131 results about "Male individual" patented technology

The invention discloses a breeding method for a new strain of crassostrea gigas with orange left and right shells. The breeding method comprises the following steps of selecting individuals with purple left shells and black palliums from a cultured population to be used as male reproduction brood stocks; selecting individuals with black left shells and black palliums to be used as female reproduction brood stocks; carrying out monogamous insemination to establish an F1 family; selecting female and male individuals with black left and right shells and black palliums from the F1 to be used as reproduction brood stocks and establishing an F2 family; continuously selecting female and male individuals with purple black left and right shells and black palliums from the F2 to be used as reproduction brood stocks and establishing an F3 family; selecting orange individuals as parents of the new strain from the F3 and propagating offspring seeds to culture the new strain of the crassostrea gigas with the orange left and right shells and stable characters. The additional value of products is improved and the market prospect is broad.

The invention discloses a fluorescence labeling composite amplification kit for human Y chromosome 27 STR gene loci, which can simultaneously amplify 27 Y chromosome STR gene loci (including 24 low-mutation-rate Y chromosome STR gene loci and 3 high-mutation-rate Y chromosome STR gene loci) in a single multiplex reaction. By designing the unique gene locus configuration mode and specific primer sequences, the 27 gene loci are divided into 4 groups which are used for different fluorescence labelings. The composite amplification system has the advantages of wide temperature resistance range, high adaptability of the detection material, and high identification capacity for irrelevant and close relative male individuals.

The invention discloses a breeding method for amphiprotic fertile autotetraploid crucian carps and an establishment method for strains of the crucian carps. The breeding method includes the following steps that allotetraploid crucian carps are selected from crucian carp hybridization F1 offspring with red crucian carps as female parents and megalobrama amblycephala as male parents, female individuals capable of generating ova of different sizes and male individuals capable of generating watery semen are selected from the allotetraploid crucian carps for hasten parturition, insemination is carried out manually through a dry method, running water incubation is conducted on fertilized ova, then the fertilized ova are bred, and the amphiprotic fertile autotetraploid crucian carps are selected from selfing offspring. Selfing is carried out on the male individuals and the female individuals in the obtained autotetraploid crucian carps, and the strains of the amphiprotic fertile autotetraploid crucian carps can be established and obtained through multi-offspring breeding. Ploid hybridization is conducted on the autotetraploid crucian carp colonies obtained through the breeding method and diploid crucian carps, so allotriploid fishes can be bred on a large scale. The strains of the crucian carps obtained through the breeding method are excellent in character, stable in heredity and high in fertilization rate and hatchability, and the breeding method has a great significance in genetic research of fishes.

The invention belongs to the technical field of poultry breeding. A method for breeding a new strain of black-bone chicken with higher production performance comprises the following steps of: step 1, taking parents of foreign broiler chicken as female parents instead of hen CD, and the black-bone chicken with jute feather inland as male parents, and generating a generation F1 through cross breeding, selecting and leaving individuals with jute feather, black bone, and black skin in the generation F1; step 2, crossing and propagating the female individuals and the male individuals obtained in the step 1, and separating out jute feather, recessive white feather, black feather, and reed catkins, and removing recessive white feather, from the colored feather colony through crossing, and purifying and fixing the hair color, black bone, black shin, sexual maturity and other appearance characters according to the family and individual selective method, improving the growing speed, reproductive performance and other economic characters; step 3, through the breeding of four or more generations, successfully breeding the new strain of the black-bone chicken with relatively higher production performance in jute feather, recessive white feather, black feather, and reed catkins. The method improves the early growing speed and egg laying performance of the black-bone chicken in the nation, and obviously reduces the feed/gain ratio.

The invention discloses a breeding method for a high-yield new variety line of recessive white-feather gooses and application thereof. The method comprises the following steps of: selecting the high-yield white-feather gooses without broodiness or with weak broodiness, and eliminating dominant white-feather individuals and keeping recessive white-feather individuals A by the test cross or the molecular marker method; self-reproducing the male individuals and female individuals of A to breed the recessive white-feather gooses B; and breeding B again, eliminating dominant white-feather homozygote and heterozygote, self-reproducing the recessive white-feather high-yield individuals with homozygous genotype to expand the group, building a family and breeding the family until the high-yield recessive white-feather goose new variety line C is bred, and performing dualistic or polyphyletic mating by taking the C as a female parent and a colour-feather local goose as a male parent to produce colour-feather gooses or simulated local gooses.

