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16394 results about "Oryza sativa" patented technology
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Oryza sativa, commonly known as Asian rice, is the plant species most commonly referred to in English as rice. Oryza sativa is a grass with a genome consisting of 430Mb across 12 chromosomes. It is renowned for being easy to genetically modify, and is a model organism for cereal biology.
Herbicide tolerant rice plants and methods for identifying same
ActiveUS20080289060A1Superior agronomic phenotypeImprove scalabilitySugar derivativesMicrobiological testing/measurementRice plantsGenetically modified rice
The invention provides specific transgenic rice plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the rice genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.
Owner:BAYER CROPSCIENCE NV
Genomic plant sequences and uses thereof
The present invention discloses rice genomic promoter sequences. The promoters are particularly suited for use in rice and other cereal crops. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the rice genomic promoter sequences, and methods for preparing and using the same.
Owner:MONSANTO TECH LLC
Crop plant cystatin proteinase inhibitors encoding nucleic acids and methods of use
Owner:PIONEER HI BRED INT INC
Rice whole genome SNP chip and application thereof
ActiveCN102747138AImprove throughputGood repeatabilityNucleotide librariesMicrobiological testing/measurementManufacturing technologyGermplasm
The present invention discloses a rice whole genome SNP chip and an application thereof. A method for preparing the chip comprises: (1) obtaining a first class of probes on a chip, wherein sequencing is performed to obtain a parental genome sequence, resequencing data of other rice varieties in a public database are combined, a Nipponbare genome is adopted as a reference sequence, a MAQ software is adopted to match and analyze all the sequencing data, and finally a SNP marker is screened; (2) obtaining a second class of probes on the chip, wherein a rice function gene is obtained from the public database, sequence difference reflecting gene function is searched, and a SNP / INDEL probe is designed according to the sequence difference; (3) adopting an infinium chip manufacturing technology to produce a SNP chip; and (4) testing accuracy and application efficiency of the chip. The chip of the present invention can be applicable for rice germplasm resource molecule marker fingerprint analysis, seed authenticity detection, filial generation genotyping, and other related researches.
Owner:先正达集团股份有限公司
Pyrazole amide compound containing diphenyl ether, and application and pesticide composition of pyrazole amide compound
ActiveCN104557709AImprove biological activityGood prevention effectBiocideOrganic chemistryDiphenyl etherActive ingredient
Disclosed are a pyrazole amide compound containing diphenyl ether as represented by formula I and / or formula II, application thereof in the prevention and treatment of rice sheath blight and cucumber powdery mildew, and pesticide composition containing the compound. The pyrazole amide compound of the present invention containing diphenyl ether as represented by formula I and / or formula II, and a bactericide containing the pyrazole amide compound as an active ingredient have good prevention and treatment effects on rice sheath blight and cucumber powdery mildew.
Owner:燕化永乐(乐亭)生物科技有限公司 +1
Herbicide resistant rice
InactiveUS6274796B1Growth inhibitionGood flexibilityBiocideMutant preparationRice plantsAcetohydroxy Acid Synthetase
Rice plants are disclosed with two separate, but synergistic mechanisms for resistance to herbicides that normally inhibit a plant's acetohydroxyacid synthase (AHAS) enzyme. The herbicide resistance of plants with both resistance mechanisms is substantially greater than one would expect from a simple combination of the two types of resistance. The first of the two resistance mechanisms is a metabolic pathway that is not fully understood, but that does not itself involve a mutant AHAS enzyme. The second resistance mechanism is a mutant AHAS enzyme, an enzyme that shows direct resistance to levels of herbicide that normally inhibit the enzyme, in both in vivo and in vitro assays. Besides controlling red rice, many AHAS-inhibiting herbicides also effectively control other weeds that are common in rice fields. Several of these herbicides have residual activity, so that a treatment controls both existing weeds as well as weeds that sprout later. No herbicide currently available for use on rice has residual activity against a broad spectrum of weeds including red rice. With effective residual activity against red rice and other weeds, rice producers now have a weed control system superior to those currently used.
