2793 results about "Oryza sativa" patented technology

The present application relates to a kind of deletion mutant nucleic acid and its application in anti-herbicide.The deletion mutation occurs in the promoter of rice OsHPPD Gene, the length of deletion sequence is at least 10 bp, and the A in the start codon ATG of gene is 0, at least the bases located in the upstream of start codon ATG from 2041 to 2032 are deleted. OsHPPD The present application finds that by deleting part of the sequence in the promoter of rice OsHPPD Gene, rice can obtain resistance to HPPD inhibitor herbicides, which is of great application value for ensuring agricultural production safety and improving the efficiency of herbicide use.

The invention relates to a rice identification method based on optical and SAR image fusion, and belongs to the technical field of remote sensing image processing. The method comprises the following steps: synchronously acquiring SAR and optical time sequence data, respectively preprocessing the SAR and optical time sequence data, and extracting characteristic parameters; the method comprises the following steps: constructing a double-flow Vision Transform model, wherein the double-flow Vision Transform model comprises a double-branch feature extraction module, a space-time Transform aggregation module, a gating feature fusion module and a decoder; the double-branch feature extraction module extracts SAR and optical features according to the SAR and optical feature parameters; inputting the extracted SAR features and optical features into a space-time Transform aggregation module, and carrying out time sequence dependence modeling through a self-attention mechanism; the gating feature fusion module performs weighted fusion on the optical and SAR features; and the decoder generates a rice distribution probability graph according to a weighted fusion result. According to the method, the rice identification precision under the multi-temporal and multi-environment conditions can be improved.

The invention discloses a multi-platform real-time field rice ear detection and counting method based on a visual converter, which is suitable for various acquisition platforms from a portable mobile terminal to an unmanned aerial vehicle terminal and the like, and meets the requirements of small-range accurate identification and large-range high-throughput monitoring. Aiming at the characteristics of multiple scales, multiple varieties, phenotype difference, time sequence change, complex field environment and the like in a data set, a rice spike detection counting network comprising a feature enhancement module, an efficient cross-space feature extraction network, a lightweight bidirectional feature fusion module and a number estimation and detection head module is constructed. The average precision mean value (mAP50) of the model reaches 0.8906 and is improved by 11.5% compared with that of an original model, the parameter quantity is reduced by 29%, and the calculated quantity (GFLOPs) is reduced by 48%. The method can effectively process rice ear images of different scales and growth periods, adapts to morphological change, overcomes interference such as illumination, phenotypic difference and shielding, and provides technical support for rice yield evaluation and field rice ear quality analysis.

The invention discloses a compound microbial agent for reducing the cadmium content of brown rice and application of the compound microbial agent. Belongs to the technical field of agricultural microorganisms. The compound microbial agent is prepared from herbaceous spirillum, stenotrophomonas and coxakia, the herbaceous spirillum is herbaceous spirillum R3, and the preservation number of the herbaceous spirillum is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the stenotrophomonas is stenotrophomonas R5, and the preservation number of the stenotrophomonas is CCTCC (China Center For Type Culture Collection) NO: M 20231758; the coxakia is coxakia P1-4, and the preservation number of the coxakia is CCTCC (China Center For Type Culture Collection) NO: M 20231759. Compared with the prior art, the compound microbial agent disclosed by the invention has the beneficial effects that the compound microbial agent disclosed by the invention can reduce the content of cadmium in brown rice, so that farmland soil polluted by light and medium cadmium can be repaired and produced at the same time, and the edible safety of agricultural product rice is ensured.

The invention belongs to the technical field of rice panicle shielding image data processing, and provides a rice panicle shielding image restoration method and system based on a generative adversarial network, and the method comprises the steps: collecting rice panicle images at different angles and under different illumination conditions to construct a data set, employing a target detection model to carry out the positioning of a rice panicle region, and classifying the shielding types, and extracting a visible area of the rice spike by adopting a semantic segmentation network, and repairing a sheltered area under a generative adversarial network framework to obtain a complete rice spike image. The method is suitable for complex scenes such as leaf shielding, inter-panicle mutual shielding and mixed shielding, texture details and structure consistency of the repaired image can be guaranteed, acquisition of complete phenotype information of the rice panicles is achieved, and reliable data support is provided for rice yield estimation and precision agricultural management.

