16196 results about "Oryza sativa" patented technology

The invention provides specific transgenic rice plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the rice genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.

Disclosed are a pyrazole amide compound containing diphenyl ether as represented by formula I and/or formula II, application thereof in the prevention and treatment of rice sheath blight and cucumber powdery mildew, and pesticide composition containing the compound. The pyrazole amide compound of the present invention containing diphenyl ether as represented by formula I and/or formula II, and a bactericide containing the pyrazole amide compound as an active ingredient have good prevention and treatment effects on rice sheath blight and cucumber powdery mildew.

Rice plants are disclosed with two separate, but synergistic mechanisms for resistance to herbicides that normally inhibit a plant's acetohydroxyacid synthase (AHAS) enzyme. The herbicide resistance of plants with both resistance mechanisms is substantially greater than one would expect from a simple combination of the two types of resistance. The first of the two resistance mechanisms is a metabolic pathway that is not fully understood, but that does not itself involve a mutant AHAS enzyme. The second resistance mechanism is a mutant AHAS enzyme, an enzyme that shows direct resistance to levels of herbicide that normally inhibit the enzyme, in both in vivo and in vitro assays. Besides controlling red rice, many AHAS-inhibiting herbicides also effectively control other weeds that are common in rice fields. Several of these herbicides have residual activity, so that a treatment controls both existing weeds as well as weeds that sprout later. No herbicide currently available for use on rice has residual activity against a broad spectrum of weeds including red rice. With effective residual activity against red rice and other weeds, rice producers now have a weed control system superior to those currently used.

The present invention provides a rice plant that shows enhanced growth and increased seed production, which also enables reduction of the use of chemical fertilizers.

In the present invention, a nitrogen-fixing endophytic bacterium is isolated from bacteria symbiotically inhabiting natural plants, the isolated endophytic bacterium is artificially proliferated and then artificially inoculated into rice plants, and thus, the nitrogen-fixing endophytic bacteria are allowed to infect to rice plants.

The present invention discloses applications of guaiacol in a rice stripe disease, wherein the applying concentration of the guaiacol liquid is 50-500 [mu]g/mL, and an emulsifying agent is added to the guaiacol liquid as a surfactant. According to the present invention, the guaiacol is used for rice stripe disease prevention and treatment, the rice stripe disease prevention and treatment effect of the guaiacol can achieve 53%, and the guaiacol is combined with the chemical pesticide for virus transmission mediator Laodelphax striatellus prevention and treatment so as to achieve the effect of concurrent disease and pest treatment; and experiment results prove that the guaiacol can be used for effectively preventing and treating the rice stripe disease.

The invention relates to a breeding method for prolongation of rice fertility stage, and the breeding method comprises the following steps: selecting a target fragment in a rice fertility stage determining gene Ehd3 exon region and constructing a plant CRISPR / Cas9 targeting recombinant vector, introducing into rice cells for regeneration to sprout; using an expression frame in the vector to realize shearing of Ehd3 exon region DNA in the rice cells so as to cause self healing of the rice cells; sequencing regeneration strain genome target fragments to obtain an afunction-mutational strain carrying two equipotential Ehd3 genes, and confirming the prolongation of the regeneration plant fertility stage by phenotypic identification. Experiments show that the method can quickly obtain fertility stage prolonged rice materials.

The invention discloses a method for planting se-enriched rice. The method comprises the following steps of land selection and preparation; seeding; seedling culturing; transplanting; field management: intertillage, weeding, topdressing and insect pest prevention; and harvesting. The selenium content of rice planted by using the method is high, and the yield of the rice is also high. Testing confirms that the yield of the se-enriched rice is 620-650kg per acre, and the selenium content of the rice ranges from 0.1 mg/kg to 0.3mg/kg.

The rice plant cultivation and strengthening agent and using method thereof relate to plant cultivation and strengthening agent and using method thereof, which solves the problems of less nutrition content, larger dosage, high production and transport cost, high labor intensity and complexity in fertilization, labor and time consumption in prior rice plant cultivation and strengthening agent. The rice plant cultivation and strengthening agent comprises nutrition elements, flavohumic acid, acid regulating agent, sequestering agent, water conservation agent, disinfectant, plant growth regulator, phlogiston, mould inhibitor, and additive. The using method is that: 1. the rice plant cultivation and strengthening agent is added with water in a proportion of 1 to 1000 times; 2. the solution is applied in the land with a proportion of 500g of the agent to 50m2 of the land; 3. the rice is planted when the agent is fully permeated in the soil. The rice plant cultivation and strengthening agent of the invention has high content of nutrition and the dosage of 10g in 1m2 land, thereby reducing production and transport cost and labor intensity in fertilization. The using of the rice plant cultivation and strengthening agent combines the fertilization, pH regulation, disinfection and first watering together, which is of time and labor saving.

