lead to remarkable reduction of sporulation ability of a mutant and reduction of spore activity, so that the mutant can be used for preparing a new mutant. The gene can be used as a molecular target to be applied to disease control of oomycetes. The function of the pythium ultimum PuRWP1 gene is verified, and a new direction is provided for further utilizing the gene to regulate and control the physiological functions of oomycetes such as pythium ultimum and preventing and treating oomycetes diseases.

The application discloses application of a rice disease resistance related gene Os04g55720 in bacterial blight breeding and relates to the technical field of modern biotechnology and genetic engineering technology.The rice disease resistance related gene Os04g55720 has a nucleotide sequence as shown in SEQ ID NO.1, is composed of 2821 nucleotides, has a coding region sequence as shown in SEQ ID NO.2, and has a sequence of 2148 nucleotides; the gene encodes a protein with 613 amino acids, and has an amino acid sequence as shown in SEQ ID NO.3.The application proves that overexpression of Os04g55720 enhances the resistance of rice to bacterial blight by means of molecular biology and biochemical technology, and shows that the rice protein OsPGDH-3 can effectively control the incidence of rice bacterial blight at a relatively low level.The application provides a new idea, strategy and gene resource for green prevention and control of rice bacterial blight.

The application discloses a set of general primers for amplifying the whole genome of the mitochondrion of Trionychidae, and belongs to the technical field of molecular biology; the specific steps comprise the following steps: extracting the total DNA of the genome of Trionychidae, designing the general amplification primers of the whole sequence of the mitochondrial genome, and performing PCR amplification and sequencing on the mitochondrial genome DNA. The amplification primers for the whole genome sequence of the mitochondrion of Trionychidae are composed of 11 pairs of single-stranded oligonucleotides. The general amplification primers of the application can be used to quickly and efficiently amplify the whole sequence of the mitochondrial genome of various Trionychidae, and provide a favorable technical means for the identification of unknown germplasm of Trionychidae, the protection of species resources, and the research on the system evolution.

The application discloses a specific nucleic acid aptamer for a heterogeneous core ribonucleoprotein hnRNPA2B1 and application, and belongs to the technical field of nucleic acid aptamer. The hnRNPA2B1 aptamer is a small single-stranded DNA screened from a designed oligonucleotide library through an in-vitro exponential enrichment ligand system evolution (SELEX), is composed of 80 nucleotides, and has high affinity to the target. The hnRNPA2B1 aptamer can be applied in a detection method or a detection product of the hnRNPA2B1 protein, and a targeted regulation drug or a biological preparation, and can be specifically made into a molecular probe, used as a detection reagent, used as a targeted preparation and the like for diagnosis and treatment of diseases related to the hnRNPA2B1 protein. The hnRNPA2B1 aptamer has strong affinity and specificity, has the advantages of low production cost, stability, convenient synthesis and easy modification compared with an antibody, and is a potential substitute for the antibody in disease diagnosis and treatment.

The AgDoa gene and application thereof in reducing the pesticide resistance of cotton aphids belong to the technical field of biological genetic engineering, the DNA sequence of the AgDoa gene is shown as SEQ ID No:1, and the AgDoa gene is composed of 1782 nucleotides; the amino acid sequence of the protein coded by the AgDoa gene is shown as SEQ ID No:2, and the protein is composed of 593 amino acids; the AgDoa gene can be used as a target to develop a new pesticide; the tolerance of the cotton aphids to the pesticide is reduced by knocking down the AgDoa gene, and the AgDoa gene can be applied in the resistance management and development of new pesticides.

Methods, kits, and oligonucleotides used in the detection of coronavirus, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are disclosed. In some aspects, the oligonucleotides are primers or probes used in the described methods or kits. The nucleotide sequence of the oligonucleotide consists of 300 or less, 150 or less, or 40 or less continuous nucleotides from a nucleotide sequence selected from the group consisting of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO: 5, or is a variant thereof. In some embodiments, the oligonucleotide is modified with an internal spacer or a detectable label. For example, the 5′ terminus is labeled with a fluorophore and the 3′ terminus is complexed to a quencher of fluorescence of said fluorophore. In some embodiments, the nucleotide sequence of the oligonucleotide further comprises a universal tail sequence.