The invention relates to the field of artificial intelligence, in particular to a rice growth vigor dynamic monitoring method and system based on modal fusion and deep learning. The method comprises the following steps: for a plurality of target regions in the same administrative division, acquiring multi-dimensional agricultural data related to rice growth vigor in each target region; performing space-time fusion on the multi-dimensional agricultural data of the plurality of target areas to obtain a linkage monitoring map; performing rice growth prediction on each target area in the linkage monitoring map through a DSW Transform model to obtain an independent growth prediction result of each target area; a correlation weight is dynamically calculated through a deep learning model of a cross-modal attention mechanism in combination with geographic data, meteorological data and a linkage monitoring map, and rice growth prediction is performed on a rice block formed by linkage of each target area based on the correlation weight to obtain a comprehensive growth prediction result; and pushing rice growth information in each target area to the user according to the independent growth prediction result and the comprehensive growth prediction result.

The invention provides a rice variable rate fertilization method and device, electronic equipment and a storage medium, and relates to the technical field of agricultural intelligent information process.The method comprises the steps that non-rice plant interferents in a multispectral remote sensing image are removed, and a rice plant canopy mask is generated; based on the rice plant canopy mask, multi-dimensional features of the rice plant canopy are extracted and fused, and a fused growth feature vector is obtained; grading the rice growth vigor according to the fused growth vigor feature vector through a growth vigor grade prediction model to obtain a predicted growth vigor grade; performing standardization processing on the predicted growth vigor level to obtain a standardized growth vigor level; determining a nitrogen fertilizer adjustment factor based on the standardized growth vigor grade; and according to the nitrogen fertilizer adjustment factor, adjusting a standard nitrogen application amount to obtain a recommended nitrogen application amount. According to the invention, accurate recommendation of the nitrogen application amount in the rice planting process can be realized, so that accurate fertilization is realized.

The invention discloses a microbial agent and application thereof in preventing and treating rice blast and promoting rice growth, active ingredients of the microbial agent are formed by compounding cold bacillus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) NO: 33492 and bacillus velezensis with the preservation number of CGMCC NO: 33493, and the microbial agent aims at solving the synergistic problem of preventing and treating rice blast and promoting rice growth. According to the complex microbial inoculant, through the synergistic interaction between the two strains, the metabolite of the complex microbial inoculant can completely inhibit the spore germination of magnaporthe oryzae; meanwhile, the fungicide can degrade cellulose, obviously promote the growth of plant height, fresh weight and dry weight of rice, and realize the integration of disease prevention and growth promotion. The invention further discloses a preparation method of the fungicide and an application method of the fungicide for preventing and treating rice blast and promoting growth in a root irrigation mode in the three-leaf stage of rice. The microbial agent disclosed by the invention has dual functions of efficiently preventing diseases and stably promoting growth, and an efficient and safe biological solution is provided for green and sustainable development of agriculture.

The invention relates to the technical field of biological breeding, in particular to a rice hybrid combination optimization method based on an adaptive algorithm, and aims to solve the problems that existing rice breeding is poor in adaptability, multi-objective optimization is difficult and artificial experience is limited. The method is characterized in that a data integration module, a dynamic target module, a parent library module, a genetic prediction module, a self-adaptive optimization module (including a genetic algorithm and reinforcement learning), a simulation evaluation module and a recommendation feedback module are constructed, and precise prediction and dynamic optimization of hybrid combination are achieved. By adopting the scheme, the limitation of artificial experience is effectively overcome, the breeding decision is changed from static experience to dynamic data drive, and the breeding efficiency, accuracy and adaptability are remarkably improved.