Owner:BOARD OF SUPERVISORS OF LOUISIANA STATE UNIV & AGRI & MECHANICAL COLLEGE
Rice containing endophytic bacteria and method of producing it
InactiveUS7084331B2Improve the level ofIncrease productionBacteriaUnicellular algaeRice plantsBacteroides
The present invention provides a rice plant that shows enhanced growth and increased seed production, which also enables reduction of the use of chemical fertilizers.In the present invention, a nitrogen-fixing endophytic bacterium is isolated from bacteria symbiotically inhabiting natural plants, the isolated endophytic bacterium is artificially proliferated and then artificially inoculated into rice plants, and thus, the nitrogen-fixing endophytic bacteria are allowed to infect to rice plants.
Owner:SOC FOR TECHNO INNOVATION OF AGRI FORESTRY & FISHERIES +1
Nucleic acid molecules encoding WRINKLED1-like polypeptides and methods of use in plants
InactiveUS8217223B2High oil contentImprove the level ofBryophytesSugar derivativesBiotechnologyFatty acid
Owner:BASF PLANT SCI GMBH
Applications of guaiacol in rice stripe disease
Owner:ANHUI YANGZI CHEM
Breeding method for prolongation of rice fertility stage
ActiveCN104004782AVector-based foreign material introductionAngiosperms/flowering plantsSelf-healingBiotechnology
The invention relates to a breeding method for prolongation of rice fertility stage, and the breeding method comprises the following steps: selecting a target fragment in a rice fertility stage determining gene Ehd3 exon region and constructing a plant CRISPR / Cas9 targeting recombinant vector, introducing into rice cells for regeneration to sprout; using an expression frame in the vector to realize shearing of Ehd3 exon region DNA in the rice cells so as to cause self healing of the rice cells; sequencing regeneration strain genome target fragments to obtain an afunction-mutational strain carrying two equipotential Ehd3 genes, and confirming the prolongation of the regeneration plant fertility stage by phenotypic identification. Experiments show that the method can quickly obtain fertility stage prolonged rice materials.
Owner:RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
Method for planting se-enriched rice
ActiveCN103828665AIncrease productionIncrease selenium contentSeed and root treatmentFertilising methodsBiotechnologyInsect pest
The invention discloses a method for planting se-enriched rice. The method comprises the following steps of land selection and preparation; seeding; seedling culturing; transplanting; field management: intertillage, weeding, topdressing and insect pest prevention; and harvesting. The selenium content of rice planted by using the method is high, and the yield of the rice is also high. Testing confirms that the yield of the se-enriched rice is 620-650kg per acre, and the selenium content of the rice ranges from 0.1 mg / kg to 0.3mg / kg.
Owner:和县金城米业有限责任公司
Bacillus amyloliquefaciens growing in disease-preventing and growth-promoting plant and application thereof
ActiveCN101948771AHas development and application valueImprove efficiencyBiocideBacteriaDiseaseCulture fluid
The invention relates to a bacillus amyloliquefaciens growing in a disease-preventing and growth-promoting plant and application thereof, relating to bacillus amyloliquefaciens and the application thereof. The bacillus amyloliquefaciens TF28 growing in the disease-preventing and growth-promoting plant belongs to bacillus, has the storage number of CGMCC No.4038 and the storage data of July 26, 2010. The application of the bacillus amyloliquefaciens TF28 comprises the following steps of: (1) preparing activated bacilli liquid; (2) inoculating the activated bacilli liquid in an NYD culture fluid to obtain a strain fermentation solution; and (3) soaking seeds in a dilution solution 50 times of the strain fermentation solution. The strain TF28 in the invention can be colonized, reproduced and transmitted in plants, can simultaneously generate two antibacterial substances, i.e. antimicrobial proteins and lipopeptide antibiotic and has the multiple efficacy of preventing diseases, stimulating growth, increasing the yield, improving the quality, widening an antibacterial spectrum, and the like. Rice seeds treated by the dilution solution 50 times of the fermentation solution of the strain TF28 reach a room-temperature bakana resistance rate over 84.6 percent.
Owner:INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
Streptomyces corchorusii strain NF0919, purpose and preparation method of active zymotic fluid thereof
ActiveCN102010835AGood control effectThe fermentation process is simpleBiocideBacteriaDiseasePlant disease
The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.
