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Bungarus fasciatus antibacterial peptide cathelicidin-BF, and genes and uses thereof

The technology of antibacterial peptide of golden ring snake and golden ring snake, which is applied in the field of biomedicine, can solve the problem of no report on antibacterial peptide research, and achieve the effects of strong killing effect, rapid action time and strong bactericidal effect.

Inactive Publication Date: 2009-04-22
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are a large number of reports on the venom protein of kraitus, various proteases and inhibitors, but there is no report on the antimicrobial peptides in kraitus venom

Method used

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  • Bungarus fasciatus antibacterial peptide cathelicidin-BF, and genes and uses thereof
  • Bungarus fasciatus antibacterial peptide cathelicidin-BF, and genes and uses thereof
  • Bungarus fasciatus antibacterial peptide cathelicidin-BF, and genes and uses thereof

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Embodiment Construction

[0039] The substantive content of the present invention is further described below with embodiment, but content of the present invention is not limited thereto.

[0040] Cloning of cathelicidin-BF antimicrobial peptide gene from golden krait:

[0041] I. Extraction of total RNA from the venom glands of golden krait snakes:

[0042] A. The living golden krait was executed by shearing the head, and the venomous glands were taken, weighed, and 300 mg of venomous gland tissue was added to 10 ml of total RNA extraction buffer (Trizol solution, product of GIBCOBRL, USA), and homogenized in a 20 ml glass homogenizer.

[0043] B. Add an equal volume of phenol / chloroform solution, mix vigorously, leave at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, and discard the precipitate.

[0044] C. Add an equal volume of isopropanol to the supernatant, place at room temperature for 10 minutes, centrifuge at 12,000 rpm for 10 minutes at 4°C, wash the precipit...

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Abstract

The invention discloses cathelicidin-BF and a gene and application thereof, which belong to the field of biomedicine. The cathelicidin-BF is straight chain polypeptide and contains thirty amino acid residues, the molecular weight is 3,637.54Da, and the isoelectric point is 11.79. The complete sequence of the cathelicidin-BF is lysine-phenyl alanine-phenyl alanine-arginine-lysine-leucine- lysine-lysine-serine-valine-lysine-lysine-arginine-lactamine-lysine-glutamic acid-phenyl alanine- phenyl alanine-lysine-lysine-proline-arginine-valine-isoleucine-glycin-valine-serine-isoleucine- praline-phenyl alanine. The gene for encoding the cathelicidin-BF consists of 750 ribonucleotides, wherein 484th to 573rd ribonucleotides are used for encoding a mature peptide part. The cathelicidin-BF has small molecular weight, strong sterilization effect, and quick action time, and has quite strong killing function to a plurality of kinds of clinical drug-fast bacteria. In addition, the cathelicidin-BF also has the advantages of broad-spectrum antibiotics, salt independence and so on.

Description

Technical field: [0001] The invention provides a Bungarus fasciatus antibacterial peptide cathelicidin-BF and its gene and application, belonging to the technical field of biomedicine. Background technique: [0002] Cathelicidins are a family of multifunctional antimicrobial peptides that are currently only found in mammals, birds and fish. Cathelicidin has broad-spectrum antibacterial activity, not only has very strong bactericidal activity against Gram-positive bacteria, Gram-negative bacteria, some fungi and viruses, but also has effects on many clinical drug-resistant bacteria. In addition, cathelicidin also has many other biological activities, such as chemotaxis to various immune cells, inducing mast cell degranulation and histamine release, regulating macrophage transcription, promoting wound healing, inducing angiogenesis, inducing mutation Cell line apoptosis and lymphocyte activation, etc. Because of so many activities, cathelicidin has been a research hotspot in...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/10
Inventor 赖仞王义鹏杨海龙
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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