The invention discloses a rice growth period distinguishing method and an intelligent irrigation control device. Belongs to the technical field of crossing of intelligent agriculture and irrigation and water conservancy engineering and particularly relates to the technical field of rice growth period judgment and intelligent irrigation. The technical problem that an accurate and effective rice growth period judgment method for guiding intelligent rice irrigation is lacked at present is solved. The method specifically comprises the following steps: calculating a rice growth process index through an improved accumulated temperature model; and constructing a multi-factor coupling growth period judgment method, and judging the current rice growth period through the rice growth process index. The system comprises a sensing unit, a judgment and decision-making unit, an electric execution unit and an input and power supply unit, the judgment and decision-making unit receives the environment data of the rice field and the water layer depth data of the rice field, judges the current growth period of the rice according to a rice growth period judgment method, calculates the current water layer deviation and generates a control instruction to the electric execution unit.

The invention discloses pseudomonas chlororaphis S7-24 for antagonizing xanthomonas oryzae and application of the pseudomonas chlororaphis S7-24, and belongs to the technical field of microorganisms. The pseudomonas chlororaphis S7-24 is preserved in the China Center for Type Culture Collection on November 12, 2025, and the preservation number of the pseudomonas chlororaphis S7-24 is CCTCC NO: M 20252533. The pseudomonas chlororaphis S7-24 provided by the invention is a newly found pseudomonas biocontrol resource, and verification shows that the strain S7-24 has relatively high antagonistic activity on rice bacterial blight and rice bacterial streak, and can be used for effectively preventing and treating rice bacterial blight and rice bacterial streak. The pseudomonas chlororaphis S7-24 provided by the invention is used for preventing and treating rice bacterial leaf blight and rice bacterial leaf streak, can reduce the use amount of chemical pesticides, ensures grain safety, and has better economic and ecological benefits.

The invention relates to the field of rice gene engineering, and discloses an application of a GSE3.1 protein and a coding gene thereof in regulating and controlling the grain length, the grain width and the grain weight of rice seeds. According to the invention, a GSE3.1 gene is edited based on a CRISPR / Cas9 technology, a japonica rice variety Zhonghua 11 (ZH11) is introduced by using an agrobacterium-mediated method, and a knockout mutant is obtained through screening. The homozygous knockout mutation of the GSE3.1 gene in the invention leads to reduction of the grain width and grain length of rice seeds and reduction of thousand grain weight. According to the invention, the constructed plant overexpression vector ProActin: GSE3.1 is expressed in wild type ZH11, so that compared with the wild type plant, the seed grain length and grain width of the transgenic plant are obviously increased, and the thousand grain weight is obviously increased. Therefore, the GSE3.1 gene and the encoding protein thereof can regulate and control the size and the weight of the rice seeds, and are of great significance to cultivation of high-yield rice varieties.

The invention discloses application of an upstream non-coding region uORF of an ABA2 gene in regulation and control of crop agronomic traits, in particular to application in improvement of pre-harvest sprouting resistance of rice and cultivation of pre-harvest sprouting resistant rice strains, and belongs to the technical field of gene engineering. The invention finds that uORF in the upstream non-coding region of the crop ABA2 gene can inhibit the translation level of downstream ABA2 protein on the basis of not influencing the transcriptional level, so that the uORF can participate in regulation and control of crop agronomic traits in which ABA participates in regulation and control, such as improvement of pre-harvest sprouting resistance of rice. Therefore, the upstream non-coding region uORF of the crop ABA2 gene can be modified through a hybridization or gene editing technology, so that the purpose of regulating and controlling crop agronomic traits in which ABA participates in regulation and control is achieved, for example, site-directed mutagenesis is performed on an initiation codon of the upstream non-coding region uORF of the rice ABA2 gene, and the pre-harvest sprouting resistance of rice is improved.