Owner:ZHENJIANG AGRI SCI INST JIANGSU HILLY AREAS
Gene editing method for knocking out rice MIRNA393b stem-loop sequences with application of CRISPR(clustered regulatory interspersed short palindromic repeat)-Cas9 system
InactiveCN105647962AKnockout worksNucleic acid vectorVector-based foreign material introductionEscherichia coliEnzyme digestion
The invention relates to construction of rice transgenic materials and aims to provide a gene editing method for knocking out rice MIRNA393b stem-loop sequences with application of a CRISPR(clustered regulatory interspersed short palindromic repeat)-Cas9 system. The gene editing method comprises steps as follows: gRNA target sites are selected for cloning and GG linking, enzyme digestion is performed after amplification, and a product is linked with a pGREB 32 vector; escherichia coli competent cells are transformed; plasmids with a correct sequencing result are used for transforming agrobacteria, transgenic plants are obtained through mediated transformation of rice calli, and transgenic positive lines are obtained; the T0-generation mutant plant seeds are collected for seeding, and the T1-generation plants are subjected to homozygote screening; homozygous lines which are discovered to be negative through MIRNA393b expression are rice mutants completely losing the MIRNA393b stem-loop sequences and MIRNA393b stem-loop sequence expression. According to the gene editing method, MIRNA stem-loop sequences can be effectively knocked out, and loss-of-function mutants of different members in the same MIRNA family can be prepared; the mutant plant propagates to obtain a large number of seeds and is an ideal material for acquiring rice MIRNA393b gene functions successfully.
Owner:ZHEJIANG UNIV
Method for comprehensively raising various types of aquatic livestock and cultivating paddy in paddy field
InactiveCN103348890AReduce stocking densityPromote small sizeClimate change adaptationAnimal feeding stuffCarassius auratus gibelioIngested food
The invention relates to a method for comprehensively raising various types of aquatic livestock and cultivating paddy in a paddy field, in particular to the raising of Chinese soft-shelled turtles, crawfish and carassius auratus gibelio in the paddy field and the cultivation of paddy. The method comprises the steps of paddy field preparation, aquatic plant transplantation, live food feeding, paddy transplantation, young aquatic livestock placement, bait feeding, daily management, aquatic livestock fishing and paddy harvest. The method for comprehensively raising various types of aquatic livestock and cultivating paddy in the paddy field has the advantages that the various types of aquatic livestock can be raised and paddy can be cultivated in the same field comprehensively, the difference between the period for paddy cultivation and the period for raising aquatic livestock is small, the use rate and the comprehensive efficiency of the field can be effectively improved, and economic benefit can be improved to a large extent; meanwhile, any chemical fertilizer, herbicide and insecticide are not used in the process of production, the produced products can meet the standard of organic food, and finally, edible safety of the products is achieved; the whole process of production is beneficial to conservation of water and soil of the field and the surrounding environment and effectively facilitates the protection of natural ecology.
Owner:湖北省水产技术推广中心
Method for producing fertilizer with papermaking sewage sludge
InactiveCN101429066ARealize comprehensive utilizationSolve processing problemsBio-organic fraction processingOrganic fertiliser preparationSludgePotassium
The invention discloses a method for preparing organic fertilizer from paper-making sludge. The materials such as paper-making sludge, amendment are mixed, subjected to high-temperature aerobic fermentation, post maturation and crushing, screened, and mixed with fertilizer of nitrogen, phosphorus, potassium and the like and medium and micro elements to prepare the organic fertilizer. The method has the advantages of simple process, easy operation, obvious innocuous effect, no environmental pollution and little nutrient loss; and the organic fertilizer prepared by the method is widely applied to industrial crops such as vegetables, fruit trees, Chinese herbal medicines, flowers and field crops such as wheat, corn, paddy rice.