In the present specification, an antisense oligonucleotide or a pharmaceutically acceptable salt thereof, or a hydrate thereof, consisting of 15 to 22 nucleotides complementary to a nucleic acid comprising at least 15 consecutive bases in a target region selected from the group consisting of positions 1 to 102, 159 to 842, 879 to 1822, and 1874 to 4182 from the 5′ end of a base sequence of SEQ ID NO: 1 is provided.

RNA molecules comprising a guide sequence portion having 17-20 nucleotides in the sequence of 17-20 contiguous nucleotides set forth in any one of SEQ ID Nos: 1-1313 and compositions, methods, and uses thereof.

Methods, kits, and oligonucleotides used in the detection of the coronavirus strain, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are disclosed. In some aspects, the oligonucleotides are primers or probes used in the described methods or kits. The oligonucleotide consists of 40 or less nucleotides and has a nucleotide sequence that consists essentially of, or is a variant of, the nucleotide sequence of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7. In some embodiments, the oligonucleotide is modified with an internal spacer or a detectable label. For example, the 5′ terminus is labeled with a fluorophore and the 3′ terminus is complexed to a quencher of fluorescence of said fluorophore. In some embodiments, the nucleotide sequence of the oligonucleotide further comprises a universal tail sequence.

A method of quantifying RNA capping efficiency, the method comprising: (a) providing a sample of capped RNA; (b) contacting the capped RNA with a nuclease, wherein the nuclease is a protein, thereby hydrolysing the RNA to produce hydrolysis products comprising a capped product comprising dinucleotides, and an uncapped product comprising nucleotides, step (b) being carried out in the absence of a nucleic acid having a base sequence complementary to the sequence of the capped RNA; (c) separating the hydrolysis products by chromatography; and (d) determining the concentrations of the hydrolysis products by triple quadrupole mass spectrometry, thereby quantifying RNA capping efficiency; is provided.

The present technology relates to compositions and methods for modulating expression of genes, which include a target oligonucleotide sequence, such as repeats of a particular oligonucleotide sequence containing 3 to 10 nucleotides. In particular aspects, the present technology relates to agents having a formula A-L-B, wherein -L- is a linker; A- is a Brd4 binding moiety; and —B is a nucleic acid binding moiety, such as a polyamide or complementary oligonucleotide, that specifically binds to the target oligonucleotide sequence.

An antitumor agent containing, as an active ingredient, RNA having a base sequence selected from the group consisting of (1) and (2) below, and having at least one nucleotide unit represented by Formula (1) below in a 3′ end region within 20 bases of the 3′ end thereof and / or in a 5′ end region within 25 bases of the 5′ end thereof.(1) A base sequence represented by SEQ ID NO: 2 or a base sequence having 90% or more identity with such sequence(2) A base sequence consisting of between 20 and 200 consecutive nucleotides of the base sequence described in (1) above(In Formula (1), R1 represents a hydroxyl group, a hydroxyl group with a hydrogen atom substituted with an alkyl or alkenyl group, or a halogen atom; R2 represents NHR3 with a linking group, the linking group being a divalent hydrocarbon group with one or more carbon atoms; R3 represents a hydrogen atom, an alkyl group, or an alkenyl group; n is 0 or 1; X1 represents an oxygen atom or sulfur atom; X2 represents OH (or O−) or SH (or S−); and B represents a purine base or a pyrimidine base.)

A polynucleotide comprising a modified poly (A) having a number of nucleotides of at least 30, the modified poly (A) comprising: i) at least two R elements, the R elements defined as polynucleotides consisting of A nucleotides; and ii) at least one S element wherein S element: a) is one nucleotide that is not the A nucleotide, or b) consists of at least two nucleotides wherein neither of the nucleotides at the two ends is the A nucleotide; wherein the total number of S elements is one less than the total number of R elements, the S elements are connected to the R elements, and both sides of each S element are the R elements. The invention also relates to a vector containing the polynucleotide, a host cell, a method for preparing RNA by using the polynucleotide, the RNA prepared by using the method, a pharmaceutical composition containing the polynucleotide, the RNA, the vector and / or the host cell, an LNP and application of the LNP.

The present invention provides a tau exon 10 skipping-promoting antisense oligonucleotide containing at least one 2′-O, 4′-C-ethylene-bridged nucleic acid, and a nucleotide sequence complementary to a sequence consisting of at least 10 continuous nucleotides in a region consisting of the nucleotide sequence shown in SEQ ID NO: 44 in exon 10 of a tau mRNA precursor. In addition, the present invention provides a tau exon 10 skipping-suppressing antisense oligonucleotide containing at least one 2′-O, 4′-C-ethylene-bridged nucleic acid, and a nucleotide sequence complementary to a sequence consisting of at least 10 continuous nucleotides in a region consisting of the nucleotide sequence shown in SEQ ID NO: 45 in intron 10 of a tau mRNA precursor.

