Method for transforming rice into fragrant rice rapidly
A transformation method, rice technology, applied in the field of plant genetic engineering, can solve problems such as unfavorable genes
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[0043] 1. The betaine aldehyde dehydrogenase gene Badh2 was selected for the test. The loss of its function will lead to the increase of 2AP precursor substances, thereby accumulating 2AP and making rice produce aroma. Its gene sequence: GenBank: EU770319.1 (see SEQ ID NO: 1)
[0044] 2. Target design
[0045] Any NGG site in the Badh2 gene sequence can be used as a target to design a gRNA target for gene knockout: the gray background is the PAM sequence.
[0046] first exon selection
[0047] gRNA target site position 1: GCCACGGCGATCCCGCAGCGG
[0048] gRNA target site position 2: GCGGCAGCTCTTCGTCGCCGG
[0049] exon 2 selection
[0050] gRNA target site position 3: ACGTGGACGCGGCGGTGGCGG
[0051] gRNA target site position 4: TGGACGCGGCGGTGGCGGCGG
[0052] gRNA target site position 5: GCGCGGGAGGCGCTGAAGAGG
[0053] gRNA target site position 9: AGTACCTCCGCGCAATCGCGG
[0054] gRNA target site position 10: TCCGCGCAATCGCGGCCAAGG
[0055] Exon 7 selection
[0056] gRNA targ...
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