Owner:SHOUGUANG CAILUN ZHONGKE FERTILIZER
Application of rice transcription factor Os06g08400 genes
The invention relates to an application of rice transcription factor Os06g08400 genes. A transcription factor activation motif VP64, namely four transcription factor activation motifs VP16, is combined with the rice transcription factor Os06g08400 genes to establish a combined transcription factor, and then the combined transcription factor is converted into crops such as rice so that the characters of rice grains are improved, for example, the rice grains are obviously widened and thickened. The application related by the invention has an important theoretical value for detail definitions of a mechanism for regulating and controlling seed development, and the characters of the rice grains can be improved through a transgene means, so that the application related by the invention is also of importance in production practices.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
HERBICIDAL COMPOSITIONS COMPRISING 4-AMINO-3-CHLORO-5-FLUORO-6-(4-CHLORO-2-FLUORO-3-METHOXYPHENYL) PYRIDINE-2-CARBOXYLIC ACID OR A DERIVATIVE THEREOF AND ACETYL-CoA CARBOXYLASE (ACCASE) INHIBITORS
Provided herein are synergistic herbicidal compositions containing and methods utilizing (a) a compound of formula (I):or an agriculturally acceptable salt or ester thereof and (b) an ACCase inhibitors, including. e.g., clethodim, clodinafop-propargyl, cyhalofop-R-butyl, diclofop-methyl, fenoxaprop-P-ethyl, fluazifop-P-butyl, haloxyfop-R-methyl, metamifop, pinoxaden, profoxydim, quizalofop-P-ethyl, sethoxydim and tralkoxydim, provide synergistic weed control of undesirable vegetation in rice, cereals, wheat, barley, oats, rye, sorghum, corn / maize, sugarcane, sunflower, oilseed rape, canola, sugar beet, soybean, cotton, pineapple, pastures, grasslands, rangelands, fallowland, turf, tree and vine orchards, aquatics, plantation crops, vegetables, industrial vegetation management (IVM) or rights of way (ROW).
Owner:CORTEVA AGRISCIENCE LLC
Bacillus belleus GT11 and application thereof
The invention discloses a bacillus belleus GT11 and application thereof. The bacillus belleus CT11 is collected at China General Microbiological Culture Collection Center on December 13th, 2016; the collection number is CGMCC No.13446; the bacillus belleus CT11 can be prepared into water agent, wettable powder or coating material and can be used for preventing and controlling the root rot diseases of pseudo-ginseng and plukenetia volubilis and Chinese cabbage clubroot. The invention provides an efficient, nontoxic, safe, non-residual and conveniently used biological agent for preventing and controlling the diseases such as root rot and clubroot; the effective active ingredient is bacillus belleus CT11 strain; the strain can generate the secondary metabolite antimicrobial active matter, is capable of degrading the cell wall of pathogenic bacteria and has an excellent growth-promoting effect for the crops, such as, plukenetia volubilis, pseudo-ginseng and Chinese cabbage; besides, the bacillus belleus CT11 has a better antibacterial effect for pseudo-ginseng phytophthora sojae, pseudo-ginseng fusarium solani, plukenetia volubilis gray mold, paddy bacterial mycosphaerella, and the like.
Owner:YUNNAN AGRICULTURAL UNIVERSITY
Application of CRISPR/nCas9 mediated site-directed base substitution in plant
ActiveCN107043779ARapid improvement of agronomic traitsImproved agronomic traitsVectorsVector-based foreign material introductionUracil-DNA glycosylaseSubstitution method
The invention discloses application of CRISPR / nCas9 mediated site-directed base substitution in a plant. The invention provides a plant genome site-directed edition system. The system comprises a BE3 plant expression carrier (expressing a fusion protein composed of nCas9(D10A), deaminase and a uracil DNA glycosylase inhibitory protein), and rice OsPDS and OsSBEIIb are taken as target genes for verifying the system. Results show that an expected site-directed mutant plant is respectively obtained in the three selected target spots, accurate site mutation of a base is realized in rice, and the highest efficiency reaches about 20%, so that a feasible and effective base substitution method is provided for crop breeding, the method has strong application potential in the aspect of agricultural breeding, and a foundation is provided for rapidly improving important agronomic traits of crops.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
CRISPR/Cpf1 plant genome directional modification function unit, carrier comprising function unit, and application thereof
ActiveCN107012164AEfficient constructionDirectional modification shortcutVector-based foreign material introductionGene expressionNuclease
The invention belongs to the technical field of gene engineering, and concretely relates to a CRISPR / Cpf1 plant genome directional modification function unit, a carrier comprising the function unit, and application thereof. The invention aims to build an effective plant genome CRISPR / Cpf1 directional modification framework carrier according to an action principle of a CRISPR / Cpf1 system and a vegetable cell gene expression characteristic, and realize high-efficient application thereof in plant genome directional modification. The technical scheme provided by the invention is characterized by building the CRISPR / Cpf1 plant genome directional modification framework carrier formed by lachnospiraceae bacterium Cpf1 nuclease protein (LbCpf1) expression unit and a guiding crRNA transcription expression unit. The invention also provides a method for designing specificity-guided crRNA and building a CRISPR / Cpf1 recombinant expression vector aiming at rice genome target loci by adopting the CRISPR / Cpf1 plant genome directional modification framework carrier. The invention provides the high-efficient CRISPR / Cpf1 plant directional modification framework carrier, which can effectively realize simple, fast and high-efficient directional modification based on the CRISPR / Cpf1 system aiming at a plant genome target sequence.
