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Transgenic rice TT51-1 transformation event foreign vector integration site complete sequence and use thereof

A technology for transgenic rice and transformation events, applied in the field of full sequence of exogenous vector integration sites of transgenic rice TT51-1 transformation events, can solve problems that have not yet been discovered

Inactive Publication Date: 2012-04-25
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] After searching the existing patents and other documents, no information about the complete sequence of the transgenic rice TT51-1 event exogenous insertion vector and the flanking sequences on both sides and the establishment of event-specific qualitative and quantitative PCR (polymerase chain reaction) using this sequence have been found. reaction) detection report

Method used

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  • Transgenic rice TT51-1 transformation event foreign vector integration site complete sequence and use thereof
  • Transgenic rice TT51-1 transformation event foreign vector integration site complete sequence and use thereof
  • Transgenic rice TT51-1 transformation event foreign vector integration site complete sequence and use thereof

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Experimental program
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Embodiment Construction

[0046] 1. PCR amplification of the full sequence of the exogenous vector integration site in the transgenic rice TT51-1 transformation event

[0047] First preheat 20% SDS to 65°C, take 15ml SDS extraction buffer (0.1TrisHCl, 0.05MEDTA, 1M NaCl pH8.0) and add it to a 50ml centrifuge tube, then add 2.5μl β-mercaptoethanol and mix well; liquid nitrogen Grind about 3g of leaves, transfer the powder to 50ml of a 50ml centrifuge tube containing extraction buffer, shake and mix on a shaker, add 2ml of preheated 20% SDS, mix well, and bathe in 65°C water for at least 30 minutes, during which time Shake the test tube gently; after the water bath, quickly place the centrifuge tube on ice, add 3ml 3M KAc, mix well, and place it on ice for 30 minutes; centrifuge at 5000g at 4°C for 5min; transfer the supernatant to a new 50ml centrifuge tube, Add 2 / 3 volume of isopropanol, mix well, place at -20°C for more than 30min; centrifuge at 6000g, 4°C for 15min, pour off the supernatant, wash wit...

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Abstract

The invention discloses an event whole sequence with an integration site of a foreign vector transferred by transgenic paddy rice TT51-1 and application of the event whole sequence, relating to safety evaluation and detection of transgenic paddy rice in the biological engineering technical field. The invention uses transgenic insect-resistant rice variety TT51-1 as a material, extracts a gene group DNA as a template, and divides into four sections to enlarge TT51-1 event whole sequence containing a side paddy rice gene group sequence which is shown in SEQ NO.1, and the whole sequence is formed commonly by a basic group of between first and 673rd from the paddy rice gene group, a basic group of between 674th and 9364th from a vector sequence and a basic group of between 9365th and 10536th from the paddy rice gene group; the whole sequence is applicable to carrying out detection, monitoring and safety management of the transgenic paddy rice TT51-1(including parent strain, hybridism F1 and later generation) and products (including plant, tissue, paddy, rice and products thereof).

Description

technical field [0001] The invention relates to the safety assessment and detection of transgenic rice in the technical field of bioengineering, in particular to a complete sequence of an exogenous carrier integration site for a transgenic rice TT51-1 transformation event and its application. Background technique [0002] In recent years, genetically modified crops such as corn, soybean, cotton, rapeseed and tomato have been approved to be planted and produced in many countries, and some have been processed into food, feed or used as food additives. Since the ecological safety and food safety of genetically modified products have been controversial, more than 30 countries and regions have successively implemented genetically modified product labeling systems. [0003] The sequence information of the transformation vector, coding gene sequence, recombination method, insertion site and other sequence information contained in the transformation event is the basis for the safety...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 卢长明吴刚武玉花肖玲曹应龙李均
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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