The invention discloses a method for breeding cross-bred 'Hulongban' fish fry. The method comprises: widely crossing blotchy rock cod male individuals serving as female parents and Epinephelus lanceolatus male individuals serving as male parents; and producing cross-bred 'Hulongban' fish fry by artificial induced spawning, insemination, incubation and juvenile fish culture. The method for breeding the 'Hulongban' fish of the invention is simple in operation and suitable for large-scale production; the survival rate of the cultured 'Hulongban' fish fry is 5 to 10 percent; the 'Hulongban' fish fry breeding survival rate is 5 to 10 percent; the culture survival rate is over 95 percent; the survival rates are much higher than those of common grouper; and thus, the method has a promising market prospect.

The invention discloses a primer for sex identification of Nile tilapia. The primer can specifically amplify AMH genes of Nile tilapia, a male individual generates two specifically amplified segments, lengths of the specifically amplified segments are 179bp and 412bp respectively, and a female individual generates a specifically amplified segment of 412bp in length. The primer is used to amplify genome DNA of Nile tilapia, the male individual generates the two specifically amplified segments of 179bp and 412bp in length, the female individual generates the specifically amplified segment of 412bp in length, and the male individual and the female individual can be distinguished obviously, so that sexes of Nile tilapia can be identified simply and accurately. The primer is high in specificity and does not amplify other segments except for a target segment. The invention further discloses a PCR (polymerase chain reaction) identification method using the primer. The PCR identification method is quick to operate and accurate in result, and the defect of unstable results caused by adopting conventional morphological methods to identify Nile tilapia different in sex is avoided.

The invention discloses a human Y chromosome 37 STR loci fluorescence labelled multiplex amplification kit and an application thereof. The kit comprises a specific primer for amplifying 37 Y-STR lociwhich comprise 30 low-mutation Y-STR loci and 7 rapid-mutation Y-STR loci. The kit comprises 30 low-mutation and 7 rapid-mutation rate high-polymorphism Y-STR loci, considers distinguishing of checking of a male family and different male individuals of a same male line, and is the Y-STR detection product with a maximum loci quantity on market. The primer in the kit has the advantages of strong specification, high sensitivity, and accurate parting result, and practical case examination, DNA database construction and paternity identification requirements can be completely satisfied. The kit hasstrong check-material adaptability.

The invention provides a male specific DNA marker of oplegnathus punctatus, wherein the sequence of the DNA fragment on a Y chromosome is shown in SEQ ID NO: 1; the sequence of the DNA fragment on a Xchromosome is shown in SEQ ID NO: 2. The DNA fragment on the Y chromosome is 12 bp more than the fragment on the X chromosome, and the fragment is the DNA fragment peculiar to the Y chromosome. According to the invention, two homologous and different DNA fragments on the X and Y chromosomes are screened from the whole genome sequence of the oplegnathus punctatus, and a method for identifying theheredity sex of the oplegnathus punctatus is further established; and the method can be used for quickly, accurately and effectively distinguishing the heredity sex of the oplegnathus punctatus. According to the method, a specific target band amplified in a male individual cannot be amplified by a female individual, and the product can be resolved by agarose electrophoresis, so that the time for accurately identifying the heredity sex of the oplegnathus punctatus is shortened, and the method is suitable for identifying the heredity sex of the oplegnathus punctatus in a simple environment of aculture farm, and the detection time and the cost are saved. The method has important significance and application value in sex identification, seedling breeding and family selection and breeding of the oplegnathus punctatus.

The present invention relates to a method for eliciting ejaculation in a male individual, comprising delivering one or more stimulation pulses to lumbar spinothalamic (LSt) cells via a light stimulus, wherein said LSt cells express a light-activated cation channel protein.

The invention provides a method and a system for realizing Y-STR typing on the male individual by adopting 26 Y-STR genetic loci. The method comprises the following steps: acquiring DNA of the male individual, and acquiring the genotypes of the 26 Y-STR genetic loci of the DNA, wherein the 26 Y-STR genetic loci are as follows: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS448, DYS456, DYS458, DYS635, GATA H4, DYS438, DYS439, DYS460, DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and DYS533; and acquiring the Y-STR typing result of the male individual according to the genotypes of the 26 genetic loci of the male individual. With the typing result, the operations including family checking, paternity identification, detection for the male element in male and female mixed samples, and the detection for the male element in a plurality of male mixed samples can be realized on the male individual.

The invention provides a method and a system for acquiring the age of a male individual in a Chinese population. The method comprises the following steps: extracting DNA of the male individual; acquiring the methylation rates of 9 CpG sites of the DNA; and according to the methylation rates of the respective CpG loci, performing regression analysis on the 9 CpG loci and the age by R software to construct a regression model so as to acquire the age of the male individual in the Chinese population. According to a scheme provided by the invention, the age of the male individual in the Chinese population can be accurately acquired, and the age of the individual can be inferred from biodetection materials, such as blood or a blood mark sample, which are left on a crime scene in an actual publicsecurity combat, so that quick locking of a criminal suspect scope is facilitated, a clue is provided for case detection and the speed of the case detection is increased.