The invention relates to a bacillus amyloliquefaciens growing in a disease-preventing and growth-promoting plant and application thereof, relating to bacillus amyloliquefaciens and the application thereof. The bacillus amyloliquefaciens TF28 growing in the disease-preventing and growth-promoting plant belongs to bacillus, has the storage number of CGMCC No.4038 and the storage data of July 26, 2010. The application of the bacillus amyloliquefaciens TF28 comprises the following steps of: (1) preparing activated bacilli liquid; (2) inoculating the activated bacilli liquid in an NYD culture fluid to obtain a strain fermentation solution; and (3) soaking seeds in a dilution solution 50 times of the strain fermentation solution. The strain TF28 in the invention can be colonized, reproduced and transmitted in plants, can simultaneously generate two antibacterial substances, i.e. antimicrobial proteins and lipopeptide antibiotic and has the multiple efficacy of preventing diseases, stimulating growth, increasing the yield, improving the quality, widening an antibacterial spectrum, and the like. Rice seeds treated by the dilution solution 50 times of the fermentation solution of the strain TF28 reach a room-temperature bakana resistance rate over 84.6 percent.

The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.

The invention relates to construction of rice transgenic materials and aims to provide a gene editing method for knocking out rice MIRNA393b stem-loop sequences with application of a CRISPR(clustered regulatory interspersed short palindromic repeat)-Cas9 system. The gene editing method comprises steps as follows: gRNA target sites are selected for cloning and GG linking, enzyme digestion is performed after amplification, and a product is linked with a pGREB 32 vector; escherichia coli competent cells are transformed; plasmids with a correct sequencing result are used for transforming agrobacteria, transgenic plants are obtained through mediated transformation of rice calli, and transgenic positive lines are obtained; the T0-generation mutant plant seeds are collected for seeding, and the T1-generation plants are subjected to homozygote screening; homozygous lines which are discovered to be negative through MIRNA393b expression are rice mutants completely losing the MIRNA393b stem-loop sequences and MIRNA393b stem-loop sequence expression. According to the gene editing method, MIRNA stem-loop sequences can be effectively knocked out, and loss-of-function mutants of different members in the same MIRNA family can be prepared; the mutant plant propagates to obtain a large number of seeds and is an ideal material for acquiring rice MIRNA393b gene functions successfully.

The invention relates to a method for comprehensively raising various types of aquatic livestock and cultivating paddy in a paddy field, in particular to the raising of Chinese soft-shelled turtles, crawfish and carassius auratus gibelio in the paddy field and the cultivation of paddy. The method comprises the steps of paddy field preparation, aquatic plant transplantation, live food feeding, paddy transplantation, young aquatic livestock placement, bait feeding, daily management, aquatic livestock fishing and paddy harvest. The method for comprehensively raising various types of aquatic livestock and cultivating paddy in the paddy field has the advantages that the various types of aquatic livestock can be raised and paddy can be cultivated in the same field comprehensively, the difference between the period for paddy cultivation and the period for raising aquatic livestock is small, the use rate and the comprehensive efficiency of the field can be effectively improved, and economic benefit can be improved to a large extent; meanwhile, any chemical fertilizer, herbicide and insecticide are not used in the process of production, the produced products can meet the standard of organic food, and finally, edible safety of the products is achieved; the whole process of production is beneficial to conservation of water and soil of the field and the surrounding environment and effectively facilitates the protection of natural ecology.

Provided herein are synergistic herbicidal compositions containing and methods utilizing (a) a compound of formula (I):

or an agriculturally acceptable salt or ester thereof and (b) an ACCase inhibitors, including. e.g., clethodim, clodinafop-propargyl, cyhalofop-R-butyl, diclofop-methyl, fenoxaprop-P-ethyl, fluazifop-P-butyl, haloxyfop-R-methyl, metamifop, pinoxaden, profoxydim, quizalofop-P-ethyl, sethoxydim and tralkoxydim, provide synergistic weed control of undesirable vegetation in rice, cereals, wheat, barley, oats, rye, sorghum, corn/maize, sugarcane, sunflower, oilseed rape, canola, sugar beet, soybean, cotton, pineapple, pastures, grasslands, rangelands, fallowland, turf, tree and vine orchards, aquatics, plantation crops, vegetables, industrial vegetation management (IVM) or rights of way (ROW).