Aiming at the problems of low efficiency, strong subjectivity, insufficient modeling ability, complex hyperspectral data noise, weak waxiness spectrum difference and the like of a traditional rice grain waxiness discrimination method, the invention provides a hyperspectral rice grain waxiness discrimination method based on multi-branch collaborative modeling. The method comprises the following steps: S1, acquiring glutinous and non-glutinous rice grain images by using a hyperspectral imaging system to obtain spectral data; s2, preprocessing spectral data through a combined method of SG smoothing, an asymmetric weighted penalty least square method and multivariate scatter correction to reduce noise and interference; s3, constructing a multi-branch modeling architecture which comprises a CNN local feature extraction module, an SRU spectrum sequence modeling module and a HorNet global high-order modeling module, and outputting a discrimination result through a classifier after multi-branch features are subjected to fusion and pooling attention weighting; and S4, evaluating the performance. According to the method, high-precision lossless discrimination of the waxiness is realized, and a technical support is provided for germplasm screening and quality evaluation in rice breeding.

The invention relates to the field of soil nutrient management, in particular to a rice soil nutrient coordinated regulation and control method and system based on big data analysis, and the method comprises the steps: firstly, collecting data through a multi-source sensing array, and constructing a feature state space with time sequence memory; a physical field theory is introduced, and the buffer effect of soil on nutrients is modeled into a chemical potential energy field by utilizing a variety Hooke's law, so that the system instability is quantified, and the problem of excessive fertilization caused by a hysteresis effect is solved. In the aspect of control decision, rolling horizon optimization is adopted, the potential energy burden serves as a penalty term to be introduced into a genetic algorithm, and blind topdressing under model mismatch is prevented by adaptively correcting and searching a spatial constraint boundary through a predicted residual error of a previous period. And finally, a smoothing instruction is generated through low-pass filtering, and accurate and steady regulation and control of rice soil nutrients are realized.

The invention belongs to the field of plant seed biology and rice breeding, and discloses an application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of storage resistance of rice seeds and a related breeding method. In particular discloses application of an OsBTB05 gene or an OsBTB05 protein in regulation and control of the storability of rice seeds, the nucleotide sequence of the OsBTB05 gene is shown as SEQ ID NO: 1 or SEQ ID NO: 2, and the amino acid sequence of the OsBTB05 protein is shown as SEQ ID NO: 3. The invention also discloses a molecular marker-assisted selection method for breeding the high-storage-resistance rice variety and a method for reducing the storage resistance of the rice seeds. The invention proves that the OsBTB05 gene is a key factor for regulating and controlling the storability of the rice seeds, and the expression level of the OsBTB05 gene is in significant positive correlation with the storability of the seeds. A clear molecular target and a brand-new gene resource are provided for improving the seed storage resistance from a genetic source.

The invention relates to the technical field of gene editing, and particularly provides a method for creating herbicide-resistant rice through gene editing of an OsEPSPS gene promoter region, and the method is characterized in that WT / 29bp single allelic deletion mutation is introduced into the OsEPSPS gene promoter region, i.e., A in an OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, A in the OsEPSPS gene initiation codon ATG is 0 site, and A in the OsEPSPS gene initiation codon ATG is 0 site; 29bp is deleted from the 112nd site to the 1150th site at the upstream of the initiation codon ATG on one allele, and the other allele is kept unchanged. By introducing the single allelic deletion mutation, the rice can obtain resistance to EPSPS inhibitor herbicides, the resistance is identified in T0-generation plants carrying the mutation and can be stably inherited to offspring plants, and the application value is high for guaranteeing agricultural production safety and improving the use efficiency of the herbicides.

The invention relates to the technical field of biology, in particular to application of a rice OsPBL8 gene in enhancing resistance of rice to magnaporthe oryzae. The rice OsPBL8 gene is a rice endogenous gene participating in the processes of plant growth and development, abiotic stress response, hormone signal channel regulation and control and the like. The invention provides application of the OsPBL8 gene in the field of disease resistance, the OsPBL8 gene is overexpressed through gene editing, and the expression quantity of receptor-like cytoplasm kinase OsPBL8 in plants is increased, so that a plurality of signal channels are regulated and controlled, and the germ resistance of the plants is synergistically improved. By adjusting key hub nodes of plant targeted immune signal transduction, more lasting broad-spectrum disease resistance can be provided, good universality is achieved, disease resistance and growth can be balanced, good practicability is achieved, and improvement of disease resistance of various rice varieties is facilitated.