The invention discloses a skin pattern sex-limited trans-breeding method for a silkworm variety with a common skin pattern. The skin pattern sex-limited trans-breeding method comprises the following steps of: carrying out hybridization to obtain F1 by taking a base skin pattern sex-limited variety as a female parent and taking a practical common skin pattern variety as a male parent; back-crossing the F1 and a male individual of the practical common skin pattern variety to obtain BC1, selecting out the common skin pattern individual which is most easily to discriminate by naked eyes at a larval stage from the BC1, selecting an excellent female individual by discriminating pupas, and continuously back-crossing the excellent female individual to BCn; continuously selecting out the individual with a thick common skin pattern from the BCn, and self-crossing and sub-culturing other progeny individuals to Fm-1, self-crossing and carrying out single batch rearing from Fm, selecting an excellent moth area which is in accordance with skin pattern sex-limited characteristics through a pupa discriminating method to perform subculture, and carrying out sex discrimination on every generation by taking the individuals with skin patterns as the female and taking the individuals without skin patterns as the female. According to the method disclosed by the invention, the practicability of a new variety can be improved by continuous back-crossing; the difficulties that the skin pattern depth of a target individual is difficult to discriminate and most of later generations cannot participate in subculture are solved by adopting a method of selecting out the individual with the thickest skin pattern in the later generations; the process is simplified; the operation is simple; the genetic background is wide; and the aim of realizing rapid practical trans-breeding is fulfilled.

The invention discloses a pseudobagrus ussuriensis genetic male reversal physiological female fish breeding sifting method. The method comprises the steps of incubating fry, selecting testing fry, incubating artemia, soaking artemia with an estradiol solution, regarding artemia as a hormone carrier to be fed to the fry, observing physiological genders of fingerlings and adult fish, and identifyinggenetic genders. Through optimized procedures, a proper feeding starting time, the concentration and time of the estradiol solution for soaking the artemia, the feeding lasting processing time are sifted, the genetically male individuals are genetically reversed into physiologically female individuals, male specific molecular markers are utilized to identify the genetically male physiologically female individuals to be subjected to separate-pond breeding, and breeding is conducted according to the breeding technological path of male fish sexual reversal, artificial gynogenesis, super-male fish and all-male fish. By means of the pseudobagrus ussuriensis genetic male reversal physiological female fish breeding sifting method, a large quantity of high-quality fundamental materials are provided for pseudobagrus ussuriensis all-male-fish breeding within a short period.

The invention provides a method for realizing early sex identification on open-pollinated progenies of the 'Hort 16A' kiwi fruit, belonging to a method for identifying the sex of plants. According to the method, through the screening for the SNP markers, a primer 2-1 capable of realizing stable amplification and realizing differences among male and female plants of the amplification products is determined; the primer is adopted for carrying out PCR amplification on the open-pollinated progenies of the 'Hort 16A' kiwi fruit with the known sex, the sequences among the male and female plants are compared, and the SNP markers capable of identifying the sex are determined; the male No.6 sample (X6) is taken as a contrast, the plants having the t homozygosis at the 59bp position are female individuals, and the plants having the t/c heterozygosis at the 59bp position are male individuals; the plants having the g homozygosis at the 71bp position are the female individuals, the plants having the g/a heterozygosis at the 71bp position are the male individuals. The method is simple, efficient and rapid, and extremely great convenience is provided for the production practice of the 'Hort 16A' kiwi fruit.

The invention discloses a good nacre cultivating method for pinctada martensii. The method comprising the flowing steps: (1) from a certain wild pinctada martensii group, selecting individuals which are larger than 20mm in shell width and larger than 0.35 in ratio of the shell width and the shell height as breeding parents; (2) from the breeding parents, selecting more than 30 female individuals with mature sexual glands and more than 20 male individuals, taking sperms and eggs through cutting, conducting artificial insemination, and breeding spat young shells; (3) consecutively selecting parents according to the step (1) and breeding spat young shells according to the step (2) for more than three generations when bred spat young shells reach 1.5-2.0 years. Consequently, obtained spat young shells can be used for large-scale production breeding and cultivation. The good nacre cultivating method for the pinctada martensii can remarkably improve the forms of shells and enable the shell to be thick, is suitable for core insertion pearl cultivation, and can reduce death and improve pearl cultivation characters.