The invention discloses application of CRISPR/nCas9 mediated site-directed base substitution in a plant. The invention provides a plant genome site-directed edition system. The system comprises a BE3 plant expression carrier (expressing a fusion protein composed of nCas9(D10A), deaminase and a uracil DNA glycosylase inhibitory protein), and rice OsPDS and OsSBEIIb are taken as target genes for verifying the system. Results show that an expected site-directed mutant plant is respectively obtained in the three selected target spots, accurate site mutation of a base is realized in rice, and the highest efficiency reaches about 20%, so that a feasible and effective base substitution method is provided for crop breeding, the method has strong application potential in the aspect of agricultural breeding, and a foundation is provided for rapidly improving important agronomic traits of crops.

The invention belongs to the technical field of gene engineering, and concretely relates to a CRISPR/Cpf1 plant genome directional modification function unit, a carrier comprising the function unit, and application thereof. The invention aims to build an effective plant genome CRISPR/Cpf1 directional modification framework carrier according to an action principle of a CRISPR/Cpf1 system and a vegetable cell gene expression characteristic, and realize high-efficient application thereof in plant genome directional modification. The technical scheme provided by the invention is characterized by building the CRISPR/Cpf1 plant genome directional modification framework carrier formed by lachnospiraceae bacterium Cpf1 nuclease protein (LbCpf1) expression unit and a guiding crRNA transcription expression unit. The invention also provides a method for designing specificity-guided crRNA and building a CRISPR/Cpf1 recombinant expression vector aiming at rice genome target loci by adopting the CRISPR/Cpf1 plant genome directional modification framework carrier. The invention provides the high-efficient CRISPR/Cpf1 plant directional modification framework carrier, which can effectively realize simple, fast and high-efficient directional modification based on the CRISPR/Cpf1 system aiming at a plant genome target sequence.

Production of recombinant human serum albumin with rice albuminous cell as biological reactor is carried out by taking rice albuminous cell protein as storage site for recombinant protein, taking rice albuminous specific expression starter and signal peptide, entering inductive recombinant human serum albumin into inner-film system of rice serum albumin cell and storing it in rice albuminous protein. It is cheap, has more yield and higher expression.

The invention relates to a rice seedling raising substrate and a production method thereof, in particular to a rice seedling raising substrate taking agricultural and forestal wastes as materials and mechanically transplanting rice seedlings, and a production method thereof. In the invention, organic wastes such as straws are taken as raw materials, fermented by various beneficial microorganisms, and prepared into an organic substrate with a fertilizer slow release and caking agent and a water retention agent. The rice seedling raising substrate has strong stress resistance and strong compatibility with mechanical rice transplanting technology, and makes the rice seedlings have good quality; and the technology of raising the rice seedlings by the substrate is simple, and has low cost and high success ratio.

The invention relates to a rice field-fishpond planting and breeding compound system, which belongs to the technical field of comprehensive utilization of agricultural ecological aquaculture. The compound system comprises a rice field, a fishpond, a simple ecological ditch and a strengthened ecological ditch, wherein the rice field is arranged at one end of the fishpond; the strengthened ecological ditch and a water collecting tank are respectively arranged on one side of each of the fishpond and the rice field; the simple ecological ditch is formed in the rice field; a small overflow port is formed in the simple ecological ditch; the rice field is communicated with the water collecting tank by a large overflow port; the fishpond is communicated with the simple ecological ditch by a water suction pump. According to the rice field-fishpond planting and breeding compound system disclosed by the invention, the rice field planting and the fishpond breeding are organically combined by the ecological ditches, so that the fishpond breeding water is refluxed to a new circulating water planting-breeding mode after being purified by the rice field and the ecological ditch. Moreover, the problems that an existing rice-orientated planting-breeding mode with the fish breeding has rice-fish contradictions including field drying, fertilizer applying, pest controlling and the like in the production process, and is limited in yield and inconvenient for large-area popularization are solved.

The selenium-rich rice cultivating process includes conventional cultivation and features that the selenium-rich agent is sprayed once during the earing up and flowering period of rice. The selenium-rich agent comprising selenium-containing inorganic compound and phosphate is dissolved in water of 50-90 times and sprayed to leaf of rice during the earing up and flowering period of rice. In case of rainy day after spraying, replenished selenium-rich agent is sprayed timely. The sprayed inorganic selenium will be converted completely into natural organic selenium, and the produced selenium-rich rice is non-toxic, and has the health functions of preventing common cold, preventing and treating hypertension and myocardial pathological changes, etc.