Owner:UNIV OF ELECTRONIC SCI & TECH OF CHINA
Production of recombinant human serum albumin with rice-embryo milk cell as biological reactor
ActiveCN1896239ANo pollution in the processEfficient expressionSerum albuminFermentationSerum protein albuminEmbryo
Production of recombinant human serum albumin with rice albuminous cell as biological reactor is carried out by taking rice albuminous cell protein as storage site for recombinant protein, taking rice albuminous specific expression starter and signal peptide, entering inductive recombinant human serum albumin into inner-film system of rice serum albumin cell and storing it in rice albuminous protein. It is cheap, has more yield and higher expression.
Owner:WUHAN HEALTHGEN BIOTECHNOLOGY CORP
Machine-transplanted rice seedling raising ground substance and production method thereof
ActiveCN101507410AGood qualityNo soreBio-organic fraction processingOrganic fertiliser preparationMicroorganismCaking
The invention relates to a rice seedling raising substrate and a production method thereof, in particular to a rice seedling raising substrate taking agricultural and forestal wastes as materials and mechanically transplanting rice seedlings, and a production method thereof. In the invention, organic wastes such as straws are taken as raw materials, fermented by various beneficial microorganisms, and prepared into an organic substrate with a fertilizer slow release and caking agent and a water retention agent. The rice seedling raising substrate has strong stress resistance and strong compatibility with mechanical rice transplanting technology, and makes the rice seedlings have good quality; and the technology of raising the rice seedlings by the substrate is simple, and has low cost and high success ratio.
Owner:HUNAN CHAIMIHE AGRI TECH DEV
Method for cultivating low-cadmium-accumulation indica rice variety
ActiveCN106544357AAvoid safety hazardsPrecise and controllable mutation sitesNucleic acid vectorPlant peptidesTransgenesisMutant
The invention discloses a method for cultivating a low-cadmium-accumulation indica rice variety. The method includes the steps of cloning part of the CDS sequence of OsNramp5 of an indica rice acceptor material; using a CRISPR / Cas9 system to select a target sequence according to an exon sequence; building pCRISPR / Cas9 recombinant vectors; leading the pCRISPR / Cas9 recombinant vectors into rice calluses to obtain transgene seedlings; screening transgene positive plants; obtaining mutant plants; and conducting seed propagation of the mutant plants, and separating afunction mutants containing no transgenic ingredients from plant progenies. By means of the method, the cadmium absorption major gene OsNramp5 of indica rice is directionally knocked out through the CRISPR / Cas9 technology, and the indica rice material containing no transgenic ingredients, having no significantly variant comprehensive agronomic characters and achieving a low cadmium content in rice is directionally cultivated. The method has the advantages that targeting is efficient, the breeding period is short, cost is low and practicality is high.