The invention discloses a primer pair, a kit and a detection method for detecting ganoderma lucidum decay monascus xylophilus and application. The invention discloses a primer pair for detecting ganoderma lucidum spoilage monascus xylogenus. The primer pair is composed of a nucleotide as shown in SEQ ID NO.1 and a nucleotide as shown in SEQ ID NO.2. The invention further discloses a method for detecting ganoderma lucidum spoilage monascus xylogenus. The nucleotide is composed of a nucleotide shown in SEQ ID NO.3 or a nucleotide shown in SEQ ID NO.3 and a nucleotide shown in SEQ ID NO.4. The invention also discloses a kit comprising the primer pair, a method for detecting the ganoderma lucidum spoilage monascus xylogenus in a sample, a method for quantitatively detecting the content of the ganoderma lucidum spoilage monascus xylogenus in the sample and application of the primer pair. The primer and the probe with high specificity are designed, so that pathogenic bacteria causing the yellow foot disease can be accurately and quickly detected from a ganoderma lucidum sample, and the cross reaction with the DNA of the ganoderma lucidum is effectively avoided. The method is high in sensitivity, can realize early diagnosis and quantitative analysis, is matched with a kit for use, is simple and convenient to operate, provides a reliable technical means for timely early warning and accurate prevention and control of the ganoderma lucidum yellow foot disease, and is beneficial to remarkably reducing economic loss caused by the disease.

Molecular anti-counterfeiting taggants are made from a plurality of synthetic polynucleotides that collectively encode a bit sequence using the sequences and hybridization states of the polynucleotides. The polynucleotide taggant is placed on an item as a molecular identifier of authenticity. The bit sequence encoded by the polynucleotide taggant is read out using a substrate which has bound polynucleotides complexes that hybridize with the synthetic polynucleotides in the polynucleotide taggant. A detectable signal is present where hybridization occurs. To prevent a bad actor from reverse engineering and creating a copy of the polynucleotide taggant using the results of hybridization to the substrate, multiple versions of the substrate are created. Each version hybridizes to different subsets of the synthetic polynucleotides in the polynucleotide taggant. A detectable pattern on the substrate that is present when exposed to the polynucleotide taggant is used for validating authenticity of the item.

The invention relates to the technical field of biological information, in particular to a multi-task gene regulation network model training method based on knowledge graph embedding and feature fusion. Implicit important information in a gene sequence is captured from three aspects of context perception representation, physicochemical properties and pseudo dinucleotide composition of the sequence, and the richness of feature embedding is improved; a bidirectional long-short-term memory network is adopted to carry out splicing learning on the extracted features so as to reduce the coding difficulty of a high-throughput gene sequence; two learning task modules with different emphasis are designed and focus on node features and edge features respectively, and more comprehensive feature utilization is realized through a multi-task learning mode; the loss of the two tasks is dynamically balanced by adopting a gradient normalization algorithm, so that the overall learning efficiency and stability of the model are improved. The invention aims to solve the problem of how to improve the regulation relation prediction accuracy of the gene regulation network.

Compositions and methods for increasing the solubility and stability of organic nucleotide materials via microencapsulation techniques. A method for making a shelf-stable organic nucleotide compound in a more water-soluble form, the higher-solubility form being provided in liquid and / or powder. The method utilizes a colloidal dispersion solution comprising water, polysaccharides, organic nucleotide and in some cases surfactants. A method for making shelf-stable composition comprising at least one organic nucleotide composition may include combining water, surfactant, polysaccharide, and ATP to form an intermixed colloidal dispersion. The method may include heating the admixed dispersion and running the dispersion under medium shear, wherein the colloidal dispersion provides improved shelf stability to the target nucleotide in water-soluble form.