The invention discloses a method for reducing cadmium accumulation of rice and improving salt tolerance. According to the invention, the CDS sequence expression of the OsRBOHA is driven by the OsNAC4 promoter, so that the avoidance capability of the rice to non-uniform stress is remarkably improved, and the stress exposure of a root system is reduced. A soil culture experiment shows that the pOsNAC4: OsRBOHA strain reduces cadmium accumulation of overground parts and grains in non-uniform cadmium polluted soil, and enhances salt tolerance of the overground parts in non-uniform salt stress soil.

The invention belongs to the technical field of agricultural microorganisms, and discloses a strain of stenotrophomonas sp., which is named as stenotrophomonas sp. R5, is preserved in the China Center for Type Culture Collection on September 20, 2023, the preservation address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 20231758. The invention further discloses a microbial agent containing the bacterial strain and application of the microbial agent in prevention and treatment of rice planthoppers. According to the stenotrophomonas sp. R5 and the microbial agent containing the stenotrophomonas sp. R5, the control effect on rice planthoppers can reach 90% or above, compared with a conventional control method, the control effect is remarkably improved, and the survival rate of rice is greatly increased.

The invention discloses a grain specific promoter in the early stage of rice filling and application thereof, the promoter is named as proASP1, and the nucleotide sequence of the promoter is shown as SEQ ID NO: 1. The promoter has highly strict spatio-temporal expression specificity, the activation expression of the promoter is only limited in grains in the early stage of rice filling (4-12 days after flowering), especially the expression of genes is efficiently driven in endosperm and aleurone layers, and the expression is not leaked in other tissues such as roots, stems, leaves, leaf sheaths and glumes and grains in the middle and later stages of filling. The invention provides a basic and precious molecular tool for gene function research, quality improvement (such as protein and starch synthesis regulation) and bioreactor development in a key window period of rice grain development, and has important scientific research and application values.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The invention provides a closely linked molecular marker primer of a rice brown planthopper resistant major gene Bph56 as well as a marking method and application of the closely linked molecular marker primer. According to the invention, genotypes of F2 individual plants obtained by hybridizing a rice insect-resistant variety C127 (or) and KW (or) are combined with brown planthopper resistance levels of an F3 family for genetic linkage analysis, a resistance major gene Bph56 carried by the insect-resistant variety C127 is identified, and a region of the resistance major gene Bph56 is reduced to a 77kb fragment limited by markers 6M26.278 and 6M26.355 on two sides and is closely linked with a molecular marker 6M26.355. The molecular marker 6M26.355 disclosed by the invention can be used for effectively detecting whether an insect-resistant variety C127 and derived varieties (lines) of the insect-resistant variety C127 contain the major resistance gene locus or not, so that the selection efficiency of the brown planthopper-resistant character is greatly improved, and the brown planthopper-resistant rice variety (line) containing Bph56 is obtained.

The invention belongs to the technical field of genetic breeding, and particularly relates to a KASP primer group for detecting the typing of a rice flowering time regulation gene OsMYB8 and application of the KASP primer group. The KASP primer group comprises a first forward primer, a second forward primer and a reverse primer; the nucleotide sequence of the first forward primer is as shown in SEQ ID NO. 10; the nucleotide sequence of the second forward primer is as shown in SEQ ID NO. 11; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 12. The primer group disclosed by the invention can be used for rapidly and accurately identifying the genotype of the rice OsMYB8 gene, and can be applied to molecular marker-assisted breeding of OsMYB8 gene plants.

The invention discloses streptomyces griseorubens and an application of the streptomyces griseorubens in phthalate pollution remediation. The bacterial strain is Streptomyces griseorubens FZ202, the preservation number of the bacterial strain is GDMCC No: 61372, the bacterial strain has good environmental adaptability and efficient degradation capacity, DEHP can be effectively degraded within 100-800 mg / L, the highest degradation efficiency can reach 94.1%, and complete degradation can be achieved. The strain is inoculated into PAEs contaminated soil and interacts with rice, DEHP residues in the soil and crop bodies can be effectively reduced, various PAEs can be synergistically degraded, and the strain has the broad-spectrum degradation characteristic. Meanwhile, through multiple mechanisms of degrading pollutants, promoting plant growth, improving the soil environment and regulating and controlling microbial communities, the strain remarkably improves the growth performance of rice and the self-cleaning capacity of soil, and has a wide agricultural application prospect.