Owner:HUNAN HYBRID RICE RES CENT
Method for estimating aboveground biomass of rice based on multi-spectral images of unmanned aerial vehicle
ActiveUS20200141877A1Low input data requirementImprove estimation accuracyImage enhancementInvestigation of vegetal materialMultivariate linear modelVegetation Index
A method for estimating the aboveground biomass of rice based on multi-spectral images of an unmanned aerial vehicle (UAV), including: normatively collecting UAV multi-spectral image data of rice canopy and ground measured biomass data; after collection, preprocessing images, extracting reflectivity and texture feature parameters, calculating a vegetation index, and constructing a new texture index; and by stepwise multiple regression analysis, integrating the vegetation index and the texture index to estimate rice biomass, and establishing a multivariate linear model for estimating biomass. A new estimation model is verified for accuracy by a cross-validation method. The method has high estimation accuracy and less requirements on input data, and is suitable for the whole growth period of rice. Estimating rice biomass by integrating UAV spectrum and texture information is proposed for the first time, and can be widely used for monitoring crop growth by UAV remote sensing.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for transforming rice into fragrant rice rapidly
InactiveCN105543228AGenetic stabilityRapid homozygousOxidoreductasesFermentationSelfingGene silencing
The invention discloses a method for transforming rice into fragrant rice rapidly. By means of the CRISPR / Case technology and related gene sequences in the rice fragrance metabolic process, a carrier is designed at the specific site, the element is transferred to non-fragrant rice through agrobacterium-mediated transformation, the gene is made to cause deletion mutation at the position of a fixed point, the gene is silent, fragrance cannot be normally metabolized and greatly accumulated, common rice is transformed into fragrant rice, then genetic transformation gene segments are separated from edited genes through selfing or hybridization, and the fragrant rice with the isozygoty capable of being stably inherited is rapidly obtained.
Owner:NINGXIA ACADEMY OF AGRI & FORESTRY SCI
Rice field-fishpond planting and breeding compound system
ActiveCN103437577ARegulate water qualityImprove growth performanceAgricultural buildingsPisciculture and aquariaCompound systemPaddy field
The invention relates to a rice field-fishpond planting and breeding compound system, which belongs to the technical field of comprehensive utilization of agricultural ecological aquaculture. The compound system comprises a rice field, a fishpond, a simple ecological ditch and a strengthened ecological ditch, wherein the rice field is arranged at one end of the fishpond; the strengthened ecological ditch and a water collecting tank are respectively arranged on one side of each of the fishpond and the rice field; the simple ecological ditch is formed in the rice field; a small overflow port is formed in the simple ecological ditch; the rice field is communicated with the water collecting tank by a large overflow port; the fishpond is communicated with the simple ecological ditch by a water suction pump. According to the rice field-fishpond planting and breeding compound system disclosed by the invention, the rice field planting and the fishpond breeding are organically combined by the ecological ditches, so that the fishpond breeding water is refluxed to a new circulating water planting-breeding mode after being purified by the rice field and the ecological ditch. Moreover, the problems that an existing rice-orientated planting-breeding mode with the fish breeding has rice-fish contradictions including field drying, fertilizer applying, pest controlling and the like in the production process, and is limited in yield and inconvenient for large-area popularization are solved.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
Application for using LbCpf1-RR mutant in CRISPR/Cpf1 system in plant gene editing
ActiveCN108486146AExpand the scope of editingAntibody mimetics/scaffoldsNucleic acid vectorRice plantsMutant
The invention discloses application for using an LbCpf1 - RR mutant in a CRISPR / Cpf1 system in plant gene editing. The application for using the LbCpf1 - RR mutant in the CRISPR / Cpf1 system in plant gene editing uses an OsPDS gene and an OsSBEIIb gene as target genes, constructs a target double loci of one gene and a series of vectors of two genes, and utilizes an agrobacterium-mediated transformation method to import the vectors into rice callus, and rice plants with the target gene knockout are successfully obtained by using the LbCpf1-RR mutant. The only difference between the LbCpf1-RR mutant and the protein LbCpf1 is that the 532nd amino acid changes from G to R, and the 595th amino acid changes from K to R. The LbCpf1-RR mutant provided by the application for using the LbCpf1 - RR mutant in the CRISPR / Cpf1 system in plant gene editing expands the PAM site sequence identified by the LbCpf1-RR mutant, so that the editing range of the CRISPR / Cpf1 system in rice genome is expanded, great significance for promoting the application of the system in the field of plant genome editing is achieved. The application for using the LbCpf1 - RR mutant in the CRISPR / Cpf1 system in plant geneediting has great application value.
Owner:INST OF CROP SCI CHINESE ACAD OF AGRI SCI
RCc3 regulatory elements for use in plants
The present invention provides polynucleotide molecules isolated from Oryza sativa and useful for expressing transgenes in plants. The present invention also provides DNA constructs containing the polynucleotide molecules useful for expressing transgenes in plants. The present invention also provides transgenic plants and seeds containing the polynucleotide molecules useful for expressing transgenes in plants.
Owner:MONSANTO TECH LLC
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