The present invention discloses a method for splicing first-generation
sequencing data for 16S rDNA identification. It includes: establishing a sequence splicing program based on an alignment module and a splicing module, realizing the splicing of sequence data to be analyzed under the first-generation sequencing
machine, and outputting a
complete sequence that can be used for subsequent identification and analysis; the alignment module includes an alignment model, a scoring standard and a preferred standard; the alignment model uses one direction for scoring calculation and
backtracking; the scoring standard introduces the concept of degenerate bases, and expands 4 bases to 15 bases, including A, T, C, G, R, Y, M, K, S, W, H, B, V, D, N; wherein, when the completely identical bases and degenerate bases contain normal bases, the
score is 10 points, any base alignment N is 1 point, any base alignment "-" is 2 points, and two bases in the same site are different and are 2 points. The present invention establishes the core idea of brand-new sequence splicing, with the advantages of
fast speed, small amount of calculation, and high accuracy.