The invention discloses a nucleic acid composition for detecting an isoniazide drug gene based on a nucleic acid mass spectrometry technology, a kit and application thereof, and relates to the technical field of gene detection. The nucleic acid composition disclosed by the invention comprises a multiple amplification nucleotide composition and an extension nucleotide composition, wherein the multiple amplification nucleotide composition is used for carrying out multiple PCR (Polymerase Chain Reaction) amplification on sites of rs1801280, rs1799930, rs1799931, rs1799929, rs1041983 and rs1801279 of an isoniazide drug gene NAT2, and the extension nucleotide composition is used for carrying out a single base extension reaction. By using the nucleotide composition and the kit provided by the invention, the genotype of the 6-site of the isoniazide drug gene NAT2 can be detected by adopting a nucleic acid mass spectrometry technology, and the nucleotide composition and the kit have the characteristics of high sensitivity, good specificity, short detection period and low detection cost. The invention provides a more reliable and low-cost detection kit for detecting related genes of isoniazide medication, and a detection result provides a basis for accurate medication of isoniazide.

The present invention relates to: a Cas protein function-regulating switch oligonucleotide, which is an oligonucleotide that hybridizes complementarily to a spacer of a guide RNA and consists of 5 to 20 nucleotides starting from the 5' end; and a target nucleic acid amplification composition comprising same. In addition, the present invention provides the Cas protein function-regulating switch oligonucleotide, which is an oligonucleotide that hybridizes complementarily to a spacer of a guide RNA and consists of 5 to 20 nucleotides starting from the 5' end, thereby regulating the rate at which a Cas protein binds to a target nucleic acid so as to cause an RPA-CRISPR reaction to occur within a single tube without physical separation or additional steps, and thus can be used in a point-of-care diagnostic device.

RNA molecules comprising a guide sequence portion having 17-20 nucleotides in the sequence of 17-20 contiguous nucleotides set forth in any one of SEQ ID NOs: 1-1313 and compositions, methods, and uses thereof.

The application discloses a nucleic acid aptamer PCM-A09 which specifically recognizes procymidone, wherein the nucleotide sequence of the nucleic acid aptamer is shown as SEQ ID NO:1. The nucleic acid aptamer can specifically bind with procymidone. The nucleic acid aptamer PCM-A09 is single-stranded DNA (ssDNA) which is composed of 88 nucleotides. The secondary structure of the nucleic acid aptamer has outstanding stems and loops. The Gibbs free energy DG of the nucleic acid aptamer PCM-A09 is -16.14. The specificity and affinity of the nucleic acid aptamer are evaluated based on colloidal gold spectrophotometry. The experimental results show that the nucleic acid aptamer PCM-A09 can specifically bind with procymidone. The nucleic acid aptamer has the characteristics of high specificity and high affinity. The nucleic acid aptamer can be prepared into a PCM reagent, a detection kit or a sensor and applied to the detection of PCM in various agricultural products.

The invention provides an aptamer capable of specifically recognizing deoxynivalenol and application of the aptamer. The nucleotide sequence of the aptamer is as shown in SEQ ID NO: 1. According to the invention, a key site recognition mechanism of a target molecule is researched by virtue of structural biology, truncation optimization is carried out, the DON aptamer with high affinity, high specificity and high sensitivity is obtained through screening, and the DON aptamer is only composed of 31 nucleotides and has relatively strong anti-interference performance and stability, the cost is remarkably reduced, and the development of an efficient DON detection method is facilitated.

The invention discloses a molecular structure display and interaction system based on a mechanical model and digital interaction, and the system comprises a mechanical model assembly which comprises a rotatable basic group module, a ribose module and a phosphate group module, and is used for the independent combination of a user to form nucleotides; the acquisition module is arranged inside or around the base module, the ribose module and the phosphate group module and is used for detecting the spatial states of the base module, the ribose module and the phosphate group module and acquiring corresponding nucleotide composition information; the identification module is associated with the acquisition module and is configured to receive the nucleotide composition information and identify the nucleotide type formed by the user combination; and the interaction module is associated with the identification module and is used for displaying knowledge content related to the identified nucleotide type and receiving an operation instruction of a user. According to the invention, the problems of lack of interaction content and single experience form of the existing display interaction mode of related abstract microscopic concepts and knowledge contents such as molecular structures and the like can be solved.