The invention discloses a quantitative character gene pyramiding breeding method for pinctada fucata. The method comprises the following steps of: collecting at least three pinctada fucata groups from different sources, constructing F1 generation diallel crossing families, evaluating every each family after cultivating for one year, computing an average breeding value, and selecting families of which the breeding values are ranked at first 50 percent for serving as candidate families for constructing a core breeding family, wherein every each combination comprises at least ten families; screening female and male individuals from the candidate families, selecting female and male individuals which are ranked at first 100 places in sizes for measuring, computing individual breeding values, and sequencing; selecting female and male individuals with highest individual breeding values from families with highest female and male breeding values for mating respectively, selecting female and male individuals of which the individual breeding values are ranked at first places from families of which the female and male breeding values are ranked at second places for mating, and so on, and constructing an F2 generation core breeding family; and constructing multiple generations of families including F3, F4, F5 and the like according to the method for breeding generation after generation to over five generations. Through breeding according to the method, the genetic improvement of every each generation can be over 10 percent, the inbreeding coefficient can be controlled at a set level, and inbreeding depression is avoided.

The invention discloses an establishing method for a fish male sterile model. A CRISPR/Cas9 technique is utilized to knock out a meiosis-related gene spo11; heterozygote is fertile; male individuals in a homozygote colony acquired through selfing of heterozygote are sterile, so that the fish male sterile model is established. The sterile male individuals can be specifically acquired according to the method disclosed by the invention; the male sterile individuals can be continuously supplied in the manner of establishing a knockout family; the operation is simple and the time is saved.

The invention discloses a specific molecular marker PCMI-M001 for rapid identification of male seedlings of populus cathayana. A nucleotide sequence of a forward primer PCMI-M001-F of the molecular marker PCMI-M001 is shown as SEQ ID NO:1; a nucleotide sequence of a reverse primer PCMI-M001-R of the molecular marker PCMI-M001 is shown as SEQ ID NO:2; a nucleotide sequence of a specific band obtained by amplification of the marker in a male individual is shown as SEQ ID NO:3. The specific molecular marker (PCMI-M001-F/R) can be used for rapid molecular identification of male and female populuscathayana, and a method is simple, accurate and high in sensitivity and superior to other existing identification methods.

The invention relates to the use of an aromatase inhibitor for the manufacture of a pharmaceutical composition to be used in the treatment of 1) detrusor urethral sphincter dyssynergia in men, wherein said detrusor urethral sphincter dyssynergia is urethral dysfunction which is due to decreased androgen to estrogen ratio in the patient, or 2) decreased androgen to estrogen ratio in men. In addition, the invention also relates to a method for the in vivo investigation of the influence of a certain condition on the urinary function of male individuals using an animal model of male rodents wherein said rodents have been subjected to said condition, anesthetized, provided with a pressure transducer connected to an infusion cannula inserted in the bladder, a flow probe inserted in the distal urethra and connected to a flow meter, infused with a solution into the bladder to induce micturition, and wherein the bladder pressure and urinary flow are registered as function of time. According to the invention, electrode attached to muscles of the lower urinary tract is provided, and that the electrical activity of said muscles are registered simultaneously with the registering of the bladder pressure and the urinary flow.

The invention provides a fluorescence labeling composite amplification kit for detecting 27 human Y chromosome rapid mutation STR loci. 27 STR loci on the Y chromosome with high mutation rate can be amplified multiply in a single reaction. The 27 Y chromosome fast mutant STR loci are divided into four groups by designing unique locus arrangement and specific primers and labeled with different fluorescent dyes. A composite amplification system comprises all the Y chromosome STR loci with high mutation rate and high polymorphism, the male individual recognition capability is high, and the kit can be used as an effective supplement for a conventional Y chromosome STR kit in family segmentation and case investigation. The fluorescence labeling composite amplification system has good primer specificity, strong sample adaptability, simple method operation, short detection time and high efficiency.

The invention discloses a method for evaluating sleep state thermal comfort of male individuals. The metabolic rate is one of major variables of a sleep PMV equation and influences the thermal comfort of human bodies, it is generally acknowledged that the human body metabolic rate in the PMV equation is a constant, but the metabolic rate is variable in different sleep stages of the human bodies. Therefore, the metabolic rates in different sleep stages are required in order to objectively and accurately evaluate the thermal comfort of the human bodies in the sleep process. By monitoring the heart rate value of a testee, the metabolic rate is calculated through the heart rate method, and the sleep PMV equation with the metabolic rate as the variable is obtained. The sleep PMV equation with the metabolic rate as the variable is suitable for sleep state human body thermal comfort evaluation, theoretical bases are provided for sleep environment thermal comfort evaluation, and guidance is provided for creating sleep thermal comfort air conditioner design and generating control strategies.