The invention relates to the technical field of gene engineering, in particular to application of a rice OsSAUR4 gene in regulation and control of rice drought tolerance, and the sequence of the rice OsSAUR4 gene is selected from any one of the following: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a protein sequence as shown in SEQ ID NO.2, and 3) a sequence of which the homology with the nucleotide sequence as shown in SEQ ID NO.1 is more than 90%. The rice OsSAUR4 gene is knocked out, so that the drought tolerance of rice is improved, and the survival rate of rice in a drought stress environment is improved.

The invention relates to the technical field of agriculture, in particular to a preparation method and an application method of a zinc-rich cadmium-reducing yield-increasing foliar fertilizer. The specific technical scheme is as follows: the zinc-rich cadmium-reducing yield-increasing foliar fertilizer is prepared from the following components in percentage by mass: 20 to 30 percent of porous chitosan, 30 to 40 percent of L-malic acid and 40 to 50 percent of nano zinc oxide. The technical problem that in the prior art, the zinc content of rice cannot be increased, meanwhile, the rice yield cannot be increased, and the cadmium content of rice cannot be reduced is solved.

The invention relates to the technical field of agricultural intelligent monitoring and regulation and control, and discloses a rice three-dimensional seedling raising seedling growth monitoring, diagnosis and regulation and control method, which comprises the following steps: S1, when any hanging basket reaches a target position, reducing the movement speed of the hanging basket, and obtaining a serial number image of the hanging basket; s2, when the hanging baskets stop moving, sequentially acquiring images right above and obliquely above all seedling trays in the hanging baskets corresponding to the serial number images of the hanging baskets; s3, respectively obtaining the areas of a green plant area, a yellow plant area, a black soil area and a seedling block area in the images right above and obliquely above the seedling tray, calculating the pixel number of each area, and obtaining the pixel ratio of different areas; and S4, acquiring the coverage rate, health index and spindling index of each seedling tray according to the ratio of pixels in different areas, dividing the seedlings in the seedling trays into five types of normal, spindling, vertical withering, insufficient fertilizer and strong, obtaining a growth vigor diagnosis result of the seedlings in the seedling trays, and generating a seedling growth vigor regulation and control prescription according to the growth vigor diagnosis result.

The invention belongs to the technical field of gene engineering, and provides rice purple acid phosphatase OsPAP16 and application thereof, and the application is that a rice purple acid phosphatase OsPAP16 mutant is applied to rice cultivation. Two OsPAP16 mutants are prepared by adopting a gene editing technology, then the mutants are subjected to overexpression induction, and the result shows that the purple acid phosphatase OsPAP16 is positioned on a cell membrane, the OsPAP16 is subjected to phosphorus deficiency induced expression on leaves and roots, the phosphorus deficiency induced expression at the roots is quicker and higher, the overexpression of the OsPAP16 can improve the activity of the acid phosphatase on the root surface, and the yield of the acid phosphatase on the root surface is increased. Phosphorus deficiency stress of the rice can be relieved by degrading organic phosphorus in the environment, the phosphorus content of the rice is increased, and then growth and development of the rice are promoted.

The invention provides the application of the OsLNHG1 gene in regulating and controlling the nitrogen utilization efficiency and the yield of the rice, in a low-nitrogen paddy field, the plant height of an OsLNHG1 gene overexpression plant is remarkably increased, and the tiller number is a core agronomic character for directly determining the yield of the rice. In a normal nitrogen paddy field, the plant height and the effective tiller number of an OsLNHG1 gene overexpressed plant are remarkably increased, a core gene resource is provided for cultivating a new variety of fertilizer-saving high-yield rice, and the gene has great significance for agricultural sustainable development.