The invention discloses siRNA for inhibiting MGAT2 gene expression and application thereof.The siRNA comprises a positive-sense strand and an antisense strand, the antisense strand has a nucleotide sequence composed of at least 15 continuous nucleotides, the difference between the nucleotide sequence shown in any one of SEQ ID NO.20-38 and the nucleotide sequence shown in any one of SEQ ID NO.20-38 and the nucleotide sequence shown in any one of SEQ ID NO.20-38 is not more than 3 nucleotides, and the nucleotide sequence shown in any one of SEQ ID NO.20-38 is a nucleotide sequence composed of at least 15 continuous nucleotides. The positive-sense strand and the antisense strand are at least partially complementary to form a double-strand region. The invention provides siRNA for inhibiting MGAT2 gene expression, the siRNA is modified to ensure the stability and inhibition activity of the siRNA, and the siRNA provided by the invention has great application prospects in preparation of drugs for preventing and / or treating diseases related to MGAT2 expression. A new treatment strategy is provided for treatment of glioblastoma, breast cancer, liver cancer and other cancers, metabolic diseases, neurodegenerative diseases and autoimmune diseases.

The application discloses an immunomodulatory peptide Ha-CATH and application thereof, and belongs to the field of biomedicine. The immunomodulatory peptide Ha-CATH is a cyclic polypeptide with a pair of intramolecular disulfide bonds formed by the eighth cysteine residue and the thirteenth cysteine residue of a skin repair peptide gene of Chinese amphibian Pelophylax nigromaculatus, has a molecular weight of 2361.8 Dalton, an isoelectric point of 6.04, and an amino acid sequence shown in SEQ ID NO:1. A gene (GenBank accession: OQ992774) encoding the precursor of the immunomodulatory peptide Ha-CATH is composed of 714 nucleotides, has a nucleotide sequence shown in SEQ ID NO:2, and the nucleotide sequence of 445-504 is the encoding gene of the immunomodulatory peptide Ha-CATH. ‑ The application also discloses an application of the immunomodulatory peptide Ha-CATH in preparation of a skin wound repair promoting therapeutic drug. ‑ The application also discloses an application of the immunomodulatory peptide Ha-CATH in preparation of a skin wound repair promoting therapeutic drug.

The application belongs to the field of genetic engineering, and discloses a final pythium PuRWP1 gene and application of the gene as a molecular target in disease prevention caused by the final pythium. The application provides a gene PuRWP1 from the final pythium for controlling a sexual development stage and oospore formation. The gene is composed of 1184 nucleotides. A protein coded by the PuRWP1 gene is composed of 627 amino acids. Knockout of the PuRWP1 gene can cause a significant decrease in sporulation capacity and a decrease in spore activity of a mutant. The gene can be used as a molecular target in disease prevention of oomycetes. The application verifies the function of the final pythium PuRWP1 gene, and provides a new direction for further regulating physiological functions of the final pythium and other oomycetes and preventing oomycete diseases by using the gene.

The application belongs to the technical field of genetic engineering, and specifically discloses a gene PoRWP2 for improving the production of Pythium oligandrum oospores and an application thereof. The application analyzes and transforms the Pythium oligandrum PoRWP2 gene, which is composed of 837 nucleotides, and the protein coded by the gene contains 278 amino acids, and can regulate the sexual development and oospore formation process of Pythium oligandrum. The application verifies the function of the PoRWP2 gene, provides a new direction for regulating the physiological function of Pythium oligandrum by using the gene and improving the sporulation capacity of the biocontrol bacteria, and provides a theoretical basis and technical support for developing biocontrol agents based on Pythium oligandrum and carrying out biological control of oomycete diseases.

This application relates to a modified dsRNA molecule and its use. Specifically, this application provides a dsRNA molecule that inhibits the expression of the Lp(a) gene, comprising a sense strand and an antisense strand that complementarily form a double-stranded region, wherein the sense strand and / or the antisense strand contain 15-25 nucleotides or consist of 15-25 nucleotides, and is useful for the treatment and / or prevention of diseases mediated by the Lp(a) gene.

The invention is directed to systems and methods of recording digital information using nucleic acids. A DNA template strand has alpha and beta segments, each with a set of three nucleotides differing from each other. When a DNA polymerase contacts the template in the presence of a first solution of three nucleotides corresponding to an alpha segment, a new strand is extended according to available base pairing. The extension halts when the polymerase encounters a beta strand, as the first solution lacks a base-pairing nucleotide triphosphate. Similarly, when the polymerase is provided with a second solution of three nucleotides corresponding to the beta segment, the new strand continues extension according to available base pairing. As first and second solutions are alternated, chemically-altered nucleotide triphosphates are introduced, providing mutations in certain segments of the new strand. When sequenced, mutation segments are assigned a '1' and non-mutation segments are assigned a 'O'.